KAWAGUCHIYA Mitsuyo

写真a

Affiliation

School of Medicine, Department of Hygiene

Job title

Lecturer

Homepage URL

https://nrid.nii.ac.jp/ja/nrid/1000070733062/

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Education 【 display / non-display 】

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    2014

    Sapporo Medical University   Graduate School of Medicine  

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    2011

    Sapporo Medical University   Graduate School of Medicine  

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    2009

    Tenshi College   School of Nursing and Nutrition  

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    1997

    Hokkaido University of Education   Faculty of Education  

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Degree 【 display / non-display 】

  • 学士 (教育学)

  • 学士 (栄養学)

  • 修士 (医科学)

  • 博士 (医学)

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Research Experience 【 display / non-display 】

  • 2014
    　
    　

    Sapporo Medical University   School of Medicine Medical Sciences  

  • 1997
    　
    　

    札幌市公立小学校 教員  

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Professional Memberships 【 display / non-display 】

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    JAPANESE SOCIETY FOR BACTERIOLOGY

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    THE JAPANESE ASSOCIATION FOR INFECTIOUS DISEASES

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    JAPANESE SOCIETY OF PUBLIC HEALTH

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    THE JAPANESE SOCIETY FOR HYGIENE

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    日本臨床微生物学会

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Affiliation 【 display / non-display 】

  • Sapporo Medical University   医学部医学科 基礎医学部門 衛生学講座  

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Research Interests 【 display / non-display 】

  • 分子疫学

  • 公衆栄養学

  • 予防医学

  • 微生物学

  • 公衆衛生学

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Papers 【 display / non-display 】

  • Molecular Epidemiological Characterization of Methicillin-Resistant Staphylococcus aureus from Bloodstream Infections in Northern Japan: Increasing Trend of CC1 and Identification of ST8-SCCmec IVa USA300-Like Isolate Lacking Arginine Catabolic Mobile Element.

    Meiji Soe Aung, Noriko Urushibara, Mitsuyo Kawaguchiya, Nobuhide Ohashi, Mina Hirose, Yuuki Kimura, Kenji Kudo, Masahiko Ito, Nobumichi Kobayashi

    Microbial drug resistance (Larchmont, N.Y.)    2023.12  [International journal]

    　View Summary

    Methicillin-resistant Staphylococcus aureus (MRSA) is a major infectious disease pathogen, and its molecular epidemiological profile has been changing. In this study, a total of 279 MRSA isolates were collected from patients with bloodstream infection (BSI) in Hokkaido, northern main island of Japan, for a 2-year period from August 2019 to July 2021. CC5 (ST5/ST764)-MRSA-IIa (SCCmec-IIa) (47%, n = 132) and CC1 (ST1/ST2725/ST2764)-MRSA-IVa (42%, n = 116) were found to be major lineages, with CC8-MRSA-IVa being lower prevalence (5%, n = 13). CC1-MRSA-IVa showed a relatively increased proportion compared with our previous study (22%, 2017-2019). Seven isolates with SCCmec IVa (2.5%) were positive for Panton-Valentine leukocidin genes on ΦSa2usa and belonged to ST8/spa-t008/agr-I/coa-IIIa, showing genetic features of the USA300 clone. Among these isolates, six isolates harbored arginine catabolic mobile element (ACME) type I typical to the USA300 clone, while it was not detected in an isolate (strain R3-8). Whole genomic analysis of strain R3-8 revealed that its chromosome was highly similar to the USA300 strain TCH1516, but lacked ACME, carrying a plasmid genetically close to that of USA300 strains. The present study revealed increasing trend of CC1-MRSA-IV and occurrence of a novel variant of the USA300 clone among MRSA from BSI in northern Japan.

    DOI PubMed

  • Prevalence, clonal diversity, and antimicrobial resistance of hypervirulent Klebsiella pneumoniae and Klebsiella variicola clinical isolates in northern Japan

    Norifumi Matsuda, Meiji Soe Aung, Noriko Urushibara, Mitsuyo Kawaguchiya, Nobuhide Ohashi, Kenji Taniguchi, Kenji Kudo, Masahiko Ito, Nobumichi Kobayashi

    Journal of Global Antimicrobial Resistance   35   11 - 18  2023.12

    　View Summary

    Objectives: Hypervirulent Klebsiella pneumoniae (hvKp) and Klebsiella variicola (hvKv) cause hospital/community-acquired infections, often associated with antimicrobial resistance (AMR). This study aimed to investigate the molecular epidemiology of hvKp and hvKv in northern Japan. Methods: A total of 500 K. pneumoniae and 421 K. variicola clinical isolates collected from August to December 2021 were studied. Prevalence of virulence factor-encoding genes, wzi sequence and associated K/KL type, sequence type (ST), and beta-lactamases and their types were characterized. Results: Any virulence gene (rmpA, rmpA2, peg-344, iucA, iutA, and iroB) and/or magA was detected in 25% (n = 125) of K. pneumoniae and 1% (n = 5) of K. variicola. Among these hvKp/hvKv, 22 wzi types (18 and 4 types, respectively) and 24 STs (20 and 4 STs, respectively) were identified. Sequence types of hvKp were classified into some clonal groups (CGs), among which CG35, including six STs, was the most common (n = 59; 47%), followed by CG23, and CG65. ST268 (CG35) associated with wzi95-K20 or wzi720 was the dominant lineage (n = 43, 34%), while K1:ST23/ST249 and K2:ST65/ST86 accounted for 26% and 13% of hvKp, respectively. Extended-spectrum beta-lactamase (ESBL) genes (blaCTX-M-2, blaCTX-M-3, blaCTX-M-15, and blaCTX-M-27) were detected in only ST23 and CG35 (ST268 and ST412) hvKp. No isolate was resistant to carbapenems, without detection of the ESBL gene in K. variicola. Phylogenetically, wzi was differentiated into two main clusters of K. pneumoniae and K. variicola. A major clonal group CG347 was identified in K. variicola. Conclusion: Clonal structures were revealed for hvKp and hvKv clinical isolates with their AMR status in northern Japan.

    DOI PubMed

  • Spread of multidrug resistance in non-PCV13/PCV20 serotypes of Streptococcus pneumoniae: A cross-sectional study ten years after the introduction of pneumococcal conjugate vaccine in Japan.

    Mitsuyo Kawaguchiya, Noriko Urushibara, Meiji Soe Aung, Nobuhide Ohashi, Rika Takamatsu, Sho Tsutida, Masahiko Ito, Nobumichi Kobayashi

    Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi   56 ( 6 ) 1288 - 1292  2023.12  [International journal]

    　View Summary

    Ten years after the introduction of the pneumococcal conjugate vaccine (PCV) in Japan, the prevalence rates of non-PCV13 and non-PCV20 serotypes among pediatric pneumococcal isolates were 94.0% and 73.7%, respectively. The predominant non-PCV13/PCV20 serotypes (15A, 35B, and 23A) were mostly multidrug-resistant (≥80.5%), exhibiting non-susceptibility to penicillin.

    DOI PubMed

  • Prevalence, clonal diversity, and antimicrobial resistance of hypervirulent Klebsiella pneumoniae and Klebsiella variicola clinical isolates in northern Japan.

    Norifumi Matsuda, Meiji Soe Aung, Noriko Urushibara, Mitsuyo Kawaguchiya, Nobuhide Ohashi, Kenji Taniguchi, Kenji Kudo, Masahiko Ito, Nobumichi Kobayashi

    Journal of global antimicrobial resistance   35   11 - 18  2023.12  [International journal]

    　View Summary

    OBJECTIVES: Hypervirulent Klebsiella pneumoniae (hvKp) and Klebsiella variicola (hvKv) cause hospital/community-acquired infections, often associated with antimicrobial resistance (AMR). This study aimed to investigate the molecular epidemiology of hvKp and hvKv in northern Japan. METHODS: A total of 500 K. pneumoniae and 421 K. variicola clinical isolates collected from August to December 2021 were studied. Prevalence of virulence factor-encoding genes, wzi sequence and associated K/KL type, sequence type (ST), and beta-lactamases and their types were characterized. RESULTS: Any virulence gene (rmpA, rmpA2, peg-344, iucA, iutA, and iroB) and/or magA was detected in 25% (n = 125) of K. pneumoniae and 1% (n = 5) of K. variicola. Among these hvKp/hvKv, 22 wzi types (18 and 4 types, respectively) and 24 STs (20 and 4 STs, respectively) were identified. Sequence types of hvKp were classified into some clonal groups (CGs), among which CG35, including six STs, was the most common (n = 59; 47%), followed by CG23, and CG65. ST268 (CG35) associated with wzi95-K20 or wzi720 was the dominant lineage (n = 43, 34%), while K1:ST23/ST249 and K2:ST65/ST86 accounted for 26% and 13% of hvKp, respectively. Extended-spectrum beta-lactamase (ESBL) genes (blaCTX-M-2, blaCTX-M-3, blaCTX-M-15, and blaCTX-M-27) were detected in only ST23 and CG35 (ST268 and ST412) hvKp. No isolate was resistant to carbapenems, without detection of the ESBL gene in K. variicola. Phylogenetically, wzi was differentiated into two main clusters of K. pneumoniae and K. variicola. A major clonal group CG347 was identified in K. variicola. CONCLUSION: Clonal structures were revealed for hvKp and hvKv clinical isolates with their AMR status in northern Japan.

    DOI PubMed

  • Genome analysis of an SCCmec element in ST9-MRSA from Myanmar with a unique mec gene complex and two ccr gene complexes (ccrA1B1 and ccrA5B7).

    Noriko Urushibara, Meiji Soe Aung, Mitsuyo Kawaguchiya, Nobuhide Ohashi, Nobumichi Kobayashi

    The Journal of antimicrobial chemotherapy    2023.05  [International journal]

    DOI PubMed

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Misc 【 display / non-display 】

  • ミャンマー、バングラデシュにおける病原細菌の薬剤耐性 現状と今後の課題

    小林 宣道, メイジ・ソウ・アウン, 漆原 範子, 川口谷 充代, ウィン・ウィン・モウ, ティダ・サン, シャマル・パウル

    日本衛生学雑誌 ( (一社)日本衛生学会 )  77 ( Suppl. ) S149 - S149  2022.03

  • ミャンマーにて分離されたST9-MRSAの全ゲノム解析

    漆原 範子, アウン・メイジソウ, 川口谷 充代, 小林 宣道

    日本衛生学雑誌 ( (一社)日本衛生学会 )  77 ( Suppl. ) S224 - S224  2022.03

  • 北海道における無莢膜型Streptococcus pneumoniaeのクローン系統と抗菌薬耐性(Clonal lineages and antimicrobial resistance of nonencapsulated Streptococcus pneumoniae in Hokkaido)

    川口谷 充代, 漆原 範子, Aung Meiji Soe, 工藤 兼司, 伊藤 政彦, 小林 宣道

    日本細菌学雑誌 ( 日本細菌学会 )  77 ( 1 ) 59 - 59  2022.02

  • 2011〜2019年に分離された無莢膜型肺炎球菌の薬剤感受性と分布率に関する疫学調査

    川口谷 充代, 小林 宣道

    日本公衆衛生学会総会抄録集 ( 日本公衆衛生学会 )  80回   440 - 440  2021.11

  • 北海道における新規ブドウ球菌種Staphylococcus argenteusの分子疫学的解析

    小林 宣道, アウン・メイジソウ, 川口谷 充代, 漆原 範子, 伊藤 政彦, 幅寺 敏

    日本衛生学雑誌 ( (一社)日本衛生学会 )  76 ( Suppl. ) S182 - S182  2021.03

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Research Projects 【 display / non-display 】

  • Molecular epidemiology and comprehensive exploration of novel virulence factors and their action mechanisms of hypervirulent Staphylococcus in Asia

    Fund for the Promotion of Joint International Research (International Collaborative Research)

    Project Year :

    2023.09
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    2027.03
     

    小林 宣道, 漆原 範子, 川口谷 充代, アウン メイジソウ, 大橋 伸英

  • ワクチン普及後に拡大した新興の多剤耐性肺炎球菌感染症制御のための分子疫学的研究

    基盤研究(C)

    Project Year :

    2022.04
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    2025.03
     

    川口谷 充代, 小林 宣道

  • Molecular epidemiological study of novel species and emerging drug-resistant staphylococcus in Japan and Asia, and basic construction of their infection control

    Grant-in-Aid for Scientific Research (B)

    Project Year :

    2020.04
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    2024.03
     

    小林 宣道, アウン メイジソウ, 川口谷 充代, 漆原 範子

    　View Summary

    本研究の目的は、薬剤耐性および臨床検査の面で近年注目されている新規ブドウ球菌種S.argenteus、市中感染型MRSA（CA-MRSA）、抗MRSA薬耐性菌等について、その疫学的状況を明らかにし感染対策の方策を探ることである。今年度は北海道において分離されたS.argenteus 82株、血液由来CA-MRSA 277株を対象として、分子疫学的解析を行った。S.argenteusは3つの遺伝子型ST1223、ST2198、ST2250に分類され、ST2250が最も多かった。STにより病原因子、特にエンテロトキシン（様）遺伝子と、薬剤耐性に違いが認められた。ST2198株は、他の2つのSTに比べ薬剤耐性遺伝子の検出率および一部薬剤への耐性率が有意に高かった。Panton-Valentine leukocidin(PVL)陽性菌、SCCmecIVcを有するメチシリン耐性菌がST2250において1株ずつ同定された。血液由来CA-MRSAでは、SCCmecIIaを保有するST5/ST764/ST2389が最も多く、次いで多かったのはSCCmecIVa、IVlを有する株であった。米国で優勢なUSA300と同じ特徴（SCCmecIVa/ST8/PVL+/ACME+）の株が全体の5.1％を占めており、以前のデータと比較して増加の傾向が示唆された。一方、PVLを保有しないSCCmecIVa MRSAが64株（23％）検出され、それらは多様な遺伝子型に属していた。USA300クローンが典型的に有するspeG遺伝子が、ST764-SCCmecIIaのMRSA2株に存在することが判明し、新たな特徴を持つMRSAが出現していることが示唆された。

  • 蛋白結合型ワクチン導入後に分離された 無莢膜型肺炎球菌の薬剤耐性と分子疫学的特徴

    Project Year :

    2019
    -
    2021
     

    川口谷充代

    Authorship： Principal investigator

  • Molecular epidemiology of Staphylococcus aureus, MRSA having novel enterotoxins and virulence factors

    Grant-in-Aid for Scientific Research (C)

    Project Year :

    2018.04
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    2021.03
     

    Aung Meiji Soe

    　View Summary

    Prevalence of novel staphylococcal enterotoxin (SE)(-like) toxin genes in Staphylococcus aureus and its relative species S. argenteus, and their molecular epidemiological characteristics were analyzed. selw and selx were detected in more than 80% of S. aureus isolates examined, and showed diversity in their nucleotide sequences. S. aureus harbored sey and selz at low rate, without having sel26 and sel27. The sey gene was detected in all the ST2250 S. argenteus isolates, and more than half of this lineage harbored selz, sel26, and sel27. Prevalence of novel SE(-like) genes was revealed to be different depending on lineages of S. aureus and S. argenteus.

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Teaching Experience 【 display / non-display 】

  • 衛生学  

    札幌医科大学  

  • 基礎配属  

    札幌医科大学  

  • 医学英語  

    札幌医科大学  

  • 社会医学実習  

    札幌医科大学  

  • 小学校全教科  

    札幌市公立小学校  

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