TAKAHASHI Satoshi

写真a

Affiliation

School of Medicine, Department of Clinical Laboratory Medicine

Job title

Professor
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Education 【 display / non-display 】

  • 2015
    -
    Now

    Sapporo Medical University  

  • 1992
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    2015

    Sapporo Medical University  

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    1992

    Sapporo Medical University  

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Degree 【 display / non-display 】

  • 1, Bactericidal effect of levofloxacin on strains with equal susceptibility in an in vitro urinary bladder model. 2, Changes in susceptibility of Pseudomonas aeruginosa to gatifloxacin and carbapenem in an in vitro urinary bladder model.

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Professional Memberships 【 display / non-display 】

  • 2015
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    Now

    Japanese Society of Laboratory Medicine

  • 2015
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    Now

    The Japan Association for Clinical Laboratory Science

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    American Sexually Transmitted Diseases Association

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    Japanese Association for Infectious Diseases

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    Japanese Society of Endourology and ESWL

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Research Areas 【 display / non-display 】

  • Life sciences   Infectious disease  

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Affiliation 【 display / non-display 】

  • Sapporo Medical University   School of Medicine, Department of Infection Control and Laboratory Medicine  

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Research Interests 【 display / non-display 】

  • Urinary tract infection, sexually transmitted infection, clinical laboratory medicine

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Papers 【 display / non-display 】

  • Pseudo-outbreak of Mycobacterium lentiflavum at a general hospital in Japan.

    Yutaro Nagano, Koji Kuronuma, Yasuo Kitamura, Kanami Nagano, Hayato Yabe, Sayaka Kudo, Toyotaka Sato, Shinya Nirasawa, Mami Nakae, Motohiro Horiuchi, Shin-Ichi Yokota, Yoshihiro Fujiya, Atsushi Saito, Satoshi Takahashi, Hirofumi Chiba

    Infection control and hospital epidemiology     1 - 7  2023.04  [International journal]

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    BACKGROUND: Mycobacterium lentiflavum is a slow-growing nontuberculous mycobacterium that is widely distributed in soil and water systems, but it is sometimes pathogenic to humans. Although cases of M. lentiflavum infections are rare, 22 isolates of M. lentiflavum were identified at a single hospital in Japan. We suspected a nosocomial outbreak; thus, we conducted transmission pattern and genotype analyses. METHODS: Cases of M. lentiflavum isolated at Kushiro City General Hospital in Japan between May 2020 and April 2021 were analyzed. The patient samples and environmental culture specimens underwent whole-genome sequencing (WGS). Additionally, we retrospectively collected clinical data from patient medical records. RESULTS: Altogether, 22 isolates of M. lentiflavum were identified from sputum and bronchoalveolar lavage samples. Clinically, the instances with M. lentiflavum isolates were considered contaminants. In the WGS analysis, 19 specimens, including 18 patient samples and 1 environmental culture from the hospital's faucet, showed genetic similarity. The frequency of M. lentiflavum isolation decreased after we prohibited the use of taps where M. lentiflavum was isolated. CONCLUSIONS: WGS analysis identified that the cause of M. lentiflavum pseudo-outbreak was the water used for patient examinations, including bronchoscopy.

    DOI PubMed

  • Analysis of diagnostic performance and factors causing nonspecific reactions in SARS-CoV-2 rapid antigen detection tests.

    Natsuki Narumi, Takashi Kondo, Yuki Sato, Yuki Katayama, Shinya Nirasawa, Masachika Saeki, Yuki Yakuwa, Yoshihiro Fujiya, Koji Kuronuma, Satoshi Takahashi

    Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy   29 ( 2 ) 157 - 162  2023.02  [International journal]

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    INTRODUCTION: Early diagnosis and appropriate infection control are important to prevent the transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this study, we aimed to assess the diagnostic performance of SARS-CoV-2 rapid antigen detection (RAD) tests and the factors that cause nonspecific reactions. METHODS: Nasopharyngeal swab specimens (n = 100), sputum specimens (n = 10), and lithium-heparin plasma samples (n = 100) were collected. We evaluated Espline®SARS-CoV-2 (Espline) and SARS-CoV-2 Rapid Antigen Test that also known as STANDARD Q® (STANDARD Q), with reverse transcription-polymerase chain reaction (RT-PCR) and Lumipulse® Presto SARS-CoV-2 Ag as reference tests. In addition, we investigated the effects of inadequate pretreatment methods and five potential causes of nonspecific reactions. RESULTS: The sensitivities of Espline and STANDARD Q were 60% and 57%, respectively, and their specificity was 100%. It was confirmed that the judgment line for the positive insufficiently mixed specimens was faint. A false-positive result was observed with STANDARD Q when sputum was used as a specimen to investigate judgment the effect of viscosity. CONCLUSIONS: Espline and STANDARD Q show good sensitivity for specimens with Ct values less than 25, but specimens collected within 9 days of symptom onset may still give false negatives. The test should be performed carefully, and the results should be judged comprehensively, taking into account clinical symptoms and patient background.

    DOI PubMed

  • Effect of albumin measurement methods on the albumin-bilirubin grade.

    Makito Tanaka, Shingo Tanaka, Ema Suzuki, Ryo Kobayashi, Satoshi Takahashi

    Annals of clinical biochemistry     45632221143685 - 45632221143685  2022.12  [International journal]

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    BACKGROUND: Albumin-bilirubin (ALBI) grade is an index of liver function based on total bilirubin (T-BIL) and albumin levels, and its usefulness has been widely reported. This study aimed to investigate the effect of different methods of measuring T-BIL and albumin levels on the ALBI grade in patients with liver disease. METHODS: In total, 170 patients from our hospital were included in this study. Correlations between T-BIL levels measured using the vanadate oxidation and enzymatic methods were analysed. Similarly, a correlation analysis of albumin levels between the bromocresol green (BCG) and modified bromocresol purple (BCP) methods was performed. Additionally, the ALBI grade was calculated for patients with liver disease, and the differences between each method of albumin measurement were compared. RESULTS: No differences were observed in the measured T-BIL values between the two methods. Contrastingly, the albumin levels of 100 random samples and 70 liver disease patients obtained using the modified BCP method were significantly lower than those measured using the BCG method. The rate of change in the modified ALBI grade between the BCG and BCP methods was 25.7%. CONCLUSIONS: Caution should be taken when comparing ALBI grades with those measured by other facilities because the method of albumin measurement can affect the ALBI grade. Standardization of albumin measurement is needed worldwide.

    DOI PubMed

  • Study of post-opening stability of active ingredients in hand sanitizers.

    Ryo Kobayashi, Ryosei Murai, Yuki Sato, Mami Nakae, Shinya Nirasawa, Koichi Asanuma, Koji Kuronuma, Satoshi Takahashi

    Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy   28 ( 12 ) 1605 - 1609  2022.12  [International journal]

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    INTRODUCTION: Hand disinfection plays an important role in infection control. Currently, hand sanitizers containing ethanol and chlorhexidine gluconate as active ingredients are widely used. Most of hand sanitizers have a defined expiration date for use. However, there was no rule about the expiration date after opening defined with the evidence. Therefore, we examined the fluctuation of active ingredients and disinfection effect after opening the bottle. METHOD: Twelve hand sanitizers from 44 to 921 days after opening set in different places in the hospital were examined and unopened hand sanitizer used as a control. Chlorhexidine gluconate and ethanol of each samples were measured by high performance liquid chromatography and gas chromatography, respectively. The correlation between the concentration of each ingredient obtained and the number of days after opening, bottle weight, storage temperature and humidity was analyzed. A time-kill test based on ASTM E2315-03 was performed to confirm the actual disinfection effect. RESULTS: It was observed that active ingredients had not been decreased up to 921 days after opening and were not affected by storage conditions after opening. In addition, a decrease of disinfection effect was not observed in any sample. CONCLUSIONS: We found that hand sanitizers do not need to be discard after a number of days have passed because the active ingredients are retained even after opening in it.

    DOI PubMed

  • Viral load may impact the diagnostic performance of nasal swabs in nucleic acid amplification test and quantitative antigen test for SARS-CoV-2 detection.

    Yoshihiro Fujiya, Yuki Sato, Yuki Katayama, Shinya Nirasawa, Mikako Moriai, Masachika Saeki, Yuki Yakuwa, Ikumi Kitayama, Koichi Asanuma, Koji Kuronuma, Satoshi Takahashi

    Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy   28 ( 11 ) 1590 - 1593  2022.11  [International journal]

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    INTRODUCTION: Compared to nasopharyngeal swabs (NPS), there has been insufficient evaluation of the diagnostic performance of nasal swabs (NS) for the detection of severe acute respiratory coronavirus 2 (SARS-CoV-2) in the nucleic acid amplification test (NAAT) and quantitative SARS-CoV-2 antigen test (QAT). METHODS: We prospectively compared healthcare worker-collected and flocked NS within nine days after symptom onset to paired NPS to detect SARS-CoV-2 in NAAT and QAT on the fully automated Lumipulse system. The agreement between sample types was evaluated, and cycle threshold (Ct) values and antigen levels were used as surrogate viral load measures. RESULTS: Sixty sets of NPS and NS samples were collected from 40 patients with COVID-19. The overall agreements between NAAT and QAT samples were 76.7% and 65.0%, respectively. In NAAT, the Ct value of NS was significantly higher, 5.9, than that of NPS. Thirty-nine (95.1%) NS tested positive in 41 positive-paired NPS with Ct ≤ 30. The negative correlation was observed between antigen levels of NS in QAT and Ct values of NS in NAAT (r = -0.88). In QAT, the antigen level of NS was significantly lower than that of NPS. Thirty-six (90.0%) NS tested positive in 40 positive-paired NPS with antigen levels >100 pg/mL, which were collected significantly earlier than those with antigen levels ≤100 pg/mL. CONCLUSIONS: In NAAT and QAT, NS had limited performance in detecting SARS-CoV-2 compared to NPS. However, NS may be helpful for patients with COVID-19 with high viral loads or those in the early stages of the illness.

    DOI PubMed

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Misc 【 display / non-display 】

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Research Projects 【 display / non-display 】

  • Epidemiological and therapeutical study of pathogens associated to non-gonococcal urethritis

    Grant-in-Aid for Scientific Research (B)

    Project Year :

    2013.04
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    2016.03
     

    MATSUMOTO TETSURO, HAMASUNA Ryoichi, TAKAHASHI Satoshi, FUKUDA Kazumasa

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    From the specimens of male urethritis, many kinds of microorganisms are detected, but the pathogenicities of microorganisms except for N. gonorrhoeae or C. trachomatis has not been determined. As the new approach, the clone library method was used for analysis of microorganisms of urethritis. This method added quantitative concept to qualitative PCR methods. By this method, the pathogenicities of N. gonorrhoeae adn M. genitalium to the male urethra was confirmed. In addition, it was found that H. influenzae and N. meningitidis would be possible pathogens for male urethritis. By the examination of microorganisms in the oral cavities of patients with male urethritis, it was found that causative bacteria of male urethritis and bacteria of the resident flora in the vagina were detected in the oral cavities of male patients. these indicated that oral sex is closely related to STIs. When we treat male urethritis, we have to eradicate microorganisms in both the urethra and oral cavities.

  • Clinical study on the effect of androgen to organs other genitalis

    Grant-in-Aid for Scientific Research (C)

    Project Year :

    2007
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    2008
     

    KOBAYASHI Ko, MASUMORI Naoya, TAKAHASHI Satoshi

  • The relationship of the inflammatory infiltrating cell in prostate grand and chemokine

    Grant-in-Aid for Scientific Research (C)

    Project Year :

    2001
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    2003
     

    ITOH Naoki, TAKAHASHI Satoshi, MASUMORI Naoya, TSUKAMOTO Taiji, HOTTA Hiroshi

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    (1)The pathological study of inflammatory cell infiltration in the prostate in patients Patients entered into the study received radical cystoprostatectomy because of invasive bladder cancer in our institute from 1998 to 2000. The infiltrating cell type was assessed by immunostaining using a series of antibodies CD2O, CD45RO, CD68,S-100. The types of infiltrating cells were dominantly T cells. Inflammatory cell infiltration was seen in 32.4% cases. (2)Antimicrobial agents and suppression of cytokine secretion of prostate cancer cell line. PC-3,a human prostate cancer cell lines, was used for the experiment. IL-8 concentrations of the supematants were increased depending on the concentrations of Mycoplasma hominis. The mRNA of Toll like receptor-2 and Toll like receptor4 were demonstrated by RT-PCR in PC-3 cell lines. The activities of NF-κB were increased depending on the concentration of Mycoplasma hominisa. These data show that the signals from Toll-like receptor up the activities of NF-κB and lead to produce of IL-8. The influence of GFLX on IL-8 mRNA expression was analyzed using RT-PCR. GFLX significantly attenuated the TNF-α induced IL-8 mRNA level. GFLX suppresses the secretion of IL-8 from PC-3 in a dose-dependent manner. The results may imply that GFLX has an anti-inflammatory effect on chronic prostatitis.

  • Study on the regulation mechanism of proliferation and differentiation of human prostatic stromal cells: Effects of TGF-βs on human prostatic myofibroblast and α-adrenergic receptor.

    Grant-in-Aid for Scientific Research (C)

    Project Year :

    1999
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    2001
     

    TAKAHASHI Satoshi, TAKAHASHI Atsushi, MASUMORI Naoya, ITOH Naoki, KUNISHIMA Yasuharu, SASAMURA Hiroto

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    In order to study the effects of TGF-βs on human prostatic stromal cells, we purified human prostatic myofibroblast using specimens that was taken by operations and established culture system of these cells. These cells were immunohistochemically recognized as myofibroblast according to the findings of positive staining of a-smooth muscle actin and negative staining of both myosin and desmin. The expressions of TCF-β receptor I, II mRNA were confirmed by RT-PCR in these cells. After the treatment of various concentrations of human TGF-β1, 2, 3, it was recognized that these cells expressed both myosin and desmin. These findings suggested that TGF-βs could differentiate myofibroblast to smooth muscle. On the other hand, TGF-β1, 2, 3 inhibited proliferation of myofibroblast by dose-dependent manner. It has been well known that there are three subtypes of α1-receptor in human prostatic tissue; 1a, 1b and 1d, however the distribution of these subtypes in myofibroblast and smooth muscle cells has not been clarified. Using myofibroblast that was established by us and human prostatic smooth muscle that was commercially available, the expressions of 1a, 1b, 1d subtype mRNA were quantitatively analyzed by real time RT-PCR. The expression of 1a was recognized in smooth muscle only, and both 1b and 1d were determined in myofibroblast and smooth muscle cells. Based on these findings, it was speculated that the expression of ala receptor mRNA was induced during the differentiation from myofibroblast to smooth muscle. To clarify the effect of TGF-β1 on the expression of α1 receptor in myofibroblast and smooth muscle, the expressios of α1a, 1b, 1d receptor mRNA were quantitatively analyzed by real time RT-PCR. Moreover, α1 receptor binding assay was performed using [^<125 >I] HEAT as a ligand. The expression of ala was detected in these cells cultured under the condition without TGF-β1. The expressions of 1b and 1d without TGF-β were significantly higher 0.13 and 0.09 times respectively than with TGF-β1. Bmax of [^^<125>I]HEAT (300pM) was 37.5 fmol/mg protein in the medium without TGF-β1 and 21.3fmol/mg protein in those with TGF-β1. It was concluded that TGF-βs could have human prostatic myofibroblast differentiate to smooth muscle cell. On the other hand, the function of smooth muscle was undifferentiated by TGF-β1, because the expression of al receptor was suppressed by it. Based on these results, it was speculated that the regulatory mechanisms of morphological differentiation might be different from these of functional differentiation in human prostatic stroma.

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