Papers - TANNO Masaya

Involvement of necroptosis in contrast-induced nephropathy in a rat CKD model.

Satoru Shibata, Norihito Moniwa, Atsushi Kuno, Ayumu Kimura, Wataru Ohwada, Hirohito Sugawara, Yufu Gocho, Marenao Tanaka, Toshiyuki Yano, Masato Furuhashi, Masaya Tanno, Takayuki Miki, Tetsuji Miura

Clinical and experimental nephrology   25 ( 7 ) 708 - 717  2021.07  [Refereed]  [Domestic journal]

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BACKGROUND: The risk of contrast-induced nephropathy (CIN) is high in patients with chronic kidney disease (CKD). However, the mechanism of CIN in CKD is not fully understood. Here, we prepared a clinically relevant model of CIN and examined the role of necroptosis, which potentially cross-talks with autophagy, in CIN. METHODS: In Sprague-Dawley rats, CKD was induced by subtotal nephrectomy (SNx, 5/6 nephrectomy) 4 weeks before induction of CIN. CIN was induced by administration of a contrast medium (CM), iohexol, following administration of indomethacin and N-omega-Nitro-L-arginine methyl ester. Renal function and tissue injuries were assessed 48 h after CM injection. RESULTS: Serum creatinine (s-Cre) and BUN were increased from 0.28 ± 0.01 to 0.52 ± 0.02 mg/dl and from 15.1 ± 0.7 to 29.2 ± 1.2 mg/dl, respectively, after SNx alone. CM further increased s-Cre and BUN to 0.69 ± 0.03 and 37.2 ± 2.1, respectively. In the renal tissue after CM injection, protein levels of receptor-interacting serine/threonine-protein kinase (RIP) 1, RIP3, cleaved caspase 3, and caspase 8 were increased by 64 ~ 212%, while there was reduction in LC3-II and accumulation of p62. Necrostatin-1, an RIP1 inhibitor, administered before and 24 h after CM injection significantly suppressed elevation of s-Cre, BUN and urinary albumin levels, kidney injury molecule-1 expression and infiltration of CD68-positive macrophages in renal tissues after CM injection. CONCLUSION: The results suggest that necroptosis of proximal tubular cells contributes to CIN in CKD and that suppression of protective autophagy by pro-necroptotic signaling may also be involved.

DOI PubMed

Characteristics of patients with emergency attendance for severe hypoglycemia and hyperglycemia in a general hospital in Japan

Keisuke Endo, Takahito Itoh, Masaya Tanno, Kouhei Ohno, Hiroyuki Hotta, Nobuo Kato, Tomoaki Matsumoto, Hitoshi Ooiwa, Hirofumi Kubo, Takayuki Miki

Medicine ( NLM (Medline) )  100 ( 25 ) e26505  2021.06

DOI PubMed

ミトコンドリア関連ER膜および細胞質におけるAMPデアミナーゼの上昇は糖尿病心の圧負荷による心機能障害に大きく寄与する(Upregulated AMP Deaminase in Mitochondria-associated ER Membranes and Cytosol Distinctively Contributes to Pressure Overload-induced Cardiac Dysfunction in Diabetic Hearts)

Tanno Masaya, Osanami Arata, Kouzu Hidemichi, Ogawa Toshifumi, Kuno Atsushi, Miura Tetsuji

日本循環器学会学術集会抄録集 ( (一社)日本循環器学会 )  85回   SS14 - 4  2021.03

院外心停止蘇生後患者における血液凝固/線溶異常の予後的意義(Prognostic Value of Abnormalities in the Coagulation/Fibrinolysis Status in Patients after Resuscitation from Out-of-Hospital Cardiac Arrest)

中田 潤, 國分 宣明, 永野 伸卓, 西川 諒, 高橋 遼, 岸上 直広, 大友 俊作, 小山 雅之, 丹野 雅也, 三浦 哲嗣

日本循環器学会学術集会抄録集 ( (一社)日本循環器学会 )  85回   OJ26 - 6  2021.03

糖尿病腎の心腎症候群type 1への感受性増加におけるTLRs/NOXsシグナル伝達とオートファジー障害の影響(Detrimental Roles of TLRs/NOXs Signaling and Impaired Autophagy in Increased Susceptibility of the Diabetic Kidney to Type 1 Cardiorenal Syndrome)

Kuno Atsushi, Sato Tatsuya, Kouzu Hidemichi, Yano Toshiyuki, Tanno Masaya, Miura Tetsuji

日本循環器学会学術集会抄録集 ( (一社)日本循環器学会 )  85回   PL09 - 1  2021.03

Mitochondrial associated ER membraneにおけるAMPD3の役割とミトコンドリア膜透過性遷移の閾値制御(Role of AMPD3 in Mitochondria-associated ER Membranes in Regulation of Threshold for Mitochondrial Permeability Transition)

Osanami Arata, Tanno Masaya, Kuno Atsushi, Kouzu Hidemichi, Yano Toshiyuki, Ogawa Toshifumi, Sato Tatsuya, Miura Tetsuji

日本循環器学会学術集会抄録集 ( (一社)日本循環器学会 )  85回   OE080 - 6  2021.03

AMPデアミナーゼの細胞内局在化とXORによるROS生成におけるCytosolic AMPDの役割(Intracellular Localization of AMP Deaminase and a Role of Cytosolic AMPD in XOR-mediated ROS Production)

Osanami Arata, Tanno Masaya, Kuno Atsushi, Kouzu Hidemichi, Yano Toshiyuki, Ogawa Toshifumi, Sato Tatsuya, Miura Tetsuji

日本循環器学会学術集会抄録集 ( (一社)日本循環器学会 )  85回   OE081 - 6  2021.03

Prognostic Value of Abnormalities in the Coagulation/Fibrinolysis Status in Patients after Resuscitation from Out-of-Hospital Cardiac Arrest(和訳中)

中田 潤, 國分 宣明, 永野 伸卓, 西川 諒, 高橋 遼, 岸上 直広, 大友 俊作, 小山 雅之, 丹野 雅也, 三浦 哲嗣

日本循環器学会学術集会抄録集 ( (一社)日本循環器学会 )  85回   OJ26 - 6  2021.03

Xanthine oxidoreductase-mediated injury is amplified by upregulated AMP deaminase in type 2 diabetic rat hearts under the condition of pressure overload.

Yusuke Igaki, Masaya Tanno, Tatsuya Sato, Hidemichi Kouzu, Toshifumi Ogawa, Arata Osanami, Toshiyuki Yano, Atsushi Kuno, Takayuki Miki, Takashi Nakamura, Tetsuji Miura

Journal of molecular and cellular cardiology   154   21 - 31  2021.02  [Refereed]  [International journal]

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BACKGROUND: We previously reported that upregulated AMP deaminase (AMPD) contributes to diastolic ventricular dysfunction via depletion of the adenine nucleotide pool in a rat model of type 2 diabetes (T2DM), Otsuka Long-Evans-Tokushima Fatty rats (OLETF). Meanwhile, AMPD promotes the formation of substrates of xanthine oxidoreductase (XOR), which produces ROS as a byproduct. Here, we tested the hypothesis that a functional link between upregulated AMPD and XOR is involved in ventricular dysfunction in T2DM rats. METHODS AND RESULTS: Pressure-volume loop analysis revealed that pressure overloading by phenylephrine infusion induced severer left ventricular diastolic dysfunction (tau: 14.7 ± 0.8 vs 12.5 ± 0.7 msec, left ventricular end-diastolic pressure: 18.3 ± 1.5 vs 12.2 ± 1.3 mmHg, p < 0.05) and ventricular-arterial uncoupling in OLETF than in LETO, non-diabetic rats, though the baseline parameters were comparable in the two groups. While the pressure overload did not affect AMPD activity, it increased XOR activity both in OLETF and LETO, with OLETF showing significantly higher XOR activity than that in LETO (347.2 ± 17.9 vs 243.2 ± 6.1 μg/min/mg). Under the condition of pressure overload, myocardial ATP level was lower, and levels of xanthine and uric acid were higher in OLETF than in LETO. Addition of exogenous inosine, a product of AMP deamination, to the heart homogenates augmented XOR activity. OLETF showed 68% higher tissue ROS levels and 47% reduction in mitochondrial state 3 respiration compared with those in LETO. Overexpression of AMPD3 in H9c2 cells elevated levels of hypoxanthine and ROS and reduced the level of ATP. Inhibition of XOR suppressed the production of tissue ROS and mitochondrial dysfunction and improved ventricular function under the condition of pressure overload in OLETF. CONCLUSIONS: The results suggest that increases in the activity of XOR and the formation of XOR substrates by upregulated AMPD contribute to ROS-mediated diastolic ventricular dysfunction at the time of increased cardiac workload in diabetic hearts.

DOI PubMed

Activation of the angiotensin II receptor promotes autophagy in renal proximal tubular cells and affords protection from ischemia/reperfusion injury

Hirohito Sugawara, Norihito Moniwa, Atsushi Kuno, Wataru Ohwada, Arata Osanami, Satoru Shibata, Yukishige Kimura, Koki Abe, Yufu Gocho, Masaya Tanno, Tetsuji Miura

Journal of Pharmacological Sciences ( Elsevier BV )  145 ( 2 ) 187 - 197  2021.02  [Refereed]

DOI

経口ブドウ糖負荷試験で診断し得た機能性低血糖症の1例

寺沢 誠, 長南 新太, 佐藤 達也, 原田 なお, 川原田 航, 箱崎 頌平, 後町 結, 茂庭 仁人, 矢野 俊之, 古橋 眞人, 丹野 雅也, 三浦 哲嗣

日本内分泌学会雑誌 ( (一社)日本内分泌学会 )  96 ( 3 ) 596 - 596  2021.01

Distinct intra-mitochondrial localizations of pro-survival kinases and regulation of their functions by DUSP5 and PHLPP-1

Wataru Ohwada, Masaya Tanno, Toshiyuki Yano, Sang-Bing Ong, Koki Abe, Tatsuya Sato, Atsushi Kuno, Takayuki Miki, Hirohito Sugawara, Yusuke Igaki, Tetsuji Miura

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease ( Elsevier BV )  1866 ( 10 ) 165851 - 165851  2020.10  [Refereed]

DOI

非専門医の外来診療における慢性合併症評価率の年次変化

小川 俊史, 佐藤 達也, 三木 隆幸, 東浦 幸村, 伊野 祥哉, 矢野 俊之, 古橋 眞人, 神津 英至, 丹野 雅也, 斎藤 重幸, 三浦 哲嗣

糖尿病 ( (一社)日本糖尿病学会 )  63 ( Suppl.1 ) S - 204  2020.08

ドキソルビシン誘発性心筋症の発症には鉄結合タンパク質ではなく遊離鉄のミトコンドリアへの蓄積が関与している可能性がある(Accumulation of Free Iron, but not Protein-bound Iron, in Mitochondria may Contribute to Development of Doxorubicin-induced Cardiomyopathy)

Sato Tatsuya, Kikuchi Tatsuya, Yano Toshiyuki, Miki Takayuki, Tanno Masaya, Kuno Atsushi, Kouzu Hidemichi, Miura Tetsuji

日本循環器学会学術集会抄録集 ( (一社)日本循環器学会 )  84回   PE48 - 7  2020.07

非虚血性拡張型心筋症において核内および介在板に局在するnecroptosisの実行因子リン酸化MLKLは各々独立して働いている(Phosphorylated MLKL, an Executor of Necroptosis, Localized in the Nucleus and Intercalated Disc Plays Distinct Roles in Nonischemic Dilated Cardiomyopathy)

Fujita Yugo, Yano Toshiyuki, Nagano Nobutaka, Nishikawa Ryo, Kamiyama Naoyuki, Fujito Takefumi, Mochizuki Atsushi, Koyama Masayuki, Kouzu Hidemichi, Muranaka Atsuko, Nagahara Daigo, Tanno Masaya, Miki Takayuki, Miura Tetsuji

日本循環器学会学術集会抄録集 ( (一社)日本循環器学会 )  84回   OE61 - 5  2020.07

糖尿病外来診療における慢性合併症評価の実態調査

小川 俊史, 佐藤 達也, 東浦 幸村, 伊野 祥哉, 矢野 俊之, 古橋 眞人, 丹野 雅也, 三木 隆幸, 斎藤 重幸, 三浦 哲嗣

糖尿病 ( (一社)日本糖尿病学会 )  63 ( 5 ) 356 - 356  2020.05

mTORC1 inhibition attenuates necroptosis through RIP1 inhibition-mediated TFEB activation

Abe K, Yano T, Tanno M, Miki T, Kuno A, Sato T, Kouzu H, Nakata K, Ohwada W, Kimura Y, Sugawara H, Shibata S, Igaki Y, Ino S, Miura T

BBA Molecular Basis of Disease   1865 ( 12 ) 165552  2019.12  [Refereed]

DOI PubMed

心不全と心腎連関における髄質集合管バソプレシンV2受容体の役割 BOREAS-ADHFレジストリ解析結果より

丹野 雅也, 永野 伸卓, 能登 貴弘, 長南 新太, 神津 英至, 三浦 哲嗣

日本心臓病学会学術集会抄録 ( (一社)日本心臓病学会 )  67回   SS - 3  2019.09

Canagliflozin, an SGLT2 inhibitor, normalizes renal susceptibility to type 1 cardiorenal syndrome through reduction of renal oxidative stress in diabetic rats.

Kimura Y, Kuno A, Tanno M, Sato T, Ohno K, Shibata S, Nakata K, Sugawara H, Abe K, Igaki Y, Yano T, Miki T, Miura T

Journal of Diabetes Investigation   10 ( 4 ) 933 - 946  2019.07  [Refereed]

DOI PubMed

mTORC1活性阻害はRIP1の抑制的リン酸化により心筋細胞のネクロプトーシスを抑制する

矢野 俊之, 安倍 功記, 佐藤 達也, 神津 英至, 久野 篤史, 丹野 雅也, 三木 隆幸, 三浦 哲嗣

日本循環制御医学会総会プログラム・抄録集 ( 日本循環制御医学会 )  40回   63 - 63  2019.06