Organization
Medical Development Center Dean Department of Liberal Arts and Sciences Biology Associate Professor
Title
Associate Professor
External link
SUZUKI Takeshi
Research Interests
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免疫シナプス
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超分子複合体
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ライブセルイメージング
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膜タンパク質
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糖輸送体
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免疫シナプス
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超分子複合体
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ライブセルイメージング
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シグナル伝達
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シグナル伝達
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分子動態
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細胞膜
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細胞極性
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分子動態
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細胞膜
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細胞極性
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膜タンパク質
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糖輸送体
Research Areas
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Life Science / Anatomy
Education
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The University of Tokyo Graduate School, Division of Science
- 1994
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The University of Tokyo
- 1994
Country： Japan
Professional Memberships
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日本顕微鏡学会
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日本細胞生物学会
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The American Society for Cell Biology
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日本組織細胞化学会
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日本解剖学会
Committee Memberships
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日本組織細胞化学会 評議員
Papers
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Adaptive restoration of T-cell motility by cold acclimation through metabolic and transcriptional remodeling. International journal
Masahiro Hattori, Noriyoshi Onishi, Tatsuya Sato, Takashi Nakakura, Takeshi Suzuki
Frontiers in immunology 17 1789182 - 1789182 2026
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Regulation of Aqp1 expression by osmotic balance in fenestrated endothelial cells of the posterior lobe of the pituitary. International journal
Takashi Nakakura, Takeshi Suzuki
Cell and tissue research 401 ( 3 ) 265 - 274 2025.9
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Collagen XIII is the Key Molecule of Neurovascular Junctions in the Neuroendocrine System. Reviewed International journal
Takashi Nakakura, Kotaro Horiguchi, Takeshi Suzuki
Neuroendocrinology 114 ( 7 ) 658 - 669 2024.4
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Caveolae-mediated endocytosis pathway regulates endothelial fenestra homeostasis in the rat pituitary. International journal
Takashi Nakakura, Hideyuki Tanaka, Takeshi Suzuki
Biochemical and biophysical research communications 675 177 - 183 2023.7
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Regulation of fenestra formation via actin-dynamin2 interaction in rat pituitary endothelial cells
Takashi Nakakura, Hideyuki Tanaka, Takeshi Suzuki
Cell and Tissue Research 2022.9
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Fibronectin-integrin signaling regulates PLVAP localization at endothelial fenestrae by microtubule stabilization. International journal
Takashi Nakakura, Takeshi Suzuki, Kotaro Horiguchi, Hideyuki Tanaka, Kenjiro Arisawa, Toshio Miyashita, Yoko Nekooki-Machida, Haruo Hagiwara
Cell and tissue research 384 ( 2 ) 449 - 463 2021.5
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Fibronectin is essential for formation of fenestrae in endothelial cells of the fenestrated capillary. International journal
Takashi Nakakura, Takeshi Suzuki, Hideyuki Tanaka, Kenjiro Arisawa, Toshio Miyashita, Yoko Nekooki-Machida, Toshiki Kurosawa, Yuma Tega, Yoshiharu Deguchi, Haruo Hagiwara
Cell and tissue research 383 ( 2 ) 823 - 833 2021.2
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ATAT1 is essential for regulation of homeostasis-retaining cellular responses in corticotrophs along hypothalamic-pituitary-adrenal axis Reviewed
Takashi Nakakura, Takeshi Suzuki, Seiji Torii, Anshin Asano-Hoshino, Yoko Nekooki-Machida, Hideyuki Tanaka, Kenjiro Arisawa, Yoshimi Nishijima, Takao Susa, Tomoki Okazaki, Yoshiko Kiuchi, Haruo Hagiwara
CELL AND TISSUE RESEARCH 370 ( 1 ) 169 - 178 2017.10
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Expression and localization of forkhead box protein FOXJ1 in S100β-positive multiciliated cells of the rat pituitary. Reviewed
Takashi Nakakura, Takeshi Suzuki, Kotaro Horiguchi, Ken Fujiwara, Takehiro Tsukada, Anshin Asano-Hoshino, Hideyuki Tanaka, Kenjiro Arisawa, Yoshimi Nishijima, Yoko Nekooki-Machida, Yoshiko Kiuchi, Haruo Hagiwara
Medical molecular morphology 50 ( 2 ) 59 - 67 2017.6
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Adrenalectomy facilitates ATAT1 expression and alpha-tubulin acetylation in ACTH-producing corticotrophs Reviewed
Takashi Nakakura, Takahiro Nemoto, Takeshi Suzuki, Anshin Asano-Hoshino, Hideyuki Tanaka, Kenjiro Arisawa, Yoshimi Nishijima, Yoshiko Kiuchi, Haruo Hagiwara
CELL AND TISSUE RESEARCH 366 ( 2 ) 363 - 370 2016.11
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Intracellular localization of alpha-tubulin acetyltransferase ATAT1 in rat ciliated cells Reviewed
Takashi Nakakura, Takeshi Suzuki, Takahiro Nemoto, Hideyuki Tanaka, Anshin Asano-Hoshino, Kenjiro Arisawa, Yoshimi Nishijima, Yoshiko Kiuchi, Haruo Hagiwara
MEDICAL MOLECULAR MORPHOLOGY 49 ( 3 ) 133 - 143 2016.9
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The elongation of primary cilia via the acetylation of alpha-tubulin by the treatment with lithium chloride in human fibroblast KD cells Reviewed
Takashi Nakakura, Anshin Asano-Hoshino, Takeshi Suzuki, Kenjiro Arisawa, Hideyuki Tanaka, Yoshihisa Sekino, Yoshiko Kiuchi, Kazuhiro Kawai, Haruo Hagiwara
MEDICAL MOLECULAR MORPHOLOGY 48 ( 1 ) 44 - 53 2015.3
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Visual Basic macro-program for Microsoft Word useful for planning the recombinant DNA experiments Reviewed
T Suzuki, K Tanaka
J Cent Med Edu Sapporo Med Univ 5 ( 5 ) 5 - 10 2014
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Bio-Imaging of Alpha-Tubulin Acetyltransferase in Living Mammalian Cells Reviewed
T Suzuki, K Nakakura, H Hagiwara
J Cent Med Edu Sapporo Med Univ 4 ( 4 ) 11 - 15 2013
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Function of the Membrane Water Channel Aquaporin-5 in the Salivary Gland Reviewed
Toshiyuki Matsuzaki, Taketo Susa, Kinue Shimizu, Nobuhiko Sawai, Takeshi Suzuki, Takeo Aoki, Satoshi Yokoo, Kuniaki Takata
ACTA HISTOCHEMICA ET CYTOCHEMICA 45 ( 5 ) 251 - 259 2012
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Close Association of Aquaporin-2 Internalization with Caveolin-1 Reviewed
Takeo Aoki, Takeshi Suzuki, Haruo Hagiwara, Michio Kuwahara, Sei Sasaki, Kuniaki Takata, Toshiyuki Matsuzaki
ACTA HISTOCHEMICA ET CYTOCHEMICA 45 ( 2 ) 139 - 146 2012
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Nonlinear Phase Imaging Using Two-Beam Interferometry in Stimulated Parametric Emission Microscopy Reviewed
Masatomo Yamagiwa, Yasuyuki Ozeki, Gen Omura, Takeshi Suzuki, Shin'ichiro Kajiyama, Kiichi Fukui, Kazuyoshi Itoh
JAPANESE JOURNAL OF APPLIED PHYSICS 48 ( 6 ) 062501 2009.6
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Immunohistochemical and electron microscopic observations of stromal cells in the human oviduct mucosa Reviewed
Haruo Hagiwara, Nobuo Ohwada, Takeo Aoki, Takeshi Suzuki, Kuniaki Takata
MEDICAL MOLECULAR MORPHOLOGY 41 ( 4 ) 221 - 226 2008.12
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The primary cilia of secretory cells in the human oviduct mucosa Reviewed
Haruo Hagiwara, Nobuo Ohwada, Takeo Aoki, Takeshi Suzuki, Kuniaki Takata
MEDICAL MOLECULAR MORPHOLOGY 41 ( 4 ) 193 - 198 2008.12
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Immunolocalization of water channel aquaporins in the vomeronasal organ of the rat: Expression of AQP4 in neuronal sensory cells. Reviewed
Abduxukur Ablimit, Takeo Aoki, Toshiyuki Matsuzaki, Takeshi Suzuki, Haruo Hagiwara, Shigeru Takami, Kuniaki Takata
Chem Senses 33 ( 5 ) 481?488 - 488 2008
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Differential localization of aquaporin-2 and glucose transporter 4 in polarized MDCK cells Reviewed
Takahiro Hasegawa, Toshiyuki Matsuzaki, Yuki Tajika, Abduxukur Ablimit, Takeshi Suzuki, Takeo Aoki, Haruo Hagiwara, Kuniaki Takata
HISTOCHEMISTRY AND CELL BIOLOGY 127 ( 3 ) 233 - 241 2007.3
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Recent advances in fluorescent labeling techniques for fluorescence microscopy Reviewed
Takeshi Suzuki, Toshiyuki Matsuzaki, Haruo Hagiwara, Takeo Aoki, Kuniaki Takata
ACTA HISTOCHEMICA ET CYTOCHEMICA 40 ( 5 ) 131 - 137 2007
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Localization of Golgi 58K protein (formiminotransferase cyclodeaminase) to the centrosome Reviewed
Haruo Hagiwara, Yuki Tajika, Toshiyuki Matsuzaki, Takeshi Suzuki, Takeo Aoki, Kuniaki Takata
HISTOCHEMISTRY AND CELL BIOLOGY 126 ( 2 ) 251 - 259 2006.8
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Expression and immunolocalization of water-channel aquaporins in the rat and mouse mammary gland Reviewed
T Matsuzaki, N Machida, Y Tajika, A Ablimit, T Suzuki, T Aoki, H Hagiwara, K Takata
HISTOCHEMISTRY AND CELL BIOLOGY 123 ( 4-5 ) 501 - 512 2005.6
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Aquaporin water channels in the kidney Reviewed
K Takata, T Matsuzaki, Y Tajika, A Ablimit, T Suzuki, T Aoki, H Hagiwara
ACTA HISTOCHEMICA ET CYTOCHEMICA 38 ( 3 ) 199 - 207 2005
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Water channel aquaporin 1 (AQP1) is present in the perineurium and perichondrium Reviewed
T Matsuzaki, A Ablimit, Y Tajika, T Suzuki, T Aoki, H Hagiwara, K Takata
ACTA HISTOCHEMICA ET CYTOCHEMICA 38 ( 1 ) 37 - 42 2005
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Immunohistochemical characterization of the intracellular pool of water channel aquaporin-2 in the rat kidney.
Yuki Tajika, Toshiyuki Matsuzaki, Takeshi Suzuki, Takeo Aoki, Haruo Hagiwara, Shigeyasu Tanaka, Eiki Kominami, Kuniaki Takata
Anatomical science international 77 ( 3 ) 189 - 95 2002.9
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Aquaporins: a water channel family. Reviewed
Matsuzaki T, Tajika Y, Tserentsoodol N, Suzuki T, Aoki T, Hagiwara H, Takata K
Anatomical science international 77 ( 2 ) 85 - 93 2002.6
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Polarized distribution of Na+-dependent glucose cotransporter SGLT1 in epithelial cells Reviewed
T Suzuki, T Matsuzaki, K Takata
ACTA HISTOCHEMICA ET CYTOCHEMICA 33 ( 3 ) 159 - 162 2000
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Plastid Gene Expression during Amyloplast Formation in Cultured Tobacco Cells Reviewed
Atsushi Sakai, Yutaka Miyazawa, Takeshi Suzuki, Narie Sasaki, Shigeyuki Kawano, Tsuneyoshi Kuroiwa
Journal of Plant Physiology 154 ( 1 ) 71 - 78 1999.1
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Comparative analysis of DNA synthesis activity in plastid-nuclei and mitochondrial-nuclei simultaneously isolated from cultured tobacco cells
A Sakai, T Suzuki, N Nagata, N Sasaki, Y Miyazawa, C Saito, N Inada, Y Nishimura, T Kuroiwa
PLANT SCIENCE 140 ( 1 ) 9 - 19 1999.1
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Simultaneous isolation of cell-nuclei, plastid-nuclei and mitochondrial- nuclei from cultured tobacco cells; comparative analysis of their transcriptional activities in vitro Reviewed
Atsushi Sakai, Takeshi Suzuki, Yutaka Miyazawa, Tsuneyoshi Kuroiwa
Plant Science 133 ( 1 ) 17 - 31 1998.4
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P-1 Nuclear staining fluorescent dyes suitable for laser confocal microscopy :
MATSUZAKI Toshiyuki, SUZUKI Takeshi, TAKATA Kuniaki, HASHIGUCHI Shin, HIRATA Masazumi, MURATA Hiroaki, TAKESHITA Hideyuki, KUSUZAKI Katsuyuki, KONISHI Eiichi, HIRASAWA Yasusuke, ASHIHARA Tsukasa, Suginoshita T., Kusuzaki K., Hashiguchi S., Hirata M., Fukuroku J., Urata Y., Hirasawa Y., Ashihara T., KAWAI Kanji, UEDA Kazushige, OCHIAI Toshiya, OGINO Atsuhiro, ITOI Hirosumi, YAMAGISHI Hisakazu, URATA Yoji, OKA Takahiro, ASHIHARA Tsukasa, YAMAASHI Toshiaki, AKITA Masahiko, TANOOKA Ken, SETSU Kojun, Maezawa Yasuhisa, Baba Hisatoshi, Furusawa Nobuaki, Uchida Kenzou, Imura Shinichi, TAMADA Yoshitaka, HAYASHI Seiji, IIJIMA Norio, IKE Hiromi, ISHIHARA Akihiko, TANAKA Masaki, SUWA Fumihiko, IBATA Yasuhiko, Kimura Masaru, SUGA Hiroyasu, MIYOSI Norio, NAKAGAWA Takao, FUKUDA Masaru, Zinchuk Vadim S., Okada Teruhiko, Kobayashi Toshihiro, Saz Eva Garcia del, Seguchi Harumichi, Zhang Youyun, Dai Jibin, Zhou Xinhua, Dong Fong, HEMMI Akihiro, KOMIYAMA Akira, OHNO Shinichi, KATOH Ryohei, Takeshita Hideyuki, Kusuzaki Katsuyuki, Tsuji Yoshiro, Hirata Masazumi, Hashiguchi Shin, Hirasawa Yasusuke, Ashihara Tsukasa, MORIKAWA Shigeru, TORII Ikuko, NAGASAKI Makoto, MISHIMA Satoko, Mizoguchi Akira, Ide Chizuka, NAITO Ichiro, INOUE Satoko, SENO Satimaru, WATANABE Jun, KONDO Kazumasa, Mino Kazuto, KANAMURA Shinsuke, CHIDA Kohsuke, GOTO Tetusya, TANAKA Teruo, TAKAMI Shigeru, ODA Tatsuroh, NISHIYAMA Fumiaki, Wakayama Tomohiko, Iseki Shoichi, KHALED AHMED, NORIKI S., MAEGAWA H., FUKUDA M., Jiang Mingsen, Yu Zujiang, Yang Mingyi, Dong Huifen, UENO Masaki, FUTAESAKU Yutaka, KOZUKA Yoshimichi, YANO Misai, ONO Michiko, FUTAESAKU Yutaka, SUMI Yawara, Itoh Masanori T., YOSHIDA Minoru, Ito Atsuko, Hayashi Michiko, Hoshida Minako, Ito Kinji, NAKAZATO Kyoumi, SUZUKI Keiji, NAKAJIMA Katuyuki, SAGA Tsuyoshi, YOSHIZUKA Mitsuaki, Nemoto Norimichi, Lu Wei, Nakamura Hitomi, Hayakawa Satoshi, Chishima Fuminao, WATANABA Akio, KAWAOI Akira, KRIA Lidia, OHIRA Akihiro, AMEMIYA Tsugio, KARAYA Kazuhiro, KONDO Takashi, UMEMURA Shinobu, YASUDA Masanori, ITOH Johbu, TAKEKOSHI Susumu, OSAMURA Yoshiyuki, WATANABE Keiichi, SASAKI Yukihiro, AHMED Helen, TAKEUCHI Takumi, UEKI Tetsuo, KAJIWARA Takahiro, MORIYAMA Nobuo, KAWABE Kazuki, YOKOI Hiromichi, YAMAMA Yoshihiro, TSURUO Yoshihiro, ISHIMURA Kazunori, Kato Yoichiro, Yamamoto Tomoko, Kobayashi Makio, KOMIYAMA Shin-ichi, AOKI Daisuke, TOMINAGA Eiichiro, SUSUMU Nobuyuki, UDAGAWA Yasuhiro, NOZAWA Shiro, MURATA Hideaki, URATA Youzi, Ito Toshihisa, HORITA Kohjiro, IMAMURA Yoshiaki, NORIKI Sakon, NAKAGAWARA Gizo, Mo Yiqun, Zhang Qunwei, Yamaguchi Akio, Horita Kohjiro, Zheng Shu, Leng Chong-Guang, Ueda Hideho, Fujii Yasuhisa, Terada Nobuo, Baba Takeshi, Yamazaki S., Kameyama S., Fukasawa R., Moriyama N., Kawabe K., KOBAYASHI Yasuhiro, KAWAKAMI Hayato, YOSHINO Yoshikazu, HIRANO Hiroshi, AKIMOTO Yoshihiro, KREPPEL Lisa K., HART Gerald W., KAWASHIMA Kenji, NAKAZATO Kyomi, HIRAISHI Katsuya, UEHARA Katsuaki, SHIMADA Junichi, FUSHIKI Shinji, Susumu Nobuyuki, ARAI Ryohachi, SAKAI Kazuyoshi, NAGATSU Ikuko, Shin Bo-Chul, ASAKAWA Yoshihiro, KOMURO Masato, Zhou Lanxian, Yuan Hua, Hu Jialuo, Huang Wenduo, Wang Xiaoyun, MIYAMOTO Yohei, SHIMBO Mari, TAHARA Shigeyuki, SUGIYAMA Makoto, TAKUMI Ichiro, SANNO Naoko, TERAMOTO Akira, MATSUDA Minoru, FUKUSHIMA Hisaki, TANAKA Ryota, SANTO Ikuya, HANAOKA Tateo, GOYA Tomoyuki, KUDO Akihiko, HIRANO Hiroshi, Kudo Akihiko
Acta histochemica et cytochemica 31 ( 2 ) 152 - 152 1998
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Colocalization of tight junction proteins, occludin and ZO-1, and glucose transporter GLUT1 in cells of the blood-ocular barrier in the mouse eye Reviewed
Nomingerel Tserentsoodol, Bo-Chul Shin, Takeshi Suzuki, Kuniaki Takata
Histochemistry and Cell Biology 110 ( 6 ) 543 - 551 1998
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1,8-cineole inhibits root growth and DNA synthesis in the root apical meristem of Brassica campestris L Reviewed
R Koitabashi, T Suzuki, T Kawazu, A Sakai, H Kuroiwa, T Kuroiwa
JOURNAL OF PLANT RESEARCH 110 ( 1097 ) 1 - 6 1997.3
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Amyloplast formation in cultured-tobacco cells II: Effects of transcription/translation inhibitors on accumulation of starch Reviewed
Atsushi Sakai, Yutaka Miyazawa, Kumiko Yashiro, Takeshi Suzuki, Shigeyuki Kawano
Cytologia 62 ( 3 ) 295 - 301 1997
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Connexin 43 and the glucose transporter, GLUT1, in the ciliary body of the rat Reviewed
Bo-Chul Shin, Takeshi Suzuki, Shigeyasu Tanaka, Akio Kuraoka, Yosaburo Shibata, Kuniaki Takata
Histochemistry and Cell Biology 106 ( 2 ) 209 - 214 1996
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Organelle DNA synthesis in the quiescent centre of Arabidopsis thaliana (col.)
Makoto Fujie, Haruko Kuroiwa, Takeshi Suzuki, Shigeyuki Kawano, Tsuneyoshi Kuroiwa
Journal of Experimental Botany 44 ( 4 ) 689 - 693 1993.4
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NUCLEOTIDE-SEQUENCE OF THE F1-ATPASE ALPHA-SUBUNIT GENE FROM RICE MITOCHONDRIA
K KADOWAKI, S KAZAMA, T SUZUKI
NUCLEIC ACIDS RESEARCH 18 ( 5 ) 1302 - 1302 1990.3
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NUCLEOTIDE-SEQUENCE OF THE CYTOCHROME-OXIDASE SUBUNIT-I GENE FROM RICE MITOCHONDRIA Reviewed
K KADOWAKI, T SUZUKI, S KAZAMA, T OHFUCHI, W SAKAMOTO
NUCLEIC ACIDS RESEARCH 17 ( 18 ) 7519 - 7519 1989.9
Books
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バイオイメージングで知っておきたい顕微鏡の基礎
鈴木 健史（pp. 143-158）
日本組織細胞化学会編、組織細胞化学2012 2012
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共焦点レーザー顕微鏡および蛍光顕微鏡を使いこなすための基礎知識
鈴木 健史（ Role： Contributorpp. 103-115）
日本組織細胞化学会編、組織細胞化学2011 2011.8
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Double-label immunoelectron microscopy for studying the colocalization of proteins in cultured cells.
Immunoelectron microscopy, Methods in Molecular Biology 657, Schwartzbach and Osafune (eds), Springer Science+Business Media, New York 2010
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Pre-embedding immunoelectron microscopy of chemically fixed mammalian tissue culture cells.
H Hagiwara, T Aoki, T Suzuki, K Takata（ Role： Joint authorSchwartzbach SD, Osafune T, eds, Immunoelectron microscopy, methods and protocols）
New York: Human Press 2010
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Double-label immunoelectron microscopy for studying the colocalization of proteins in culture cells.
H Hagiwara, T Aoki, T Suzuki, K Takata（ Role： Joint authorSchwartzbach SD, Osafune T, eds, Immunoelectron microscopy, methods and protocols）
New York: Human Press 2010
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Double-label immunoelectron microscopy for studying the colocalization of proteins in cultured cells.
Immunoelectron microscopy, Methods in Molecular Biology 657, Schwartzbach and Osafune (eds), Springer Science+Business Media, New York 2010
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蛍光タンパク質の基礎と実際
中西印刷 2008
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ボケを除く処理：デコンボリューション顕微鏡法。
染色・バイオイメージング実験ハンドブック。細胞や組織の形態・遺伝子・タンパク質を観るための染色法と顕微鏡観察のすべて 羊土社 2006
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バイオイメージングがわかる（細胞内分子を観察する多様な技術とその原理）画像処理の原理と効果
羊土社 2005
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免疫組織化学：どの方法を選ぶか。
組織細胞化学2003(日本組織細胞化学会編），学際企画，東京 2003
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デコンボリューション顕微鏡法
初めてでもできる共焦点顕微鏡活用プロトコール 実験医学別冊、羊土社、東京 2003
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蛍光抗体法-基礎から多重染色まで。
組織細胞化学2002、学際企画 2002
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免疫電顕法の実際－どんな時にどの方法を使うか－．
組織細胞化学２００２ 2002
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免疫組織化学の基礎的手順--抗体入手から検鏡まで．組織細胞化学
2001（日本組織細胞化学会編），学際企画，東京 2001
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Water channel protein, aquaporin 3, in epithelial cells. In: Molecular Biology and Physiology of Water and Solute Transport
Hohmann & Neilsen (eds), Kluwer Academic/Plenum Publishers, New York 2000
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Polarized distribution of Na+-dependent glucose cotransporter SGLT1 in epithelial cells
Acta Histochem Cytochem 2000
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Water channel protein, aquaporin 3, in epithelial cells.
Hohmann, Neilsen eds., Molecular Biology and Physiology of Water and Solute Transport, Kluwer Academic/Plenum Publishers, New York 2000
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Water channel protein, aquaporin 3, in epithelial cells. In: Molecular Biology and Physiology of Water and Solute Transport
Hohmann & Neilsen (eds), Kluwer Academic/Plenum Publishers, New York 2000
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未知の抗体を使っての染色の進め方
組織細胞化学2000（日本組織細胞化学会編），学際企画，東京 2000
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ボケを除く処理 - デコンボリューション顕微鏡法．
高田邦昭、川上速人、斎藤尚亮編，染色・バイオイメージング実験ハンドブック，羊土社，東京 2000 （ ISBN:4758108048 ）
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Polarized distribution of Na+-dependent glucose cotransporter SGLT1 in epithelial cells
Acta Histochem Cytochem 2000
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未知の抗体を使っての染色の進め方．
日本組織細胞化学会編，組織細胞化学 2000，学際企画，東京 2000 （ ISBN:4906514375 ）
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Water channel protein, aquaporin 3, in epithelial cells.
Hohmann, Neilsen eds., Molecular Biology and Physiology of Water and Solute Transport, Kluwer Academic/Plenum Publishers, New York 2000
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細胞への遺伝子導入と組織化学．
日本組織細胞化学会編，組織細胞化学 1999，学際企画，東京 1999 （ ISBN:4906514340 ）
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共焦点顕微鏡を使ったNa+依存型糖輸送体SGLT1局在機構の解析
電子顕微鏡 1999
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レーザー共焦点顕微鏡法における蛍光抗体染色と核酸対比染色
日本比較内分泌学会ニュース 1997
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蛍光抗体法．
日本組織細胞化学会編，組織細胞化学 1996，学際企画，東京 1996 （ ISBN:4906514227 ）
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ギャップ結合と糖輸送
Mebio 1995
MISC
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線維芽細胞における一次線毛伸長について
萩原治夫, 中倉敬, 浅野安信, 有澤謙二郎, 田中秀幸, 鈴木健史
日本臨床分子形態学会総会・学術集会講演プログラム・要旨集 45th 98 2013
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共焦点レーザー顕微鏡および蛍光顕微鏡を使いこなすための基礎知識(C.共焦点顕微鏡と画像技術,見るバイオサイエンス-基礎から最先端技術まで)
鈴木 健史
組織細胞化学 2011 103 - 115 2011.7
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頂部膜剥離法によって作製した試料によるAQP2動態の観察
青木武生, 萩原治夫, 鈴木健史, 松崎利行, 高田邦昭
解剖学雑誌 85 ( Supplement ) 2010
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Pre-embedding immunoelectron microscopy of chemically fixed mammalian tissue culture cells
Hagiwara H, Aoki T, Suzuki T, Takata K
Methods in Molecular Biology 657 145 - 54 2010
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Double-label immunoelectron microscopy for studying the colocalization of proteins in cultured cells
Hagiwara H, Aoki T, Suzuki T, Takata K
Methods in Molecular Biology 657 249 - 57 2010
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Roles of gap junctions in glucose transport from glucose transporter 1-positive to -negative cells in the lateral wall of the rat cochlea
Toshihiro Suzuki, Tatsuya Matsunami, Yasuo Hisa, Kuniaki Takata, Tetsuro Takamatsu, Masahito Oyamada
HISTOCHEMISTRY AND CELL BIOLOGY 131 ( 1 ) 89 - 102 2009.1
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PI-28 MDCK細胞における頂部細胞膜カベオラと水チャネルアクアポリン2との関係(免疫組織化学,ポスター1,第50回日本組織細胞化学会総会・学術集会)
青木 武生, 萩原 治夫, 鈴木 健史, 高田 邦昭
日本組織細胞化学会総会プログラムおよび抄録集 ( 50 ) 76 - 76 2009
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蛍光タンパク質の基礎と実際(E.顕微鏡観察技術の新しい展開)
鈴木 健史, 高田 邦昭
組織細胞化学 2008 101 - 109 2008.7
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Internalization of caveolae and their relationship with endosomes in cultured human and mouse endothelial cells
Takeo Aoki, Haruo Hagiwara, Toshiyuki Matsuzaki, Takeshi Suzuki, Kuniaki Takata
ANATOMICAL SCIENCE INTERNATIONAL 82 ( 2 ) 82 - 97 2007.6
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水チャネル・アクアポリン2のエンドサイトーシスはクラスリン関連経路と関係あるのか?
青木武生, 鈴木健史, 萩原治夫, 長谷川敬展, 高田邦昭
解剖学雑誌 82 ( Supplement ) 135 2007.3
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鈴木 健史, 高田 邦昭, Kupfer Abraham
日本組織細胞化学会総会プログラムおよび抄録集 ( 48 ) 52 - 52 2007
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Changes of aquaporin 5-distribution during release and reaccumulation of secretory granules in isoproterenol-treated mouse parotid gland
Toshiyuki Matsuzaki, Abduxukur Ablimit, Takeshi Suzuki, Takeo Aoki, Haruo Hagiwara, Kuniaki Takata
JOURNAL OF ELECTRON MICROSCOPY 55 ( 3 ) 183 - 189 2006.6
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Gap junctions mediate glucose transport between GLUT1-positive and -negative cells in the spiral limbus of the rat cochlea
T Matsunami, T Suzuki, Y Hisa, K Takata, T Takamatsu, M Oyamada
CELL COMMUNICATION AND ADHESION 13 ( 1-2 ) 93 - 102 2006.1
-
Deconvolution microscopy Reviewed
SUZUKI Takeshi, TAKATA Kuniaki
Denshi kenbikyo 41 ( 2 ) 120 - 123 2006
-
Apical localization of sodium-dependent glucose transporter SGLT1 is maintained by cholesterol and microtubules
Takeshi Suzuki, Toshiyuki Matsuzaki, Haruo Hagiwara, Takeo Aoki, Yukiko Tajika-Takahashi, Kuniaki Takata
ACTA HISTOCHEMICA ET CYTOCHEMICA 39 ( 6 ) 155 - 161 2006
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アクアポリン2水チャネルの細胞内貯蔵部位と細胞内動態
高田邦昭, 多鹿友喜, 松崎利行, 鈴木健史, 青木武生, 萩原治夫
解剖学雑誌 81 ( Supplement ) 2006
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Differential regulation of AQP2 trafficking in endosomes by microtubules and actin filaments
Y Tajika, T Matsuzaki, T Suzuki, A Ablimit, T Aoki, H Hagiwara, M Kuwahara, S Sasaki, K Takata
HISTOCHEMISTRY AND CELL BIOLOGY 124 ( 1 ) 1 - 12 2005.7
-
IB-25 Aquaporins in the rat nasal mucosa :
Ablimit Abduxukur, Matsuzaki Toshiyuki, Suzuki Takeshi, Aoki Takeo, Hagiwara Haruo, Takata Kuniaki
( 46 ) 77 - 77 2005
-
授乳期ラット乳腺におけるアクアポリン水チャネルの分布局在
松崎利行, 多鹿友喜, ABLIMIT Abdushukur, 鈴木健史, 青木武生, 萩原治夫, 高田邦昭
解剖学雑誌 80 ( 1 ) 2005
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共焦点顕微鏡活用法．
鈴木 健史, 松崎 利行, 高田 邦昭
Surgery Frontier 12(4),pp 82-85 2005
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Matsuzaki Toshiyuki, Tajika Yuki, Ablimit Abdushukur, Suzuki Takeshi, Aoki Takeo, Hagiwara Haruo, Takata Kuniaki
Acta histochemica et cytochemica 38 ( 2 ) 173 - 173 2005
-
Molecular mechanisms and drug development in aquaporin water channel diseases: Water channel aquaporin-2 of kidney collecting duct cells
Kuniaki Takata, Yuki Tajika, Toshiyuki Matsuzaki, Takeo Aoki, Takeshi Suzuki, Ablimit Abduxukur, Haruo Hagiwara
Journal of Pharmacological Sciences 96 ( 3 ) 255 - 259 2004.11
-
Aquaporin-2 is retrieved to the apical storage compartment via early endosomes and phosphatidylinositol 3-kinase-dependent pathway
Y Tajika, T Matsuzaki, T Suzuki, T Aoki, H Hagiwara, M Kuwahara, S Sasaki, K Takata
ENDOCRINOLOGY 145 ( 9 ) 4375 - 4383 2004.9
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Tajika Yuki, Matsuzaki Toshiyuki, Suzuki Takeshi, Aoki Takeo, Hagiwara Haruo, Takata Kuniaki
Acta histochemica et cytochemica 37 ( 1 ) 48 - 48 2004
-
ラット乳腺における水チャネルAQP1およびAQP3の局在
松崎利行, 多鹿友喜, ABLIMIT A, 鈴木健史, 青木武生, 萩原治夫, 高田邦昭
日本組織細胞化学会総会・学術集会講演プログラム・予稿集 45th 2004
-
Immunolocalization of the water channel, aquaporin-5 (AQP5), in the rat digestive system
T Matsuzaki, Y Tajika, T Suzuki, T Aoki, H Hagiwara, K Takata
ARCHIVES OF HISTOLOGY AND CYTOLOGY 66 ( 4 ) 307 - 315 2003.10
-
免疫組織化学 : どの方法を選ぶか(II.免疫組織化学 : 基礎から応用まで)
高田 邦昭, 多鹿 友喜, 松崎 利行, 鈴木 健史, 青木 武生, 萩原 治夫
組織細胞化学 2003 17 - 24 2003.7
-
A simple electroporation method for the introduction of plasmids into cells cultured on coverslips for histochemical examination.
TAKATA KUNIAKI, TAJIKA TAKAHASHI YUKIKO, MATSUZAKI TOSHIYUKI, TAJIKA YUKI, SUZUKI TAKESHI, AOKI TAKEO, HAGIWARA HARUO
Acta Histochem Cytochem 36 ( 4 ) 317 - 323 2003
-
Cryosectioning of cultured cells on permeable support
Y Tajika, T Matsuzaki, T Suzuki, T Aoki, H Hagiwara, K Takata
ACTA HISTOCHEMICA ET CYTOCHEMICA 36 ( 2 ) 119 - 122 2003
-
カベオラの内在化と膜動態
青木武生, 萩原治夫, 松崎利行, 鈴木健史, 多鹿友喜, 高田邦昭
解剖学雑誌 78 ( Supplement ) 2003
-
Introduction and expression of glucose transporters in pancreatic acinar cells by in vivo electroporation
Y Shinoda, T Matsuzaki, M Yokoo-Sugawara, T Suzuki, T Aoki, H Hagiwara, H Kuwano, K Takata
ACTA HISTOCHEMICA ET CYTOCHEMICA 36 ( 1 ) 77 - 82 2003
-
Immunolocalization of water channel, aquaporin-5 (AQP5) in the rat digestive system.
MATSUZAKI TOSHIYUKI, TAJIKA YUKI, SUZUKI TAKESHI, AOKI TAKEO, HAGIWARA HARUO, TAKATA KUNIAKI
Arch Histol Cytol 66 ( 4 ) 307 - 315 2003
-
A simple electroporation method for the introduction of plasmids into cells cultured on coverslips for histochemical examination
K Takata, Y Takahashi, T Matsuzaki, Y Tajika, T Suzuki, T Aoki, H Hagiwara
ACTA HISTOCHEMICA ET CYTOCHEMICA 36 ( 4 ) 317 - 323 2003
-
免疫電顕法の実際 : どんな時にどの方法を使うか
萩原 治夫, 青木 武生, 鈴木 健史, 松崎 利行, 高田 邦昭
組織細胞化学 2002 53 - 64 2002.7
-
蛍光抗体法 : 基礎から多重染色まで
青木 武生, 松崎 利行, 鈴木 健史, 萩原 治夫, 多鹿 友喜, 高田 邦昭
組織細胞化学 2002 33 - 41 2002.7
-
Aquaporins: a water channel family.
MATSUZAKI TOSHIYUKI, TAJIKA YUKI, Nomingerel Tserentsoodol, SUZUKI TAKESHI, AOKI TAKEO, HAGIWARA HARUO, TAKATA KUNIAKI
Anat Sci Int 77 ( 2 ) 85 - 93 2002
-
Immunohistochemical characterization of the intracellular pool of water channel aquaporin-2 in the rat kidney.
TAJIKA YUKI, MATSUZAKI TOSHIYUKI, SUZUKI TAKESHI, AOKI TAKEO, HAGIWARA HARUO, Shigeyasu Tanaka, Eiki Kominami, TAKATA KUNIAKI
Anat Sci Int 77 ( 3 ) 189 2002
-
Activation of organelle DNA synthesis during the initial phase of proliferation of BY-2 cultured tobacco cells after medium renewal
Saori Okamura, Takeshi Suzuki, Yutaka Miyazawa, Tsuneyoshi Kuroiwa, Atsushi Sakai
PLANT MORPHOLOGY 14,pp 16-28 ( 1 ) 16 - 28 2002
-
多細胞化へのみち,細胞接着の細胞生物学
鈴木 健史, 高田 邦昭
黒岩常祥，中野明彦，財団法人遺伝学普及会偏，細胞のミクロコスモス－進化とゲノムからその素顔にせまる－，裳華房，東京 pp 157-165 2002
-
A close relationship between EEA1 and internalized caveolae structure :
Aoki Takeo, Hagiwara Haruo, Suzuki Takeshi, Matsuzaki Toshiyuki, Takata Kuniaiki
Acta histochemica et cytochemica 35 ( 1 ) 59 - 59 2002
-
多細胞化へのみち--細胞接着の細胞生物学 (細胞のミクロコスモス--進化とゲノムからその素顔にせまる)
鈴木 健史, 高田 邦昭
生物の科学「遺伝」別冊 ( 14 ) 157 - 165 2002
-
Aquaporins: a water channel family.
MATSUZAKI TOSHIYUKI, TAJIKA YUKI, Nomingerel Tserentsoodol, SUZUKI TAKESHI, AOKI TAKEO, HAGIWARA HARUO, TAKATA KUNIAKI
Anat Sci Int 77 ( 2 ) 85 - 93 2002
-
Immunohistochemical characterization of the intracellular pool of water channel aquaporin-2 in the rat kidney.
TAJIKA YUKI, MATSUZAKI TOSHIYUKI, SUZUKI TAKESHI, AOKI TAKEO, HAGIWARA HARUO, Shigeyasu Tanaka, Eiki Kominami, TAKATA KUNIAKI
Anat Sci Int 77 ( 3 ) 189 2002
-
Activation of organelle DNA synthesis during the initial phase of proliferation of BY-2 cultured tobacco cells after medium renewal.
Saori Okamura, SUZUKI TAKESHI, Yutaka Miyazawa, Tsuneyoshi Kuroiwa, Atsushi Sakai
Plant Morphology 14,pp 16-28 ( 1 ) 16 - 28 2002
-
The apical localization of SGLT1 glucose transporter is determined by the short amino acid sequence in its N-terminal domain
T Suzuki, K Fujikura, H Koyama, T Matsuzaki, Y Takahashi, K Takata
EUROPEAN JOURNAL OF CELL BIOLOGY 80 ( 12 ) 765 - 774 2001.12
-
The apical localization of SGLT1 glucose transporter is determined by the short amino acid sequence in its N-terminal domain
T Suzuki, K Fujikura, H Koyama, T Matsuzaki, Y Takahashi, K Takata
EUROPEAN JOURNAL OF CELL BIOLOGY 80 ( 12 ) 765 - 774 2001.12
-
免疫組織化学の基礎的手順 : 抗体入手から検境まで
高田 邦昭, 松崎 利行, 鈴木 健史, 青木 武生, 萩原 治夫, 藤倉 恵子
組織細胞化学 2001 80 - 87 2001.7
-
B-1 Immunolocalization of Organic Cation Transporters, rOCT1 and rOCT2 in the Rat Kidney :
SUGAWARA-YOKOO Minako, MATSUZAKI Toshiyuki, SUZUKI Takeshi, TAKATA Kuniaki
Acta histochemica et cytochemica 34 ( 1 ) 70 - 70 2001
-
初期エンドソーム蛋白質EEA1と内在化カベオラの関連性
青木武生, 萩原治夫, 鈴木健史, 松崎利行, 高田邦昭
日本組織細胞化学会総会・学術集会講演プログラム・予稿集 42nd 2001
-
MDCK細胞における水チャネルタンパク質,アクアポリン3の高浸透圧刺激による発現変化
松崎利行, 鈴木健史, 高田邦昭
解剖学雑誌 76 ( 1 ) 2001
-
Glucose transporters - Localization to specific membrane domains.
TAKATA KUNIAKI, SUZUKI TAKESHI, MATSUZAKI TOSHIYUKI, Nomingerel Tserentsoodol, TAJIKA YUKI, Yasuo Shinoda, HAGIWARA HARUO, AOKI TAKEO
電子顕微鏡 36 supplement 2,pp 23-25 2001
-
Hypertonicity-induced expression of aquaporin 3 in MDCK cells
Toshiyuki Matsuzaki, Takeshi Suzuki, Kuniaki Takata
American Journal of Physiology - Cell Physiology 281 ( 1 ) C55 - C63 2001
-
Glucose transporters - Localization to specific membrane domains.
TAKATA KUNIAKI, SUZUKI TAKESHI, MATSUZAKI TOSHIYUKI, Nomingerel Tserentsoodol, TAJIKA YUKI, Yasuo Shinoda, HAGIWARA HARUO, AOKI TAKEO
電子顕微鏡 36 supplement 2,pp 23-25 2001
-
Expression of sugar transporters by in vivo electroporation and particle gun methods in the rat liver: Localization to specific membrane domains
Y Shinoda, T Suzuki, M Sugawara-Yokoo, S Nagamatsu, H Kuwano, K Takata
ACTA HISTOCHEMICA ET CYTOCHEMICA 34 ( 1 ) 15 - 24 2001
-
Expression of sugar transporters by in vivo electroporation and particle gun methods in the rat liver: Localization to specific membrane domains
Y Shinoda, T Suzuki, M Sugawara-Yokoo, S Nagamatsu, H Kuwano, K Takata
ACTA HISTOCHEMICA ET CYTOCHEMICA 34 ( 1 ) 15 - 24 2001
-
Hypertonicity-induced expression of aquaporin 3 in MDCK cells
MATSUZAKI T.
Am. J. Physiol. Cell Physiol. 281,pp C55-C63 C55 - C63 2001
-
S3-4 Intracellular Localization and Dynamics of Insulin-recruitable Glucose Transporter GLUT4 :
TAKATA Kuniaki, FUJIKURA Keiko, SHINODA Yasuko, SUZUKI Takeshi
Acta histochemica et cytochemica 34 ( 1 ) 47 - 47 2001
-
Immunohistochemical analysis of intracellular localization of insulin-sensitive glucose transporter GLUT4 in 3T3-L1 adipocytes
FUJIKURA K., SUZUKI M., SUZUKI T., SUGAWARA-YOKOO M., MATSUZAKI T., TAKATA K.
35 465 - 465 2000.5
-
上皮細胞におけるナトリウムイオン依存型糖輸送体SGLT1の頂部細胞膜局在機構．
鈴木 健史
北関東医学 50,pp 399-401 ( 4 ) 399 - 401 2000
-
糖輸送体SGLT1の頂部細胞膜移送経路の解析
鈴木健史, 小山晴子, 松崎利行, 高田邦昭
日本細胞生物学会大会講演要旨集 53rd 2000
-
ラット腎臓における促進拡散型糖輸送体GLUT4の局在
横尾美奈子, 鈴木健史, 松崎利行, 藤倉恵子, 成清卓二, 高田邦昭
解剖学雑誌 75 ( 1 ) 2000
-
Water channel protein, aquaporin 3, in epithelial cells
MATSUZAKI TOSHIYUKI, SUZUKI TAKESHI, TAKATA KUNIAKI
Kluwer Academic/Plenum Publishers, New York pp167-172 2000
-
Water channel protein, aquaporin 3, in epithelial cells
MATSUZAKI TOSHIYUKI, SUZUKI TAKESHI, TAKATA KUNIAKI
Kluwer Academic/Plenum Publishers, New York pp167-172 2000
-
An anti-peptide antibody that recognized unexpected protein - A case report
Toshiyuki Matsuzaki, Suzuki Takeshi, Shigeyasu Tanaka, Kuniaki Tanaka
Acta Histochemica et Cytochemica 33 ( 5 ) 361 - 365 2000
-
An anti-peptide antibody that recognized unexpected protein---A case report
MATSUZAKI TOSHIYUKI, SUZUKI TAKESHI, Tanaka S, TAKATA KUNIAKI
Acta Histochem Cytochem 33 ( 5 ) 361 - 365 2000
-
ラット唾液腺の水チャネル,アクアポリン5
松崎利行, 鈴木健史, 高田邦昭
解剖学雑誌 75 ( 1 ) 2000
-
ラット上皮組織およびMDCK細胞における水チャネルタンパク質,アクアポリン3
松崎利行, 鈴木健史, 田中滋康, 高田邦昭
解剖学雑誌 75 ( 5 ) 2000
-
糖輸送体SGLT1の発現調節系を用いた頂部細胞膜局在分布機構の解析
鈴木健史, 小山晴子, 松崎利行, 藤倉恵子, 高田邦昭
解剖学雑誌 75 ( 1 ) 2000
-
ラット腎臓における有機カチオントランスポーター(OCT1,OCT2)の分布局在
横尾美奈子, 松崎利行, 鈴木健史, 高田邦昭
日本組織細胞化学会総会・学術集会講演プログラム・予稿集 41st 2000
-
An anti-peptide antibody that recognized unexpected protein - A case report
T Matsuzaki, T Suzuki, S Tanaka, K Takata
ACTA HISTOCHEMICA ET CYTOCHEMICA 33 ( 5 ) 361 - 365 2000
-
An anti-peptide antibody that recognized unexpected protein - a case report.
MATSUZAKI TOSHIYUKI, SUZUKI TAKESHI, Shigeyasu Tanaka, TAKATA KUNIAKI
Acta Histochem Cytochem 33 ( 5 ) 361 - 365 2000
-
Immunolocalization of tight junction proteins, occludin and ZO-1, and glucose transporter GLUT1 in the cells of the blood-nerve barrier
N Tserentsoodol, BC Shin, H Koyama, T Suzuki, K Takata
ARCHIVES OF HISTOLOGY AND CYTOLOGY 62 ( 5 ) 459 - 469 1999.12
-
Immunolocalization of tight junction proteins, occludin and ZO-1, and glucose transporter GLUT1 in the cells of the blood-nerve barrier
N Tserentsoodol, BC Shin, H Koyama, T Suzuki, K Takata
ARCHIVES OF HISTOLOGY AND CYTOLOGY 62 ( 5 ) 459 - 469 1999.12
-
Laser confocal microscopic analysis of the localization of Na^+-dependent glucose transporter SGLT1
SUZUKI T., FUJIKURA K., KOYAMA H., MAGSHZAKI T., TAKATA K.
Electron-microscopy 34 105 - 108 1999.11
-
Water channel protein AQP3 is present in epithelia exposed to the environment of possible water loss
T Matsuzaki, T Suzuki, H Koyama, S Tanaka, K Takata
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY 47 ( 10 ) 1275 - 1286 1999.10
-
Water channel protein AQP3 is present in epithelia exposed to the environment of possible water loss
T Matsuzaki, T Suzuki, H Koyama, S Tanaka, K Takata
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY 47 ( 10 ) 1275 - 1286 1999.10
-
Presence of fructose transporter GLUT5 in the S3 proximal tubules in the rat kidney
M Sugawara-Yokoo, T Suzuki, T Matsuzaki, T Naruse, K Takata
KIDNEY INTERNATIONAL 56 ( 3 ) 1022 - 1028 1999.9
-
Presence of fructose transporter GLUT5 in the S3 proximal tubules in the rat kidney
M Sugawara-Yokoo, T Suzuki, T Matsuzaki, T Naruse, K Takata
KIDNEY INTERNATIONAL 56 ( 3 ) 1022 - 1028 1999.9
-
Peptide transporter in the rat small intestine: Ultrastructural localization and the effect of starvation and administration of amino acids
H Ogihara, T Suzuki, Y Nagamachi, K Inui, K Takata
HISTOCHEMICAL JOURNAL 31 ( 3 ) 169 - 174 1999.3
-
Peptide transporter in the rat small intestine: Ultrastructural localization and the effect of starvation and administration of amino acids
H Ogihara, T Suzuki, Y Nagamachi, K Inui, K Takata
HISTOCHEMICAL JOURNAL 31 ( 3 ) 169 - 174 1999.3
-
Peptide transporter in the rat small intestine: Ultrastructural localization and the effect of starvation and administration of amino acids
H Ogihara, T Suzuki, Y Nagamachi, K Inui, K Takata
HISTOCHEMICAL JOURNAL 31 ( 3 ) 169 - 174 1999.3
-
Aquaporin-5 (AQP5), a water channel protein, in the rat salivary and lacrimal glands: immunolocalization and effect of secretory stimulation
T Matsuzaki, T Suzuki, H Koyama, S Tanaka, K Takata
CELL AND TISSUE RESEARCH 295 ( 3 ) 513 - 521 1999.3
-
Aquaporin-5 (AQP5), a water channel protein, in the rat salivary and lacrimal glands: immunolocalization and effect of secretory stimulation
T Matsuzaki, T Suzuki, H Koyama, S Tanaka, K Takata
CELL AND TISSUE RESEARCH 295 ( 3 ) 513 - 521 1999.3
-
Presence of fructose transporter GLUT5 in the S3 proximal tubules in the rat kidney
Minako Sugawara-Yokoo, Takeshi Suzuki, Toshiyuki Matsuzaki, Takuji Naruse, Kuniaki Takata
Kidney International 56 ( 3 ) 1022 - 1028 1999
-
ラット泌尿器系における水チャネル,アクアポリン3の局在
松崎利行, 鈴木健史, 高田邦昭
日本組織細胞化学会総会・学術集会講演プログラム・予稿集 40th 1999
-
ラット上皮組織における水チャネル,アクアポリン3
松崎利行, 鈴木健史, 小山晴子, 田中滋康, 高田邦昭
日本細胞生物学会大会講演要旨集 52nd 1999
-
Immunolocalization and effect of secretory stimulation
MATSUZAKI TOSHIYUKI, SUZUKI TAKESHI, TAKATA KUNIAKI, Koyama H, Tanaka S
Cell Tissue Res 295,pp513-521 1999
-
Aquaporin-5 (AQP5), a water channel protein, in the rat salivary and lacrimal gland : Immunolocalization and effect of secretory stimulation.
MATSUZAKI TOSHIYUKI, SUZUKI TAKESHI, Koyama H, Tanaka S, TAKATA KUNIAKI
Cell Tissue Res 295,pp513-521 1999
-
Immunolocalization and effect of secretory stimulation
MATSUZAKI TOSHIYUKI, SUZUKI TAKESHI, TAKATA KUNIAKI, Koyama H, Tanaka S
Cell Tissue Res 295,pp513-521 1999
-
Sugar transporters in polarized epithelial cells
Kuniaki Takata, Akiko Iizuka, Takeshi Suzuki, Toshiyuki Matsuzaki, Minako Sugawara, Bo-Chul Shin, Tserentsoodol Nomingerel, Yasuo Shinoda
Acta Histochemica et Cytochemica 32 ( 1 ) 53 - 58 1999
-
Sugar transporters in polarized epithelial cells.
TAKATA KUNIAKI, Akiko Iizuka, SUZUKI TAKESHI, MATSUZAKI TOSHIYUKI, YOKOO MINAKO, Bo-Chul Shin, Tserentsoodol Nomingerel, Yasuo Shinoda
Acta Histochem Cytochem 32 ( 1 ) 53 - 58 1999
-
Sugar transporters in polarized epithelial cells
K Takata, A Iizuka, T Suzuki, T Matsuzaki, M Sugawara, BC Shin, T Nomingerel, Y Shinoda
ACTA HISTOCHEMICA ET CYTOCHEMICA 32 ( 1 ) 53 - 58 1999
-
Peptide transporter in the rat small intestine: Ultrastructural localization and the effect of starvation and administration of amino acids
Hiroshi Ogihara, Takeshi Suzuki, Yukio Nagamachi, Ken-Ichi Inui, Kuniaki Takata
Histochemical Journal 31 ( 3 ) 169 - 174 1999
-
Water channel protein AQP3 is present in epithelia exposed to the environment of possible water loss
Toshiyuki Matsuzaki, Takeshi Suzuki, Haruko Koyama, Shigeyasu Tanaka, Kuniaki Takata
Journal of Histochemistry and Cytochemistry 47 ( 10 ) 1275 - 1286 1999
-
鈴木 健史, 高田 邦昭
日本組織細胞化学会総会プログラムおよび抄録集 ( 40 ) 91 - 91 1999
-
Plasma membrane localizization mechanism of the glucose transporter SGLT1 :
Suzuki Takeshi, Takata Kuniaki
Acta histochemica et cytochemica 32 ( 6 ) 504 - 504 1999
-
Immunolocalization of water channel protein, aquaporin 3, in the rat urinary system :
MATSUZAKI Toshiyuki, SUZUKI Takeshi, TAKATA Kuniaki
Acta histochemica et cytochemica 32 ( 6 ) 546 - 546 1999
-
Multiple isoforms of the regulatory subunit for phosphatidylinositol 3-kinase (PI3-kinase) are expressed in neurons in the rat brain
Bo-Chul Shin, Masakazu Suzuki, Kouichi Inukai, Motonobu Anai, Tomoichiro Asano, Kuniaki Takata
Biochemical and Biophysical Research Communications 246 ( 2 ) 313 - 319 1998.5
-
Direct gene transfer into rat liver cells by in vivo electroporation
Takeshi Suzuki, Bo-Chul Shin, Keiko Fujikura, Toshiyuki Matsuzaki, Kuniaki Takata
FEBS Letters 425 ( 3 ) 436 - 440 1998.4
-
Direct gene transfer into rat liver cells by in vivo electroporation
T Suzuki, BC Shin, K Fujikura, T Matsuzaki, K Takata
FEBS LETTERS 425 ( 3 ) 436 - 440 1998.4
-
Direct gene transfer into rat liver cells by in vivo electroporation
T Suzuki, BC Shin, K Fujikura, T Matsuzaki, K Takata
FEBS LETTERS 425 ( 3 ) 436 - 440 1998.4
-
luorescence counter-staining of cell nuclear DNA for multi-color laser confocal microscopy.
SUZUKI TAKESHI, MATSUZAKI TOSHIYUKI, TAKATA KUNIAKI
Acta Histochem Cytochem 31 ( 4 ) 297 - 301 1998
-
Fluorescence counter-staining of cell nuclear DNA for multi-color laser confocal microscopy
T Suzuki, T Matsuzaki, K Takata
ACTA HISTOCHEMICA ET CYTOCHEMICA 31 ( 4 ) 297 - 301 1998
-
Fluorescence counter-staining of cell nuclear DNA for multi-color laser confocal microscopy
SUZUKI TAKESHI, MATSUZAKI TOSHIYUKI, TAKATA KUNIAKI
Acta Histochem Cytochem 31 ( 4 ) 297 - 301 1998
-
luorescence counter-staining of cell nuclear DNA for multi-color laser confocal microscopy.
SUZUKI TAKESHI, MATSUZAKI TOSHIYUKI, TAKATA KUNIAKI
Acta Histochem Cytochem 31 ( 4 ) 297 - 301 1998
-
上皮細胞における細胞膜糖輸送体の局在機構．
高田 邦昭, 鈴木 健史, 飯塚 昌子, 藤倉 恵子, 松崎 利行, 小山 晴子, 犬飼 浩一, 浅野 知一郎
電子顕微鏡 33 supplement 2,pp 157-160 1998
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ラット胎盤の糖輸送機構．
高田 邦昭, Bo-Chul Shin, 藤倉 恵子, 鈴木 雅一, 鈴木 健史
Mebio 15(No.7),pp 95-97 ( 7 ) 1998
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乳腺の糖輸送体．
高田 邦昭, 藤倉 恵子, 鈴木 雅一, 鈴木 健史
Mebio 15(No.5),pp 91-93 1998
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高田 邦昭, 鈴木 健史, 飯塚 晶子
電子顕微鏡 33 ( 2 ) 157 - 160 1998
-
Multiple isoforms of the regulatory subunit for phosphatidylinositol 3-kinase(PI3-kinase)are expressed in neurons in the rat brain.
Shin B-C, SUZUKI TAKESHI, Inukai K, Anai M, Asano T, TAKATA KUNIAKI
Biochem Biophys Res Commun 246 ( 2 ) 313 - 319 1998
-
Multiple isoforms of the regulatory subunit for phosphatidylinositol 3-kinase (PI3-kinase) are expressed in neurons in the rat brain.
Shin B-C, SUZUKI TAKESHI, Inukai K, Anai M, Asano T, TAKATA KUNIAKI
Biochem Biophys Res Commun 246 ( 2 ) 313 - 319 1998
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Multiple isoforms of the regulatory subunit for phosphatidylinositol 3-kinase(PI3-kinase)are expressed in neurons in the rat brain.
Shin B-C, SUZUKI TAKESHI, Inukai K, Anai M, Asano T, TAKATA KUNIAKI
Biochem Biophys Res Commun 246 ( 2 ) 313 - 319 1998
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Sugar transporter and connexin Involvement in transepithelial transport in the epithelium of bilayers.
高田邦昭, SHIN B-C, 藤倉恵子, 鈴木健史, 松崎利行, 田中滋康
日本動物学会大会予稿集 69th 1998
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ラット胎盤の糖輸送機構
高田 邦昭, Shin B-C, 藤倉 恵子, 鈴木 健史, 松崎 利行
Mebio 15(7),pp95-97 ( 7 ) 1998
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上皮細胞における細胞膜糖輸送体の局在機構
高田 邦昭, 鈴木 健史, 飯塚 晶子, 藤倉 恵子, 松崎 利行, 小山 晴子, 犬飼 浩一, 浅野 知一郎
電子顕微鏡 33 (suppleemnt 2),pp157-160 1998
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Fluorescence counter-staining of cell nuclear DNA for multi-color laser confocal microscopy
SUZUKI TAKESHI, MATSUZAKI TOSHIYUKI, TAKATA KUNIAKI
Acta Histochem Cytochem 31 ( 4 ) 297 - 301 1998
-
Subcellular distribution of GLUT4 in Chinese hamster ovary cells overexpressing mutant dynamin: Evidence that dynamin is a regulatory GTPase in GLUT4 endocytosis
Waka Omata, Hiroshi Shibata, Yoichi Suzuki, Shigeyasu Tanaka, Takeshi Suzuki, Kuniaki Takata, Itaru Kojima
Biochemical and Biophysical Research Communications 241 ( 2 ) 401 - 406 1997.12
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Subcellular distribution of GLUT4 in Chinese hamster ovary cells overexpressing mutant dynamin: Evidence that dynamin is a regulatory GTPase in GLUT4 endocytosis
W Omata, H Shibata, Y Suzuki, S Tanaka, T Suzuki, K Takata, Kojima, I
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 241 ( 2 ) 401 - 406 1997.12
-
Kir6.1: A possible subunit of ATP-sensitive K+ channels in mitochondria
M Suzuki, K Kotake, K Fujikura, N Inagaki, T Suzuki, T Gonoi, S Seino, K Takata
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 241 ( 3 ) 693 - 697 1997.12
-
Kir6.1: A possible subunit of ATP-sensitive K+ channels in mitochondria
M Suzuki, K Kotake, K Fujikura, N Inagaki, T Suzuki, T Gonoi, S Seino, K Takata
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 241 ( 3 ) 693 - 697 1997.12
-
Glucose transporter GLUT3 in the rat placental barrier: A possible machinery for the transplacental transfer of glucose
BC Shin, K Fujikura, T Suzuki, S Tanaka, K Takata
ENDOCRINOLOGY 138 ( 9 ) 3997 - 4004 1997.9
-
Glucose transporter GLUT3 in the rat placental barrier: A possible machinery for the transplacental transfer of glucose
BC Shin, K Fujikura, T Suzuki, S Tanaka, K Takata
ENDOCRINOLOGY 138 ( 9 ) 3997 - 4004 1997.9
-
DNA staining for fluorescence and laser confocal microscopy
T Suzuki, K Fujikura, T Higashiyama, K Takata
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY 45 ( 1 ) 49 - 53 1997.1
-
DNA staining for fluorescence and laser confocal microscopy
Takeshi Suzuki, Keiko Fujikura, Tetsuya Higashiyama, Kuniaki Takata
Journal of Histochemistry and Cytochemistry 45 ( 1 ) 49 - 53 1997
-
共焦点レーザー顕微鏡のための核染色色素の検討
松崎利行, 鈴木健史, 高田邦昭
日本組織細胞化学会総会・学術集会講演プログラム・予稿集 38th 1997
-
Fluorescent antibody staining and nucleic acid contrast staining in laser confocal microscope method.
鈴木健史, 松崎利行, 高田邦昭
日本比較内分泌学会ニュース ( 87 ) 1997
-
GLUT1 glucose transporter in the lactating mammary gland in the rat
K Takata, K Fujikura, M Suzuki, T Suzuki, H Hirano
ACTA HISTOCHEMICA ET CYTOCHEMICA 30 ( 5-6 ) 623 - 628 1997
-
Nuclear staining for laser confocal microscopy
Toshiyuki Matsuzaki, Takeshi Suzuki, Keiko Fujikura, Kuniaki Takata
Acta Histochemica et Cytochemica 30 ( 3 ) 309 - 314 1997
-
Nuclear staining for laser confocal microscopy
T Matsuzaki, T Suzuki, K Fujikura, K Takata
ACTA HISTOCHEMICA ET CYTOCHEMICA 30 ( 3 ) 309 - 314 1997
-
Structural basis of sugar transport across the placental barrier
TAKATA KUNIAKI, Shin B-C, MATSUZAKI TOSHIYUKI, SUZUKI TAKESHI, Tanaka S, Fujikura K
J Reprod Develop 43 ( Supplement ) 53 - 54 1997
-
Structural basis of sugar transport across the placental barrier
TAKATA KUNIAKI, Shin B-C, MATSUZAKI TOSHIYUKI, SUZUKI TAKESHI, Tanaka S, Fujikura K
J Reprod Develop 43 ( Supplement ) 53 - 54 1997
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Na+-dependent glucose transporter SGLT1 is localized in the apical plasma membrane upon completion of tight junction formation in MDCK cells
T Suzuki, K Fujikura, K Takata
HISTOCHEMISTRY AND CELL BIOLOGY 106 ( 6 ) 529 - 533 1996.12
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Na+-dependent glucose transporter SGLT1 is localized in the apical plasma membrane upon completion of tight junction formation in MDCK cells
T Suzuki, K Fujikura, K Takata
HISTOCHEMISTRY AND CELL BIOLOGY 106 ( 6 ) 529 - 533 1996.12
-
Variability of mitochondrial subgenomic molecules in the meristematic cells of higher plants
T Suzuki, S Kawano, A Sakai, A Hirai, T Kuroiwa
GENES & GENETIC SYSTEMS 71 ( 5 ) 329 - 333 1996.10
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Variability of mitochondrial subgenomic molecules in the meristematic cells of higher plants
T Suzuki, S Kawano, A Sakai, A Hirai, T Kuroiwa
GENES & GENETIC SYSTEMS 71 ( 5 ) 329 - 333 1996.10
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種子休眠打破による3ゲノムDNA合成のダイナミクス
林 誠, 鈴木 健史, 黒岩 晴子, 黒岩 常祥, 桜井 成
日本植物学会大会研究発表記録 = Proceedings of the annual meeting of the Botanical Society of Japan 60 292 - 292 1996.10
-
Connexin 43 and the glucose transporter, GLUT1, in the ciliary body of the rat
BC Shin, T Suzuki, S Tanaka, A Kuraoka, Y Shibata, K Takata
HISTOCHEMISTRY AND CELL BIOLOGY 106 ( 2 ) 209 - 214 1996.8
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Connexin 43 and the glucose transporter, GLUT1, in the ciliary body of the rat
BC Shin, T Suzuki, S Tanaka, A Kuraoka, Y Shibata, K Takata
HISTOCHEMISTRY AND CELL BIOLOGY 106 ( 2 ) 209 - 214 1996.8
-
Immunolocalization of GLUT1 and connexin 26 in the rat placenta
BC Shin, T Suzuki, T Matsuzaki, S Tanaka, A Kuraoka, Y Shibata, K Takata
CELL AND TISSUE RESEARCH 285 ( 1 ) 83 - 89 1996.7
-
Immunolocalization of GLUT1 and connexin 26 in the rat placenta
BC Shin, T Suzuki, T Matsuzaki, S Tanaka, A Kuraoka, Y Shibata, K Takata
CELL AND TISSUE RESEARCH 285 ( 1 ) 83 - 89 1996.7
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蛍光抗体法
高田邦昭, Shin Bo-Chul, 鈴木 健史, 藤倉 恵子
組織細胞化学1996 107 - 113 1996
-
Organelle DNA synthesis before cell nuclear replication is essential for subsequent cell propagation
Takeshi Suzuki, Atsushi Sakai, Shigeyuki Kawano, Tsuneyoshi Kuroiwa
Cytologia 61 ( 2 ) 235 - 245 1996
-
胎盤と物質輸送
高田 邦昭, Bo-Chul Shin, 藤倉 恵子, 田中 滋康, 松崎 利行, 鈴木 健史
産科と婦人科 63,pp1347-1352 1996
-
Na<sup>+</sup>-dependent glucose transporter SGLT1 is localized in the apical plasma membrane upon completion of tight junction formation in MDCK cells.
SUZUKI TAKESHI, Fujikura K, TAKATA KUNIAKI
Histochem Cell Biol 106,pp529-533 1996
-
Na+-dependent glucose transporter SGLT1 is localized in the apical plasma membrane upon completion of tight junction formation in MDCK cells
Takeshi Suzuki, Keiko Fujikura, Kuniaki Takata
Histochemistry and Cell Biology 106 ( 6 ) 529 - 533 1996
-
胎盤と物質輸送．
高田 邦昭, Bo-Chul Shin, 藤倉 恵子, 田中 滋康, 松崎 利行, 鈴木 健史
産科と婦人科 63,pp 1347-1352 1996
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LOCALIZATION OF ORGANELLE DNA-SYNTHESIS WITHIN THE ROOT APICAL MERISTEM OF RICE
T SUZUKI, N SASAKI, A SAKAI, S KAWANO, T KUROIWA
JOURNAL OF EXPERIMENTAL BOTANY 46 ( 282 ) 19 - 25 1995.1
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LOCALIZATION OF ORGANELLE DNA-SYNTHESIS WITHIN THE ROOT APICAL MERISTEM OF RICE
T SUZUKI, N SASAKI, A SAKAI, S KAWANO, T KUROIWA
JOURNAL OF EXPERIMENTAL BOTANY 46 ( 282 ) 19 - 25 1995.1
-
Gap junction and sugar transport.
高田邦昭, SHIN B-C, 鈴木健史, 松崎利行
Mebio 12(No.11),pp 149-151 ( 11 ) 1995
-
PERMEATION OF HYDROGEN IN PALLADIUM
K TAKATA, T SUZUKI
MATERIALS TRANSACTIONS JIM 34 ( 1 ) 89 - 91 1993.1
-
PREFERENTIAL MITOCHONDRIAL AND PLASTID DNA-SYNTHESIS BEFORE MULTIPLE CELL DIVISIONS IN NICOTIANA-TABACUM
T SUZUKI, S KAWANO, A SAKAI, M FUJIE, H KUROIWA, H NAKAMURA, T KUROIWA
JOURNAL OF CELL SCIENCE 103 831 - 837 1992.11
-
PREFERENTIAL MITOCHONDRIAL AND PLASTID DNA-SYNTHESIS BEFORE MULTIPLE CELL DIVISIONS IN NICOTIANA-TABACUM
T SUZUKI, S KAWANO, A SAKAI, M FUJIE, H KUROIWA, H NAKAMURA, T KUROIWA
JOURNAL OF CELL SCIENCE 103 831 - 837 1992.11
-
組織細胞内におけるオルガネラDNA合成部位の検出法 -テクノビット-BrdU抗体蛍光抗体染色法-．
鈴木 健史
植物細胞工学 4,pp 416-417 416 - 417 1992
-
オルガネラゲノムからみた細胞の増殖 -細胞周期はあるか-．
鈴木 健史, 黒岩 常祥
植物細胞工学 4,pp 405-415 1992
-
THE RICE MITOCHONDRIAL NAD3 GENE HAS AN EXTENDED READING FRAME AT ITS 5' END - NUCLEOTIDE-SEQUENCE ANALYSIS OF RICE TRNS, NAD3, AND RPS12 GENES
T SUZUKI, S KAZAMA, A HIRAI, T AKIHAMA, K KADOWAKI
CURRENT GENETICS 20 ( 4 ) 331 - 337 1991
-
THE RICE MITOCHONDRIAL NAD3 GENE HAS AN EXTENDED READING FRAME AT ITS 5' END - NUCLEOTIDE-SEQUENCE ANALYSIS OF RICE TRNS, NAD3, AND RPS12 GENES
T SUZUKI, S KAZAMA, A HIRAI, T AKIHAMA, K KADOWAKI
CURRENT GENETICS 20 ( 4 ) 331 - 337 1991
Presentations
-
免疫シナプスにおけるシグナル分子動態
日本顕微鏡学会 2009
-
蛍光タンパク質の基礎と実際
組織細胞化学講習会 2008
-
共焦点顕微鏡の基礎と応用；デコンボリューション顕微鏡法による細胞機能解析
分析電子顕微鏡討論会 2008
-
Water channel protein, aquaporin 3, in epithelial cells.
3rd international conference on molecular biology and physiology of water and solute transport, Göteborg 2000
-
Water channel protein aquaporin 3 (AQP3): immunolocalization in epithelial cells and effect of hypertonicity.
Fifteenth international symposium on morphological sciences 2000
-
水チャネル、アクアポリン3のラット上皮組織における局在．（ミニシンポジウム）
第104回日本解剖学会総会・全国学術集会，杏林大学三鷹キャンパス 1999
-
AQP5水チャネルのラット唾液腺における局在．
日本解剖学会第86回関東地方会，東京女子医科大学医学部 1999
-
Immunocytochemical analysis of intracellular pool of water channel aquaporin-2 (AQP2) in renal epithelial cells
World Congress on Neurohypophysial Hormones 2003
-
ラット上皮組織における水チャネル、アクアポリン3．
第52回日本細胞生物学会大会，東京大学本郷キャンパス 1999
-
共焦点レーザー顕微鏡および蛍光顕微鏡を使いこなすための基礎知識
鈴木 健史
第35回組織細胞化学講習会 2011.8
-
ラット上皮組織およびMDCK細胞における水チャネルタンパク質，アクアポリン3．
日本解剖学会第87回関東地方会，群馬大学医学部 1999
-
ラット泌尿器系における水チャネル、アクアポリン3の局在．
日本組織細胞化学会第40回記念総会・学術集会，国立京都国際会館 1999
-
Immunolocalization of the water channel AQP5 in rat salivary glands.
Fifth Joint Meeting of the Japan Society of Histochemistry and Cytochemistry and Histochemical Society, University of California, San Diego, La Jolla 1998
-
Immunolocalization of the water channel AQP5 in rat salivary glands.
Fifth Joint Meeting of the Japan Society of Histochemistry and Cytochemistry and Histochemical Society, University of California, San Diego, La Jolla 1998
-
共焦点レーザー顕微鏡のための核染色色素の検討．
第38回日本組織細胞化学会総会・学術集会，三鷹市芸術文化センター 1997
-
細胞核染色蛍光色素 — 共焦点レーザー顕微鏡における検討．
日本解剖学会第85回関東地方会，北里大学医学部 1997
-
ミオ−イノシトール輸送体SMITの局在．
日本解剖学会第83回関東地方会，杏林大学医学部 1995
-
ゴルジ装置構成たんぱく質の中心体への局在について
第１１０回日本解剖学会総会・全国学術集会 2005
-
Aquaporins in the rat nasal mucosa.
第46回日本組織細胞化学会総会・学術集会 2005
-
Targeting of PKC-theta at cSMAC during T-cell activation is regulate by its v3 domain other than C1-DAG binding machinery.
The 58th Annual Meeting of Japan Society for Cell Biology 2005
-
Three-dimensional time-lapse-imaging for signal molecules during T-cell activation. (Prize-winning lecture for the JSHC Young Investigator of the Year)
7th Joint Meeting of The Histochemical Society & The Japan Society of Histochemistry and Cytochemistry 2006
-
Three-dimensional time-lapse-imaging for signal molecules during T-cell activation
7th Joint Meeting of The Histochemical Society & The Japan Society of Histochemistry and Cytochemistry 2006
-
Immunohistochemical study of Golgi proteins to the centrosome
16th International Microscopy Congress 2006
-
Aquaporins in the rat nasal mucosa.
第46回日本組織細胞化学会総会・学術集会 2005
-
Targeting of PKC-theta at cSMAC during T-cell activation is regulate by its v3 domain other than C1-DAG binding machinery.
The 58th Annual Meeting of Japan Society for Cell Biology 2005
-
デコンボリューション顕微鏡法。
日本顕微鏡学会第61回学術講演会 2005
-
内在化されたカベオラは初期エンドソームで選別されている
第110回日本解剖学会・全国学術集会 2005
-
Role of early endosomes in caevolar internalization in endothelial cells
16th International Congress of the IFAA 2004
-
Role of the action microtubule cytoskelton in the intracellular distribution of AQP2.
16th International Congress of the IFAA 2004
-
ラット乳腺における水チャネルAQP1およびAQP3の局在
第４５回日本組織細胞化学会総会・学術集会 2004
-
Mapping of aquaporins in the body
16th International Congress of the International Federation of Associations of Anatomists (IFAA) 2004
-
授乳期ラット乳腺におけるアクアポリン水チャネルの分布局在
日本解剖学会関東支部 第92回学術集会 2004
-
Mapping of aquaporins in the body
16th International Congress of the International Federation of Associations of Anatomists (IFAA) 2004
-
Role of early endosomes in caevolar internalization in endothelial cells
16th International Congress of the IFAA 2004
-
Role of the action microtubule cytoskelton in the intracellular distribution of AQP2.
16th International Congress of the IFAA 2004
-
カベオラの内在化と膜動態
第108回日本解剖学会総会・全国学術集会 2003
-
Water channel protein aquaporin 3 (AQP3): immunolocalization in epithelial cells and effect of hypertonicity.
Fifteenth international symposium on morphological sciences 2000
-
Immunocytochemical analysis of intracellular pool of water channel aquaporin-2 (AQP2) in renal epithelial cells
World Congress on Neurohypophysial Hormones 2003
-
ナトリウムイオン依存性糖輸送体SGLT1の頂部細胞膜局在に対するコレステロール除去の影響
日本解剖学会総会・全国学術集会 2002
-
カベオラは内在化されたあとエンドソームに運ばれるか？
日本解剖学会第89回関東支部学術集会 2001
-
初期エンドソーム蛋白質EEA1と内在化カベオラとの関連性
日本組織細胞化学会第42回総会・学術集会 2001
-
消化管での糖吸収機構の形態学
日本解剖学会総会・学術集会 2001
-
MDCK細胞における水チャネルタンパク質、アクアポリン3の高浸透圧刺激による発現変化
第106回日本解剖学会総会・全国学術集会 2001
-
ラット唾液腺の水チャネル、アクアポリン5．（シンポジウム）
第105回日本解剖学会総会・全国学術集会，パシフィコ横浜 2000
-
Water channel protein, aquaporin 3, in epithelial cells.
3rd international conference on molecular biology and physiology of water and solute transport, Göteborg 2000
-
免疫シナプス超分子クラスターの分子機能動態
日本組織細胞化学会 2007
-
Live-cell-imaging of signal molecules during the formation of immunological synapses.
Dairen University 2007
-
Live-cell-imaging of signal molecules during the formation of immunological synapses.
Dairen University 2007
-
Three-dimensional time-lapse-imaging for signal molecules during T-cell activation. (Prize-winning lecture for the JSHC Young Investigator of the Year)
7th Joint Meeting of The Histochemical Society & The Japan Society of Histochemistry and Cytochemistry 2006
-
Three-dimensional time-lapse-imaging for signal molecules during T-cell activation
7th Joint Meeting of The Histochemical Society & The Japan Society of Histochemistry and Cytochemistry 2006
-
Immunohistochemical study of Golgi proteins to the centrosome
16th International Microscopy Congress 2006
-
アクアポリン2水チャネルの細胞内貯蔵部位と細胞内動態
第111回日本解剖学会総会・全国学術集会 2006
-
3次元ライブセルイメージングによる免疫シナプスのシグナル分子動態の解析
第111回日本解剖学会総会・全国学術集会 2006
-
免疫シナプスにおけるシグナル分子分布動態の３次元ライブセルイメージング解析
日本顕微鏡学会 2007
-
アクアポリン2水チャネルのトラフィッキング機構と膜微小ドメイン
第111回日本解剖学会総会・全国学術集会 2006
-
免疫組織化学：どの方法を選ぶか
日本組織細胞化学会第28回組織細胞化学講習会 2003
-
バイオイメージングで知っておきたい顕微鏡の基礎
鈴木 健史
第35回組織細胞化学講習会，シンポジウム「見る！ 顕微鏡の基礎から最新のイメージング技術」 2012.8
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免疫シナプスにおけるシグナル分子動態
鈴木 健史
第52回組織細胞化学会･学術集会 2011.9
Industrial property rights
-
温水循環式顕微培養チャンバー
鈴木 健史
-
顕微鏡装置及びそれを用いた蛍光観察方法
五十嵐康伸, 出口雄規, 鈴木健史, 橋本浩一
Awards
-
ベストティーチャー賞
2013.3 札幌医科大学
鈴木 健史
-
ベストティーチャー優秀賞
2011.5 群馬大学
鈴木 健史
-
ベストティーチャー石井賞
2010.12 群馬大学医学部
鈴木 健史
-
日本組織細胞化学会論文賞
2006
-
JSHC Young Investigator Award
2006
-
JSHC Young Investigator Award
2006
-
日本組織細胞化学会論文賞
2003
-
Young Histochemist Award
2000
-
Young Histochemist Award
2000
Research Projects
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低体温時における免疫細胞の自己発熱による細胞活動性賦活化機構
Grant number：24K12136 2024.4 - 2027.3
日本学術振興会 科学研究費助成事業 基盤研究(C)
鈴木 健史, 井上 弘行, 成松 英智
Grant amount：\4680000 （ Direct Cost: \3600000 、 Indirect Cost：\1080000 ）
-
The mechanism of elongation of primary cilia from basal bodies in ciliogenesis
Grant number：24590261 2012 - 2014
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
HAGIWARA Haruo, SUZUKI Takeshi, ARISAWA Kenjiro, ASANO Anshin, NAKAKURA Takashi
Authorship：Collaborating Investigator(s) (not designated on Grant-in-Aid) Grant type：Competitive
Grant amount：\5200000 （ Direct Cost: \4000000 、 Indirect Cost：\1200000 ）
Primary cilia, an organelle found on nearly every cell in the human body, contain an axoneme, which is composed of microtubules and their associated structures. Lithium ion is known to promote the elongation of primary cilia in a variety of cell types, but it is unknown whether lithium is involved in the acetylation of alpha-tubulin in cilia. Because the acetylation of alpha-tubulin may be important for the elongation of primary cilia, in this study we examined the effects of lithium chloride (LiCl) treatments on the acetylation of alpha-tubulin and length of primary cilia in human fibroblast KD cells. We also investigated the involvement of alphaTAT1 (alpha-tubulin N-acetyltransferase 1) in the signaling pathway mediating glycogen synthase kinase-3beta(GSK-3beta) and adenylate cyclase III. Our results suggested that LiCl treatments activate alphaTAT1 by the inhibition of GSK-3 beta and promote the alpha-tubulin acetylation, and then elongate the primary cilia.
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Analysis of endosome/post-endosome system with special reference to water channel aquaporin 2
Grant number：18390057 2006 - 2009
Ministry of Education, Culture, Sports, Science and Technology Grants-in-Aid for Scientific Research(基盤研究(B)) 基盤研究(B)
Kuniaki TAKATA, 鈴木 健史, 萩原 治夫, 青木 武生, 松崎 利行, Takeshi SUZUKI, TOSHIYUKI MATSUZAKI, Haruo HAGIWARA, Takeo AOKI
Authorship：Collaborating Investigator(s) (not designated on Grant-in-Aid) Grant type：Competitive
Grant amount：\15950000 （ Direct Cost: \13700000 、 Indirect Cost：\2250000 ）
Vesicular trafficking that determines the number of cell surface molecules serves as one of important regulatory mechanisms found in the control of the reabsorption of water in the kidney, maintenance of blood glucose level, etc.Intracellular vesicles involved in the endocytosis and subsequent recycling process, such as endosomes and post-endosomal vesicles are key components in such vesicular trafficking and storage. We used water channel aquaporin 2 whose trafficking is regulated by hypophysial hormone vasopressin. Glucose transporter 4(GLUT4), whose trafficking is regulated by insulin in adipocytes and muscle cells, was coexpressed with aquaporin 2 in MDCK cells, and their localization was visualized by immunofluorescence and GFP-tagging methods. Aquaporin 2 and GLUT4 exhibited distinct localization pattern each other in both the resting and stimulated states, showing that aquaporin 2 and GLUT4 are stored in distinct post-endosomal compartments each other, and that their trafficking is differentially regulated. Translocation of aquaporin 2 has been considered to be regulated by its phosphorylation. We raised antibodies that specifically detect phosphorylated aquaporin 2. By western blotting and immunofluorescence microscopy, phosphorylation of aquaporin 2 was found not only on the cell surface but also intracellular vesicles, suggesting the ubiquitous presence of phosphorylated aquaporin 2.
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ライブセルイメージングによる上皮細胞の細胞極性形成機構の解析
Grant number：16689006 2004 - 2006
文部科学省 科学研究費補助金(若手研究(A)) 若手研究(A)
鈴木 健史
Authorship：Principal investigator Grant type：Competitive
Grant amount：\27690000 （ Direct Cost: \21300000 、 Indirect Cost：\6390000 ）
細胞極性構築過程における膜タンパク質の局在動態を3次元ライブセルイメージングにより解析した.タイトジャンクションフレームワークの構築過程におけるSGLTの分子局在動態をライブセル解析したところ,細胞極性のない状態の細胞で細胞膜全周に発現しているSGLT分子が,タイトジャンクションの完成にともない頂部細胞膜に徐々に濃縮され最終的に頂部細胞膜に限局した局在にシフトしていく様子を,同一の細胞で初めて確認できた.このSGLTの頂部細胞膜への移行や選択的局在に膜脂質が関与している可能性について検討した.コレステロールを急激に除去すると30分でSGLTが細胞膜全周に移行した.この際,タイトジャンクションに変化はなくコレステロールの過剰投与によって相殺されることから,SGLTの頂部細胞膜局在にコレステロールが重要であることを明らかにした.また,コルセミド添加により微小管を脱重合させた場合もSGLTの細胞膜全周への移行が起こることを発見した.この際もタイトジャンクションに変化はなく,上皮細胞シートの電気抵抗性や,他の頂部細胞膜マーカータンパクの染色性に変化がないことから,細胞間接着が壊れた結果の側方拡散による細胞膜全周への移行ではないことを確認した.培地中のコルセミドを洗い流し,通常の培地で培養すると微小管系の復元にともない徐々にSGLTの頂部細胞膜局在が復活する様子も確認した.以上の結果は,微小管系がSGLTの頂部細胞膜局在の維持に機能していることを示している.また,細胞極性は隣接する細胞との接触が引き金となって形成されるが,この細胞接触面にPIP3が一過的に出現することを見いだしている.また,免疫細胞の細胞極性形成過程におけるシグナル分子の動態もライブセルイメージングにより解析し,免疫シナプスにPKCやPIP3,DAGなどの様々なシグナル分子が集合する様子をとらえることに成功した.
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ライブセルイメージングによる上皮細胞の細胞極性形成機構の解析
2004 - 2006
Grant type：Competitive
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ライブセルイメージングによる上皮細胞の細胞極性形成機構の解析
2004 - 2006
Grant type：Competitive
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Role of Ceveolae on Translocation of Glucose Transporter
Grant number：15590154 2003 - 2004
Ministry of Education, Culture, Sports, Science and Technology Grants-in-Aid for Scientific Research(基盤研究(C)) 基盤研究(C)
Takeo AOKI, Toshiyuki MATSUZAKI, Haruo HAGIWARA, Kuniaki TAKATA, Takeshi SUZUKI
Authorship：Collaborating Investigator(s) (not designated on Grant-in-Aid) Grant type：Competitive
Grant amount：\3600000 （ Direct Cost: \3600000 ）
I made GFP fusion protein to have full length of wild type Caveolin-1(human) which was the protein which constituted caveolae toward and transfected into a 3T3-L1 cell and I got selected this by antibiotics stably expressed cells. I was able to get GFP fusion proteins stably expressed cells to have N terminal portion deleted type of this protein. In addition, N terminal deletion type of Caveolin-3 and Caveolin-1 DNA in two places of amino acids mutation of apart of scaffolding domain of Caveolin-1.About a glucose transporter, I compared reaction for insulin stimulation after having guided fat cell differentiation with the 3T3-L1 cell which I transfected a GLUT4-GFP fusion type. As the factor which restrained the formation of caveolae, I transiently tranfected a N terminal portion deleted type (DGV) of Caveolin-3. As the factor which restrained the clathrin formation, I tranfected a C terminal part of AP180 DNA. I stimulated these two cells by insulin. As a result, a translocation of GLUT4 to a cell membrane was restrained by both protein expressions. This got a conclusion that both existence of clathrin coated vesicles and caveolae was restrained in a translocation to a cell membrane of a glucose transporter.I examined a translocation and recycling of aquaporin-2 to compare it with a translocation of GLUT4. I understood that a translocation of aquaporin-2 was obstructed by functional restraint of raft caused by a defect of glycosphingolipid. Furthermore, after recycling, aquaporin retrieved endosomes with Caveolin-1. I understood what was obstructed return to this cell when I transfected caveolin-1 which had variation into two places of amino acids of a part of scaffolding domain which restrained the formation of caveolae.
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輸送体膜タンパク質の細胞内局在移送の全行程を生きている細胞でみる
Grant number：14037209 2002
文部科学省 科学研究費補助金(特定領域研究) 特定領域研究
鈴木 健史
Authorship：Principal investigator Grant type：Competitive
Grant amount：\2300000 （ Direct Cost: \2300000 ）
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生体内コンパートメント間での水移送における水チャネルの役割の細胞構造的研究
Grant number：13137201 2001 - 2004
文部科学省 科学研究費補助金(特定領域研究(B), 特定領域研究) 特定領域研究(B), 特定領域研究
高田 邦昭, 鈴木 健史, 青木 武生, 萩原 治夫, 松崎 利行
Authorship：Collaborating Investigator(s) (not designated on Grant-in-Aid) Grant type：Competitive
Grant amount：\35300000 （ Direct Cost: \35300000 ）
アクアポリン水チャネル蛋白の中で、バソプレシン刺激により細胞膜と細胞内とを往き来して細胞膜の水輸送活性の調節をおこなっているアクアポリン2の細胞内局在と動態についてアクアポリン2を発現させたMDCK培養上皮細胞を使って解析をおこなった。オルガネラマーカーとの二重染色により、細胞内アクアポリン2はEEA1陽性の初期エンドソームや、Rab11陽性の頂部リサイクリングエンドソームと一部共局在することが判明した。上皮様シートを形成した細胞では、フォルスコリン刺激により細胞のサイクリックAMPレベルをあげると、ほとんどの細胞内アクアポリン2は頂部細胞膜へと移行した。このあとフォルスコリンを除き、シンクロナイズした細胞内への取り込みと細胞内での移行過程を観察した。アクアポリン2はまず初期エンドソームへ入り、ついでRab11陽性の頂部リサイクリングエンドソームへと戻った。頂部細胞膜へ移行するには、Rab11陽性のコンパートメントへ戻ることが必要であり、EEA1陽性コンパートメントはフォルスコリン非感受性であった。サイトカラシンDやラトランクリンB投与すると、細胞内のアクアポリン2は細胞膜をへてすみやかにEEA1陽性のコンパートメントへ移行し、ここに蓄積した。以上の結果から、アクチンは頂部細胞膜に移行したアクアポリン2を膜に保持するのに必要であるとともに、刺激により細胞膜へ移行可能な貯蔵部位への、初期エンドソームからのトラフィッキングにも重要な役割をはたしているのが判明した。
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上皮細胞における輸送体膜タンパク質の極性局在機構の分子形態学的解析
Grant number：13770002 2001 - 2002
文部科学省 科学研究費補助金(奨励研究(A), 若手研究(B)) 奨励研究(A), 若手研究(B)
鈴木 健史
Authorship：Principal investigator Grant type：Competitive
Grant amount：\2100000 （ Direct Cost: \2100000 ）
頂部細胞膜タンパク質のターゲティングに脂質ラフトが関与していることが近年多く報告されている.そこで,ナトリウムイオン依存型糖輸送体SGLT1の選択的な頂部細胞膜への移送や頂部細胞膜での局在に脂質ラフトが関与している可能性について検討した.このため,生きた細胞内でのSGLT1の動きを観察するために,GFP融合SGLT1をMDCK細胞に導入し安定して発現する株を作製した.この細胞では,コンフルエントの状態になるとSGLT1-GFPは頂部細胞膜のみに局在する.この細胞株を使い,脂質ラフトの主要成分であるコレステロールを除去することにより,SGLT1の頂部細胞膜局在がどのような影響を受けるかを解析した.メチル-β-シクロデキストリン(MCD)処理によって細胞に含まれているコレステロールの急激な除去を行うと,短時間で基底側壁部細胞膜上にもGFPのシグナルが出現し始め細胞膜全周に局在するようになった.この過程を,生きている細胞でタイムラプス観察した結果,MCD処理の影響は15〜30分後から始まり,2時間後には全ての細胞で細胞膜全周に局在するようになった.また,経時的にサンプリングした細胞を蛍光抗体法で解析した結果,SGLT1-GFPが全膜局在する過程におけるタイトジャンクションの染色パターンに変化は見られなかった.このSGLT1-GFPの細胞内局在におよぼすMCDの影響は,培地にコレステロールを添加することによって相殺された.以上の結果は,SGLT1の頂部細胞膜局在にコレステロールラフトが重要な役割を果たしていることを示唆している.
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Distribution sugar transporter isoforms and their regulation in the kidney
Grant number：11671535 1999 - 2002
Ministry of Education, Culture, Sports, Science and Technology Grants-in-Aid for Scientific Research(基盤研究(C)) 基盤研究(C)
Keiko FUJIKURA, 鈴木 健史, Kuniaki TAKATA
Authorship：Collaborating Investigator(s) (not designated on Grant-in-Aid) Grant type：Competitive
Grant amount：\3100000 （ Direct Cost: \3100000 ）
Various transporters, channels, pumps play important roles in the transfer of substances in the kidney. Precise localization and function of these molecules in the kidney are yet to be determined. In this project, we analyzed the localization of sugar transporters and organic cation transporters in the rat kidney. Fructose transporter GLUT5 was concentrated in the apical plasma membrane of S3 segment of proximal tubules in the outer stripe of the outer medulla. Insulin-recruitable sugar transporter GLUT4 was expressed in the cells of Henle's loop. It was localized intracellular compartments especially in the Golgi area including trans-Golgi network. In addition, it was localized in the endosomes. The dynamics of GLUT4 in the cell was analyzed in 3T3-L1 cells expressing green fluorescent protein (GFP)-tagged GLUT4 by the time-lapse microscopy of living cells. GLUT4-GFP was concentrated in the perinuclear region. Branching and fusion of GFP-GLUT4 were seen in this Golgi area. In the GFP-GLUT4 vesicles scattered in the cytoplasm, three types of movement were observed : One is immobile and remained in the same position. The second type of vesicles moved rapidly along the straight line for a long distance. The third type reciprocally moved, rapidly changing the direction of the motion. Organic cation transporters rOCTl and rOCT2 are expressed in the kidney, and their localization was visualized immunfluorescent technique. rOCTl was localized in the convoluted proximal tubules in the cortex, whereas rOCT2 was straight proximal tubules in the outer stripe of the outer medulla. Both transporters were localized along the basolateral plasma membrane, suggesting their role in the transfer of organic cations in the proximal tubules.
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Grant number：11877001 1999 - 2001
文部科学省 科学研究費補助金(萌芽的研究) 萌芽的研究
高田 邦昭, 鈴木 健史
Authorship：Collaborating Investigator(s) (not designated on Grant-in-Aid) Grant type：Competitive
Grant amount：\2100000 （ Direct Cost: \2100000 ）
アクアポリンは生体膜の水チャネルで、水、グリセロール、尿素などの低分子の生体膜通過に関与する膜蛋白質である。原核生物から動物、植物にまで普遍的に分布し、多種のアイソフォームが体の様々な部位に様々な程度に発現しているが、その生物学的な役割については不明の点が多い。アクアポリン3(AQP3)は尿に接する腎盤、尿管、膀胱、尿道などの尿路上皮、体表を覆う表皮をはじめとする重層扁平上皮、上部と下部の消化管上皮、鼻腔から気管支に至る気道上皮などの細胞に発現していた。その局在部位を、凍結準超薄切片/CCDカメラ蛍光顕微鏡を用いて詳細に観察した。その結果、重層上皮では、基底部から中間層の細胞の細胞膜、単層上皮では基底側壁部細胞膜に局在した。ナノゴールドプローブを用いたプレエンベッディング免疫電顕法により、これらの細胞の部位細胞膜に局在するのが確認された。外気や尿に接する表層の細胞や頂部細胞膜にはAQP3の分布は認められなかった。MDCK細胞では、高浸透圧処理により、AQP3の発現はまずメッセンジャーレベルで、ついで蛋白レベルで増加した。この場合もナノゴールド免疫電顕法により、基底側壁部細胞膜にAQP3は局在した。
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ナトリウムイオン依存型糖輸送体SGLT1の頂部細胞膜局在機構の分子形態学の解析
Grant number：11770002 1999 - 2000
文部科学省 科学研究費補助金(奨励研究(A)) 奨励研究(A)
鈴木 健史
Authorship：Principal investigator Grant type：Competitive
Grant amount：\2200000 （ Direct Cost: \2200000 ）
本研究は,糖輸送体の高度に分化した高等動物組織細胞内での局在分布機構,特に間接的にエネルギーを消費し能動的に糖を細胞に取り込むナトリウムイオン依存型糖輸送体SGLT1の頂部細胞膜への移送経路の解明を目的に行った.SGLT1遺伝子の発現を自在にコントロールする事によってその頂部細胞膜局在メカニズムを移送・局在機構を分離して解析するために,培地に添加するドキシサイクリンの濃度に応じてSGLT1遺伝子発現のオン・オフと発現量を調節できるシステムをMDCK細胞に組み込んだTetOff-SGLT1遺伝子発現システムを構築した.さらにこのシステムを利用し,ドキシサイクリンを培地から除去しSGLT1を発現誘導した後に培地にテトラサイクリンを再添加し遺伝子発現を停止させチェイス培養するパルス発現-チェイス培養システムを構築した.様々な時間チェイス培養した後にMDCK細胞を固定し,発現産物の細胞内局在を,凍結準超薄切片蛍光抗体染色法,共焦点レーザー顕微鏡法,蛍光顕微鏡法,免疫電子顕微鏡法などを組み合わせ解析する事によって,SGLT1が細胞内で合成された後に直接頂部細胞膜に移送されていることが明らかとなった.現在このシステムを用いて,SGLT1の頂部細胞膜への直接的な移送システムが細胞極性形成過程の何処で構築されるのかを解析中である.
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Analysis of cell polarity formation by gene transfection in organs in situ
Grant number：10470003 1998 - 2001
Ministry of Education, Culture, Sports, Science and Technology Grants-in-Aid for Scientific Research(基盤研究(B)) 基盤研究(B)
Kuniaki TAKATA, 鈴木 雅一, Takeshi SUZUKI
Authorship：Collaborating Investigator(s) (not designated on Grant-in-Aid) Grant type：Competitive
Grant amount：\12700000 （ Direct Cost: \12700000 ）
In vivo electroporation and particle gun methods were used to introduce exogenous genes into tissue cells in situ in living animals. Constructs of green fluorescent proteins were tested to optimize the conditions of gene transfection. Glucose transporters (GLUT1, GLUT3, GLUT4, GLUT5, and SGLT1) were introduced into cells of liver and pancreas of living rats. The expressed gene products were visualized by immunofluorescence staining of tissue sections. By optimizing the conditions of transfection, glucose transporters were successfully expressed in highly differentiated cells such as hepatocytes and pancreatic acinar cells forming tissues in situ. GLUT1 was localized to basolateral membrane domains in both hepatocytes and pancreatic acinar cells. GLUT3 and GLUT5 were restricted to apical membrane domain in pancreatic acinar cells. They were found in both the apical and basolateral domains in hepatocytes. These observations suggest differential targeting mechanism in hepatocytes and acinar cells : i.e., apical targeting is carried out by direct pathway in acinar cells, whereas it is mediated by the indirect transcytotic pathway in hepatocytes. GLUT4 was retained in the cytoplasmic compartments in both cell types. Immuno-electron microscopy revealed that GLUT4 resides in the membranes of zymogne secretary granules in acinar cells, showing that GLUT4 is sorted to the regulated secretion pathway in these cells. In addition, in vivo electroporation and particle gun methods are simple and versatile methods to study the localization of exogenous gene products in cells and tissues in situ.
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Development of immunohistochemistry using confocal microscopy and microinjection method
Grant number：09557002 1997 - 2000
Ministry of Education, Culture, Sports, Science and Technology Grants-in-Aid for Scientific Research(基盤研究(B)) 基盤研究(B)
Kuniaki TAKATA, 鈴木 雅一, Takeshi SUZUKI, Masakazu SUZUKI
Authorship：Collaborating Investigator(s) (not designated on Grant-in-Aid) Grant type：Competitive
Grant amount：\11800000 （ Direct Cost: \11800000 ）
In the confocal microscopic examination of biological specimens, localization of nuclei gives important information. We screened fluorescent dyes, originally developed for nucleic acids in gel, for the use in multi-fluorescence microscopy. Among dyes tested SYBR GreenI, SYTOX Green, Pico Green are best suited for green fluorescence, YO-PRO-3 for red fluorescence, and TO-PRO-3 for far red fluorescence. Use of these dyes for nuclear-counter staining in salivary glands proved to give superior confocal images, suitable for the reconstruction of the glands. Localization of aquaporin 5 was clear demonstrated in these complex acinus and duct systems. Nuclear fluorescent counterstaining is also useful in determining the localization of channels and transporters in cultured epithelial cells. For the introduction of exogenous genes into cells, we used microinjection and lipofection methods We transiently expressed GLUT4 glucose transporter in cultured cells, and its immunolocalization and translocation to the plasma membrane was monitored with conventional and confocal fluorescence microscopes. We also used green-fluorescen-protein-tagged GLUT4 and its localization was analyzed. Introduction of exogenous genes, followed by three-dimonsional confocal image analyses proved to be a very convenient and powerful tool in the identification of gene product expressed in the cells.
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Grant number：09877002 1997 - 1998
文部科学省 科学研究費補助金(萌芽的研究) 萌芽的研究
高田 邦昭, 鈴木 雅一, 鈴木 健史
Authorship：Collaborating Investigator(s) (not designated on Grant-in-Aid) Grant type：Competitive
Grant amount：\2300000 （ Direct Cost: \2300000 ）
MDCK細胞に糖輸送体GLUT1,GLUT5,SGLT1の遺伝子を,リポフェクション法により導入した.これらを物質透過性のある膜のトランスウエル上で培養し上皮様の細胞層を得た.それぞれの糖輸送体に特異的な抗体を用いて蛍光抗体染色し,導入した遺伝子の発現と局在を検討した.同時にタイトジャンクション蛋白のオクルディンを特異抗体で,核をDNA特異的なTO-PRO3で染色し,3チャネル共焦点レーザー顕微鏡で観察した.コンフルエントになった細胞シートでは,GLUT1は基本的にはMDCK細胞の基底側壁部細胞膜に局在した.一方GLUT5とSGLT1はMDCK細胞の頂部細胞膜に局在した.GLUT1,GLUT5,SGLT1の中のどれか二つを用いたコトランスフェクションと蛍光二重染色によっても同様の結果が得られた.GLUT1の発現を時間をおって観察した結果,GLUT1はまず細胞内から細胞膜へ輸送され全周にわたって分布した.やがてGLUT1は基底側壁部細胞膜に多くみられるようになり,最終的には基底側壁部細胞膜に限局して局在した.GLUT5も最初はGLUT1と同様に細胞膜全周にわたって存在したが,頂部細胞膜により多く局在するようになった.一方SGLT1は最初は主に細胞内部に存在し,やがて培養液に接する細胞の頂部に陽性となった.今回の条件ではSGLT1はGLUT5とは異なり,細胞膜全周への局在はほとんど見られなかった.現在,頂部微絨毛を一ヵ所に集める作用のあるジャスプラキンを用いて頂部細胞膜を微絨毛とその他のミクロドメインに分けた場合の分布を,高解像の共焦点レーザー顕微鏡と電顕を使い検討中である.
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上皮細胞極性形成過程における糖輸送体の選択的細胞膜局在機構の分子形態学的解析
Grant number：09770003 1997 - 1998
文部科学省 科学研究費補助金(奨励研究(A)) 奨励研究(A)
鈴木 健史
Authorship：Principal investigator Grant type：Competitive
Grant amount：\1900000 （ Direct Cost: \1900000 ）
本研究の目的は,糖輸送体分子の細胞内局在機構を解明することである.そのため,糖輸送体遺伝子を遺伝子工学的手法によって改変し極性を持つ上皮培養細胞(MDCK細胞)や生体組織細胞に導入発現させ,その挙動をレーザー共焦点顕微鏡法等で観察した.実験には腎臓や小腸上皮細胞で頂部細胞膜に局在する糖輸送体SGLT1を用いた.野生型のSGLT1遺伝子を導入したMDCK細胞を蛍光抗体染色しレーザー共焦点顕微鏡で観察したところ,頂部細胞膜に限局していた.しかし,N末部の膜外突出部位にデリーションやアミノ酸置換などの変異を導入したcDNAクローンを上皮培養細胞に導入したところ頂部細胞膜局在がみだされ,頂部および基底側壁部の全細胞膜に局在するような,あるいは細胞膜には組み込まれず細胞質コンパートメントに局在を示すようになった.頂部細胞膜局在を乱す変異は第一膜貫通領域の直前にある9残基のアミノ酸配列内に限られていた.以上の結果は,SGLT1のN末膜外突出部位にある短い配列が頂部細胞膜局在の調節システムに深く関与していることを示唆する.こうした現象が培養細胞だけでなく実際の生体組織細胞でも起こりうるのかを確認するために,生体組織細胞へ直接cDNAクローンを導入する方法として,生体エレクトロポレーション法を確立した.また,組織に火傷を伴わない方法として,現在ジーンガンによるパーティクルデリバリーシステムの導入を試みている.
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糖の吸収供給機能を制御する糖輸送体の細胞膜ドメイン局在分布機構の分子形態学的解析
Grant number：07770004 1995
文部科学省 科学研究費補助金(奨励研究(A)) 奨励研究(A)
鈴木 健史
Authorship：Principal investigator Grant type：Competitive
Grant amount：\1100000 （ Direct Cost: \1100000 ）
本研究の目的は,糖輸送体分子の細胞内局在分布機構を解明することにある.このため,糖輸送体遺伝子を遺伝子工学的手法によって改変し,それを極性を持つ細胞に導入発現させ,発現した産物の挙動を高解像度蛍光抗体染色法,金コロイド標識法,レーザーコンフォーカル顕微鏡法等を組み合わせて観察した.その結果、正常なSGLT1は頂部膜ドメインに局在発現し,SMITは基底側壁部に局在発現した.SGLT1のN末部の30アミノ酸を除いた欠失遺伝子の産物は細胞膜全域に発現した.さらに,そのアミノ酸の代わりにSMITのN末部の10アミノ酸を加えたキメラ遺伝子産物は基底側壁部に局在発現した.この結果は,これらの糖輸送体の細胞内局在機構を規定するドメインがN末部の短いアミノ酸配列であることを示している.
Teaching Experience
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実験生物学（面接授業）
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解剖学
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生物学実験（自然科学実験）
Institution：札幌医科大学
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基礎生物学
Institution：札幌医科大学
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生体防御学（免疫学）
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免疫学１
Institution：札幌医科大学
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生物学
Institution：札幌医科大学
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組織学
Institution：群馬大学
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組織実習
Institution：群馬大学
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神経解剖学
Institution：群馬大学
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脳実習
Institution：群馬大学
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解剖生理学