Misc - 一宮　慎吾

G-9 GDNFによる神経芽腫の分化誘導およびRet受容体を介するシグナル伝達の解析(神経芽腫(1))

菱木 知郎, 高橋 英世, 大沼 直躬, 田沼 政裕, 一宮 慎吾, 近藤 和博, 二村 好憲, 尾崎 俊文, 中川原 章

日本小児外科学会雑誌 ( 特定非営利活動法人日本小児外科学会 )  33 ( 3 ) 542 - 542  1997年05月

DOI CiNii

Identification and chromosome assignment of a human gene encoding a novel phosphatidylinositol-3 kinase

Naohiko Seki, Yoshinori Nimura, Miki Ohira, Toshiyuki Saito, Shingo Ichimiya, Nobuo Nomura, Akira Nakagawara

DNA Research   4   355 - 358  1997年01月

　概要を見る

We identified a novel phosphatidylinositol (PI) 3-kinase by screening human brain cDNA libraries with probes designed from the conserved kinase-domain sequence. Analysis of cDNAs indicated that two different forms of transcripts are present: one is the full-length form composed of 1,044 amino acid residues and the other is the short form that the N-terminal 216 amino acid residues including a putative p85 binding domain has been truncated (828 amino acid residues). Database search revealed the sequence of the full-length form to be identical to that recently registered by D. Chantry et al. (Accession No. U86453 in GenBank release, August 1997). Northern blot analysis showed this mRNA to be ubiquitously expressed in various tissues, with relatively higher expression was observed in spleen, thymus and leukocytes. Based on fluorescence in situ hybridization and PCR-based analyses with both human/rodent mono-chromosomal hybrid cell panels and radiation hybrid mapping panels, this gene was localized to chromosome region 1p36.2. This region is frequently lost in a variety of human malignancies, including neuroblastoma. The novel PI3K could be a candidate target of the 1p36 alteration that occurs in neuroendocrine tumors.

DOI PubMed

Veno-occlusive disease of the liver following bone marrow transplantation: A clinical-pathological study of autopsy cases

K Watanabe, H Iwaki, M Satoh, T Ikeda, S Ichimiya, N Suzuki, T Kudoh, T Oda, A Matsuura, M Mori, K Kikuchi

ARTIFICIAL ORGANS ( BLACKWELL SCIENCE INC )  20 ( 10 ) 1145 - 1150  1996年10月

　概要を見る

A total of 28 patients with leukemia underwent intensive therapy with megadose chemotherapy and stem cell transplantation. Of 16 patients who had busulfan therapy, 2 developed the clinical syndrome of veno-occlusive disease of the liver (VOD) characterized by progressive abnormalities in liver function, abdominal pain, and ascites. Postmortem findings showed VOD of the central and sublobular hepatic vein. Immunohistochemical analysis using anti-actin and CD34 mAb was employed in an attempt to clarify the process of fibrosis. Actin positive cells had accumulated around the central vein. Ito cell activation might be responsible for secondary fibrosis following endothelial injury. We report autopsy findings and immunohistochemical results.

Glial cell-derived neurotrophic factor (GDNF) による神経芽細胞腫の分化誘導とc-Retチロシンキナーゼのシグナル伝達について

菱木 知郎, 近藤 和博, 二村 好憲, 一宮 慎吾, 崎山 樹, 中川原 章

神経化学   35 ( 3 ) 494 - 495  1996年09月

CiNii

Thioredoxin dependent peroxidase(TPx)のcDNAクローニングとその生物学的意義

一宮 慎吾, GREENE Mark I, 中川原 章, 崎山 樹

日本分子生物学会年会プログラム・講演要旨集   19   489 - 489  1996年08月

CiNii

IDENTIFICATION OF THE FUNCTIONAL CLASS-I GENES OF THE RAT BY CDNA CLONING

R HONDA, A MATSUURA, S TAKAYAMA, S ICHIMIYA, Y ITOH, K KIKUCHI

TRANSPLANTATION PROCEEDINGS ( APPLETON & LANGE )  27 ( 2 ) 1515 - 1515  1995年04月

A MONOCLONAL-ANTIBODY REACTS WITH T-CELLS AND NOVEL SUBPOPULATION OF RAT BONE-MARROW CELLS

A MATSUURA, M KINEBUCHI, S ICHIMIYA, M SHEN, Y HASHIMOTO, S KATABAMI, K KIKUCHI

TRANSPLANTATION PROCEEDINGS ( APPLETON & LANGE )  27 ( 2 ) 1509 - 1510  1995年04月

STRUCTURAL-ANALYSIS OF THE RAT HOMOLOG OF CD1 - EVIDENCE FOR EVOLUTIONARY CONSERVATION OF THE CD1D CLASS AND WIDESPREAD TRANSCRIPTION BY RAT-CELLS

S ICHIMIYA, K KIKUCHI, A MATSUURA

JOURNAL OF IMMUNOLOGY ( AMER ASSOC IMMUNOLOGISTS )  153 ( 3 ) 1112 - 1123  1994年08月

　概要を見る

The cDNA encoding the rat homologue of CD1 was isolated and the complete nucleotide sequence was determined. It contained an open reading frame of 1008 bp that was capable of encoding a polypeptide with 336 amino acids composed of hydrophobic leader and transmembrane sequences, three extracellular domains, and 5' and 3' untranslated sequences. Comparison of the amino acid sequence of rat CD1 with those of other species revealed that it showed the highest similarity to mouse CD1, which belongs to the CD1D class of the CD1 system and is distinct from the classic CD1 class including CD1a, CD1b, and CD1c expressed primarily on human thymocytes and some dendritic cells. Widespread transcription of rat CD1 was readily detected by Northern blot analysis in nonlymphoid organs, including the liver, kidney, and heart, as well as in lymphoid organs, including the thymus, lymph node, and spleen. Intestinal expression was also demonstrated by the more sensitive reverse transcription-PCR method. Immunoprecipitation with a rabbit anti-rat CD1 Ab showed that rat CD1 was expressed on the cell surface as a beta(2)-microglobulin-associated heterodimer. Southern blot analysis of inbred rat strains suggested that rat CD1 shows limited polymorphism and that only one CD1 gene is detectable in the F344 rat genome. These results provide evidence for the conservation of CD1D class through mammalian evolution and an apparent lack of the classic CD1 class genes in rodents. Functional similarity of rodent CD1 is implied.

STRUCTURAL-ANALYSIS OF THE CD3-ZETA-ETA LOCUS OF THE RAT - EXPRESSION OF ZETA BUT NOT ETA-TRANSCRIPTS BY RAT T-CELLS

Y ITOH, A MATSUURA, M KINEBUCHI, R HONDA, S TAKAYAMA, S ICHIMIYA, S KON, K KIKUCHI

JOURNAL OF IMMUNOLOGY ( AMER ASSOC IMMUNOLOGISTS )  151 ( 9 ) 4705 - 4717  1993年11月

　概要を見る

We analyzed the structure and pattern of expression of rat TCR zeta- and eta-chains to investigate if these components function in activation and development of rat T cells. The rat zeta cDNA contained the complete open reading frame coding for a polypeptide of 164 amino acids and the 5' and 3' noncoding sequences. Comparison of the amino acid sequence to those of mouse and human counterparts revealed a high degree of similarity, more than 85% homology among all three species except for the signal peptide, which was especially high in the cytoplasmic domain including the nucleotide binding site and the possible tyrosine phosphorylation sites. Furthermore, we determined the nucleotide sequences of a rat genomic eta-like sequence located in the 3' region of the rat zeta-gene. Although it showed a high level of nucleotide similarity to mouse and human counterparts, 90.4 and 78.9%, respectively, the deduced polypeptide was very short (only 28 residues) and markedly divergent from the mouse and human eta-specific polypeptides due to frameshift mutations. Transcription of rat zeta was shown to be highly restricted to T cells; abundantly in thymocytes and scarcely in peripheral T cells. Surprisingly, the rat eta transcript could not be detected in any rat tissues so far tested by Northern blot analysis and even by the sensitive reverse transcription-polymerase chain reaction method, whereas it was readily detected in mouse thymus. These findings suggest that the zeta-chain has conserved roles in TCR assembly and TCR-mediated signaling. However, the eta-chain seems not to be indispensable because of its structural diversity among these three species characterized to date and the apparent lack of eta expression in the rat.

MOLECULAR-CLONING OF RAT CLASS-I GENES RELATED TO THE MOUSE TL GENE SUBFAMILY OF THE MAJOR HISTOCOMPATIBILITY COMPLEX

A MATSUURA, S TAKAYAMA, D KOZUTSUMI, S ICHIMIYA, R HONDA, M SHEN, T NATORI, K KIKUCHI

TRANSPLANTATION PROCEEDINGS ( ELSEVIER SCIENCE INC )  25 ( 5 ) 2754 - 2755  1993年10月

ラットCD1抗原 ―組換え蛋白を用いた抗ラットCD1ポリクローナル抗体による同定―

一宮 慎吾, 松浦 晃洋

札幌医学雑誌 = The Sapporo medical journal ( 札幌医科大学 )  62 ( 4 ) 225 - 234  1993年08月

　概要を見る

CD1, the first cluster of differentiation antigens, is a family of cell surface glycoproteins which are noncovalently associated with β2-microglobulin. Although the structure of CD1 is similar to that of major histocompatibility complex class I molecules and cytolytic T cell lines specific for CD1 molecules have been established, which suggest that CD1 antigens may play a role as a presenting molecule, the actual function of CD1 is presently unknown. We have analyzed and identified the rat homologue of CD1 in attempt to confirm the function of CD1 and whether it has been conserved in evolution. The rat CD1 gene was categorized into the CD1D class. In this report, we produced a specific rabbit antiserum to rat CD1 by the prokaryotic expression system and investigated the expression of its antigen. According to FACS and immunohistochemical analyses with the polyclonal antibody, rat CD1 antigen was expressed on the surface of thymocytes and spleen cells, and was detected in hepatic tissue. The expression level of CD1D class in lymphoid organs varied among species. The functional significance of these results is discussed.

DOI CiNii

ESTABLISHMENT OF APOPTOSIS-INDUCING MONOCLONAL-ANTIBODY 2D1 AND 2D1-RESISTANT VARIANTS OF HUMAN T-CELL LINES

S TAKAHASHI, N SATO, S TAKAYAMA, S ICHIMIYA, M SATOH, N HYAKUMACHI, K KIKUCHI

EUROPEAN JOURNAL OF IMMUNOLOGY ( WILEY )  23 ( 8 ) 1935 - 1941  1993年08月

　概要を見る

A monoclonal antibody (mAb), 2D1 (IgM), was identified for its anti-proliferative effect on human T leukemia cell line, SUP-T13. The cells bound with 2D1 showed DNA ladder patterns of oligonucleosomes, demonstrating apoptosis. Peripheral mononuclear cells activated by phytohemagglutinin or OKT3 induced expression of 2D1 antigen and were growth-inhibited by the antibodies. Among the cell lines tested, T cell lines tended to be growth-inhibited by the antibodies. Epstein-Barr virus-transformed B cells were reactive with 2D1, but were not growth-inhibited by the antibodies. We established stable 2D1-resistant variants LAC2D1R and JKT2D1R from the original SUP-T13 and Jurkat T cell lines, respectively.These variant cells demonstrated phenotypes identical to the original cells, including reactivity to 2D1 and expression of cytoplasmic Bc1-2 protein. The 2D1-resistant cells were as sensitive as the original cells to the other apoptosis-inducing stimuli, such as gamma-irradiation or calcium ionophore A23187. However, the 2D1-resistant variants were also insensitive to anti-Fas, another apoptosis-inducing mAb. Binding of 2D1 was blocked by anti-Fas mAb, suggesting that 2D1 reacts with an epitope of human Fas molecules. The present results demonstrate that a 2D1-reactive, but not 2D1-sensitive, population may exist in highly 2D1-sensitive human leukemia T cells and that pairs of 2D1-sensitive and 2D1-resistant cells are useful in the biochemical analysis of Fas-mediated apoptosis in human T cells.

DOI PubMed CiNii