水尾　圭祐

写真a

所属

医学部 法医学講座

職名

講師
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経歴 【 表示 ／ 非表示 】

  • 2008年04月
    -
    継続中

    札幌医科大学   助教・医学部

    助教・医学部

  • 2007年04月
    -
    2008年03月

    東京理科大学総合研究機構   ポストドクトラル研究員

    ポストドクトラル研究員

  • 2004年04月
    -
    2007年03月

    Temple University School of Medicine   Postdoctoral fellow

    Postdoctoral fellow

  • 2004年03月
    　
    　

    星薬科大学大学院薬学研究科博士課程後期修了   博士 (薬学) 取得

    博士 (薬学) 取得

  • 2001年04月
    -
    2004年03月

    星薬科大学大学院薬学研究科博士課程後期   大学院生

    大学院生

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所属学協会 【 表示 ／ 非表示 】

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    International Society for Biomedical Research on Alcoholism

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    日本法医学会

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    日本神経科学学会

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    日本アルコール・薬物医学会

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    Society for Neuroscience

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研究分野 【 表示 ／ 非表示 】

  • ライフサイエンス   薬系衛生、生物化学  

  • ライフサイエンス   法医学  

  • ライフサイエンス   薬理学  

  • ライフサイエンス   神経科学一般  

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研究キーワード 【 表示 ／ 非表示 】

  • アルコール

  • 薬物依存

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  • Maternal exposure to nanoparticulate titanium dioxide during the prenatal period alters gene expression related to brain development in the mouse

    Midori Shimizu, Hitoshi Tainaka, Taro Oba, Keisuke Mizuo, Masakazu Umezawa, Ken Takeda

    PARTICLE AND FIBRE TOXICOLOGY ( BIOMED CENTRAL LTD )  6   20  2009年07月  [査読有り]

    　概要を見る

    Background: Nanotechnology is developing rapidly throughout the world and the production of novel man-made nanoparticles is increasing, it is therefore of concern that nanomaterials have the potential to affect human health. The purpose of this study was to investigate the effects of maternal exposure to nano-sized anatase titanium dioxide (TiO2) on gene expression in the brain during the developmental period using cDNA microarray analysis combined with Gene Ontology (GO) and Medical Subject Headings (MeSH) terms information. Results: Analysis of gene expression using GO terms indicated that expression levels of genes associated with apoptosis were altered in the brain of newborn pups, and those associated with brain development were altered in early age. The genes associated with response to oxidative stress were changed in the brains of 2 and 3 weeks old mice. Changes of the expression of genes associated with neurotransmitters and psychiatric diseases were found using MeSH terms. Conclusion: Maternal exposure of mice to TiO2 nanoparticles may affect the expression of genes related to the development and function of the central nervous system.

    DOI PubMed

  • Effect of prenatal exposure to diesel exhaust on dopaminergic system in mice

    Satoshi Yokota, Keisuke Mizuo, Nozomu Moriya, Shigeru Oshio, Isamu Sugawara, Ken Takeda

    NEUROSCIENCE LETTERS ( ELSEVIER IRELAND LTD )  449 ( 1 ) 38 - 41  2009年01月  [査読有り]

    　概要を見る

    Diesel exhaust (DE) is composed of particles and gaseous compounds. It has been reported that DE causes pulmonary and cardiovascular disease. We have previously reported that fetal exposure to DE had deleterious effects to the reproductive system of mice offspring. However, there is still little known about the effects of prenatal exposure to DE to the central nervous system (CNS). In the present study, we found that prenatal exposure to DE induced reduction of locomotion, furthermore, dopamine (DA) turnover was significantly decreased in the striatum and nucleus accumbens. These results suggest that prenatal exposure to DE has an effect on the CNS. Hypolocomotion could be due to a decrease in DA turnover associated with DA nervous system abnormality. The present study provides the possibility that maternally inhaled DE might influence the development of central dopaminergic system and result in behavior disorder. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

    DOI PubMed

  • Changes in central dopaminergic systems and morphine reward by prenatal and neonatal exposure to bisphenol-A in mice: evidence for the importance of exposure period

    Minoru Narita, Kazuya Miyagawa, Keisuke Mizuo, Takuya Yoshida, Tsutomu Suzuki

    ADDICTION BIOLOGY ( BLACKWELL PUBLISHING )  12 ( 2 ) 167 - 172  2007年06月  [査読有り]

    　概要を見る

    Bisphenol-A has been extensively evaluated for toxicity in a variety of tests as the most common environmental endocrine disruptors. In a previous study, we reported that exposure to bisphenol-A affects the development of the central dopaminergic system in the mouse limbic area. The present study was undertaken to investigate the relationship between the developmental toxicity of bisphenol-A and its exposure period. The exposure to bisphenol-A during either organogenesis or lactation, but not implantation and parturition, significantly enhanced the morphine-induced hyperlocomotion and rewarding effects. Furthermore, exposure to bisphenol-A during either organogenesis or lactation also produced an up-regulation of dopamine receptor function to activate G-protein in the mouse limbic forebrain. These results indicate that both organogenesis and lactation are more sensitive to the bisphenol-A-induced developmental neuronal toxicology than any other periods. In conclusion, the present data suggest that the organogenesis and lactation are the most important period to cause the alternation of dopaminergic system by bisphenol-A exposure in the mouse.

    DOI PubMed

  • Smooth muscle-associated protein 8: Distribution and biological activity in the rat brain

    G. Cristina Brailoiu, Siok L. Dun, Keisuke Mizuo, Eugen Brailoiu, Jun Yang, Jaw Kang Chang, Nae J. Dun

    JOURNAL OF NEUROSCIENCE RESEARCH ( WILEY-LISS )  85 ( 8 ) 1789 - 1796  2007年06月  [査読有り]

    　概要を見る

    With the use of an antiserum directed against the human smooth muscle-associated protein 8 (SMAP8) fragment SMAP898-138, Western blot and immunohistochemical studies revealed SMAP8 expression in the rat brain. A band with a molecular size of about 45 kDa was detected in tissues from the rat hypothalamus and a weaker band from the cortex. SMAP8 immunoreactivity (irSMAP8) was detected in neurons of the hypothalamic paraventricular, supraoptic, and supraoptic retrochiasmatic nuclei; a few irSMAP8 cells were scattered in the zona incerta as well as the cerebral cortex. Immunoreactive cell processes were detected mostly in the internal layer of the median eminence. Double labeling the hypothalamic sections with SMAP8 and vasopressin (VP) or oxytocin (OT) antiserum revealed that a population of VP- and OT-immunoreactive neurons expressed irSMAP8. The biological activity of SMAP8 in rat central neurons was assessed by the calcium microfluorimetric Fura-2 method. SMAP8 (100 nM) elevated cytosolic calcium concentrations [Ca2+](i) in a population of dissociated and cultured rat hypothalamic neurons; the response was eliminated in Ca2+-free saline. This is the first evidence of irSMAP8 in a population of MOT-containing hypothalamic neurons in the rat, and the peptide is biologically active in hypothalamic neurons, as evidenced by-mobilization of extracellular Ca2+. (c) 2007 Wiley-Liss, Inc.

    DOI PubMed

  • Distribution and characterization of estrogen receptor G protein-coupled receptor 30 in the rat central nervous system

    Eugen Brailoiu, Siok L. Dun, G. Cristina Brailoiu, Keisuke Mizuo, Larry A. Sklar, Tudor I. Oprea, Eric R. Prossnitz, Nae J. Dun

    JOURNAL OF ENDOCRINOLOGY ( SOC ENDOCRINOLOGY )  193 ( 2 ) 311 - 321  2007年05月  [査読有り]

    　概要を見る

    The G protein-coupled receptor 30 (GPR 30) has been identified as the non-genomic estrogen receptor, and G-1, the specific ligand for GPR30. With the use of a polyclonal antiserum directed against the human C-terminus of GPR30, immunohistochemical studies revealed GPR30-immuno-reactivity (irGPP30) in the brain of adult male and nonpregnant female rats. A high density of irGPR30 was noted in the Islands of Calleja and striaturn. In the hypothalamus, irGPR30 was detected in the paraventricular nucleus and supraoptic nucleus. The anterior and posterior pituitary contained numerous irGPR30 cells and terminal-like endings. Cells in the hippocampal formation as well as the substantia nigra were irGPR30. In the brainstem, irGPR30 cells were noted in the area postrema, nucleus of the solitary tract, and dorsal motor nucleus of the vagus; a cluster of cells were prominently labeled in the nucleus ambiguus. Tissue sections processed with pre-immune serum showed no irGPR30, affirming the specificity of the antiserum. G-1 (100 nM) caused a large increase of intracellular calcium concentrations [Ca2+](i) in dissociated and cultured rat hypothalamic neurons, as assessed by microfluorometric Fura-2 imaging. The calcium response to a second application of G-1 showed a marked homologous desensitization. Our result shows a high expression of irGPR30 in the hypothalamic-pituitary axis, hippocampal formation, and brainstem autonomic nuclei; and the activation of GPR30 by G-1 is associated with a mobilization of calcium in dissociated and cultured rat hypothalamic neurons.

    DOI PubMed

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受賞 【 表示 ／ 非表示 】

  • 奨励賞

    2003年   ニコチン・薬物依存研究フォーラム  

    受賞者： 水尾圭祐

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共同研究・競争的資金等の研究課題 【 表示 ／ 非表示 】

  • アルコール等濫用薬物のフラッシュバック現象の分子機構―マイクロRNAの役割

    若手研究(B)

    研究期間:

    2009年
    　
    　
     

    水尾 圭祐

    　研究概要を見る

    アルコールをはじめとする濫用薬物は長期の使用により依存を形成し、一度依存が形成されてしまうと断薬期間があっても再度の摂取により再び依存に陥るフラッシュバック現象を引き起こすことが知られている。また、飲酒者の死亡例については血中アルコール濃度とその中枢神経系に及ぼす影響を考慮しなければならず、アルコールの作用発現機序を考えることは重要である。本研究では、エタノール単回投与によってマイクロRNAが12時間以上におよぶ持続的な発現増加することを明らかにした。また、エタノール投与後の脳内においてヒストンアセチル化の増加が認められることを見いだし、この現象がどの脳部位で生じているのかを明らかにした。さらに、依存モデル動物の脳内においてマイクロRNAの上昇が認められることを明らかにし、エタノールの依存にマイクロRNAが関与する可能性を示唆した。

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講演・口頭発表等 【 表示 ／ 非表示 】

  • Prenatal exposure to titanium dioxide nanoparticles alters brain monoamine concentration in mice

    Keisuke Mizuo, Yuta Takahashi, Ken Takeda

    Neuroscience 2009  

    発表年月： 2009年10月

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