Organization
School of Medicine Department of Hygiene Associate Professor
Title
Associate Professor
External link
URUSHIBARA Noriko
Degree
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PhD in Science (Life Science) ( 1999.3 Hokkaido University )
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MSc in Pharmaceutical Sciences ( 1990.3 Hokkaido University )
Research Interests
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細胞骨格
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系統
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環境因子
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platelet
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遺伝子配列
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全遺伝子分節
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新変異型
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アジア
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血小板
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Arf-GAP
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黄色ブドウ球菌
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ミャンマー
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環境応答
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リアソートメント
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actin
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ロタウイルス
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ストレス
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ゲノム
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遺伝子再集合体
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国際研究者交流
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スタフィロコアグラーゼ
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市中感染
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全ゲノム
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シグナル伝達
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SCCmec
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分子疫学
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PVL
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ACME
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遺伝子型
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MRSA
Research Areas
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Life Science / Medical management and medical sociology
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Life Science / Hygiene and public health (laboratory)
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Life Science / Hematology and medical oncology
Research History
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Sapporo Medical University Department of Hygiene Associate Professor
2017.7
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Sapporo Medical University School of Medicine Lecturer
2012
Papers
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Prevalence and genetic characteristics of Staphylococcus capitis proto-NRCS-A clone among colonizing isolates from healthy individuals in Japan.
Hirose, Mina; Aung, Meiji Soe; Hirose, Yukito; Nakanishi, Yasuhiro; Kato, Taisei; Ichimura, Akihiro; Kurashige, Yoshihito; Sakakibara, Sayaka; Minowa-Suzuki, Erika; Saitoh, Masato; Urushibara, Noriko; Kawaguchiya, Mitsuyo; Ohashi, Nobuhide; Kobayashi, Nobumichi
J Infect Public Health 19 ( 2 ) 2026
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Meiji Soe Aung, Noriko Urushibara, Mitsuyo Kawaguchiya, Nobuhide Ohashi, Thida San, Win Kalayar Kyaw, Mina Hirose, Masako Osada, Masahiko Ito, Nobumichi Kobayashi
Acta Microbiologica Hellenica 2025.12
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Molecular characterization and antimicrobial resistance of Streptococcus pneumoniae isolated from adult patients with invasive pneumococcal disease in northern Japan, 2017-2023. International journal
Mitsuyo Kawaguchiya, Meiji Soe Aung, Noriko Urushibara, Nobuhide Ohashi, Nobuyuki Kobayashi, Kenji Kudo, Masahiko Ito, Nobumichi Kobayashi
IJID regions 16 100693 - 100693 2025.9
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Epidemiological features of scrub typhus and molecular characteristics of Orientia tsutsugamushi in north-central Bangladesh
Sultana Shabnam Nila, Meiji Soe Aung, Nazia Haque, Shyamal Kumar Paul, Syeda Anjuman Nasreen, Sangjukta Roy, Salma Ahmed, Jobyda Khanam, Mohammad Abdul Awal Bin Sayeed, Shashwata Paul, Noriko Urushibara, Mitsuyo Kawaguchiya, Nobuhide Ohashi, Nobumichi Kobayashi
IJID REGIONS 14 2025.3
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Molecular characterization of methicillin-susceptible/resistant Staphylococcus aureus from bloodstream infections in northern Japan: The dominance of CC1-MRSA-IV, the emergence of human-associated ST398 and livestock-associated CC20 and CC97 MSSA.
Aung, Meiji Soe; Osada, Masako; Urushibara, Noriko; Kawaguchiya, Mitsuyo; Ohashi, Nobuhide; Hirose, Mina; Ito, Masahiko; Yamada, Kazuki; Tada, Kousuke; Kobayashi, Nobumichi
J Glob Antimicrob Resist 41 2025
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Prevalence and antimicrobial resistance of three clones (ST1223, ST2198, ST2250) of Staphylococcus argenteus clinical isolates in northern Japan.
Aung, Meiji Soe; Urushibara, Noriko; Kawaguchiya, Mitsuyo; Ohashi, Nobuhide; Hirose, Mina; Kudo, Kenji; Ito, Masahiko; Kobayashi, Nobumichi
J Glob Antimicrob Resist 40 2025
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Serotype distribution and antimicrobial resistance of<i> Streptococcus</i><i> pneumoniae</i> isolated from children in Japan, 2023
Mitsuyo Kawaguchiya, Noriko Urushibara, Meiji Soe Aung, Nobuhide Ohashi, Sho Tsutida, Kayo Kurashita, Masahiko Ito, Nobumichi Kobayashi
NEW MICROBES AND NEW INFECTIONS 62 2024.12
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Genetic characterization of methicillin-resistant / susceptible Staphylococcus aureus (MRSA/MSSA) and Staphylococcus argenteus clinical isolates in Bangladesh: Dominance of ST6-MRSA-IV/t304 and detection of<i> cfr</i><i> /fexA</i> in ST8-MSSA/t008
Sangjukta Roy, Meiji Soe Aung, Shyamal Kumar Paul, Syeda Anjuman Nasreen, Nazia Haque, Rummana Mazid, Md. Shahed Khan, Tridip Kanti Barman, Parvez Arafa, Fardousi Akter Sathi, Sultana Shabnam Nila, Afsana Jahan, Noriko Urushibara, Mitsuyo Kawaguchiya, Nobuhide Ohashi, Nobumichi Kobayashi
IJID REGIONS 10 132 - 139 2024.3
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Molecular Epidemiological Characterization of Methicillin-Resistant Staphylococcus aureus from Bloodstream Infections in Northern Japan: Increasing Trend of CC1 and Identification of ST8-SCCmec IVa USA300-Like Isolate Lacking Arginine Catabolic Mobile Element.
Aung, Meiji Soe; Urushibara, Noriko; Kawaguchiya, Mitsuyo; Ohashi, Nobuhide; Hirose, Mina; Kimura, Yuuki; Kudo, Kenji; Ito, Masahiko; Kobayashi, Nobumichi
Microb Drug Resist 30 ( 2 ) 2024
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Prevalence, clonal diversity, and antimicrobial resistance of hypervirulent Klebsiella pneumoniae and Klebsiella variicola clinical isolates in northern Japan
Norifumi Matsuda, Meiji Soe Aung, Noriko Urushibara, Mitsuyo Kawaguchiya, Nobuhide Ohashi, Kenji Taniguchi, Kenji Kudo, Masahiko Ito, Nobumichi Kobayashi
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE 35 11 - 18 2023.12
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Spread of multidrug resistance in non-PCV13/PCV20 serotypes of<i> Streptococcus</i><i> pneumoniae:</i> A cross-sectional study ten years after the introduction of pneumococcal conjugate vaccine in Japan
Mitsuyo Kawaguchiya, Noriko Urushibara, Meiji Soe Aung, Nobuhide Ohashi, Rika Takamatsu, Sho Tsutida, Masahiko Ito, Nobumichi Kobayashi
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION 56 ( 6 ) 1288 - 1292 2023.12
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Meiji Soe Aung, Noriko Urushibara, Mitsuyo Kawaguchiya, Nobuhide Ohashi, Mina Hirose, Kenji Kudo, Naoyuki Tsukamoto, Masahiko Ito, Nobumichi Kobayashi
Antibiotics 12 ( 1 ) 2023.1
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Detecting the performance of methicillin-resistant Staphylococcus aureus by a molecular diagnostic assay in positive blood culture: Influence of coexistence of mecA-positive bacteria and diversity in orfX-SCCmec junction region in methicillin-susceptible S. aureus.
Saeki, Masachika; Nirasawa, Shinya; Aung, Meiji Soe; Ono, Mayumi; Urushibara, Noriko; Kobayashi, Nobumichi; Takahashi, Satoshi
J Infect Chemother 29 ( 9 ) 2023
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Genome analysis of an SCCmec element in ST9-MRSA from Myanmar with a unique mec gene complex and two ccr gene complexes (ccrA1B1 and ccrA5B7).
Urushibara, Noriko; Aung, Meiji Soe; Kawaguchiya, Mitsuyo; Ohashi, Nobuhide; Kobayashi, Nobumichi
J Antimicrob Chemother 78 ( 7 ) 2023
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Molecular Epidemiological Characterization of Staphylococcus aureus and Staphylococcus argenteus Clinical Isolates from a National Tertiary Care Hospital in Myanmar: Co-Isolation of Multiple Clones and Identification of Novel Staphylocoagulase Genotype.
Kyaw, Win Kalayar; Aung, Meiji Soe; San, Thida; Maw, Win Win; Mu, Khin Khin; Mon, Win Lei Yee; Than, Mie Mie; San, Nilar; Than, Cho Cho; Urushibara, Noriko; Kobayashi, Nobumichi
Microb Drug Resist 29 ( 4 ) 2023
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Mina Hirose, Meiji Soe Aung, Yusuke Fujita, Taisei Kato, Yukito Hirose, Shoko Yahata, Atsushi Fukuda, Masato Saitoh, Noriko Urushibara, Nobumichi Kobayashi
Pathogens 2022.7
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Masako Osada, Meiji Soe Aung, Noriko Urushibara, Mitsuyo Kawaguchiya, Nobuhide Ohashi, Mina Hirose, Nobumichi Kobayashi
Pathogens 11 ( 4 ) 2022.4
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Clonal Diversity and Molecular Characteristics of Methicillin-Susceptible and -Resistant Staphylococcus aureus from Pediatric Patients in Myanmar.
Aung, Meiji Soe; San, Thida; Urushibara, Noriko; San, Nilar; Hlaing, Myat Su; Soe, Pan Ei; Htut, Wut Hmone Win; Moe, Ingin; Mon, Win Lei Yi; Chan, Zar Chi Nyein; Kobayashi, Nobumichi
Microb Drug Resist 28 ( 2 ) 2022
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Clonal diversity of Clostridium perfringens human clinical isolates with various toxin gene profiles based on multilocus sequence typing and alpha-toxin (PLC) typing
Meiji Soe Aung, Asami Matsuda, Noriko Urushibara, Mitsuyo Kawaguchiya, Nobuhide Ohashi, Norifumi Matsuda, Mayumi Nakamura, Masahiko Ito, Satoshi Habadera, Akinori Matsumoto, Mina Hirose, Nobumichi Kobayashi
ANAEROBE 72 2021.12
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Mina Hirose, Meiji Soe Aung, Atsushi Fukuda, Shoko Yahata, Yusuke Fujita, Masato Saitoh, Yukito Hirose, Noriko Urushibara, Nobumichi Kobayashi
Antibiotics 2021.10
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Meiji Soe Aung, Noriko Urushibara, Mitsuyo Kawaguchiya, Mina Hirose, Miyo Ike, Masahiko Ito, Nobumichi Kobayashi
Pathogens 10 ( 2 ) 2021.2
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Clonal diversity of methicillin-resistant Staphylococcus aureus (MRSA) from bloodstream infections in northern Japan: Identification of spermidine N-acetyltransferase gene (speG) in staphylococcal cassette chromosomes (SCCs) associated with type II and IV SCCmec.
Aung, Meiji Soe; Urushibara, Noriko; Kawaguchiya, Mitsuyo; Hirose, Mina; Ito, Masahiko; Habadera, Satoshi; Kobayashi, Nobumichi
J Glob Antimicrob Resist 24 2021
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Sangjukta Roy, Meiji Soe Aung, Shyamal Kumar Paul, Salma Ahmed, Nazia Haque, Emily Rahman Khan, Tridip Kanti Barman, Arup Islam, Sahida Abedin, Chand Sultana, Anindita Paul, Muhammad Akram Hossain, Noriko Urushibara, Mitsuyo Kawaguchiya, Ayako Sumi, Nobumichi Kobayashi
Microorganisms 2020.8
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Meiji Soe Aung, Noriko Urushibara, Mitsuyo Kawaguchiya, Masahiko Ito, Satoshi Habadera, Nobumichi Kobayashi
Toxins 12 ( 5 ) 2020.5
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Dianelys Quiñones, Meiji Soe Aung, Yenisel Carmona, María Karla González, Niurka Pereda, Mercedes Hidalgo, Mayrelis Rivero, Arnaldo Zayas, Rosa del Campo, Noriko Urushibara, Nobumichi Kobayashi
Pathogens 2020.1
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Pacemaker-associated infection caused by ST81/SCCmec IV methicillin-resistant, vancomycin-intermediate Staphylococcus aureus in Japan.
Sakurada, M.; Sumi, H.; Kaji, K.; Kobayashi, N.; Sakai, Y.; Aung, M. S.; Urushibara, N.; Kobayashi, N.
New Microbes New Infect 35 2020
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Novel staphylococcal cassette chromosome mec (SCCmec) type XIV (5A) and a truncated SCCmec element in SCC composite islands carrying speG in ST5 MRSA in Japan.
Urushibara, Noriko; Aung, Meiji Soe; Kawaguchiya, Mitsuyo; Kobayashi, Nobumichi
J Antimicrob Chemother 75 ( 1 ) 2020
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Molecular Characterization of Methicillin-Susceptible and -Resistant Staphylococcus aureus Harboring Panton-Valentine Leukocidin-Encoding Bacteriophages in a Tertiary Care Hospital in Myanmar.
Aung, Meiji Soe; San, Thida; Urushibara, Noriko; San, Nilar; Oo, Win Mar; Soe, Pan Ei; Kyaw, Yamin; Ko, Phyo May; Thu, Pyae Phyo; Hlaing, Myat Su; Kawaguchiya, Mitsuyo; Kobayashi, Nobumichi
Microb Drug Resist 26 ( 4 ) 2020
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Mitsuyo Kawaguchiya, Noriko Urushibara, Meiji Soe Aung, Masaaki Shinagawa, Satoshi Takahashi, Nobumichi Kobayashi
Pathogens 2019.9
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Asami Matsuda, Meiji Soe Aung, Noriko Urushibara, Mitsuyo Kawaguchiya, Ayako Sumi, Mayumi Nakamura, Yuka Horino, Masahiko Ito, Satoshi Habadera, Nobumichi Kobayashi
Toxins 11 ( 6 ) 2019.6
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Molecular Epidemiological Characterization of Staphylococcus argenteus Clinical Isolates in Japan: Identification of Three Clones (ST1223, ST2198, and ST2550) and a Novel Staphylocoagulase Genotype XV.
Aung, Meiji Soe; Urushibara, Noriko; Kawaguchiya, Mitsuyo; Sumi, Ayako; Takahashi, Seika; Ike, Miyo; Ito, Masahiko; Habadera, Satoshi; Kobayashi, Nobumichi
Microorganisms 7 ( 10 ) 2019
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Clonal Diversity and Genetic Characteristics of Methicillin-Resistant Staphylococcus aureus Isolates from a Tertiary Care Hospital in Japan.
Aung, Meiji Soe; Urushibara, Noriko; Kawaguchiya, Mitsuyo; Sumi, Ayako; Shinagawa, Masaaki; Takahashi, Satoshi; Kobayashi, Nobumichi
Microb Drug Resist 25 ( 8 ) 2019
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Molecular characterization of Staphylococcus argenteus in Myanmar: identification of novel genotypes/clusters in staphylocoagulase, protein A, alpha-haemolysin and other virulence factors.
Aung, Meiji Soe; San, Thida; San, Nilar; Oo, Win Mar; Ko, Phyoe May; Thet, Khin Thet; Urushibara, Noriko; Kawaguchiya, Mitsuyo; Sumi, Ayako; Kobayashi, Nobumichi
J Med Microbiol 68 ( 1 ) 2019
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Contribution of Type II Topoisomerase Mutations to Fluoroquinolone Resistance in Enterococcus faecium from Japanese Clinical Setting.
Urushibara, Noriko; Suzaki, Keisuke; Kawaguchiya, Mitsuyo; Aung, Meiji Soe; Shinagawa, Masaaki; Takahashi, Satoshi; Kobayashi, Nobumichi
Microb Drug Resist 24 ( 1 ) 2018
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Genetic characteristics of VanA-type vancomycin-resistant Enterococcus faecalis and Enterococcus faecium in Cuba.
Quiテアones, D.; Aung, M. S.; Sousa Martins, J. P.; Urushibara, N.; Kobayashi, N.
New Microbes New Infect 21 2018
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Noriko Urushibara
Toxins 9 ( 8 ) 2017.8
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Molecular Characterization of Methicillin-Resistant Staphylococcus aureus from Outpatients in Northern Japan: Increasing Tendency of ST5/ST764 MRSA-IIa with Arginine Catabolic Mobile Element.
Aung, Meiji Soe; Kawaguchiya, Mitsuyo; Urushibara, Noriko; Sumi, Ayako; Ito, Masahiko; Kudo, Kenji; Morimoto, Shigeo; Hosoya, Shino; Kobayashi, Nobumichi
Microb Drug Resist 23 ( 5 ) 2017
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グローバル感染症と薬剤耐性菌対策の動向～最近のMRSA、薬剤耐性肺炎球菌の特徴について～
小林 宣道, 川口谷 充代, 漆原 範子
産業医学ジャーナル 40 52 - 57 2017
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Collaborative Research on Puerperal Infections in Bangladesh
KOBAYASHI Nobumichi, AHMED Salma, SUMI Ayako, URUSHIBARA Noriko, KAWAGUCHIYA Mitsuyo, AUNG Meiji Soe
Nippon Eiseigaku Zasshi (Japanese Journal of Hygiene) 72 ( 2 ) 106 - 111 2017
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Novel Structures and Temporal Changes of Arginine Catabolic Mobile Elements in Methicillin-Resistant Staphylococcus aureus Genotypes ST5-MRSA-II and ST764-MRSA-II in Japan.
Urushibara, Noriko; Kawaguchiya, Mitsuyo; Onishi, Mayumi; Mise, Keiji; Aung, Meiji Soe; Kobayashi, Nobumichi
Antimicrob Agents Chemother 60 ( 5 ) 2016
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Detection of ST772 Panton-Valentine leukocidin-positive methicillin-resistant Staphylococcus aureus (Bengal Bay clone) and ST22 S.ﾂaureus isolates with a genetic variant of elastin binding protein in Nepal.
Pokhrel, R. H.; Aung, M. S.; Thapa, B.; Chaudhary, R.; Mishra, S. K.; Kawaguchiya, M.; Urushibara, N.; Kobayashi, N.
New Microbes New Infect 11 2016
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Drug resistance and genetic characteristics of clinical isolates of staphylococci in Myanmar: high prevalence of PVL among methicillin-susceptible Staphylococcus aureus belonging to various sequence types.
Aung, M. S.; Zi, H.; Nwe, K. M.; Maw, W. W.; Aung, M. T.; Min, W. W.; Nyein, N.; Kawaguchiya, M.; Urushibara, N.; Sumi, A.; Kobayashi, N.
New Microbes New Infect 10 2016
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Rickettsia felis Infection among Humans, Bangladesh, 2012-2013.
Ferdouse, Faria; Hossain, Muhammad Akram; Paul, Shyamal Kumar; Ahmed, Salma; Mahmud, Md Chand; Ahmed, Rajib; Haque, A. K. M. Fazlul; Khan, M. Nur-A.-Alam; Ghosh, Souvik; Urushibara, Noriko; Kobayashi, Nobumichi
Emerg Infect Dis 21 ( 8 ) 2015
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Detection and genetic characterization of PVL-positive ST8-MRSA-IVa and exfoliative toxin D-positive European CA-MRSA-Like ST1931 (CC80) MRSA-IVa strains in Bangladesh.
Paul, Shyamal Kumar; Ghosh, Souvik; Kawaguchiya, Mitsuyo; Urushibara, Noriko; Hossain, Mohammad Akram; Ahmed, Salma; Mahmud, Chand; Jilani, Md Shariful Alam; Haq, Jalaluddin Ashraful; Ahmed, Abdullah Akhtar; Kobayashi, Nobumichi
Microb Drug Resist 20 ( 4 ) 2014
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Characterization of Enterococcus faecium with macrolide resistance and reduced susceptibility to quinupristin/dalfopristin in a Japanese hospital: detection of extensive diversity in erm(B)-regulator regions.
Isogai, Nayuta; Urushibara, Noriko; Kawaguchiya, Mitsuyo; Ghosh, Souvik; Suzaki, Keisuke; Watanabe, Naoki; Quiテアones, Dianelys; Kobayashi, Nobumichi
Microb Drug Resist 19 ( 4 ) 2013
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Characterization of PVL/ACME-positive methicillin-resistant Staphylococcus aureus (genotypes ST8-MRSA-IV and ST5-MRSA-II) isolated from a university hospital in Japan.
Kawaguchiya, Mitsuyo; Urushibara, Noriko; Yamamoto, Dai; Yamashita, Toshiharu; Shinagawa, Masaaki; Watanabe, Naoki; Kobayashi, Nobumichi
Microb Drug Resist 19 ( 1 ) 2013
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Prevalence and genetic diversity of arginine catabolic mobile element (ACME) in clinical isolates of coagulase-negative staphylococci: identification of ACME type I variants in Staphylococcus epidermidis.
Onishi, Mayumi; Urushibara, Noriko; Kawaguchiya, Mitsuyo; Ghosh, Souvik; Shinagawa, Masaaki; Watanabe, Naoki; Kobayashi, Nobumichi
Infect Genet Evol 20 2013
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Genetic diversity of emerging Panton-Valentine leukocidine/arginine catabolic mobile element (ACME)-positive ST8 SCCmec-IVa meticillin-resistant Staphylococcus aureus (MRSA) strains and ACME-positive CC5 (ST5/ST764) MRSA strains in Northern Japan.
Kawaguchiya, Mitsuyo; Urushibara, Noriko; Ghosh, Souvik; Kuwahara, Osamu; Morimoto, Shigeo; Ito, Masahiko; Kudo, Kenji; Kobayashi, Nobumichi
J Med Microbiol 62 2013
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Population deviation of piggery-associated methicillin-resistant Staphylococcus aureus based on mec-associated direct repeat unit analysis.
Wan, Min Tao; Lauderdale, Tsai Ling; Kobayashi, Nobumichi; Urushibara, Noriko; Chou, Chin Cheng
Infect Genet Evol 16 2013
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Whole-genomic analysis of a human G1P[9] rotavirus strain reveals intergenogroup-reassortment events Reviewed
Souvik Ghosh, Tsuzumi Shintani, Noriko Urushibara, Koki Taniguchi, Nobumichi Kobayashi
JOURNAL OF GENERAL VIROLOGY 93 1700 - 1705 2012.8
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Whole genomic analysis reveals the porcine origin of human G9P[19] rotavirus strains Mc323 and Mc345 Reviewed
Souvik Ghosh, Noriko Urushibara, Koki Taniguchi, Nobumichi Kobayashi
INFECTION GENETICS AND EVOLUTION 12 ( 2 ) 471 - 477 2012.3
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Two novel arginine catabolic mobile elements and staphylococcal chromosome cassette mec composite islands in community-acquired methicillin-resistant Staphylococcus aureus genotypes ST5-MRSA-V and ST5-MRSA-II.
Urushibara, Noriko; Kawaguchiya, Mitsuyo; Kobayashi, Nobumichi
J Antimicrob Chemother 67 ( 8 ) 2012
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Whole-genome analysis reveals the complex evolutionary dynamics of Kenyan G2P[4] human rotavirus strains Reviewed
Souvik Ghosh, Noriaki Adachi, Zipporah Gatheru, James Nyangao, Dai Yamamoto, Masaho Ishino, Noriko Urushibara, Nobumichi Kobayashi
JOURNAL OF GENERAL VIROLOGY 92 2201 - 2208 2011.9
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Analysis of Staphylococcal cassette chromosome mec in Staphylococcus haemolyticus and Staphylococcus sciuri: identification of a novel ccr gene complex with a newly identified ccrA allotype (ccrA7).
Urushibara, Noriko; Paul, Shyamal Kumar; Hossain, Mohammad Akram; Kawaguchiya, Mitsuyo; Kobayashi, Nobumichi
Microb Drug Resist 17 ( 2 ) 2011
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Virulence factors and genetic characteristics of methicillin-resistant and -susceptible Staphylococcus aureus isolates in Myanmar.
Aung, Meiji Soe; Urushibara, Noriko; Kawaguchiya, Mitsuyo; Aung, Tin Sabai; Mya, San; San, Thidar; Nwe, Khin Myat; Kobayashi, Nobumichi
Microb Drug Resist 17 ( 4 ) 2011
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Molecular characteristics of community-acquired methicillin-resistant Staphylococcus aureus in Hokkaido, northern main island of Japan: identification of sequence types 6 and 59 Panton-Valentine leucocidin-positive community-acquired methicillin-resistant Staphylococcus aureus.
Kawaguchiya, Mitsuyo; Urushibara, Noriko; Kuwahara, Osamu; Ito, Masahiko; Mise, Keiji; Kobayashi, Nobumichi
Microb Drug Resist 17 ( 2 ) 2011
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Identification of staphylocoagulase genotypes I-X and discrimination of type IV and V subtypes by multiplex PCR assay for clinical isolates of Staphylococcus aureus.
Hirose, Mina; Kobayashi, Nobumichi; Ghosh, Souvik; Paul, Shyamal Kumar; Shen, Tzuhsiang; Urushibara, Noriko; Kawaguchiya, Mitsuyo; Shinagawa, Masaaki; Watanabe, Naoki
Jpn J Infect Dis 63 ( 4 ) 2010
MISC
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バングラデシュにおけるコアグラーゼ陽性ブドウ球菌臨床分離株の分子疫学的解析
小林宣道, アウン メイジソウ, ロイ サンジュクタ, 漆原範子, 川口谷充代, 大橋伸英
日本衛生学雑誌(Web) 80 ( Supplement ) 2025
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ミャンマー由来ST9型黄色ブドウ球菌の全ゲノム解析:分子疫学的特徴と系統進化
漆原範子, アウン メイジソウ, 川口谷充代, 大橋伸英, 大橋伸英
日本衛生学雑誌(Web) 80 ( Supplement ) 2025
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Secular changes in the serotype distribution of pediatric pneumococci in Hokkaido, 2011-2023
川口谷充代, 漆原範子, AUNG Meiji Soe, 伊藤政彦, 小林宣道
日本細菌学雑誌(Web) 79 ( 2 ) 2024
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Molecular epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) from bloodstream infections (BSIs) in Hokkaido, Northern Japan
AUNG Meiji Soe, 漆原範子, 川口谷充代, 大橋伸英, 荒木蕗羽, 松原加奈, 伊藤政彦, 小林宣道
日本ブドウ球菌研究会プログラム・抄録集(Web) 68th 2024
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Current trends in serotype distribution of Streptococcus pneumoniae isolated from children in 2023
川口谷充代, 漆原範子, AUNG Meiji Soe, 大橋伸英, 木村優希, 堀野裕香, 伊藤政彦, 小林宣道
日本細菌学雑誌(Web) 79 ( 2 ) 2024
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Recent trend of serotypes and antimicrobial resistance of non-invasive <i>Streptococcus pneumoniae</i> in Hokkaido, Japan
Mitsuyo Kawaguchiya, Noriko Urushibara, Meiji Soe Aung, Shigeo Morimoto, Masahiko Ito, Kenji Kudo
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS 50 S218 - S218 2017.11
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産業医に役立つ最新の研究報告 グローバル感染症と薬剤耐性菌対策の動向 : 最近のMRSA,薬剤耐性肺炎球菌の特徴について
小林 宣道, 川口谷 充代, 漆原 範子, メイジ ソウ アウン
産業医学ジャーナル = Occupational health journal 40 ( 3 ) 52 - 57 2017.5
Research Projects
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A Comprehensive Characterization of Clinical Isolates of Staphylococcal Small Colony Variant: the Use of Omics Approach
Grant number：24K13443 2024.4 - 2027.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Grant amount：\4550000 （ Direct Cost: \3500000 、 Indirect Cost：\1050000 ）
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Molecular epidemiology and comprehensive exploration of novel virulence factors and their action mechanisms of hypervirulent Staphylococcus in Asia
Grant number：23KK0171 2023.9 - 2027.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Fund for the Promotion of Joint International Research (International Collaborative Research)
Grant amount：\21060000 （ Direct Cost: \16200000 、 Indirect Cost：\4860000 ）
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ワンヘルスに基づいた生活環境由来細菌の疫学解析と全ゲノム解析
Grant number：21K10417 2021.4 - 2024.3
日本学術振興会 科学研究費助成事業 基盤研究(C)
漆原 範子, アウン メイジソウ
Grant amount：\3770000 （ Direct Cost: \2900000 、 Indirect Cost：\870000 ）
薬剤耐性対策には動物，食品，環境等を含めた分野横断的に取組むこと，すなわちワンヘルスアプローチが重要である。本研究の目的は，生活環境における感染起因菌の分布と薬剤耐性を調査し，ゲノム配列に基づいた比較解析・系統解析を通して耐性遺伝子の進化，及び伝播の様態を考察し，耐性菌の蔓延対策に資することである。
2021 年度は食肉由来の菌体の収集及び解析を中心に行った。札幌近郊の小売店にて購入された鶏肉及び豚由来の 127 株のブドウ球菌属細菌及び Mammaliicoccus 属細菌について 16S rRNA の配列に基づいた菌種の決定および，微量希釈法を用いた薬剤感受性を調査した。菌種をひとつに絞り込めない菌体も多かったため，遺伝系統の近い数種をまとめた species group（Becker k. et al, Clin Microbiol Rev. 2014）として同定した。
鶏肉由来では Cohnii-Nepalensis，Simulans に属するブドウ球菌細菌が多く，豚肉由来菌体では Saprophytics，Warneri が多かった。黄色ブドウ球菌は全体の 4% であった。薬剤感受性試験では，マクロライド系及びスルフォンアミド系の抗生剤に対する感受性が低下している菌体の検出頻度が高かった。マクロライド系抗生剤は家畜の飼料に添加され，スルフォンアミド系抗生剤は治療に用いられることが多い。それらの使用を通じて耐性菌が発生した可能性が考えられた。
Mammaliicoccus 属はブドウ球菌属細菌に共通のメチシリン耐性遺伝子 mecA の起源を染色体ゲノム上に持ち，mecA 遺伝子（群）の進化を考える上で重要である。13 株検出され，5 株が mecA 陽性であった。その中で 3 株は M. fleurettii で，mecA 遺伝子群の構造を決定した。 -
Molecular epidemiological study of novel species and emerging drug-resistant staphylococcus in Japan and Asia, and basic construction of their infection control
Grant number：20H03933 2020.4 - 2024.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Grant amount：\17680000 （ Direct Cost: \13600000 、 Indirect Cost：\4080000 ）
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蛋白結合型ワクチン導入後に分離された 無莢膜型肺炎球菌の薬剤耐性と分子疫学的特徴
Grant number：19K10603 2019.4 - 2023.3
日本学術振興会 科学研究費助成事業 基盤研究(C)
川口谷 充代, 小林 宣道, 漆原 範子
Grant amount：\4290000 （ Direct Cost: \3300000 、 Indirect Cost：\990000 ）
本研究の目的は蛋白結合型ワクチンの導入後における肺炎球菌の継続的な分子疫学調査に加え、莢膜を持たない無莢膜型肺炎球菌（NESp: nonencapsulated S. pneumoniae）の分子疫学的・遺伝学的特徴および薬剤耐性を明らかにすることである。本年度は、これまで継続して行なってきた分子疫学的研究の中で得られた遺伝学的知見を考察し、全無莢膜型肺炎球菌株に対して、β-ラクタム耐性に関与するペニシリン（PEN）結合蛋白（PBP）１a、PBP2b、PBP2xのトランスペプチダーゼ（TP）ドメインにおけるアミノ酸変異を分子遺伝学的手法で詳細に解析を行った。解析の結果、PBP1aでは370STMK373のT371S変異（76.0％）と428SRNVP432のP432T変異（71.8％）、TSQF574-577NTGYへのアミノ酸置換（76.0％）が高頻度で見られ、その80.4-81.5％がPEN非感受性株であった。 PBP2bでは442SSNT445のT445A変異（81.7％）が、PBP2xにおいては337STMK340のT338A変異（77.5％）と546LKSGT550のL546V変異（91.5％）が多く検出された。一方、394HSSN397においてH394L変異が見られた多く（83.3％）はPEN感受性であった。TPドメインには様々なパターン、多数の変異が存在していることが特にPBP2xにおいて確認され、NESpにおけるPBP変異には多様性があることを明らかにした。
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Molecular epidemiology of Staphylococcus aureus, MRSA having novel enterotoxins and virulence factors
Grant number：18K10054 2018.4 - 2021.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Aung Meiji Soe
Grant amount：\4420000 （ Direct Cost: \3400000 、 Indirect Cost：\1020000 ）
Prevalence of novel staphylococcal enterotoxin (SE)(-like) toxin genes in Staphylococcus aureus and its relative species S. argenteus, and their molecular epidemiological characteristics were analyzed. selw and selx were detected in more than 80% of S. aureus isolates examined, and showed diversity in their nucleotide sequences. S. aureus harbored sey and selz at low rate, without having sel26 and sel27. The sey gene was detected in all the ST2250 S. argenteus isolates, and more than half of this lineage harbored selz, sel26, and sel27. Prevalence of novel SE(-like) genes was revealed to be different depending on lineages of S. aureus and S. argenteus.
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How the staphylococcal species acquire resistance, and how they exchange genetic information?
Grant number：18K10055 2018.4 - 2021.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Urushibara Noriko
Grant amount：\4290000 （ Direct Cost: \3300000 、 Indirect Cost：\990000 ）
MRSA infection is an ongoing challenge. Staphylococcal species exchange genetic information through staphylococcal cassette chromosome (SCC). It encompasses not only drug resistance genes but also diverse genes useful for better adaptation to the unfriendly environment. The present study has aimed to reveal how the mobile genetic elements emerged, evolved, and spread among staphylococcal species. SCC elements obtained from MRSA clinical isolates were determined for their nucleotide sequences, and compared with staphylococcal genomes deposited in a public databank. Following two novel elements were identified: a novel subtype of type IX SCC carrying mec gene (SCCmec) in Myanmar MRSA, an SCC in MRSA from including speG and a staphylococcal surface protein coding gene (sasG) in Japanese MRSA. Obtained data suggests that they have originated in coagulase-negative staphylococcal species.
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Analysis of present status and comprehensive molecular epidemiology of emerging drug-resistant bacteria in developing regions of Asia and Caribbean province
Grant number：17H04664 2017.4 - 2020.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Kobayashi Nobumichi
Grant amount：\17940000 （ Direct Cost: \13800000 、 Indirect Cost：\4140000 ）
In three countries in Asia and Caribbean regions (Bangladesh, Myanmar, and Cuba) where information on antimicrobial resistance (AMR) is insufficient, resistance rates to various antimicrobials, prevalence of genes associated with AMR and virulence, genetic characteristics of drug-resistant isolates were analyzed for major pathogenic bacterial species (Escherichia coli, Klebsiella pneumoniae, Acinetobacter, Staphylococccus aureus, Enterococcus). As a result, it was revealed that various beta-lactamase genes have been widely distributed to Gram-negative rods represented by E. coli, and virulence factor PVL (Panton-Valentine leukocidin) associated with increased pathogenicity has been prevalent at relatively high rate among methicillin-resistant S. aureus (MRSA).
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Molecular epidemiological study of pneumococcal serotypes, drug resistance, and surface antigen for novel vaccine development
Grant number：16K09101 2016.4 - 2019.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Kawaguchiya Mitsuyo, ITO Masahiko
Grant amount：\4810000 （ Direct Cost: \3700000 、 Indirect Cost：\1110000 ）
After the introduction of the pneumococcal conjugate vaccine (PCV) for children, an increase in pneumococcal diseases caused by non-PCV serotypes is a worldwide concern. The prevalence of serotypes, antimicrobial resistance, and its relevance to PspA which is a considered as promising vaccine candidate were investigated for 729 (678 children, 51 adults) non-invasive pneumococcal isolates. Isolates were collected in hospitals and clinics in Hokkaido between June and November 2016. As a results, 87.9% of isolates from children belonged to non-PCV13 serotypes and most isolates (81-100%) of the dominant serotypes 15A, 35B and 23A were non-susceptible to penicillin. While most of the isolates (99%) had PspA belonging to either family 1 or 2, PspA family 3/clade 6 was detected in only non-PCV13 serotype 37 isolates assigned to ST447 and ST7970 showing the mucoid phenotype.
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Characterization of Novel Staphylococcal Chromosomal Cassette (SCC) Composite Islands in MRSA clinical isolates, and application to molecular epidemiology
Grant number：15K08781 2015.4 - 2019.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Urushibara Noriko
Grant amount：\4940000 （ Direct Cost: \3800000 、 Indirect Cost：\1140000 ）
The staphylococcal cassette chromosome (SCC) is a bacterial mobile genetic element inserted in the genome of staphylococcal species. SCC contains a variety of functional genes that provide biological benefits to the host bacteria. Staphylococcal species exchange genetic information by horizontal gene transfer of SCCs, which enables rapid adaption to changing environments. Three ST5-MRSA strains (SC640, SC792, and SC955) positive for both ACME-arc and speG were subjected to whole-genome sequencing, and their SCC composite islands (SCC-CIs) were determined. The SCC-CIs of these strains contained following novel SCCs: novel SCCs containing speG gene (SC640, SC792, and SC955), a novel SCCmec type XIV (SC792), and a truncated SCCmec type XIV-related structure (SC640).
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Study on prevalence and molecular epidemiological characteristics of novel community-acquired MRSA that emerged in Japan
Grant number：26460804 2014.4 - 2018.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Kobayashi Nobumichi, KAWAGUCHIYA Mitsuyo, AUNG Meijisoe, ITO Masahiko, SHINAGAWA Masaaki
Grant amount：\4940000 （ Direct Cost: \3800000 、 Indirect Cost：\1140000 ）
Methicillin-resistant Staphylococcus aureus (MRSA) is drug-resistant bacteria known for a long time. Recently, two MRSA clones with novel genetic traits (①MRSA harboring SCCmec-IVl which is a novel genetic complex; ②Coagulase type-II MRSA having ACME which is a genetic complex related to attachment to human skin) have been reported. We investigated their prevalence in clinical isolates in Hokkaido, Japan. These MRSA ①, ② were detected in 3.5% and 5.1% of isolates from outpatients of community, and 3% and 0.9% of isolates from a university hospital, respectively. Genetic diversity was found in MRSA with ACME, and their spread in the community and hospitals was concerned.
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Molecular epidemiology and population dynamics of rotaviruses in Asia based on novel comprehensive whole genomic analysis method
Grant number：25305022 2013.4 - 2017.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Nobumichi Kobayashi, AIDA Satoru, WANG Yuanhong, PAUL Shyamal
Grant amount：\17550000 （ Direct Cost: \13500000 、 Indirect Cost：\4050000 ）
Epidemic dynamics for long periods of human rotaviruses in Asia was analyzed molecular epidemiologically based on whole genome (11 gene segments). In Wuhan, China, G3P[8] was the most prevalent genotype of rotaviruses over past 10 years, which was thereafter replaced by G9P[8]. Change of lineages was detected for most of their gene segments with the passage of time. In Bangladesh, two lineages of the most prevalent G2P[4] genotype in 2010 were replaced by a novel lineage after 3 years. These findings indicated that replacement of gene segments via reassortment and mutation occurred over time among rotaviruses with prevalent genotypes. It was suggested that genetic changes have been occurring constantly even among the same genotype of rotaviruses.
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Characterization of novel ACME-SCCmec composite Islands in Japanese MRSA isolates: determination of genome structure and application to epidemiological studies.
Grant number：24590751 2012.4 - 2016.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Urushibara Noriko, KOBAYASHI Nobumichi
Grant amount：\5460000 （ Direct Cost: \4200000 、 Indirect Cost：\1260000 ）
The Arginine catabolic mobile element (ACME), a genomic island identified in the Staphylococci genome, is characteristically found in strain USA300, which is the predominant MRSA clone in the USA and is spreading globally. In the present study, the prevalence of ACME and the genetic diversity of ACME (+) MRSA were investigated for clinical isolates collected in Hokkaido.
The percentage of ACME carriage against the all MRSA isolates significantly increased in the course of study. Increased number of MRSA with the identical genotype as USA300 was observed, which suggests its potential spread in northern Japan. We focused on the ACME (+) MRSA with type II SCCmec (MRSA-II) because MRSA-II is the predominant genotype in Japanese MRSA. The genetic structures of ACME and its flanking region in MRSA-II were determined and novel genetic variants were identified. The similar analysis was carried out on coagulase-negative staphylococci clinical isolates obtained in Hokkaido. -
Identification and molecular epidemiological analysis of emerging clones of community-acquired MRSA
Grant number：23590746 2011 - 2013
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
KOBAYASHI Nobumichi, SUMI Ayako, URUSHIBARA Noriko, GHOSH Souvik
Grant amount：\5460000 （ Direct Cost: \4200000 、 Indirect Cost：\1260000 ）
To investigate prevalence in Japan of community-acquired MRSA (CA-MRSA) which is concerned for its global spread, and their molecular epidemiological characteristics, we analyzed clinical isolates from the Sapporo Medical University Hospital (601 isolates, 2008-2010) and those from local hospitals/clinics in Hokkaido prefecture (outpatients, 422 isolates in 2011). As a result, SCCmec types IV and V, which are genetic markers of CA-MRSA, were detected in 4.3% and 17.1% in the isolates from the University Hospital and local hospitals/clinics, respectively. Among them, seven isolates were revealed to be USA300 clone which is dominant in the US (ST8, positive for PVL and ACME), and many ST5 MRSA having ACME were detected. These emerging CA-MRSA clones were confirmed to be distributed in Japan.
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Infection dynamics and molecular mechanisms of prevalence of new variant and emerging Rotaviruses prevailing in Asia
Grant number：22406017 2010 - 2012
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
KOBAYASHI Nobumichi, ISHINO Masaho, SUMI Ayako, URUSHIBARA Noriko, GHOSH Souvik
Grant amount：\19500000 （ Direct Cost: \15000000 、 Indirect Cost：\4500000 ）
To elucidate genetic characteristics of new variants and emerging types of rotavirus that spread to mainly in Asian countries, whole genomic sequence analysis and phylogenetic analysis were conducted. In China and Bangladesh, the predominant genotypes were G1P[8]/G3P[8], G2P[4], respectively, among which reassortant viruses containing gene segments derived from animal rotavirus were detected. Rotaviruses with P[8]b, a new variant of P[8]-VP4 genotype, were detected in China, Bangladesh, and Myanmar, and mostly associated with globally emerging type G9. Whole genomic analysis of various rotaviruses strains with emerging genotypes from Asia and other regions (genotypes G1P[6], G2P[6], G3P[2]、G3P[6], G3P[9], G4P[10], G6P[9], G8P[1], G9P[19]) revealed that these strains could be classified into 1)reassortant between different human rotavirus genogroups, 2)reassortant between human and animal rotaviruses, and 3) animal rotaviruses that directly transmitted to humans. These findings indicated that reassortment and interspecies transmission may be the main causing mechanisms of new variants and emergingtypes of human rotaviruses.
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Study on prevalence of PVL-positive Staphylococcus aureus and MRSA with the use of novel molecular epidemiologic methods
Grant number：20590608 2008 - 2010
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
KOBAYASHI Nobumichi, ISHINO Masaho, SUMI Ayako, NAGASHIMA Mshigeo, URUSHIBARA Noriko
Grant amount：\4810000 （ Direct Cost: \3700000 、 Indirect Cost：\1110000 ）
Prevalence and molecular epidemiologic characteristics of methicillin-resistant S.aureus (MRSA) were analyzed for numerous Staphylococcus aureus isolates derived from outpatients in hospitals in Hokkaido. As a result, several MRSA strains (genotypes ST6, ST59) which harbor the Panton-Valentine Leukocidin (PVL) gene typical to CA-MRSA, and the genetic element ACME unique to USA300 CA-MRSA clone predominating in USA,were identified, suggesting the wide distribution of these MRSA to the community.
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Mechanisms of the regulation of small G-proteins in platelets
Grant number：19591092 2007 - 2008
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
ODA Atsushi, URUSHIBARA Noriko
Grant amount：\4550000 （ Direct Cost: \3500000 、 Indirect Cost：\1050000 ）
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ストレスの定量的評価の試み
Grant number：19659147 2007 - 2008
日本学術振興会 科学研究費助成事業 萌芽研究
藤田 博美, 小田 淳, 若尾 宏, 漆原 範子
Grant amount：\3300000 （ Direct Cost: \3300000 ）
成果の一部を上げる. まず, ニジマス由来RTG-2細胞の環境ストレスに対する応答を解析した. 化学汚染物質トリブチルスズ(TBT)がRTG-2にMAP kinase (ERK, JNK, p38 MAP kinase)の活性化を惹起すること, caspase活性化を伴うapoptosisを誘導することを明らかにした. さらにJNKがTBTによるcaspase依存的なapoptosisに, p38 MAP kinaseがcaspase非依存的な細胞死に関与することを明らかにした. 更に熱ショックに対する応答の解析を行った.
次に, 生物進化の過程でヒトを含めて広く保存されてきた様々な環境ストレスに対して共通する検知・応答機構について, 細胞骨格と細胞接着に注目して検討を進めた. 即ち, 良く保存されているArf-GAPタンパクGIT1とアダプタータンパクNckの結合様態と生理的意義について, 分子細胞生物学的な検討を行い, GIT1のチロシンリン酸化がNickを結合パートナーとして細胞内局在の移動をもたらし細胞機能を発揮していることが示唆された. これらのタンパクは線虫から哺乳動物まで重要な役割を果たしており, 普遍性の高いものであることが推察される. -
Elucidation of the physiological significance of novel platelet proteins.
Grant number：17590976 2005 - 2006
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
ODA Atsushi, NAKAYAMA Akira, URUSHIBARA Noriko
Grant amount：\3500000 （ Direct Cost: \3500000 ）
Because we have performed numerous experiments, we would like to focus on two following projects..In 2005, using specific antibodies against isoforms of WAVE, we demonstrated that human platelets express all 3 isoforms. With the use of an in vitro pull-down technique, the src homology 3 domain of insulin receptor substrate p53 (IRSp53) precipitated WAVE2 from platelet lysates more efficiently than did profilin I. The opposite was true for WAVE1, and neither precipitated WAVE3, suggesting that WAVE isoforms have different affinities to these ligands, while the SH3 domain of abl binds to all 3 isoforms. We also found that all 3 WAVE isoforms are substrates for calpain in vivo and in vitro. Although portions of these 3 isoforms were commonly distributed in the actin-and Arp2/3-rich edge of the lamellipodia in spreading platelets, only WAVE2 remained in the cell fringe following detergent extraction or fixation of the cells. These data suggest that the 3 WAVE isoforms exhibit common and distinct features and may potentially be involved in the regulation of actin cytoskeleton in platelets. In 2006, we also found that platelets express IRSp53 protein. We found that the knockdown of IRSp53 by RNA interference decreased lamellipodium formation without a decrease in the amount of WAVE2 complex. Localization of WAVE2 at the cell periphery was retained in IRSp53 knockdown cells. Moreover, activated Cdc42 but not Rac weakened the association between WAVE2 and IRSp53. When we measured Arp2/3 activation in vitro, the WAVE2 complex isolated from the membrane fraction of cells was fully active in an IRSp53-dependent manner but WAVE2 isolated from the cytosol was not. Purified WAVE2 and purified WAVE2 complex were activated by IRSp53 in a Rac-dependent manner with PIP(3)-containing liposomes. Therefore, IRSp53 optimizes the activity of the WAVE2 complex in the presence of activated Rac and PIP(3).