Research Experience 【 display / non-display 】

Research Experience 【 display / non-display 】

• 2012

  　

  　

  Sapporo Medical University   附属総合情報センター   助教

  助教

Research Areas 【 display / non-display 】

Research Areas 【 display / non-display 】

• Life sciences   Neurosurgery  

• Life sciences   Healthcare management, medical sociology  

Affiliation 【 display / non-display 】

Affiliation 【 display / non-display 】

• Sapporo Medical University   附属総合情報センター   助教  

Research Interests 【 display / non-display 】

Research Interests 【 display / non-display 】

• 周産期

• 医療情報

• 語彙連鎖解析

• ユビキタス

• 過疎

display all >>

Papers 【 display / non-display 】

Papers 【 display / non-display 】

• Molecular evolution of vertebrate Toll-like receptors: Evolutionary rate difference between their leucine-rich repeats and their TIR domains

  Tomoko Mikami, Hiroki Miyashita, Shintaro Takatsuka, Yoshio Kuroki, Norio Matsushima

  GENE ( ELSEVIER SCIENCE BV )  503 ( 2 ) 235 - 243  2012.07  [Refereed]

  　View Summary

  Toll-like receptors (TLRs) that initiate an innate immune response contain an extracellular leucine rich repeat (LRR) domain and an intracellular Toll IL-receptor (TIR) domain. There are fifteen different TLRs in vertebrates. The LRR domains, which adopt a solenoid structure, usually have higher rates of evolution than do the TIR globular domains. It is important to understand the molecular evolution and functional roles of TLRs from this standpoint. Both pairwise genetic distances and Ka/Ks's (the ratios between non synonymous and synonymous substitution rates) were compared between the LRR domain and the TIR domain of 366 vertebrate TLRs from 96 species (from fish to primates). In fourteen members (TLRs 1, 2, 3, 4, 5, 6, 7, 8, 9, 11/12, 13, 14, 21, and 22/23) the LRR domains evolved significantly more rapidly than did the corresponding TIR domains. The evolutionary rates of the LRR domains are significantly different among these members; LRR domains from TLR3 and TLR7 from primates to fishes have the lowest rate of evolution. In contrast, the fifteenth member, TLR10, shows no significant differences; its TIR domain is not highly conserved. The present results suggest that TLR10 may have a different function in signaling from those other members and that a higher conservation of TLR3 and TLR7 may reflect a more ancient mechanism and/or structure in the innate immune response system. Gene conversions are suggested to have occurred in platypus TLR6 and TLR10. This study provides new insight about structural and functional diversification of vertebrate TLRs. (C) 2012 Elsevier B.V. All rights reserved.

  DOI PubMed

• Genome-wide Profiling of Chromatin Signatures Reveals Epigenetic Regulation of MicroRNA Genes in Colorectal Cancer

  Hiromu Suzuki, Shintaro Takatsuka, Hirofumi Akashi, Eiichiro Yamamoto, Masanori Nojima, Reo Maruyama, Masahiro Kai, Hiro-o Yamano, Yasushi Sasaki, Takashi Tokino, Yasuhisa Shinomura, Kohzoh Imai, Minoru Toyota

  CANCER RESEARCH ( AMER ASSOC CANCER RESEARCH )  71 ( 17 ) 5646 - 5658  2011.09  [Refereed]

  　View Summary

  Altered expression of microRNAs (miRNA) occurs commonly in human cancer, but the mechanisms are generally poorly understood. In this study, we examined the contribution of epigenetic mechanisms to miRNA dysregulation in colorectal cancer by carrying out high-resolution ChIP-seq. Specifically, we conducted genome-wide profiling of trimethylated histone H3 lysine 4 (H3K4me3), trimethylated histone H3 lysine 27 (H3K27me3), and dimethylated histone H3 lysine 79 (H3K79me2) in colorectal cancer cell lines. Combining miRNA expression profiles with chromatin signatures enabled us to predict the active promoters of 233 miRNAs encoded in 174 putative primary transcription units. By then comparing miRNA expression and histone modification before and after DNA demethylation, we identified 47 miRNAs encoded in 37 primary transcription units as potential targets of epigenetic silencing. The promoters of 22 transcription units were associated with CpG islands (CGI), all of which were hypermethylated in colorectal cancer cells. DNA demethylation led to increased H3K4me3 marking at silenced miRNA genes, whereas no restoration of H3K79me2 was detected in CGI-methylated miRNA genes. DNA demethylation also led to upregulation of H3K4me3 and H3K27me3 in a number of CGI-methylated miRNA genes. Among the miRNAs we found to be dysregulated, many of which are implicated in human cancer, miR-1-1 was methylated frequently in early and advanced colorectal cancer in which it may act as a tumor suppressor. Our findings offer insight into the association between chromatin signatures and miRNA dysregulation in cancer, and they also suggest that miRNA reexpression may contribute to the effects of epigenetic therapy. Cancer Res; 71(17); 5646-58. (C)2011 AACR.

  DOI PubMed

Misc 【 display / non-display 】

Misc 【 display / non-display 】

• 半導体素子のγ線照射による損傷

  田中 憲一, 加茂 憲一, 高塚 伸太朗

  放射線防護医療 ( 放射線防護医療研究会 )  ( 5 ) 32 - 35  2009.12

  CiNii

• Verification of the validity of the dosimeter 'Radtriage' for triage based on exposure dose

  田中 憲一, 高塚 伸太朗, 高田 純

  放射線防護医療 ( 放射線防護医療研究会 )  ( 4 ) 34 - 36  2008.12

  CiNii

• Regional Medicine supported by Information and communication Technology

  AKASHI Hirofumi, SHIMMI Takahiko, TAKATSUKA Shintaro, TOKURA Hajime, ASARI Toshimitsu, OHISHI Norikatsu, FUJIKAWA Kenji, NATORI Hiroshi, TATSUMI Haruyuki, SATOH Noriyuki

  IEICE technical report. ( The Institute of Electronics, Information and Communication Engineers )  108 ( 131 ) 41 - 46  2008.07

  　View Summary

  Due to the fact that people are spread over a very wide area in Hokkaido and medical infrastructure and manpower is concentrated in the urban areas, regional medicine supported by ICT is supposed to be the best answer to the problems. We have tried to develop and to estimate a lot of applications and infrastructure for medical information network systems through some projects, such as "Hokkaido Wide Area Medical Network System" project, financed by the MLIT (Ministry of Land, Infrastructure and Transport), Scientific research granted by Ministry of Health, Labor and Welfare "Home Health Care" project, financed by NEDO, "Strategic Information and Communications R & D Promotion Program" financed by MIC (Ministry of Internal Affairs and Communications) and SMU's "Regional Medicine Support" project. We performed medical data or images transmission, remote lectures by using video conference systems and home health care systems. Furthermore, we established security and stability network infrastructure with the use of IPsec, Regional IX, IPv6, TPA (Topological Addressing Policy) and VGN (Virtual Global Network) technologies. In this time we report accomplishments and problems up to the present.

  CiNii

• Sleep Apnea Syndrome detection based on heart rate variability analysis

  TAKATSUKA Shintaro, MURABAYASHI Shun, MITAMURA Yoshinori

  電気学会研究会資料. MBE, 医用・生体工学研究会   2005 ( 7 ) 9 - 12  2005.06

  CiNii

• Sleep Apnea Syndrome detection based on heart rate variability analysis

  TAKATSUKA Shintaro, MURABAYASHI Shun, MITAMURA Yoshinori

  IEICE technical report. ME and bio cybernetics ( The Institute of Electronics, Information and Communication Engineers )  105 ( 122 ) 9 - 12  2005.06

  　View Summary

  We present a new algorithm to detect Sleep Apnea easier from spectrum analysis of heart rate fluctuation. This algorithm aplied fitting sinusoid is a method of transformation from Respiratory Sinus Arrhythmia of R-R interval into respiration intervals. The respiration intervals calculated by this method were corresponded well with the subject of healthy and Sleep Apnea Syndrome. The proposed analytical method in this study showed the possibility of Sleep Apnea detection.

  CiNii

display all >>