研究者詳細 - 齋藤　悠城

論文 - 齋藤　悠城

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Role of cellular senescence in inflammation and regeneration

Yuki Saito, Sena Yamamoto, Takako S. Chikenji

Inflammation and Regeneration ( Springer Science and Business Media LLC ) 44 ( 1 ) 2024年06月 [査読有り] [招待有り]

担当区分：筆頭著者

Abstract Cellular senescence is the state in which cells undergo irreversible cell cycle arrest and acquire diverse phenotypes. It has been linked to chronic inflammation and fibrosis in various organs as well as to individual aging. Therefore, eliminating senescent cells has emerged as a potential target for extending healthy lifespans. Cellular senescence plays a beneficial role in many biological processes, including embryonic development, wound healing, and tissue regeneration, which is mediated by the activation of stem cells. Therefore, a comprehensive understanding of cellular senescence, including both its beneficial and detrimental effects, is critical for developing safe and effective treatment strategies to target senescent cells. This review provides an overview of the biological and pathological roles of cellular senescence, with a particular focus on its beneficial or detrimental functions among its various roles.

DOI

Senescence-associated secretory phenotypes in mesenchymal cells contribute to cytotoxic immune response in oral lichen planus.

Shogo Ijima, Yuki Saito, Sena Yamamoto, Kentaro Nagaoka, Taiki Iwamoto, Arisa Kita, Maki Miyajima, Tsukasa Sato, Akihiro Miyazaki, Takako S Chikenji

Immunity & ageing : I & A 20 ( 1 ) 72 - 72 2023年12月 [査読有り] [国際誌]

担当区分：責任著者

Oral lichen planus is a chronic inflammatory condition that adversely affects the oral mucosa; however, its etiology remains elusive. Consequently, therapeutic interventions for oral lichen planus are limited to symptomatic management. This study provides evidence of the accumulation of senescent mesenchymal cells, CD8 + T cells, and natural killer cells in patients with oral lichen planus. We profiled the patients' tissues using the National Center for Biotechnology Information Gene Expression Omnibus database and found that senescence-related genes were upregulated in these tissues by gene set enrichment analysis. Immunohistochemical analysis showed increased senescent mesenchymal cells in the subepithelial layer of patients with oral lichen planus. Single-cell RNA-seq data retrieved from the Gene Expression Omnibus database of patients with oral lichen planus revealed that mesenchymal cells were marked by the upregulation of senescence-related genes. Cell-cell communication analysis using CellChat showed that senescent mesenchymal cells significantly influenced CD8 + T cells and natural killer cells via CXCL12-CXCR4 signaling, which is known to activate and recruit CD8 + T cells and NK cells. Finally, in vitro assays demonstrated that the secretion of senescence-associated factors from mesenchymal cells stimulated the activation of T cells and natural killer cells and promoted epithelial cell senescence and cytotoxicity. These findings suggest that the accumulation of mesenchymal cells with senescence-associated secretory phenotype may be a key driver of oral lichen planus pathogenesis.

DOI PubMed

Intrathecal Injection of Mesenchymal Stromal Cell Cultured on 3D Fiber Ameliorates Multiple Organ Damage in Murine Lupus

Yuki Saito, Maki Miyajima, Sena Yamamoto, Norihiro Miura, Tsukasa Sato, Arisa Kita, Shogo Ijima, Mineko Fujimiya, Takako S Chikenji

Stem Cells Translational Medicine ( Oxford University Press (OUP) ) 2022年04月 [査読有り]

担当区分：筆頭著者

Abstract Up to 60% of patients with systemic lupus erythematosus (SLE) experience autonomic symptom. Sympathetic nervous system damage can cause dysfunction of the bone marrow that activates inflammatory cells, potentially causing multiple organ damage. We hypothesized that sympathetic nervous system damage would induce bone marrow dysfunction with multiple organ damage in SLE, and that multiple organ damage could be improved by therapy targeting the nervous system. Here, we showed that damage to autonomic nerves and Schwann cells occurred in the bone marrow and central nervous system of SLE model mice. A neurotoxic drug increased mortality and induced severe neuropathy and multiple organ damage, while a neuroprotective drug prevented multiple organ damage. The administration of bone marrow-derived mesenchymal stromal cells (BMSCs) cultured on a 3-dimensional fiber scaffold improved bone marrow neuropathy, skin lesions, kidney function, and mortality. Our results reveal that bone marrow neuropathy influence multiple organ damage associated with SLE, and improvement of bone marrow neuropathy by intrathecal injection of BMSC may be a target for SLE multiple-organ damage.

DOI

Altered regulation of mesenchymal cell senescence in adipose tissue promotes pathological changes associated with diabetic wound healing.

Arisa Kita, Yuki Saito, Norihiro Miura, Maki Miyajima, Sena Yamamoto, Tsukasa Sato, Takatoshi Yotsuyanagi, Mineko Fujimiya, Takako S Chikenji

Communications biology 5 ( 1 ) 310 - 310 2022年04月 [査読有り] [国際誌]

担当区分：責任著者

Pathologic diabetic wound healing is caused by sequential and progressive deterioration of hemostasis, inflammation, proliferation, and resolution/remodeling. Cellular senescence promotes wound healing; however, diabetic wounds exhibit low levels of senescent factors and accumulate senescent cells, which impair the healing process. Here we show that the number of p15INK4B + PDGFRα + senescent mesenchymal cells in adipose tissue increases transiently during early phases of wound healing in both non-diabetic mice and humans. Transplantation of adipose tissue from diabetic mice into non-diabetic mice results in impaired wound healing and an altered cellular senescence-associated secretory phenotype (SASP), suggesting that insufficient induction of adipose tissue senescence after injury is a pathological mechanism of diabetic wound healing. These results provide insight into how regulation of senescence in adipose tissue contributes to wound healing and could constitute a basis for developing therapeutic treatment for wound healing impairment in diabetes.

DOI PubMed

Fisetin reduces the senescent tubular epithelial cell burden and also inhibits proliferative fibroblasts in murine lupus nephritis.

Shogo Ijima, Yuki Saito, Kentaro Nagaoka, Sena Yamamoto, Tsukasa Sato, Norihiro Miura, Taiki Iwamoto, Maki Miyajima, Takako S Chikenji

Frontiers in immunology 13 960601 - 960601 2022年 [査読有り] [国際誌]

担当区分：責任著者

Systemic lupus erythematosus (SLE) is a chronic autoimmune inflammatory disease characterized by the involvement of multiple organs. Lupus nephritis (LN) is a major risk factor for overall morbidity and mortality in SLE patients. Hence, designing effective drugs is pivotal for treating individuals with LN. Fisetin plays a senolytic role by specifically eliminating senescent cells, inhibiting cell proliferation, and exerting anti-inflammatory, anti-oxidant, and anti-tumorigenic effects. However, limited research has been conducted on the utility and therapeutic mechanisms of fisetin in chronic inflammation. Similarly, whether the effects of fisetin depend on cell type remains unclear. In this study, we found that LN-prone MRL/lpr mice demonstrated accumulation of Ki-67-positive myofibroblasts and p15INK4B-positive senescent tubular epithelial cells (TECs) that highly expressed transforming growth factor β (TGF-β). TGF-β stimulation induced senescence of NRK-52E renal TECs and proliferation of NRK-49F renal fibroblasts, suggesting that TGF-β promotes senescence and proliferation in a cell type-dependent manner, which is inhibited by fisetin treatment in vitro. Furthermore, fisetin treatment in vivo reduced the number of senescent TECs and myofibroblasts, which attenuated kidney fibrosis, reduced senescence-associated secretory phenotype (SASP) expression, and increased TEC proliferation. These data suggest that the effects of fisetin vary depending on the cell type and may have therapeutic effects in complex and diverse LN pathologies.

DOI PubMed

Accumulation of Senescent Neural Cells in Murine Lupus With Depression-Like Behavior

Yuki Saito, Maki Miyajima, Sena Yamamoto, Tsukasa Sato, Norihiro Miura, Mineko Fujimiya, Takako S. Chikenji

Frontiers in Immunology ( Frontiers Media SA ) 12 692321 - 692321 2021年11月 [査読有り] [国際誌]

担当区分：筆頭著者

Neuropsychiatric manifestations targeting the central, peripheral, and autonomic nervous system are common in systemic lupus erythematosus (SLE); collectively, these symptoms are termed neuropsychiatric SLE (NPSLE). Among a wide variety of neuropsychiatric symptoms, depression is observed in about 24-39% of SLE patients. Several cytokines and chemokines have been identified as biomarkers or therapeutic targets of NPSLE; in particular, the levels of type 1 interferons, TNFs, and IL-6 are elevated in SLE patient’s cerebrospinal fluid (CSF), and these factors contribute to the pathology of depression. Here, we show that senescent neural cells accumulate in the hippocampal cornu ammonis 3 (CA3) region in MRL/lpr SLE model mice with depressive behavior. Furthermore, oral administration of fisetin, a senolytic drug, reduced the number of senescent neural cells and reduced depressive behavior in the MRL/lpr mice. In addition, transcription of several senescence and senescence-associated secretory phenotype (SASP) factors in the hippocampal region also decreased after fisetin treatment in the MRL/lpr mice. These results indicate that the accumulation of senescent neural cells in the hippocampus plays a role in NPSLE pathogenesis, and therapies targeting senescent cells may represent a candidate approach to treat NPSLE.

DOI PubMed

Diverse Roles of Cellular Senescence in Skeletal Muscle Inflammation, Regeneration, and Therapeutics.

Yuki Saito, Takako S. Chikenji

Frontiers in Pharmacology ( Frontiers Media SA ) 12 2021年09月 [査読有り] [招待有り]

担当区分：筆頭著者

Skeletal muscle undergoes vigorous tissue remodeling after injury. However, aging, chronic inflammatory diseases, sarcopenia, and neuromuscular disorders cause muscle loss and degeneration, resulting in muscular dysfunction. Cellular senescence, a state of irreversible cell cycle arrest, acts during normal embryonic development and remodeling after tissue damage; when these processes are complete, the senescent cells are eliminated. However, the accumulation of senescent cells is a hallmark of aging tissues or pathological contexts and may lead to progressive tissue degeneration. The mechanisms responsible for the effects of senescent cells have not been fully elucidated. Here, we review current knowledge about the beneficial and detrimental effects of senescent cells in tissue repair, regeneration, aging, and age-related disease, especially in skeletal muscle. We also discuss how senescence of muscle stem cells and muscle-resident fibro-adipogenic progenitors affects muscle pathologies or regeneration, and consider the possibility that immunosenescence leads to muscle pathogenesis. Finally, we explore senotherapy, the therapeutic targeting of senescence to treat age-related disease, from the standpoint of improving muscle regeneration.

DOI

Exercise enhances skeletal muscle regeneration by promoting senescence in fibro-adipogenic progenitors

Yuki Saito, Takako S. Chikenji, Takashi Matsumura, Masako Nakano, Mineko Fujimiya

Nature Communications 2020年02月 [査読有り]

担当区分：筆頭著者

DOI

Can an insole for obese individuals maintain the arch of the foot against repeated hyper loading?

Yuki Saito, Takako S. Chikenji, Yuichi Takata, Tomoaki Kamiya, Eiichi Uchiyama

BMC Musculoskeletal Disorders 2019年12月 [査読有り]

担当区分：筆頭著者 , 責任著者

DOI

p16INK4A-expressing mesenchymal stromal cells restore the senescence–clearance–regeneration sequence that is impaired in chronic muscle inflammation

Takako S. Chikenji, Yuki Saito, Naoto Konari, Masako Nakano, Yuka Mizue, Miho Otani, Mineko Fujimiya

EBioMedicine ( Elsevier {BV} ) 44 86 - 97 2019年06月 [査読有り]

DOI

Phosphorylated Platelet-Derived Growth Factor Receptor-Positive Cells With Anti-apoptotic Properties Accumulate in the Synovium of Patients With Rheumatoid Arthritis

Takashi Matsumura, Yuki Saito, Tomoyuki Suzuki, Atsushi Teramoto, Yasuhiro Ozasa, Toshihiko Yamashita, Mineko Fujimiya, Takako Saito-Chikenji

Frontiers in Immunology ( Frontiers Media {SA} ) 10 2019年02月 [査読有り]

DOI

PDGFR Signaling Mediates Hyperproliferation and Fibrotic Responses of Subsynovial Connective Tissue Cells in Idiopathic Carpal Tunnel Syndrome

Yuki Saito, Takako Chikenji, Yasuhiro Ozasa, Mineko Fujimiya, Toshihiko Yamashita, Anne Gingery, Kousuke Iba

Scientific Reports ( Springer Nature ) 7 ( 1 ) 2017年12月 [査読有り]

担当区分：筆頭著者

DOI

Cellular senescence and wound healing in aged and diabetic skin

Arisa Kita, Sena Yamamoto, Yuki Saito, Takako S. Chikenji

Frontiers in Physiology 15 2024年02月 [査読有り] [招待有り]

担当区分：責任著者

【健康寿命の鍵を握る骨格筋代謝・内分泌を介した全身性制御の分子基盤から運動による抗老化まで】(第3章)個体の老化を支配する骨格筋の老化運動による細胞老化制御と骨格筋再生

齋藤悠城, 千見寺貴子

実験医学 ( (株)羊土社 ) 40 ( 2 ) 222 - 228 2022年02月

細胞老化はがん抑制や個体発生、さらには組織再生など生体に重要な機能を果たす一方で、加齢や慢性炎症などを進展する二面性が報告されている。近年、骨格筋への運動ストレスが細胞老化を誘導して骨格筋の修復や再生を促進するだけでなく、慢性炎症や線維化など骨格筋病態へも関与することが明らかになってきた。細胞老化制御機構の解明は新たな骨格筋治療の突破口になる可能性を秘めている。本稿では、運動による骨格筋の細胞老化と組織修復プロセス、また加齢性疾患における細胞老化の影響、細胞老化を標的とした新規治療法の可能性などについて概説する。(著者抄録)

Flexor hallucis longus tendinous slips and the relationship to toe flexor strength.

Kento Hirota, Kota Watanabe, Atsushi Teramoto, Yuki Saito, Masaki Katayose

Foot and ankle surgery : official journal of the European Society of Foot and Ankle Surgeons 27 ( 8 ) 851 - 854 2021年12月 [国際誌]

BACKGROUND: In the general population, the flexor hallucis longus (FHL) often has tendinous slips to lesser toes and the number of FHL slips varies between individuals. The purpose of this study was to investigate the relationship between the number of FHL tendinous slips in an individual foot and its toe flexor strength. METHODS: Forty healthy men were included in the study. The FHL branch test was used to assess each subject for the number of FHL tendinous slips. Toe flexor strength in each toe was measured using a force gauge. A two-way ANOVA was used to compare toe flexor strength between groups classified according to the number of FHL slips. RESULTS: The group of subjects with FHL branching to the second toe was the most common (20/40). The toe flexor strength ratio of the third toe was significantly lower in feet lacking FHL branching to the third toe than in those feet which did have branching to the third toe (P = 0.005). CONCLUSIONS: Toe flexor strength was affected by FHL tendinous slips. Considering the number of the FHL tendinous slips an individual foot has may be useful in clinical practice for rehabilitation or training of toe flexor muscles.

DOI PubMed

An enriched environment prevents cognitive impairment in an Alzheimer’s disease model by enhancing the secretion of exosomal microRNA-146a from the choroid plexus

Masako Nakano, Kenta Kubota, Shin Hashizume, Eiji Kobayashi, Takako S. Chikenji, Yuki Saito, Mineko Fujimiya

Brain, Behavior, & Immunity - Health ( Elsevier BV ) 9 100149 - 100149 2020年12月

DOI

Eccentric resistance training ameliorates muscle weakness in a mouse model of idiopathic inflammatory myopathies.

Koichi Himori, Yuki Ashida, Daisuke Tatebayashi, Masami Abe, Yuki Saito, Takako Chikenji, Håkan Westerblad, Daniel C Andersson, Takashi Yamada

Arthritis & rheumatology (Hoboken, N.J.) 73 ( 5 ) 848 - 857 2020年11月 [国際誌]

OBJECTIVE: High-force eccentric contractions (ECCs) have traditionally been excluded from rehabilitation programs of patients with idiopathic inflammatory myopathies (IIMs) due to unverified fear of causing muscle damage and inflammation. Here, we used an IIM animal model, experimental autoimmune myositis (EAM) mice, to investigated whether ECC training can be safely and effectively used to counteract muscle weakness in IIM. METHODS: EAM was induced in Balb/c mice by immunization with three injections of myosin emulsified in complete Freund's adjuvant. Control (CNT: n=12) and EAM (n=12) mice were exposed to an acute bout of 100 ECCs or 4 weeks of ECC training (20 ECCs every other day). To induce ECCs, plantar flexor muscles were electrically stimulated while the ankle was forcibly dorsiflexed. RESULTS: Less cell damage, as assessed by Evans blue dye uptake, was observed in EAM than in CNT muscles after an acute bout of 100 ECCs (P < 0.05). Maximum Ca2+ -activated force was decreased in skinned gastrocnemius muscle fibers from EAM mice and this was accompanied by increased expression of the endoplasmic reticulum (ER) stress proteins glucose-regulated protein 78 and 94 (P < 0.05). ECC training prevented the force decrease and the increase in ER stress proteins, and also enhanced the expression and myofibrillar binding of small heat shock proteins (sHSP) (P < 0.05), which can stabilize myofibrillar structure and function. CONCLUSION: ECC training protected against the reduction in myofibrillar force generating capacity in an IIM mouse model and this occurred via inhibition of ER stress responses and sHSP-mediated myofibrillar stabilization.

DOI PubMed

足趾屈筋群の付着部とその相互関係

上村杏菜, 渡邉耕太, 廣田健斗, 齋藤悠城

日本臨床スポーツ医学会誌 ( (一社)日本臨床スポーツ医学会 ) 28 ( 3 ) 441 - 446 2020年08月

長母趾屈筋(以下FHL)、長趾屈筋(以下FDL)、足底方形筋(以下QP)には筋の付着部に個人差がある。各筋についての解剖研究はあるが、3筋の相互関係は不明である。本研究では足底におけるFHL腱とFDL腱の解剖学的バリエーションごとのQPの付着部を調査した。日本人解剖標本38体50足でFHL腱とFDL腱の関係、FHLが分枝する足趾、QPの付着部について肉眼解剖をし、QPの付着部データをFHL分枝別に解析した。FHL腱とFDL腱の関係では、FHL腱からFDL腱へ分枝するものが96%であった。FHLの分枝については、第2、3趾へ分枝するものが50%と最多であった。QPは96%でFDL腱とFHL腱に付着し、FHLの分枝にも高率に付着していた。またFHL分枝数の違いにかかわらず、QPが第5趾のFDL腱に付着する割合は低率であった。本研究結果から、QPはFDL腱だけでなくFHL分枝にも付着し、それらの機能を補助する例が多いことが示された。また、FHLの分枝数が足趾屈曲機能に大きな影響を与える可能性がある。(著者抄録)

Tensile load on the flexor digitorum profundus tendon during palmar and lateral blocking exercises: Influence on blocking force and distal interphalangeal joint flexion angle.

Yukihiro Osanami, Mitsuhiro Aoki, Rikiya Shirato, Yuki Saito, Egi Hidaka, Hiroki Miyamoto, Eiichi Uchiyama

Journal of hand therapy : official journal of the American Society of Hand Therapists 2020年07月 [査読有り] [国際誌]

STUDY DESIGN: This is a basic science research. INTRODUCTION: Isolating excursion of the flexor digitorum profundus (FDP) in zones I and II is common practice in the current management after flexor tendon repair. During this procedure, the proximal interphalangeal joint is sometimes fully extended with unmeasured external forces at the middle phalanx when the distal interphalangeal joint is actively flexed. PURPOSE OF THE STUDY: The purpose of the study was to investigate the incremental effect of external force with palmar blocking versus lateral blocking and increased angles of flexion on internal tendon forces at the repair site for a safer application of force by the treating therapist. METHODS: Eight human cadaveric fingers were studied. To simulate palmar or lateral finger blocking, a compression force of blocking was applied from 5N (510 grams) to 25N (2,550 grams) on the skin surface of the palmar or the lateral aspect of each of these middle phalanges in 5N increments. The tensile load on the FDP tendon during distal interphalangeal joint flexion from 0° to 60° was measured in 10° increments. RESULTS: During palmar blocking, the tensile load was significantly increased with increases in palmar blocking force. However, no significant increase in the tensile load on the FDP tendon was observed at any lateral blocking. DISCUSSION: Lateral blocking exercise can be performed with less tensile force on the FDP tendon when performing blocking exercise after flexor tendon injury repair. CONCLUSIONS: This study supports the concept that lateral blocking with incremental joint angles allows a safer application of force for the healing tendon.

DOI PubMed

Bone marrow-derived mesenchymal stem cells improve cognitive impairment in an Alzheimer's disease model by increasing the expression of microRNA-146a in hippocampus.

Masako Nakano, Kenta Kubota, Eiji Kobayashi, Takako S Chikenji, Yuki Saito, Naoto Konari, Mineko Fujimiya

Scientific reports 10 ( 1 ) 10772 - 10772 2020年07月 [査読有り] [国際誌]

Alzheimer's disease (AD) is characterized by the accumulation of amyloid-β and tau. We previously reported that administration of bone marrow mesenchymal stem cells (BM-MSCs) ameliorates diabetes-induced cognitive impairment by transferring exosomes derived from these cells into astrocytes. Here, we show that intracerebroventricularly injected BM-MSCs improve cognitive impairment in AD model mice by ameliorating astrocytic inflammation as well as synaptogenesis. Although AD model mice showed an increase in NF-κB in the hippocampus, BM-MSC-treated AD model mice did not show this increase but showed an increase in levels of microRNA (miR)-146a in the hippocampus. Intracerebroventricularly injected BM-MSCs were attached to the choroid plexus in the lateral ventricle, and thus, BM-MSCs may secrete exosomes into the cerebrospinal fluid. In vitro experiments showed that exosomal miR-146a secreted from BM-MSCs was taken up into astrocytes, and an increased level of miR-146a and a decreased level of NF-κB were observed in astrocytes. Astrocytes are key cells for the formation of synapses, and thus, restoration of astrocytic function may have led to synaptogenesis and correction of cognitive impairment. The present study indicates that exosomal transfer of miR-146a is involved in the correction of cognitive impairment in AD model mice.

DOI PubMed

Flexor hallucis longus tendon branch test: Development and validation of a new method to assess anatomical variation of the tendinous slip

Kento Hirota, Kota Watanabe, Yuki Saito, Masaki Katayose

Foot and Ankle Surgery ( Elsevier {BV} ) 2019年08月 [査読有り]

DOI

長母趾屈筋の機能 FHL分枝テストの開発

廣田健斗, 渡邉耕太, 齋藤悠城, 片寄正樹, 寺本篤史, 山下敏彦

整形・災害外科 ( 金原出版(株) ) 61 ( 7 ) 861 - 868 2018年06月 [査読有り]

＜文献概要＞生体において長母趾屈筋(FHL)分枝パターンを判別するテスト方法を考案し,その妥当性を検討した。本テストは足関節中間位にて母趾趾節間関節の自動屈曲運動を行わせ,同時に屈曲した足趾を記録する方法である。1)機能解剖学的検証:Thiel法固定足標本6肢を用いた。FHL,長趾屈筋(FDL)をそれぞれ牽引し,各趾末節骨に挿入した鋼線の屈曲角度変化量を算出し,その後,FHLの分枝を肉眼的に観察した。2)生体による検証:健常男性4名に対しワイヤー電極を介してFHLを電気刺激し,電気刺激による屈曲趾とFHL分枝テストの一致度を評価した。1)FHL分枝の付着する足趾では,FHL牽引時の足趾屈曲角度変化量がFDL牽引時と同等であった。2)電気刺激による屈曲趾とFHL分枝テスト中に屈曲した足趾は全被験者で一致していた。FHLの収縮時にはFHL分枝が付着する足趾も十分に屈曲することが示された。今回考案したテストはFHLの分枝判別に利用できると考えられた。

長母趾屈筋の機能 FHL分枝テストの開発

廣田健斗, 渡邉耕太, 片寄正樹, 齋藤悠城, 山下敏彦

北海道整形災害外科学会雑誌 ( 北海道整形災害外科学会 ) 59 ( 2 ) 296 - 297 2018年03月

Thiel法固定足標本を用いた長母趾屈筋、長趾屈筋牽引時の足趾屈曲角度変化

廣田健斗, 渡邉耕太, 齋藤悠城

日本足の外科学会雑誌 ( (一社)日本足の外科学会 ) 38 ( 1 ) 59 - 62 2017年09月

長母趾屈筋(FHL)腱は母趾のみでなくその分枝によって他の趾にも付着するが、FHLの分枝が足趾屈曲機能に与える影響は不明である。そこで、ヒト足標本を用いてFHLと長趾屈筋(FDL)牽引時の足趾屈曲角度変化量を比較した。対象は関節可動性を温存するThiel法固定足標本5肢。足関節中間位にてFHL、FDLを個別に牽引した際の足趾屈曲運動をビデオカメラで撮影した。各趾末節骨に挿入した鋼線の角度を計測し、牽引前の角度から各牽引距離までの角度変化量を算出した。牽引終了後、FHLの分枝を肉眼解剖にて観察した。第2-5趾において、各筋牽引時の足趾屈曲角度変化量を比較した。FHLは全標本で第1、2、3趾に付着していた。FHLの分枝が付着していた第2、3趾では、FHLとFDL牽引間で角度変化量に差はなかった。一方第4、5趾では、FHL牽引時の角度変化量はFDL牽引時よりも小さかった。第2、3趾に付着するFHLの分枝は、FHLの収縮時にFDLと同等の足趾屈曲機能を発揮する可能性が示された。(著者抄録)

Bone marrow-derived mesenchymal stem cells improve diabetes-induced cognitive impairment by exosome transfer into damaged neurons and astrocytes

Masako Nakano, Kanna Nagaishi, Naoto Konari, Yuki Saito, Takako Chikenji, Yuka Mizue, Mineko Fujimiya

Scientific Reports ( Springer Science and Business Media {LLC} ) 6 ( 1 ) 2016年04月 [査読有り]

DOI

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