Education 【 display / non-display 】

Education 【 display / non-display 】

• 2015

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  Now

  Sapporo Medical University   医学部   感染制御・臨床検査医学講座  

• 1992

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  2015

  Sapporo Medical University   医学部   泌尿器科学講座  

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  1992

  Sapporo Medical University  

Degree 【 display / non-display 】

Degree 【 display / non-display 】

• 1, Bactericidal effect of levofloxacin on strains with equal susceptibility in an in vitro urinary bladder model. 2, Changes in susceptibility of Pseudomonas aeruginosa to gatifloxacin and carbapenem in an in vitro urinary bladder model.

Professional Memberships 【 display / non-display 】

Professional Memberships 【 display / non-display 】

• 2015

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  Now

  Japanese Society of Laboratory Medicine

• 2015

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  Now

  The Japan Association for Clinical Laboratory Science

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  Japanese Society of Chemotherapy

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  International Union against Sexually Transmitted Infections

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  American Society for Microbiology

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Research Areas 【 display / non-display 】

Research Areas 【 display / non-display 】

• Life sciences   Infectious disease  

Affiliation 【 display / non-display 】

Affiliation 【 display / non-display 】

• Sapporo Medical University   School of Medicine, Department of Infection Control and Laboratory Medicine  

Research Interests 【 display / non-display 】

Research Interests 【 display / non-display 】

• Urinary tract infection, sexually transmitted infection, clinical laboratory medicine

Papers 【 display / non-display 】

Papers 【 display / non-display 】

• Rapid and Integrated Bacterial Evolution Analysis unveils gene mutations and clinical risk of Klebsiella pneumoniae.

  Kojiro Uemura, Toyotaka Sato, Soh Yamamoto, Noriko Ogasawara, Jirachaya Toyting, Kotaro Aoki, Akira Takasawa, Masayuki Koyama, Atsushi Saito, Takayuki Wada, Kaho Okada, Yurie Yoshida, Koji Kuronuma, Chie Nakajima, Yasuhiko Suzuki, Motohiro Horiuchi, Kenichi Takano, Satoshi Takahashi, Hirofumi Chiba, Shin-Ichi Yokota

  Nature communications   16 ( 1 ) 2917 - 2917  2025.03  [International journal]

  　View Summary

  Bacteria continually evolve. Previous studies have evaluated bacterial evolution in retrospect, but this approach is based on only speculation. Cohort studies are reliable but require a long duration. Additionally, identifying which genetic mutations that have emerged during bacterial evolution possess functions of interest to researchers is an exceptionally challenging task. Here, we establish a Rapid and Integrated Bacterial Evolution Analysis (RIBEA) based on serial passaging experiments using hypermutable strains, whole-genome and transposon-directed sequencing, and in vivo evaluations to monitor bacterial evolution in a cohort for one month. RIBEA reveals bacterial factors contributing to serum and antimicrobial resistance by identifying gene mutations that occurred during evolution in the major respiratory pathogen Klebsiella pneumoniae. RIBEA also enables the evaluation of the risk for the progression and the development of invasive ability from the lung to blood and antimicrobial resistance. Our results demonstrate that RIBEA enables the observation of bacterial evolution and the prediction and identification of clinically relevant high-risk bacterial strains, clarifying the associated pathogenicity and the development of antimicrobial resistance at genetic mutation level.

  DOI PubMed

• Traces of pandemic fluoroquinolone-resistant Escherichia coli clone ST131 transmitted from human society to aquatic environments and wildlife in Japan

  Toyotaka Sato, Kojiro Uemura, Mitsuru Yasuda, Aiko Maeda, Toshifumi Minamoto, Kazuki Harada, Michiyo Sugiyama, Shiori Ikushima, Shin-ichi Yokota, Motohiro Horiuchi, Satoshi Takahashi, Testuo Asai

  One Health ( Elsevier BV )  18   100715 - 100715  2024.06  [Refereed]  [International journal]

  　View Summary

  Transmission of antimicrobial-resistant bacteria among humans, animals, and the environment is a growing concern worldwide. The distribution of an international high-risk fluoroquinolone-resistant Escherichia coli clone, ST131, has been documented in clinical settings. However, the transmission of ST131 from humans to surrounding environments remains poorly elucidated. To comprehend the current situation and identify the source of ST131 in nature, we analyzed the genetic features of ST131 isolates from the aquatic environment (lake/river water) and wildlife (fox, raccoon, raccoon dog, and deer) and compared them with the features of isolates from humans in Japan using accessory and core genome single nucleotide polymorphism (SNP) analyses. We identified ST131 isolates belonging to the same phylotype and genome clusters (four of eight clusters were concomitant) with low SNP distance between the human isolates and those from the aquatic environment and wildlife. These findings warn of ST131 transmission between humans and the surrounding environment in Japan.

  DOI PubMed

• JANISでは把握が困難な中小規模病院における緑膿菌の薬剤耐性の状況

  藤谷 好弘, 富樫 篤生, 齋藤 充史, 黒沼 幸治, 高橋 聡

  日本感染症学会総会・学術講演会・日本化学療法学会学術集会合同学会プログラム・抄録集 ( 日本感染症学会・日本化学療法学会 )  98回・72回   np255 - np255  2024.05

• 当院改修時におけるアスペルギルス対策の評価

  黒沼 幸治, 中江 舞美, 韮澤 慎也, 富樫 篤生, 藤谷 好弘, 齋藤 充史, 高橋 聡

  感染症学雑誌 ( (一社)日本感染症学会 )  98 ( 2 ) 281 - 281  2024.03

• 当院改修時におけるアスペルギルス対策の評価

  黒沼 幸治, 中江 舞美, 韮澤 慎也, 富樫 篤生, 藤谷 好弘, 齋藤 充史, 高橋 聡

  感染症学雑誌 ( (一社)日本感染症学会 )  98 ( 2 ) 281 - 281  2024.03

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Misc 【 display / non-display 】

Misc 【 display / non-display 】

• 新型コロナウイルス感染症専用病棟の職員の感染リスクにかかる後向きコホート研究

  藤谷 好弘, 中江 舞美, 中村 広士, 黒沼 幸治, 高橋 聡

  日本環境感染学会総会プログラム・抄録集 ( (一社)日本環境感染学会 )  36回   303 - 303  2021.09

• COVID-19ワクチン接種後感染者1事例の抗体獲得推移

  中江 舞美, 藤谷 好弘, 藤居 賢, 黒沼 幸治, 高橋 聡

  日本環境感染学会総会プログラム・抄録集 ( (一社)日本環境感染学会 )  36回   311 - 311  2021.09

• 鼻腔ぬぐい液および抗原定量検査の臨床的有効性に関する検討

  藤谷 好弘, 黒沼 幸治, 高橋 聡

  感染症学雑誌 ( (一社)日本感染症学会 )  95 ( 臨増 ) 251 - 251  2021.04

• 流産手術後に長期間の反復菌血症を呈しミュンヒハウゼン症候群が疑われた1例

  真里谷 奨, 長井 陽子, 藤谷 好弘, 黒沼 幸治, 高橋 聡

  感染症学雑誌 ( (一社)日本感染症学会 )  95 ( 臨増 ) 288 - 288  2021.04

• 新型コロナウイルス感染症におけるファビピラビル投与の評価

  伊部 裕太, 藤居 賢, 石郷 友之, 國本 雄介, 藤谷 好弘, 黒沼 幸治, 高橋 聡

  日本化学療法学会雑誌 ( (公社)日本化学療法学会 )  69 ( Suppl.A ) 254 - 254  2021.04

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Awards 【 display / non-display 】

Awards 【 display / non-display 】

• 北海道医師会賞・北海道知事賞

  2018.10   北海道医師会・北海道  

• 第98回日本泌尿器科学会 総会賞

  2010.04  

• 第24回稲田賞

  2006   泌尿器科紀要  

• 第365回日本泌尿器科学会北海道地方会 地方会賞

  2005   日本泌尿器科学会北海道地方会  

• 平成14年度日本抗生物質学術協議会・ファイザー感染症研究助成（海外留学助成）

  2002   日本抗生物質学術協議会  

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Research Projects 【 display / non-display 】

Research Projects 【 display / non-display 】

• 淋菌薬剤感受性サーベイランスによる薬剤耐性淋菌に対する有効な抗菌化学療法の検討

  基盤研究(C)

  Project Year :

  2022.04

  -

  2025.03

   

  安田 満, 高橋 聡, 佐藤 勇樹

• Epidemiological and therapeutical study of pathogens associated to non-gonococcal urethritis

  Grant-in-Aid for Scientific Research (B)

  Project Year :

  2013.04

  -

  2016.03

   

  MATSUMOTO TETSURO, HAMASUNA Ryoichi, TAKAHASHI Satoshi, FUKUDA Kazumasa

  　View Summary

  From the specimens of male urethritis, many kinds of microorganisms are detected, but the pathogenicities of microorganisms except for N. gonorrhoeae or C. trachomatis has not been determined. As the new approach, the clone library method was used for analysis of microorganisms of urethritis. This method added quantitative concept to qualitative PCR methods. By this method, the pathogenicities of N. gonorrhoeae adn M. genitalium to the male urethra was confirmed. In addition, it was found that H. influenzae and N. meningitidis would be possible pathogens for male urethritis. By the examination of microorganisms in the oral cavities of patients with male urethritis, it was found that causative bacteria of male urethritis and bacteria of the resident flora in the vagina were detected in the oral cavities of male patients. these indicated that oral sex is closely related to STIs. When we treat male urethritis, we have to eradicate microorganisms in both the urethra and oral cavities.

• Evaluation of stanniocalcin-1 and NADPH oxidase as marker of metastasis in breast cancer

  Grant-in-Aid for Scientific Research (C)

  Project Year :

  2012.04

  -

  2016.03

   

  TANAKA MAKI, TAKAHASHI SATOSHI, KOBAYASHI DAISUKE, WATANABE NAOKI, KURIBAYASHI KAGEAKI

  　View Summary

  In this study, we aimed to evaluate potential marker of metastasis in breast cancer. Reactive oxygen species, produced from NADPH oxidase (NOX), affect cancer metastasis and accelerate cell migration. Stanniocalcin-1 (STC-1), a regulator of calcium metabolism, is highly expressed in tumor cells. Therefore, we focused our research on calcium-dependent NOX5 and STC-1 molecules. High levels of STC-1 in breast cancer cells accelerated cell motility and enhanced lung metastasis in mice. However, no difference in cell migration was observed with high levels of NOX5. Thus, we conclude that STC-1 enhances the metastatic potential of breast cancer cells and our results suggest that STC-1 may have utility as a novel prediction marker of metastasis.

• Clinical study on the effect of androgen to organs other genitalis

  Grant-in-Aid for Scientific Research (C)

  Project Year :

  2007

  -

  2008

   

  KOBAYASHI Ko, MASUMORI Naoya, TAKAHASHI Satoshi

  　View Summary

  Androgenの減少は骨に対しては代謝の亢進につながることが明らかとなったが、短期間では骨粗鬆症の発症のレベルまでは骨密度の減少はなかった。握力などの筋力には経過中に変化を認めなかった。メタボリック症候群で注目される物質のひとつであるアディポネクチンも経過中は両群に変化を認めなかった。脳に対する影響として、認知障害の有無をミニメンタルステート検査を行ったが経過中に両群とも変化は確認できず、認知能に対する影響は確認できなかった。このように、アンドロゲンの低下が早期より影響を及ばす臓器としては生殖器以外では骨が注目される結果であった。

• The relationship of the inflammatory infiltrating cell in prostate grand and chemokine

  Grant-in-Aid for Scientific Research (C)

  Project Year :

  2001

  -

  2003

   

  ITOH Naoki, TAKAHASHI Satoshi, MASUMORI Naoya, TSUKAMOTO Taiji, HOTTA Hiroshi

  　View Summary

  (1)The pathological study of inflammatory cell infiltration in the prostate in patients Patients entered into the study received radical cystoprostatectomy because of invasive bladder cancer in our institute from 1998 to 2000. The infiltrating cell type was assessed by immunostaining using a series of antibodies CD2O, CD45RO, CD68,S-100. The types of infiltrating cells were dominantly T cells. Inflammatory cell infiltration was seen in 32.4% cases. (2)Antimicrobial agents and suppression of cytokine secretion of prostate cancer cell line. PC-3,a human prostate cancer cell lines, was used for the experiment. IL-8 concentrations of the supematants were increased depending on the concentrations of Mycoplasma hominis. The mRNA of Toll like receptor-2 and Toll like receptor4 were demonstrated by RT-PCR in PC-3 cell lines. The activities of NF-κB were increased depending on the concentration of Mycoplasma hominisa. These data show that the signals from Toll-like receptor up the activities of NF-κB and lead to produce of IL-8. The influence of GFLX on IL-8 mRNA expression was analyzed using RT-PCR. GFLX significantly attenuated the TNF-α induced IL-8 mRNA level. GFLX suppresses the secretion of IL-8 from PC-3 in a dose-dependent manner. The results may imply that GFLX has an anti-inflammatory effect on chronic prostatitis.

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