Details of a Researcher - ICHIMIYA Shingo

A Kishi, T Ichinohe, Hirai, I, K Kamiguchi, Y Tamura, M Kinebuchi, T Torigoe, S Ichimiya, N Kondo, K Ishitani, T Yoshikawa, M Kondo, A Matsuura, N Sato

IMMUNOGENETICS ( SPRINGER-VERLAG ) 53 ( 5 ) 401 - 409 2001.07

We previously showed that the cell surface-expressed M-r 70,000 heat shock cognate (hsc70, a constitutively expressed member of the hsp70 family) protein-like molecule (#067 molecule) interacts with rat CD3(+), CD4(-), CD8(-), T-cell receptor (TCR)alpha beta (-), natural killer recetor-Pl(-) T cells. This 70hsc-like molecule was also suggested to present cellular peptide antigens to these T cells. In the present study, we identified the genetic structure of the TCR by establishing T-cell hybridomas between these T cells and mouse BW5147 cells. Our data indicated that these T cells preferentially used TCRs with the V delta6 family. Analysis of the nucleotide sequence of the CDR3 junctional portion showed that there are substantial diversities, with insertion of seven to nine amino acid residues. These data provide indirect evidences for our hypothesis that an hsc70-like molecule could be presented together with cellular peptide antigens to particular T cells with TCR gamma delta chains. Since the expression of this hsc70-like #067 antigen on the cell surface is usually induced along with cell transformation by activated oncogenes, T cells with the TCR V delta6 family are likely to contribute to host resistance to tumor cells.

DOI

A case of inflammatory fibrosarcoma of the retroperitoneum

Atsumori Ueno, Itaru Ozeki, Shigeru Sasaki, Sumio Yamaguchi, Norikazu Iwata, Hiroyuki Kaneto, Fumio Itoh, Takao Endo, Shingo Ichimiya, Masaaki Satoh, Noriyuki Otsuka, Michio Shimizu, Michiaki Matsushita, Satoru Todo, Kohzoh Imai

Japanese Journal of Gastroenterology ( The Japanese Society of Gastroenterology ) 98 ( 4 ) 436 - 441 2001.04

DOI PubMed CiNii

Genetic analysis of p73 localized at chromosome 1p36.3 in primary neuroblastomas

S Ichimiya, Y Nimura, H Kageyama, N Takada, M Sunahara, T Shishikura, Y Nakamura, S Sakiyama, N Seki, M Ohira, Y Kaneko, F McKeon, D Caput, A Nakagawara

MEDICAL AND PEDIATRIC ONCOLOGY ( WILEY-LISS ) 36 ( 1 ) 42 - 44 2001.01

Background. Human p73, a novel homolog of p53, has recently been cloned and mapped at chromosome 1p36.3, the locus for putative tumor suppressor gene(s) of neuroblastoma (NBL) and other cancers, p73, like p53, inhibits growth and induces apoptosis in neuroblastoma and osteosarcoma cell lines. Procedure. To lest the hypothesis that p73 is a NBL suppressor gene, we examined expression, alleto-typing, and mutation of the p73 gene in primary human neuroblastomas. Loss of heterozygosity (LOH) for p73 was performed in 272 primary NBLs using a CT repeat polymorphic marker, which eve found in intron 9 of the p73 gene. Results. p73 LOH was observed in 28 out of 151 (19%) informative cases. The high frequency of p73 LOH was significantly associated with sporadic neuroblastomas (P < 0.001), MYCN amplification (P < 0.001), and advanced stages (P < 0.05). Mutational analyses by PCR-SSCP (single strand conformation polymorphism) revealed two mis-sense mutations in 140 NBLs, one somatic and one germline. Conclusion. Thus, the present results have shown that mutation of p73 is infrequent in NBLs, although the p73 locus is frequently lost in advanced stage rumors. These suggest that p73 may not be a tumor suppressor in the classic Knudson manner. Med. Pediatr. Oncol. 36:42-44, 2001. (C) 2001 Wiley-Liss. Inc.

DOI

Downregulation of hASH1 is associated with the retinoic acid-induced differentiation of human neuroblastoma cell lines

S Ichimiya, Y Nimura, N Seki, T Ozaki, T Nagase, A Nakagawara

MEDICAL AND PEDIATRIC ONCOLOGY ( WILEY-LISS ) 36 ( 1 ) 132 - 134 2001.01

Background. MASH1, a transcription factor with basic helix-loop-helix domain, has a pivotal function to promote differentiation of neural crest cells into autonomic neurons. Procedure. To investigate the functional significance of human MASH1 (hASH1) in the pathogenesis of neuroblastoma, which is originated from autonomic precursor cells, we studied hASH1 gene expression in primary neuroblastomas and human neuroblastoma cell lines. Results. The following results were obtained: (i) hASH1 was expressed in 40 out of 61 (66%) primary neuroblastomas, (ii) hASH1 transcripts were downregulated in several cell lines prior to differentiation induced by all-trans retinoic acid (RA), (iii) a neuroblastoma cell line without expression of endogenous hASH1 did not respond to RA at all, and (iv) the analysis of the hASH1 genomic DNA revealed two possible transcription initiation sites, which may correspond to 3.0 kb and 3.5 kb transcripts. Conclusions. Our observations suggest that, although hASH1 may not preserve the growth capacity of neuroblastomas, downregulation of hASH1 may be necessary to promote neuronal differentiation of neuroblastoma. Med. Pediatr. Oncol. 36:132-134, 2001. (C) 2001 Wiley-Liss. Inc.

DOI

H2 gene complex

Dictionary for Immunology, second edition 2001

H2遺伝子複合体、H2ハプロタイプ、Ia、抗Ia抗体、I-A亜領域、I-E亜領域、I-J亜領域

免疫学辞典第2版 2001

Aberrant Wnt-wingless signaling in gallbladder carcinomas

KIMURA Yasutoshi, FURUHATA Tomohisa, MUKAIYA Mitsuhiro, YANAI Yoshiyuki, KAWAKAMI Masayo, SATOH Masaaki, ICHIMIYA Shingo, HIRAMA Toshinori, HIRATA Koichi

Tando ( Japan Biliary Association ) 14 ( 4 ) 339 - 346 2000.10

To investigate the contribution of β-catenin to the development of gallbladder carcinoma, we searched for genetic alterations of the β-cetenin gene, ctnnb-1. Mutational analysis of exon 3 in ctnnb-1, which encodes the serine/threonine residues for GSK-3β phosphorylation sites was performed for 5 gallbladder cancers with pancreaticobiliary malunion, PMB and 8 noncancerous tissues with PBM. PCR-SSCP analysis showed super-shifted bands in the electrophoretic display, but no mutation was detected through nucleotide sequencing analysis near potential GSK-3βphosphorylation sites. We also performed immunohistochemical analysis of β-catenin protein in all cases, and confirmed accumulation in both the nucleus and cytoplasm in three of 5 cancer tissues, while neighboring noncancerous tissue and the biliary tissue with PBM alone displayed membrane staining. Our results indicated that abnormal Wnt-wingless signaling, resulting in β-catenin accumulations, might be involved in the malignant transformation rather than the development of gallbladder carcinomas.

DOI CiNii

275 Salivary duct carcinomaの3例

東恭悟, 浅沼広子, 太田由紀子, 佐々木雅博, 近藤啓, 遠藤明美, 木村幸子, 一宮慎吾, 池田健, 佐藤昌明

日本臨床細胞学会雑誌 ( 特定非営利活動法人日本臨床細胞学会 ) 39 ( 2 ) 494 - 494 2000.09

CiNii

178 乳腺小葉癌の穿刺吸引細胞診像の検討

太田由紀子, 東恭悟, 浅沼広子, 佐々木雅博, 岸誼博, 木村幸子, 一宮慎吾, 池田健, 佐藤昌明, 成松英明

日本臨床細胞学会雑誌 ( 特定非営利活動法人日本臨床細胞学会 ) 39 ( 2 ) 445 - 445 2000.09

CiNii

肝原発カルチノイド腫ようの1剖検例

小関至, 佐々木茂, 安井寛, 岩田徳和, 三原真美, 金戸宏行, 一宮慎吾, 佐藤昌明, 今井浩三

日本消化器病学会雑誌 97 A676 2000.09

J-GLOBAL

NFBD1/KIAA0170 is a novel nuclear transcriptional transactivator with BRCT domain

T Ozaki, T Nagase, S Ichimiya, N Seki, M Ohiri, N Nomura, N Takada, S Sakiyama, BL Weber, A Nakagawara

DNA AND CELL BIOLOGY ( MARY ANN LIEBERT INC PUBL ) 19 ( 8 ) 475 - 485 2000.08

The BRCT (<(BR)under bar>ECA1 (C) under bar-(t) under bar erminus) superfamily includes a large number of nuclear proteins closely involved in DNA repair, recombination, and cell-cycle control. The human cDNA clone NFBD1 (previously designated KIAA0170) encodes a novel protein (2089 amino acids in length; calculated molecular mass 226,440 D) with possible BRCT domains at its carboxy terminus (amino acid residues 1894-2089), This gene product has been described as one of the BRCT superfamily proteins. However, its biological significance has been unclarified, Expression of green fluorescent protein (GFP)-tagged full-length NFBD1 or a series of deletion mutants indicated that NFBD1 was localized to the nucleus in various mammalian cells, and a 197-amino acid segment near the amino terminus (amino acid residues 142-338) contained a nuclear targeting signal. In vitro DNA-binding experiments showed that the highly basic region of NFBD1 (amino acid residues 1841-1893) possessed DNA-binding activity. The region encoding amino acids 508-995 of NFBD1 fused inframe with GAL4 DNA-binding domain activated transcription in both yeast and mammalian cells, while the possible BRCT domains of NFBD1 failed to induce transcription in mammalian cells. Overexpression of antisense NFBD1 RNA in a p53-deficient human osteogenic sarcoma cell line (SAOS-2) resulted in remarkable suppression of SAOS-2 colony formation. These results suggest that NFBD1 is a nuclear transcriptional transactivator with possible BRCT domains and may contribute to cell growth control.

DOI

p73: Structure and function

S Ichimiya, A Nakagawara, Y Sakuma, S Kimura, T Ikeda, M Satoh, N Takahashi, N Sato, M Mori

PATHOLOGY INTERNATIONAL ( BLACKWELL SCIENCE ASIA ) 50 ( 8 ) 589 - 593 2000.08

Book review, literature introduction, etc.

Alteration of the p53 tumor suppressor gene is a common, if not general, observation in human malignant tumors. p73 Is a novel member of the p53 family at chromosome 1p36.3, at which locus frequent defects are seen in many tumors including neuroblastoma. Besides structural similarities, the fact that p73 functions in the regulation of the cell cycle and apoptosis promotes the expansion of the research field concerning p53-associated tumor progression. In this paper, we review the structure and function of p73 as well as the mutational status in various human tumors. In addition, possibilities for new therapeutic applications with p73 for cancer cell control are discussed.

DOI PubMed

A Case of Bizarre parosteal Osteochondromatous Proliferation of the Femur

KAWAGUCHI S

日本整形外科學會雜誌 74 ( 6 ) S1123 2000.06

CiNii

Identification of the full-length KIAA0591 gene encoding a novel kinesin-related protein which is mapped to the neuroblastoma suppressor gene locus at 1p36.2

M Nagai, S Ichimiya, T Ozaki, N Seki, M Mihara, S Furuta, M Ohira, N Tomioka, N Nomura, S Sakiyama, S Kubo, K Takakura, T Hori, A Nakagawara

INTERNATIONAL JOURNAL OF ONCOLOGY ( PROFESSOR D A SPANDIDOS ) 16 ( 5 ) 907 - 916 2000.05

The distal region of a short arm of chromosome Ip is frequently deleted in many human cancers including neuroblastoma (NBL), in which it has been narrowed down to the smallest region of overlap between D1S244 and D1S214 (similar to 7 cM). During the search for the candidate tumor suppressor genes mapped within the region, we found the KIAA0591 gene which encoded a new human kinesin-related protein with a homology to human axonal transporter of synaptic vesicles (ATSV). The kinesin is an intracellular motor protein and often associated with neuronal differentiation and survival. Here we identified a complete open reading frame of the KIAA0591 gene by screening a cDNA library derived from human substantia nigra. The KIAA0591 protein contains a possible pleckstrin homology (PH) domain at its carboxy-terminus. However, it did not possess a force-generating motor domain which is well conserved among kinesin superfamily members (KIFs). Northern blot analysis demonstrated that KIAA0591 mRNA was preferentially expressed in both adult and fetal brains, kidney, skeletal muscle and pancreas. KIAA0591 was expressed in favorable NBLs at higher levels than in unfavorable NBLs, although RT-PCR SSCP analysis showed no mutation within the coding region of the KIAA0591 gene, when 8 neuroblastoma tissues and 15 neuroblastoma-derived cell lines were examined. Thus, the full-length KIAA0591 gene may be a novel member of human KIF superfamily which lacks motor domain and might function as a tumor suppressor in an epigenetic but not a classic Knudson's manner.

山本直敬, 飯島崇利, 砂原正男, 一宮慎吾, 影山肇, 木村秀樹, 中川原章

日本癌学会総会記事 58th 528 1999.08

J-GLOBAL

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