Education 【 display / non-display 】

Education 【 display / non-display 】

• 2015

  -

  Now

  Sapporo Medical University   医学部   感染制御・臨床検査医学講座  

• 1992

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  2015

  Sapporo Medical University   医学部   泌尿器科学講座  

• 　

  -

  1992

  Sapporo Medical University  

Degree 【 display / non-display 】

Degree 【 display / non-display 】

• 1, Bactericidal effect of levofloxacin on strains with equal susceptibility in an in vitro urinary bladder model. 2, Changes in susceptibility of Pseudomonas aeruginosa to gatifloxacin and carbapenem in an in vitro urinary bladder model.

Professional Memberships 【 display / non-display 】

Professional Memberships 【 display / non-display 】

• 2015

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  Now

  Japanese Society of Laboratory Medicine

• 2015

  -

  Now

  The Japan Association for Clinical Laboratory Science

• 　

  　

  　

  International Union against Sexually Transmitted Infections

• 　

  　

  　

  Japanese Society of Chemotherapy

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  American Society for Microbiology

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Research Areas 【 display / non-display 】

Research Areas 【 display / non-display 】

• Life sciences   Infectious disease  

Affiliation 【 display / non-display 】

Affiliation 【 display / non-display 】

• Sapporo Medical University   School of Medicine, Department of Infection Control and Laboratory Medicine  

Research Interests 【 display / non-display 】

Research Interests 【 display / non-display 】

• Urinary tract infection, sexually transmitted infection, clinical laboratory medicine

Papers 【 display / non-display 】

Papers 【 display / non-display 】

• Traces of pandemic fluoroquinolone-resistant Escherichia coli clone ST131 transmitted from human society to aquatic environments and wildlife in Japan

  Toyotaka Sato, Kojiro Uemura, Mitsuru Yasuda, Aiko Maeda, Toshifumi Minamoto, Kazuki Harada, Michiyo Sugiyama, Shiori Ikushima, Shin-ichi Yokota, Motohiro Horiuchi, Satoshi Takahashi, Testuo Asai

  One Health ( Elsevier BV )  18   100715 - 100715  2024.06  [Refereed]  [International journal]

  　View Summary

  Transmission of antimicrobial-resistant bacteria among humans, animals, and the environment is a growing concern worldwide. The distribution of an international high-risk fluoroquinolone-resistant Escherichia coli clone, ST131, has been documented in clinical settings. However, the transmission of ST131 from humans to surrounding environments remains poorly elucidated. To comprehend the current situation and identify the source of ST131 in nature, we analyzed the genetic features of ST131 isolates from the aquatic environment (lake/river water) and wildlife (fox, raccoon, raccoon dog, and deer) and compared them with the features of isolates from humans in Japan using accessory and core genome single nucleotide polymorphism (SNP) analyses. We identified ST131 isolates belonging to the same phylotype and genome clusters (four of eight clusters were concomitant) with low SNP distance between the human isolates and those from the aquatic environment and wildlife. These findings warn of ST131 transmission between humans and the surrounding environment in Japan.

  DOI PubMed

• 当院改修時におけるアスペルギルス対策の評価

  黒沼 幸治, 中江 舞美, 韮澤 慎也, 富樫 篤生, 藤谷 好弘, 齋藤 充史, 高橋 聡

  感染症学雑誌 ( (一社)日本感染症学会 )  98 ( 2 ) 281 - 281  2024.03

• Affinity of β-Lactam Antibiotics for Neisseria gonorrhoeae Penicillin-Binding Protein 2 Having Wild, Cefixime-Reduced-Susceptible, and Cephalosporin (Ceftriaxone)-Resistant penA Alleles.

  Yoshiki Hiyama, Soh Yamamoto, Toyotaka Sato, Noriko Ogasawara, Naoya Masumori, Satoshi Takahashi, Shin-Ichi Yokota

  Microbial drug resistance (Larchmont, N.Y.)    2024.01  [International journal]

  　View Summary

  Multidrug-resistant Neisseria gonorrhoeae is a serious concern worldwide. Resistance to β-lactam antibiotics occurs through mutations in penicillin-binding proteins (PBPs), acquisition of β-lactamases, and alteration of antibiotic penetration. Mosaic structures of penA, which encodes PBP2, play a major role in resistance to β-lactams, especially cephalosporins. Ceftriaxone (CRO) is recognized as the only satisfiable antibiotic for the treatment of gonococcal infections; however, CRO-resistant isolates have emerged in the community. Here, we examined the affinity of β-lactam antibiotics for recombinant PBP2 in a competition assay using fluorescence-labeled penicillin. We found no or little difference in the affinities of penicillins and meropenem (MEM) for PBP2 from cefixime (CFM)-reduced-susceptible strain and cephalosporin-resistant strain. However, the affinity of cephalosporins, including CRO, for PBP2 from the cephalosporin-resistant strain was markedly lower than that for PBP2 from the CFM-reduced-susceptible-resistant strain. Notably, piperacillin (PIP) showed almost the same affinity for PBP2 from penicillin-susceptible, CFM-reduced-susceptible, and cephalosporin (including CRO)-resistant strains. Thus, PIP/tazobactam and MEM are candidate antibiotics for the treatment of CRO-resistant/multidrug-resistant N. gonorrhoeae.

  DOI PubMed

• 手洗器の水栓口や給水システムの汚染による緑膿菌の感染伝播

  藤谷 好弘, 中江 舞美, 佐藤 勇樹, 韮澤 慎也, 富樫 篤生, 齋藤 充史, 黒沼 幸治, 高橋 聡

  感染症学雑誌 ( (一社)日本感染症学会 )  98 ( 1 ) 119 - 119  2024.01

• Tazobactam/ceftolozane and tobramycin combination therapy in extensively drug-resistant Pseudomonas aeruginosa infections in severe burn injury: a case report.

  Yuta Ibe, Ryuichiro Kakizaki, Hirotoshi Inamura, Tomoyuki Ishigo, Yoshihiro Fujiya, Hiroyuki Inoue, Shuji Uemura, Satoshi Fujii, Satoshi Takahashi, Eichi Narimatsu, Masahide Fukudo

  Journal of pharmaceutical health care and sciences   9 ( 1 ) 25 - 25  2023.08  [International journal]

  　View Summary

  BACKGROUND: Combination therapy with tazobactam/ceftolozane (TAZ/CTLZ) and high-dose aminoglycosides has been reported to be efficacious in extensively drug-resistant (XDR)-Pseudomonas aeruginosa infection. However, there are no reports of efficacy in XDR-P. aeruginosa infection for combination therapy with low-dose aminoglycosides and TAZ/CTLZ. Herein, we describe a rare case of severe burn injury patients with persistent bacteremia due to XDR-P. aeruginosa, which was successfully treated with TAZ/CTLZ and low-dose tobramycin (TOB). CASE PRESENTATION: A 31-year-old man was admitted to the intensive care unit with severe burn injury involving 52% of the total body surface area and a prognostic burn index of 79.5. The patient had recurrent bacterial infections since admission, and blood cultures collected on the 37th day of admission revealed the presence of P. aeruginosa strains that were resistant to all β-lactams and amikacin (AMK). The results of the antimicrobial synergistic study showed no synergistic effect of low-dose meropenem (MEPM) and AMK combination therapy. The patient had acute renal failure, and it was difficult to increase the dose of MEPM and AMK, respectively. Thus, we initiated TAZ/CTLZ 1.5 g/8 h instead of the AMK and MEPM combination therapy on the 43rd day of hospitalization. Low-dose TAZ/CTLZ was continued because of prolonged renal dysfunction and resulted in a transient clinical improvement. However, the dosage of TAZ/CTLZ could be increased as the renal function improved, but despite an increased TAZ/CTLZ dose, bacteremia persisted, and the blood cultures remained positive. Thus, TOB was added to TAZ/CTLZ at low doses for synergistic effect against Gram-negative bacteria. Blood cultures collected after initiation of combination therapy with TAZ/CTLZ and low-dose TOB were negative on two consecutive follow-up evaluations. Thereafter, although the patient had several episodes of fever and increased inflammatory response, blood cultures consistently tested negative, and all of the wounds healed. On the 93rd day, due to the good healing progress, the patient was transferred to another hospital. CONCLUSIONS: TAZ/CTLZ and low-dose TOB combination therapy showed the potential for synergistic effects. Our present report suggests a novel synergistic treatment strategy for rare cases that are refractory to the treatment of infections, such as XDR-P. aeruginosa infection.

  DOI PubMed

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Misc 【 display / non-display 】

Misc 【 display / non-display 】

• COVID-19ワクチン接種後感染者1事例の抗体獲得推移

  中江 舞美, 藤谷 好弘, 藤居 賢, 黒沼 幸治, 高橋 聡

  日本環境感染学会総会プログラム・抄録集 ( (一社)日本環境感染学会 )  36回   311 - 311  2021.09

• 新型コロナウイルス感染症専用病棟の職員の感染リスクにかかる後向きコホート研究

  藤谷 好弘, 中江 舞美, 中村 広士, 黒沼 幸治, 高橋 聡

  日本環境感染学会総会プログラム・抄録集 ( (一社)日本環境感染学会 )  36回   303 - 303  2021.09

• 新型コロナウイルス感染症におけるファビピラビル投与の評価

  伊部 裕太, 藤居 賢, 石郷 友之, 國本 雄介, 藤谷 好弘, 黒沼 幸治, 高橋 聡

  日本化学療法学会雑誌 ( (公社)日本化学療法学会 )  69 ( Suppl.A ) 254 - 254  2021.04

• 鼻腔ぬぐい液および抗原定量検査の臨床的有効性に関する検討

  藤谷 好弘, 黒沼 幸治, 高橋 聡

  感染症学雑誌 ( (一社)日本感染症学会 )  95 ( 臨増 ) 251 - 251  2021.04

• 流産手術後に長期間の反復菌血症を呈しミュンヒハウゼン症候群が疑われた1例

  真里谷 奨, 長井 陽子, 藤谷 好弘, 黒沼 幸治, 高橋 聡

  感染症学雑誌 ( (一社)日本感染症学会 )  95 ( 臨増 ) 288 - 288  2021.04

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Awards 【 display / non-display 】

Awards 【 display / non-display 】

• 北海道医師会賞・北海道知事賞

  2018.10   北海道医師会・北海道  

• 第98回日本泌尿器科学会 総会賞

  2010.04  

• 第24回稲田賞

  2006   泌尿器科紀要  

• 第365回日本泌尿器科学会北海道地方会 地方会賞

  2005   日本泌尿器科学会北海道地方会  

• 平成14年度日本抗生物質学術協議会・ファイザー感染症研究助成（海外留学助成）

  2002   日本抗生物質学術協議会  

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Research Projects 【 display / non-display 】

Research Projects 【 display / non-display 】

• 淋菌薬剤感受性サーベイランスによる薬剤耐性淋菌に対する有効な抗菌化学療法の検討

  基盤研究(C)

  Project Year :

  2022.04

  -

  2025.03

   

  安田 満, 高橋 聡, 佐藤 勇樹

• Epidemiological and therapeutical study of pathogens associated to non-gonococcal urethritis

  Grant-in-Aid for Scientific Research (B)

  Project Year :

  2013.04

  -

  2016.03

   

  MATSUMOTO TETSURO, HAMASUNA Ryoichi, TAKAHASHI Satoshi, FUKUDA Kazumasa

  　View Summary

  From the specimens of male urethritis, many kinds of microorganisms are detected, but the pathogenicities of microorganisms except for N. gonorrhoeae or C. trachomatis has not been determined. As the new approach, the clone library method was used for analysis of microorganisms of urethritis. This method added quantitative concept to qualitative PCR methods. By this method, the pathogenicities of N. gonorrhoeae adn M. genitalium to the male urethra was confirmed. In addition, it was found that H. influenzae and N. meningitidis would be possible pathogens for male urethritis. By the examination of microorganisms in the oral cavities of patients with male urethritis, it was found that causative bacteria of male urethritis and bacteria of the resident flora in the vagina were detected in the oral cavities of male patients. these indicated that oral sex is closely related to STIs. When we treat male urethritis, we have to eradicate microorganisms in both the urethra and oral cavities.

• Evaluation of stanniocalcin-1 and NADPH oxidase as marker of metastasis in breast cancer

  Grant-in-Aid for Scientific Research (C)

  Project Year :

  2012.04

  -

  2016.03

   

  TANAKA MAKI, TAKAHASHI SATOSHI, KOBAYASHI DAISUKE, WATANABE NAOKI, KURIBAYASHI KAGEAKI

  　View Summary

  In this study, we aimed to evaluate potential marker of metastasis in breast cancer. Reactive oxygen species, produced from NADPH oxidase (NOX), affect cancer metastasis and accelerate cell migration. Stanniocalcin-1 (STC-1), a regulator of calcium metabolism, is highly expressed in tumor cells. Therefore, we focused our research on calcium-dependent NOX5 and STC-1 molecules. High levels of STC-1 in breast cancer cells accelerated cell motility and enhanced lung metastasis in mice. However, no difference in cell migration was observed with high levels of NOX5. Thus, we conclude that STC-1 enhances the metastatic potential of breast cancer cells and our results suggest that STC-1 may have utility as a novel prediction marker of metastasis.

• Clinical study on the effect of androgen to organs other genitalis

  Grant-in-Aid for Scientific Research (C)

  Project Year :

  2007

  -

  2008

   

  KOBAYASHI Ko, MASUMORI Naoya, TAKAHASHI Satoshi

  　View Summary

  Androgenの減少は骨に対しては代謝の亢進につながることが明らかとなったが、短期間では骨粗鬆症の発症のレベルまでは骨密度の減少はなかった。握力などの筋力には経過中に変化を認めなかった。メタボリック症候群で注目される物質のひとつであるアディポネクチンも経過中は両群に変化を認めなかった。脳に対する影響として、認知障害の有無をミニメンタルステート検査を行ったが経過中に両群とも変化は確認できず、認知能に対する影響は確認できなかった。このように、アンドロゲンの低下が早期より影響を及ばす臓器としては生殖器以外では骨が注目される結果であった。

• The relationship of the inflammatory infiltrating cell in prostate grand and chemokine

  Grant-in-Aid for Scientific Research (C)

  Project Year :

  2001

  -

  2003

   

  ITOH Naoki, TAKAHASHI Satoshi, MASUMORI Naoya, TSUKAMOTO Taiji, HOTTA Hiroshi

  　View Summary

  (1)The pathological study of inflammatory cell infiltration in the prostate in patients Patients entered into the study received radical cystoprostatectomy because of invasive bladder cancer in our institute from 1998 to 2000. The infiltrating cell type was assessed by immunostaining using a series of antibodies CD2O, CD45RO, CD68,S-100. The types of infiltrating cells were dominantly T cells. Inflammatory cell infiltration was seen in 32.4% cases. (2)Antimicrobial agents and suppression of cytokine secretion of prostate cancer cell line. PC-3,a human prostate cancer cell lines, was used for the experiment. IL-8 concentrations of the supematants were increased depending on the concentrations of Mycoplasma hominis. The mRNA of Toll like receptor-2 and Toll like receptor4 were demonstrated by RT-PCR in PC-3 cell lines. The activities of NF-κB were increased depending on the concentration of Mycoplasma hominisa. These data show that the signals from Toll-like receptor up the activities of NF-κB and lead to produce of IL-8. The influence of GFLX on IL-8 mRNA expression was analyzed using RT-PCR. GFLX significantly attenuated the TNF-α induced IL-8 mRNA level. GFLX suppresses the secretion of IL-8 from PC-3 in a dose-dependent manner. The results may imply that GFLX has an anti-inflammatory effect on chronic prostatitis.

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