Misc - 秋山　幸功

尾骨部滑液包炎の1例

吉藤 和久, 小柳 泉, 馬場 雄大, 原口 浩一, 秋山 幸功, 山下 太郎, 小松 克也, 杉野 寿哉, 池田 英之, 宝金 清博

小児の脳神経 ( (一社)日本小児神経外科学会 )  31 ( 2 ) 150 - 150  2006年04月

もやもや病の家系内有病率の再検討

秋山 幸功, 宝金 清博, 野中 雅, 馬場 雄大

脳卒中 ( (一社)日本脳卒中学会 )  28 ( 1 ) 133 - 133  2006年03月

急性期脳梗塞における血圧の自然変化(病型別にみた血圧の推移)

秋山 幸功, 山村 明範, 中川 俊男, 宝金 清博

脳卒中 ( (一社)日本脳卒中学会 )  28 ( 1 ) 220 - 220  2006年03月

急性期脊髄損傷に対する治療 急性期頸椎頸髄損傷に対する治療 ガイドラインから

小柳 泉, 吉藤 和久, 秋山 幸功, 宝金 清博, 今村 博幸, 飛騨 一利, 岩崎 喜信

日本脳神経外科救急学会プログラム・抄録集 ( (NPO)日本脳神経外科救急学会 )  11回   69 - 69  2006年01月

病院到着前心停止患者に対する早期除細動および循環補助療法の神経機能予後について

秋山 幸功, 長谷 守, 浅井 康文, 宝金 清博

日本脳神経外科救急学会プログラム・抄録集 ( (NPO)日本脳神経外科救急学会 )  11回   89 - 89  2006年01月

Integration of engrafted Schwann cells into injured peripheral nerve: Axonal association and nodal formation on regenerated axons

C Radtke, Y Akiyama, KL Lankford, PM Vogt, DS Krause, JD Kocsis

NEUROSCIENCE LETTERS ( ELSEVIER IRELAND LTD )  387 ( 2 ) 85 - 89  2005年10月

　概要を見る

Transplantation of myelin-forming cells can remyelinate axons, but little is known of the sodium channel organization of axons myelinated by donor cells. Sciatic nerve axons of female wild type mice were transected by a crush injury and Schwann cells (SCs) from green fluorescence protein (GFP)-expressing male mice were transplanted adjacent to the crush site. The male donor cells were identified by GFP fluorescence and fluorescence in situ hybridization (FISH) for Y chromosome. In nerves of GFP-expressing mice, GFP was observed in the axoplasm and in the cytoplasmic compartments of the Schwann cells, but not in the myelin. Following transplantation of GFP-SCs into crushed nerve of wild type mice, immuno-electron microscopic analysis indicated that GFP was observed in the cytoplasmic compartments of engrafted Schwann cells which formed myelin. Nodal and paranodal regions of the axons myelinated by the GFP-SCs were identified by Na-v 1.6 sodium channel and Caspr immunostaining, respectively. Nuclear identification of the Y chromosome by FISH confirmed the donor origin of the myelin-forming cells. These results indicate that engrafted GFP-SCs participate in myelination of regenerated peripheral nerve fibers and that Na-v 1.6 sodium channel, which is the dominant sodium channel at normal nodes, is reconstituted on the regenerated axons. (C) 2005 Elsevier Ireland Ltd. All rights reserved.

DOI

Autologous transplantation of expanded neural precursor cells into the demyelinated monkey spinal cord

S Oka, O Honmou, Y Akiyama, M Sasaki, K Houkin, K Hashi, JD Kocsis

BRAIN RESEARCH ( ELSEVIER SCIENCE BV )  1030 ( 1 ) 94 - 102  2004年12月

　概要を見る

The objective of this study was to establish if neural precursor cells could safely be developed from biopsy of the subventricular zone (SVZ) in the non-human primate (marmoset), and to determine their myelinating potential after autologous transplantation into a demyelinated lesion. Small amounts of tissue were safely collected from the subventricular-subependymal zone of the adult primate brain under ultrasonography without any neurological deficit. Neural precursor cells were isolated and expanded in the presence of mitogen in vitro. The dorsal columns of the adult marmoset spinal cord were demyelinated by X-irradiation and intraspinal injections of ethidium bromide in the center of the radiation field. Cell suspensions of the neural precursors were microinjected through a micropipette into the demyelinated lesion site in the spinal cord. Lesions were histologically examined 3 weeks after transplantation. Light and electron microscopic examination of plastic embedded sections revealed a significant number of myelinating profiles in the transplantation zone; no myelination was observed in control lesions. The myelinated axons had predominantly peripheral patterns of myelination. These results demonstrate that autologous transplantation of neural precursor cells in the adult nonhuman primate can remyelinate demyelinated central nervous system (CNS) axons, thus suggesting the potential utility of such an approach in demyelinating lesions in humans. (C) 2004 Elsevier B.V. All rights reserved.

DOI

Neural precursors as a cell source to repair the demyelinated spinal cord

JD Kocsis, Y Akiyama, C Radtke

JOURNAL OF NEUROTRAUMA ( MARY ANN LIEBERT INC PUBL )  21 ( 4 ) 441 - 449  2004年04月

　概要を見る

Schwann cells and neural precursor cells derived from adult human brain (subventricular zone) and from bone marrow were studied anatomically and physiologically after transplantation into the demyelinated rat spinal cord. All cell types formed myelin and restored conduction velocity. Following transection of the dorsal funiculus, Schwann cells and olfactory ensheathing cells facilitated axonal regeneration and restoration of conduction across the lesion site. There is discussion on the challenges of cell type selection and preparation for a potential clinical cell therapy study in human demyelinating diseases.

DOI

Bone marrow stem cell

Saisentan Series 29   29   83 - 86  2004年

Remyelination of the nonhuman primate spinal cord by transplantation of H-transferase transgenic adult pig olfactory ensheathing cells

RADTKE C

FASEB J   18   335 - 7  2004年

CiNii

脳の再生医療と臨床応用

最先端医療シリーズ30 神経内科   30   11 - 15  2004年

骨髄細胞を用いた再生医療

先端医療シリーズ29 脳神経外科   29   83 - 86  2004年

brain repair

Saisentan Series 30   30   11 - 15  2004年

Remyelination of the spinal cord following intravenous delivery of bone marrow cells

Y Akiyama, C Radtke, O Honmou, JD Kocsis

GLIA ( WILEY-LISS )  39 ( 3 ) 229 - 236  2002年09月

　概要を見る

Bone marrow contains a population of pluripotent cells that can differentiate into a variety of cell lineages, including neural cells. When injected directly into the demyelinated spinal cord they can elicit remyelination. Recent work has shown that following systemic delivery of bone marrow cells functional improvement occurs in contusive spinal cord injury and stroke models in rat. We report here that secondary to intravenous introduction of an acutely isolated bone marrow cell fraction (mononuclear fraction) from adult rat femoral bones separated on a density gradient, ultrastructurally defined remyelination occurs throughout a focal demyelinated spinal cord lesion. The anatomical pattern of remyelination was characteristic of both oligodendrocyte and Schwann cell myelination; conduction velocity improved in the remyelinated axons. When the injected bone marrow cells were transfected to express LacZ, beta-galactosidase reaction product was observed in some myelin-forming cells in the spinal cord. Intravenous injection of other myelin-forming cells (Schwann cells and olfactory ensheathing cells) or the residual cell fraction of the gradient did not result in remyelination, suggesting that remyelination was specific to the delivery of the mononuclear fraction. While the precise mechanism of the repair, myelination by the bone marrow cells or facilitation of an endogenous repair process, cannot be fully determined, the results demonstrate an unprecedented level of myelin repair by systemic delivery of the mononuclear cells.

DOI

Remyelination of the rat spinal cord by transplantation of identified bone marrow stromal cells

Y Akiyama, C Radtke, JD Kocsis

JOURNAL OF NEUROSCIENCE ( SOC NEUROSCIENCE )  22 ( 15 ) 6623 - 6630  2002年08月

　概要を見る

Bone marrow contains a population of stem-like cells that can differentiate into neurons or glia. Stromal cells from green fluorescent protein (GFP)-expressing mice were isolated by initial separation on a density gradient and then cultured as adherent cells on plastic that proliferated in culture to confluency with a typical flattened elongative morphology. The large majority of the isolated stromal cells were GFP expressing and immunopositive for collagen type I, fibronectin, and CD44. Transplantation of these cells by direct microinjection into the demyelinated spinal cord of the immunosuppressed rat resulted in remyelination. The remyelinated axons showed characteristics of both central and peripheral myelination as observed by electron microscopy; conduction velocity of the axons was improved. GFP-positive cells and myelin profiles were observed in the remyelinated spinal cord region, indicating that the donor-isolated stromal cells were responsible for the formation of the new myelin. The GFP-positive cells were colocalized with myelin basic protein-positive and P0-positive cellular elements. These findings indicate that cells contained within the stromal cell fraction of the mononuclear cell layer of bone marrow can form functional myelin during transplantation into demyelinated spinal cord.