SAITOU Tsuyoshi

写真a

Affiliation

School of Medicine, Department of Perinatal Medicine

Job title

Professor
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Education 【 display / non-display 】

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    1990

    Sapporo Medical University  

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    1990

    Sapporo Medical University  

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    1986

    Sapporo Medical University  

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    1986

    Sapporo Medical University  

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Degree 【 display / non-display 】

  • Ph. D.

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Professional Memberships 【 display / non-display 】

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    The Japan Society of Fertility and Sterility

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    日本不妊学会

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    The Japanese Society of Clinical Cytology

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    日本臨床電子顕微鏡学会

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    The International Gynecologic Cancer Society

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Research Areas 【 display / non-display 】

  • Life sciences   Obstetrics and gynecology  

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Affiliation 【 display / non-display 】

  • Sapporo Medical University   School of Medicine, Dept.of Obstetrics and Gynecology   Professor  

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Research Interests 【 display / non-display 】

  • 婦人科腫瘍 婦人科手術

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Papers 【 display / non-display 】

  • Large number of CD68+/CD163+ Hofbauer cells and characteristic vascular structural alterations in the placental villi of cases with placenta accreta spectrum.

    Hazuki Kashiwagi, Kengo Shigehara, Terufumi Kubo, Yoshihiko Hirohashi, Tasuku Mariya, Kazuhiko Matsuo, Tomoyuki Minowa, Shin-Ichi Ishioka, Kenji Murata, Takayuki Kanaseki, Tomohide Tsukahara, Tadashi Hasegawa, Tsuyoshi Saito, Toshihiko Torigoe

    Medical molecular morphology    2025.03  [Domestic journal]

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    Placenta accreta spectrum (PAS) is a serious disease leading to complications and maternal death. The objective of the study was to characterize the placental villi and blood vessels of PAS villi histopathologically. We investigated 10 cases of PAS (five cases of placenta increta, two cases of placenta accreta, and three cases of placenta percreta) histologically. Immunohistochemical staining using anti-CD68 or anti-CD163 antibodies was performed to detect and count Hofbauer cells. Immunohistochemical staining with an anti-CD34 antibody was used to detect vascular endothelial cells, and the number and area of vessels were analyzed. The numbers of CD68-positive or CD163-positive Hofbauer cells were larger in PAS cases compared with control cases. The vascular area in villi was smaller in PAS cases compared with control cases. The number of blood vessels in villi was slightly higher in PAS cases than in control cases. The numbers of Hofbauer cells and vessels in villi were larger in PAS cases compared with control cases, whereas the area of vessels in villi was smaller in PAS cases compared with control cases. Although their biological meaning is elusive, these findings provide novel insights into the pathogenesis of PAS, particularly regarding the role of Hofbauer cells in immune-suppressive role and angiogenesis and the alterations in vascular structure and hemodynamics in the chorionic villi.

    DOI PubMed

  • Maternal age and the rising incidence of hypertensive disorders of pregnancy: A comprehensive analysis of national claims data from Japan.

    Naomi Maeda, Masayuki Koyama, Shintaro Takatsuka, Keisuke Oyatani, Nobuaki Himuro, Tasuku Mariya, Yoshika Kuno, Shiro Hinotsu, Tsuyoshi Saito, Hirofumi Ohnishi

    PloS one   20 ( 2 ) e0319177  2025  [International journal]

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    BACKGROUND: Hypertensive disorders of pregnancy (HDP) significantly increase the risk of developing hypertension and cardiovascular disease (CVD) later in life and are a major cause of maternal mortality. However, little is known about the nationwide, long-term, all-inclusive status of HDP. OBJECTIVE: To estimate the incidence of HDP from 2011 to 2019 in Hokkaido, Japan, with a focus on age groups. METHODS: Using National Database (NDB) insurance medical data, a retrospective analysis was conducted. Due to the absence of direct pregnancy data, birth numbers were used as a surrogate for the number of pregnant women to calculate the incidence of HDP. RESULTS: The average incidence rate of HDP over 9 years was 6.37%. The incidence rate was lowest among women aged 25-29 years, at 5.58% (95% confidence interval [CI]: 5.43-5.73), and increased with age, peaking at 10.58% (95% CI: 10.10-11.09) among women over 40 years. Notably, the incidence rate for women under 20 years of age was 6.70% (95% CI: 5.97-7.51), which was higher than that for women in their 20s. A mean annual increase of 0.25% in age-adjusted incidence was observed during this period, which was statistically significant (R² =  0.87, p <  0.01). CONCLUSION: This study reveals that the risk of developing HDP is associated with both older childbearing and younger pregnancies and follows a J-curve, suggesting that factors other than maternal aging also contribute to the increased incidence of HDP and that further research on risk factors for HDP, which is on the rise worldwide, is urgently needed.

    DOI PubMed

  • Identification and phenotypic characterization of neoantigen-specific cytotoxic CD4+ T cells in endometrial cancer

    Minami Fusagawa, Serina Tokita, Kenji Murata, Tasuku Mariya, Mina Umemoto, Shintaro Sugita, Kazuhiko Matsuo, Yoshihiko Hirohashi, Tsuyoshi Saito, Takayuki Kanaseki, Toshihiko Torigoe

    Cancer Immunology Research ( American Association for Cancer Research (AACR) )   2024.12

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    Abstract Tumor-reactive CD4+ T cells often accumulate in the tumor microenvironment (TME) in human cancer, but their functions and roles in antitumor responses remain elusive. Here, we investigated the immunopeptidome of HLA class II–positive (HLA-II+) endometrial cancer with an inflamed TME using a proteogenomic approach. We identified HLA-II neoantigens, one of which induced polyclonal CD4+ tumor-infiltrating lymphocyte (TIL) responses. We then experimentally demonstrated that neoantigen-specific CD4+ TILs lyse target cells in an HLA-II–dependent manner. Single cell transcriptomic analysis of the TME coupled with T-cell receptor (TCR) sequencing revealed the presence of CD4+ T-cell clusters characterized by CXCL13 expression. The CXCL13+ clusters contained two subclusters with distinct cytotoxic gene expression patterns. The identified neoantigen-specific CD4+ T cells were found exclusively in one of the CXCL13+ subclusters characterized by granzyme B and CCL5 expression. These results demonstrate the involvement of tumor-reactive CD4+ T cells with cytotoxic function in immune surveillance of endometrial cancer and reveal their transcriptomic signature.

    DOI

  • 【エネルギーデバイス アラカルト-知っておきたい使い方の留意点と技術革新】各種デバイスの原理・有用性と使用上の注意点 シーリングデバイス

    松浦 基樹, 玉手 雅人, 齋藤 豪

    臨床婦人科産科 ( (株)医学書院 )  78 ( 12 ) 1104 - 1111  2024.12

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    ＜文献概要＞●シーリングデバイスは直径7mmまでの血管や組織束がシール可能であり,超音波凝固切開装置より太い血管をシールすることができる.●シーリングデバイスは高周波エネルギーを瞬時に供給して血管のシールを行うため,手術効率が向上するだけではなく,熱の拡散を極力抑えることで周囲の組織への影響を最小限に抑えることができる.●外科医は各デバイスの原理や有用性・注意点などを習熟したうえで,これらのデバイスを使用するべきである.

  • 【エネルギーデバイス アラカルト-知っておきたい使い方の留意点と技術革新】総論 モードの特性と使い分け

    玉手 雅人, 松浦 基樹, 齋藤 豪

    臨床婦人科産科 ( (株)医学書院 )  78 ( 12 ) 1083 - 1090  2024.12

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    ＜文献概要＞●電気メスのモードと本体の使い分け:電気メスの効果と性能は,モードと出力設定で変化するが,電気メスを接続する本体の種類によっても異なる.●他の手術機器との使い分け,バイポーラ・アドバンスドバイポーラ・バイクランプの使い分け:電気メス,バイポーラ,アドバンスドバイポーラ,超音波凝固切開装置の組み合わせは術式・手技・コストで考慮する.●機械の特性を理解して,術者が自身の技量や手術スタイルに合わせてエネルギーデバイスを扱うことが重要と思われる.

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Misc 【 display / non-display 】

  • LBC検体によるNGSを子宮体癌早期スクリーニングにどう応用するべきか

    松浦基樹, 玉手雅人, 秋元太志, 幅田周太朗, 岩崎雅宏, 畠山晴良, 高根希世子, 山口貴世志, 古川洋一, 齋藤豪

    日本臨床細胞学会雑誌(Web)   62  2023

    J-GLOBAL

  • ヒト子宮内膜症のin vitroモデルを用いた新規病態メカニズムの解明

    長尾来夢, 長尾来夢, 幸野貴之, 金野匠, 倉ありさ, 倉ありさ, 斉藤公仁, 嶋田浩志, 齋藤豪, 小島隆

    日本臨床分子形態学会総会・学術集会講演プログラム・要旨集   55th  2023

    J-GLOBAL

  • 血中エクソソーム由来miRNAを用いた子宮頸癌の治療効果予測

    染谷 正則, 土屋 高旭, 福島 悠希, 長谷川 智一, 井戸川 雅史, 松浦 基樹, 岩崎 雅宏, 廣橋 良彦, 鳥越 俊彦, 齋藤 豪, 坂田 耕一

    日本癌治療学会学術集会抄録集 ( (一社)日本癌治療学会 )  60回   P102 - 2  2022.10

  • 当科における分娩時hysterectomy症例の検討

    石岡 伸一, 染谷 真行, 柏木 葉月, 藤部 佑哉, 真里谷 奨, 馬場 剛, 斎藤 豪

    日本周産期・新生児医学会雑誌 ( (一社)日本周産期・新生児医学会 )  58 ( Suppl.1 ) 297 - 297  2022.06

  • 異常浸潤性胎盤における胎盤絨毛の血管構造の病理組織学的解析

    柏木 葉月, 真里谷 奨, 石岡 伸一, 齋藤 豪

    日本周産期・新生児医学会雑誌 ( (一社)日本周産期・新生児医学会 )  58 ( Suppl.1 ) 363 - 363  2022.06

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Awards 【 display / non-display 】

  • 北海道産婦人科学会、大野学術奨励賞

    2002  

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Research Projects 【 display / non-display 】

  • A study of computer aided diagnosis system of cervical cytology using deep learning.

    Grant-in-Aid for Scientific Research (C)

    Project Year :

    2023.04
    -
    2026.03
     

    新開 翔太, 鳥越 俊彦, 真里谷 奨, 藤野 雄一, 斉藤 豪

  • Novel vaccination targeting stem cells of cervical cancer

    Grant-in-Aid for Scientific Research (C)

    Project Year :

    2017.04
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    2021.03
     

    Saito Tsuyoshi

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    Cancer stem-like cells (CSCs)/cancer-initiating cells (CICs) are a small subpopulation of cancer cells that are tumorigenic and are resistant to treatments, thus they are focused as treatment targets. We have established sphere-cultured CSCs/CICs from primary human uterine cervical carcinoma, and we isolated several clones from CSCs/CICs in this study. Thus, BORIS sf6 is a cervical CSC/CIC-specific subfamily and has a role in the maintenance of cervical CSCs/CICs. A BORIS C34_24(9)-specific cytotoxic T cell (CTL) clone showed cytotoxicity for BORIS sf6-overexpressing cervical cancer cells. Taken together, the results indicate that the CT antigen BORIS sf6 is specifically expressed in cervical CSCs/CICs, that BORIS sf6 has a role in the maintenance of CSCs/CICs, and that BORIS C34_24(9) peptide is a promising candidate for cervical CSC/CIC-targeting immunotherapy.

  • The role of ASPP2/LSR/AMOT/Merlin/YAP-protein complex at tricellular contacts of normal and cancer cells

    Grant-in-Aid for Scientific Research (C)

    Project Year :

    2016.04
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    2019.03
     

    SATOHISA SEIRO

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    In the present study, we found the role and the regulatory mechanisms of ASPP2/LSR/AMOT/Merlin/YAP-protein complex in the malignancy of cancer cells. In human endometrial cancer tissues, LSR and ASPP2 downregulated during the malignancy. In human endometrial cancer cell line, downregulation of LSR promoted cell migration and cell invasion via transcriptional factor TEAD1/AREG. The mechanisms may be two pathways: 1) the Hippo pathway and 2) YAP/AMOT pathway. Downregulation of ASPP2 decreased LSR, increased phosphorylation of YAP and enhanced cell migration and cell invasion. In normal human epithelial cells derived from the surgery, expression, distribution and regulation of both LSR and ASPP2 were almost similar. Taken together, ASPP2/LSR/AMOT/Merlin/YAP-protein complex are formed at tricellular contacts and they may prevent the malignancy of endometrial cancer.

  • Novel oligomannose liposome-DNA complex DNA vaccination efficiently evokes anti-HPV E6 and E7 CTL responses.

    Grant-in-Aid for Scientific Research (C)

    Project Year :

    2013.04
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    2016.03
     

    Saito Tsuyoshi, Iwasaki Masahiro, Tanaka Ryoichi, Sato Noriyuki

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    The aim of this study was to establish an efficient HPV type 16-targeting cancer immunotherapy. HPV16-derived viral proteins E6 and E7 are expressed in cancerous cells through the progression of the disease and have a role in carcinogenesis but are not expressed in normal cells. In this study, we generated a novel HPV 16 E6 and E7 gene plasmid containing oligomannose liposomes (OML-HPV). We compared the cytotoxic T lymphocyte (CTL) induction efficiency of OML-HPV and that of standard liposome-HPV16 E6 and E7 DNA complex. HPV16 E6-specific CTLs could be generated from HPV 16-positive cervical carcinoma patient's peripheral blood mononuclear cells by stimulating OML-HPV, but could not by stimulating standard liposome-HPV 16 E6, E7 DNA complex. These results indicate that OML-HPV is a more effective approach than DNA vaccination using standard liposomes, and that a novel HLA-A24-restricted peptide, E6 66-74, might be a suitable target of cervical cancer immunotherapy.

  • Assessment of risk factors for ovarian cancer including serum equol concentration by histopathology with a case-control study

    Grant-in-Aid for Scientific Research (B)

    Project Year :

    2010
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    2012
     

    MORI Mitsuru, SAITO Tsuyoshi

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    We conducted a case-control study on association of serum components with ovarian cancer (OC) risk. OC cases (N=71) and their controls (N=80) were recruited from inpatients at Sapporo Medical University Hospital. Unconditional logistic regression analysis was used for adjustment of age, number of love-births, and BMI. As a result from the multivariate analysis, serum daizein level was significantly associated with decreased risk of OC (P

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