Misc - HONMOU Osamu

Pharmacology of Cerebral Ischemia

   2003

中枢性脱髄疾患に対する胚性幹細胞・骨髄の移植.

脳の科学「神経の再生」    2003

Transplantation of neural stem cell into ischemic brain

International Congress Series    2003

脳梗塞への幹細胞移植

分子脳血管病   2   426 - 431  2003

ヒト胎児脳、成人脳、骨髄細胞のラットへの移植

Clin Neurosci   21   1155 - 1157  2003

神経幹細胞移植のソース

脳の科学「神経の再生」    2003

島根医学別冊

  23   73 - 73  2003

胚性幹細胞療法

先端医療シリーズ18・脳神経外科 脳腫瘍の最新医療    2003

神経の再生戦略

Surgery Frontier   10   43 - 48  2003

脳梗塞の幹細胞移植治療

分子脳血管病   2   333 - 337  2003

Transplantation of human neural stem cells into the stroke model

HONMOU Osamu, OKA Shinichi, SASAKI Masanori, TAKAHASHI Akira, TANOOKA Atsushi, HOUKIN Kiyohiro, HASHI Kazuo

Nosotchu   24 ( 3 ) 355 - 358  2002.09

DOI CiNii J-GLOBAL

Remyelination of the spinal cord following intravenous delivery of bone marrow cells

Y Akiyama, C Radtke, O Honmou, JD Kocsis

GLIA ( WILEY-LISS )  39 ( 3 ) 229 - 236  2002.09

　View Summary

Bone marrow contains a population of pluripotent cells that can differentiate into a variety of cell lineages, including neural cells. When injected directly into the demyelinated spinal cord they can elicit remyelination. Recent work has shown that following systemic delivery of bone marrow cells functional improvement occurs in contusive spinal cord injury and stroke models in rat. We report here that secondary to intravenous introduction of an acutely isolated bone marrow cell fraction (mononuclear fraction) from adult rat femoral bones separated on a density gradient, ultrastructurally defined remyelination occurs throughout a focal demyelinated spinal cord lesion. The anatomical pattern of remyelination was characteristic of both oligodendrocyte and Schwann cell myelination; conduction velocity improved in the remyelinated axons. When the injected bone marrow cells were transfected to express LacZ, beta-galactosidase reaction product was observed in some myelin-forming cells in the spinal cord. Intravenous injection of other myelin-forming cells (Schwann cells and olfactory ensheathing cells) or the residual cell fraction of the gradient did not result in remyelination, suggesting that remyelination was specific to the delivery of the mononuclear fraction. While the precise mechanism of the repair, myelination by the bone marrow cells or facilitation of an endogenous repair process, cannot be fully determined, the results demonstrate an unprecedented level of myelin repair by systemic delivery of the mononuclear cells.

DOI

International cooperation to run a brain bank: Austro-German-Japanese (AGJ) brain bank

E Hashimoto, H Ozawa, O Honmou, K Hashi, G Murakami, T Saito, R Riederer

INTERNATIONAL CLINICAL PSYCHOPHARMACOLOGY   17   S137 - S137  2002.08

Research paper, summary (international conference)  

In-111-DTPA cisterno SPECT for the diagnosis of CSF rhinorrhea: Case report

T Yonemori, H Karibe, F Matsuno, O Honmou, Y Minamida, T Uede, S Tanabe, K Hashi

NEUROLOGICAL SURGERY   30 ( 6 ) 617 - 621  2002.06

A quantitative morphometric analysis of rat spinal cord remyelination following transplantation of allogenic Schwann cells

KL Lankford, T Imaizumi, O Honmou, JD Kocsis

JOURNAL OF COMPARATIVE NEUROLOGY ( WILEY-LISS )  443 ( 3 ) 259 - 274  2002.02

　View Summary

Quantitative morphometric techniques were used to assess the extent and pattern of remyelination produced by transplanting allogenic Schwann cells into demyelinated lesions in adult rat spinal cords. The effects of donor age, prior culturing of donor cells, prior lesioning of donor nerves, and host immunosuppression were evaluated by transplanting suspensions of 30,000 acutely dissociated or cultured Schwann cells from neonatal, young adult, or aged adult rat sciatic nerves into X-irradiation and ethidium bromide-induced demyelinated dorsal column lesions, with or without co-transplantation of neonatal optic nerve astrocytes. Three weeks after transplantation, spinal cords were processed for histological analysis. Under all Schwann cell transplant protocols, large areas containing many Schwann cell-like myelinated axon profiles could be readily observed throughout most of the lesion length. Within these "myelin-rich" regions, the vast majority of detectable axons showed a peripheral-like pattern of myelination. However, interaxonal spacing also increased, resulting in densities of myelinated axons that were more similar to peripheral nerve than intact dorsal columns. Freshly isolated Schwann cells remyelinated more axonal length than cultured Schwann cells, and cells from younger donors remyelinated slightly more axon length than cells from older donors, but all Schwann cell transplant protocols remyelinated tens of thousands of millimeters of axon length and remyelinated axons at similar densities. These results indicate that Schwann cells prepared under a variety of conditions are capable of eliciting remyelination, but that the density of remyelinated axons is much lower than the myelinated axon density in intact spinal cords. J. Comp. Neurol. 443:259-274, 2002. (C) 2002 Wiley-Liss, Inc.

DOI

Intravenous administration of autologous bone marrow cells repairs the ischemic lesions in the rat middle cerebral artery occlusion model

Abst. Society for Neuroscience    2002

脳血管障害の再生医療の展望

循環器 New Trendsシリーズ6 脳血管障害-急性期治療から予防まで−    2002

Comparison of remyelination in the remyelination in the focal transplantation and the intravenous administration of autologous bone marrow cells into the rat demyelinated model.

Abst. Society for Neuroscience    2002

光リソグラフィーによるヒト神経幹細胞の遺伝子解析

CI研究    2002

Transplantation of the embryonic stem cells into the ischemic brain

Restorative Neurology and Neuroscience    2002