Updated on 2025/08/22

写真a

 
FUSAGAWA Hiroyori
 
Organization
School of Medicine Department of Physiology Assistant Professor
Title
Assistant Professor
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Research Interests

  • skeletal muscle

  • Gait analysis

  • Sports medicine

  • Pediatric Orthopaedics

Research History

  • Sapporo Medical University   Department of Cellular Physiology and Signal Transduction   Adjunct Assistant Professor

    2022.4

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    Country:Japan

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  • Sapporo Medical University   Department of Orthopedic Surgery

    2015.4 - 2022.3

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  • Uji Tokushukai Hospital   resident

    2013.4 - 2015.3

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Papers

  • Effects of contraction frequency during high-intensity training on fatigue resistance and aerobic adaptations in mouse skeletal muscle. International journal

    Azuma Naito, Tatsuya Sato, Iori Kimura, Nao Tokuda, Nao Yamauchi, Hiroyori Fusagawa, Takashi Yamada

    Journal of applied physiology (Bethesda, Md. : 1985)   2024.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    In high-intensity and sprint interval training, the frequency of contractions is typically higher compared to moderate-intensity continuous training, but it remains unclear whether this contributes to the effective increase in fatigue resistance mechanisms. Here, we investigated the role of contraction frequency in high-intensity training on endurance adaptations of mouse skeletal muscle. Male C57BL/6 mice were divided into groups based on high (0.25 s contraction every 0.5 s) and low (0.25 s contraction every 4.5 s) contraction frequencies, with either 360 contractions per session (Hi360 and Lo360) or 30 contractions per session (Hi30 and Lo30). The plantar flexor muscles were stimulated using in vivo supramaximal electrical stimulation, where all muscle fibers were maximally activated, every other day for 5 weeks. In both the Hi360 and Lo360 groups, where force production declined to less than 40% of the initial value during the training session, muscle endurance, as well as mitochondrial content and respiratory capacity, were increased to a similar extent. In contrast, the rate of torque decline during the training session was more pronounced in the Hi30 group compared to the Lo30 group. In response, the Hi30 group, but not the Lo30 group, exhibited increased fatigue resistance and mitochondrial respiration, which was accompanied by increased PGC-1α expression and an activation of AMPK/Ulk1 pathway. These data suggest that the frequency of contractions is a critical factor in determining the efficient enhancement of mitochondrial respiratory capacity and muscle endurance through high-intensity training, presumably due to promotion of mitochondrial quality control.

    DOI: 10.1152/japplphysiol.00530.2024

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  • Platelet‐rich plasma does not accelerate the healing of damaged muscle following muscle strain

    Hiroyori Fusagawa, Takashi Yamada, Tatsuya Sato, Yuki Ashida, Atsushi Teramoto, Hiroyuki Takashima, Azuma Naito, Nao Tokuda, Nao Yamauchi, Nobutoshi Ichise, Izaya Ogon, Toshihiko Yamashita, Noritsugu Tohse

    Journal of Orthopaedic Research   42 ( 6 )   1190 - 1199   2024.1

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    Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    Abstract

    Although platelet‐rich plasma (PRP) has been widely used regardless of the severity of muscle strain, there have been very few basic studies in which its effects on muscle injury were examined by using models that accurately mimic the clinical muscle strain injury process. Therefore, the aim of this study was to confirm by physiological and structural analyses whether PRP purified by a general preparation method has a muscle healing effect on muscle damage caused by eccentric contraction (ECC). Male Wistar rats were subjected to muscle injury induced by ECC in bilateral plantar flexor muscles using electrical stimulation and an automatically dorsiflexing footplate. The rats were randomly assigned to three groups by type of injection: phosphate‐buffered saline (PBS), leukocyte‐poor PRP (LP‐PRP), or leukocyte‐rich PRP (LR‐PRP) injection into gastrocnemius muscles three times at weekly intervals. The platelet concentrations of the LP‐PRP and LR‐PRP were three to five times higher than that of whole blood. The recovery process of torque strength in the plantar flexor muscle, signal changes in MRI images, and histological evaluation 3 weeks after injury showed no obvious differences among the three groups, and every muscle recovered well from the injury without marked fibrosis. The results that neither LP‐PRP nor LR‐PRP was found to accelerate healing of muscle injuries suggested that conventional preparation and use of PRP for simple muscle injuries caused by muscle strain should be carefully considered, and further basic research using models that accurately mimic clinical practice should be carried out to determine the optimal use of PRP.

    DOI: 10.1002/jor.25784

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  • High-intensity interval training using electrical stimulation ameliorates muscle fatigue in chronic kidney disease-related cachexia by restoring mitochondrial respiratory dysfunction. Reviewed International journal

    Hiroyori Fusagawa, Tatsuya Sato, Takashi Yamada, Azuma Naito, Nao Tokuda, Nao Yamauchi, Nobutoshi Ichise, Toshifumi Ogawa, Takuro Karaushi, Atsushi Teramoto, Noritsugu Tohse

    Frontiers in physiology   15   1423504 - 1423504   2024

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    Language:English   Publishing type:Research paper (scientific journal)  

    BACKGROUND: Exercise, especially high-intensity interval training (HIIT), can increase mitochondrial respiratory capacity and enhance muscular endurance, but its systemic burden makes it difficult to safely and continuously prescribe for patients with chronic kidney disease (CKD)-related cachexia who are in poor general condition. In this study, we examined whether HIIT using electrical stimulation (ES), which does not require whole-body exercise, improves muscle endurance in the skeletal muscle of 5/6 nephrectomized rats, a widely used animal model for CKD-related cachexia. METHODS: Male Wistar rats (10 weeks old) were randomly assigned to a group of sham-operated (Sham) rats and a group of 5/6 nephrectomy (Nx) rats. HIIT was performed on plantar flexor muscles in vivo with supramaximal ES every other day for 4 weeks to assess muscle endurance, myosin heavy-chain isoforms, and mitochondrial respiratory function in Nx rats. A single session was also performed to identify upstream signaling pathways altered by HIIT using ES. RESULTS: In the non-trained plantar flexor muscles from Nx rats, the muscle endurance was significantly lower than that in plantar flexor muscles from Sham rats. The proportion of myosin heavy chain IIa/x, mitochondrial content, mitochondrial respiratory capacity, and formation of mitochondrial respiratory supercomplexes in the plantaris muscle were also significantly decreased in the non-trained plantar flexor muscles from Nx rats than compared to those in plantar flexor muscles from Sham rats. Treatment with HIIT using ES for Nx rats significantly improved these molecular and functional changes to the same degrees as those in Sham rats. Furthermore, a single session of HIIT with ES significantly increased the phosphorylation levels of AMP-activated protein kinase (AMPK) and p38 mitogen-activated protein kinase (MAPK), pathways that are essential for mitochondrial activation signaling by exercise, in the plantar muscles of both Nx and Sham rats. CONCLUSION: The findings suggest that HIIT using ES ameliorates muscle fatigue in Nx rats via restoration of mitochondrial respiratory dysfunction with activation of AMPK and p38 MAPK signaling. Our ES-based HIIT protocol can be performed without placing a burden on the whole body and be a promising intervention that is implemented even in conditions of reduced general performance status such as CKD-related cachexia.

    DOI: 10.3389/fphys.2024.1423504

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  • Skeletal muscle endurance declines with impaired mitochondrial respiration and inadequate supply of acetyl-CoA during muscle fatigue in 5/6 nephrectomized rats

    Hiroyori Fusagawa, Tatsuya Sato, Takashi Yamada, Yuki Ashida, Iori Kimura, Azuma Naito, Nao Tokuda, Nao Yamauchi, Nobutoshi Ichise, Yoshinori Terashima, Izaya Ogon, Atsushi Teramoto, Toshihiko Yamashita, Noritsugu Tohse

    Journal of Applied Physiology   135 ( 4 )   731 - 746   2023.10

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    Publishing type:Research paper (scientific journal)   Publisher:American Physiological Society  

    Mitochondrial dysfunction is associated with decreased skeletal muscle endurance in chronic kidney disease (CKD), but the muscle physiological phenotype and major changes in intramuscular metabolites during muscle fatigue in CKD-related cachexia remain unclear. By using a 5/6 nephrectomized CKD rat model, the present study revealed that CKD is associated with reduced tetanic force in response to repetitive stimuli in a subacute phase, impaired mitochondrial respiration, and inadequate supply of acetyl-CoA during muscle fatigue.

    DOI: 10.1152/japplphysiol.00226.2023

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  • High-intensity interval training using electrical stimulation improves mitochondrial dysfunction and muscle endurance in rats with chronic kidney disease

    Hiroyori Fusagawa, Takashi Yamada, Tatsuya Sato, Yuki Ashida, Asuma Naito, Nao Tokuda, Nao Yamauchi, Nobutoshi Ichise, Takuro Karaushi, Atsushi Teramoto, Toshihiko Yamashita, Noritsugu Tohse

    Biophysical Journal   122 ( 3 )   114a - 114a   2023.2

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    Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.bpj.2022.11.793

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  • Ultrastructural Assessment and Proteomic Analysis in Myofibrillogenesis in the Heart Primordium After Heartbeat Initiation in Rats

    Nobutoshi Ichise, Tatsuya Sato, Hiroyori Fusagawa, Hiroya Yamazaki, Taiki Kudo, Izaya Ogon, Noritsugu Tohse

    Frontiers in Physiology   13   2022.5

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    Publishing type:Research paper (scientific journal)   Publisher:Frontiers Media SA  

    Myofibrillogenesis is an essential process for cardiogenesis and is closely related to excitation-contraction coupling and the maintenance of heartbeat. It remains unclear whether the formation of myofibrils and sarcomeres is associated with heartbeat initiation in the early embryonic heart development. Here, we investigated the association between the ultrastructure of myofibrils assessed by transmission electron microscopy and their proteomic profiling assessed by data-independent acquisition mass spectrometry (DIA-MS) in the rat heart primordia before and after heartbeat initiation at embryonic day 10.0, when heartbeat begins in rats, and in the primitive heart tube at embryonic day 11.0. Bundles of myofilaments were scattered in a few cells of the heart primordium after heartbeat initiation, whereas there were no typical sarcomeres in the heart primordia both before and after heartbeat initiation. Sarcomeres with Z-lines were identified in cells of the primitive heart tube, though myofilaments were not aligned. DIA-MS proteome analysis revealed that only 43 proteins were significantly upregulated by more than 2.0 fold among a total of 7,762 detected proteins in the heart primordium after heartbeat initiation compared with that before heartbeat initiation. Indeed, of those upregulated proteins, 12 (27.9%) were constituent proteins of myofibrils and 10 (23.3%) were proteins that were accessories and regulators for myofibrillogenesis, suggesting that upregulated proteins that are associated with heartbeat initiation were enriched in myofibrillogenesis. Collectively, our results suggest that the establishment of heartbeat is induced by development of bundles of myofilaments with upregulated proteins associated with myofibrillogensis, whereas sarcomeres are not required for the initial heartbeat.

    DOI: 10.3389/fphys.2022.907924

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  • Gait profile score and gait variable scores in spina bifida

    Hiroyori Fusagawa, Hiroki Fujita, Toshikatsu Matsuyama, Nobuaki Himuro, Atsushi Teramoto, Toshihiko Yamashita, Paulo Selber

    Journal of Pediatric Orthopaedics B   31 ( 2 )   e251 - e257   2022.3

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    Publishing type:Research paper (scientific journal)   Publisher:Ovid Technologies (Wolters Kluwer Health)  

    DOI: 10.1097/bpb.0000000000000877

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  • Enhanced glucose metabolism through activation of HIF-1α covers the energy demand in a rat embryonic heart primordium after heartbeat initiation

    Tatsuya Sato, Nobutoshi Ichise, Takeshi Kobayashi, Hiroyori Fusagawa, Hiroya Yamazaki, Taiki Kudo, Noritsugu Tohse

    Scientific Reports   12 ( 1 )   2022.1

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    Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    Abstract

    The initiation of heartbeat is an essential step in cardiogenesis in the heart primordium, but it remains unclear how intracellular metabolism responds to increased energy demands after heartbeat initiation. In this study, embryos in Wistar rats at embryonic day 10, at which heartbeat begins in rats, were divided into two groups by the heart primordium before and after heartbeat initiation and their metabolic characteristics were assessed. Metabolome analysis revealed that increased levels of ATP, a main product of glucose catabolism, and reduced glutathione, a by-product of the pentose phosphate pathway, were the major determinants in the heart primordium after heartbeat initiation. Glycolytic capacity and ATP synthesis-linked mitochondrial respiration were significantly increased, but subunits in complexes of mitochondrial oxidative phosphorylation were not upregulated in the heart primordium after heartbeat initiation. Hypoxia-inducible factor (HIF)-1α was activated and a glucose transporter and rate-limiting enzymes of the glycolytic and pentose phosphate pathways, which are HIF-1α-downstream targets, were upregulated in the heart primordium after heartbeat initiation. These results suggest that the HIF-1α-mediated enhancement of glycolysis with activation of the pentose phosphate pathway, potentially leading to antioxidant defense and nucleotide biosynthesis, covers the increased energy demand in the beating and developing heart primordium.

    Other Link: https://www.nature.com/articles/s41598-021-03832-5

    DOI: 10.1038/s41598-021-03832-5

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  • Larger improvements in fatigue resistance and mitochondrial function with high‐ than with low‐intensity contractions during interval training of mouse skeletal muscle

    Takashi Yamada, Iori Kimura, Yuki Ashida, Katsuyuki Tamai, Hiroyori Fusagawa, Noritsugu Tohse, Håkan Westerblad, Daniel C. Andersson, Tatsuya Sato

    The FASEB Journal   35 ( 11 )   2021.10

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    Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1096/fj.202101204R

    DOI: 10.1096/fj.202101204r

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  • Motion analysis and surgical results of anterior transfer of flexor hallucis longus for equinovarus gait in children with hemiplegia

    Hiroki Fujita, Hiroyori Fusagawa, Hisato Nishibu, Toshiya Nosaka, Toshikatsu Matsuyama, Kousuke Iba, Toshihiko Yamashita

    Journal of Orthopaedic Science   26 ( 3 )   441 - 447   2021.5

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    DOI: 10.1016/j.jos.2020.05.001

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MISC

  • 二分脊椎症児の足部変形に影響を与える歩容の非対称性

    房川 祐頼, 藤田 裕樹, 西部 寿人, 野坂 利也, 松山 敏勝, 樋室 伸顕, 山下 敏彦

    日本整形外科学会雑誌   94 ( 3 )   S954 - S954   2020.3

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    Language:Japanese   Publisher:(公社)日本整形外科学会  

    Ichushi

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  • 二分脊椎症患児の足部変形に影響を与える歩容の非対称性

    房川 祐頼, 藤田 裕樹, 西部 寿人, 野坂 利也, 松山 敏勝, 樋室 伸顕

    日本足の外科学会雑誌   40 ( Suppl. )   S321 - S321   2019.7

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    Language:Japanese   Publisher:(一社)日本足の外科学会  

    Ichushi

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  • 変形治癒に対して骨切り術を施行した2例

    房川 祐頼, 鈴木 智之, 加谷 光規, 渡邉 耕太, 寺本 篤史, 舘田 健児, 木井 雄一郎, 岡田 葉平, 山下 敏彦, 名越 智

    北海道整形災害外科学会雑誌   58 ( 2 )   308 - 308   2017.3

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    Language:Japanese   Publisher:北海道整形災害外科学会  

    Ichushi

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  • 内側楔状開大型高位脛骨骨切り術の患者立脚評価に影響を与える因子の検討

    房川 祐頼, 鈴木 智之, 名越 智, 渡邉 耕太, 寺本 篤史, 舘田 健児, 小助川 維摩, 興村 慎一郎, 木井 雄一郎, 山下 敏彦

    日本整形外科学会雑誌   91 ( 2 )   S583 - S583   2017.3

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    Ichushi

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  • 内側楔状開大型高位脛骨骨切り術の臨床成績に影響を与える因子の検討

    房川 祐頼, 鈴木 智之, 加谷 光規, 渡邉 耕太, 寺本 篤史, 舘田 健児, 木井 雄一郎, 岡田 葉平, 山下 敏彦

    JOSKAS   41 ( 4 )   201 - 201   2016.7

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    Language:Japanese   Publisher:(一社)日本関節鏡・膝・スポーツ整形外科学会  

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Awards

  • Best Poster award

    2018.12   The Japanese Pediatric Orthopaedic Association  

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Research Projects

  • Establishment of new treatment method for dedifferentiated chondrosarcoma

    Grant number:24K12334  2024.4 - 2027.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant amount:\4550000 ( Direct Cost: \3500000 、 Indirect Cost:\1050000 )

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  • 腎不全サルコペニアの運動時エネルギー代謝障害の解明と神経-筋電気刺激療法の確立

    Grant number:23K10505  2023.4 - 2026.3

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    房川 祐頼, 佐藤 達也, 山田 崇史

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    Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )

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  • Roles of mitochondrial function in temperature intervention during recovery from muscle fatigue

    Grant number:20K18069  2020.4 - 2023.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Early-Career Scientists

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    Grant amount:\4160000 ( Direct Cost: \3200000 、 Indirect Cost:\960000 )

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