Research Experience 【 display / non-display 】

Research Experience 【 display / non-display 】

• 2009

  -

  2011

  Sapporo Medical University   School of Medicine   講師

  講師

Research Areas 【 display / non-display 】

Research Areas 【 display / non-display 】

• Life sciences   Obstetrics and gynecology  

Research Interests 【 display / non-display 】

Research Interests 【 display / non-display 】

• ギャップ結合

• 子宮内膜

• パイスタンダー効果

• コネキシン

• ヘルペスウイルス

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Papers 【 display / non-display 】

Papers 【 display / non-display 】

• Analysis of Treatment Response With Proteins Related to Tumor Immunity in Postoperative Irradiated Cervical Cancer Patients.

  Shoh Mafune, Masanori Someya, Tomokazu Hasegawa, Takaaki Tsuchiya, Mio Kitagawa, Toshio Gocho, Ryu Okuda, Masahiro Iwasaki, Motoki Matsuura, Terufumi Kubo, Yoshihiko Hirohashi, Toshihiko Torigoe, Tsuyoshi Saito, Koh-Ichi Sakata

  Anticancer research   44 ( 7 ) 3077 - 3086  2024.07  [International journal]

  　View Summary

  BACKGROUND/AIM: This study evaluated the association between programmed cell death-ligand 1 (PD-L1) and prognosis in patients with cervical cancer treated with postoperative radiation and the impact of neoadjuvant chemotherapy (NAC) on this association. PATIENTS AND METHODS: Immunohistochemical analysis was performed on biopsy specimens from 42 patients who did not receive NAC and from paired samples before (biopsies) and after (resected tissues) chemotherapy from 46 patients who received NAC to determine the association of PD-L1 with radiotherapy outcomes. RESULTS: In the non-NAC group, patients with ≥10% PD-L1-expressing tumor cells prior to treatment had better recurrence-free survival (RFS) than those with <10% PD-L1-expressing tumor cells (p=0.001). In the NAC group, RFS was significantly lower (p=0.005) in the group with a ≥5% reduction of PD-L1 expression in tumor cells after chemotherapy than in those with <5% reduction. In multivariate analysis, only PD-L1 expression (non-NAC group) and the change in PD-L1 expression (NAC group) were associated with RFS. CONCLUSION: Low PD-L1 expression in a cervical tumor prior to treatment was identified as a risk factor for a poor outcome after postoperative radiotherapy. Furthermore, NAC induces an immunological shift that reduces PD-L1 levels in tumor cells, thereby negatively impacting treatment outcomes.

  DOI PubMed

• Prediction of Mismatch Repair Status in Endometrial Cancer from Histological Slide Images Using Various Deep Learning-Based Algorithms.

  Mina Umemoto, Tasuku Mariya, Yuta Nambu, Mai Nagata, Toshihiro Horimai, Shintaro Sugita, Takayuki Kanaseki, Yuka Takenaka, Shota Shinkai, Motoki Matsuura, Masahiro Iwasaki, Yoshihiko Hirohashi, Tadashi Hasegawa, Toshihiko Torigoe, Yuichi Fujino, Tsuyoshi Saito

  Cancers   16 ( 10 )  2024.05  [International journal]

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  The application of deep learning algorithms to predict the molecular profiles of various cancers from digital images of hematoxylin and eosin (H&E)-stained slides has been reported in recent years, mainly for gastric and colon cancers. In this study, we investigated the potential use of H&E-stained endometrial cancer slide images to predict the associated mismatch repair (MMR) status. H&E-stained slide images were collected from 127 cases of the primary lesion of endometrial cancer. After digitization using a Nanozoomer virtual slide scanner (Hamamatsu Photonics), we segmented the scanned images into 5397 tiles of 512 × 512 pixels. The MMR proteins (PMS2, MSH6) were immunohistochemically stained, classified into MMR proficient/deficient, and annotated for each case and tile. We trained several neural networks, including convolutional and attention-based networks, using tiles annotated with the MMR status. Among the tested networks, ResNet50 exhibited the highest area under the receiver operating characteristic curve (AUROC) of 0.91 for predicting the MMR status. The constructed prediction algorithm may be applicable to other molecular profiles and useful for pre-screening before implementing other, more costly genetic profiling tests.

  DOI PubMed

• 経過観察中に自然軽快した広汎性卵巣浮腫の2症例

  馬場 剛, 遠藤 俊明, 新開 翔太, 真里谷 奨, 染谷 真行, 森下 美幸, 岩崎 雅宏, 石岡 伸一, 齋藤 豪

  日本産科婦人科学会雑誌 ( (公社)日本産科婦人科学会 )  76 ( 臨増 ) S - 383  2024.02

• 婦人科内視鏡技術認定医が不在である当院でのTLHの取り組み

  西森 貢隆, 嶋田 浩志, 藤部 佑哉, 鈴木 美紀, 玉手 雅人, 松浦 基樹, 岩崎 雅宏, 齋藤 豪

  北海道産科婦人科学会会誌 ( 北海道産科婦人科学会 )  67 ( 1 ) 91 - 91  2023.12

• Identification of cancer driver mutations in liquid-based cytology samples for the screening of endometrial diseases

  Motoki Matsuura, Kiyoko Takane, Kiyoshi Yamaguchi, Tsuneo Ikenoue, Seira Hatakeyama, Shoko Kurokawa, Masato Tamate, Taishi Akimoto, Masahiro Iwasaki, Shintaro Sugita, Tadashi Hasegawa, Yasunori Ota, Tsuyoshi Saito, Yoichi Furukawa

  BJC Reports ( Springer Science and Business Media LLC )  1 ( 1 )  2023.11

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  Abstract Background Endometrial cancer (EC) is one of the leading causes of cancer death among women and early detection is crucial for its successful treatment. We previously showed that cytological examination combined with genetic analysis using liquid-based cytology (LBC) samples improved the diagnostic sensitivity of EC. Methods Among 218 individuals who underwent endometrial screening by LBC, 208 samples were analysed by cancer-panel sequencing. Excluding 13 samples with low sequence coverage, we further analysed 195 samples. Results Among the 195 women analysed, 39 and 15 were eventually shown to suffer from malignant endometrial and non-endometrial neoplasms, respectively. Driver mutation(s) were found in nine of the 15 cytology-negative patients with endometrial malignancy, corroborating that combination of cytological and genetic analyses should increase the sensitivity of the diagnosis of malignant endometrial neoplasms. Importantly, driver alterations were found in 32 of 125 women without malignant or premalignant diseases, which raises caution for the interpretation of genetic alterations detected in the endometrial samples. Comparison between the driver mutations in the 32 subjects and those in the 29 endometrial malignancies unveiled that the number of mutations and mutations in PTEN, CTNNB1, and TP53 may be applied for the assessment and prediction of development of EC. Conclusions The genetic analysis combined with liquid-based cytology is useful to improve the diagnostic sensitivity of endometrial neoplasms. However, caution must be taken when cancer-associated mutation(s) are detected in the genetic analysis. Further investigation may clarify the risk of malignant endometrial neoplasms in women with driver mutation(s).

  DOI

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Misc 【 display / non-display 】

Misc 【 display / non-display 】

• 子宮体癌と大腸癌におけるミスマッチ修復タンパク欠損と免疫逃避機構の差異に関する検討

  真里谷 奨, 廣橋 良彦, 久保 輝文, 岩崎 雅宏, 鳥越 俊彦, 齋藤 豪

  日本婦人科腫瘍学会学術講演会プログラム・抄録集 ( (公社)日本婦人科腫瘍学会 )  63回   214 - 214  2021.07

• 深層学習を用いた子宮頸部細胞診人工知能診断支援システムの有用性について

  真里谷 奨, 南部 優太, 淺沼 広子, 廣橋 良彦, 岩崎 雅宏, 藤野 雄一, 鳥越 俊彦, 齋藤 豪

  日本癌学会総会記事 ( (一社)日本癌学会 )  79回   OJ15 - 7  2020.10

• マイクロサテライト不安定性およびHLAクラスI分子低発現の子宮内膜癌における臨床的意義(Clinical implications of microsatellite instability and HLA Class I downregulation in endometrial cancer)

  真里谷 奨, 廣橋 良彦, 玉手 雅人, 郷久 晴朗, 久保 輝文, 寺本 瑞絵, 岩崎 雅宏, 鳥越 俊彦, 齋藤 豪

  日本癌学会総会記事 ( (一社)日本癌学会 )  77回   518 - 518  2018.09

• 子宮内膜細胞の悪性化におけるASPP2の役割

  郷久晴朗, 嶋田浩志, 岩崎雅宏, 寺田倫子, 鈴木美和, 秋元太志, 寺本瑞絵, 田中綾一, 阿部秀悦, 齋藤豪

  日本臨床細胞学会雑誌(Web)   56  2017

  J-GLOBAL

• 肥満による子宮内膜癌の悪性化におけるLSR(lipolysis-stimulated lipoprotein receptor)の役割

  嶋田浩志, 郷久晴朗, 寺本瑞絵, 田中綾一, 岩崎雅宏, 小島隆, 齋藤豪

  日本産科婦人科学会雑誌   68 ( 2 )  2016

  J-GLOBAL

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Research Projects 【 display / non-display 】

Research Projects 【 display / non-display 】

• Novel oligomannose liposome-DNA complex DNA vaccination efficiently evokes anti-HPV E6 and E7 CTL responses.

  Grant-in-Aid for Scientific Research (C)

  Project Year :

  2013.04

  -

  2016.03

   

  Saito Tsuyoshi, Iwasaki Masahiro, Tanaka Ryoichi, Sato Noriyuki

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  The aim of this study was to establish an efficient HPV type 16-targeting cancer immunotherapy. HPV16-derived viral proteins E6 and E7 are expressed in cancerous cells through the progression of the disease and have a role in carcinogenesis but are not expressed in normal cells. In this study, we generated a novel HPV 16 E6 and E7 gene plasmid containing oligomannose liposomes (OML-HPV). We compared the cytotoxic T lymphocyte (CTL) induction efficiency of OML-HPV and that of standard liposome-HPV16 E6 and E7 DNA complex. HPV16 E6-specific CTLs could be generated from HPV 16-positive cervical carcinoma patient's peripheral blood mononuclear cells by stimulating OML-HPV, but could not by stimulating standard liposome-HPV 16 E6, E7 DNA complex. These results indicate that OML-HPV is a more effective approach than DNA vaccination using standard liposomes, and that a novel HLA-A24-restricted peptide, E6 66-74, might be a suitable target of cervical cancer immunotherapy.

• Bistandar effect via gap junctional intercellular communication for gene-therapy in gynecologic cancers.

  Grant-in-Aid for Scientific Research (C)

  Project Year :

  2009

  -

  2011

   

  SAITO Tsuyoshi, SUZUKI Takahiro, IWASAKI Masahiro

  　View Summary

  We have confirmed bistandar effect via gap junctional intercellular communication in gynecologic cancers especially endometrial cancer cell. We have also found the bistandar effect was accelerated to add retinoids.