SAITOU Tsuyoshi

写真a

Affiliation

School of Medicine, Department of Obstetrics and Gynecology

Job title

Professor

Education 【 display / non-display

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    -
    1990

    Sapporo Medical University  

  •  
    -
    1990

    Sapporo Medical University  

  •  
    -
    1986

    Sapporo Medical University  

  •  
    -
    1986

    Sapporo Medical University  

Degree 【 display / non-display

  • Ph. D.

Professional Memberships 【 display / non-display

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    The Japanese Society of Clinical Cytology

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    日本不妊学会

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    The Japan Society of Fertility and Sterility

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    The International Gynecologic Cancer Society

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    Japan Society of Obstetrics and Gynecology

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Research Areas 【 display / non-display

  • Life sciences   Obstetrics and gynecology  

Affiliation 【 display / non-display

  • Sapporo Medical University   School of Medicine, Dept.of Obstetrics and Gynecology   Professor  

 

Research Interests 【 display / non-display

  • 婦人科腫瘍 婦人科手術

Papers 【 display / non-display

  • The frequency and pathogenicity of BRCA1 and BRCA2 variants in the general Japanese population.

    Masashi Idogawa, Tasuku Mariya, Yumi Tanaka, Tsuyoshi Saito, Hiroshi Nakase, Takashi Tokino, Akihiro Sakurai

    Journal of human genetics    2024.02  [International journal]

     View Summary

    Hereditary breast and ovarian cancer syndrome (HBOC) resulting from pathogenic variants of BRCA1 or BRCA2 is the most common and well-documented hereditary tumor. Although founder variants have been identified in population-based surveys in various countries, the types of variants are not uniform across races and regions. Recently, the Tohoku Medical Megabank Organization (ToMMo) released whole-genome sequence data including approximately 54,000 individuals from the general population of the Tohoku area in Japan. We analyzed these data and comprehensively identified the prevalence of BRCA1/2 pathogenic and truncating variants. We believe that an accurate understanding of the unique distribution and characteristics of pathogenic BRCA1/2 variants in Japan through this analysis will enable better surveillance and intervention for HBOC patients, not only in Japan but also worldwide.

    DOI PubMed

  • Identification of cancer driver mutations in liquid-based cytology samples for the screening of endometrial diseases

    Motoki Matsuura, Kiyoko Takane, Kiyoshi Yamaguchi, Tsuneo Ikenoue, Seira Hatakeyama, Shoko Kurokawa, Masato Tamate, Taishi Akimoto, Masahiro Iwasaki, Shintaro Sugita, Tadashi Hasegawa, Yasunori Ota, Tsuyoshi Saito, Yoichi Furukawa

    BJC Reports ( Springer Science and Business Media LLC )  1 ( 1 )  2023.11

     View Summary

    Abstract Background Endometrial cancer (EC) is one of the leading causes of cancer death among women and early detection is crucial for its successful treatment. We previously showed that cytological examination combined with genetic analysis using liquid-based cytology (LBC) samples improved the diagnostic sensitivity of EC. Methods Among 218 individuals who underwent endometrial screening by LBC, 208 samples were analysed by cancer-panel sequencing. Excluding 13 samples with low sequence coverage, we further analysed 195 samples. Results Among the 195 women analysed, 39 and 15 were eventually shown to suffer from malignant endometrial and non-endometrial neoplasms, respectively. Driver mutation(s) were found in nine of the 15 cytology-negative patients with endometrial malignancy, corroborating that combination of cytological and genetic analyses should increase the sensitivity of the diagnosis of malignant endometrial neoplasms. Importantly, driver alterations were found in 32 of 125 women without malignant or premalignant diseases, which raises caution for the interpretation of genetic alterations detected in the endometrial samples. Comparison between the driver mutations in the 32 subjects and those in the 29 endometrial malignancies unveiled that the number of mutations and mutations in PTEN, CTNNB1, and TP53 may be applied for the assessment and prediction of development of EC. Conclusions The genetic analysis combined with liquid-based cytology is useful to improve the diagnostic sensitivity of endometrial neoplasms. However, caution must be taken when cancer-associated mutation(s) are detected in the genetic analysis. Further investigation may clarify the risk of malignant endometrial neoplasms in women with driver mutation(s).

    DOI

  • Pregnancy-specific beta-1-glycoprotein 6 is a potential novel diagnostic biomarker of placenta accreta spectrum.

    Hazuki Kashiwagi, Tasuku Mariya, Mina Umemoto, Shiori Ogawa, Yoshihiko Hirohashi, Yuya Fujibe, Terufumi Kubo, Masayuki Someya, Tsuyoshi Baba, Shinichi Ishioka, Toshihiko Torigoe, Tsuyoshi Saito

    Medical molecular morphology    2023.10  [Domestic journal]

     View Summary

    Early diagnosis is essential for the safer perinatal management of placenta accreta spectrum (PAS). We used transcriptome analysis to investigate diagnostic maternal serum biomarkers and the mechanisms of PAS development. We analyzed eight formalin-fixed paraffin-embedded placental specimens from two placenta increta and three placenta percreta cases who underwent cesarean hysterectomy at Sapporo Medical University Hospital between 2013 and 2019. Invaded placental regions were isolated from the uterine myometrium and RNA was extracted. The transcriptome difference between normal placenta and PAS was analyzed by microarray analysis. The PAS group showed markedly decreased expression of placenta-specific genes such as LGALS13 and the pregnancy-specific beta-1-glycoprotein (PSG) family. Term enrichment analysis revealed changes in genes related to cellular protein catabolic process, female pregnancy, autophagy, and metabolism of lipids. From the highly dysregulated genes in the PAS group, we investigated the expression of PSG family members, which are secreted into the intervillous space and can be detected in maternal serum from the early stage of pregnancy. The gene expression level of PSG6 in particular was progressively decreased from placenta increta to percreta. The PSG family, especially PSG6, is a potential biomarker for PAS diagnosis.

    DOI PubMed

  • Endocrinological and Metabolic Heterogeneity is low in Japanese Women with Polycystic Ovary Syndrome.

    Tsuyoshi Baba, Toshiaki Endo, Tasuku Mariya, Yoshika Kuno, Hiroyuki Honnma, Mika Kanaya, Tsuyoshi Saito

    Journal of obstetrics and gynaecology Canada : JOGC = Journal d'obstetrique et gynecologie du Canada : JOGC     102217 - 102217  2023.09  [International journal]

     View Summary

    OBJECTIVE: This study aims to evaluate the endocrine differences among polycystic ovary syndrome (PCOS) phenotypes in Japanese women. METHODS: 118 Japanese women that we diagnosed with PCOS agreed to be included in the study. The study group was classified into the following four phenotypes: A) hyperandrogenism (HA); ovulatory disorder (OvD) and polycystic ovary morphology (PCOM); B) HA and OvD; C) HA and PCOM; and D) OvD and PCOM. We also recruited 66 healthy Japanese women to the study as control participants. Age, body mass index, androgens, luteinizing hormone, follicle-stimulating hormone, and insulin resistance index were evaluated and compared. RESULTS: The proportions of phenotypes A, B, C, and D were 57/120 (47.5%), 4/120 (3.3%), 13/120 (10.8%), and 46/120 (38.3%), respectively. The proportion of phenotype B was too small; therefore, phenotypes A and B were grouped as classical PCOS for intergroup comparisons. The luteinizing hormone/follicle-stimulating hormone ratio in the classical PCOS group was higher than that in the phenotype D group (P < 0.001). Androgen concentrations in the phenotype D group were significantly lower than those in the other groups (P < 0.01). Phenotype D was more common in lean women with PCOS. The surrogate marker of insulin resistance (homeostasis model assessment of insulin resistance (HOMA-IR)) was not different irrespective of PCOS and its phenotypes. CONCLUSION: Except for androgens, endocrine differences by PCOS phenotype are not evident, suggesting that diversity among patients with PCOS is relatively low in Japanese women.

    DOI PubMed

  • Laparoscopic retrovesical lower uterine segment bypass for hysterectomy with previous caesarean section: Hung Up the Bladder Bypass (HUBB) technique.

    Masato Tamate, Motoki Matsuura, Nagisa Wada, Takaki Adachi, Kazuma Yorozu, Chihiro Arimoto, Tsuyoshi Saito

    Journal of gynecology obstetrics and human reproduction     102629 - 102629  2023.07  [International journal]

     View Summary

    Patients with a history of cesarean section and a highly adherent bladder may have difficulty in bladder dissection and may suffer damage. It may also be difficult to orient the patient for repair in the event of damage. In such cases, dissection from the side between the bladder and cervix is a good way to avoid complication. This video reviews the steps of the HUBB technique procedure, provides tips and tricks for performing a successful adhesiolysis, and illustrates the procedure's adaptability. The first is the identification of the uterine artery and its position. Step2, a Laparoscopic retrovesical lower uterine segment bypass is created. Step3 a Hung Up the Bladder Bypass is created, the bladder is completely dissected. Step4, an adhesiolysis and leak test is performed. In this report, we would like to introduce a technique for creating a retrovesical lower uterine segment bypass using a laparoscope and traction on the bypass to dissect the bladder.

    DOI PubMed

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Misc 【 display / non-display

  • LBC検体によるNGSを子宮体癌早期スクリーニングにどう応用するべきか

    松浦基樹, 玉手雅人, 秋元太志, 幅田周太朗, 岩崎雅宏, 畠山晴良, 高根希世子, 山口貴世志, 古川洋一, 齋藤豪

    日本臨床細胞学会雑誌(Web)   62  2023

    J-GLOBAL

  • 血中エクソソーム由来miRNAを用いた子宮頸癌の治療効果予測

    染谷 正則, 土屋 高旭, 福島 悠希, 長谷川 智一, 井戸川 雅史, 松浦 基樹, 岩崎 雅宏, 廣橋 良彦, 鳥越 俊彦, 齋藤 豪, 坂田 耕一

    日本癌治療学会学術集会抄録集 ( (一社)日本癌治療学会 )  60回   P102 - 2  2022.10

  • 当科における分娩時hysterectomy症例の検討

    石岡 伸一, 染谷 真行, 柏木 葉月, 藤部 佑哉, 真里谷 奨, 馬場 剛, 斎藤 豪

    日本周産期・新生児医学会雑誌 ( (一社)日本周産期・新生児医学会 )  58 ( Suppl.1 ) 297 - 297  2022.06

  • 異常浸潤性胎盤における胎盤絨毛の血管構造の病理組織学的解析

    柏木 葉月, 真里谷 奨, 石岡 伸一, 齋藤 豪

    日本周産期・新生児医学会雑誌 ( (一社)日本周産期・新生児医学会 )  58 ( Suppl.1 ) 363 - 363  2022.06

  • 当院における思春期前症例の卵巣組織ガラス化保存と周術期の問題点について

    馬場 剛, 玉手 雅人, 春日 芙貴子, 長尾 沙智子, 藤部 佑哉, 木井 菜摘, 君塚 基修, 齋藤 豪

    日本がん・生殖医療学会誌 ( (一社)日本がん・生殖医療学会 )  5 ( 1 ) 139 - 139  2022.01

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Awards 【 display / non-display

  • 北海道産婦人科学会、大野学術奨励賞

    2002  

Research Projects 【 display / non-display

  • A study of computer aided diagnosis system of cervical cytology using deep learning.

    Grant-in-Aid for Scientific Research (C)

    Project Year :

    2023.04
    -
    2026.03
     

    新開 翔太, 鳥越 俊彦, 真里谷 奨, 藤野 雄一, 斉藤 豪

  • Novel vaccination targeting stem cells of cervical cancer

    Grant-in-Aid for Scientific Research (C)

    Project Year :

    2017.04
    -
    2021.03
     

    Saito Tsuyoshi

     View Summary

    Cancer stem-like cells (CSCs)/cancer-initiating cells (CICs) are a small subpopulation of cancer cells that are tumorigenic and are resistant to treatments, thus they are focused as treatment targets. We have established sphere-cultured CSCs/CICs from primary human uterine cervical carcinoma, and we isolated several clones from CSCs/CICs in this study. Thus, BORIS sf6 is a cervical CSC/CIC-specific subfamily and has a role in the maintenance of cervical CSCs/CICs. A BORIS C34_24(9)-specific cytotoxic T cell (CTL) clone showed cytotoxicity for BORIS sf6-overexpressing cervical cancer cells. Taken together, the results indicate that the CT antigen BORIS sf6 is specifically expressed in cervical CSCs/CICs, that BORIS sf6 has a role in the maintenance of CSCs/CICs, and that BORIS C34_24(9) peptide is a promising candidate for cervical CSC/CIC-targeting immunotherapy.

  • The role of ASPP2/LSR/AMOT/Merlin/YAP-protein complex at tricellular contacts of normal and cancer cells

    Grant-in-Aid for Scientific Research (C)

    Project Year :

    2016.04
    -
    2019.03
     

    SATOHISA SEIRO

     View Summary

    In the present study, we found the role and the regulatory mechanisms of ASPP2/LSR/AMOT/Merlin/YAP-protein complex in the malignancy of cancer cells. In human endometrial cancer tissues, LSR and ASPP2 downregulated during the malignancy. In human endometrial cancer cell line, downregulation of LSR promoted cell migration and cell invasion via transcriptional factor TEAD1/AREG. The mechanisms may be two pathways: 1) the Hippo pathway and 2) YAP/AMOT pathway. Downregulation of ASPP2 decreased LSR, increased phosphorylation of YAP and enhanced cell migration and cell invasion. In normal human epithelial cells derived from the surgery, expression, distribution and regulation of both LSR and ASPP2 were almost similar. Taken together, ASPP2/LSR/AMOT/Merlin/YAP-protein complex are formed at tricellular contacts and they may prevent the malignancy of endometrial cancer.

  • Novel oligomannose liposome-DNA complex DNA vaccination efficiently evokes anti-HPV E6 and E7 CTL responses.

    Grant-in-Aid for Scientific Research (C)

    Project Year :

    2013.04
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    2016.03
     

    Saito Tsuyoshi, Iwasaki Masahiro, Tanaka Ryoichi, Sato Noriyuki

     View Summary

    The aim of this study was to establish an efficient HPV type 16-targeting cancer immunotherapy. HPV16-derived viral proteins E6 and E7 are expressed in cancerous cells through the progression of the disease and have a role in carcinogenesis but are not expressed in normal cells. In this study, we generated a novel HPV 16 E6 and E7 gene plasmid containing oligomannose liposomes (OML-HPV). We compared the cytotoxic T lymphocyte (CTL) induction efficiency of OML-HPV and that of standard liposome-HPV16 E6 and E7 DNA complex. HPV16 E6-specific CTLs could be generated from HPV 16-positive cervical carcinoma patient's peripheral blood mononuclear cells by stimulating OML-HPV, but could not by stimulating standard liposome-HPV 16 E6, E7 DNA complex. These results indicate that OML-HPV is a more effective approach than DNA vaccination using standard liposomes, and that a novel HLA-A24-restricted peptide, E6 66-74, might be a suitable target of cervical cancer immunotherapy.

  • Assessment of risk factors for ovarian cancer including serum equol concentration by histopathology with a case-control study

    Grant-in-Aid for Scientific Research (B)

    Project Year :

    2010
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    2012
     

    MORI Mitsuru, SAITO Tsuyoshi

     View Summary

    We conducted a case-control study on association of serum components with ovarian cancer (OC) risk. OC cases (N=71) and their controls (N=80) were recruited from inpatients at Sapporo Medical University Hospital. Unconditional logistic regression analysis was used for adjustment of age, number of love-births, and BMI. As a result from the multivariate analysis, serum daizein level was significantly associated with decreased risk of OC (P

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