Misc - 木村　康利

Efficacy of SPIO-MR imaging in the diagnosis of liver metastases from colorectal carcinomas

T Furuhata, K Okita, T Tsuruma, F Hata, Y Kimura, T Katsuramaki, M Mukaiya, N Hirokawa, T Ichimura, N Yama, K Koito, K Sasaki, K Hirata

DIGESTIVE SURGERY ( KARGER )  20 ( 4 ) 321 - 325  2003年

　概要を見る

Aim: To determine whether superparamagnetic iron oxide-enhanced magnetic resonance imaging (SPIO-MRI) could replace intravenous contrast-enhanced spiral CT (iv-CT) and spiral CT during arterial portography (CTAP) combined with spiral CT hepatic angiography (CTHA) in the diagnosis of liver metastases from colorectal carcinomas. Methods: Twenty-six adult patients with liver metastases were studied preoperatively by means of iv-CT, CTAP/CTHA, and SPIO-MRI. Preoperative diagnoses using iv-CT, CTAP/CTHA, and SPIO-MRI were compared with intraoperative and pathological findings in resected specimens. The gold standard for the lesions that were resected was histological examination. Intraoperative findings represented the gold standard for lesions that were not resected. Results: Twenty-six patients were found to have a total number of 43 liver metastases. The sensitivities of iv-CT, CTAP/CTHA, and SPIO-MRI were 74.4, 100, and 90.7%, respectively. SPIO-MRI was significantly superior to iv-CT (p < 0.05). The positive predictive values of iv-CT, CTAP/CTHA, and SPIO-MRI were 97.0, 91.5, and 100%, respectively. CTAP/CTHA yielded four false-positive lesions. In contrast, we detected no false-positive findings using SPIO-MRI. Conclusions: These results suggest that SPIO-MRI might not completely replace CTAP/CTHA, but could replace iv-CT in the diagnosis of liver metastases from colorectal carcinomas. It is thought that SPIO-MRI is a promising imaging modality for diagnosing liver metastases in patients with colorectal carcinoma because of its relatively high sensitivity and extremely high specificity. Copyright (C) 2003 S. Karger AG, Basel.

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Changes in serum levels of apolipoprotein A-1 as an indicator of protein metabolism after hepatectomy

T Katsuramaki, K Hirata, Y Kimura, M Nagayama, M Meguro, H Kimura, T Honma, T Furuhata, U Hideki, F Hata, M Mukaiya

WOUND REPAIR AND REGENERATION ( BLACKWELL PUBLISHING INC )  10 ( 1 ) 77 - 82  2002年01月

　概要を見る

The clinical significance of serum apolipoprotein A-1 levels as an indicator of hepatic protein synthesis after hepatectomy was investigated. A total of 50 patients who had undergone hepatectomy at our department from 1997 to 1999 were selected for this study, The serum levels of apolipoprotein A-1, indocyanine green dye retention at 15 minutes, lectin-cholesterol acyltransferase, prealbumin, and high-density lipoprotein cholesterol were measured in these patients preoperatively and on postoperative days 7 and 14, The type of hepatic resection conducted was partial resection in 13 cases, subsegmentectomy in 13 cases, segmentectomy in five cases, and bisegmentectomy in 19 cases, All the patients tolerated the operation, and none of the cases had any severe complications, such as liver failure, In most cases, the serum apolipoprotein A-1 levels decreased on postoperative day 7 and recovered by day 14, There were no significant differences in the changes in apolipoprotein A-1 levels between patients with the individual types of operative procedures. The serum apolipoprotein A-1 levels showed significant correlations with the serum high-density lipoprotein cholesterol, lectin-cholesterol acyltransferase and prealbumin levels on postoperative days 7 and 14, however, there was no significant correlation with the indocyanine green retention test, When the cases were divided into three groups according to the serum level of apolipoprotein A-1 on postoperative day 7 (group A: over 81 mg/dl, group B: 61-80 mg/dl, group C: under 60 mg/dl), the serum indocyanine green retention, prealbumin, lectin-cholesterol acyltransferase and high-density lipoprotein cholesterol levels in group C were significantly lower than those in group A on postoperative day 7. On the basis of these results, it is suggested that the pattern of changes in the serum apolipoprotein A-1 levels may be a good indicator of the hepatic protein synthetic ability during the perioperative period after hepatectomy.

DOI

[A case of recurrent hepatocellular carcinoma successfully treated with a combination therapy of interferon-alpha and intravenous continuous infusion of 5-fluorouracil]

Gan To Kagaku Ryoho   29   1801 - 4  2002年

p53 とは何か

Digestive Surgery   6   2 - 3  2002年

beta-Catenin alteration in cancer of the ampulla of Vater.

J Exp Clin Cancer Res   21   23 - 7  2002年

大腸癌術後に発症した遷延性ダグラス窩膿瘍の一例

外科感染症研究会雑誌   14   157 - 162  2002年

Histopathological characteristics of biliary tract carcinomas arising from the pancreaticobiliary maljunction

Pancreaticobiliary Maljunction    2002年

Obstructive jaundice due to internal herniation: a case report and review of the literature.

Hepatogastroenterology   49   1030 - 2  2002年

Whatﾕs new in SURGERY FRONTIER -c-ets-1-

SURGERY FRONTIER   8   73 - 76  2001年

Vascular endothelial growth factor C promotes human gastric carcinoma lymph node metastasis in mice.

J Exp Clin Cancer Res   20   419 - 28  2001年

膵管胆道合流異常を伴った胆嚢癌におけるWnt-wingless signalingの異常

木村 康利, 古畑 智久, 向谷 充宏, 柳内 良之, 川上 雅代, 佐藤 昌明, 一宮 慎吾, 平間 敏憲, 平田 公一

胆道 ( 日本胆道学会 )  14 ( 4 ) 339 - 346  2000年

　概要を見る

胆嚢癌の発癌,進展に関与する癌関連遺伝子の検索を目的に,β-catenin蛋白をコードするctnnb-1遺伝子の変異検索を行った.ctnnb-1遺伝子のexon 3はGSK-3βによるリン酸化部位であり,これをPCR-SSCP法にて解析した.さらに,免疫組織化学的検討を行いβ-catenin蛋白の細胞内局在の異常を解析した. 対象は膵管胆道合流異常を合併した胆嚢癌5例と胆嚢癌の合併のない合流異常症8例の胆嚢である.その結果,胆嚢癌5例中3例に,遺伝子変異を同定し得なかったものの,β-catenin蛋白の核,または細胞質への移行が確認された.合流異常症例の胆道系組織(非癌部)では,遺伝子変異,細胞内局在をともに認めなかったことから,Wnt-winglessの情報伝達系におけるβ-catenin蛋白の異常蓄積は胆嚢癌の発生,進展において北較的後期に関与する可能性が示唆された.

DOI CiNii

肝切除術の基本手技

外科治療   83   698 - 706  2000年

切除不能進行膵癌に対する大内臓神経切離術の経験

北海道外科   45   49 - 53  2000年

転移性膵体部腫瘍に対し膵体部横断切除後、膵管膵管吻合で再建しえた一例

手術   54   1919 - 1923  2000年

病態に応じた輸液 (8)代謝性アルカローシスの場合

臨床外科   55   591 - 593  2000年

DOI

GML sensitizes cancer cells to Taxol by induction of apoptosis

Y Kimura, T Furuhata, T Shiratsuchi, H Nishimori, K Hirata, Y Nakamura, T Tokino

ONCOGENE ( STOCKTON PRESS )  15 ( 11 ) 1369 - 1374  1997年09月

　概要を見る

Recently we identified a novel gene, gml, whose expression is regulated in a p53-dependent manner and found that gml expression was correlated with the sensitivity of esophageal cancer cells to anticancer drugs. To further investigate the biological mechanism of gml in determining the chemosensitivity of cancer cells to clinically useful agents, we introduced gml cDNA into TE10, an esophageal cancer cell line that lacks endogenous gml expression. In two resulting stable cell lines which expressed gml cDNA in the absence of wild-type p53, cell death occurred within 6 h after treatment with Taxol. TE10 parent cells or TE10 cells transfected with vector alone displayed relative resistance for 36 h. Induction of gml did not by itself affect viability. Morphological analysis confirmed that the increased chemosensitivity to Taxol conferred by gml was due to apoptosis. These data suggest that reduced expression of gml is likely to be associated with poor response rates to chemotherapy, and that an assay for gml expression might serve a clinical purpose as a predictor of chemotherapeutic sensitivity.

DOI PubMed CiNii

Genomic structure and chromosomal localization of GML (GPI-anchored molecule-like protein), a gene induced by p53

Y Kimura, T Furuhata, T Urano, K Hirata, Y Nakamura, T Tokino

GENOMICS ( ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS )  41 ( 3 ) 477 - 480  1997年05月

　概要を見る

Among its known functions, tumor suppressor gene p53 serves as a transcriptional regulator and mediates various signals through activation of downstream genes, We recently identified a novel gene, GML (glycosylphosphatidylinositol (GPI)-anchored molecule-like protein), whose expression is specifically induced by wildtype p53, To characterize the GML gene further, we determined 35.8 kb of DNA sequence that included a consensus binding sequence for p53 and the entire GML gene. The GML gene consists of four exons, and the p53-binding sequence is present in the 5'-flanking region. in genomic organization this gene resembles genes encoding murine Ly-6 glycoproteins, a human homologue of the Ly-6 family called RIG-E, and CD59; products of these genes, known as GPI-anchored proteins, are variously involved in signal transduction, cell-cell adhesion, and cell-matrix attachment. FISH analysis revealed that the GML gene is located on human chromosome 8q24.3. Genes encoding at least two other GPI-anchored molecules, E48 and RIG-E, are also located in this region. (C) 1997 Academic Press.

DOI PubMed CiNii