Papers - KOJIMA Takashi

Reappearance of gap junctions in primary cultures of rat hepatocytes

T MITAKA, T KOJIMA, Y MOCHIZUKI

ELECTRON MICROSCOPY 1994, VOLS 3A AND 3B ( EDITIONS PHYSIQUE )    195 - 196  1994  [Refereed]

Hormonal Regulation of Connexin 43 Expression and Gap Junctional Communication in Human Osteoblastic Cells

Hideki Chiba, Norimasa Sawada, Masahito Oyamada, Takashi Kojima, Michio Mori

Cell Structure and Function   19 ( 3 ) 173 - 177  1994

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We have recently shown that connexin 43 (Cx43), a major gap junction protein in osteoblasts, is expressed with an increase in cell density (CHIBA, H. et al. (1993). Cell Struc. Funct., 18: 419–426). In the present study, we examined what kinds of hormones and cytokines regulate the gap junction protein in osteoblastic cells, using a human osteoblastic cell line (SV-HFO) after reaching a confluent density to avoid influence of cell proliferation. Either retinoic acid (RA) or transforming growth factor-β1 (TGF-β1) induced the Cx43 expression of SV-HFO cells, as revealed by Northern blot analysis and immunocytochemistry. These modulators also increased gap junctional intercellular communication, in terms of the extent of dye transfer. On the other hand, la, 25-dihydroxyvitamin D3 did not influence the Cx43 expression and gap junctional intercellular communication of the cells. These results suggest that RA and TGF-β1 might maintain bone tissue as an organized tissue in vivo by increasing intercellular communication of osteoblastic cells. © 1994, Japan Society for Cell Biology. All rights reserved.

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