Papers - KURONUMA Kouji

当科における最近の肺非結核性抗酸菌症の臨床的検討

亀田 優美, 山田 玄, 黒沼 幸治, 大塚 満雄, 千葉 弘文, 高橋 弘毅

日本呼吸器学会誌 ( (一社)日本呼吸器学会 )  2 ( 増刊 ) 216 - 216  2013.03

血清SP-D異常低値を呈する肺癌症例の検討

成田 欣史, 黒沼 幸治, 近藤 瞬, 梅田 泰淳, 汐谷 心, 亀田 優美, 北村 康夫, 北田 順也, 大塚 満雄, 千葉 弘文, 山田 玄, 高橋 弘毅

日本呼吸器学会誌 ( (一社)日本呼吸器学会 )  2 ( 増刊 ) 230 - 230  2013.03

Detection of N-glycolyated gangliosides in non-small-cell lung cancer using GMR8 monoclonal antibody.

Nobuyoshi Hayashi, Hirofumi Chiba, Koji Kuronuma, Shinji Go, Yoshihiro Hasegawa, Motoko Takahashi, Shinsei Gasa, Atsushi Watanabe, Tadashi Hasegawa, Yoshio Kuroki, Jinichi Inokuchi, Hiroki Takahashi

Cancer science   104 ( 1 ) 43 - 7  2013.01  [Refereed]  [International journal]

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Gangliosides are glycosphingolipids found on the cell surface. They act as recognition molecules or signal modulators and regulate cell proliferation and differentiation. N-glycolylneuraminic acid (NeuGc)-containing gangliosides have been detected in some neoplasms in humans, although they are usually absent in normal human tissues. Our aim was to evaluate the presence of NeuGc-containing gangliosides including GM3 (NeuGc) and assess their relationship with the prognosis of non-small-cell lung cancer (NSCLC). NeuGc-containing ganglioside expression in NSCLC tissues was analyzed immunohistochemically using the mouse monoclonal antibody GMR8, which is specific for gangliosides with NeuGc alpha 2,3Gal-terminal structures. On the basis of NeuGc-containing ganglioside expression, we performed survival analysis. We also investigated the differences in the effects of GM3 (N-acetylneuraminic acid [NeuAc]) and GM3 (NeuGc) on inhibition of epidermal growth factor receptor (EGFR) tyrosine kinase in A431 cells. As a result, the presence of NeuGc-containing gangliosides was evident in 86 of 93 (93.5%) NSCLC samples. The NSCLC patients with high NeuGc-containing ganglioside expression had a low overall survival rate and a significantly low progression-free survival rate. In the in vitro study, the inhibitory effect of GM3 on EGFR tyrosine kinase in A431 cells after exposure to GM3 (NeuGc) was lower than that after exposure to GM3 (NeuAc). In conclusion, NeuGc-containing gangliosides including GM3 (NeuGc) are widely expressed in NSCLC, and NeuGc-containing ganglioside expression is associated with patient survival. The difference in the effects of GM3 (NeuGc) and GM3 (NeuAc) on the inhibition of EGFR tyrosine kinase might contribute to improvement in the prognosis of NSCLC patients.

DOI PubMed

エンドサイトスコピーを用いた腫瘍性病変の観察

池田 貴美之, 山田 裕一, 梅田 泰淳, 亀田 優美, 成田 欣史, 錦織 博貴, 宮島 さつき, 北田 順也, 黒沼 幸治, 大塚 満雄, 山田 玄, 高橋 弘毅

肺癌 ( (NPO)日本肺癌学会 )  52 ( 5 ) 788 - 788  2012.10

ゲフィチニブ投与後にアルカリフォスファターゼ・フレア現象を呈した肺腺癌の2例

亀田 優美, 梅田 泰淳, 汐谷 心, 成田 欣史, 北村 康夫, 北田 順也, 宮島 さつき, 黒沼 幸治, 大塚 満雄, 山田 玄, 高橋 弘毅

肺癌 ( (NPO)日本肺癌学会 )  52 ( 5 ) 793 - 793  2012.10

Pulmonary surfactant protein A protects lung epithelium from cytotoxicity of human β-defensin 3.

Saito A, Ariki S, Sohma H, Nishitani C, Inoue K, Ebata N, Takahashi M, Hasegawa Y, Kuronuma K, Takahashi H, Kuroki Y

The Journal of biological chemistry ( 18 )  287 ( 18 ) 15034 - 15043  2012.04  [Refereed]

DOI PubMed

A Case of Adult T Cell Leukemia/Lymphoma Presenting as Severe Tracheal Stenosis

Kengo Otsuka, Koji Kuronuma, Mitsuo Otsuka, Junya Kitada, Yoshifumi Narita, Hirotoshi Homma, Satsuki Miyajima, Yasuo Kitamura, Yosuke Suzuki, Motoki Natsuizaka, Hiroshi Yasui, Hiroshi Ikeda, Toshiaki Hayashi, Tadao Ishida, Yasuhisa Shinomura, Hiroko Noguchi, Hiroki Takahashi

INTERNAL MEDICINE ( JAPAN SOC INTERNAL MEDICINE )  50 ( 21 ) 2637 - 2641  2011  [Refereed]

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A 57-year-old woman was referred to our hospital for further examination of a tracheal stenosis shown on computed tomography findings. Bronchoscopy revealed multiple protruding tumors in the lumen of the trachea. Cytological findings of the cell block material from pleural effusion indicated that the T-cell lymphoma was composed of pleomorphic lymphoid cells. Serum human T-cell leukemia virus type1 antibody was positive and supported the clinical diagnosis of ATLL. Systemic chemotherapy induced the remarkable improvement of the lesions, the infiltrative lung shadow and the soft tissue neoplasm. We report a rare case of adult T cell leukemia/lymphoma (ATLL) with endobronchial involvement.

DOI CiNii

ゲフィチニブ奏効後耐性化した肺腺癌に対してエルロチニブへの変更が有効であった当院における経験例

角 俊行, 成田 欣史, 北村 康夫, 北田 順也, 本間 裕敏, 黒沼 幸治, 大塚 満雄, 工藤 和実, 村上 聖司, 山田 玄, 田中 裕士, 高橋 弘毅

肺癌 ( (NPO)日本肺癌学会 )  50 ( 7 ) 946 - 947  2010.12

Pulmonary collectins enhance phagocytosis of Mycobacterium avium through increased activity of mannose receptor.

Kudo K, Sano H, Takahashi H, Kuronuma K, Yokota S, Fujii N, Shimada K, Yano I, Kumazawa Y, Voelker DR, Abe S, Kuroki Y

Journal of Immunology   172 ( 12 ) 7592 - 602  2004  [Refereed]