KOBUNE Masayoshi

写真a

Affiliation

School of Medicine, Department of Hematology

Job title

Associate Professor
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Degree 【 display / non-display 】

  • Internal Medicine

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Professional Memberships 【 display / non-display 】

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    日本消化器病学会

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    国際鉄代謝学会

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    日本肝臓学会

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    日本再生医療学会

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    International society of iron metabolism

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Affiliation 【 display / non-display 】

  • Sapporo Medical University   School of Medicine, 4th Dept.of Internal Medicine   Instructor  

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Research Interests 【 display / non-display 】

  • 分子血液学 再生医療 遺伝子治療

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Misc 【 display / non-display 】

  • Ex vivo expansion of G-CSF-mobilized peripheral blood CD133(+) progenitor cells on coculture with human stromal cells

    Y Kawano, M Kobune, H Chiba, K Nakamura, R Takimoto, K Takada, Y Ito, J Kato, H Hamada, Y Niitsu

    EXPERIMENTAL HEMATOLOGY ( ELSEVIER SCIENCE INC )  34 ( 2 ) 150 - 158  2006.02

    　View Summary

    Objective. The pentaspan molecule CD133 has been shown to be a marker of more primitive hematopoietic progenitors in mobilized peripheral blood (PB). Our objective was to assess the efficacy of PB CD133(+) cells in our coculture system using human telomerized stromal (HTS) cells. Methods. Five thousand PB CD133(+) cells or conventional cord blood (CB) CD34(+) cells were expanded with or without HTS cells in the presence or absence of stem cell factor, thrombopoietin, and Flk-2/Flt-3 ligand. Results. The coculture was significantly superior in expanding PB clonogenic cells as compared with the stroma-free culture (CFU-C, 2 +/- 0 vs 111 +/- 15-fold of initial cell number p < 0.01), and the fold increase of PB clonogenic cells was comparable to that for CB cells after two weeks of coculture (BFU-E, 54 +/- 3 vs 56 +/- 4-fold; CFU-GM, 156 +/- 26 vs 83 +/- 9-fold; CFU-Mix, 30 +/- 11 vs 80 +/- 36-fold). However, proliferation of CFU-Mk from PB on coculture with HTS cells was modest as compared with stroma-free culture. Concomitantly, multiple hematopoietic cells transmigrated below the stromal layer and formed cobblestone areas (CAs). The production of hematopoietic progenitor cells from CAs after coculture with PB was significantly lower than that seen in cells cocultured with CB for four weeks (CFU-Mix, 0 +/- 0 vs 9 +/- 5-fold on day 28, p < 0.01), although a similar number of CAs derived from PB and CB were observed. Conclusion. PB CD133(+) cells proliferated efficiently above the stromal layer, while the characteristics of PB CD133(+) cells underneath the human stromal layer were likely to be maintained, even after long-term hematopoietic-stromal interaction. (C) 2006 International Society for Experimental Hematology. Published by Elsevier Inc.

    DOI

  • 今日の治療指針

    医学書院    2006

  • Konnichi no tiryousisin

    Igaku syoin    2006

  • 3 Disorder of Red Blood Cells, A. Anemia, a Iron-deficiency anemia

    Miwa hematology    2006

  • 臨床各論．3 赤血球の疾患 A．貧血 a 鉄欠乏性貧血

    三輪血液病学    2006

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Research Projects 【 display / non-display 】

  • 骨髄および臍帯血由来間葉系幹細胞を用いた再生医療

  • Wnt/Hedgehogシグナルによる造血器腫瘍の治療抵抗性誘導機構の解明

  • 造血幹細胞の遺伝子改変を安全に行うための治療用ベクターの開発

  • 骨髄由来幹細胞の分化制御と大量培養系の確立

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