Updated on 2025/08/22

写真a

 
KURONUMA Kouji
 
Organization
School of Medicine Department of Respiratory Medicine and Allergology Associate Professor
Title
Associate Professor
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Research Interests

  • Toll様受容体

  • 肺線維症

  • 間質性肺炎

  • ブレオマイシン肺障害モデル

  • 非閉塞性肺疾患癌

  • 非感染性肺障害

  • 肺サーファクタント

  • 呼吸器感染症

  • 薬剤性肺障害

  • 肺コレクチン

  • トル様受容体

  • 肺サーファクタント蛋白質A

  • ブレオマイシン

Research Areas

  • Life Science / Respiratory medicine

Research History

  • Sapporo Medical University School of Medicine   Department of Respiratory Medicine and Allergology   Associate Professor

    2020

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  • Sapporo Medical University School of Medicine   Department of Respiratory Medicine and Allergology   Lecturer

    2015 - 2020

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  • Sapporo Medical University   School of Medicine   Assistant Professor

    2009 - 2015

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Papers

  • Rapid and Integrated Bacterial Evolution Analysis unveils gene mutations and clinical risk of Klebsiella pneumoniae. International journal

    Kojiro Uemura, Toyotaka Sato, Soh Yamamoto, Noriko Ogasawara, Jirachaya Toyting, Kotaro Aoki, Akira Takasawa, Masayuki Koyama, Atsushi Saito, Takayuki Wada, Kaho Okada, Yurie Yoshida, Koji Kuronuma, Chie Nakajima, Yasuhiko Suzuki, Motohiro Horiuchi, Kenichi Takano, Satoshi Takahashi, Hirofumi Chiba, Shin-Ichi Yokota

    Nature communications   16 ( 1 )   2917 - 2917   2025.3

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    Bacteria continually evolve. Previous studies have evaluated bacterial evolution in retrospect, but this approach is based on only speculation. Cohort studies are reliable but require a long duration. Additionally, identifying which genetic mutations that have emerged during bacterial evolution possess functions of interest to researchers is an exceptionally challenging task. Here, we establish a Rapid and Integrated Bacterial Evolution Analysis (RIBEA) based on serial passaging experiments using hypermutable strains, whole-genome and transposon-directed sequencing, and in vivo evaluations to monitor bacterial evolution in a cohort for one month. RIBEA reveals bacterial factors contributing to serum and antimicrobial resistance by identifying gene mutations that occurred during evolution in the major respiratory pathogen Klebsiella pneumoniae. RIBEA also enables the evaluation of the risk for the progression and the development of invasive ability from the lung to blood and antimicrobial resistance. Our results demonstrate that RIBEA enables the observation of bacterial evolution and the prediction and identification of clinically relevant high-risk bacterial strains, clarifying the associated pathogenicity and the development of antimicrobial resistance at genetic mutation level.

    DOI: 10.1038/s41467-025-58049-1

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  • JANISでは把握が困難な中小規模病院における緑膿菌の薬剤耐性の状況

    藤谷 好弘, 富樫 篤生, 齋藤 充史, 黒沼 幸治, 高橋 聡

    日本感染症学会総会・学術講演会・日本化学療法学会学術集会合同学会プログラム・抄録集   98回・72回   np255 - np255   2024.5

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  • 間質性肺疾患における気管支肺胞洗浄液中の肺胞マクロファージの代謝活性についての検討

    萬谷 峻史, 齋藤 充史, 佐藤 達也, 山口 美樹, 亀田 優美, 佐久間 裕司, 黒沼 幸治, 千葉 弘文

    日本呼吸器学会誌   13 ( 増刊 )   341 - 341   2024.3

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  • 結核の診断に時間を要した肺外結核の2例

    小林 拓海, 齋藤 充史, 小玉 賢太郎, 高橋 守, 黒沼 幸治, 千葉 弘文

    日本呼吸器学会誌   13 ( 増刊 )   395 - 395   2024.3

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  • 当院改修時におけるアスペルギルス対策の評価

    黒沼 幸治, 中江 舞美, 韮澤 慎也, 富樫 篤生, 藤谷 好弘, 齋藤 充史, 高橋 聡

    感染症学雑誌   98 ( 2 )   281 - 281   2024.3

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  • 当院改修時におけるアスペルギルス対策の評価

    黒沼 幸治, 中江 舞美, 韮澤 慎也, 富樫 篤生, 藤谷 好弘, 齋藤 充史, 高橋 聡

    感染症学雑誌   98 ( 2 )   281 - 281   2024.3

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  • The impact of respiratory reactance in oscillometry on survival in patients with idiopathic pulmonary fibrosis. International journal

    Tatsuru Ishikawa, Hirotaka Nishikiori, Yuki Mori, Keiko Fujino, Atsushi Saito, Mamoru Takahashi, Koji Kuronuma, Shiro Hinotsu, Hirofumi Chiba

    BMC pulmonary medicine   24 ( 1 )   10 - 10   2024.1

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    BACKGROUND: Idiopathic pulmonary fibrosis (IPF) is a progressive disease with a poor prognosis. Pulmonary function tests (PFTs) aid in evaluating the disease status of IPF. The clinical significance of oscillometry measurements in interstitial lung diseases has recently been reported. Our previous study showed that respiratory reactance (Xrs) measured by oscillometry reflected disease severity and predicted subsequent lung capacity decline in patients with IPF. However, the direct impact of Xrs on survival needs to be determined, and there are currently no reference values in oscillometry to predict prognosis. Therefore, this study aimed to investigate the association between oscillometry measurements, particularly Xrs, and survival in patients with IPF and to determine the cutoff values of Xrs that predict 3-year survival. METHODS: We analyzed the relationship between the measured values of PFT and oscillometry derived from 178 patients with IPF. Univariate and multivariate Cox proportional hazards analyses were performed to investigate the relationships between clinical indices at the time of the first oscillometry and survival. We performed the time-dependent receiver operating characteristic (ROC) curve analysis to set the optimized cutoff values of Xrs for 3-year survival prediction. We examined the discriminating power of cutoff values of Xrs on survival using the Kaplan-Meier method and the log-rank test. RESULTS: Xrs components, especially in the inspiratory phase (In), significantly correlated with the PFT values. In the multivariate analyses, Xrs (all of reactance at 5 Hz [X5], resonant frequency [Fres], and low-frequency reactance area [ALX] in the inspiratory phase) had a significant impact on survival (X5, p = 0.003; Fres, p = 0.016; ALX, p = 0.003) independent of age, sex, and other prognostic factors derived from the univariate analysis. The area under the ROC curve was 0.765, 0.759, and 0.766 for X5 In, Fres In, and ALX In, with cutoff values determined at - 0.98, 10.67, and 5.32, respectively. We found significant differences in survival after dividing patients using each of the cutoff values of Xrs. CONCLUSIONS: In patients with IPF, Xrs measured by oscillometry significantly impacted survival. We also determined the cutoff values of Xrs to discriminate patients with poor prognoses.

    DOI: 10.1186/s12890-023-02776-y

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  • 手洗器の水栓口や給水システムの汚染による緑膿菌の感染伝播

    藤谷 好弘, 中江 舞美, 佐藤 勇樹, 韮澤 慎也, 富樫 篤生, 齋藤 充史, 黒沼 幸治, 高橋 聡

    感染症学雑誌   98 ( 1 )   119 - 119   2024.1

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  • Factors affecting the sensitivity of quantitative severe acute respiratory syndrome coronavirus 2 antigen test. International journal

    Yuki Sato, Ryosei Murai, Ryo Kobayashi, Atsuo Togashi, Yoshihiro Fujiya, Koji Kuronuma, Satoshi Takahashi

    Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy   29 ( 8 )   754 - 758   2023.8

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    INTRODUCTION: The accuracy of nucleic acid amplification tests (NAATs) is affected by various factors; however, studies examining the factors affecting the accuracy of quantitative severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigen test (QAT) are limited. METHODS: A total of 347 nasopharyngeal samples were collected from patients with coronavirus disease 2019 (COVID-19), and the date of onset was obtained from the electronic medical records. The SARS-CoV-2 antigen level was measured using Lumipulse Presto SARS-CoV-2 Ag (Presto), while NAAT was performed using the Ampdirect 2019-nCoV Detection Kit. RESULTS: Presto had a sensitivity rate of 95.1% (95% confidence interval: 92.8-97.4) in detecting the SARS-CoV-2 antigen in 347 samples. The number of days from symptom onset to sample collection was negatively correlated with the amount of antigen (r = -0.515) and sensitivity of Presto (r = -0.711). The patients' age was lower in the Presto-negative samples (median age, 39 years) compared with that in the Presto-positive samples (median age, 53 years; p < 0.01). A significant positive correlation was observed between age (excluding teenagers) and Presto sensitivity (r = 0.764). Meanwhile, no association was found between the mutant strain, sex, and Presto results. CONCLUSION: Presto is useful for the accurate diagnosis of COVID-19 owing to its high sensitivity when the number of days from symptom onset to sample collection is within 12 days. Furthermore, age may affect the results of Presto, and this tool has a relatively low sensitivity in younger patients.

    DOI: 10.1016/j.jiac.2023.04.005

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  • COVID-19患者における血清レクチン値についての検討

    竹中 遥, 齋藤 充史, 黒沼 幸治, 千葉 弘文

    日本肺サーファクタント・界面医学会雑誌   54   28 - 29   2023.8

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  • COVID-19患者における血清レクチン値についての検討

    竹中 遥, 齋藤 充史, 黒沼 幸治, 千葉 弘文

    日本肺サーファクタント・界面医学会雑誌   54   28 - 29   2023.8

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  • ゲノム解析は同時期に複数病棟で発生したCOVID-19クラスター事例の全体像の把握に有用である

    藤谷 好弘, 中江 舞美, 中村 広士, 富樫 篤生, 齋藤 充史, 黒沼 幸治, 高橋 聡, 大久保 和洋, 川代 愛梨, 田宮 和真

    日本環境感染学会総会プログラム・抄録集   38回   271 - 271   2023.6

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  • COVID-19の濃厚接触者が陽性となる要因の検討

    中江 舞美, 藤谷 好弘, 富樫 篤生, 中村 広士, 斎藤 充史, 黒沼 幸治, 高橋 聡

    日本環境感染学会総会プログラム・抄録集   38回   382 - 382   2023.6

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  • Pseudo-outbreak of Mycobacterium lentiflavum at a general hospital in Japan. International journal

    Yutaro Nagano, Koji Kuronuma, Yasuo Kitamura, Kanami Nagano, Hayato Yabe, Sayaka Kudo, Toyotaka Sato, Shinya Nirasawa, Mami Nakae, Motohiro Horiuchi, Shin-Ichi Yokota, Yoshihiro Fujiya, Atsushi Saito, Satoshi Takahashi, Hirofumi Chiba

    Infection control and hospital epidemiology   1 - 7   2023.4

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    BACKGROUND: Mycobacterium lentiflavum is a slow-growing nontuberculous mycobacterium that is widely distributed in soil and water systems, but it is sometimes pathogenic to humans. Although cases of M. lentiflavum infections are rare, 22 isolates of M. lentiflavum were identified at a single hospital in Japan. We suspected a nosocomial outbreak; thus, we conducted transmission pattern and genotype analyses. METHODS: Cases of M. lentiflavum isolated at Kushiro City General Hospital in Japan between May 2020 and April 2021 were analyzed. The patient samples and environmental culture specimens underwent whole-genome sequencing (WGS). Additionally, we retrospectively collected clinical data from patient medical records. RESULTS: Altogether, 22 isolates of M. lentiflavum were identified from sputum and bronchoalveolar lavage samples. Clinically, the instances with M. lentiflavum isolates were considered contaminants. In the WGS analysis, 19 specimens, including 18 patient samples and 1 environmental culture from the hospital's faucet, showed genetic similarity. The frequency of M. lentiflavum isolation decreased after we prohibited the use of taps where M. lentiflavum was isolated. CONCLUSIONS: WGS analysis identified that the cause of M. lentiflavum pseudo-outbreak was the water used for patient examinations, including bronchoscopy.

    DOI: 10.1017/ice.2023.68

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  • Klebsiella pneumoniaeにおける遺伝子変異頻度と病原性の関連性評価

    上村 幸二朗, 佐藤 豊孝, 黒沼 幸治, 高橋 聡, 横田 伸一

    日本化学療法学会雑誌   71 ( 2 )   179 - 179   2023.3

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  • SARS-CoV-2ワクチン接種後の長期的抗体価推移と次期接種のタイミングの検討

    藤谷 好弘, 中江 舞美, 富樫 篤生, 齋藤 充史, 黒沼 幸治, 高橋 聡

    日本感染症学会総会・学術講演会・日本化学療法学会学術集会合同学会プログラム・抄録集   97回・71回   O - 127   2023.3

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  • 間質性肺疾患を有する難治性咳嗽に対してのP2X3受容体拮抗薬の有効性の検討

    高橋 知之, 齋藤 充史, 萬谷 峻史, 池田 貴美之, 黒沼 幸治, 千葉 弘文

    日本呼吸器学会誌   12 ( 増刊 )   216 - 216   2023.3

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  • Analysis of diagnostic performance and factors causing nonspecific reactions in SARS-CoV-2 rapid antigen detection tests. International journal

    Natsuki Narumi, Takashi Kondo, Yuki Sato, Yuki Katayama, Shinya Nirasawa, Masachika Saeki, Yuki Yakuwa, Yoshihiro Fujiya, Koji Kuronuma, Satoshi Takahashi

    Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy   29 ( 2 )   157 - 162   2023.2

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    INTRODUCTION: Early diagnosis and appropriate infection control are important to prevent the transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In this study, we aimed to assess the diagnostic performance of SARS-CoV-2 rapid antigen detection (RAD) tests and the factors that cause nonspecific reactions. METHODS: Nasopharyngeal swab specimens (n = 100), sputum specimens (n = 10), and lithium-heparin plasma samples (n = 100) were collected. We evaluated Espline®SARS-CoV-2 (Espline) and SARS-CoV-2 Rapid Antigen Test that also known as STANDARD Q® (STANDARD Q), with reverse transcription-polymerase chain reaction (RT-PCR) and Lumipulse® Presto SARS-CoV-2 Ag as reference tests. In addition, we investigated the effects of inadequate pretreatment methods and five potential causes of nonspecific reactions. RESULTS: The sensitivities of Espline and STANDARD Q were 60% and 57%, respectively, and their specificity was 100%. It was confirmed that the judgment line for the positive insufficiently mixed specimens was faint. A false-positive result was observed with STANDARD Q when sputum was used as a specimen to investigate judgment the effect of viscosity. CONCLUSIONS: Espline and STANDARD Q show good sensitivity for specimens with Ct values less than 25, but specimens collected within 9 days of symptom onset may still give false negatives. The test should be performed carefully, and the results should be judged comprehensively, taking into account clinical symptoms and patient background.

    DOI: 10.1016/j.jiac.2022.10.007

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  • Protective effect of Bifidobacterium longum BB536 against nausea caused by pirfenidone in a mouse model of pellagra.

    Koji Kuronuma, Natsumi Susai, Tomohiro Kuroita, Takeshi Yoshioka, Atsushi Saito, Hirofumi Chiba

    Bioscience of microbiota, food and health   42 ( 3 )   195 - 202   2023

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    Pellagra is caused by abnormal intake and/or use of nicotinic acid and is known in part to be induced by the use of medications such as isoniazid or pirfenidone. We previously investigated atypical phenotypes of pellagra, such as nausea, using a mouse model of pellagra and found that gut microbiota play an important role in the development of these phenotypes. Here, we investigated the effect of Bifidobacterium longum BB536 on pellagra-related nausea caused by pirfenidone in our mouse model. Our pharmacological data indicated that pirfenidone (PFD) causes modulation of the gut microbiota profile, which appeared to play an important role in the development of pellagra-related nausea. A gut microbiota-mediated protective effect of B. longum BB536 against nausea caused by PFD was also identified. Finally, the urinary ratio of nicotinamide/N-methylnicotinamide was shown to be a biomarker of pellagra-like adverse effects induced by PFD, and it may contribute to the prevention of these effects in patients with idiopathic pulmonary fibrosis.

    DOI: 10.12938/bmfh.2022-042

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  • Serum Testosterone Is Associated With the Severity of COVID-19. International journal

    Ema Suzuki, Koji Kuronuma, Ryosei Murai, Yoshihiro Fujiya, Atsushi Saito, Hirofumi Chiba, Satoshi Takahashi

    In vivo (Athens, Greece)   37 ( 5 )   2314 - 2319   2023

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    BACKGROUND/AIM: Coronavirus disease 2019 (COVID-19) is more likely to be severe in men than in women. Its association with sex hormones as an aggravating factor for male patients has been attracting attention. This study aimed to investigate whether serum testosterone is associated with the aggravation of COVID-19. PATIENTS AND METHODS: Serum testosterone concentrations in 116 male patients with COVID-19 and residual serum were measured and examined upon their admission to Sapporo Medical University Hospital between February 1, 2020 and March 31, 2021. RESULTS: Blood samples collected from these patients with COVID-19 were analyzed. The serum testosterone levels were 2.19±1.35, 1.29±0.88, and 0.75±0.58 ng/ml in mild, moderate, and severe groups, respectively. Patients with severe COVID-19 on admission had lower testosterone levels (p<0.001). At a cutoff level of 1.31 ng/ml, the area under the curve for the comparison of severe with non-severe cases was 0.825. Furthermore, serum testosterone levels negatively correlated with C-reactive protein and serum amyloid A levels but positively correlated with calcium, zinc, C3, and C4. CONCLUSION: In male patients with COVID-19, low serum testosterone levels correlated with disease severity, accompanied by a strong inflammatory reaction and proportion of complement consumption.

    DOI: 10.21873/invivo.13334

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  • The Soluble Lectin Families as Novel Biomarkers for COVID-19 Pneumonia. International journal

    Haruka Takenaka, Atsushi Saito, Koji Kuronuma, Keigo Moniwa, Hirotaka Nishikiori, Satoshi Takahashi, Hirofumi Chiba

    In vivo (Athens, Greece)   37 ( 4 )   1721 - 1728   2023

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    BACKGROUND/AIM: The lung-specific soluble lectins, SP-A and SP-D have been clinically used to diagnose interstitial lung disease, but their clinical significance in COVID-19 remains controversial. This study was undertaken to determine their association with other lectins (MBL and FCN1), disease severity, and radiographs in COVID-19 patients. PATIENTS AND METHODS: A total of 131 patients with COVID-19 admitted in the Sapporo Medical University Hospital between May 22 and September 19, 2021, were enrolled in the study. Data including demographics, medical history, symptoms, signs, laboratory findings, and radiological images were collected from the patients' medical records. Chest computed tomography (CT) scanning was performed at admission. Serum levels of surfactant protein A and D (SP-A and SP-D), mannose-binding lectin (MBL) and ficolin1 (FCN1) were measured using enzyme-linked immunosorbent assay (ELISA) kits. RESULTS: Compared to the control group, the COVID-19 group had significantly higher serum SP-A and FCN1 levels on admission (SP-A: 59.60±38.89 vs. 35.61±11.22 ng/ml; p<0.01, FCN1: 542.45±506.04 vs. 250.6±161.1 ng/ml; p<0.01). The severe group in COVID-19 had significantly higher serum SP-D and lower MBL levels than the non-severe group (SP-D: 141.7±155.7 vs. 61.41±54.54 ng/ml; p<0.01, MBL: 1,670±1,240 vs. 2,170±1,140 ng/ml; p<0.05). SP-D strongly reflected the degree of imaging findings, whereas SP-A showed a significant correlation, albeit slightly weaker than SP-D. Conversely, MBL and FNC1 were not significantly correlated with imaging findings. CONCLUSION: Among soluble serum lectins, SP-A and SP-D may be more sensitive to CT findings than reported disease biomarkers such as IL-6, LDH, and CRP due to their lung-specific characteristics.

    DOI: 10.21873/invivo.13259

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  • 男性COVID-19患者の血清テストステロン値は重症度と関連する

    黒沼 幸治, 鈴木 瑛真, 村井 良精, 中江 舞美, 藤谷 好弘, 齋藤 充史, 高橋 聡

    日本感染症学会東日本地方会学術集会・日本化学療法学会東日本支部総会合同学会プログラム・抄録集   71回・69回   125 - 125   2022.10

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  • 当院における10年間のvanC保有腸球菌菌血症の特徴から見る治療において重要なこと

    藤谷 好弘, 佐藤 勇樹, 富樫 篤生, 齋藤 充史, 黒沼 幸治, 高橋 聡

    日本感染症学会東日本地方会学術集会・日本化学療法学会東日本支部総会合同学会プログラム・抄録集   71回・69回   118 - 118   2022.10

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  • COVID-19における血清レクチン値の臨床バイオマーカーとしての有用性についての検討

    齋藤 充史, 黒沼 幸治, 富樫 篤生, 藤谷 好弘, 高橋 聡

    日本感染症学会東日本地方会学術集会・日本化学療法学会東日本支部総会合同学会プログラム・抄録集   71回・69回   125 - 125   2022.10

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  • Pneumocystis jirovecii Pneumonia Associated with COVID-19 in Patients with Interstitial Pneumonia. International journal

    Tomoyuki Takahashi, Atsushi Saito, Koji Kuronuma, Hirotaka Nishikiori, Hirofumi Chiba

    Medicina (Kaunas, Lithuania)   58 ( 9 )   2022.8

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    Here, we report two cases of patients with interstitial pneumonia (IP) on steroids who developed Pneumocystis jirovecii pneumonia (PJP) following coronavirus disease 2019 (COVID-19) infection. Case 1: A 69-year-old man on 10 mg of prednisolone (PSL) daily for IP developed new pneumonia shortly after his COVID-19 infection improved and was diagnosed with PJP based on chest computed tomography (CT) findings and elevated serum β-D-glucan levels. Trimethoprim-sulfamethoxazole (TMP-SMZ) was administered, and the pneumonia resolved. Case 2: A 70-year-old woman taking 4 mg/day of PSL for IP and rheumatoid arthritis developed COVID-19 pneumonia, which resolved mildly, but her pneumonia flared up and was diagnosed as PJP based on CT findings, elevated β-D-glucan levels, and positive polymerase chain reaction for P. jirovecii DNA in the sputum. The autopsy revealed diffuse alveolar damage, increased collagen fiver and fibrotic foci, mucinous component accumulation, and the presence of a P. jirovecii cyst. In conclusion, steroids and immunosuppressive medications are well-known risk factors for PJP. Patients with IP who have been taking these drugs for a long time are frequently treated with additional steroids for COVID-19; thus, PJP complications should be avoided in such cases.

    DOI: 10.3390/medicina58091151

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  • Genotypes and transmission routes of noroviruses causing sporadic acute gastroenteritis among adults and children, Japan, 2015-2019. International journal

    Saho Honjo, Koji Kuronuma, Yoshihiro Fujiya, Mami Nakae, Susumu Ukae, Hiroshi Nihira, Masaki Yamamoto, Yusuke Akane, Kenji Kondo, Satoshi Takahashi, Hirokazu Kimura, Hiroyuki Tsutsumi, Yukihiko Kawasaki, Takeshi Tsugawa

    Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases   104   105348 - 105348   2022.8

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    Noroviruses (NoVs) are major causes of acute viral gastroenteritis at all ages worldwide. The molecular epidemiology of sporadic cases remains poorly understood, especially in adults. Additionally, no studies have analyzed the transmission route in sporadic acute gastroenteritis. In this study, we investigated cases of very mild sporadic NoV acute gastroenteritis in adults (medical staff) who do not visit the outpatient clinic and child outpatients. We also evaluated genotype differences between adults and children and possible transmission routes in adults during 5 years. The number of NoV positives were 58 in adults and 124 in children. In adults, the NoV positivity rate in this study was higher (64.4%) than that in previous reports of outpatients (10%) and inpatients (5%) in the United State. This finding suggested that the NoV positivity rate might be high in adults with very mild acute gastroenteritis. In adults, human-to-human transmission rates from children and food-borne transmission (raw oysters) were 21.6% (11/51) and 19.6% (10/51), respectively. Among adults, GII.2, GII.4, and GII.17 were the predominant genotypes, with rates of 32.7%, 30.9%, and 21.8%, respectively. Among children, GII.4 and GII.2 were the predominant genotypes, with rates of 45.5% and 40.6%, respectively. GII.17 was only detected in 0.8% (1/123) of children. Trends in NoV genotypes are expected to differ depending on the patient's age. Investigating sporadic cases including the patient's background (age and transmission route) may be helpful to monitor the trend of NoV strains, forecast prevalent NoV GII genotypes, and develop NoV vaccines.

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  • Viral load may impact the diagnostic performance of nasal swabs in nucleic acid amplification test and quantitative antigen test for SARS-CoV-2 detection. International journal

    Yoshihiro Fujiya, Yuki Sato, Yuki Katayama, Shinya Nirasawa, Mikako Moriai, Masachika Saeki, Yuki Yakuwa, Ikumi Kitayama, Koichi Asanuma, Koji Kuronuma, Satoshi Takahashi

    Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy   28 ( 11 )   1590 - 1593   2022.8

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    INTRODUCTION: Compared to nasopharyngeal swabs (NPS), there has been insufficient evaluation of the diagnostic performance of nasal swabs (NS) for the detection of severe acute respiratory coronavirus 2 (SARS-CoV-2) in the nucleic acid amplification test (NAAT) and quantitative SARS-CoV-2 antigen test (QAT). METHODS: We prospectively compared healthcare worker-collected and flocked NS within nine days after symptom onset to paired NPS to detect SARS-CoV-2 in NAAT and QAT on the fully automated Lumipulse system. The agreement between sample types was evaluated, and cycle threshold (Ct) values and antigen levels were used as surrogate viral load measures. RESULTS: Sixty sets of NPS and NS samples were collected from 40 patients with COVID-19. The overall agreements between NAAT and QAT samples were 76.7% and 65.0%, respectively. In NAAT, the Ct value of NS was significantly higher, 5.9, than that of NPS. Thirty-nine (95.1%) NS tested positive in 41 positive-paired NPS with Ct ≤ 30. The negative correlation was observed between antigen levels of NS in QAT and Ct values of NS in NAAT (r = -0.88). In QAT, the antigen level of NS was significantly lower than that of NPS. Thirty-six (90.0%) NS tested positive in 40 positive-paired NPS with antigen levels >100 pg/mL, which were collected significantly earlier than those with antigen levels ≤100 pg/mL. CONCLUSIONS: In NAAT and QAT, NS had limited performance in detecting SARS-CoV-2 compared to NPS. However, NS may be helpful for patients with COVID-19 with high viral loads or those in the early stages of the illness.

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  • Comparative study of rapid antigen testing and two nucleic acid amplification tests for influenza virus detection. International journal

    Yuki Sato, Shinya Nirasawa, Masachika Saeki, Yuki Yakuwa, Mayumi Ono, Ryo Kobayashi, Hirotaka Nakafuri, Ryosei Murai, Yoshihiro Fujiya, Koji Kuronuma, Satoshi Takahashi

    Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy   28 ( 7 )   1033 - 1036   2022.7

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    INTRODUCTION: This study aimed to evaluate rapid antigen detection (RAD) and rapid nucleic acid amplification tests (NAATs) to detect influenza virus (IV). METHODS: The conventional RAD test (Quick Chaser Flu A, B: QC), using silver amplified immunochromatography (Quick Chaser Auto Flu A, B: QCA), as well as two NAATs (Xpert Xpress Flu/RSV: Xpert, cobas Influenza A/B & RSV: cobas) were evaluated using nasopharyngeal swabs from suspected cases of influenza. A reference method was performed using real-time reverse transcription polymerase chain reaction according to the manual of the Japanese National Institute of Infectious Disease (NIID). RESULTS: From a total of 177 samples, 51 were positive according to the NIID assay. The kappa (κ) coefficient in Xpert and cobas for influenza A virus (IAV)/influenza B virus (IBV) was 1.00, which was the highest among the four detection assays. However, the κ coefficients in QC and QCA for IAV/IBV were 0.71-0.77 and 0.87-0.89, respectively. The sensitivities of the RAD tests were 41.7% in QC and 50.0% in QCA at < 6 h after onset, and 100.0% in both QC and QCA at 24-48 h after onset. The cycle threshold (Ct) values were significantly lower in the group in which all detection assays were positive for IAV. CONCLUSIONS: Xpert and cobas have comparable analytical performances and are highly useful as influenza virus detection assays. QC and QCA could show false negatives frequently in the early stage of infection and when viral load is low.

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  • Does the timing of saliva collection affect the diagnosis of SARS-CoV-2 infection? International journal

    Yuki Katayama, Ryosei Murai, Mikako Moriai, Shinya Nirasawa, Masachika Saeki, Yuki Yakuwa, Yuki Sato, Koichi Asanuma, Yoshihiro Fujiya, Koji Kuronuma, Satoshi Takahashi

    Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy   28 ( 7 )   1012 - 1014   2022.7

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    We evaluated the optimal timing of saliva sample collection to diagnose the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. We obtained 150 saliva samples at four specific time points from 13 patients with confirmed SARS-CoV-2 infection. The time points were (1) early morning (immediately after waking), (2) immediately after breakfast before tooth brushing, (3) 2 h after breakfast, and (4) before lunch. On the 2nd hospital day, patients collected saliva at the four time points by themselves. We collected samples at two time points, (1) and (3), from the 3rd hospital day to day 9 following symptom onset. In 52 samples collected at the four time points, there was no significant difference. Meanwhile, there was no significant difference in the positive proportion or the viral load between the two time points in both analyses by the day from symptom onset and by all samples. In this study, there was no difference in the positive proportions in saliva collected at various time points within 9 days after symptom onset. The timing of saliva collection was not affected by the diagnosis of SARS-CoV-2 infection.

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  • COVID-19肺炎重症度予測マーカーとしてのIL-6、INF-λ3、NGALの検討

    黒沼 幸治, 中江 舞美, 藤谷 好弘, 高橋 聡, 齋藤 充史, 安田 健人, 千葉 弘文

    日本化学療法学会雑誌   70 ( Suppl.A )   299 - 299   2022.5

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  • COVID-19 臨床経過予測因子 COVID-19肺炎重症度予測マーカーとしてのIL-6、INF-λ3、NGALの検討

    齋藤 充史, 安田 健人, 黒沼 幸治, 中江 舞美, 藤谷 好弘, 高橋 聡, 千葉 弘文

    日本呼吸器学会誌   11 ( 増刊 )   153 - 153   2022.4

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  • COVID-19 臨床経過予測因子 COVID-19肺炎重症度予測マーカーとしてのIL-6、INF-λ3、NGALの検討

    齋藤 充史, 安田 健人, 黒沼 幸治, 中江 舞美, 藤谷 好弘, 高橋 聡, 千葉 弘文

    日本呼吸器学会誌   11 ( 増刊 )   153 - 153   2022.4

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  • 胸部X線写真から線維化性間質性肺疾患を検出するAIソフトウェアの性能検証研究

    錦織 博貴, 黒沼 幸治, 廣田 健一, 鈴木 朋浩, 池田 貴美之, 森 勇樹, 浅井 悠一郎, 本田 聖和, 千葉 弘文

    日本呼吸器学会誌   11 ( 増刊 )   301 - 301   2022.4

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  • N-glycosylation regulates MET processing and signaling. International journal

    Atsushi Saitou, Yoshihiro Hasegawa, Naoki Fujitani, Shigeru Ariki, Yasuaki Uehara, Ukichiro Hashimoto, Atsushi Saito, Koji Kuronuma, Kunio Matsumoto, Hirofumi Chiba, Motoko Takahashi

    Cancer science   113 ( 4 )   1292 - 1304   2022.1

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    MET, the receptor for the hepatocyte growth factor (HGF), is strongly associated with resistance to tyrosine kinase inhibitors, key drugs that are used in the therapy of non-small cell lung cancer. MET contains 11 potential N-glycosylation sites, but the site-specific roles of these N-glycans have not been elucidated. We report herein that these N-glycans regulate the proteolytic processing of MET and HGF induced MET signaling, and that this regulation is site specific. Inhibitors of N-glycosylation were found to suppress the processing and trafficking of endogenous MET in H1975 and EBC-1 lung cancer cells and exogenous MET in CHO-K1 cells. We purified the recombinant extracellular domain of human MET and determined the site-specific N-glycan structures and occupancy using mass spectrometry. The results indicated that most sites were fully glycosylated and that the dominant population was the complex-type. To examine the effects of the deletion of N-glycans of MET, we prepared endogenous-MET-knockout Flp-In CHO cells and transfected them with a series of N-glycan deletion mutants of MET. The results showed that several N-glycans are implicated in the processing of MET. The findings also suggested that the N-glycans of the SEMA domain of MET positively regulate HGF signaling, and the N-glycans of the region other than the SEMA domain negatively regulate HGF signaling. Processing, cell surface expression, and signaling were significantly suppressed in the case of the all-N-glycan deletion mutant. The overall findings suggest that N-glycans of MET affect the status and the function of the receptor in a site-specific manner.

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  • ファビピラビルおよびデキサメタゾン投与開始時期とCOVID-19臨床転帰の関連性

    伊部 裕太, 石郷 友之, 藤居 賢, 藤谷 好弘, 黒沼 幸治, 高橋 聡

    日本化学療法学会雑誌   70 ( 1 )   164 - 165   2022.1

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  • Self-reported Smell and Taste Disorders in Patients With COVID-19: A Japanese Single-center Study. International journal

    Keisuke Yamamoto, Yoshihiro Fujiya, Koji Kuronuma, Noriko Ogasawara, Tsuyoshi Ohkuni, Shin-Ichi Yokota, Satoshi Takahashi, Kenichi Takano

    In vivo (Athens, Greece)   36 ( 2 )   918 - 924   2022

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    BACKGROUND/AIM: Smell and taste disorders are among the most common symptoms of COVID-19. However, the relationship between smell and taste disorders and systemic symptoms is not fully understood in Japan. PATIENTS AND METHODS: Questionnaires were mailed to 105 of 111 COVID-19 patients who were hospitalized at our hospital between March and July 2020 in Japan. RESULTS: A total of 74 patients (response rate: 70.5%) completed the survey. Of these, six patients (8.1%) presented with smell disorders only, 16 (21.6%) presented with taste disorders only, and 17 (23.0%) presented with both smell and taste disorders. The mean Visual Analog Scale for smell and taste was 0.5 and 20, respectively, at the time of the most severe symptoms. CONCLUSION: Among COVID-19 patients in Japan, smell and taste disorders are often followed by fever and may not be the first symptoms. Sense of smell is particularly impaired. These symptoms often improve, although they sometimes persist for a long time as sequelae.

    DOI: 10.21873/invivo.12781

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  • 【肺免疫から肺疾患を考える】肺疾患とマイクロバイオーム

    齋藤 充史, 黒沼 幸治, 千葉 弘文

    呼吸器内科   40 ( 6 )   551 - 556   2021.12

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  • 新型コロナウイルスN501Y変異株感染入院患者が従来の退院基準を満たした日におけるCt値および抗原量に関する検討

    藤谷 好弘, 佐藤 勇樹, 韮澤 慎也, 中村 広士, 中江 舞美, 津川 毅, 黒沼 幸治, 高橋 聡, 片山 雄貴, 米澤 仁, 村井 良精, 盛合 美加子, 四十坊 典晴

    病原微生物検出情報月報   42 ( 10 )   233 - 234   2021.10

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  • COVID-19肺炎におけるバイオマーカーとしての血清SP-AおよびSP-Dの有用性

    千葉 弘文, 齋藤 充史, 黒沼 幸治, 茂庭 慶悟, 高橋 弘毅

    日本肺サーファクタント・界面医学会雑誌   52   31 - 31   2021.10

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  • Early favipiravir treatment was associated with early defervescence in non-severe COVID-19 patients. International journal

    Satoshi Fujii, Yuta Ibe, Tomoyuki Ishigo, Hirotoshi Inamura, Yusuke Kunimoto, Yoshihiro Fujiya, Koji Kuronuma, Hiromasa Nakata, Masahide Fukudo, Satoshi Takahashi

    Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy   27 ( 7 )   1051 - 1057   2021.4

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    INTRODUCTION: The antiviral drug favipiravir has been shown to have in vitro antiviral activity against severe-acute-respiratory-syndrome-coronavirus-2 (SARS-CoV-2). In this study, we investigated the clinical benefits and initiation of favipiravir treatment in patients with non-severe coronavirus-disease-2019 (COVID-19). METHODS: This study was a single-center retrospective cohort study. Receiver operating characteristic curves were drawn to calculate the area under the curve, and the optimal cut-off values for the time to initiate favipiravir treatment were calculated to predict defervescence within seven days. Univariate and multivariate Cox regression analyses were performed to identify potential influencing factors of defervescence. This was defined as a body temperature of less than 37 °C for at least 2 days. RESULTS: Data from 41 patients were used for the efficacy assessment. The days from the onset of fever to defervescence showed a positive correlation with the duration from the onset of fever to initiation of favipiravir treatment (r = 0.548, P < 0.001). The optimal cut-off value was the administration of favipiravir on day 4. Patients were assigned to two groups based on the optimal cut-off value from onset to initiation of favipiravir treatment: early treatment group (within 4-days) and late treatment group (more than 4-days). In the multivariate analysis, when adjusted for age, sex, and days from onset to initiation of favipiravir treatment, the significant factors were male sex and days of initiation of the favipiravir treatment. CONCLUSIONS: We recommend that if favipiravir is to be used for treatment, it should be initiated as early as possible.

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  • Invasive pneumococcal disease affected the fatal outcome in a COVID-19 patient. International journal

    Koji Kuronuma, Naofumi Bunya, Bin Chang, Yoshihiro Fujiya, Kazunori Oishi, Eichi Narimatsu, Satoshi Takahashi, Hirofumi Chiba

    Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy   27 ( 7 )   1108 - 1111   2021.4

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    A 68-year-old man experienced fever and cough and was referred to a hospital for day 4. He had a positive reverse transcription-polymerase chain reaction result for severe acute respiratory syndrome coronavirus-2. On day 12, his PaO2/FiO2 ratio worsened to 120 and he was transferred to Sapporo Medical University Hospital for treatment using extracorporeal membrane oxygenation. Venous blood cultures were positive for Streptococcus pneumoniae, which were serotype 3, mucoid-type, and penicillin susceptible. Coinfections with coronavirus disease-2019 and invasive pneumococcal disease are rare; however, they are associated with a higher case fatality than either of the conditions manifesting alone.

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  • 【呼吸器疾患とマイクロバイオーム】特発性肺線維症における肺マイクロバイオーム研究の展開

    黒沼 幸治, 齋藤 充史, 千葉 弘文

    呼吸臨床   5 ( 4 )   1/5 - 5/5   2021.4

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  • COVID-19肺炎重症度予測マーカーとしての血清SP-A,D値の検討

    齋藤 充史, 黒沼 幸治, 小玉 賢太郎, 茂庭 慶悟, 高橋 聡, 高橋 弘毅, 千葉 弘文

    日本呼吸器学会誌   10 ( 増刊 )   272 - 272   2021.4

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  • ピルフェニドンの抗線維化作用におけるTRPV4の役割

    黒沼 幸治, 大塚 満雄, 若林 雅人, 吉岡 健, 小林 智史, 亀田 優美, 森岡 靖英, 千葉 弘文, 高橋 弘毅

    日本呼吸器学会誌   10 ( 増刊 )   258 - 258   2021.4

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  • 鼻腔ぬぐい液および抗原定量検査の臨床的有効性に関する検討

    藤谷 好弘, 黒沼 幸治, 高橋 聡

    感染症学雑誌   95 ( 臨増 )   251 - 251   2021.4

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  • 臨床応用を目指した肺胞微石症の基礎および治療法の検討

    齋藤 充史, 高宮 里奈, 藤谷 直樹, 有木 茂, 黒沼 幸治, 千葉 弘文, 高橋 素子, 高橋 弘毅

    日本肺サーファクタント・界面医学会雑誌   51   32 - 33   2021.3

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  • Characteristics of COVID-19 patients admitted into two hospitals in sapporo, Japan: Analyses and insights from two outbreak waves. Reviewed International journal

    Takeshi Hattori, Atsushi Saito, Hirofumi Chiba, Koji Kuronuma, Masaru Amishima, Daisuke Morinaga, Yasuo Shichinohe, Yasuyuki Nasuhara, Satoshi Konno

    Respiratory investigation   59 ( 2 )   180 - 186   2021.3

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    BACKGROUND: Coronavirus disease (COVID-19) emerged in January 2020 in Sapporo city, and the outbreak has shown two peaks. METHODS: A total of 260 COVID-19 patients were enrolled and categorized into three groups according to the pandemic pattern, jobs and situation, and disease severity. We compared clinical characteristics according to these categories. RESULTS: We found two pandemic peaks, and the proportion of patients and health providers who were infected in other hospitals had increased in the latter two periods (period 2: 49.6%, period 3: 32.7%). Particularly, the proportion of infected health providers was 27% in period 2, and they tended to be younger females with a mild condition. Severity of the disease (requirement of oxygen and/or mechanical ventilation) was associated with advanced age, and all the patients who died during admission were over 60 years old. CONCLUSIONS: We reported the temporal dynamics and characteristics of the COVID-19 pandemic in Sapporo city, Japan. This survey from the viewpoint of the hospital provides a new insight into and a better guide for the further management of the COVID-19 pandemic.

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  • 臨床応用を目指した肺胞微石症の基礎および治療法の検討

    齋藤 充史, 高宮 里奈, 藤谷 直樹, 有木 茂, 黒沼 幸治, 千葉 弘文, 高橋 素子, 高橋 弘毅

    日本肺サーファクタント・界面医学会雑誌   51   32 - 33   2021.3

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  • 肺多型癌に対しCBDCA+nab-PTX+Pembrolizumab療法を施行した3例

    村尾 公太郎, 小林 智史, 高橋 由紀乃, 石川 立, 浅井 悠一郎, 森 勇樹, 池田 貴美之, 高橋 守, 黒沼 幸治, 千葉 弘文

    肺癌   61 ( 1 )   64 - 64   2021.2

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  • Acute eosinophilic pneumonia accompanied with COVID-19: a case report. International journal

    Koutaro Murao, Atsushi Saito, Koji Kuronuma, Yoshihiro Fujiya, Satoshi Takahashi, Hirofumi Chiba

    Respirology case reports   8 ( 9 )   e00683   2020.12

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    We report a case of acute eosinophilic pneumonia (AEP) triggered by coronavirus disease 2019 (COVID-19) infection. A 77-year-old man experienced left-sided chest pain and shortness of breath. Reverse transcription-polymerase chain reaction (RT-PCR) for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) revealed a positive result, and he was treated with favipiravir, ciclesonide, and lascufloxacin, but he showed poor improvement. On the other hand, computed tomography (CT) images were atypical for COVID-19 infection, and the elevation of eosinophil was found in blood and the fluid obtained by bronchoscopy. So, we clinically diagnosed this case as AEP. Administration of prednisolone dramatically improved the patient's clinical condition and chest radiograph findings, which were consistent with the clinical course of AEP. This case suggests the importance of considering the complications of AEP when treating patients with COVID-19 infection.

    DOI: 10.1002/rcr2.683

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  • 高周波凝固法が有効であった多発気管・気管支乳頭腫の1例

    宮坂 友紀, 宮島 さつき, 関川 元基, 佐々原 正幸, 小玉 賢太郎, 斎藤 充史, 高橋 守, 黒沼 幸治, 千葉 弘文

    気管支学   42 ( 6 )   575 - 575   2020.11

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  • 嗅覚障害が持続する新型コロナウイルス感染症患者は退院後も経過に注意すべきである

    藤谷 好弘, 黒沼 幸治, 高橋 聡

    日本化学療法学会雑誌   68 ( Suppl.A )   302 - 302   2020.9

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  • 本邦における肺胞微石症の一症例とモデルマウスを用いた病態解析

    齋藤 充史, 高宮 里奈, 有木 茂, 黒沼 幸治, 千葉 弘文, 高橋 弘毅

    日本呼吸器学会誌   9 ( 増刊 )   254 - 254   2020.8

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  • 自己免疫疾患関連間質性肺炎のバイオマーカーの検討

    亀田 優美, 大塚 満雄, 長谷川 武宏, 吉田 真穂, 近藤 孝美, 黒沼 幸治, 千葉 弘文, 高橋 弘毅

    日本呼吸器学会誌   9 ( 増刊 )   294 - 294   2020.8

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  • 本邦における肺胞微石症の一症例とモデルマウスを用いた病態解析

    齋藤 充史, 高宮 里奈, 有木 茂, 黒沼 幸治, 千葉 弘文, 高橋 弘毅

    日本呼吸器学会誌   9 ( 増刊 )   254 - 254   2020.8

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  • Erratum: Surfactant protein A as a biomarker of outcomes of anti-fibrotic drug therapy in patients with idiopathic pulmonary fibrosis (BMC Pulm Med (2020) 20: 27 DOI: 10.1186/s12890-020-1060-y)

    Takumi Yoshikawa, Mitsuo Otsuka, Hirofumi Chiba, Kimiyuki Ikeda, Yuki Mori, Yasuaki Umeda, Hirotaka Nishikiori, Koji Kuronuma, Hiroki Takahashi

    BMC Pulmonary Medicine   20 ( 1 )   2020.5

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    DOI: 10.1186/s12890-020-1118-x

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  • Respiratory reactance in forced oscillation technique reflects disease stage and predicts lung physiology deterioration in idiopathic pulmonary fibrosis

    Yuki Mori, Hirotaka Nishikiori, Hirofumi Chiba, Gen Yamada, Koji Kuronuma, Hiroki Takahashi

    Respiratory Physiology and Neurobiology   275   2020.4

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    DOI: 10.1016/j.resp.2020.103386

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  • 肺MAC症における血清L-Ficolinの意義

    黒沼 幸治, 小林 智史, 斎藤 充史, 池田 貴美之, 大塚 満雄, 千葉 弘文, 有木 茂, 高橋 素子, 高橋 弘毅

    分子呼吸器病   24 ( 1 )   84 - 87   2020.3

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  • なるほど微生物学講座-不思議なミクロの世界- インフルエンザ菌におけるβ-ラクタムおよびキノロン系抗菌薬耐性に関する分子遺伝学的解析

    佐藤 豊孝, 本田 宏幸, 黒沼 幸治, 高橋 聡, 横田 伸一

    感染症学雑誌   94 ( 臨増 )   142 - 142   2020.3

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  • Haemophilus influenzaeのキノロン系抗菌薬耐性に関与する遺伝子変異の解析

    本田 宏幸, 佐藤 豊孝, 黒沼 幸治, 高橋 聡, 高橋 弘毅, 横田 伸一

    感染症学雑誌   94 ( 臨増 )   317 - 317   2020.3

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  • Surfactant protein A as a biomarker of outcomes of anti-fibrotic drug therapy in patients with idiopathic pulmonary fibrosis

    Takumi Yoshikawa, Mitsuo Otsuka, Hirofumi Chiba, Kimiyuki Ikeda, Yuki Mori, Yasuaki Umeda, Hirotaka Nishikiori, Koji Kuronuma, Hiroki Takahashi

    BMC Pulmonary Medicine   20 ( 1 )   2020.1

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    DOI: 10.1186/s12890-020-1060-y

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  • In Vitro Derivation of Fluoroquinolone-Resistant Mutants from Multiple Lineages of Haemophilus influenzae and Identification of Mutations Associated with Fluoroquinolone Resistance. Reviewed International journal

    Hiroyuki Honda, Toyotaka Sato, Masaaki Shinagawa, Yukari Fukushima, Chie Nakajima, Yasuhiko Suzuki, Koji Kuronuma, Satoshi Takahashi, Hiroki Takahashi, Shin-Ichi Yokota

    Antimicrobial agents and chemotherapy   64 ( 2 )   2020.1

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    Haemophilus influenzae is a pathogenic bacterium that causes respiratory and otolaryngological infections. The increasing prevalence of β-lactamase-negative high-level ampicillin-resistant H. influenzae (high-BLNAR) is a clinical concern. Fluoroquinolones are alternative agents to β-lactams. However, the emergence and increasing prevalence of fluoroquinolone-resistant H. influenzae have been reported. The current risk of fluoroquinolone resistance in H. influenzae (especially in high-BLNAR) has not yet been evaluated. Here, we examined the development of fluoroquinolone resistance in fluoroquinolone-susceptible clinical H. influenzae isolates in vitro during passaging in the presence of moxifloxacin (from 0.03 to 128 mg/liter). Twenty-nine isolates were examined. Seventeen isolates (58.6%) showed reduced moxifloxacin susceptibility, and 10 of these 17 isolates (34.5% of all isolates) exceeded the Clinical and Laboratory Standards Institute breakpoint for moxifloxacin (MIC of >1 mg/liter) after repeat cultivation on moxifloxacin-containing agar. Seven of these ten isolates were high-BLNAR and represented multiple lineages. We identified 56 novel mutations in 45 genes induced during the development of fluoroquinolone resistance, except the defined quinolone resistance-determining regions (Ser84Leu and Asp88Tyr/Gly/Asn in GyrA and Gly82Asp, Ser84Arg, and Glu88Lys in ParC). Glu153Leu and ΔGlu606 in GyrA, Ser467Tyr and Glu469Asp in GyrB, and ompP2 mutations were novel mutations contributing to fluoroquinolone resistance in H. influenzae In conclusion, H. influenzae clinical isolates from multiple lineages can acquire fluoroquinolone resistance by multiple novel mutations. The higher rate of derivation of fluoroquinolone-resistant H. influenzae from high-BLNAR than β-lactamase-negative ampicillin-susceptible isolates (P = 0.01) raises the possibility of the emergence and spread of fluoroquinolone-resistant high-BLNAR in the clinical setting.

    DOI: 10.1128/AAC.01500-19

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  • CXCL9, CXCL10, and CXCL11; biomarkers of pulmonary inflammation associated with autoimmunity in patients with collagen vascular diseases-associated interstitial lung disease and interstitial pneumonia with autoimmune features. International journal

    Masami Kameda, Mitsuo Otsuka, Hirofumi Chiba, Koji Kuronuma, Takehiro Hasegawa, Hiroki Takahashi, Hiroki Takahashi

    PloS one   15 ( 11 )   e0241719   2020

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    INTRODUCTION: Interstitial lung disease (ILD) is a heterogeneous group of diseases characterized by varying degrees of lung inflammation and/or fibrosis. We investigated biomarkers to infer whether patients with collagen vascular diseases associated ILD (CVD-ILD) and interstitial pneumonia with autoimmune features (IPAF) benefit from immunosuppressive therapy. MATERIALS AND METHODS: We retrospectively investigated patients with CVD-ILD, IPAF, and idiopathic pulmonary fibrosis (IPF) between June 2013 and May 2017 at our department. First, we assessed differences in serum and bronchoalveolar lavage fluid (BALF) levels of cytokines between groups. Second, we assessed the associations of patient's clinical variables with serum and BALF levels of those cytokines that were different between groups. Finally, we assessed the associations of diagnosis and response to immunosuppressive therapy with serum levels of those cytokines that were different between groups. RESULTS: We included 102 patients (51 with IPF, 35 with IPAF, and 16 with CVD-ILD). Serum and BALF levels of CXCL9, CXCL10, and CXCL11 were significantly elevated in patients with IPAF or CVD-ILD compared with those in patients with IPF. BALF levels of CXCL9 and CXCL10 were correlated with the percentages of lymphocytes and macrophages in BALF. Serum levels of CXCL9 and CXCL10 were correlated with BALF levels. Serum levels of CXCL9, CXCL10, and CXCL11 were correlated C-reactive protein, percent predicted forced vital capacity, alveolar-arterial oxygen difference, and the percentages of lymphocytes and macrophages in BALF. Serum levels of CXCL9, CXCL10, and CXCL11 showed moderate accuracy to distinguish patients with CVD-ILD from those with IPAF and IPF. Pre-treatment serum levels of CXCL9 and CXCL11 showed strong positive correlations with the annual forced vital capacity changes in patients with IPAF and CVD-ILD treated with immunosuppressive drugs. CONCLUSIONS: Serum CXCL9, CXCL10, and CXCL11 are potential biomarkers for autoimmune inflammation and predictors of the immunosuppressive therapy responses in ILD with background autoimmunity.

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  • IgG4関連疾患に合併したM.avium症の1例

    澤井 健之, 梅田 泰淳, 須藤 悠太, 浅井 悠一郎, 高橋 洋平, 小林 智史, 森 勇樹, 錦織 博貴, 黒沼 幸治, 山本 元久, 高橋 弘毅

    日本サルコイドーシス/肉芽腫性疾患学会雑誌   39 ( 1-2 )   113 - 113   2019.10

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  • 自己免疫性肺胞蛋白症の2例 症例を通して病態形成を考える

    池田 貴美之, 千葉 弘文, 萬谷 峻史, 澤井 健之, 齋藤 充史, 梅田 泰淳, 錦織 博貴, 黒沼 幸治, 大塚 満雄, 高橋 弘毅

    日本肺サーファクタント・界面医学会雑誌   50   48 - 50   2019.10

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  • Insufficient serum L-ficolin is associated with disease presence and extent of pulmonary Mycobacterium avium complex disease. Reviewed International journal

    Kobayashi T, Kuronuma K, Saito A, Ikeda K, Ariki S, Saitou A, Otsuka M, Chiba H, Takahashi S, Takahashi M, Takahashi H

    Respiratory research   20 ( 1 )   224 - 224   2019.10

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    BACKGROUND: The incidence of infectious disease caused by nontuberculous mycobacteria is increasing worldwide. Pulmonary Mycobacterium avium complex (MAC) disease is difficult to treat with chemotherapy, and its mechanism of infection, infection route, disease onset, and severity remain unknown. Ficolins are oligomeric defense lectins. L-ficolin plays an important role in innate immunity. This study's aim was to identify L-ficolin's role in patients with pulmonary MAC disease. METHODS: Between April 2011 and September 2017, 61 Japanese patients with pulmonary MAC disease were seen at our hospital. A control group, comprising 30 healthy individuals, without respiratory disease were enrolled in our study. The relationship between serum L-ficolin levels and disease severity was assessed, and L-ficolin's antibacterial role was examined. RESULTS: Serum L-ficolin levels were significantly lower in patients with pulmonary MAC disease than in healthy subjects (1.69 ± 1.27 μg/ml vs. 3.96 ± 1.42 μg/ml; p < 0.001). The cut-off value, based on receiver operating characteristic (ROC) analysis results, was 2.48 μg/ml (area under the curve (AUC) 0.90, sensitivity and specificity 83.6 and 86.7%, respectively). Serum L-ficolin levels were significantly lower in the patients with nodular bronchiectatic type disease compared with the patients with fibrocavitary type disease and were lower in the high-resolution computed tomography high-scoring group compared with low-scoring group. An in vitro analysis showed that purified recombinant L-ficolin bound to M. avium and its major cell wall component, lipoarabinomannan, in a concentration-dependent manner. In addition, recombinant L-ficolin suppressed M. avium growth in a concentration-dependent manner. CONCLUSIONS: Insufficient serum L-ficolin is associated with disease progression in pulmonary MAC disease, and the level of serum L-ficolin is a possible biomarker. TRIAL REGISTRATION: This study is registered with UMIN ( UMIN000022392 ).

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  • Autoimmune Pulmonary Alveolar Proteinosis Diagnosed after Exposure to a Fire Extinguisher Containing Silica Powder.

    Takafumi Yorozuya, Kimiyuki Ikeda, Hirofumi Chiba, Atsushi Saito, Koji Kuronuma, Hirotaka Nishikiori, Satsuki Miyajima, Mamoru Takahashi, Takumi Yoshikawa, Youhei Takahashi, Tetsuya Taya, Yuki Mori, Yasuaki Umeda, Mitsuo Otsuka, Hiroshi Moriyama, Hiroki Takahashi

    Internal medicine (Tokyo, Japan)   58 ( 14 )   2067 - 2072   2019.7

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    We herein report a case of autoimmune pulmonary alveolar proteinosis (PAP) diagnosed after one-time exposure to silica powder. Owing to the misuse of a silica-containing fire extinguisher and the inhalation of large amounts of its powder, the patient experienced prolonged cough and visited our hospital. The findings of chest computed tomography and surgical lung biopsy specimens led to the diagnosis of PAP. Interestingly, the presence of anti-GM-CSF antibody was detected; therefore, both autoimmune characteristics and exposure to large amounts of silica may have caused the development of PAP in this patient. This case provides important insight into the mechanisms leading to the onset of PAP.

    DOI: 10.2169/internalmedicine.1557-18

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  • 結核性膝関節炎を先行発症した結核性胸膜炎の1例

    小林 智史, 浅井 悠一朗, 池田 貴美之, 梅田 泰明, 亀田 優美, 錦織 博貴, 黒沼 幸治, 大塚 満雄, 千葉 弘文, 高橋 弘毅

    気管支学   41 ( Suppl. )   S355 - S355   2019.6

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  • 結核性胸膜炎を契機に診断しえた滑膜型結核性膝関節炎の1例

    小林 智史, 黒沼 幸治, 池田 貴美之, 錦織 博貴, 千葉 弘文, 高橋 弘毅

    結核   94 ( 6 )   395 - 400   2019.6

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  • 無症状で発見された気管支内過誤腫の一例

    梅田 泰淳, 浅井 悠一郎, 小林 智史, 池田 貴美之, 錦織 博貴, 高橋 守, 黒沼 幸治, 大塚 満雄, 千葉 弘文, 高橋 弘毅

    気管支学   41 ( Suppl. )   S282 - S282   2019.6

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  • Immunogenicity of pneumococcal vaccines in comorbid autoimmune and chronic respiratory diseases

    Koji Kuronuma, Hiroki Takahashi

    Human Vaccines and Immunotherapeutics   15 ( 4 )   859 - 862   2019.4

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    DOI: 10.1080/21645515.2018.1564443

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  • 肺MAC症患者におけるL-Ficolinの役割の検討

    小林 智史, 黒沼 幸治, 池田 貴美之, 錦織 博貴, 千葉 弘文, 高橋 弘毅

    結核   94 ( 3 )   251 - 251   2019.3

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  • 非結核性抗酸菌症 血清L-Ficolin低値が肺MAC症の発症に関連する

    小林 智史, 黒沼 幸治, 齋藤 充史, 池田 貴美之, 大塚 満雄, 千葉 弘文, 高橋 弘毅

    日本呼吸器学会誌   8 ( 増刊 )   158 - 158   2019.3

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  • 当院における迅速発育菌感染症の臨床的検討

    黒沼 幸治, 小林 智史, 池田 貴美之, 錦織 博貴, 千葉 弘文, 品川 雅明, 高橋 聡, 高橋 弘毅

    結核   94 ( 3 )   267 - 267   2019.3

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  • 骨髄線維症に対するJAK阻害薬使用中に結核性膝関節炎と結核性胸膜炎を発症した1例

    錦織 博貴, 小林 智史, 池田 貴美之, 黒沼 幸治, 千葉 弘文, 高橋 弘毅

    結核   94 ( 3 )   236 - 236   2019.3

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  • 血管内異物除去用スネアを用いて異物を除去した小児気管支異物の1例

    小玉 賢太郎, 長尾 喬生, 吉川 匠, 多屋 哲也, 池田 貴美之, 宮島 さつき, 高橋 守, 黒沼 幸治, 大塚 満雄, 千葉 弘文, 高橋 弘毅

    気管支学   41 ( 1 )   91 - 92   2019.1

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  • 結核性膝関節炎を先行発症した結核性胸膜炎の1例

    小林 智史, 本田 宏幸, 斎藤 充史, 錦織 博貴, 黒沼 幸治, 高橋 弘毅

    感染症学雑誌   93 ( 1 )   100 - 101   2019.1

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  • 特発性肺線維症における肺マイクロバイオームについての検討

    高橋 洋平, 齋藤 充史, 池田 貴美之, 小林 智史, 黒沼 幸治, 千葉 弘文, 佐々木 泰史, 高橋 弘毅

    日本サルコイドーシス/肉芽腫性疾患学会雑誌   38 ( 1-2 )   104 - 104   2018.10

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  • 特発性肺線維症における肺マイクロバイオーム研究の展開

    黒沼 幸治, 齋藤 充史, 池田 貴美之, 大塚 満雄, 千葉 弘文, 高橋 弘毅

    日本肺サーファクタント・界面医学会雑誌   49   1 - 7   2018.10

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  • Multiclonal Expansion and High Prevalence of β-Lactamase-Negative Haemophilus influenzae with High-Level Ampicillin Resistance in Japan and Susceptibility to Quinolones. Reviewed International journal

    Hiroyuki Honda, Toyotaka Sato, Masaaki Shinagawa, Yukari Fukushima, Chie Nakajima, Yasuhiko Suzuki, Tsukasa Shiraishi, Koji Kuronuma, Satoshi Takahashi, Hiroki Takahashi, Shin-Ichi Yokota

    Antimicrobial agents and chemotherapy   62 ( 9 )   2018.9

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    β-Lactam-resistant Haemophilus influenzae is a clinical concern. A high prevalence (>40%) of β-lactamase-negative high-level ampicillin-resistant H. influenzae (high-BLNAR) isolates in Japan has been reported. However, the reasons for the expansion are unknown. High-BLNAR strains possess an amino acid substitution, either Asn526Lys (group III) or Arg517His (group III-like) in addition to Ser385Thr, in penicillin-binding protein 3 (PBP3). To determine the current prevalence of high-BLNAR strains and the mechanisms behind their expansion in Japan, their prevalence, PBP3 types, multilocus sequence types, and susceptibilities to quinolones approved in Japan as alternatives were determined. Sixty percent of H. influenzae clinical isolates (62/104 isolates) were β-lactamase-negative ampicillin-resistant H. influenzae (BLNAR) strains. Among BLNAR isolates, 92% (57/62 isolates) were high-BLNAR strains. Most isolates were classified as belonging to group III, which contained many genotypes (11 PBP3 types and 25 sequence types). These results indicated that the expansion of high-BLNAR isolates was multiclonal and such strains are still predominant in Japanese clinical settings. One high-BLNAR isolate harbored the novel amino acid substitution Asn526Met in addition to Ser385Thr in PBP3, suggesting a new group (group IV). No quinolone-resistant H. influenzae isolates were identified. The MICs for the quinolones (moxifloxacin, garenoxacin, and tosufloxacin) were similar to that for levofloxacin, whereas sitafloxacin exhibited a lower MIC. However, we obtained 4 H. influenzae isolates with decreased quinolone susceptibility with the amino acid substitution Ser84Leu in GyrA, and 3 of those isolates were high-BLNAR isolates. In summary, this study shows that multiclonal high-BLNAR strains predominate in a Japanese university hospital. Isolates remain sensitive to quinolones, but vigilance is required to prevent the development of fluoroquinolone resistance in high-BLNAR strains.

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  • Contribution of Novel Amino Acid Alterations in PmrA or PmrB to Colistin Resistance in mcr-Negative Escherichia coli Clinical Isolates, Including Major Multidrug-Resistant Lineages O25b:H4-ST131-H30Rx and Non-x. International journal

    Toyotaka Sato, Tsukasa Shiraishi, Yoshiki Hiyama, Hiroyuki Honda, Masaaki Shinagawa, Masaru Usui, Koji Kuronuma, Naoya Masumori, Satoshi Takahashi, Yutaka Tamura, Shin-Ichi Yokota

    Antimicrobial agents and chemotherapy   62 ( 9 )   2018.9

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    Colistin is a last-line drug for multidrug-resistant Gram-negative bacteria. We previously reported four plasmid-mediated colistin resistance (mcr) gene-negative colistin-resistant Escherichia coli clinical isolates, including the major pathogenic and fluoroquinolone-resistant strains O25b:H4-ST131-H30Rx (isolates SRE34 and SRE44; MIC for colistin = 16 mg/liter), non-x (SME296; MIC = 8 mg/liter), and O18-ST416 (SME222; MIC = 4 mg/liter). In this study, we investigated the colistin resistance mechanism and identified novel amino acid substitutions or deletions in the PmrAB two-component system that activates eptA (encoding a phosphoethanolamine transferase) and arnT (encoding an undecaprenyl phosphate-alpha-4-amino-4-deoxy-l-arabinose arabinosyl transferase) in all colistin-resistant isolates. SRE34 possessed deletion Δ27-45 (LISVFWLWHESTEQIQLFE) in PmrB, SRE44 possessed substitution L105P in PmrA, and both SME222 and SME296 included substitution G206D in PmrB. Matrix-assisted laser desorption ionization-time of flight mass spectrometry revealed that lipid A is modified with phosphoethanolamine in all four isolates. Deletion of pmrAB decreased colistin MICs to 0.5 mg/liter and lowered eptA and arnT expression. Chromosomal replacement of mutated pmrA or pmrB in colistin-susceptible O25b:H4-ST131 strain SME98 (colistin MIC = 0.5 mg/liter) increased the colistin MIC to that of the respective parent colistin-resistant isolate. In addition, SME98 mutants in which pmrAB was replaced with mutated pmrAB showed no significant differences in bacterial growth and competition culture from the parent strain, except for the mutant with L105P in PmrA, whose growth was significantly suppressed in the presence of the parent strain. In conclusion, some O25b:H4-ST131 strains appear to acquire colistin resistance via phosphoethanolamine modification of lipid A through amino acid changes in PmrAB, and the amino acid changes in PmrB do not influence bacterial growth.

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  • Response to pneumococcal vaccine in interstitial lung disease patients: Influence of systemic immunosuppressive treatment. Reviewed International journal

    Koji Kuronuma, Hiroyuki Honda, Tessei Mikami, Atsushi Saito, Kimiyuki Ikeda, Mitsuo Otsuka, Hirofumi Chiba, Gen Yamada, Toyotaka Sato, Shin-Ichi Yokota, Hiroki Takahashi

    Vaccine   36 ( 33 )   4968 - 4972   2018.8

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    BACKGROUND: Interstitial lung diseases (ILD) are severe respiratory diseases, and ILD patients are treated with corticosteroid and immunosuppressive agents. However, it is unclear whether these medications influence the response of pneumococcal vaccine. OBJECTIVES: We examined the immunogenicity of pneumococcal vaccines (PPSV23 and PCV13) in ILD patients undergoing immunosuppressive treatment. METHODS: ILD patients who were regularly followed at the outpatient clinic were enrolled. Sera were collected before and 4-8 weeks after vaccination. Serotype-specific immunoglobulin G (IgG) concentrations against pneumococcal serotype 19F were measured by ELISA. RESULTS: IgG concentrations to serotype 19F were increased in all groups in response to the vaccine. Both PCV13 and PPSV23 induced IgG concentrations in patients immunized for the first time. Response rates for the ILD group were comparable with those for the ILD group undergoing corticosteroid therapy. Only idiopathic pulmonary fibrosis patients undergoing immunosuppressive therapy had a significantly lower response.

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  • Whole genome analysis of a multidrug-resistant Streptococcus pneumoniae isolate from a patient with invasive pneumococcal infection developing disseminated intravascular coagulation. International journal

    Yasuo Ohkoshi, Toyotaka Sato, Takayuki Wada, Yukari Fukushima, Hiromi Murabayashi, Yasunari Takakuwa, Kaoru Nishiyama, Hiroyuki Honda, Tsukasa Shiraishi, Koji Kuronuma, Hiroki Takahashi, Chie Nakajima, Yasuhiko Suzuki, Shin-Ichi Yokota

    Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy   24 ( 8 )   674 - 681   2018.8

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    Multidrug-resistant Streptococcus pneumoniae strains were isolated from blood and sputum of a patient with disseminated intravascular coagulation in Sapporo city, Japan. These antibiograms were only susceptible to vancomycin, linezolid, daptomycin, some carbapenems, and some fluoroquinolones. Identical antibiograms, serotypes (19F), and sequence types (ST10017) suggested a shared origin of these isolates. Only one ST10017 strain has been isolated in the same city in Japan previously (2014), and the 2014 isolate is still susceptible to macrolides. The whole genome of the blood-derived isolate was sequenced. The strain harbored resistance mutations in parC, gyrA, pbp1a, pbp2a, pbp2b, and pbp2x, and harbored the resistance genes, ermB and tetM. The nucleotide sequences of parC and pbp2x genes of strain MDRSPN001 were clearly different from those of other S. pneumoniae strains and were similar to those of oral streptococci strains. These findings suggest that strain MDRSPN001 has been rapidly and drastically evolving multidrug resistance by gene replacement and accumulation of genes originating from other strains, such as oral streptococci, Streptococcus mitis.

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  • 肺MAC症患者におけるL-Ficolinの役割の検討

    小林 智史, 齋藤 充史, 本田 宏幸, 錦織 博貴, 黒沼 幸治, 高橋 弘毅

    日本化学療法学会雑誌   66 ( Suppl.A )   347 - 347   2018.4

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  • 侵襲性肺炎球菌感染症患者から分離された多剤耐性肺炎球菌の解析

    本田 宏幸, 佐藤 豊孝, 大越 康雄, 村林 広美, 小林 智史, 齋藤 充史, 錦織 博貴, 黒沼 幸治, 高橋 弘毅, 横田 伸一

    日本化学療法学会雑誌   66 ( Suppl.A )   289 - 289   2018.4

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  • 結核感染を合併したStreptococcus salivariusおよびStreptococcus mitis/oralisによる肺膿瘍の1例

    小林 智史, 本田 宏幸, 斎藤 充史, 錦織 博貴, 黒沼 幸治, 高橋 弘毅

    感染症学雑誌   92 ( 2 )   229 - 230   2018.3

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  • EGFR遺伝子変異陽性肺腺癌患者における予後予測因子としてのSP-Dの有用性

    梅田 泰淳, 長谷川 喜弘, 大塚 満雄, 黒沼 幸治, 高橋 素子, 高橋 弘毅

    日本内科学会雑誌   107 ( Suppl. )   176 - 176   2018.2

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  • Impaired diversity of the lung microbiome predicts progression of idiopathic pulmonary fibrosis. Reviewed

    Takahashi Y, Saito A, Chiba H, Kuronuma K, Ikeda K, Kobayashi T, Ariki S, Takahashi M, Sasaki Y, Takahashi H

    Respiratory research   19 ( 1 )   34   2018.2

  • Poorer Prognosis of Idiopathic Pleuroparenchymal Fibroelastosis Compared with Idiopathic Pulmonary Fibrosis in Advanced Stage

    Makoto Shioya, Mitsuo Otsuka, Gen Yamada, Yasuaki Umeda, Kimiyuki Ikeda, Hirotaka Nishikiori, Koji Kuronuma, Hirofumi Chiba, Hiroki Takahashi

    Canadian Respiratory Journal   2018   2018

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    DOI: 10.1155/2018/6043053

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  • Surfactant protein D inhibits activation of non-small cell lung cancer-associated mutant EGFR and affects clinical outcomes of patients

    Y. Umeda, Y. Hasegawa, M. Otsuka, S. Ariki, R. Takamiya, A. Saito, Y. Uehara, H. Saijo, K. Kuronuma, H. Chiba, H. Ohnishi, Y. Sakuma, H. Takahashi, Y. Kuroki, M. Takahashi

    Oncogene   36 ( 46 )   6432 - 6445   2017.11

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    DOI: 10.1038/onc.2017.253

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  • Surfactant protein A down-regulates epidermal growth factor receptor by mechanisms different from those of surfactant protein D. International journal

    Yoshihiro Hasegawa, Motoko Takahashi, Shigeru Ariki, Atsushi Saito, Yasuaki Uehara, Rina Takamiya, Koji Kuronuma, Hirofumi Chiba, Yuji Sakuma, Hiroki Takahashi, Yoshio Kuroki

    The Journal of biological chemistry   292 ( 45 )   18565 - 18576   2017.11

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    We recently reported that the lectin surfactant protein D (SP-D) suppresses epidermal growth factor receptor (EGFR) signaling by interfering with ligand binding to EGFR through an interaction between the carbohydrate-recognition domain (CRD) of SP-D and N-glycans of EGFR. Here, we report that surfactant protein A (SP-A) also suppresses EGF signaling in A549 human lung adenocarcinoma cells and in CHOK1 cells stably expressing human EGFR and that SP-A inhibits the proliferation and motility of the A549 cells. Results with 125I-EGF indicated that SP-A interferes with EGF binding to EGFR, and a ligand blot analysis suggested that SP-A binds EGFR in A549 cells. We also found that SP-A directly binds the recombinant extracellular domain of EGFR (soluble EGFR or sEGFR), and this binding, unlike that of SP-D, was not blocked by EDTA, excess mannose, or peptide:N-glycosidase F treatment. We prepared a collagenase-resistant fragment (CRF) of SP-A, consisting of CRD plus the neck domain of SP-A, and observed that CRF directly binds sEGFR but does not suppress EGF-induced phosphorylation of EGFR in or proliferation of A549 cells. These results indicated that SP-A binds EGFR and down-regulates EGF signaling by inhibiting ligand binding to EGFR as well as SP-D. However, unlike for SP-D, SP-A lectin activity and EGFR N-glycans were not involved in the interaction between SP-A and EGFR. Furthermore, our results suggested that oligomerization of SP-A is necessary to suppress the effects of SP-A on EGF signaling.

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  • Serum surfactant protein D predicts the outcome of patients with idiopathic pulmonary fibrosis treated with pirfenidone Reviewed

    Kimiyuki Ikeda, Masanori Shiratori, Hirofumi Chiba, Hirotaka Nishikiori, Keiki Yokoo, Atsushi Saito, Yoshihiro Hasegawa, Koji Kuronuma, Mitsuo Otsuka, Gen Yamada, Hiroki Takahashi

    RESPIRATORY MEDICINE   131   184 - 191   2017.10

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    DOI: 10.1016/j.rmed.2017.08.021

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  • Deterioration of regulatory B cells and activation of follicular helper T cells in idiopathic interstitial pneumonias patients Reviewed

    Asai Yuichiro, Chiba Hirofumi, Kondoh Syun, Nishikiori Hirotaka, Takahashi Mamoru, Kuronuma Koji, Otsuka Mitsuo, Yamada Gen, Kamekura Ryuta, Ichimiya Shingo, Takahashi Hiroki

    EUROPEAN RESPIRATORY JOURNAL   50   2017.9

  • 高周波スネアによる腫瘍切除術を施行した気管支発生の炎症性筋線維芽細胞腫瘍の1例

    浅井 悠一郎, 梅田 泰淳, 黒沼 幸治, 横山 早織, 近藤 瞬, 須藤 悠太, 小林 智史, 亀田 優美, 錦織 博貴, 千葉 弘文, 山田 玄, 高橋 弘毅

    気管支学   39 ( 5 )   386 - 391   2017.9

  • Volatile Organic Compounds in Exhaled Breath of Idiopathic Pulmonary Fibrosis for Discrimination from Healthy Subjects Reviewed

    Yu-ichi Yamada, Gen Yamada, Mitsuo Otsuka, Hirotaka Nishikiori, Kimiyuki Ikeda, Yasuaki Umeda, Hirofumi Ohnishi, Koji Kuronuma, Hirofumi Chiba, Joerg Ingo Baumbach, Hiroki Takahashi

    LUNG   195 ( 2 )   247 - 254   2017.4

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    DOI: 10.1007/s00408-017-9979-3

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  • Surfactant protein A (SP-A) and SP-A-derived peptide attenuate chemotaxis of mast cells induced by human β-defensin 3. International journal

    Yasuaki Uehara, Motoko Takahashi, Masaki Murata, Atsushi Saito, Rina Takamiya, Yoshihiro Hasegawa, Koji Kuronuma, Hirofumi Chiba, Jiro Hashimoto, Norimasa Sawada, Hiroki Takahashi, Yoshio Kuroki, Shigeru Ariki

    Biochemical and biophysical research communications   485 ( 1 )   107 - 112   2017.3

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    Human β-defensin 3 (hBD3) is known to be involved in mast cell activation. However, molecular mechanisms underlying the regulation of hBD3-induced mast cell activation have been poorly understood. We previously reported that SP-A and SP-A-derived peptide 01 (SAP01) regulate the function of hBD3. In this study, we focused on the effects of SP-A and SAP01 on the activation of mast cells induced by hBD3. SAP01 directly bound to hBD3. Mast cell-mediated vascular permeability and edema in hBD3 administered rat ears were decreased when injected with SP-A or SAP01. Compatible with the results in rat ear model, both SP-A and SAP01 inhibited hBD3-induced chemotaxis of mast cells in vitro. Direct interaction between SP-A or SAP01 and hBD3 seemed to be responsible for the inhibitory effects on chemotaxis. Furthermore, SAP01 attenuated hBD3-induced accumulation of mast cells and eosinophils in tracheas of the OVA-sensitized inflammatory model. SP-A might contribute to the regulation of inflammatory responses mediated by mast cells during infection.

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  • 非HIV患者におけるST合剤使用困難ニューモシスチス肺炎に対するアトバコンの使用経験

    小林 智史, 齊藤 充史, 錦織 博貴, 黒沼 幸治, 高橋 弘毅

    感染症学雑誌   91 ( 1 )   93 - 93   2017.1

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  • 【肺炎への最新アプローチ-ジェネラリストの立場とスペシャリストの視点から】治療 その他の治療薬や管理 高齢者肺炎への対応 インフルエンザワクチンと肺炎球菌ワクチンの最近の考え方 プレベナーとニューモバックス

    黒沼 幸治, 本田 宏幸

    Medicina   54 ( 1 )   106 - 108   2017.1

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  • Treatment of pulmonary fibrosis with siRNA against a collagen-specific chaperone HSP47 in vitamin A-coupled liposomes Reviewed

    Mitsuo Otsuka, Masanori Shiratori, Hirofumi Chiba, Koji Kuronuma, Yasushi Sato, Yoshiro Niitsu, Hiroki Takahashi

    EXPERIMENTAL LUNG RESEARCH   43 ( 6-7 )   271 - 282   2017

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    DOI: 10.1080/01902148.2017.1354946

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  • Congenital Pulmonary Vein Atresia in an Adult.

    Koji Kuronuma, Tomofumi Kobayashi, Youhei Takahashi, Hiroki Takahashi

    Internal medicine (Tokyo, Japan)   56 ( 7 )   877 - 878   2017

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    DOI: 10.2169/internalmedicine.56.7590

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  • Validation of the Japanese disease severity classification and the GAP model in Japanese patients with idiopathic pulmonary fibrosis Reviewed

    Shun Kondoh, Hirofumi Chiba, Hirotaka Nishikiori, Yasuaki Umeda, Koji Kuronuma, Mitsuo Otsuka, Gen Yamada, Hirofumi Ohnishi, Mitsuru Mori, Yasuhiro Kondoh, Hiroyuki Taniguchi, Sakae Homma, Hiroki Takahashi

    Respiratory Investigation   54 ( 5 )   327 - 333   2016.9

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    DOI: 10.1016/j.resinv.2016.02.009

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  • Surfactant protein D is a useful biomarker for monitoring acute lung injury in rats

    Makoto Murata, Mitsuo Otsuka, Noriyuki Ashida, Gen Yamada, Koji Kuronuma, Hirofumi Chiba, Hiroki Takahashi

    Experimental Lung Research   42 ( 6 )   314 - 321   2016.7

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    DOI: 10.1080/01902148.2016.1215570

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  • 免疫グロブリン製剤補充により改善を認めた原発性免疫不全症に伴う気管支拡張症の1例

    小林 智史, 錦織 博貴, 黒沼 幸治, 高橋 弘毅

    感染症学雑誌   90 ( 3 )   398 - 399   2016.5

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  • 高周波スネアによる気管支鏡下腫瘍摘出術を施行した気管支原発炎症性筋線維芽腫瘍の一例

    黒沼 幸治, 梅田 泰淳, 横山 早織, 高橋 知之, 柳 昌弘, 近藤 瞬, 浅井 悠一郎, 小林 智史, 汐谷 心, 亀田 優美, 錦織 博貴, 大塚 満雄, 千葉 弘文, 山田 玄, 高橋 弘毅

    気管支学   38 ( Suppl. )   S309 - S309   2016.5

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  • 北海道における成人侵襲性肺炎球菌感染症症例のサーベイランス

    黒沼 幸治, 小林 智史, 錦織 博貴, 常 彬, 大石 和徳, 高橋 弘毅

    感染症学雑誌   90 ( 3 )   376 - 376   2016.5

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  • 内視鏡・画像診断 気管支鏡検査における呼吸器内科医による迅速細胞診の有用性の検討

    梅田 泰淳, 大塚 満雄, 小林 智史, 汐谷 心, 池田 貴美之, 亀田 優美, 黒沼 幸治, 高橋 守, 山田 玄, 高橋 弘毅

    日本呼吸器学会誌   5 ( 増刊 )   162 - 162   2016.3

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  • SP-AおよびSP-A由来ペプチドはヒトβ-デフェンシン3による肥満細胞の遊走を抑制する

    有木 茂, 上原 康昭, 村田 雅樹, 高宮 里奈, 長谷川 喜弘, 斎藤 充史, 千葉 弘文, 黒沼 幸治, 高橋 素子, 高橋 弘毅, 黒木 由夫

    分子呼吸器病   20 ( 1 )   120 - 123   2016.3

  • [THE CURRENT SITUATION OF FOREIGN TUBERCULOSIS PATIENTS AND THEIR CONCURRENT HIV INFECTION IN HOKKAIDO].

    Kimiyuki Ikeda, Hirotaka Nishikiori, Shun Kondo, Tomofumi Kobayashi, Tetsuya Taya, Yuki Mori, Makoto Shioya, Koji Kuronuma, Hiroki Takahashi

    Kekkaku : [Tuberculosis]   91 ( 2 )   33 - 9   2016.2

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    BACKGROUND AND PURPOSE: According to recent news, patients with concurrent tuberculosis (TB) and human immunodeficiency virus (HIV) infection are increasingly common worldwide. This study aimed to investigate whether TB/HIV co-infected patients are visiting Hokkaido. METHOD: We conducted a questionnaire survey regarding foreign patients infected with TB or TB/HIV who visited Hokkaido between January 2001 and September 2014. We mailed questionnaires to health centers, AIDS treatment care hospitals, and TB hospitals in Hokkaido prefecture. RESULTS: Seventy-one TB patients were of foreign nationality according to the answers obtained from health centers. Most of them were foreign students or occupational trainees between 20-30 years old. Approximately half these patients were from East Asia, and 7 patients were from Africa. As 21 % of the patients with TB who visited medical examination were over 1 month from disease onset, and the delay in visiting was recognized. The TB infection was mostly detected coincidentally during the physician visit. In the hospital survey, four TB patients with HIV were of foreign nationality. They were also of the age group from 20-30 years and hailed from sub-Saharan Africa. DISCUSSION: During immigration, medical examination by performing a chest radiograph is important. If the immigrant hails from an area where TB and HIV co-infection is common, it is necessary to confirm whether HIV infection is present.

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  • 北海道における外国籍結核患者とそのHIV感染症合併の現状

    池田 貴美之, 錦織 博貴, 近藤 瞬, 小林 智史, 多屋 哲也, 森 勇樹, 汐谷 心, 黒沼 幸治, 高橋 弘毅

    結核   91 ( 2 )   33 - 39   2016.2

  • 気管支内腔に発生した炎症性筋線維芽腫瘍に対し高周波スネアによる気管支鏡下腫瘍摘出術を施行した1例

    横山 早織, 高橋 知之, 柳 昌弘, 近藤 瞬, 浅井 悠一郎, 小林 智史, 梅田 泰淳, 汐谷 心, 亀田 優美, 錦織 博貴, 黒沼 幸治, 千葉 弘文, 山田 玄, 高橋 弘毅

    気管支学   38 ( 1 )   55 - 55   2016.1

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  • A Case of Recurrent Cryptogenic Organizing Pneumonia Complicated by Primary Pulmonary Malignant Lymphoma

    Umeda Yasuaki, Otsuka Mitsuo, Kuronuma Koji, Kodera Yuki, Kondo Shun, Taya Tetsuya, Ikeda Kimiyuki, Shioya Makoto, Chiba Hirofumi, Yamada Gen, Takahashi Hiroki

    The Journal of the Japan Society for Respiratory Endoscopy   38 ( 2 )   134 - 139   2016

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    Background. Cryptogenic organizing pneumonia (COP) usually responds well to corticosteroid treatment and typically has a good prognosis. However, a few cases are refractory to corticosteroid treatment. Immunosuppressant drugs are associated with an increased risk of malignant lymphoproliferative disorders. Case. A 63-year-old man presented with a history of cough for a month. High resolution computed tomography (HRCT) of the chest showed air-space consolidation predominantly in both lungs. We diagnosed COP by transbronchial lung biopsy (TBLB) specimens. Clinical data and HRCT findings were improved by prednisolone therapy, but recurrent exacerbation occurred during steroid tapering. Although, we re-examined the abnormal lesions in the left lower lobe by video-assisted thoracoscopy and those in the right lower lobe by TBLB, histopathological examinations of all lesions revealed organizing pneumonia. We continued immunosuppressive therapy with a combination of prednisolone and immunosuppressive agents. However, lung lesions gradually deteriorated. In order to obtain a histopathological diagnosis again, we performed TBLB from the right lower lobe. Histopathological examination revealed lung invasion of diffuse large B cell type lymphoma. Conclusion. We experienced a rare case of recurrent COP complicated by primary pulmonary malignant lymphoma. It is necessary to pay attention to the possibility that malignant lymphoma may be involved in recurrent COP during immunosuppressive therapy.

    Other Link: http://search.jamas.or.jp/link/ui/2016212248

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  • IgG補充療法により改善を認めた原発性免疫不全症に伴う気管支拡張症の1例

    小林 智史, 黒沼 幸治, 高橋 弘毅

    Therapeutic Research   36 ( 6 )   557 - 558   2015.6

  • 呼吸器内科医による気管支鏡検査における迅速細胞診の有用性の検討

    梅田 泰淳, 大塚 満雄, 小林 智史, 汐谷 心, 池田 貴美之, 亀田 優美, 成田 欣史, 北田 順也, 黒沼 幸治, 高橋 守, 山田 玄, 高橋 弘毅

    気管支学   37 ( Suppl. )   S290 - S290   2015.5

  • 続発性難治性気胸に対しEWSを用いて気管支充填術を施行した7症例の検討

    小林 智史, 黒沼 幸治, 梅田 泰淳, 汐谷 心, 成田 欣史, 亀田 優美, 北田 順也, 大塚 満雄, 千葉 弘文, 山田 玄, 高橋 弘毅

    気管支学   37 ( Suppl. )   S247 - S247   2015.5

  • 血痰を契機に発見された心房細動カテーテルアブレーション後の肺静脈狭窄症の1例

    夏井坂 元基, 亀田 優美, 大塚 満雄, 黒沼 幸治, 北田 順也, 成田 欣史, 梅田 泰淳, 横尾 慶紀, 千葉 弘文, 山田 玄, 高橋 弘毅

    気管支学   37 ( 1 )   87 - 93   2015.1

  • Distinct compartmentalization of SP-A and SP-D in the vasculature and lungs of patients with idiopathic pulmonary fibrosis Reviewed

    Hirotaka Nishikiori, Hirofumi Chiba, Shigeru Ariki, Koji Kuronuma, Mitsuo Otsuka, Masanori Shiratori, Kimiyuki Ikeda, Atsushi Watanabe, Yoshio Kuroki, Hiroki Takahashi

    BMC PULMONARY MEDICINE   14   196   2014.12

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    DOI: 10.1186/1471-2466-14-196

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  • 気管内挿管後の炎症性肉芽腫性閉塞に対し気管支鏡下電気焼灼術(高周波スネア)が有効であった1例

    田中 優子, 宮島 さつき, 森 勇樹, 梅田 泰淳, 汐谷 心, 成田 欣史, 亀田 優美, 北田 順也, 黒沼 幸治, 千葉 弘文, 山田 玄, 高橋 弘毅

    気管支学   36 ( 6 )   681 - 682   2014.11

  • PZA、LVFX、TH、KMによる治療が奏効した多剤耐性肺結核の1例

    本田 宏幸, 森 勇樹, 梅田 泰淳, 汐谷 心, 成田 欣史, 亀田 優美, 北田 順也, 黒沼 幸治, 千葉 弘文, 山田 玄, 高橋 弘毅

    結核   89 ( 5 )   588 - 588   2014.5

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  • 血痰で発見されたカテーテルアブレーション後肺静脈狭窄症の1例

    亀田 優美, 黒沼 幸治, 夏井坂 元基, 梅田 泰淳, 成田 欣史, 北田 順也, 大塚 満雄, 千葉 弘文, 山田 玄, 高橋 弘毅

    気管支学   36 ( Suppl. )   S216 - S216   2014.3

  • 111In骨髄シンチグラフィが治療効果判定に有用だった肺小細胞癌・骨髄転移の1例

    森 勇樹, 亀田 優美, 小寺 祐貴, 斎藤 淳, 梅田 泰淳, 汐谷 心, 成田 欣史, 北田 順也, 黒沼 幸治, 大塚 満雄, 千葉 弘文, 山田 玄, 高橋 弘毅

    肺癌   53 ( 7 )   917 - 917   2013.12

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  • 血清SP-D異常低値を呈する肺癌症例の検討

    成田 欣史, 黒沼 幸治, 近藤 瞬, 梅田 泰淳, 汐谷 心, 亀田 優美, 北村 康夫, 北田 順也, 大塚 満雄, 千葉 弘文, 山田 玄, 高橋 弘毅

    日本呼吸器学会誌   2 ( 増刊 )   230 - 230   2013.3

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  • 当科における最近の肺非結核性抗酸菌症の臨床的検討

    亀田 優美, 山田 玄, 黒沼 幸治, 大塚 満雄, 千葉 弘文, 高橋 弘毅

    日本呼吸器学会誌   2 ( 増刊 )   216 - 216   2013.3

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  • Detection of N-glycolyated gangliosides in non-small-cell lung cancer using GMR8 monoclonal antibody. Reviewed International journal

    Nobuyoshi Hayashi, Hirofumi Chiba, Koji Kuronuma, Shinji Go, Yoshihiro Hasegawa, Motoko Takahashi, Shinsei Gasa, Atsushi Watanabe, Tadashi Hasegawa, Yoshio Kuroki, Jinichi Inokuchi, Hiroki Takahashi

    Cancer science   104 ( 1 )   43 - 7   2013.1

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    Gangliosides are glycosphingolipids found on the cell surface. They act as recognition molecules or signal modulators and regulate cell proliferation and differentiation. N-glycolylneuraminic acid (NeuGc)-containing gangliosides have been detected in some neoplasms in humans, although they are usually absent in normal human tissues. Our aim was to evaluate the presence of NeuGc-containing gangliosides including GM3 (NeuGc) and assess their relationship with the prognosis of non-small-cell lung cancer (NSCLC). NeuGc-containing ganglioside expression in NSCLC tissues was analyzed immunohistochemically using the mouse monoclonal antibody GMR8, which is specific for gangliosides with NeuGc alpha 2,3Gal-terminal structures. On the basis of NeuGc-containing ganglioside expression, we performed survival analysis. We also investigated the differences in the effects of GM3 (N-acetylneuraminic acid [NeuAc]) and GM3 (NeuGc) on inhibition of epidermal growth factor receptor (EGFR) tyrosine kinase in A431 cells. As a result, the presence of NeuGc-containing gangliosides was evident in 86 of 93 (93.5%) NSCLC samples. The NSCLC patients with high NeuGc-containing ganglioside expression had a low overall survival rate and a significantly low progression-free survival rate. In the in vitro study, the inhibitory effect of GM3 on EGFR tyrosine kinase in A431 cells after exposure to GM3 (NeuGc) was lower than that after exposure to GM3 (NeuAc). In conclusion, NeuGc-containing gangliosides including GM3 (NeuGc) are widely expressed in NSCLC, and NeuGc-containing ganglioside expression is associated with patient survival. The difference in the effects of GM3 (NeuGc) and GM3 (NeuAc) on the inhibition of EGFR tyrosine kinase might contribute to improvement in the prognosis of NSCLC patients.

    DOI: 10.1111/cas.12027

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  • エンドサイトスコピーを用いた腫瘍性病変の観察

    池田 貴美之, 山田 裕一, 梅田 泰淳, 亀田 優美, 成田 欣史, 錦織 博貴, 宮島 さつき, 北田 順也, 黒沼 幸治, 大塚 満雄, 山田 玄, 高橋 弘毅

    肺癌   52 ( 5 )   788 - 788   2012.10

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  • ゲフィチニブ投与後にアルカリフォスファターゼ・フレア現象を呈した肺腺癌の2例

    亀田 優美, 梅田 泰淳, 汐谷 心, 成田 欣史, 北村 康夫, 北田 順也, 宮島 さつき, 黒沼 幸治, 大塚 満雄, 山田 玄, 高橋 弘毅

    肺癌   52 ( 5 )   793 - 793   2012.10

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  • Pulmonary surfactant protein A protects lung epithelium from cytotoxicity of human β-defensin 3. Reviewed

    Saito A, Ariki S, Sohma H, Nishitani C, Inoue K, Ebata N, Takahashi M, Hasegawa Y, Kuronuma K, Takahashi H, Kuroki Y

    The Journal of biological chemistry   287 ( 18 )   15034 - 15043   2012.4

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  • A Case of Adult T Cell Leukemia/Lymphoma Presenting as Severe Tracheal Stenosis Reviewed

    Kengo Otsuka, Koji Kuronuma, Mitsuo Otsuka, Junya Kitada, Yoshifumi Narita, Hirotoshi Homma, Satsuki Miyajima, Yasuo Kitamura, Yosuke Suzuki, Motoki Natsuizaka, Hiroshi Yasui, Hiroshi Ikeda, Toshiaki Hayashi, Tadao Ishida, Yasuhisa Shinomura, Hiroko Noguchi, Hiroki Takahashi

    INTERNAL MEDICINE   50 ( 21 )   2637 - 2641   2011

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    DOI: 10.2169/internalmedicine.50.5930

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  • ゲフィチニブ奏効後耐性化した肺腺癌に対してエルロチニブへの変更が有効であった当院における経験例

    角 俊行, 成田 欣史, 北村 康夫, 北田 順也, 本間 裕敏, 黒沼 幸治, 大塚 満雄, 工藤 和実, 村上 聖司, 山田 玄, 田中 裕士, 高橋 弘毅

    肺癌   50 ( 7 )   946 - 947   2010.12

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  • Pulmonary collectins enhance phagocytosis of Mycobacterium avium through increased activity of mannose receptor. Reviewed

    Kudo K, Sano H, Takahashi H, Kuronuma K, Yokota S, Fujii N, Shimada K, Yano I, Kumazawa Y, Voelker DR, Abe S, Kuroki Y

    Journal of Immunology   172 ( 12 )   7592 - 602   2004

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  • Two cases of extrapulmonary tuberculosis with delayed diagnosis

    小林拓海, 齋藤充史, 小玉賢太郎, 高橋守, 黒沼幸治, 千葉弘文

    日本呼吸器学会誌(Web)   13   2024

  • Metabolic activity of alveolar macrophages in bronchoalveolar lavage fluid in interstitial lung disease

    萬谷峻史, 齋藤充史, 佐藤達也, 山口美樹, 亀田優美, 佐久間裕司, 黒沼幸治, 千葉弘文

    日本呼吸器学会誌(Web)   13   2024

  • 手洗器の水栓口や給水システムの汚染による緑膿菌の感染伝播

    藤谷好弘, 中江舞美, 佐藤勇樹, 韮澤慎也, 富樫篤生, 富樫篤生, 齋藤充史, 齋藤充史, 黒沼幸治, 黒沼幸治, 高橋聡, 高橋聡, 高橋聡

    日本感染症学会東日本地方会学術集会・日本化学療法学会東日本支部総会合同学会プログラム・抄録集   72nd-70th   2023

  • Klebsiella pneumoniaeにおける遺伝子変異頻度と病原性の関連性評価

    上村幸二朗, 上村幸二朗, 佐藤豊孝, 佐藤豊孝, 佐藤豊孝, 黒沼幸治, 黒沼幸治, 高橋聡, 高橋聡, 横田伸一

    日本感染症学会東日本地方会学術集会・日本化学療法学会東日本支部総会合同学会プログラム・抄録集   71st-69th   2022

  • 新型コロナウイルス感染症が北海道内の病院・診療所の診療に与えた影響に関するアンケート調査

    齋藤 充史, 千葉 弘文, 服部 健史, 黒沼 幸治, 中久保 祥, 鎌田 啓佑, 田代 典夫, 田中 裕士, 福家 聡, 今野 哲

    日本呼吸器学会誌   10 ( 3 )   245 - 250   2021.5

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  • マイクロバイオーム解析に基づく間質性肺炎の病態解明

    齋藤充史, 齋藤充史, 黒沼幸治, 小林智史, 本田宏幸, 錦織博貴, 高橋弘毅

    日本化学療法学会雑誌   66 ( Supplement-A )   321   2018.4

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  • 免疫抑制療法中の間質性肺疾患患者における肺炎球菌ワクチンの効果の検討

    黒沼幸治, 本田宏幸, 本田宏幸, 齋藤充史, 小林智史, 錦織博貴, 佐藤豊孝, 横田伸一, 高橋弘毅

    日本化学療法学会雑誌   66 ( Supplement-A )   378   2018.4

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  • SP-Dによる変異型EGFR肺がんの制御機構

    長谷川 喜弘, 梅田 泰淳, 大塚 満雄, 有木 茂, 高宮 里奈, 齋藤 充史, 黒沼 幸治, 千葉 弘文, 高橋 弘毅, 黒木 由夫, 高橋 素子

    分子呼吸器病   21 ( 1 )   80 - 83   2017.3

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  • SP-Dによる変異型EGFR肺がんの制御機構

    長谷川 喜弘, 梅田 泰淳, 大塚 満雄, 有木 茂, 高宮 里奈, 齋藤 充史, 黒沼 幸治, 千葉 弘文, 高橋 弘毅, 黒木 由夫, 高橋 素子

    分子呼吸器病   21 ( 1 )   80 - 83   2017.3

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  • 多発縦隔リンパ節腫脹を認め、EUS-FNAにより診断したMycobacterium avium症の1例

    黒沼 幸治, 小林 智史, 齋藤 充史, 錦織 博貴, 高橋 弘毅

    感染症学雑誌   91 ( 1 )   98 - 98   2017.1

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  • 急速な胸水貯留を伴った胸壁原発血管腫の1例

    池田 貴美之, 多屋 哲也, 齋藤 充史, 高橋 守, 黒沼 幸治, 大塚 満雄, 千葉 弘文, 白鳥 正典, 山田 玄, 高橋 弘毅

    気管支学   38 ( Suppl. )   S278 - S278   2016.5

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  • SP-AおよびSP-A由来ペプチドはヒトβ-デフェンシン3による肥満細胞の遊走を抑制する

    有木 茂, 上原 康昭, 村田 雅樹, 高宮 里奈, 長谷川 喜弘, 斎藤 充史, 千葉 弘文, 黒沼 幸治, 高橋 素子, 高橋 弘毅, 黒木 由夫

    分子呼吸器病   20 ( 1 )   120 - 123   2016.3

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  • SP-DによるEGFR遺伝子変異陽性肺腺癌における制御機構

    梅田泰淳, 長谷川喜弘, 長谷川喜弘, 大塚満雄, 黒沼幸治, 高橋素子, 高橋弘毅

    日本肺サーファクタント・界面医学会学術研究会プログラム・抄録集   52nd   2016

  • O34-7 血疾で発見されたカテーテルアブレーション後肺静脈狭窄症の1例((症例)その他1,一般演題(口演),第37回日本呼吸器内視鏡学会学術集会)

    亀田 優美, 黒沼 幸治, 夏井坂 元基, 梅田 泰淳, 成田 欣史, 北田 順也, 大塚 満雄, 千葉 弘文, 山田 玄, 高橋 弘毅

    気管支学 : 日本気管支研究会雑誌   36 ( 0 )   2014.3

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  • 特発性肺線維症におけるピルフェニドンの治療効果予測因子としての血清SP‐D

    池田貴美之, 白鳥正典, 横尾慶紀, 梅田泰淳, 汐谷心, 錦織博貴, 黒沼幸治, 大塚満雄, 千葉弘文, 高橋弘毅

    日本呼吸器学会誌   3   126   2014.3

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  • 特発性肺線維症における血清マーカーとピルフェニドンの治療効果との関連についての検討

    池田貴美之, 白鳥正典, 横尾慶紀, 黒沼幸治, 大塚満雄, 千葉弘文, 高橋弘毅

    日本内科学会雑誌   103   167   2014.2

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  • 北海道における臨床調査個人票に基づく特発性間質性肺炎の疫学調査

    千葉弘文, 夏井坂元基, 大塚満雄, 黒沼幸治, 工藤和実, 白鳥正典, 高橋弘毅

    日本呼吸器学会誌   2   157   2013.3

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  • Two cases of the alkaline phosphatase flare phenomenon following the administration of gefitinib therapy in patients with lung adenocarcinoma with bone metastases

    Masami Kameda, Koji Kuronuma, Satsuki Miyajima, Hirofumi Chiba, Gen Yamada, Hiroki Takahashi

    Japanese Journal of Lung Cancer   53 ( 4 )   329 - 335   2013

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    DOI: 10.2482/haigan.53.329

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  • 4.再発性多発軟骨炎を疑った気管・気管支軟化症の2例(第34回 日本呼吸器内視鏡学会北海道支部会)

    成田 欣史, 梅田 泰淳, 汐谷 心, 亀田 優美, 宮島 さつき, 北村 康夫, 北田 順也, 黒沼 幸治, 大塚 満雄, 千葉 弘文, 山田 玄, 高橋 弘毅

    気管支学 : 日本気管支研究会雑誌   34 ( 6 )   642 - 642   2012.11

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    DOI: 10.18907/jjsre.34.6_642_2

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  • SP-AによるTLR2を介したブレオマイシン誘導肺障害の制御機構

    黒沼 幸治, 高橋 弘毅

    日本肺サーファクタント・界面医学会雑誌 = Journal of Japanese Medical Society for Lung Surfactant and Biological Interface   43   33 - 34   2012.10

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  • 1.エンドサイトスコピーを用いた腫瘍性病変の観察(第33回 日本呼吸器内視鏡学会北海道支部会)

    北村 康夫, 山田 裕一, 梅田 泰淳, 成田 欣史, 亀田 優美, 宮島 さつき, 北田 順也, 黒沼 幸治, 大塚 満雄, 山田 玄, 高橋 弘毅

    気管支学 : 日本気管支研究会雑誌   34 ( 1 )   85 - 85   2012.1

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    DOI: 10.18907/jjsre.34.1_85_2

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  • アフリカ産成体肺魚呼吸器の免疫組織化学的研究

    松村 豪一, 秋谷 昭治, 佐々木 美香, 武井 恒知, 千田 勝一, 佐藤 茂, 高橋 常男, 黒沼 幸治, 高橋 弘毅

    日本肺サーファクタント・界面医学会雑誌 = Journal of Japanese Medical Society for Lung Surfactant and Biological Interface   42   23 - 24   2011.10

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  • P22-4 高度な気管狭窄を認めた成人T細胞白血病リンパ腫の一例(リンパ腫,ポスター22,第34回日本呼吸器内視鏡学会学術集会)

    成田 欣史, 北田 順也, 北村 康夫, 本間 裕敏, 黒沼 幸治, 大塚 満雄, 高橋 弘毅

    気管支学 : 日本気管支研究会雑誌   33 ( 0 )   S276   2011.5

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    DOI: 10.18907/jjsre.33.Special_S276_1

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  • 肺サーファクタントリン脂質のマイコプラズマに対する生体防御機構

    黒沼 幸治, 千葉 弘文, 白鳥 正典, 高橋 弘毅, 黒木 由夫, VOELKER Dennis R

    日本肺サーファクタント・界面医学会雑誌 = Journal of Japanese Medical Society for Lung Surfactant and Biological Interface   41   2010.10

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  • Pathogenesis of Pulmonary Mycoplasma pneumoniae infection

    TANAKA H, KURONUMA K, TAKAHASHI H

    Japanese journal of mycoplasmology   36   16 - 19   2010.3

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  • Innate Immunity in Airway : Host Defense Lectins and Endotoxin Receptors

    Kuroki Yoshio, Kuronuma Koji, Kudo Kazumi, Chiba Hirofumi

    The journal of the Japan Society for Bronchology   24 ( 8 )   595 - 602   2002.12

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    The importance of innate immunity that functions as the first line defense has been well recognized since infections have been increased in patients with AIDS or maligant tumors. Innate immunity is the most fundamental host defense that distinguishes self from nonseif, protects self and eliminate nonself by recognizing pathogen associated molecular patterns (PAMPs). CD14 and Toll-like receptors (TLRs), and lectins are the pattern recognition molecules that recognize pathogen components including lipopolysaccharide (LPS) and peptidoglycan (PGN). CD 14 and TLRs functions as endotoxin receptors. They are essential for the signal transduction induced by endotoxin and are called as pattern recognition receptors. Mannose-binding proteins (MBP) , pulmonary surfactant proteins A and D (SP-A and SP-D) and conglutinin belong to the collectin subgroup of C-type lectin superfamily that possess a collagen-ilke domain. Collectins directly bind to microorganism including gram-negative bacteria, mycobacterium tuberculosis, Pneummocystis carinii, influenza virus and function against pathogens as the first line defense. Since the respiratory system is always open to the external circumstances and is exposed to high risk of pathogens&#039; invasion, the innate immune functions mediated by lung colectins SP-A and SP-D are crucially important.

    DOI: 10.18907/jjsre.24.8_595

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  • 間質性肺炎・急性肺傷害における肺胞II型上皮細胞の役割(びまん性肺疾患に対する分子生物学的アプローチ : 間質性肺炎から肺気腫まで)(第24回日本気管支学会総会)

    白鳥 正典, 相坂 治彦, 大塚 満雄, 今井 良成, 今 勇人, 藤嶋 卓哉, 黒沼 幸治, 原田 一暁, 明田 晶子, 工藤 和実, 高橋 弘毅, 阿部 庄作

    気管支学 : 日本気管支研究会雑誌   23 ( 8 )   721 - 725   2001.12

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    間質性肺炎・急性肺傷害では, 肺胞中隔への炎症細胞浸潤と線維芽細胞の増加による末梢肺組織の線維化が認められる。肺胞I型上皮細胞(type I cell)が傷害されると肺胞II型上皮細胞(type II cell)が肥大・増生し、type I cellへと分化, 肺胞壁を被覆し修復する方向へ働く。type II cellから合成・分泌される肺サーファクタント蛋白質(Surfactant Protein;SP)のうち, 疎水性であるSP-B, SP-Cは, 末梢肺組織の線維化の契機の1つと考えられている肺胞の虚脱を防ぐ機能を担う。我々は間質性肺炎における血清マーカーとしてのSP-A, SP-Dの有用性を報告してきたが, 放射線起因急性肺傷害動物モデルを用いて生体内での各SPの推移を検討した結果, 放射線照射後にサーファクタント産生の増加を認めたが, SPのmRNA発現においては, SP-A mRNAに対するSP-B, SP-C mRNA発現の相対的低下を認めた。このことから, 急性肺傷害における肺サーファクタント組成の変化は肺胞表面張力上昇を惹起させ, 肺胞虚脱による呼吸状態の悪化や末梢肺組織の線維化の一因となることが推測された。最近ではARDSに対するサーファクタント補充療法等が報告され, 今後, type II cellからみた間質性肺炎・急性肺傷害の病態に関する新たな検討が期待される。

    DOI: 10.18907/jjsre.23.8_721

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  • 8.嚢胞様陰影を呈した細気管支肺胞上皮癌の1症例(第26回 日本肺癌学会北海道支部会)

    黒沼 幸治, 中川 晃, 表 多希子, 前田 喜晴, 佐藤 正文, 平口 悦郎

    肺癌   40 ( 7 )   797 - 797   2000.12

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    Language:Japanese   Publisher:日本肺癌学会  

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  • Small Cell Lung Cancer Associated with Anti-Hu Antibody-positive Paraneoplastic Neurologic Syndrome

    KUROMUMA Koji, NISHIYAMA Kaoru, MURAKAMI Seiji, TANAKA Nobuyuki, TAKAHASHI Mamoru, KOJIMA Hiroshi, FUJISHIMA Takuya, TANAKA Hiroshi, TAKAHASHI Hiroki, KOBA Hiroyuki, TANAKA Keiko, ABE Syosaku

    38 ( 2 )   148 - 152   2000.2

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    Language:Japanese  

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  • Mothers' Attitude Toward Pupils with Common Cold

    NISHI Motoi, MIYAKE Hirotsugu, OZASA Yasuhiro, OMOTE Takiko, KIKUCHI Yuka, KURONUMA Kouji, TAKAHASHI Syou, NAKAYAMA Ichirou, MONIWA Norihito

    The Jounral of child health   56 ( 1 )   88 - 91   1997.1

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  • An Investigation of Hospital Treatment of Pupils with Common Cold

    NISHI Motoi, MIYAKE Hirotsugu, OZASA Yasuhiro, OMOTE Takiko, KIKUCHI Yuka, KURONUMA Kouji, TAKAHASHI Syou, NAKAYAMA Ichirou, MONIWA Norihito

    The Jounral of child health   55 ( 6 )   763 - 767   1996.11

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Research Projects

  • 肺マイクロバイオームの変容と自然免疫応答異常に注目したIPF病態の解明

    Grant number:22K08234  2022.4 - 2025.3

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    千葉 弘文, 齋藤 充史, 黒沼 幸治

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    Grant amount:\4030000 ( Direct Cost: \3100000 、 Indirect Cost:\930000 )

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  • 肺マイクロバイオーム多様性喪失と肺サーファクタント蛋白質の免疫機構

    Grant number:20K08570  2020.4 - 2023.3

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    黒沼 幸治, 齋藤 充史, 千葉 弘文

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    Grant amount:\4420000 ( Direct Cost: \3400000 、 Indirect Cost:\1020000 )

    肺内に豊富に存在する肺サーファクタント蛋白質は、様々な細菌と結合し下気道の自然免疫・感染防御の役割を担っている。マイクロバイオーム解析の進歩により、肺における微生物叢のホメオスタシス変化と様々な疾患との関連に注目が集まっている。高齢化に伴い繰り返す感染や慢性的な肺損傷による肺サーファク タント蛋白質の喪失は肺内微生物叢環境に異常をもたらすと考えられる。 肺サーファクタント蛋白質のうちSP-AとSP-Dは様々な細菌、真菌の菌体成分に結合し、オプソニン効果やToll様受容体を介した炎症制御、肺胞マクロファージ の活性化などの働きがあり、炎症を制御する。申請者らはこれまで、SP-AとSP-Dが肺炎球菌感染に対し、抗炎症作用と抗菌活性を有することを報告している。申請者らが報告した特発性肺線維 症における肺マイクロバイオームのDysbiosisは疾患予後とも密接に関連している。
    マウスのBAL液のマイクロバイオームを検討したところ、25週齢のSP-A欠損マウスで多様性喪失(Dysbiosis)がみられた。
    抗線維化薬であるピルフェニドンは光線過敏症や悪心を誘発し、ペラグラ様の病態を呈する。マウスに低ナイアシン食を与えてペラグラモデルを作成し、マウスのマイクロバイオームを解析したところ腸内細菌叢に変化がみられた。低ナイアシン食でBacteroidaceae、Streptococcaceaeの減少とRuminococcaceaeの増加を認めた。代謝産物の解析では尿中MNA、NMO、2-Py、4-Pyの減少を認めた。皮膚のアラキドン酸の分析でもHETEやEETの上昇を認め酸化脂肪酸の影響が示唆された。ピルフェニドンモデルマウスで今後マイクロバイオーム解析を行い、肺サーファクタント組成変化の解析と共に副作用の少ない新たな治療法を探索する。

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  • Pulmonary fibrosis treatment with pulmonary surfactant as a TLR regulatory molecule and HSP47 inhibition

    Grant number:17K09662  2017.4 - 2020.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Takahashi Hiroki

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    Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )

    Objective: To develop a new therapeutic method for idiopathic pulmonary fibrosis under the hypothesis that surfactant protein (SP) -A supplementation and HSP47 siRNA suppress lung inflammation and fibrosis. Method: BLM was intratracheally administered to SP-A (-/-) and wild-type mice to prepare a lung injury / fibrosis model, and siRNA HSP47 was intravenously administered. Results: Inflammation and fibrosis were enhanced in SP-A deficient mice compared to wild type. In addition, siRNA HSP47 reduced lung injury and suppressed BLM-induced airway inflammation. Conclusion: SP-A supplementation and siRNA HSP47 suppressed the formation of lung injury / fibrotic lesions, and it was shown that the combination of both could be one of the new treatments for pulmonary fibrosis.

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  • Pulmonary collectin regulates the severity of Streptococcus pneumoniae infection

    Grant number:15K09181  2015.4 - 2018.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Kuronuma Koji

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    Grant amount:\4810000 ( Direct Cost: \3700000 、 Indirect Cost:\1110000 )

    Pneumococcal infections tend to be severe, especially invasive pneumococcal infections also have a high mortality rate. Differences in the severity of pneumococcal infection caused by capsular serotypes have been reported by epidemiological studies. Our surveillance in Hokkaido has also revealed the serotype replacement of invasive pneumococcal infections which is related to the spread of pneumococcal vaccines. We confirmed that lung collectin specifically binds to pneumococcal components and activates macrophages and increases antibiotic activity by increasing the receptors necessary for phagocytosis of bacteria. It became clear that the relation with the regulatory factors such as vaccine effect, host immune status and pulmonary collectin is important as a pathological condition of pneumococcus.

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  • Suppression of pulmonary injury and fibrosis by applying new action of pulmonary surfactant protein

    Grant number:26461164  2014.4 - 2017.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    TAKAHASHI HIROKI, HASEGAWA YOSHIHIRO, SAITO ATSUSHI

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    Grant amount:\4940000 ( Direct Cost: \3800000 、 Indirect Cost:\1140000 )

    This study was performed to clarify the importance of pulmonary surfactant protein (SP-A) as an inhibitor of lung injury / fibrosis and allergic airway inflammation and its mechanism, and to show the results that will be a bridge to the therapeutic strategy. In SP-A knockout mice, intrapulmonary inflammation and fibrosis were significantly enhanced under bleomycin stimulation compared with wild type mice. When SP-A was further administered from the respiratory tract, the inflammation was suppressed. Furthermore, in vitro experiments revealed that the inhibitory effect of SP-A against inflammation was based on the inhibition of binding of bleomycin and soluble Toll-like receptor (sTLR2). In addition, the effect of suppressing allergic reaction in respiratory tracts was also confirmed.

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  • Therapeutic application of lung collectin in acute lung injury

    Grant number:23591154  2011 - 2013

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    TAKAHASHI HIROKI, KUROKI Yoshio, SHIRATORI Masanori, CHIBA Hirofumi, KUDO Kazumi, KURONUMA Koji

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    Grant amount:\5330000 ( Direct Cost: \4100000 、 Indirect Cost:\1230000 )

    The purpose of this project were to clarify the mechanism of regulation by which Toll-like receptors (TLRs) act against drug-induced acute lung injury (ALI), to evaluate the protective effect by lung a collectin, SP-A and to establish the molecular basis for the clinical application of it. Bleomycin (BLM)-administered SP-A(-/-) mice had significantly higher mortality rate and more increase of inflammatory cytokines in the lungs than wild types. SP-A inhibited BLM-induced inflammatory cytokines from rat alveolar macrophages. sTLR2 gene-transfected HEK293 cells showed up-regulation of NF-kB. BML bound directly with sTLR2 and its binding was blocked by SP-A. These results indicate that BLM-induced signal depends on TLR2 and lung collectin, SP-A has protective function against ALI via TLR2 dependent signal pathway.

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  • Pulmonary surfactant protein A modulates non-infectious lung injury.

    Grant number:23790915  2011 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Young Scientists (B)

    KURONUMA Koji

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    Grant amount:\4160000 ( Direct Cost: \3200000 、 Indirect Cost:\960000 )

    Since non-infectious pulmonary disorders can progress to a fatal clinical condition due to excessive production of inflammatory cytokine, the purpose of this study is to understand the lung protective role of pulmonary surfactant protein A (SP-A). SP-A deficient mice demonstrated the low survival rate, the increase of cell count in bronchoalveolar lavage fluid, and the production of proinflammatory cytokine following the transairway administration of bleomycin. SP-A might control innate immune systemby binding to bleomycin strongly and directly, and inhibiting bleomycin from binding to toll-like receptor.

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  • Pulmonary collectin regulates drug-induced lung injury.

    Grant number:20590930  2008 - 2010

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    TAKAHASHI Hiroki, KUROKI Yoshio, SHIRATORI Masanori, CHIBA Hirofumi, KUDO Kazumi, KURONUMA Koji

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    Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )

    The purposes of this project were to investigate the mechanisms of regulation by which lung collectins, surfactant proteins A and D (SP-A and SP-D), and Toll-like receptor (TLR) function against inflammation on the development of drug induced lung injury, and to establish the molecular basis for the clinical application of these proteins. First, the mouse model of lung injury was established using drugs, and SP-A^<-/-> mice had significantly higher mortality rates than WT mice. SP-A inhibited drug-induced inflammatory cytokine production from rat alveolar macrophages. SP-A blocked sTLR2-bleomycin binding in the dose dependent manner. These results suggest that bleomycin signals are transmitted through TLR2 dependent pathway and regulated by the molecular interaction of SP-A.

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