Details of a Researcher - YASUDA Mitsuru

Papers - YASUDA Mitsuru

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Neisseria meningitidis(髄膜炎菌)Y群による男子尿道炎の1例

大楠清文, 安田満, 大塚喜人, 河村好章, 江崎孝行, 出口隆

感染症学雑誌 ( (一社)日本感染症学会 ) 79 ( 9 ) 797 - 797 2005.09

Cultivation of Neisseria gonorrhoeae in liquid media and determination of its in vitro susceptibilities to quinolones

M Takei, Y Yamaguchi, H Fukuda, M Yasuda, T Deguchi

JOURNAL OF CLINICAL MICROBIOLOGY ( AMER SOC MICROBIOLOGY ) 43 ( 9 ) 4321 - 4327 2005.09

The cultivation of Neisseria gonorrhoeae by use of fastidious broth (FB) was evaluated. FB was found to be able to support the growth of all N. gonorrhoeae strains tested in this study without a rapid decrease in the viable count after exponential growth. After 24 h of incubation at 35 degrees C with 5% CO2, viable counts of all strains reached over 10(8) CFU/ml in FB. Similar growth of the wild-type strain and its target-altered quinolone-resistant derivatives was observed. The susceptibilities of laboratory-adapted strains and clinical isolates to quinolones were tested by the microdilution method using FB. The MICs determined by microdilution were not significantly different from those determined by the agar dilution method recommended by the CLS1 (formerly National Committee for Clinical Laboratory Standards). Moreover, the concentration-dependent time-kill of quinolones such as gatifloxacin and ciprotioxacin was observed in FB. At 2 to 4 times the MIC, gatifloxacin and ciprofloxacin were predominantly bactericidal against N. gonorrhoeae WHO A. At the MIC, the activities of both quinolones ranged from bactericidal to bacteriostatic. At 0.25 to 0.5 times the MIC, gonococcal growth was comparable to that of the growth control. These results suggest that the cultivation of N. gonorrhoeae by use of FB may be useful for evaluation of the antibacterial effects of quinolones.

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Polymerase chain reaction-based subtyping of Ureaplasma parvum and Ureaplasma urealyticum in first-pass urine samples from men with or without urethritis

T Yoshida, H Ishiko, M Yasuda, Y Takahashi, Y Nomura, Y Kubota, M Tamaki, S Maeda, T Deguchi

SEXUALLY TRANSMITTED DISEASES ( LIPPINCOTT WILLIAMS & WILKINS ) 32 ( 7 ) 454 - 457 2005.07

Background: Our previous study suggested a significant association between Ureaplasma urealyticum and nongonococcal urethritis (NGU). However, association of the serovars of U. urealyticum with NGU remains unclear. A polymerase chain reaction (PCR)-based assay can distinguish 4 serovars of Ureoplasma parvum from each other and categorize 10 serovars of U. urealyticum into 3 subtypes: subtype 1 (serovars 2, 5, 8, and 9), subtype 2 (serovars 4, 10, 12, and 13), and subtype 3 (serovars 7 and 11). Goal: The goal of this study was to determine which subtypes of U. urealyticum are associated with NGU as determined by PCR-based assay. Study: The prevalence of U. urealyticum subtypes in 106 ureaplasma-positive men with urethritis was compared with that in 30 ureaplasma-positive men without urethritis. Results: In men with nonchlamydial NGU and men with Mycoplasma genitalium-negative nonchlamydial NGU, only U. urealyticum subtype 1 (serovars 2, 5, 8, and 9) was detected significantly more often than in men without urethritis. Conclusion: This study suggests that subtype 1 of U. urealyticum (serovars 2, 5, 8, and 9) is associated with NGU independently of Chlamydia trachomatis or M. genitalium.

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Neisseria meningitidis(髄膜炎菌)Y群による男子尿道炎の1例

大楠清文, 安田満, 大塚喜人, 河村好章, 江崎孝行, 出口隆

感染症学雑誌 ( (一社)日本感染症学会 ) 79 ( 臨増 ) 307 - 307 2005.03

尿膜管臍瘻に対する腹腔鏡下手術

高橋義人, 横井繁明, 安田満, 出口隆

手術 59 1591 - 1597 2005.01

A Clinical Isolate of Leclercia adecarboxylata from a Patient of Pyelonephritis

SAWAMURA Haruki, KAWAMURA Yoshiaki, YASUDA Mitsuru, OHKUSU Kiyofumi, TAKAHASHI Yoshito, ISHIHARA Satoshi, DEGUCHI Takashi, EZAKI Takayuki

Journal of the Japanese Association for Infectious Diseases ( The Japanese Association for Infectious Diseases ) 79 ( 10 ) 831 - 835 2005.01

DOI CiNii

泌尿器科系感染症Neisseria meningitidis（髄膜炎菌）Serogroup Yによる男子尿道炎の一例

大楠清文, 萩原徳康, 河村好章, 安田満, 大塚喜人, 江崎孝行, 出口隆

症例から学ぶ感染症診療のポイント 137 - 142 2005.01

Emergence and spread of Neisseria gonorrhoeae clinical isolates harboring mosaic-like structure of penicillin-binding protein 2 in central Japan

M Ito, T Deguchi, KS Mizutani, M Yasuda, S Yokoi, SI Ito, Y Takahashi, S Ishihara, Y Kawamura, T Ezaki

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY ( AMER SOC MICROBIOLOGY ) 49 ( 1 ) 137 - 143 2005.01

Of 150 clinical isolates of Neisseria gonorrhoeae recovered in 2001, we examined 55 clinical isolates of N. gonorrhoeae for which cefixime MICs were greater than or equal to0.125 mug/ml and randomly selected 15 isolates for which cefixime MICs were less than or equal to0.06 mug/ml for analysis of alterations in the penicillin-binding protein 2 (PBP 2) gene. We found insertion of an extra codon (Asp-345a) in the transpeptidase domain of PBP 2, and this insertion occurred alone or in conjunction with other amino acid substitutions. We also found a mosaic PBP 2 that was composed of fragments of the PBP 2 proteins from Neisseria cinera and Neisseria perflava. This mosaic PBP 2 was significantly associated with decreased susceptibilities to penicillin and cephalosporins, especially oral cephalosporins. For most of the isolates with a mosaic PBP 2, the cefixime MICs were greater than or equal to0.5 mug/ml and the cefdinir MICs were greater than or equal to1 mug/ml. Analysis of chromosomal DNA restriction patterns by pulsed-field gel electrophoresis revealed that most isolates with the mosaic PBP 2 were genetically similar. The recombination events that generated the mosaic PBP 2 would likely have contributed to the decreased sensitivities to cephalosporins. Isolates with the mosaic PBP 2 appear to threaten the efficacy of the currently recommended regimen with cefixime. The emergence of such strains may be the result of the in vivo generation of clones in which interspecies recombination occurred between the penA genes of N. gonorrhoeae and commensal Neisseria species.

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腎不全症例に対する鏡視下腎摘除術の経験(第93回日本泌尿器科学会総会)

横井繁明, 中根慶太, 加藤卓, 水谷晃輔, 萩原徳康, 安田満, 仲野正博, 伊藤慎一, 江原英俊, 高橋義人, 石原哲, 出口隆

日本泌尿器科学会雑誌 ( 一般社団法人日本泌尿器科学会 ) 96 ( 2 ) 348 - 348 2005

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Mycoplasma genitalium urethritis in men

S Ishihara, M Yasuda, S Ito, S Maeda, T Deguchi

INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS ( ELSEVIER SCIENCE BV ) 24 S23 - S27 2004.09

Mycoplasma genitalium was first isolated from two men with non-gonococcal urethritis (NGU) and thereafter shown to produce urethritis in subhuman primates, inoculated intraurethrally. This mycoplasma has been detected significantly more often in patients with acute NGU, particularly in patients with non-chlamydial NGU, than in subjects without urethritis. The prevalence of M. genitalium-positive non-chlarnydial NGU ranges from 18 to 46% of all non-chlamydial NGU cases. In addition, the persistence of M. genitalium in the urethra after antimicrobial chemotherapy is associated with persistence or recurrence of NGU. The various results reported to date tend to support the proposition that M. genitalium is a pathogen of NGU. M. genitalium is highly susceptible to tetracyclines, macrolides, and some new fluoroquinol ones, but the clinical data on the chemotherapy in M. genitalium-positive NGU is extremely limited. Because of the possible association between the post-treatment presence of M. genitalium in the urethra and persistent or recurrent NGU, the eradication of this mycoplasma from the urethra is essential in the management of patients with M. genitalium-positive NGU. Further studies are required to establish the optimal chemotherapy for M. genitalium-positive NGU. (C) 2004 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.

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Remarkable increase in central Japan in 2001-2002 of Neisseria gonorrhoeae isolates with decreased susceptibility to penicillin, tetracycline, oral cephalosporins, and fluoroquinolones

M Ito, M Yasuda, S Yokoi, SI Ito, Y Takahashi, S Ishihara, SI Maeda, T Deguchi

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY ( AMER SOC MICROBIOLOGY ) 48 ( 8 ) 3185 - 3187 2004.08

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Association of Ureaplasma urealyticum (Biovar 2) with nongonococcal urethritis

T Deguchi, T Yoshida, T Miyazawa, M Yasuda, M Tamaki, H Ishiko, SI Maeda

SEXUALLY TRANSMITTED DISEASES ( LIPPINCOTT WILLIAMS & WILKINS ) 31 ( 3 ) 192 - 195 2004.03

Background: The tiny (T)-strain mycoplasmas, designated in 1974 as Ureaplasma urealyticum, have been divided into 2 species, Ureaplasma parvum (biovar 1) and U. urealyticum (biovar 2), but association of each of these species with nongonococcal urethritis (NGU) remains unclear. Goal: The goal of this study was to determine whether U. parvum (biovar 1) or U. urealyticum (biovar 2) is associated with NGU. Study Design: The prevalences of U. parpum (biovar 1) and U. urealyticum (biovar 2) in 572 patients with urethritis were compared with those in 141 men without urethritis. Results: The prevalence of U. urealyticum (biovar 2) in men with NGU (15.8%) or with nonchlamydial NGU (18.0%) was significantly higher than that in men without urethritis (7.8%). The prevalence of U. parvum (biovar 1) in men with NGU (8.5%) or with nonchlamydial NGU (11.1%) did not differ significantly from that in men without urethritis (13.5%). Conclusion: Our results showed a significant association between U. urealyticum (biovar 2) and NGU. They also suggest that the presence of U. parvum (biovar 1) in the male urethra might be the result of colonization.

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泌尿器科周術期における抗菌薬の使用方法についてのアンケート報告

山本新吾, 竹山康, 塚本泰司, 清田浩, 小野寺昭一, 頴川晋, 門田晃一, 公文裕巳, 江頭稔久, 内藤誠二, 田中一志, 金丸聰淳, 荒川創一, 守殿貞夫, 安田満, 石原哲, 出口隆, 山田陽司, 村谷哲郎, 松本哲朗, 小川修, 石川清仁, 星長清隆, 岩村正嗣, 斉藤威文, 馬場志郎, 松川雅則

泌尿器科紀要 50 779 - 786 2004.01

非淋菌性尿道炎難治例におけるマイコプラズマの関与

前田真一, 久保田恵章, 玉木正義, 安田満, 出口隆, 吉田隆史, 石古博昭

日本性感染症学会誌 15 139 - 143 2004.01

血清CA19とCAEが異常高値を示した尿管癌の1例

加藤成一, 安田満, 仲野正博, 江原英俊, 石原哲, 出口隆, 坂田佳子, 森, 秀樹, 下川邦泰

泌尿器科紀要 50 99 - 102 2004.01

マイコプラズマ

出口隆, 安田満, 前田真一

性感染症 STD 160 - 168 2004.01

PP3-217 腹腔鏡手術の開腹移行例の検討(一般演題(ポスター))

高橋義人, 横井繁明, 安田満, 亀井信吾, 伊藤慎一, 江原英俊, 水谷晃輔, 中根慶太, 加藤卓, 宇野裕巳, 玉木正義, 前田真一, 出口隆