Education 【 display / non-display 】

Education 【 display / non-display 】

• 　

  -

  2014

  Sapporo Medical University   Graduate School of Medicine  

• 　

  -

  2011

  Sapporo Medical University   Graduate School of Medicine  

• 　

  -

  2009

  Tenshi College   School of Nursing and Nutrition  

• 　

  -

  1997

  Hokkaido University of Education   Faculty of Education  

Degree 【 display / non-display 】

Degree 【 display / non-display 】

• 学士 (教育学)

• 学士 (栄養学)

• 博士 (医学)

• 修士 (医科学)

Research Experience 【 display / non-display 】

Research Experience 【 display / non-display 】

• 2014

  　

  　

  Sapporo Medical University   School of Medicine Medical Sciences  

• 1997

  　

  　

  札幌市公立小学校 教員  

Professional Memberships 【 display / non-display 】

Professional Memberships 【 display / non-display 】

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  JAPANESE SOCIETY FOR BACTERIOLOGY

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  THE JAPANESE ASSOCIATION FOR INFECTIOUS DISEASES

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  JAPANESE SOCIETY OF PUBLIC HEALTH

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  THE JAPANESE SOCIETY FOR HYGIENE

• 　

  　

  　

  日本臨床微生物学会

Affiliation 【 display / non-display 】

Affiliation 【 display / non-display 】

• Sapporo Medical University   医学部医学科 基礎医学部門 衛生学講座  

Research Interests 【 display / non-display 】

Research Interests 【 display / non-display 】

• 分子疫学

• 公衆栄養学

• 予防医学

• 微生物学

• 公衆衛生学

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Papers 【 display / non-display 】

Papers 【 display / non-display 】

• Serotype distribution and antimicrobial resistance of Streptococcus pneumoniae isolated from children in Japan, 2023.

  Mitsuyo Kawaguchiya, Noriko Urushibara, Meiji Soe Aung, Nobuhide Ohashi, Sho Tsutida, Kayo Kurashita, Masahiko Ito, Nobumichi Kobayashi

  New microbes and new infections   62   101513 - 101513  2024.12  [Refereed]  [International journal]

  Authorship：   Lead author  , Corresponding author

  　View Summary

  BACKGROUND: The prevalence of serotypes and antimicrobial resistance of Streptococcus pneumoniae was characterized among children thirteen years after the licensure of the pneumococcal conjugate vaccine (PCV) in Japan. METHODS: A total of 353 pneumococcal isolates were collected from Japanese children between March and July 2023. All the isolates were serotyped using genetic methods and tested for susceptibility to 14 antimicrobial agents. RESULTS: Among the 353 isolates, the prevalence rates of non-PCV13 and non-PCV20 serotypes were 96.9 % and 77.9 %, respectively, including the dominant non-PCV13/PCV20 serotypes 23A (16.1 %), 35B (15.3 %), 15A (10.5 %), 15C (9.3 %), and 34 (9.1 %), which accounted for 60.3 % of all isolates. The high non-susceptibility rates were observed for macrolides (erythromycin, azithromycin, and clarithromycin; ≥81.9 %) and tetracycline (80.7 %). Penicillin non-susceptibility and multidrug resistance (MDR) were detected in 49.9 % (6.8 % resistant and 43.1 % intermediate) and 68.3 % of the isolates, respectively. The three most common non-PCV13/PCV20 serotypes 15A, 23A, and 35B exhibited high prevalence rates of penicillin non-susceptibility (≥89.5 %) and MDR (≥81.5 %). Extensive drug resistance was observed in 10.5 % of all isolates belonging to six different serotypes (12F, 23A, 11A, 15A, 35B, and 15B) and in the non-encapsulated strains of S. pneumoniae. CONCLUSIONS: Our study revealed a higher prevalence of non-susceptibility to penicillin with MDR in the three most common non-PCV13/PCV20 serotypes 15A, 23A, and 35B, in Japan, suggesting their persistence in the PCV13 era.

  DOI PubMed

• Prevalence and antimicrobial resistance of three clones (ST1223, ST2198, ST2250) of Staphylococcus argenteus clinical isolates in northern Japan.

  Meiji Soe Aung, Noriko Urushibara, Mitsuyo Kawaguchiya, Nobuhide Ohashi, Mina Hirose, Kenji Kudo, Masahiko Ito, Nobumichi Kobayashi

  Journal of global antimicrobial resistance    2024.11  [Refereed]  [International journal]

  　View Summary

  OBJECTIVES: Staphylococcus argenteus, a newly described species of coagulase-positive Staphylococcus, was first identified in clinical isolates in northern Japan in 2019. The aim of the present study was to clarify the trends in the epidemiological features and antimicrobial resistance traits of S. argenteus in the recent years. METHODS: S. argenteus isolates from various clinical specimens were screened using MALDI-TOF and genotyped using the MLST scheme. Antimicrobial susceptibility was determined using broth microdilution test and resistance genes were detected by multiplex/uniplex PCR. Nucleotide sequences of hemolysin genes (hla, hlb, hld, and hlg) were determined and analyzed in some isolates. RESULTS: From August 2020 to December 2023, 210 isolates were identified as S. argenteus. The ratio of S. argenteus to S. aureus clinical isolates was 0.0045, showing a slightly decreasing trend compared to what was previously reported (2019.8-2020.7). Three genotypes, ST1223-coa-XV, ST2198-coa-XIV, and ST2250-coa-XId were identified, with ST2250 being dominant (49%) and exhibiting an increasing trend. S. argenteus isolates were almost susceptible to antimicrobials examined, while ST2198 isolates showed higher resistance rates to ampicillin, macrolides, and aminoglycosides than other clones, harboring blaZ, msrA, and aac(6')-Ie-aph(2")-Ia. Only eight isolates (4-ST2250, 3-ST1223, and 1-ST2198) did not show hemolysis on blood agar plates. These isolates had intact hemolysin genes and promoter regions; however, a novel deletion mutation in hlg-component A was detected in some hemolytic and non-hemolytic isolates. CONCLUSIONS: The present study revealed the epidemiological trend of S. argenteus clones in northern Japan, along with the occurrence of non-hemolytic isolates with intact hemolysin genes.

  DOI PubMed

• Genetic characterization of methicillin-resistant / susceptible Staphylococcus aureus (MRSA/MSSA) and Staphylococcus argenteus clinical isolates in Bangladesh: Dominance of ST6-MRSA-IV/t304 and detection of cfr/fexA in ST8-MSSA/t008

  Sangjukta Roy, Meiji Soe Aung, Shyamal Kumar Paul, Syeda Anjuman Nasreen, Nazia Haque, Rummana Mazid, Md Shahed Khan, Tridip Kanti Barman, Parvez Arafa, Fardousi Akter Sathi, Sultana Shabnam Nila, Afsana Jahan, Noriko Urushibara, Mitsuyo Kawaguchiya, Nobuhide Ohashi, Nobumichi Kobayashi

  IJID Regions   10   132 - 139  2024.03  [Refereed]

  　View Summary

  Objectives: Coagulase-positive staphylococcus (CoPS), represented by Staphylococcus aureus, is a major cause of infections in humans. This study aimed to investigate molecular epidemiological characteristics, antimicrobial resistance, and their trends of CoPS in Bangladesh. Methods: Clinical isolates of CoPS were collected from two medical institutions in Bangladesh for a 2-year period and analyzed for their species, genotypes, virulence factors, antimicrobial susceptibility, and resistance determinants. Results: 172 CoPS isolates collected were identified as S. aureus or S. argenteus (170 and two, respectively). Methicillin-resistant S. aureus (MRSA) accounted for 36% (n = 61), having Staphylococcal cassette chromosome mec (SCCmec)-IV (82%) or V (18%). Panton-Valentine leukocidin (PVL) genes were detected at higher rate in methicillin-susceptible S. aureus (MSSA) (62%) than MRSA (26%). MRSA comprised 11 STs, including a dominant type ST6 (46%) associated with mostly SCCmec-IVa/spa-t304, and one isolate had genetic features of the USA300 clone (ST8/SCCmec-IVa/coa-IIIa/spa-t008/ACME-I/ΦSa2USA). STs of CC1, CC88, and CC398 were common in MSSA, with CC88 showing the highest PVL-positive rate. One MSSA isolate (ST8/spa-t008) harbored fexA and cfr showing susceptibility to linezolid. S. argenteus was methicillin-susceptible and belonged to ST2250/coa-XId. Conclusions: Genetic characteristics of current MRSA/MSSA in Bangladesh were revealed, with first identification of S. argenteus at low prevalence.

  DOI

• Molecular Epidemiological Characterization of Methicillin-Resistant Staphylococcus aureus from Bloodstream Infections in Northern Japan: Increasing Trend of CC1 and Identification of ST8-SCCmec IVa USA300-Like Isolate Lacking Arginine Catabolic Mobile Element.

  Meiji Soe Aung, Noriko Urushibara, Mitsuyo Kawaguchiya, Nobuhide Ohashi, Mina Hirose, Yuuki Kimura, Kenji Kudo, Masahiko Ito, Nobumichi Kobayashi

  Microbial drug resistance (Larchmont, N.Y.)    2023.12  [Refereed]  [International journal]

  　View Summary

  Methicillin-resistant Staphylococcus aureus (MRSA) is a major infectious disease pathogen, and its molecular epidemiological profile has been changing. In this study, a total of 279 MRSA isolates were collected from patients with bloodstream infection (BSI) in Hokkaido, northern main island of Japan, for a 2-year period from August 2019 to July 2021. CC5 (ST5/ST764)-MRSA-IIa (SCCmec-IIa) (47%, n = 132) and CC1 (ST1/ST2725/ST2764)-MRSA-IVa (42%, n = 116) were found to be major lineages, with CC8-MRSA-IVa being lower prevalence (5%, n = 13). CC1-MRSA-IVa showed a relatively increased proportion compared with our previous study (22%, 2017-2019). Seven isolates with SCCmec IVa (2.5%) were positive for Panton-Valentine leukocidin genes on ΦSa2usa and belonged to ST8/spa-t008/agr-I/coa-IIIa, showing genetic features of the USA300 clone. Among these isolates, six isolates harbored arginine catabolic mobile element (ACME) type I typical to the USA300 clone, while it was not detected in an isolate (strain R3-8). Whole genomic analysis of strain R3-8 revealed that its chromosome was highly similar to the USA300 strain TCH1516, but lacked ACME, carrying a plasmid genetically close to that of USA300 strains. The present study revealed increasing trend of CC1-MRSA-IV and occurrence of a novel variant of the USA300 clone among MRSA from BSI in northern Japan.

  DOI PubMed

• Prevalence, clonal diversity, and antimicrobial resistance of hypervirulent Klebsiella pneumoniae and Klebsiella variicola clinical isolates in northern Japan

  Norifumi Matsuda, Meiji Soe Aung, Noriko Urushibara, Mitsuyo Kawaguchiya, Nobuhide Ohashi, Kenji Taniguchi, Kenji Kudo, Masahiko Ito, Nobumichi Kobayashi

  Journal of Global Antimicrobial Resistance   35   11 - 18  2023.12  [Refereed]  [International journal]

  　View Summary

  Objectives: Hypervirulent Klebsiella pneumoniae (hvKp) and Klebsiella variicola (hvKv) cause hospital/community-acquired infections, often associated with antimicrobial resistance (AMR). This study aimed to investigate the molecular epidemiology of hvKp and hvKv in northern Japan. Methods: A total of 500 K. pneumoniae and 421 K. variicola clinical isolates collected from August to December 2021 were studied. Prevalence of virulence factor-encoding genes, wzi sequence and associated K/KL type, sequence type (ST), and beta-lactamases and their types were characterized. Results: Any virulence gene (rmpA, rmpA2, peg-344, iucA, iutA, and iroB) and/or magA was detected in 25% (n = 125) of K. pneumoniae and 1% (n = 5) of K. variicola. Among these hvKp/hvKv, 22 wzi types (18 and 4 types, respectively) and 24 STs (20 and 4 STs, respectively) were identified. Sequence types of hvKp were classified into some clonal groups (CGs), among which CG35, including six STs, was the most common (n = 59; 47%), followed by CG23, and CG65. ST268 (CG35) associated with wzi95-K20 or wzi720 was the dominant lineage (n = 43, 34%), while K1:ST23/ST249 and K2:ST65/ST86 accounted for 26% and 13% of hvKp, respectively. Extended-spectrum beta-lactamase (ESBL) genes (blaCTX-M-2, blaCTX-M-3, blaCTX-M-15, and blaCTX-M-27) were detected in only ST23 and CG35 (ST268 and ST412) hvKp. No isolate was resistant to carbapenems, without detection of the ESBL gene in K. variicola. Phylogenetically, wzi was differentiated into two main clusters of K. pneumoniae and K. variicola. A major clonal group CG347 was identified in K. variicola. Conclusion: Clonal structures were revealed for hvKp and hvKv clinical isolates with their AMR status in northern Japan.

  DOI PubMed

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Misc 【 display / non-display 】

Misc 【 display / non-display 】

• 2023年に小児から分離された肺炎球菌の血清型分布の動向

  川口谷 充代, 漆原 範子, Meiji Soe Aung, 大橋 伸英, 木村 優希, 堀野 裕香, 伊藤政彦, 小林宣道

  日本細菌学雑誌 79 (2) ( 日本細菌学会 )  79 ( 2 ) 98 - 98  2024.08

• Molecular epidemiological characterization of MRSA from bloodstream infections in Hokkaido(タイトル和訳中)

  Aung Meiji Soe, 漆原 範子, 川口谷 充代, 大橋 伸英, 荒木 蕗羽, 松原 加奈, 伊藤 政彦, 小林 宣道

  日本細菌学雑誌 ( 日本細菌学会 )  79 ( 2 ) 73 - 73  2024.06

• Characteristics of Staphylococcaceae in Retail Meat Products in Hokkaido: A One Health Perspective(タイトル和訳中)

  漆原 範子, アウン・メイジソウ, 川口谷 充代, 大橋 伸英, 小林 宣道

  日本細菌学雑誌 ( 日本細菌学会 )  79 ( 2 ) 100 - 100  2024.06

• 北海道における血流感染症由来MRSAの分子疫学的解析

  小林宣道, アウン メイジソウ, 漆原範子, 川口谷充代, 大橋伸英, 荒木蕗羽, 松原加奈, 伊藤政彦

  日本衛生学雑誌(Web)   79 ( Supplement )  2024

  J-GLOBAL

• 肺炎球菌結合型ワクチン導入10年後における肺炎球菌の血清型分布と薬剤感受性

  川口谷充代, 漆原範子, アウン メイジソウ, 大橋伸英, 高松里佳, 土田翔, 伊藤政彦, 小林宣道

  日本衛生学雑誌(Web)   79 ( Supplement )  2024

  J-GLOBAL

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Awards 【 display / non-display 】

Awards 【 display / non-display 】

• 平成29年度 北海道公衆衛生協会賞：北海道における非侵襲性感染症由来肺炎球菌の血清型と薬剤耐性に関する分子疫学的研究

  2017  

  Winner： 川口谷 充代

Research Projects 【 display / non-display 】

Research Projects 【 display / non-display 】

• Molecular epidemiology and comprehensive exploration of novel virulence factors and their action mechanisms of hypervirulent Staphylococcus in Asia

  Fund for the Promotion of Joint International Research (International Collaborative Research)

  Project Year :

  2023.09

  -

  2027.03

   

  小林 宣道, 漆原 範子, 川口谷 充代, アウン メイジソウ, 大橋 伸英

• ワクチン普及後に拡大した新興の多剤耐性肺炎球菌感染症制御のための分子疫学的研究

  基盤研究(C)

  Project Year :

  2022.04

  -

  2025.03

   

  川口谷 充代, 小林 宣道

• Molecular epidemiological study of novel species and emerging drug-resistant staphylococcus in Japan and Asia, and basic construction of their infection control

  Grant-in-Aid for Scientific Research (B)

  Project Year :

  2020.04

  -

  2024.03

   

  小林 宣道, アウン メイジソウ, 川口谷 充代, 漆原 範子

  　View Summary

  本研究の目的は、薬剤耐性および臨床検査の面で近年注目されている新規ブドウ球菌種S.argenteus、市中感染型MRSA（CA-MRSA）、抗MRSA薬耐性菌等について、その疫学的状況を明らかにし感染対策の方策を探ることである。今年度は北海道において分離されたS.argenteus 82株、血液由来CA-MRSA 277株を対象として、分子疫学的解析を行った。S.argenteusは3つの遺伝子型ST1223、ST2198、ST2250に分類され、ST2250が最も多かった。STにより病原因子、特にエンテロトキシン（様）遺伝子と、薬剤耐性に違いが認められた。ST2198株は、他の2つのSTに比べ薬剤耐性遺伝子の検出率および一部薬剤への耐性率が有意に高かった。Panton-Valentine leukocidin(PVL)陽性菌、SCCmecIVcを有するメチシリン耐性菌がST2250において1株ずつ同定された。血液由来CA-MRSAでは、SCCmecIIaを保有するST5/ST764/ST2389が最も多く、次いで多かったのはSCCmecIVa、IVlを有する株であった。米国で優勢なUSA300と同じ特徴（SCCmecIVa/ST8/PVL+/ACME+）の株が全体の5.1％を占めており、以前のデータと比較して増加の傾向が示唆された。一方、PVLを保有しないSCCmecIVa MRSAが64株（23％）検出され、それらは多様な遺伝子型に属していた。USA300クローンが典型的に有するspeG遺伝子が、ST764-SCCmecIIaのMRSA2株に存在することが判明し、新たな特徴を持つMRSAが出現していることが示唆された。

• 蛋白結合型ワクチン導入後に分離された 無莢膜型肺炎球菌の薬剤耐性と分子疫学的特徴

  Project Year :

  2019

  -

  2021

   

  川口谷充代

  Authorship： Principal investigator

• Molecular epidemiology of Staphylococcus aureus, MRSA having novel enterotoxins and virulence factors

  Grant-in-Aid for Scientific Research (C)

  Project Year :

  2018.04

  -

  2021.03

   

  Aung Meiji Soe

  　View Summary

  Prevalence of novel staphylococcal enterotoxin (SE)(-like) toxin genes in Staphylococcus aureus and its relative species S. argenteus, and their molecular epidemiological characteristics were analyzed. selw and selx were detected in more than 80% of S. aureus isolates examined, and showed diversity in their nucleotide sequences. S. aureus harbored sey and selz at low rate, without having sel26 and sel27. The sey gene was detected in all the ST2250 S. argenteus isolates, and more than half of this lineage harbored selz, sel26, and sel27. Prevalence of novel SE(-like) genes was revealed to be different depending on lineages of S. aureus and S. argenteus.

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Presentations 【 display / non-display 】

Presentations 【 display / non-display 】

• Serotype distribution and antimicrobial resistance of Streptococcus pneumoniae isolated from a university hospital in Japan.

  Kawaguchiya M., Urushibara N., Shinagawa M., Kobayashi N.

  The 48th Asia-Pacific Academic Consortium for Public Health Conference：Tokyo, Japan. 

  Event date：

  2016.09

  　

  　

• 7価および13価肺炎球菌結合型ワクチン導入による非侵襲性肺炎球菌臨床分離株の血清型の変化

  川口谷 充代  [Invited]

  北海道臨床微生物フォーラム 2015 

  Event date：

  2015

  　

  　

• Novel multiplex PCR in combination with mutagenic PCR-RFLP analysis to distinguish Streptococcus pneumoniae serotypes 6A, 6B, 6C, and 6D.

  Kawaguchiya M., Urushibara N., Kobayashi N.

  The 12th Japan-Korea International Symposium on Microbiology：Tokyo, Japan. 

  Presentation date： 2014.03

  Event date：

  2014.03

  　

  　

• Molecular epidemiological study of methicillin-resistant Staphylococcus aureus (MRSA) from community in northern Japan.

  Kobayashi N., Aung MS, Kawaguchiya M., Urushibara N., Sumi A., Quiñones D.

  Congreso “80 Aniversario del Instituto de Medicina Tropical, Pedro Kourí”, IX Congreso Cubano de Microbiología y Parasitología, VI Congreso Nacional de Medicina Tropical, VI Seminario Internacional sobre la Infección por el VIH y el sida en Cuba. 

  Presentation date： 2017.11

• Panton-Valentine leucocidin (PVL)/ Arginine catabolic mobile element (ACME)-positive methicillin-resistant Staphylococcus aureus (MRSA) in the community and the hospital in Japan.

  Mitsuyo Kawaguchiya  [Invited]

  The 17th International Symposium on Staphylococci and Staphylococcal infections (ISSSI): Seoul, Korea. 

  Presentation date： 2016.08

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Teaching Experience 【 display / non-display 】

Teaching Experience 【 display / non-display 】

• 衛生学  

  札幌医科大学  

• 基礎配属  

  札幌医科大学  

• 医学英語  

  札幌医科大学  

• 社会医学実習  

  札幌医科大学  

• 小学校全教科  

  札幌市公立小学校  

Academic Activities 【 display / non-display 】

Academic Activities 【 display / non-display 】

• Microorganisms 査読

  https://www.mdpi.com/journal/microorganisms  

• IJID Regions 査読

  https://www.sciencedirect.com/journal/ijid-regions  

• Pathogens 査読

  https://www.mdpi.com/journal/pathogens  

• BMC Infectious Diseases 査読

  https://bmcinfectdis.biomedcentral.com/  

• Frontiers 査読

  https://www.frontiersin.org/