研究者詳細 - 一宮　慎吾

論文 - 一宮　慎吾

分割表示 122 件中 101 - 120 件目／全件表示 >>

胸腺上皮ストローマのネットワーク形成と免疫機能調節機構

一宮慎吾, 外岡暁子, 小柴茂, 菊地智樹, 久保輝文, 小島隆, 佐藤昇志

日本病理学会会誌 ( (一社)日本病理学会 ) 95 ( 1 ) 199 - 199 2006年04月

Current progress and perspectives for human tumor immunotherapy

Sato Noriyuki, Hirohashi Yoshihiko, Kamiguchi Kenjiro, Ichimiya Shingo, Sahara Hiroeki, Hirata Koichi, Tsukamoto Taiji, Yamashita Toshihiko, Torigoe Toshihiko

Tumor Research ( Sapporo Medical University ) 41 1 - 13 2006年

The investigation of human tumor immunotherapy has remarkably advanced in the past decade. In our laboratory, human tumor antigens and their HLA-A24-restricted immunogenic peptide epitopes were determined to develop therapeutic and prophylactic human cancer vaccines. Among these peptides, survivin 2B peptide derived from survivin, an inhibitor of apoptosis protein (IAP), is immunogenic in more than 50% of cancer patients with a wide variety of tumors, including colon, pancreas, lung, breast, urinary bladder and oral cancers. It is now under clinical trials, and with careful immunological monitoring we will finally be able to know if these vaccines can work clinically.To develop a potent clinical therapeutic protocol, the immunological tumor escape mechanism should be more thoroughly examined in human tumor materials. To this end, anti-HLA-A, B, and C allele-specific monoclonal antibody EMR8-5, which can be used in routine paraffin-embedded sections, was successfully established. Unexpectedly, our data indicated that a high percentage of human cancers, particularly breast and prostate cancers, lost HLA-class I molecules in their primary cancer tissues. We will discuss possibilities for resolution of this important old but yet new problem.Although recent evidence has been accumulating for an important role of the heat shock proteins (HSPs) as so-called danger signals in initiating innate immunity and consequently activating acquired immunity, the precise immunological basis for this phenomenon remains to be elucidated. Our study indicated that certain HSP-chaperoned immunogenic peptides, particularly HSP90, could efficiently enter the cross-priming pathway in dendritic cells. Interestingly, this cross-priming was TAP-independent and followed endocytic pathways. We also showed that HSP90-chaperoned peptide complexes could work as a potential tumor therapeutic vaccine in the HLA-A24 transgenic mouse model.

DOI CiNii

AIRE-1およびp53ファミリーによるMHCクラスII分子発現調節機構

外岡暁子, 一宮慎吾, 小柴茂, 木村幸子, 横山繁昭, 佐藤昇志

日本免疫学会総会・学術集会記録 ( (NPO)日本免疫学会 ) 35 65 - 65 2005年11月

p53関連分子による胸腺ストローマの細胞間結合制御

小柴茂, 一宮慎吾, 小島隆, 外岡暁子, 村田雅樹, 木村幸子, 横山繁昭, 氷見徹夫, 佐藤昇志

日本免疫学会総会・学術集会記録 ( (NPO)日本免疫学会 ) 35 65 - 65 2005年11月

p63,p73転写因子による胸腺上皮細胞の機能調節機構

外岡暁子, 一宮慎吾, 小柴茂, 木村幸子, 横山繁昭, 佐藤昌明, 佐藤昇志

日本病理学会会誌 ( (一社)日本病理学会 ) 94 ( 1 ) 219 - 219 2005年03月

胸腺ストローマの細胞間結合とその制御

小柴茂, 一宮慎吾, 小島隆, 村田雅樹, 外岡暁子, 木村幸子, 横山繁昭, 氷見徹夫, 佐藤昇志

日本病理学会会誌 ( (一社)日本病理学会 ) 94 ( 1 ) 367 - 367 2005年03月

Napsin A is useful to distinguish primary lung adenocarcinoma from adenocarcinomas of other organs

A Suzuki, N Shijubo, G Yamada, S Ichimiya, M Satoh, S Abe, N Sato

PATHOLOGY RESEARCH AND PRACTICE ( ELSEVIER GMBH, URBAN & FISCHER VERLAG ) 201 ( 8-9 ) 579 - 586 2005年 [査読有り]

The aim of the present study was to compare the usefulness of the peripheral airway cell markers, naspin A and surfactant protein A (SP-A), for distinguishing primary lung adenocarcinoma from adenocarcinomas of other organs in various clinical conditions. Immunohistochemical expression of napsin A and SP-A was analyzed at primary sites of 120 lung carcinomas and 40 adenocarcinomas of other organs, at lung metastatic sites of 32 adenocarcinomas of other organs, and in metastatic lymph nodes of 21 lung adenocarcinomas and 45 adenocarcinomas of other organs. Napsin A and SP-A expressions were compared between primary and recurrent sites in 8 lung adenocarcinomas. Napsin A and SP-A expressions were noted in 84.3% and 53.0% of primary sites of 83 lung adenocarcinomas, respectively, but neither napsin A nor SP-A was expressed at primary sites of other histological types of lung carcinomas or at primary or metastatic sites of adenocarcinomas of other organs. In lung adenocarcinomas, napsin A and SP-A were expressed in metastatic lymph nodes in 81.0% and 19.0%, respectively, and at recurrent sites in 87.5% and 37.5%, respectively. Napsin A is superior to SP-A for distinguishing primary lung adenocarcinoma from adenocarcinomas of other organs at primary, metastatic, and recurrent sites. (c) 2005 Elsevier GmbH. All rights reserved.

DOI PubMed

胸腺上皮細胞におけるHLA分子の発現制御機構

外岡暁子, 一宮慎吾, 小柴茂, 近藤伸彦, 上口権二郎, 田村保明, 鳥越俊彦, 池田英之, 高桑麗子, 横山繁昭, 佐藤昌明, 佐藤昇志

日本免疫学会総会・学術集会記録 ( (NPO)日本免疫学会 ) 34 72 - 72 2004年11月

胸腺スカフォールドの組織構築とその制御

小柴茂, 一宮慎吾, 小島隆, 近藤伸彦, 外岡暁子, 村田雅樹, 高桑麗子, 横山繁昭, 氷見徹夫, 佐藤昇志

日本免疫学会総会・学術集会記録 ( (NPO)日本免疫学会 ) 34 72 - 72 2004年11月

サルコイドーシス(サ症)のTh1反応におけるオステオポンチンの意義の検討

四十坊典晴, 市村志保, 重原克則, 猪股慎一郎, 山田玄, 一宮慎吾, 阿部庄作, 佐藤昇志, 平賀洋明

サルコイドーシス/肉芽腫性疾患 ( 日本サルコイドーシス ) 24 ( Suppl. ) 48 - 48 2004年10月

Role of the p38 MAP-kinase signaling pathway for Cx32 and claudin-1 in the rat liver

Takashi Kojima, Toshinobu Yamamoto, Masaki Murata, Mengdong Lan, Ken-Ichi Takano, Mitsuru Go, Shingo Ichimiya, Hideki Chiba, Norimasa Sawada

Cell Communication and Adhesion 10 ( 4-6 ) 437 - 443 2003年07月 [査読有り]

Liver regeneration and cholestasis are associated with adaptive changes in expression of gap and tight junctions through signal transduction. The roles of stress responsitive MAP-kinase, p38 MAP-kinase, in the signaling pathway for gap junction protein, Cx32, and tight junction protein, claudin-1, were examined in rat liver in vivo and in vitro, including regeneration following partial hepatectomy and cholestasis after common bile duct ligation. Changes in the expression and function of Cx32 and claudin-1 in hepatocytes in vivo were studied using the p38 MAP-kinase inhibitor SB203580. Following partial hepatectomy and common bile duct ligation, down-regulation of Cx32 protein was inhibited by SB203580 treatment. Up-regulation of claudin-1 protein was enhanced by SB203580 treatment after partial hepatectomy but not common bile duct ligation. However, no change of the Ki-67 labeling index (which is a marker for cell proliferation) in the livers treated with SB203580, was observed compared to that without SB203580 treatment. In primary cultures of rat hepatocytes, however, treatment with a p38 MAP-kinase activator, anisomycin, decreased Cx32 and claudin-1 protein levels. p38 MAP-kinase may be an important signaling pathway for regulation of gap and tight junctions in hepatocytes. Changes of gap and tight junctions during liver regeneration and cholestasis are shown to be in part controlled via the p38 MAP-kinase signaling pathway and are independent of cell growth.

PubMed

頭蓋咽頭腫におけるp53ファミリーの発現とその意義

百田洋之, 一宮慎吾, 池田健, 八巻稔明, 宝金清博, 佐藤昇志

Brain Tumor Pathology ( 日本脳腫瘍病理学会 ) 20 ( Suppl. ) 59 - 59 2003年05月

Improved generation of HLA class I/peptide tetramers

Y Sato, H Sahara, T Tsukahara, M Kondo, Y Hirohashi, Y Nabeta, S Kawaguchi, H Ikeda, T Torigoe, S Ichimiya, Y Tamura, T Wada, T Yamashita, M Goto, H Takasu, N Sato

JOURNAL OF IMMUNOLOGICAL METHODS ( ELSEVIER SCIENCE BV ) 271 ( 1-2 ) 177 - 184 2002年12月 [査読有り]

As a tetrameric major histocompatibility complex (MHC) class I-peptide complex (tetramer) is capable of detecting antigen-specific cytotoxic T lymphocyte (CTL) by flow cytometry, significant information about the generation of in vivo immunity can be obtained. It is, however, difficult to make a soluble wild type of MHC class I heavy chain by the prokaryotic expression system. Therefore, we developed a new method for making soluble mutant HLA-A*2402 heavy chain. In this method, signal sequences were deleted, and the codon was changed to silent mutated nucleotide sequences that bacteria could use as preferable codon. When purified mutant HLA-A*2402 molecules were examined for the protein generation by SDS-polyacrylamide gel electrophoresis (PAGE) and western blotting using anti-HLA class I monoclonal antibody (mAb) as compared with wild type, a large amount of mutant heavy chain could be detected. In contrast, the expression of wild-type stable HLA-A*2402 heavy chain molecule was not detected in this system. Consequently, by using mutant HLA-A*2402/peptide tetramers, CTL precursors (CTLp) that specifically recognize antigenic peptide derived from the X;18 chromosomal translocations of synovial sarcoma were detected in patients' PBL. (C) 2002 Elsevier Science B.V. All rights reserved.

DOI PubMed

原発性肺癌患者におけるClara cell 10kilodalton protein(CC10)遺伝子多型についての検討

大地貴, 四十坊典晴, 山田玄, 阿部庄作, 山口昭弘, 一宮慎吾, 佐藤昇志, 河端薫雄, 伊藤喜久

肺癌 ( (NPO)日本肺癌学会 ) 42 ( 5 ) 412 - 412 2002年10月

Comparative analysis of the structure and chromosomal assignment of CD1: an evidence for different evolutionary histories between classic CD1 and CD1D class genes

A Matsuura, M Kinebuchi, S Katabami, HZ Chen, K Kasai, S Ichimiya, K Yamada, MC Yoshida, M Horie, N Sato

JOURNAL OF EXPERIMENTAL ANIMAL SCIENCE ( GUSTAV FISCHER VERLAG ) 41 ( 1-2 ) 87 - 90 2000年03月 [査読有り]

The non-MHC-encoded CD1 family has recently emerged as a novel antigen-presenting system that is distinct from MHC class I and class II molecules. In the present study, we determined the genomic structure of that rat CD1, and compared with those of other previously reported CD1 genes. Rat CD1 was extremely similar to mouse CD1 genes, especially to CD1D1. It is of interest that a tyrosine-based motif for endosomal localization, identified in the human CD1b cytoplasmic tail, was conserved in all CD1 molecules except for CD1a, that was encoded by a single short exon. Comparison of the overall exon-intron organization of CD1 genes revealed that the length of the introns was also characteristic to each of the two classes of CD1 genes; classic (CD1A, CD1B, CD1C and CD1E), and CD1D, which have been categorized by comparison of coding regions. These findings support a hypothesis that the two classes have different evolutionary histories. In contrast to the absence of the classic CD1 genes in rats and mice, the entire region of nonpolymorphic CD1D gene has been conserved through mammalian evolution. Furthermore, we determined chromosomal localization of rat CD1 gene using the fluorescence in situ hybridization method with several probes derived from genomic rat CD1 clones. Similar to human and mouse CD1, rat CD1 mapped outside the MHC loci despite the structural and functional resemblance to MHC. Conserved syntheny of chromosomal segments of RNO2 and MMU3 is implied.

非古典的MHCクラスI分子の役割

Matsuura A, Kinebuchi M, Chen HZ, Ichimiya S, Kasai K

臨床免疫 30 1376 - 1384 1998年 [招待有り]

Interaction of DA41, a DAN-binding protein, with the epidermal growth factor-like protein, S(1-5)

T Ozaki, K Kondo, Y Nakamura, S Ichimiya, A Nakagawara, S Sakiyama

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS ( ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS ) 237 ( 2 ) 245 - 250 1997年08月 [査読有り]

Recently, we have identified a new protein (DA41) which can associate with candidate tumor-suppressor DAN protein. In the present study, we have searched for DA41-interacting protein(s), using a yeast two-hybrid system. An adult rat lung cDNA library was screened by using a truncal;ed form of DA41 (1-308) which lacks a DAN-binding region as bait. One of the positive clones, T16, contained a cDNA sequence of 1934 nucleotides with a single open reading frame of 493 amino acids. A data base search revealed that T16 exhibited a strong sequence similarity to the human epidermal growth factor (EGF)-like protein, S(1-5). The region encoding amino acids 155-232 of DA41 was identified for the interaction with T16. Since DAN and 5(1-5) proteins are known to suppress and stimulate DNA synthesis, respectively, it is possible that functional interaction of DAN with 5(1-5) through DA41 might play an important role(s) in the regulation of cell growth. (C) 1997 Academic Press.

Expression of CD1 molecules but not authentic TL antigens by rat thymus: Possible functional substitution between class Ib molecules in the thymu

Matsuura A, Takayama S, Kinebuchi M, Hashimoto Y, Kasai K, Kozutsumi D, Ichimiya S, Honda R, Natori T, Kikuchi K

Proceedings of International Symposium on Evolution of Network and Molecular Information 1996年 [査読有り]

A new mAb 3B6 distingnishes a novel subpopulation from adult rat bone marrow cells.

Matsuura A, Kinebuchi M, Ichimiya S, Hashimoto Y, Shen M, Katabami S, Kikuchi K

Proceedings of Xth International Workshop on alloantigenic systems in the Rat 10 1994年 [査読有り]

MOLECULAR-CLONING OF A CDNA-ENCODING THE RAT HOMOLOG OF CD1

S ICHIMIYA, A MATSUURA, S TAKAYAMA, K KIKUCHI

TRANSPLANTATION PROCEEDINGS ( ELSEVIER SCIENCE INC ) 25 ( 5 ) 2773 - 2774 1993年10月 [査読有り]

　前のページ - 次のページ　

<一宮 慎吾>

論文 - 一宮 慎吾

<一宮　慎吾>

論文 - 一宮　慎吾