Details of a Researcher - TAKAHASHI Satoshi

Misc - TAKAHASHI Satoshi

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新型コロナウイルス感染症専用病棟の職員の感染リスクにかかる後向きコホート研究

藤谷好弘, 中江舞美, 中村広士, 黒沼幸治, 高橋聡

日本環境感染学会総会プログラム・抄録集 ( (一社)日本環境感染学会 ) 36回 303 - 303 2021.09

COVID-19ワクチン接種後感染者1事例の抗体獲得推移

中江舞美, 藤谷好弘, 藤居賢, 黒沼幸治, 高橋聡

日本環境感染学会総会プログラム・抄録集 ( (一社)日本環境感染学会 ) 36回 311 - 311 2021.09

鼻腔ぬぐい液および抗原定量検査の臨床的有効性に関する検討

藤谷好弘, 黒沼幸治, 高橋聡

感染症学雑誌 ( (一社)日本感染症学会 ) 95 ( 臨増 ) 251 - 251 2021.04

新型コロナウイルス感染症におけるファビピラビル投与の評価

伊部裕太, 藤居賢, 石郷友之, 國本雄介, 藤谷好弘, 黒沼幸治, 高橋聡

日本化学療法学会雑誌 ( (公社)日本化学療法学会 ) 69 ( Suppl.A ) 254 - 254 2021.04

流産手術後に長期間の反復菌血症を呈しミュンヒハウゼン症候群が疑われた1例

真里谷奨, 長井陽子, 藤谷好弘, 黒沼幸治, 高橋聡

感染症学雑誌 ( (一社)日本感染症学会 ) 95 ( 臨増 ) 288 - 288 2021.04

嗅覚障害が持続する新型コロナウイルス感染症患者は退院後も経過に注意すべきである

藤谷好弘, 黒沼幸治, 高橋聡

日本化学療法学会雑誌 ( (公社)日本化学療法学会 ) 68 ( Suppl.A ) 302 - 302 2020.09

Fosfomycinによる術前除菌が無効であった尿道損傷患者の尿から検出されたESBL産生大腸菌の細菌学的検討

桧山佳樹, 佐藤豊孝, 高橋聡, 舛森直哉, 横田伸一

日本化学療法学会雑誌 ( (公社)日本化学療法学会 ) 68 ( 3 ) 451 - 451 2020.05

J-GLOBAL

淋菌に対するpiperacillinとtazobactam/piperacillinの薬剤感受性の検討

桧山佳樹, 高橋聡, 佐藤豊孝, 山本聡, 品川雅明, 横田伸一, 舛森直哉

日本性感染症学会誌 ( (一社)日本性感染症学会 ) 29 ( 2 ) 236 - 236 2018.10

前立腺癌に対するIMRTを用いた全骨盤照射の安全性

堀正和, 中田健生, 染谷正則, 北村寛, 高橋聡, 舛森直哉, 坂田耕一

泌尿器外科 ( 医学図書出版(株) ) 28 ( 8 ) 1391 - 1393 2015.08

骨盤内リンパ節転移を有するN1前立腺癌症例では、局所照射に加え、転移リンパ節に対して照射を行う場合を経験する。当科では、D1症例や超高リスク症例、複数の高リスク因子を有する症例に対して、強度変調放射線治療IMRTを用いた全骨盤照射を導入している。これまで12例の症例に対してIMRTを用いて全骨盤照射を行っており、許容範囲内の急性期反応で全例休止なく治療を完遂している。IMRTを用いた全骨盤照射は忍容性の高い治療と考えられた。(著者抄録)

排尿時に痛みがあり、熱がある (特集泌尿器科外来で覚えておきたい症状アセスメント : 患者さんの訴えから診断までの流れがわかる)

橋本次朗, 高橋聡, 舛森直哉

泌尿器ケア ( メディカ出版 ) 20 ( 4 ) 426 - 430 2015.04

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膀胱におけるサーファクタント蛋白質Aの発現と尿路病原性大腸菌に対する感染防御機構

橋本次朗, 有木茂, 高宮里奈, 高橋素子, 上原康昭, 長谷川善弘, 高橋聡, 黒木由夫, 舛森直哉

日本泌尿器科学会総会 ( (一社)日本泌尿器科学会総会事務局 ) 103回 742 - 742 2015.04

尿路性器感染症(尿路感染症・性感染症)に用いる薬 (特集これだけは押さえたい泌尿器科でよく使うクスリ : イラストで薬剤の作用機序がわかる!)

桧山佳樹, 髙橋聡

泌尿器ケア ( メディカ出版 ) 20 ( 3 ) 263 - 269 2015.03

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膀胱におけるサーファクタント蛋白質Aの発現と尿路病原性大腸菌に対する感染防御機構

橋本次朗, 高橋聡, 上原康昭, 長谷川喜弘, 舛森直哉, 有木茂, 高宮里奈, 高橋素子, 黒木由雄

日本化学療法学会雑誌 ( (公社)日本化学療法学会 ) 63 ( 2 ) 252 - 252 2015.03

サーファクタント蛋白質による尿路病原性大腸菌感染防御

栗村雄一郎, 上原央久, 橋本次朗, 高橋聡, 舛森直哉, 有木茂, 西谷千明, 高橋素子, 黒木由夫, 塚本泰司

泌尿器外科 ( 医学図書出版(株) ) 26 ( 12 ) 1858 - 1858 2013.12

A CASE OF IgG4-RELATED KIDNEY DISEASE MIMICKING A RENAL CELL CARCINOMA

野藤誓亮, 進藤哲哉, 水野孝祐, 田中俊明, 北村寛, 高橋聡, 舛森直哉, 塚本泰司

泌尿器科紀要 = Acta urologica Japonica ( 泌尿器科紀要刊行会 ) 59 ( 8 ) 513 - 515 2013.08

A 56-year-old man who had a 28mm renal mass on computed tomography (CT) pointed out at another hospital visited our department. The tumor was buried and located in the renal hilus, which was slightly enhanced in the early phase and washed out in the delayed phase. We diagnosed it as papillary renal cell carcinoma and conducted partial nephrectomy. The tumor was ocher and a solid nodule without capsule formation. Although there was no malignancy in histopathological examination, plasma cell infiltration was found and the IgG4/IgG-positive cell ratio was over 40%. Additionally, the serum IgG4 level was elevated postoperatively and the patient was diagnosed as having IgG4-related kidney disease. IgG4-related kidney disease is often found as multiple low-density lesions in CT. Because of the solitary nodule-like formation, it was difficult to distinguish from hypovascular renal cell carcinoma such as the papillary type. When a buried and solitary hypovascular tumor is detected, we must consider IgG4-related kidney disease as a differential diagnosis. Measuring the serum immunoglobulin and complement levels may be helpful for avoiding unnecessary surgery.

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A CASE OF METASTATIC RENAL CELL CARCINOMA WITH NO EVIDENCE OF DISEASE FOR A LONG TERM AFTER A FAVORABLE RESPONSE TO MOLECULAR-TARGETED THERAPY FOLLOWED BY METASTASECTOMY

池端良紀, 高橋聡, 北村寛, 舛森直哉, 塚本泰司

泌尿器科紀要 = Acta urologica Japonica ( 泌尿器科紀要刊行会 ) 59 ( 6 ) 369 - 372 2013.06

We report a case of metastatic renal cell carcinoma (RCC) treated with cytoreductive nephrectomy, cytokine therapy, and molecular targeted therapy followed by metastasectomy. A 47-year-old man was diagnosed as having bilateral RCC with metastases to the lung, liver, left adrenal gland, and lymph nodes in the mediastinum. He initially received right radical nephrectomy, left partial nephrectomy, and left adrenalectomy. The pathological diagnosis was clear cell RCC, which was found in all excised specimens. After the initial surgery, cytokine therapy was started but it did not generate a response in the evaluation at 3 months. He then received sorafenib for 2.5 years and the treatment produced a 75% response rate with only one metastasis remaining. The following sequential therapy with everolimus and sunitinib did not reduce the size of the lung metastasis. For a long time, the metastasis was limited only to the lung and its size did not become larger and there were no new lesions in any organs. Then, lung metastasectomy was performed. From the time of surgery in January 2010 until November 2012, he has had no recurrence of the disease. In some selected cases, sequential therapy with targeted agents followed by metastatectomy may result in a favorable clinical outcome for patients with metastatic RCC.

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A CASE OF SQUAMOUS CELL CARCINOMA OF THE BLADDER IN A PATIENT WITH INTERSTITIAL CYSTITIS

岡田学, 高橋聡, 松木雅裕, 市原浩司, 島正樹, 北村寛, 舛森直哉, 塚本泰司

泌尿器科紀要 = Acta urologica Japonica ( 泌尿器科紀要刊行会 ) 59 ( 1 ) 31 - 33 2013.01

A 60-year-old woman with interstitial cystitis (IC), who had previously received hydrodistention surgery, intravesical instillation of resiniferatoxin and medication, was being followed. Although urinary cytology was regularly tested with no positive findings, computed tomography carried out for screening of recurrent colon cancer showed muscle-invasive squamous cell carcinoma (SCC) of the bladder (cT3bN0M0). Cystectomy was performed, but she died due to rapid disease progression at 3 months postoperatively. Chronic inflammation can be the cause of development of SCC. It is dubious whether the specific treatments for IC affected her disease. In cases of IC with persistent pyuria, the development of SCC should be kept in mind, and affirmative examination including cystoscopy should be done regularly for early detection of the disease.

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サーファクタント蛋白質D(SP-D)は、膀胱上皮への尿路病原性大腸菌感染を防御する膀胱に発現するSP-Dの生理的役割の解明(Surfactant protein D protects the bladder urothelium against uropathogenic Escherichia coli infection: A possible function of SP-D expressed in the bladder)

西谷千明, 栗村雄一郎, 有木茂, 齋藤充史, 長谷川喜弘, 高橋素子, 橋本次朗, 高橋聡, 塚本泰司, 黒木由夫

日本生化学会大会プログラム・講演要旨集 ( (公社)日本生化学会 ) 85回 3P - 843 2012.12

Prediction of Operative and Middle-Term Functional Outcomes after Partial Nephrectomy Using the RENAL Nephrometry Score

K. Ichihara, T. Tanaka, H. Kitamura, S. Takahashi, N. Masumori, T. Tsukamoto

UROLOGY ( ELSEVIER SCIENCE INC ) 80 ( 3 ) S250 - S250 2012.09

Research paper, summary (international conference)

The Frequency of Surgical Site Infections in Radical Retropubic Prostatectomy Is Not Increasing Regardless of Short Duration of Antimicrobial Prophylaxis

U. Teruhisa, T. Satoshi, Kouji, I, T. Toshiaki, K. Hiroshi, M. Naoya, T. Taiji

UROLOGY ( ELSEVIER SCIENCE INC ) 80 ( 3 ) S155 - S156 2012.09

Research paper, summary (international conference)

尿路性器感染症 (創立百周年記念号) -- (わが国における泌尿器科学100年の歴史)

塚本泰司, 高橋聡

日本泌尿器科学会雑誌 ( 日本泌尿器科学会 ) 103 106 - 111 2012.09

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性器伝染性軟属腫 (特集ストップザ性感染症) -- (診断・治療)

高橋聡, 塚本泰司

臨牀と研究 ( 大道学館出版部 ) 89 ( 7 ) 904 - 906 2012.07

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尿路病原性大腸菌感染に対するサーファクタント蛋白質の防御的役割

西谷千明, 栗村雄一郎, 有木茂, 高橋素子, 齋藤充史, 長谷川喜弘, 橋本次朗, 高橋聡, 相馬仁, 塚本泰司, 黒木由夫

日本生化学会大会プログラム・講演要旨集 ( (公社)日本生化学会 ) 84回 4T16a - 5 2011.09

J-GLOBAL

A ROLE FOR PREOPERATIVE SYSTEMIC CHEMOTHERAPY IN NODE-POSITIVE UPPER TRACT UROTHELIAL CARCINOMA TREATED WITH RADICAL NEPHROURETERECTOMY: A RETROSPECTIVE EXPERIENCE

Hiroshi Kitamura, Toshiaki Tanaka, Manabu Igarashi, Naotaka Nishiyama, Fumimasa Fukuta, Tetsuya Shindo, Shin-Ichi Hisasue, Satoshi Takahashi, Naoya Masumori, Taiji Tsukamoto

JOURNAL OF UROLOGY ( ELSEVIER SCIENCE INC ) 185 ( 4 ) E271 - E272 2011.04

Research paper, summary (international conference)

SURFACTANT PROTEINS A AND D INHIBIT UROPATHOGENIC ESCHERICHIA COLI BINDING TO UROTHELIAL CELLS

Yuichiro Kurimura, Teruhisa Uehara, Kohji Ichihara, Jiro Hashimoto, Satoshi Takahashi, Shigeru Ariki, Chiaki Nishitani, Motoko Takahashi, Yoshio Kuroki, Taiji Tsukamoto

JOURNAL OF UROLOGY ( ELSEVIER SCIENCE INC ) 185 ( 4 ) E546 - E546 2011.04

Research paper, summary (international conference)

APP-078 尿路におけるサーファクタント蛋白質(SP-A、SP-D)の感染防御 : 尿路病原性大腸菌の尿路上皮への接着阻害(総会賞応募ポスター,第99回日本泌尿器科学会総会)

栗村雄一郎, 西谷千明, 橋本次朗, 高橋聡, 有木茂, 高橋素子, 黒木由夫, 塚本泰司

日本泌尿器科學會雜誌 ( 社団法人日本泌尿器科学会 ) 102 ( 2 ) 331 - 331 2011.03

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Clinical relevance of single administration of prophylactic antimicrobial agents against febrile events after removal of ureteral stents for patients with urinary diversion or reconstruction

HASHIMOTO Jiro, TAKAHASHI Satoshi, KURIMURA Yuichiro, TAKEYAMA Koh, KUNISHIMA Yasuharu, TSUKAMOTO Taiji

Int J Urol 17 ( 2 ) 163 - 166 2010.02

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Clinical features of patients with brain metastasis from testicular germ cell tumor

松田洋平, 田中俊明, 佐藤俊介, 北村寛, 高橋聡, 舛森直哉, 塚本泰司

Acta urologica Japonica ( 泌尿器科紀要刊行会 ) 56 ( 2 ) 99 - 102 2010.02

We retrospectively reviewed 190 patients with germ cell tumors of testis or extragonadal origin who were treated in our hospital between 1980 and 2007. Five (2.6%) of them had brain metastasis. We studied the clinical features and treatment outcome of these patients. Median age was 30 years old (23-48). The clinical stage was III in all the patients, except one with stage I disease. Two patients had brain metastasis at the initial presentation. All patients underwent multiple regimens of chemotherapy. As local therapies for brain metastases, surgical resection was done in 4 and gamma knife was in 1. Whole brain irradiation was added to surgery in 2 patients. All patients died of the disease within one year after brain metastasis development, except 1 patient who was free of disease 47 months after the presentation. The long survivor had solitary brain metastasis at the initial presentation and received 4 regimens of chemotherapy, 2 surgical resections of brain metastases and whole brain irradiation. Finally, chemotherapy consisting of irinotecan and nedaplatin resulted in normalization of the tumor markers and complete remission was proved by the subsequent surgical resection. Although most patients with brain metastasis have a poor clinical outcome, aggressive local treatment and employment of novel anticancerous agents may contribute to improve clinical course of selected patients with germ cell tumors and brain metastasis.

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Adenomatoid Tumor of the Testis with Testicular Pain : A Case Report

島正樹, 高橋聡, 前田俊浩, 舛森直哉, 伊藤直樹, 塚本泰司

泌尿器科紀要 ( 泌尿器科紀要刊行会 ) 55 ( 5 ) 285 - 286 2009.05

A 30-year-old man who complained of sudden right scrotal pain visited our clinic. Physical examination revealed a palpable mass with tenderness on his right testis. An emergent operation was performed for acute scrotum. The intraoperative findings showed a small, white, elastic solid, a smooth surface tumor that originated from the tunica albuginea of the right testis. It seemed to be benign macroscopically and partial orchiectomy was performed. Soon after surgery, the pain disappeared. Pathohistological examination revealed an adenomatoid tumor of the testis which originated from the rete testis. Adenomatoid tumor of the testis is a rare benign tumor. In general, it can be diagnosed by observation of an incidental, pain-free and small palpable mass of the testis. Therefore, we should be aware that an adenomatoid tumor of the testis can be one of the differential diagnoses of acute scrotum.

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IS CHRONIC PROSTATITIS/CHRONIC PELVIC PAIN SYNDROME ACCOMPANIED WITH SEMINAL VESICULITIS?

R. Furuya, S. Takahashi, S. Furuya, N. Saitoh, H. Ogura, Y. Kurimura, T. Tsukamoto

EUROPEAN UROLOGY SUPPLEMENTS ( ELSEVIER SCIENCE BV ) 8 ( 4 ) 259 - 259 2009.03

Research paper, summary (international conference)

Surgical site infection in patients with radical cystectomy

Satoshi Takahashi, Yuki Kyoda, Naoki Itoh, Taiji Tsukamoto

WOUND REPAIR AND REGENERATION ( WILEY-BLACKWELL PUBLISHING, INC ) 17 ( 1 ) A6 - A6 2009.01

Research paper, summary (international conference)

Condyloma acuminatum

高橋聡, 塚本泰司

Japanese journal of clinical medicine ( 日本臨床社 ) 67 ( 1 ) 153 - 156 2009.01

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Clinical course linkage among different priapism subtypes : Dilemma in the management strategies

HISASUE Shin-ichi, KOBAYASHI Ko, KATO Ryuichi, HASHIMOTO Kohei, YAMASHITA Shinichi, TAKAHASHI Satoshi, MASUMORI Naoya, TSUKAMOTO Taiji

International journal of urology 15 ( 11 ) 1006 - 1010 2008.11

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Gatifloxacin treatment for chronic prostatitis : a prospective multicenter clinical trial

KUNISHIMA Yasuharu, TAKEYAMA Koh, TAKAHASHI Satoshi, MATSUKAWA Masanori, KOROKU Mikio, TANDA Hitoshi, TANAKA Toshiaki, HIROSE Takaoki, IWASAWA Akihiko, NISHIMURA Masahiro, TAKEDA Kou-ichi, SUZUKI Nobukazu, HORITA Hiroki, YOKOO Akifumi, TSUKAMOTO Taiji

Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy 14 ( 2 ) 137 - 140 2008.04

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Current survey of urinary tuberculosis in Hokkaido, Japan

TAKAHASHI Satoshi, TAKEYAMA Koh, KUNISHIMA Yasuharu, HASHIMOTO Kohei, MIYAMOTO Shintaro, ICHIHARA Kohji, TAKAGI Yoshio, TANAKA Toshiaki, HIROSE Takaoki, SUNAOSHI Ken-ichi, TAKEI Fumiyasu, MIYAO Noriomi, KURIMURA Yuichiro, TAKAGI Seiji, TSUKAMOTO Taiji

J Infect Chemother 13 ( 2 ) 105 - 108 2007.04

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Intravesical instillation of resiniferatoxin for the patients with interstitial cystitis

高橋聡, 柳瀬雅裕, 井上隆太, 市原浩司, 舛森直哉, 塚本泰司, 井川靖彦, 西沢理

Acta urologica Japonica ( 泌尿器科紀要刊行会 ) 52 ( 12 ) 911 - 913 2006.12

間質性膀胱炎に対し水圧療法を施行し、その後、症状が再燃した3症例(初診時66歳、59歳、70歳、全例女性)にレジニフェラトキシン膀胱内注入療法を行い、その有効性を検討した。その結果、1)全例、水圧療法時の診断としてNational Institute of Diabetes,Digestive and Kidney Diseasesのクライテリアに合致しており、比較的症状の重い症例であった可能性がみられた。2)1～2回の注入で、特に膀胱部痛に対しは著明に効果があり、1例は5年間、2例は3年間の有効期間で現在に至っている。効果が持続した理由は明らかでない。3)経過観察中、排尿困難等の排尿症状、血液学的異常は認められなかった。しかし一方、排尿量に関しては1回排尿量はやや改善したが、著明な改善は得られなかった。4)膀胱部痛に対しては非常に有効であり、膀胱部痛が主症状である症例を選択できれば、レジニフェラトキシンは間質性膀胱炎に対する治療の1つとして考慮されてよいと考えられた。Although hydrodistention of the bladder is accepted as the initial treatment for patients with interstitial cystitis (IC), second-line treatment for worsening symptoms is not concretely established. Resiniferatoxin (RTX) desensitizes bladder afferent c-fibers and its intravesical instillation is effective for patients with detrusor overactivity. We studied the clinical relevance of intravesical treatment with RTX for patients with IC. The treatment was performed for 3 patients with incomplete improvement after hydrodistention. All 3 patients were free of bladder pain posttreatment and had slight improvement of the maximum voided volume. Though RTX treatment requires general anesthesia against severe bladder pain it is effective for selected patients with interstitial cystitis and can be potentially used as one of the treatment options.

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Analysis of clinical manifestations of male patients with urethritis

TAKAHASHI Satoshi, TAKEYAMA Koh, KUNISHIMA Yasuharu, TAKEDA Kohichi, SUZUKI Nobukazu, NISHIMURA Masahiro, FURUYA Ryoji, TSUKAMOTO Taiji

J. Infect. Chemother. 12 ( 5 ) 283 - 286 2006.10

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Detection of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum DNAs in urine from asymptomatic healthy young Japanese men

TAKAHASHI Satoshi, TAKEYAMA Koh, MIYAMOTO Shintaro, ICHIHARA Kohji, MAEDA Toshihiro, KUNISHIMA Yasuharu, MATSUKAWA Masanori, TSUKAMOTO Taiji

J Infect Chemother. 12 ( 5 ) 269 - 271 2006.10

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Malignant fibrous histiocytoma of the retroperitoneum: review of clinical course and histopathology

市原浩司, 高橋聡, 高橋敦, 舛森直哉, 伊藤直樹, 長谷川匡, 高木良雄, 田口圭介, 塚本泰司

Acta urologica Japonica ( 泌尿器科紀要刊行会 ) 52 ( 10 ) 761 - 764 2006.10

1985年～2005年に診断した悪性線維性組織球腫(MFH;Malignant fibrous histiocytoma)7例(男5例女2例、平均年齢58歳)について検討した。主訴の腹部背部痛は全例の6割で認められ、2例に発熱倦怠感等全身症状を伴った。腫瘍発生部位は後腹膜腔以外に精索周囲組織もあり、腫瘍長径平均11.5cmで、臨床経過は観察期間が中央値5ヵ月、6例で治療目的に腫瘍摘出術を実施した。摘出術実施6例中、術後局所再発は5例、再発までの期間は平均1.5ヵ月であり、手術以外の治療を放射線治療を4例で実施し、うち2例で再発、転移巣の治療を行った。化学治療は1例に実施し、mesna、adriamycin、ifosfamide、dacarbazine(MAID)を用いたが、開始後局所再発で死亡した。死亡した5例の生存期間は平均3.5ヵ月で、現在も生存中の2例中1例が左精索原発のMFHであった。また、1例は右腎被膜由来のMFHで再発を繰り返していたが、その都度完全摘除が可能であった。The definition of malignant fibrous histiocytoma (MFH) was modified and simplified in the new WHO-Soft Tissue and Bone Tumor Classification published in 2002. We reviewed the clinical courses of 7 patients with MFH of the retroperitoneum treated in our hospital from 1985 to 2005 and reexamined their pathological diagnoses according to the new classification. All pathological specimens were reviewed again by an expert pathologist (TH) and were confirmed as MFH. Median follow-up was 5 months (0.5 to 44 months). Five patients were diagnosed as having the pleomorphic type, and 2, the inflammatory type. The tumor was located in the retroperitoneal space in 6 patients and around the left spermatic cord in 1. Radical surgery was the primary treatment for 6 patients. The disease recurred locally in 5 patients and distant metastasis developed in 3. The lung and liver were the principal locations of distant metastases. The 2-year disease-specific survival rate was 30% and 2 patients have been alive for more than 3 years. Our experience and those of others reported in the literature suggested that the factors for long-term survival in MFH are pleomorphic-type histology, superficial location, small tumor size and intensive wide excision, although most patients with MFH might have a poor prognosis. Because the tumor size of MFH tended to be large, especially that in the retroperitoneal cavity, wide excision with sufficient tumor-free margin is hard to guarantee.

CiNii

Hydrodistention of the bladder in patients with interstitial cystitis: clinical efficacy and its association with immunohistochemical findings for bladder tissues

井上隆太, 高橋聡, 砂押研一, 市原浩司, 舛森直哉, 塚本泰司

Acta urologica Japonica ( 泌尿器科紀要刊行会 ) 52 ( 10 ) 765 - 768 2006.10

対象は1996年5月～2005年5月に泌尿器科を受診し、間質性膀胱炎を疑った男4例女23例の27例(平均54.3歳)。間質性膀胱炎の診断は自覚症状と過去の治療歴(抗コリン薬や消炎鎮痛剤による治療で改善なし)に基づいた。尿細胞診にて異型細胞を認めないことを確認し、選択基準である膀胱鏡で点状出血、ハンナー潰瘍、膀胱痛、尿意切迫感を認める点は全例に当てはまった。22例に水圧療法前の膀胱内圧測定が可能で、初発尿意時の膀胱容量は75.2±46.3ml、最大膀胱容量は208.4±121.0mlで、注入中強い膀胱部痛を認め排尿筋活動は認められなかった。potassium sensibility test(PST)は13例に実施し11例が陽性であった。麻酔下膀胱鏡所見はハンナー潰瘍3例、点状出血26例、粘膜亀裂を10例に認めた。水圧療法の合併症で水圧療法中の膀胱破裂と急性腎盂炎が各1例にあり、膀胱破裂は小切開でのドレナージと7日間の尿道カテーテル留置、急性腎盂炎には輸液と抗菌薬投与で軽快した。We retrospectively analyzed the clinical relevance of hydrodistention under anesthesia for patients having urgency and/or lower abdominal pain who were clinically diagnosed as having interstitial cystitis (IC) from May 1996 to May 2005. Their symptoms were refractory to anticholinergic or antiinflammatory agents. Hydrodistention was performed under general or spinal anesthesia with direct vision by cystoscopy and irrigation fluid was instilled into the bladder at a pressure of 80 cmH2O. Cystoscopic findings revealed glomerulation in 26 patients (96%), cracking in 10 (37%) and Hunner's ulcer in 3. Twenty-four patients (89%) obtained improvement of the objective symptoms after treatment. However, symptoms soon deteriorated in 16 patients, and the average duration of efficacy was only 4.7 months (SD; +/-3.7). There were two episodes of complication in this treatment. Bladder rupture occurred during hydrodistention, but was successfully managed with simple percutaneous perivesical drainage. One patient with acute pyelonephritis was treated with an antimicrobial agent without any additional treatment. Although bladder specimens were examined by immunohistochemistry, tryptase and c-kit were not linked with the mast cell count, severity of symptoms or treatment efficacy. Hydrodistention of the bladder may be recommended as the first treatment choice for patients with IC because it provides relatively high efficacy. However, the short duration of the efficacy requires a second-line treatment option for better management of patients with IC.

CiNii

Asymptomatic male HPV infection

高橋聡, 塚本泰司

日本性感染症学会誌 = Japanese archives of sexually transmitted diseases 17 ( 1 ) 47 - 51 2006.06

CiNii

Prostate cell lines secrete IL-8 in response to Mycoplasma hominis through toll-like receptor 2-mediated mechanism

K Takeyama, H Mitsuzawa, T Shimizu, M Konishi, C Nishitani, H Sano, Y Kunishima, M Matsukawa, S Takahashi, K Shibata, T Tsukamoto, Y Kuroki

PROSTATE ( WILEY-LISS ) 66 ( 4 ) 386 - 391 2006.03

BACKGROUND. The roles of Toll-like receptor 2 (TLR2) and Mycoplasma in Prostate inflammation remain unclear. We investigated whether Mycoplasma induced inflammatory cytokine secretion through TLR2-mediated mechanism in prostate cancer cell line. METHODS. Cell surface expression of TLR2 on PC-3 cells were examined by flowcytometry. PC-3 cells were stimulated with Mycoplasina hominis (M. hominis), and IL-8 secretion and NF-kappa B activation were examined. RESULTS. PC-3 cells expressed TLR2 mRNA and cell surface TLR2 protein. The membrane fraction of M. hominis induced IL-8 secretion from PC-3 cells and NF-kappa B activation in a concentration-dependent manner. Transient transfection of the dominant negative mutant TLR2(P681H) into PC-3 cells attenuated M. hominis-induced IL-8 secretion and NF-kappa B activation. Antibody against the extracellular TLR2 domain significantly suppressed M. homnis-induced IL-8 secretion from the prostate cell lines including PC-3, PrEC, and transformed myofibroblasts. CONCLUSIONS. These results clearly demonstrate that the prostate cell line can secrete inflammatory cytokine in response to M. hominis through a TLR2-mediated mechanism.

DOI

Disinfection by antiseptics in management of postoperative surgical wounds in urologic operations

Satoshi Takahashi, Koh Takeyama, Kohei Hashimoto, Yuichiro Kurimura, Megumi Hirobe, Fumiyasu Takei, Ryoji Furuya, Toshiaki Shimizu, Yasuharu Kunishima, Hiroshi Hotta, Masanori Matsukawa, Takaoki Hirose, Taiji Tsukamoto

Acta Urologica Japonica 52 ( 2 ) 89 - 94 2006.02

The objective of this study was to confirm that frequent disinfection by antiseptics is unnecessary on surgical wounds of urologic operations. Patients who received urologic operations were divided into 3 groups that had the same surgical dressings but different frequencies of disinfection and different antiseptics used. Surgical wounds were evaluated clinically and bacteriologically for the period until removal of sutures. Of the 97 patients randomly recruited for the study, 3 developed surgical-site infection (SSI). There was no significant difference in the incidence of SSI among the 3 groups. Bacterial counts of surgical wounds increased over time after operation, to similar extents in the 3 groups. The major isolate was Staphylococcus epidermidis, one of the normal florae on the skin. This strain was found at almost equal frequencies in all groups. In conclusion, our study suggested that covering with the surgical dressing without frequent disinfection by antiseptics was effective for prevention of SSI. Thus, traditional frequent disinfection should be abandoned.

PubMed

Bacterial colonization on intraluminal surface of urethral catheter

M Matsukawa, Y Kunishima, S Takahashi, K Takeyama, T Tsukamoto

UROLOGY ( ELSEVIER SCIENCE INC ) 65 ( 3 ) 440 - 444 2005.03

Objectives. To determine the relationship between catheter-colonizing bacteria and urinary planktonic bacteria. The significance of catheter-colonizing bacteria for the pathogenesis of urinary tract infection remains unclear, although they are involved in the development of catheter-associated urinary tract infection refractory to antimicrobial chemotherapy. Methods. The subjects were 86 inpatients with urethral catheters. Urinary culture and catheter culture were performed simultaneously. The intraluminal catheter surface was swabbed to prepare a suspension. The suspension and a urine specimen from each patient were quantitatively cultured with the dip slide method and the microorganisms identified. Results. The catheters had been mainly placed to monitor urine output after urologic surgery, and their median indwelling period was 3.0 days (range 1 to 35). The overall positive rate of catheter culture was significantly greater than that of urine culture (53.5% and 30.2%, respectively, P < 0.01), even in patients without a recent antibacterial agent history. The difference was observed at day 2 (60% and 13.3%, catheter versus urine culture, respectively, P = 0.011) and days 3 to 6 (52.4% and 14.3%, respectively, P = 0.0 10) of the indwelling period, but was indistinguishable at day 14 and thereafter. The percentage of patients who had the same bacterial species isolated from both specimens increased in a time-dependent manner. Conclusions. The present results revealed considerable differences in the positive rates and bacterial species isolated from urine and catheter cultures, indicating that not all species of bacteria colonizing the intraluminal surface of the urethral catheter are detected as urinary bacteria. These results also suggest that bacterial colonization on the intraluminal catheter surface can precede the emergence of bacteriuria. (c) 2005 Elsevier Inc.

DOI

Surgical antimicrobial prophylaxis in transurethral ureterolithotripsy

Satoshi Takahashi, Koh Takeyama, Shintaro Miyamoto, Yasushi Tanuma, Yoshio Takagi

Journal of Infection and Chemotherapy ( Springer Japan ) 11 ( 5 ) 239 - 243 2005

The aim of this study was to establish a standard protocol for surgical antimicrobial agents for patients who received transurethral ureterolithotripsy (TUL). We retrospectively reviewed the medical charts of patients who received TUL. From October 2002 to December 2003, 2 days (total, four times) of antimicrobial prophylaxis (AMP) was done, and from January 2004 to December 2004, single prophylaxis was done. We analyzed the incidence of postoperative fever and other factors associated with TUL and compared these factors between the 2-day-AMP and single-AMP groups. Of 135 patients with TUL, 66 patients were in the single-AMP group and 69 in the 2-day-AMP group. The incidences of postoperative fever were 4.5% in the single-AMP group and 11.6% in the 2-day-AMP group. No statistically significant difference was found in the incidence of postoperative fever between the two groups. Our study showed that single AMP was effective for patients receiving a TUL operation. In the future, a prospective randomized study will be needed for a large series, with various types of lithotripters being included. © Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases 2005.

DOI PubMed CiNii

Incidence of and risk factors for surgical site infection in patients with radical cystectomy with urinary diversion

Koh Takeyama, Masanori Matsukawa, Yasuharu Kunishima, Satoshi Takahashi, Hiroshi Hotta, Naotaka Nishiyama, Taiji Tsukamoto

Journal of Infection and Chemotherapy ( Springer Japan ) 11 ( 4 ) 177 - 181 2005

In radical cystectomy (RC), surgical-site infection (SSI) remains one of the most frustrating postoperative complications. Only a few reports have assessed SSI after RC according to the guideline authorized by the Centers for Disease Control and Prevention (CDC guideline). In this study, using the guideline, we assessed the incidence, causative organisms, classification, and risk factors for SSI in patients receiving RC with urinary diversion (UD). The subjects of this study were 104 patients who underwent RC with UD at the Department of Urology, Sapporo Medical University Hospital, between January 1996 and December 2003. As prophylactic antimicrobial agents, intravenous cephalosporins or penicillins were started before surgery and maintained until postoperative day 3. Patients who had preoperative bacteriuria were treated with antimicrobial agents before operation to eradicate the bacteria or reduce their number. The CDC guideline was used for the diagnosis of SSI. The overall incidence of SSI was 33%. The most frequent type of SSI was superficial incisional infection (71%). Methicillin-resistant Staphylococcus aureus (MRSA) was the most frequently isolated organism, accounting for 38% of the causative organisms. Operation time was shown to be a significant risk factor for SSI. Preoperative antibacterial chemotherapy for bacteriuria was not sufficiently efficacious to decrease the incidence of SSI. In our study, the incidence of SSI after RC was 33%, although 71% of the infections were superficial. It is necessary to establish more appropriate countermeasures to prevent SSI after RC with UD in particular, that caused by MRSA. © Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases 2005.

DOI PubMed

Clinical relevance of nucleic acid amplification test for patients with urinary tuberculosis during antituberculosis treatment

Satoshi Takahashi, Kohei Hashimoto, Shintaro Miyamoto, Koh Takeyama, Yoshio Takagi, Taiji Tsukamoto

Journal of Infection and Chemotherapy ( Springer Japan ) 11 ( 6 ) 300 - 302 2005

The nucleic acid amplification test (NAAT) has been valuable in the diagnosis of urinary tuberculosis however, no studies have attempted to determine the significance of NAAT post treatment. We encountered three patients with urinary tuberculosis who underwent sequential NAAT during antituberculosis chemotherapy and post treatment. All patients were diagnosed as having urinary tuberculosis by positive NAAT and specific renal deformity revealed by imaging. In two of the three patients, positive culture results were obtained and one was negative in standard culture. During antituberculosis chemotherapy, a negative NAAT was obtained from 3 to 5 months after the start of treatment and no positive culture results were obtained during the same period. At the end of chemotherapy, 6 months or more after the start of medication, all patients had negative NAAT results. These results suggest that NAAT for Mycobacterium tuberculosis provides an effective and rapid detection method for urinary tuberculosis both pre- and post-treatment. © Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases 2005.

DOI PubMed CiNii

A patient with seminal vesiculitis prior to acute chlamydial epididymitis

Ryoji Furuya, Satoshi Takahashi, Seiji Furuya, Koh Takeyama, Taiji Tsukamoto

Journal of Infection and Chemotherapy ( Springer Japan ) 11 ( 5 ) 250 - 252 2005

This is the first report of a case of seminal vesiculitis prior to acute chlamydial epididymitis. At the first visit to the clinic, the patient wished to check whether he had Chlamydia trachomatis in his genital tract, because his wife had been diagnosed as having chlamydial cervicitis. He had no specific symptoms at that time however, transrectal ultrasonograpy (TRUS) revealed swelling of seminal vesicles, which suggested the presence of seminal vesiculitis. Two days after the first visit, he had high-grade fever and was diagnosed as having acute epididymitis caused by C. trachomatis. We had previously reported that seminal vesiculitis was always complicated with acute epididymitis, so this case could provide important evidence that seminal vesiculitis might precede acute epididymitis. It suggested that acute epididymitis could be affected by seminal vesiculitis via the retrograde transmission route. © Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases 2005.

DOI PubMed CiNii

Incidence of sexually transmitted infections in asymptomatic healthy young Japanese men

Satoshi Takahashi, Koh Takeyama, Shintaro Miyamoto, Kohji Ichihara, Toshihiro Maeda, Yasuharu Kunishima, Masanori Matsukawa, Taiji Tsukamoto

Journal of Infection and Chemotherapy ( Springer Japan ) 11 ( 6 ) 270 - 273 2005

The aim of this study was to estimate the incidence rates of asymptomatic sexually transmitted infections (STI) in young men in Sapporo, Japan. A survey of 204 healthy male volunteers was conducted. First-voided urine specimens were used for detection of Chlamydia trachomatis and Neisseria gonorrhoeae with polymerase chain reaction assay, and cotton swab wiping of the external genitalia was used for detection of human papillomavirus (HPV) with the Hybrid Capture method. The incidence rates were 3.4% for C. trachomatis and 5.9% for HPV. No N. gonorrhoeae was detected. In sexually active men who had regular sexual intercourse, the detection rate of C. trachomatis was 4.7% and that of HPV was 8.0%. Our study showed that the incidence rates of asymptomatic STIs were not negligible, and that we should provide screening programs for young men with a high risk for such unrecognized infections and study their natural histories. © Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases 2005.

DOI PubMed CiNii

男性不妊と感染症 (女性診療のための感染症のすべて) -- (その他)

高橋聡, 塚本泰司

産婦人科治療 ( 永井書店 ) 90 813 - 816 2005

CiNii

Systemic inflammatory response syndrome in open versus laparoscopic adrenalectomy

M Mutoh, K Takeyama, N Nishiyama, Y Kunishima, M Matsukawa, S Takahashi, H Hotta, N Itoh, T Tsukamoto

UROLOGY ( ELSEVIER SCIENCE INC ) 64 ( 3 ) 422 - 425 2004.09

Objectives. To compare the surgical invasiveness of laparoscopic adrenalectomy with that of open surgery when the invasiveness is evaluated by the incidence of systemic inflammatory response syndrome (SIRS). Methods. Patients who underwent open adrenalectomy (35 patients) and those who underwent transperitoneal laparoscopic adrenalectomy (35 patients) were included in this study. We retrospectively investigated the incidence and duration of SIRS in the two groups. Of the 70 patients, 39 had primary aldosteronism and 31 had clinically nonfunctioning adrenal tumors. Results. The incidence of SIRS was 51.4% (18 patients) in the open group and 31.4% (1 1 patients) in the laparoscopic group (P = 0.14, Fisher's exact probability test). The mean duration of SIRS was 1.9 days in the open group and 1.2 days in the laparoscopic group (P = 0.04, unpaired t test). Surgical site infection, which occurred in 2 patients in the open group (5.7%) and 3 patients (8.5%) in the laparoscopic group, was the only infectious complication. No patient had any severe or life-threatening complications. Conclusions. Laparoscopic adrenalectomy seems less invasive than open surgery, when the magnitude of the invasiveness is evaluated by the incidence of SIRS.

DOI

Membrane-anchored CD14 is important for induction of interleukin-8 by lipopolysaccharide and peptidoglycan in uroepithelial cells

T Shimizu, S Yokota, S Takahashi, Y Kunishima, K Takeyama, N Masumori, A Takahashi, M Matsukawa, N Itoh, T Tsukamoto, N Fujii

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY ( AMER SOC MICROBIOLOGY ) 11 ( 5 ) 969 - 976 2004.09

We investigated the induction of interleukin-8 (IL-8) by bacterial lipopolysaccharide (LPS) and peptidoglycan (PGN) in the bladder cancer cell lines T24, 5637, UM-UC-3, and HT1197. T24 and 5637 cells strongly induced IL-8 after stimulation with LPS or PGN in a dose- and time-dependent manner, whereas UM-UC-3 and HT1197 cells did so very weakly. The expression of CD14 at the mRNA, total cellular protein, and cell surface protein levels differed among these cell lines, but the expression levels of Toll-like receptors 2 and 4 (TLR2 and TLR4) were not significantly different. The CD14 expression levels were found to correlate with the inducibility of IL-8 by LPS or PGN. Treatment of T24 and 5637 cells with phosphatidylinositol-specific phospholipase C to eliminate CD14 from the cell surface dramatically suppressed the induction of IL-8. On the other hand, UM-UC-3 cells transfected with CD14 cDNA expressed membrane-anchored CD14 and showed more efficent induction of IL-8 by LPS stimulation than untransfected controls. These results suggest that the presence of the membrane-anchored, but not the soluble, form of CD14 is a strong factor in IL-8 induction in bladder epithelial cells in response to bacterial components. The presence of the membrane-anchored form of CD14 may thus be a determinant for the inflammatory response of uroepithelial cells.

DOI

Treatment of men with gonococcal urethritis with clavulanic acid-amoxicillin : effect and pitfalls

TAKEYAMA Koh, TAKAHASHI Satoshi, NISHIMURA Masahiro, KUNISHIMA Yasuharu, MATSUKAWA Masanori, HOTTA Hiroshi, NISHIYAMA Naotaka, TSUKAMOTO Taiji

日本性感染症学会誌 = Japanese archives of sexually transmitted diseases 15 ( 1 ) 112 - 115 2004.06

CiNii

Clinical issue in the evaluation of a cure for male patients with Chlamydia trachomatis urethritis after treatment

TAKAHASHI Satoshi, TAKEYAMA Koh, KUNISHIMA Yasuharu, MATSUKAWA Masanori, NISHIMURA Masahiro, TSUKAMOTO Taiji

日本性感染症学会誌 = Japanese archives of sexually transmitted diseases 15 ( 1 ) 101 - 104 2004.06

CiNii

ハンドアシスト法と pluck technique による腹腔鏡下腎尿管全摘術の臨床的検討

伊藤直樹, 国島康晴, 立木仁, 高橋聡, 松川雅則, 高橋敦, 舛森直哉, 塚本泰司

Japanese journal of endourology and ESWL 17 ( 1 ) 27 - 30 2004.04

CiNii

Is seminal vesiculitis a discrete disease entity? Clinical and microbiological study of seminal vesiculitis in patients with acute epididymitis

R Furuya, S Takahashi, S Furuya, Y Kunishima, K Takeyama, T Tsukamoto

JOURNAL OF UROLOGY ( LIPPINCOTT WILLIAMS & WILKINS ) 171 ( 4 ) 1550 - 1553 2004.04

Purpose: To our knowledge direct evidence of inflammatory involvement of the seminal vesicles has not previously been reported in patients with acute epididymitis. We verified the discrete disease entity of seminal vesiculitis associated with acute epididymitis. Materials and Methods: The study included 13 patients who were clinically diagnosed with acute epididymitis. We report imaging, cytological and bacteriological findings in the seminal vesicles of patients with acute epididymitis. Results: On transrectal ultrasonography 12 of the 13 patients (92.3%) had dilatation of the seminal vesicle on the side ipsilateral to epididymitis. Dilatation of the contralateral side was found in only 4 patients. Seminal vesicle fluid from the ipsilateral side showed inflammatory findings in all patients. In patients 40 years and younger Chlamydia trachomatis was detected in seminal vesicle fluid in 7 of the 8 patients with epididymitis with results positive for the microorganism on first voided urine. Conclusions: Inflammatory responses were found in the seminal vesicles of patients with acute epididymitis. Chlamydia trachomatis was the causative pathogen most frequently detected in seminal vesicle fluid. Seminal vesiculitis is clearly associated with acute epididymitis and it may be a discrete disease entity.

DOI

Antimicrobial agents and suppression of cytokine secretion of prostate cancer cell line

K Takeyama, M Matsukawa, H Hotta, S Takahashi, Y Kunishima, N Nishiyama, Y Kuroki, T Tsukamoto

JOURNAL OF UROLOGY ( LIPPINCOTT WILLIAMS & WILKINS ) 171 ( 4 ) 30 - 30 2004.04

Research paper, summary (international conference)

Incidence of sexually transmitted diseases in Hokkaido, Japan, 1998 to 2001

Satoshi Takahashi, Kou Takeyama, Yasuharu Kunishima, Toshiaki Shimizu, Naotaka Nishiyama, Hiroshi Hotta, Masanori Matsukawa, Masumi Minowa, Takeo Tanihata, Yoshiaki Kumamoto, Taiji Tsukamoto

Journal of Infection and Chemotherapy ( Springer Japan ) 10 ( 3 ) 163 - 167 2004

The objective of this study was to provide precise data on the incidence of sexually transmitted diseases (STDs) in Hokkaido. The goal of this prospective surveillance, study was to clarify the STD incidence between 1998 and 2001 in Hokkaido, Japan. The incidence of gonococcal infection in men was found to be 127-199 per 100000 people per year, which was three or four times higher than that for women. Female genital chlamydial infection had an incidence of 300-400 with a female to male ratio of two or three to one. Younger adults had higher incidences of gonococcal and chlamydial infections than older people. In conclusion, the current study of STDs revealed high incidences of gonococcal and chlamydial infections in the Hokkaido area, and there was no decreasing trend in STD incidence during these 4 years.

DOI PubMed

Prophylactic antimicrobial agents in urologic laparoscopic surgery: 1-Day versus 3-day treatments

Koh Takeyama, Toshiaki Slumizu, Masatoshi Mutoh, Naotaka Nishiyama, Yasuharu Kunishima, Masanori Matsukawa, Satoshi Takahashi, Hiroshi Hotta, Naoki Itoh, Taiji Tsukamoto

Journal of Infection and Chemotherapy ( Springer Japan ) 10 ( 3 ) 168 - 171 2004

Although the incidence of surgical site infection (SSI) is generally low in laparoscopic urologic surgery, the standard protocol for prophylactic use of antimicrobial agents remains to be established. We retrospectively compared the incidence and severity of SSI after laparoscopic surgery between two different protocols for prophylactic use of antimicrobial agents. This study included 114 patients who underwent urologic laparoscopic surgery categorized as "clean" or "clean-contaminated" in Sapporo Medical University School Hospital between January 1996 and October 2002. As a prophylactic antimicrobial agent, one of the cephalosporins or penicillins was administered intravenously to all patients. For 46 consecutive patients between January 1996 and July 2000, an antimicrobial agent was given 30min before operation and thereafter every 12h on the same day and the next 2 days after operation (the 3-day group). For 68 consecutive patients from August 2000 to October 2002, an antimicrobial agent was given once 30 min before operation and was additionally given only in the evening or night of the day of operation (the 1-day group). The incidence of SSI was retrospectively investigated. There were two patients who developed SSI in each group (4.3% in the 3-day group and 2.9% in the 1-day group). The incidence of SSI was not significantly different between the two groups. The 1-day protocol has efficacy equal to that of the 3-day protocol in prophylaxis of SSI. The 1-day use of a prophylactic antimicrobial agent may be recommended for the clean or clean-contaminated urologic laparoscopic surgery described above.

DOI PubMed

Detection of human papillomavirus DNA on the external genitalia of healthy men and male patients with urethritis

S Takahashi, T Shimizu, K Takeyama, Y Kunishima, H Hotta, M Koroku, H Tanda, T Saka, M Nishimura, A Iwasawa, R Furuya, T Hirose, Kobayashi, I, Y Kumamoto, T Tsukamoto

SEXUALLY TRANSMITTED DISEASES ( LIPPINCOTT WILLIAMS & WILKINS ) 30 ( 8 ) 629 - 633 2003.08

Background. Only a few studies have been done involving detection of human papillomavirus (HPV) DNA on the external genitalia of men without genital warts, although many have been done for women. We conducted HPV DNA detection among healthy male volunteers and men with urethritis, both having no visible lesions on their external genitalia. Goal. The goal of the study was to determine the detection rate of HPV DNA in volunteers and patients with urethritis and to determine risk factor(s) for positive DNA. Study Design: This was a prospective clinical study. Results: HPV DNA was found in 1.3% of 75 volunteers and in 18.5% of 130 patients with urethritis. DNA of a high-intermediate oncogenic risk was more predominant than the low-risk type. Among various risk factors, only a history of STD was a significant factor for the positive detection of HPV DNA in multiple regression analysis. Conclusion: HPV DNA was found in patients with urethritis more frequently than in volunteers, probably because the former had higher sexual activity.

DOI

Application of real-time polymerase chain reaction technology to detect prostatic bacteria in patients with chronic prostatitis/chronic pelvic pain syndrome

S Takahashi, DE Riley, JN Krieger

WORLD JOURNAL OF UROLOGY ( SPRINGER-VERLAG ) 21 ( 2 ) 100 - 104 2003.04

To investigate the potential association between prostate infection and chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS), we used molecular approaches described in previous reports. These methods employed standard polymerase chain (PCR) reaction assays to provide a qualitative evaluation of prostatic bacterial species. Here, we report on the detection of prostatic bacteria using a real-time PCR. Template DNAs were examined from prostatic tissue samples from patients with CP/CPPS. Two PCR primer sets were used: one that amplifies a portion of all known bacterial ribosomal DNAs (16S rDNAs) and one that is specific for Escherichia coli as opposed to related, E. coli-like bacteria. The 16S rDNA real-time PCR assay detected bacterial DNAs in eight (26%) of 31 samples from patients with CP/CPPS, including three samples (10%) that were also positive by the E. coli real-time PCR assay. These E. coli positives were quantified at approximately 10(3) cfu/ml of tissue digested. Quantification, speed and specificity make real-time PCR a promising approach for the quantitative detection and identification of prostatic bacteria from CP/CPPS patients.

DOI

Is seminal vesiculitis a disease entity? Its association of acute epididymitis

R Furuya, S Furuya, H Shinichi, T Shimizu, S Takahashi, A Takahashi, N Masumori, N Itoh, T Tsukamoto

JOURNAL OF UROLOGY ( LIPPINCOTT WILLIAMS & WILKINS ) 169 ( 4 ) 30 - 30 2003.04

Research paper, summary (international conference)

Chronic prostatitis: Role of uncommon organisms

JN Krieger, S Takahashi, DE Riley

EUROPEAN UROLOGY SUPPLEMENTS ( ELSEVIER SCIENCE BV ) 2 ( 2 ) 19 - 22 2003.03

DOI

Efficacy of an RNA detection test kit in the diagnosis of genital chlamydial infection

Satoshi Takahashi, Toshiaki Shimizu, Kou Takeyama, Ryouji Furuya, Mikio Koroku, Hitoshi Tanda, Masahiro Nishimura, Akihiko Iwasawa, Hiroshi Yoshio, Naoki Itoh, Taiji Tsukamoto

Journal of Infection and Chemotherapy ( Springer Japan ) 9 ( 1 ) 90 - 92 2003

A nucleic acid amplification method based on DNA detection, the current standard method for the diagnosis of genital infection by Chlamydia trachomatis, has been shown to potentially yield false-positive results after treatment in the clinical setting. RNA detection methods are more appropriate because viable organisms have multiple RNA copies that are surely detected by the method. In this study, we evaluated the efficacy of a new RNA detection test kit, the VIDAS PROBE CT test, in the diagnosis of genital chlamydial infection. For comparison, the standard DNA detection method, Amplicor STD-I, was also used in the study. First voided-urine samples and urethral smears from male patients with urethritis, and first voided-urine samples and cervical smears from female patients with cervicitis served as samples for the detection of C. trachomatis. Of the 60 first voided-urine samples from male patients, 21 were positive and 39 negative with the VIDAS PROBE CT test. Amplicor STD-I achieved exactly the same result. In female patients with cervicitis, the two test kits produced the same result, with 2 positive cervical smears and 38 negative. These results suggest that the VIDAS PROBE CT test is as efficient as Amplicor STD-I in the detection of C. trachomatis. While studies including a greater number of patients will be needed for revealing the unique advantages of the new RNA detection test kit, VIDAS PROBE CT, we concluded from the current study that the test may be clinically useful in the diagnosis of genital chlamydial infection.

DOI PubMed

National Institutes of Health Chronic Prostatitis Symptom Index for Japanese men

Y Kunishima, M Matsukawa, S Takahashi, N Itoh, T Hirose, S Furuya, K Takatsuka, M Mori, T Tsukamoto

UROLOGY ( ELSEVIER SCIENCE INC ) 60 ( 1 ) 74 - 77 2002.07

Objectives. To establish a Japanese version of the National Institutes of Health Chronic Prostatitis Symptom Index (NIH-CPSI) and to determine its validity and applicability in patients with chronic prostatitis, comparing the results with those from patients with benign prostatic hyperplasia (BPH) and normal men. Methods. The study included 103 patients with chronic prostatitis, 60 with BPH, and 87 normal men who agreed to respond to a self-administered questionnaire of the Japanese version of the NIH-CPSI (JPN-NIH-CPSI). Testing-retesting was done to confirm the reliability of the index in 23 patients with chronic prostatitis, with a 2-week interval between tests. Results. Pain or discomfort was more prominent in the prostatitis group than in the BPH and normal groups. Overall, quality of life in the prostatitis group was impaired more severely than that in the other two groups. The scores of three domains (pain, urinary symptoms, and quality-of-life impact) in the prostatitis group were clearly higher than those in the BPH and normal groups, except for those of urinary symptoms in the BPH group. The test-retest analysis showed good reliability and internal consistency for the JPN-NIH-CPSI, with a Cronbach's alpha coefficient of more than 0.8. Conclusions. The JPN-NIH-CPSI can be reliably used for evaluation of Japanese patients with prostatitis, as is the NIH-CPSI for English-speaking men. (C) 2002, Elsevier Science Inc.

DOI

Multidrug-resistant Pseudomonas aeruginosa isolated from the urine of patients with urinary tract infection

K. Takeyama, Y. Kunishima, M. Matsukawa, S. Takahashi, T. Hirose, N. Kobayashi, I. Kobayashi, T. Tsukamoto

Journal of Infection and Chemotherapy ( Springer Japan ) 8 ( 1 ) 59 - 63 2002

We report the clinical courses of 3 patients with urinary obstruction who developed acute pyelonephritis caused by multidrug-resistant (MDR) Pseudomonas aeruginosa. Genome fingerprinting was performed to clarify the route of cross-infection, and an imipenem-resistance gene was detected by the polymerase chain reaction (PCR) method. The study included 17 patients at our institute who had urinary tract infections caused by P. aeruginosa between January and December 1997. MDR was defined as that when all the minimum inhibitory concentrations (MICs) were determined to show resistance according to the breakpoints recommended by the National Committee for Clinical Laboratory Standards (NCCLS) for P. aeruginosa. Pulse-field gel electrophoresis (PFGE) was carried out for genome fingerprinting. PCR was used to detect the metallo-β-lactamase gene (blaIMP). Three strains were revealed for MDR. The strains were isolated from the 3 patients with urinary tract obstruction who developed acute pyelonephritis. The treatment consisted of urinary drainage for the obstructed urinary tract and parenterally administered antimicrobials. Although none of the strains was susceptible to any antimicrobials, all patients had favorable outcomes. PFGE revealed that two strains had an identical genotype, implying cross-infection between the patients. The blaIMP gene was not detected in any of the three strains. In febrile patients with urinary tract infection caused by MDR P. aeruginosa, treatment for urinary obstruction is strongly recommended. Initial empirical chemotherapy with antimicrobials to which the organism is not susceptible is often inevitable. Because there was epidemiological evidence of cross-infection with MDR P. aeruginosa, countermeasures against nosocominal infection are warranted.

DOI PubMed

Complications of Stamey needle suspension for female stress urinary incontinence

S Takahashi, N Miyao, T Hisataki, M Matsukawa, S Takagi, T Sasao, H Adachi, M Miyake, T Tsukamoto

UROLOGIA INTERNATIONALIS ( KARGER ) 68 ( 3 ) 148 - 151 2002

Introduction: The Stamey bladder neck suspension for stress urinary incontinence in females is thought to be an excellent procedure. However, recent studies revealed that complications of this procedure have not been negligible. In this retrospective study, complications of the Stamey needle bladder neck suspension were examined. Materials and Methods: The Stamey procedure was performed either alone or in combination with anterior colporrhaphy for 86 female patients with stress urinary incontinence between 1989 and 1999. The mean follow-up period was 37.6 months and the mean age was 59.1 years. We studied the complications postoperatively pointed out by patients' complaint and image examination. Results: The overall incidence of complications was 37.2%. Voiding difficulties and lower abdominal pain were present in 15 cases, respectively. We experienced 2 unusual cases in whom suspensory stitches had to be removed due to abdominal pain. Conclusions: Although the Stamey bladder neck suspension is thought to be an excellent procedure for stress urinary incontinence, surgeons should beware of possible complications and be alert of the symptoms, such as abdominal pain and bladder irritation. Copyright (C) 2002 S. Karger AG, Basel.

DOI

Epidemiological Survey of Sexually Transmitted Diseases Prevalence in Hokkaido

NISHIYAMA Naotaka, TAKAHASHI Satoshi, FURUYA Ryouji, TAKEYAMA Koh, SHIMIZU Toshiaki, KUNISHIMA Yasuharu, MATSUKAWA Masanori, KUMAMOTO Yoshiaki, MINOWA Masumi, TSUKAMOTO Taiji

日本性感染症学会誌 = Japanese archives of sexually transmitted diseases 12 ( 1 ) 117 - 122 2001.07

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Staphylococcus aureus bacteriuria and surgical site infections by methicillin-resistant Staphylococcus aureus

M Matsukawa, Y Kunishima, S Takahashi, K Takeyama, T Tsukamoto

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS ( ELSEVIER SCIENCE BV ) 17 ( 4 ) 327 - 330 2001.04

Surgical site infection (SSI) remains an important cause of morbidity among hospitalized patients. We reviewed 421 patients who underwent open urological operations between January 1993 and December 1997 in our institute. Group I consisted of 259 patients who received uncontrolled antimicrobial prophylaxis (AMP) between 1993 and 1995. Group II consisted of 162 patients who received controlled AMP between 1996 and 1997. In group II, penicillins or first to second-generation cephalosporins was used and the duration of use for these agents regulated according to the wound class of each operation. The operations with clean wounds showed the lowest rate of SSI in both groups; the operations with contaminated wounds showed the highest rate of SSI (32.0% in group I and 33.3%, in group II). There was no significant difference in the total rates of SSI between the two groups (P = 0.216). The most frequently isolated bacterial species was methicillin-resistant Staphylococcus aureus (MRSA), isolated in 73.3% of the cases in group I and in 93.3%, in group II. There was no significant difference in the incidence of MRSA isolation between the two groups (P = 0.114). The controlled AMP could not lower the incidence of MRSA-induced SSls. In SSI patients, 22.7% of group I and 35.7% in group II. had MRSA bacteriuria before operation, The prohibition of third-generation cephalosporins and shorter duration of AMP did not reduce the incidence of SSI induced by MRSA because MRSA was not the emerging microorganism but rather a resident in the urological ward. On the other hand, the total incidence of SSI did not increase after regulation of AMP. This finding suggests that older antibacterial agents can prevent infection, except those caused by resistant microorganisms such as MRSA. The effective counter-measure for the prevention of MRSA-induced SSI is needed. (C) 2001 Elsevier Science B.V. and International Society of Chemotherapy. All rights reserved.

DOI

Efficacy of a 14-day course of oral ciprofloxacin therapy for acute uncomplicated pyelonephritis

S. Takahashi, T. Hirose, T. Satoh, R. Kato, S. Hisasue, S. Takagi, T. Shimizu, Y. Kunishima, M. Matsukawa, N. Itoh, T. Tsukamoto

Journal of Infection and Chemotherapy ( Springer Japan ) 7 ( 4 ) 255 - 257 2001

This study aimed to evaluate the efficacy and safety of oral antibacterial treatment with fluoroquinilone for acute uncomplicated pyelonephritis Thirteen female patients with acute uncomplicated pyelonephritis were treated with oral fluoroquinilone (ciprofloxacin CPFX). They received 200mg of the drug three times a day while febrile (3-5 days). Once they become afebrile, the same dose of the drug, given twice a day, was given for another 9-11 days. The mean duration of the course of CPFX was 14 days. Symptoms were evaluated, and laboratory examinations, including urine culture and measurement of the minimal inhibitory concentration (MIC) of CPFX were conducted before treatment, and 3, 7, 14, 21, and/or 28 days after the initiation of the treatment. Of the 13 patients, only 5 needed to be hospitalized, and the period of hospitalization was only a few days. On the 14th day after the commencement of treatment, bacteriologic and clinical cure rates were 100%. Escherichia coli was the most common uropathogen, being isolated from the urine of 8 patients. No clinical relapse of the disease was found during a follow-up period of up to 4 weeks. The patients tolerated the drug well without developing any serious adverse effects. Oral antimicrobial chemotherapy with fluoroquinolone, given on an outpatient or short-term hospitalization basis, achieved satisfactory bacteriological and clinical outcomes in the treatment of acute uncomplicated pyelonephritis. This treatment regimen is indicated for patients with this disease who are not in a serious condition with complications such as shock.

DOI PubMed CiNii

Efficacy of prophylactic single intravenous administration of antimicrobials for patients receiving systematic transrectal prostate biopsy

Hiroshi Hotta, Masanori Matsukawa, Yasuharu Kunishima, Naotaka Nishiyama, Toshiaki Shimizu, Satoshi Takahashi, Taiji Tsukamoto

Japanese Journal of Chemotherapy 49 ( 11 ) 642 - 644 2001

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Efficacy of prophylactic single intravenous administration of antimicrobials for patients receiving systematic transrectal prostate biopsy

HOTTA Hiroshi, MATSUKAWA Masanori, KUNISHIMA Yasuharu, NISHIYAMA Naotaka, SHIMIZU Toshiaki, TAKAHASHI Satoshi, TSUKAMOTO Taiji

日本化学療法学会雑誌 49 ( 11 ) 642 - 644 2001

DOI CiNii

Comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (1999) I. Susceptibility distribution

Y. Kumamoto, T. Tsukamoto, T. Hirose, M. Matsukawa, S. Takahashi, Y. Kunishima, M. Ogihara, K. Ishibashi, S. Shigeta, H. Yoshida, Y. Imafuku, M. Murai, K. Watanabe, Y. Kobayashi, H. Uchida, S. Matsuda, S. Sato, M. Fujime, K. Fujita, J. Igari, T. Oguri, K. Yamaguchi, T. Matsumoto, F. Kashitani, H. Ooe, M. Nishikawa, T. Oka, M. Kitamura, Y. Takano, Y. Matsuoka, T. Furuhama, H. Kumon, K. Monden, S. Kohno, Y. Miyazaki, K. Izumikawa, T. Yamaguchi, C. Mochida

Japanese Journal of Antibiotics 54 ( 5 ) 185 - 216 2001

The bacterial strains isolated from patients diagnosed as having urinary tract infections (UTIs) in 9 institutions in Japan were supplied between the period of August 1999 to July 2000. Then, the susceptibilities of them to many kinds of antimicrobial agents were investigated. The number of them were 499 strains. The breakdown of these strains was Gram-positive bacteria as 31.3% and Gram-negative bacteria as 68.7%. Susceptibilities of these bacteria to antimicrobial agents were as follows vancomycin (VCM), ampicillin (ABPC) and imipenem (IPM) showed strong activities against Enterococcus faecalis. The increase of low-susceptible strains which was noticed in the former year showed a slight recovery in this year. VCM showed a strong activity against MRSA preventing growth of all strains with 1 μg/ml. In addition, the activity of arbekacin (ABK) was also strong with the MIC90 of 2 μg/ml against MRSA. However, MSSA and MRSA showing low susceptibilities were detected in one strain each (MIC: 16 μg/ml and 32 μg/ml, respectively). Carbapenems showed high activities against Citrobacter freundii and Escherichia coli. Meropenem (MEPM) prevented growth of all strains within 0.125 μg/ml. Quinolone resistant E. coli decreased in this year compared with those in the last year, that percentage was less than 5%. Almost all drugs showed strong activities against Klebsiella pneumoniae and Proteus mirabilis. MEPM and carumonam (CRMN) prevented growth of all strains within 0.125 μg/ml. On the other hand, one strain of K. pneumoniae showing resistance to cefaclor (CCL) and one strain of P mirabilis showing low susceptibility to most of cephems were detected. Against Pseudomonas aeruginosa, almost drugs were not so active. The MIC90s of carbapenems were 8 μg/ml and those of all other drugs were more than 16 μg/ml.

DOI PubMed

In vitro analysis of the change in resistance of Chlamydia trachomatis under exposure to sub-MIC levofloxacin for a therapeutic term

S Takahashi, T Hagiwara, S Shiga, T Hirose, T Tsukamoto

CHEMOTHERAPY ( KARGER ) 46 ( 6 ) 402 - 407 2000.11

Background: While fluoroquinolone-resistant Chlamydia trachomatis strains have not been clinically isolated, they were isolated in an in vitro study recently. Methods: To determine whether C, trachomatis strains develop resistance under sub-MIG antibacterial exposure in a clinical therapeutic term, C, trachomatis strains were exposed to sub-MIG levofloxacin (LVFX) for about 2 weeks. The MIG of LVFX was measured and DNA fingerprinting was performed every 72 h by PCR using random primers. Results: There was almost no change in the MIG under exposure to 0.125 mug/ml LVFX. However, some mutation-al changes in DNA fingerprints developed. Conclusions: In clinical therapeutic terms, resistant strains of C, trachomatis will probably not develop, even if sub-MIG LVFX is employed, Copyright (C) 2000 S. Karger AG, Basel.

DOI PubMed CiNii

Changes in susceptibility of Pseudomonas aeruginosa to gatifloxacin and carbapenem in an in vitro urinary bladder model

S Takahashi, T Hirose, M Sano, M Nishimura, M Matsukawa, M Mikami, T Tsukamoto

CHEMOTHERAPY ( KARGER ) 46 ( 3 ) 204 - 208 2000.05

To study the changes of bacterial susceptibility during exposure to antimicrobials, the respective minimal inhibitory concentrations (MICs) of 50 colonies from each of various bacterial test strains were determined in an experimental system using an in vitro urinary bladder model. The MICs of gatifloxacin and meropenem for Pseudomonas aeruginosa strains were not homogeneous before exposure to the drugs. The MICs of the strains examined reflected only that of their dominant population. When starting from 10(7) CFU/ml, the bacterial count was determined serially during exposure to gatifloxacin or meropenem in therapeutic urinary concentrations. The MIC of the less susceptible population of each strain was changed serially to a higher one. The more susceptible strains were eradicated before the change of the MIC. The bacteria originally consisted of strains having heterogeneous susceptibility to drugs, in which major strains had susceptibility expressed by the MIC and minor ones had a higher or lower MIC. This heterogeneity may be responsible for the change of susceptibility in strains after exposure to the drugs. Copyright (C) 2000 S. Karger AG, Basel.

DOI PubMed CiNii

Bactericidal activity of gatifloxacin (AM-1155) against Pseudomonas aeruginosa and Enterococcus faecalis in an in vitro bladder model simulating human urinary concentrations after oral administration

S Takahashi, H Ebisu, T Hirose, M Sano, M Nishimura, K Hirai, T Tsukamoto, M Hosaka

CHEMOTHERAPY ( KARGER ) 46 ( 2 ) 122 - 128 2000.03

The bactericidal activity of gatifloxacin, a new 6-fluoro-8-methoxy quinolone, was determined in a dynamic in vitro model mimicking complicated lower urinary tract infection. Strains of Pseudomonas aeruginosa and Enterococcus faecalis with different susceptibility were exposed to changing gatifloxacin concentrations, simulating human urinary concentrations afer oral treatment with 200 mg twice daily for 3 consecutive days. Bacterial numbers of P. aeruginosa (minimal inhibitory concentrations, MIG: less than or equal to 32 mu g/ml) and of E. faecalis (MIC: 16 mu g/ml) were reduced to undetectable levels during exposure. For the strains with lower susceptibility, gatifloxacin showed bactericidal activity, but eradication was not complete. Thus, in a complicated urinary tract infection model, breakpoint MICs of gatifloxacin for uropathogenic organisms were presumed to range from 16 to 32 mu g/ml. At least 86% of recent clinical isolates of P. aeruginosa and E. faecalis were inhibited at its breakpoint MIG. These results suggest that gatifloxacin may be useful in the treatment of urinary tract infections. Copyright (C) 2000 S.Karger AG, Basel.

DOI PubMed CiNii

Detection of antimicrobial-treated Chlamydia trachomatis with Amplicor PCR test kit

S. Takahashi, T. Hagiwara, S. Shiga, T. Hirose, T. Tsukamoto

Journal of Infection and Chemotherapy ( Springer Japan ) 6 ( 4 ) 211 - 215 2000

A polymerase chain reaction (PCR) method for the detection of Chlamydia trachomatis has been developed and is now available in the clinical setting. However, one of the major problems with this method is possible false-positive detection posttreatment. Nonviable C. trachomatis was established by in-vitro exposure to an antimicrobial agent, and we tried to detect the nonviable cells (NVCs) of C. trachomatis by PCR with variant primer sets. C. trachomatis strains (D/UW-3/Cx) were cultured in a medium containing the antimicrobial agent, at 8×MIC (minimal inhibitory concentration) 15 to 20h postinfection. Amplicor and two sets of PCR primers were used to detect the DNA of NVCs. Serial passages of NVCs were done five times. All samples were positive on Amplicor, and all except the fourth passage were positive for the two sets of primers. Although the PCR test appears to be valuable, NVCs may possibly be detected by this method, and this may be clinically responsible for the false detection of C. trachomatis after appropriate antimicrobial chemotherapy.

DOI PubMed CiNii

Biofilm in complicated urinary tract infection

T Tsukamoto, M Matsukawa, M Sano, S Takahashi, H Hotta, N Itoh, T Hirose, Y Kumamoto

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS ( ELSEVIER SCIENCE BV ) 11 ( 3-4 ) 233 - 236 1999.05

We studied the clinical efficacy of oral treatment with ciprofloxacin (CPFX) alone and combined with clarithromycin (CAM) in patients with complicated urinary tract infection with or without an indwelling catheter. Patients were randomly allocated to 600 mg CPFX (CPFX group) or to 600 mg CPFX plus 600 mg CAM (combination group) for 14 days. Evaluation was done on day 14 according to the criteria advocated by the Japanese Urinary Tract Infection Committee. In patients with a urinary catheter, the combination achieved a higher complete bacterial elimination rate (50.0%) and clinical efficacy rate (83.9%) than CPFX alone (30.0 and 61.5%, respectively). While no significant difference was found in the bacterial elimination rate between the two groups, the clinical efficacy of the combination (40.0%) was superior to that of CPFX alone (23.3%) in patients with an indwelling catheter. The better clinical efficacy of the combination may partly be attributed to the antibiofilm effect of CAM in the clinical setting. The results also indicate that difficulties still remain in the treatment of complicated urinary tract infections in patients with an indwelling catheter. (C) 1999 Elsevier Science B.V. All rights reserved.

DOI PubMed CiNii

Inhibitory action of clarithromycin on glycocalyx produced by MRSA

Masato Sano, Takaoki Hirose, Masahiro Nishimura, Satoshi Takahashi, Masanori Matsukawa, Taiji Tsukamoto

Journal of Infection and Chemotherapy ( Springer Japan ) 5 ( 1 ) 10 - 15 1999

We determined whether clarithromycin (CAM) had the ability to eliminate glycocalyx and biofilm produced by methicillin-resistant Staphylococcus aureus (MRSA) using an in-vitro experimental system (consisting of a bladder model and a kidney model) simulating complicated urinary tract infection (UTI). We also examined whether a combination of CAM and vancomycin (VCM) was effective for eliminating the MRSA biofilm. VCM (urinary concentration simulating drip infusion of 500 mg twice a day for 5 days minimum inhibitory concentration (MIC) 0.5 μg/ml) eliminated MRSA from the bladder model medium at 48h, but reproliferation occurred immediately after withdrawal of the agent. No disappearance of MRSA biofilm was noted, and this appeared to be the cause of the bacterial regrowth. CAM (urinary concentration simulating oral administration of 200 mg twice a day for 5 days MIC, 128 μg/ml) allowed microbial recovery to the initial level within 48 h, but led to the disappearance of the glycocalyx-forming biofilm. A combination of VCM and CAM caused microbial elimination from the bladder model medium at 46h with no regrowth after withdrawal of the antimicrobial agents. Scanning electron microscopy confirmed that the MRSA biofilm disappeared completely and no microbial adhesion was noted. These results suggest that CAM has an inhibitory action on glycocalyx and biofilm of MRSA, and that the combined use of VCM and CAM may be efficacious for the treatment of MRSA UTI.

DOI PubMed CiNii

Analysis of cross infection using genomic fingerprinting in nosocomial urinary tract infection caused by Enterococcus faecalis

Satoshi Takahashi, Takaoki Hirose, Masanori Matsukawa, Toshiaki Shimizu, Yasuharu Kunishima, Koh Takeyama, Akifumi Yokoo, Hiroshi Hotta, Midori Mikami, Taiji Tsukamoto

Journal of Infection and Chemotherapy ( Springer Japan ) 5 ( 1 ) 46 - 48 1999

To evaluate cross infection and a possible outbreak of Enterococcus faecalis urinary tract infection (UTI) in our urology ward, we studied the DNA fingerprinting of E. faecalis strains isolated from nosocomial UTI patients, in the period 1982-1996, using arbitrarily primed polymerase chain reaction (AP-PCR) analysis. The serovar and amplified products of DNA extracted from clinically isolated urinary E. faecalis strains by the AP-PCR method were analyzed, and the respective isolation periods of E. faecalis- positive UTI patients were investigated. There were nine patients with E. faecalis UTI between March and May 1994 and all strains isolated from their urine specimens were serovar type 7. AP-PCR revealed that five of the nine isolates had the same pattern. It appeared that these strains had caused the outbreak of E. faecalis UTI. Cross-infection between patients with E. faecalis UTI was demonstrated by genomic fingerprinting, suggesting that cross infection had occurred via urinary catheters or by hand contact in our ward. We may, therefore, reasonably conclude that we should beware of the transmission of urinary E. faecalis and take counter-measures against its dissemination.

DOI PubMed CiNii

Bactericidal effect of levofloxacin on strains with equal susceptibility in an in vitro urinary bladder model

S Takahashi, M Sano, M Nishimura, M Matsukawa, M Mikami, T Hirose, T Tsukamoto

CHEMOTHERAPY ( KARGER ) 44 ( 5 ) 337 - 342 1998.09

The in vitro bactericidal effects of therapeutic concentrations of levofloxacin (LVFX) on clinical isolates of Pseudomonas aeruginosa and Enterococcus faecalis were assessed, Starting from 10(7) CFU/ml, the bacterial count was determined serially during the exposure to LVFX present at concentrations automatically simulated to its clinically achievable urinary levels at the recommended dosage (100 mg 3 times daily), An isolate of P. aeruginosa for which the MIC of LVFX was 16 mu g/ml was completely eradicated after 10 h of exposure to LVFX, On the other hand, 40 h of exposure to LVFX were required for complete eradication of an isolate of E. faecalis with equal susceptibility, although the bacterial count was rapidly reduced to 10(2) CFU/ml in the early phase of the exposure, It was concluded that LVFX exerts a more potent bactericidal effect on P. aeruginosa than on E, faecalis despite a similar susceptibility of these organisms to LVFX.

DOI PubMed CiNii

Follow up of urology patients discharged with urinary methicillin-resistant Staphylococcus aureus

S Takahashi, T Hirose, K Takeyama, T Satoh, T Tsukamoto

JOURNAL OF HOSPITAL INFECTION ( W B SAUNDERS CO LTD ) 37 ( 3 ) 249 - 251 1997.11

Rapid communication, short report, research note, etc. (scientific journal)

A CLINICAL STUDY ON COMBINATION THERAPY OF ANTIMICROBIAL AGENTS FOR COMPLICATED URINARY TRACT INFECTION -With Special Reference to Combination with Clarithromycin-

SANO Masato, TAKAHASHI Satoshi, NISHIMURA Masahiro, HIROSE Takaoki, KUMAMOTO Yoshiaki, TSUKAMOTO Taiji, KOBAYASHI Yorimichi

The Japanese Journal of Urology ( The Japanese Urological Association ) 88 ( 6 ) 596 - 604 1997

(Purpose) To confirm the clinical efficacy of the combined therapy to complicated urinary tract infection (UTI), we conducted a comparative clinical study of the combined therapy with ciprofloxacin (CPFX) and clarithromycin (CAM) acting an biofilm elimination or CPFX alone in patients with complicated UTI.
(Patients and Methods) The study was carried out in patients with complicated UTI having WBCs with 5/hpf or more in urinary sediment and bacteriuria at least 10⁴CFU/ml. The combined therapy was CPFX and CAM, each 600mg/day, for 14 days, and the single therapy group CPFX, 600mg/day, for 14 days. On Day 7 and 14, the eradication rate and efficacy rate (according to the criteria of the Japanese UTI committee) were determined. In the patients with indwelling catheter, the surface of the catheter tip was observed under a scanning electron microscope (SEM) on Day 14.
(Results) In both cases with and without catheters, clinical efficacy was higher in the combined therapy group than in the single therapy group. In paricular, the efficacy rates at 14 Day were significantly higher in the former group. Furthermore, we investigated the theraupetic effect in the below MIC breakpoint of CPFX in complicated UTI. The combined therapy group showed a higher clinical efficacy in both cases with and without indwelling catheter than the single therapy group, although there was not statistically significant. Biofilm on the surface of the catheter tip was eliminated in 75% of the combined therapy group. However, none of the biofilm was eliminated in the single therapy group.
(Conclusion) From the above results, we surmise that the combined use of CPFX and CAM will show some degree of efficacy in eliminating both the causative organism and its biofilm in the complicated UTI.

DOI CiNii

前立腺癌の臨床的検討 : 第317回北海道地方会

笹村啓人, 堀田裕, 舛森直哉, 塚本泰司, 熊本悦明, 高木良雄, 高木誠次, 丸田浩, 西中一幸, 田中吉則, 田中紀明, 林謙治, 門野雅夫, 田口圭介, 横尾彰文, 高塚慶次, 高橋聡, 梅原次男, 三宅正文, 尾田寿朗, 青木正治, 佐藤隆志, 岩沢晶彦, 国島康晴

日本泌尿器科學會雜誌 ( 社団法人日本泌尿器科学会 ) 85 ( 9 ) 1410 - 1410 1994.09

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LEYDIG-CELL TUMOR - A CASE-REPORT WITH REFERENCE TO ITS ENDOCRINOLOGIC FEATURES

N MASUMORI, Y KUMAMOTO, N ITOH, T TSUKAMOTO, N MIYAO, M KOROKU, T HIROSE, S TAKAHASHI

EUROPEAN UROLOGY ( KARGER ) 24 ( 2 ) 302 - 304 1993

Article, review, commentary, editorial, etc. (scientific journal)

We reported an adult case of Leydig cell tumor, which demonstrated an increase of serum estradiol (E2) and suppression of serum testosterone (T) before orchiectomy. The direct inhibitory action of the elevated E2 on the enzymes involved in steroidogenesis and the negative feedback action on luteinizing hormone (LH) secretion of the E2 possibly caused the suppression of serum T. A human chorionic gonadotropin test before the operation revealed the elevation of T and E2, although the response was poor compared with that in normal subjects. However, the postoperative test showed no elevation of these hormones. This result may indicate that the Leydig cell tumor preserves a response to LH stimulation.

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