SAITOU Tsuyoshi

写真a

Affiliation

School of Medicine, Department of Obstetrics and Gynecology

Job title

Professor
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Education 【 display / non-display 】

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    1990

    Sapporo Medical University  

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    1990

    Sapporo Medical University  

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    1986

    Sapporo Medical University  

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    1986

    Sapporo Medical University  

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Degree 【 display / non-display 】

  • Ph. D.

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Professional Memberships 【 display / non-display 】

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    The Japanese Society of Clinical Cytology

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    日本不妊学会

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    The Japan Society of Fertility and Sterility

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    The International Gynecologic Cancer Society

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    Japan Society of Obstetrics and Gynecology

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Research Areas 【 display / non-display 】

  • Life sciences   Obstetrics and gynecology  

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Affiliation 【 display / non-display 】

  • Sapporo Medical University   School of Medicine, Dept.of Obstetrics and Gynecology   Professor  

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Research Interests 【 display / non-display 】

  • 婦人科腫瘍 婦人科手術

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Papers 【 display / non-display 】

  • Identification and phenotypic characterization of neoantigen-specific cytotoxic CD4+ T cells in endometrial cancer

    Minami Fusagawa, Serina Tokita, Kenji Murata, Tasuku Mariya, Mina Umemoto, Shintaro Sugita, Kazuhiko Matsuo, Yoshihiko Hirohashi, Tsuyoshi Saito, Takayuki Kanaseki, Toshihiko Torigoe

    Cancer Immunology Research ( American Association for Cancer Research (AACR) )   2024.12

    　View Summary

    Abstract Tumor-reactive CD4+ T cells often accumulate in the tumor microenvironment (TME) in human cancer, but their functions and roles in antitumor responses remain elusive. Here, we investigated the immunopeptidome of HLA class II–positive (HLA-II+) endometrial cancer with an inflamed TME using a proteogenomic approach. We identified HLA-II neoantigens, one of which induced polyclonal CD4+ tumor-infiltrating lymphocyte (TIL) responses. We then experimentally demonstrated that neoantigen-specific CD4+ TILs lyse target cells in an HLA-II–dependent manner. Single cell transcriptomic analysis of the TME coupled with T-cell receptor (TCR) sequencing revealed the presence of CD4+ T-cell clusters characterized by CXCL13 expression. The CXCL13+ clusters contained two subclusters with distinct cytotoxic gene expression patterns. The identified neoantigen-specific CD4+ T cells were found exclusively in one of the CXCL13+ subclusters characterized by granzyme B and CCL5 expression. These results demonstrate the involvement of tumor-reactive CD4+ T cells with cytotoxic function in immune surveillance of endometrial cancer and reveal their transcriptomic signature.

    DOI

  • Impact of colposcopy-guided carbon dioxide laser vaporization therapy on peripheral cervical intraepithelial neoplasia lesions.

    Tasuku Mariya, Akira Nishikawa, Mina Umemoto, Shiori Ogawa, Tsuyoshi Saito

    Taiwanese journal of obstetrics & gynecology   63 ( 6 ) 846 - 852  2024.11  [International journal]

    　View Summary

    OBJECTIVE: Laser vaporization is less invasive than conization for cervical intraepithelial neoplasia (CIN). The outcome of laser vaporization for CIN is empirically known to depend on the colposcopic findings, especially localization of the lesion. In this study, we sought to identify factors involved in the outcome of laser vaporization. MATERIALS AND METHODS: We retrospectively investigated 290 cases of CIN (CIN2, n = 180; CIN3, n = 110) treated with laser evaporation at Nishikawa Women's Health Clinic between 2018 and 2021. All treatments were performed using a carbon dioxide laser under either colposcopic vision (n = 172) or direct vision using a vaginal speculum (n = 118). Risk factors were statistically examined for cure rate after treatment. RESULTS: Multivariate analysis using a logistic regression model identified independent factors affecting the success of treatment to be high-risk human papillomavirus infection status preoperatively, CIN grade, presence of CIN lesions at the periphery of the cervix, and the surgical method used. Colposcopy-guided laser vaporization reduced the risk of treatment failure by 84% (odds ratio 0.16, 95% confidence interval 0.06-0.46; p = 0.001) compared with direct vision using a vaginal speculum. For lesions at the periphery of the cervix, most of the treatment failures were in the group that was not guided by colposcopy (p = 0.031). CONCLUSION: The presence of a peripheral CIN lesion was suggested to be a risk factor for treatment failure. Laser vaporization under colposcopic vision is recommended for treatment of peripheral CIN lesions.

    DOI PubMed

  • Human papillomavirus self-sampling and urine-sampling tests and the management and short-term outcomes of cervical intraepithelial neoplasia: A prospective observational study.

    Motoki Matsuura, Masato Tamate, Sachiko Nagao, Taishi Akimoto, Fukiko Kasuga, Kimihito Saito, Satoshi Shikanai, Yoko Nishimura, Mizue Teramoto, Tsuyoshi Saito

    The journal of obstetrics and gynaecology research   50 ( 10 ) 1801 - 1807  2024.10  [International journal]

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    AIM: The importance of human papillomavirus (HPV) co-testing using physician-, self-, and urine-collected samples to predict cervical intraepithelial neoplasia (CIN) grade 1-2 prognoses has not been previously reported. Therefore, this study aimed to investigate outcomes of patients with CIN 1-2 who simultaneously underwent physician-, self-, and urine-collection sampling tests. METHODS: This study was conducted in Japan between October 2019 and November 2022 and examined the proportion of cases with CIN 1-2 progressions, the percentage of cases with persistent CIN 1-2, and the outcome differences according to the results of physician-, self-, and urine-sampling tests. RESULTS: There were 105 and 59 CIN 1 and 2 cases, respectively, with progression or persistence in 27 (29.3%) and 21 (50.0%) cases, respectively. The median follow-up was 20 and 12 months, respectively. Progression and persistence of CIN 1 were significantly associated with HPV-positive physician- and self-collected samples. No significant difference was observed between cases with CIN 2 who had HPV-positive and HPV-negative results using any sampling method. CONCLUSIONS: Physician- and self-testing for HPV are crucial for predicting disease progression risk in CIN 1 cases. Future research with an extended observation period and consideration of the progression risks is warranted.

    DOI PubMed

  • Successful Outcome of Stage IB3 Cervical Cancer Treated With Neoadjuvant Chemotherapy Followed by Vaginal Radical Trachelectomy: A Case Report.

    Masato Tamate, Motoki Matsuura, Masayuki Someya, Tasuku Mariya, Shinichi Ishioka, Tsuyoshi Saito

    Cureus   16 ( 10 ) e71626  2024.10  [International journal]

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    Vaginal trachelectomy, which involves resecting the cervix and its parametrium, is a fertility-sparing option for the treatment of early-stage cervical cancer. Although no consensus has been reached on whether simple or radical trachelectomy is preferable, the vaginal approach is typically avoided for tumors larger than 2 cm due to concerns about recurrence. However, some evidence suggests that fertility preservation may still be viable for select patients with larger tumors. This case report describes a woman with bulky cervical cancer who wished to preserve her fertility. After undergoing neoadjuvant chemotherapy (NAC) and vaginal radical trachelectomy (VRT), she achieved a favorable oncological and perinatal outcome, successfully giving birth to a near-full-term baby. The report outlines the patient's management before, during, and after the procedure, including perinatal care. While careful selection of candidates is crucial, accumulating case reports and future trials are expected to shed more light on this treatment approach.

    DOI PubMed

  • Risk-reducing salpingo-oophorectomy for hereditary breast and ovarian cancer patients with vaginal natural orifice transluminal endoscopic surgery (vNOTES).

    Masato Tamate, Motoki Matsuura, Sachiko Nagao, Shoko Kurokawa, Taishi Akimoto, Tsuyoshi Saito

    International journal of gynecological cancer : official journal of the International Gynecological Cancer Society    2024.09  [International journal]

    DOI PubMed

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Misc 【 display / non-display 】

  • LBC検体によるNGSを子宮体癌早期スクリーニングにどう応用するべきか

    松浦基樹, 玉手雅人, 秋元太志, 幅田周太朗, 岩崎雅宏, 畠山晴良, 高根希世子, 山口貴世志, 古川洋一, 齋藤豪

    日本臨床細胞学会雑誌(Web)   62  2023

    J-GLOBAL

  • ヒト子宮内膜症のin vitroモデルを用いた新規病態メカニズムの解明

    長尾来夢, 長尾来夢, 幸野貴之, 金野匠, 倉ありさ, 倉ありさ, 斉藤公仁, 嶋田浩志, 齋藤豪, 小島隆

    日本臨床分子形態学会総会・学術集会講演プログラム・要旨集   55th  2023

    J-GLOBAL

  • 血中エクソソーム由来miRNAを用いた子宮頸癌の治療効果予測

    染谷 正則, 土屋 高旭, 福島 悠希, 長谷川 智一, 井戸川 雅史, 松浦 基樹, 岩崎 雅宏, 廣橋 良彦, 鳥越 俊彦, 齋藤 豪, 坂田 耕一

    日本癌治療学会学術集会抄録集 ( (一社)日本癌治療学会 )  60回   P102 - 2  2022.10

  • 当科における分娩時hysterectomy症例の検討

    石岡 伸一, 染谷 真行, 柏木 葉月, 藤部 佑哉, 真里谷 奨, 馬場 剛, 斎藤 豪

    日本周産期・新生児医学会雑誌 ( (一社)日本周産期・新生児医学会 )  58 ( Suppl.1 ) 297 - 297  2022.06

  • 異常浸潤性胎盤における胎盤絨毛の血管構造の病理組織学的解析

    柏木 葉月, 真里谷 奨, 石岡 伸一, 齋藤 豪

    日本周産期・新生児医学会雑誌 ( (一社)日本周産期・新生児医学会 )  58 ( Suppl.1 ) 363 - 363  2022.06

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Awards 【 display / non-display 】

  • 北海道産婦人科学会、大野学術奨励賞

    2002  

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Research Projects 【 display / non-display 】

  • A study of computer aided diagnosis system of cervical cytology using deep learning.

    Grant-in-Aid for Scientific Research (C)

    Project Year :

    2023.04
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    2026.03
     

    新開 翔太, 鳥越 俊彦, 真里谷 奨, 藤野 雄一, 斉藤 豪

  • Novel vaccination targeting stem cells of cervical cancer

    Grant-in-Aid for Scientific Research (C)

    Project Year :

    2017.04
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    2021.03
     

    Saito Tsuyoshi

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    Cancer stem-like cells (CSCs)/cancer-initiating cells (CICs) are a small subpopulation of cancer cells that are tumorigenic and are resistant to treatments, thus they are focused as treatment targets. We have established sphere-cultured CSCs/CICs from primary human uterine cervical carcinoma, and we isolated several clones from CSCs/CICs in this study. Thus, BORIS sf6 is a cervical CSC/CIC-specific subfamily and has a role in the maintenance of cervical CSCs/CICs. A BORIS C34_24(9)-specific cytotoxic T cell (CTL) clone showed cytotoxicity for BORIS sf6-overexpressing cervical cancer cells. Taken together, the results indicate that the CT antigen BORIS sf6 is specifically expressed in cervical CSCs/CICs, that BORIS sf6 has a role in the maintenance of CSCs/CICs, and that BORIS C34_24(9) peptide is a promising candidate for cervical CSC/CIC-targeting immunotherapy.

  • The role of ASPP2/LSR/AMOT/Merlin/YAP-protein complex at tricellular contacts of normal and cancer cells

    Grant-in-Aid for Scientific Research (C)

    Project Year :

    2016.04
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    2019.03
     

    SATOHISA SEIRO

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    In the present study, we found the role and the regulatory mechanisms of ASPP2/LSR/AMOT/Merlin/YAP-protein complex in the malignancy of cancer cells. In human endometrial cancer tissues, LSR and ASPP2 downregulated during the malignancy. In human endometrial cancer cell line, downregulation of LSR promoted cell migration and cell invasion via transcriptional factor TEAD1/AREG. The mechanisms may be two pathways: 1) the Hippo pathway and 2) YAP/AMOT pathway. Downregulation of ASPP2 decreased LSR, increased phosphorylation of YAP and enhanced cell migration and cell invasion. In normal human epithelial cells derived from the surgery, expression, distribution and regulation of both LSR and ASPP2 were almost similar. Taken together, ASPP2/LSR/AMOT/Merlin/YAP-protein complex are formed at tricellular contacts and they may prevent the malignancy of endometrial cancer.

  • Novel oligomannose liposome-DNA complex DNA vaccination efficiently evokes anti-HPV E6 and E7 CTL responses.

    Grant-in-Aid for Scientific Research (C)

    Project Year :

    2013.04
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    2016.03
     

    Saito Tsuyoshi, Iwasaki Masahiro, Tanaka Ryoichi, Sato Noriyuki

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    The aim of this study was to establish an efficient HPV type 16-targeting cancer immunotherapy. HPV16-derived viral proteins E6 and E7 are expressed in cancerous cells through the progression of the disease and have a role in carcinogenesis but are not expressed in normal cells. In this study, we generated a novel HPV 16 E6 and E7 gene plasmid containing oligomannose liposomes (OML-HPV). We compared the cytotoxic T lymphocyte (CTL) induction efficiency of OML-HPV and that of standard liposome-HPV16 E6 and E7 DNA complex. HPV16 E6-specific CTLs could be generated from HPV 16-positive cervical carcinoma patient's peripheral blood mononuclear cells by stimulating OML-HPV, but could not by stimulating standard liposome-HPV 16 E6, E7 DNA complex. These results indicate that OML-HPV is a more effective approach than DNA vaccination using standard liposomes, and that a novel HLA-A24-restricted peptide, E6 66-74, might be a suitable target of cervical cancer immunotherapy.

  • Assessment of risk factors for ovarian cancer including serum equol concentration by histopathology with a case-control study

    Grant-in-Aid for Scientific Research (B)

    Project Year :

    2010
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    2012
     

    MORI Mitsuru, SAITO Tsuyoshi

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    We conducted a case-control study on association of serum components with ovarian cancer (OC) risk. OC cases (N=71) and their controls (N=80) were recruited from inpatients at Sapporo Medical University Hospital. Unconditional logistic regression analysis was used for adjustment of age, number of love-births, and BMI. As a result from the multivariate analysis, serum daizein level was significantly associated with decreased risk of OC (P

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