Misc - HONMOU Osamu

神経幹細胞移植

脳神経外科速報   12   331 - 336  2002

骨髄細胞を用いた神経再生医療

Restorative Neurology and Neuroscience    2002

新専門医によるreview 専門医に求められる最新の知識。神経幹細胞。

井上道夫

脳神経外科速報   12   331 - 336  2002

CiNii

ヒト成熟脳由来クローン化神経幹細胞の移植による末梢性髄鞘の再形成

Clinical Neuroscience   20   79 - 81  2002

神経機能回復へ向けた神経幹細胞移植研究

先端医療シリーズ16・脳神経外科 機能的脳神経外科の最先端    2002

脳血管障害の再生医療の展望

循環器 New Trendsシリーズ「脳血管障害—急性期治療から予防まで」   6   37 - 45  2002

AHA 未破裂脳動脈瘤ガイドライン

脳卒中ナビゲーター, 診断・治療指針    2002

Transplantation of an acutely isolated bone marrow fraction repairs demyelinated adult rat spinal cord axons

M Sasaki, O Honmou, Y Akiyama, T Uede, K Hashi, JD Kocsis

GLIA ( WILEY-LISS )  35 ( 1 ) 26 - 34  2001.07

　View Summary

The potential of bone marrow cells to differentiate into myelin-forming cells and to repair the demyelinated rat spinal cord in vivo was studied using cell transplantation techniques. The dorsal funiculus of the spinal cord was demyelinated by x-irradiation treatment, followed by microinjection of ethidium bromide. Suspensions of a bone marrow cell fraction acutely isolated from femoral bones in LacZ transgenic mice were prepared by centrifugation on a density gradient (Ficoll-Paque) to remove erythrocytes, platelets, and debris. The isolated cell fraction contained hematopoietic and nonhematopoietic stem and precursor cells and lymphocytes. The cells were transplanted into the demyelinated dorsal column lesions of immunosuppressed rats. An intense blue beta -galactosidase reaction was observed in the transplantation zone. The genetically labeled bone marrow cells remyelinated the spinal cord with predominately a peripheral pattern of myelination reminiscent of Schwann cell myelination. Transplantation of CD34(+) hematopoietic stem cells survived in the lesion, but did not form myelin. These results indicate that bone marrow cells can differentiate in vivo into myelin-forming cells and repair demyelinated CNS. GLIA 35:26-34, 2001. (C) 2001 Wiley-Liss, Inc.

DOI

Neural stem cells derived from adult human brain: Implications for a cell therapy for CNS diseases

O Honmou, T Uede, K Hashi

NEUROLOGICAL SURGERY   29 ( 4 ) 293 - 304  2001.04

Transplantation of clonal neural precursor cells derived from adult human brain establishes functional peripheral myelin in the rat spinal cord

Y Akiyama, O Honmou, T Kato, T Uede, K Hashi, JD Kocsis

EXPERIMENTAL NEUROLOGY ( ACADEMIC PRESS INC )  167 ( 1 ) 27 - 39  2001.01

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We examined the myelin repair potential of transplanted neural precursor cells derived from the adult human brain from tissue removed during surgery. Sections of removed brain indicated that nestin-positive cells were found predominately in the subventricular zone around the anterior horns of the lateral ventricle and in the dentate nucleus. Neurospheres were established and the nestin-positive cells were clonally expanded in EGF and bFGF. Upon mitogen withdrawal in vitro, the cells differentiated into neuron- and glia-like cells as distinguished by antigenic profiles; the majority of cells in culture showed neuronal and astrocytic properties with a small number of cells showing properties of oligodendrocytes and Schwann cells. When transplanted into the demyelinated adult rat spinal cord immediately upon mitogen withdrawal, the cells elicited extensive remyelination with a peripheral myelin pattern similar to Schwann cell myelination characterized by large cytoplasmic and nuclear regions, a basement membrane, and PO immunoreactivity. The remyelinated axons conducted impulses at near normal conduction velocities. This suggests that a common neural progenitor cell for CNS and PNS previously described for embryonic neuroepithelial cells may be present in the adult human brain and that transplantation of these cells into the demyelinated spinal cord results in functional remyelination. (C) 2001 Academic Press.

DOI

「細胞治療〜再生医療への展開」神経幹細胞移植

最新医学   12   2666 - 2670  2001

AC133-positive cells derived from bone marrow remyelinate demyelinated axons in the adult rat spinal cord. Abst.

Abst. Society for Neuroscience   0   0-0  2001

Excitatory and inhibitory responses and synaptic transmission differentiated from the adult human neural stem cells in vitro.

Restorative Neurology and Neuroscience.   17   152 - 152  2001

未破裂動脈瘤への対応

本望 修, 上出 廷治, 端 和夫

診断と治療 ( 診断と治療社 )  89 ( 11 ) 2047 - 2052  2001

CiNii

神経疾患の神経幹細胞治療—動物モデルでの試み

脳の科学   23   127 - 132  2001

脳神経疾患と神経再生

脳の科学   23   587 - 590  2001

Mesenchymal stem cells derived from bone marrow remyelinate demyelinated axons in the adult rat spinal cord.

Abst. Society for Neuroscience    2001

神経幹細胞応用の可能性について

脳神経外科   29   293 - 304  2001

T2 reversed fast spin-echo 画像と造影三次元脳表 MR angiography による脳動静脈奇形の画像診断

CI研究   23   165 - 175  2001

Mouse embryonic stem cells remyelinate demyelinated axons in the adult rat spinal cord. Abst.

Abst. Society for Neuroscience    2001