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• 2020年04月

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  継続中

  札幌医科大学   医学部 附属フロンティア医学研究所   准教授

• 2012年08月

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  2020年03月

  札幌医科大学   医学部 附属フロンティア医学研究所   講師

• 2012年01月

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  2012年07月

  札幌医科大学 医学部   医学部 附属フロンティア医学研究所   助教

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• ライフサイエンス   脳神経外科学  

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• 札幌医科大学   医学部 附属フロンティア医学研究所   准教授  

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• Repeated intravenous infusion of mesenchymal stem cells enhances recovery of motor function in a rat model with chronic spinal cord injury.

  Kota Kurihara, Masanori Sasaki, Hiroshi Nagahama, Hisashi Obara, Ryunosuke Fukushi, Ryosuke Hirota, Mitsunori Yoshimoto, Atsushi Teramoto, Jeffery D Kocsis, Toshihiko Yamashita, Osamu Honmou

  Brain research   1817   148484 - 148484  2023年10月  [国際誌]

  　概要を見る

  Spinal cord injury (SCI) can cause paralysis with a high disease burden with limited treatment options. A single intravenous infusion of mesenchymal stem cells (MSCs) improves motor function in rat SCI models, possibly through the induction of axonal sprouting and remyelination. Repeated infusions (thrice at weekly intervals) of MSCs were administered to rats with chronic SCI to determine if multiple-dosing regimens enhance motor improvement. Chronic SCI rats were randomized and infused with vehicle (vehicle), single MSC injection at week 6 (MSC-1) or repeatedly injections of MSCs at 6, 7, and 8 weeks (MSC-3) after SCI induction. In addition, a single high dose of MSCs (HD-MSC) equivalent to thrice the single dose was infused at week 6. Locomotor function, light and electron microscopy, immunohistochemistry and ex vivo diffusion tensor imaging were performed. Repeated infusion of MSCs (MSC-3) provided the greatest functional recovery compared to single and single high-dose infusions. The density of remyelinated axons in the injured spinal cord was the greatest in the MSC-3 group, followed by the MSC-1, HD-MSC and vehicle groups. Increased sprouting of the corticospinal tract and serotonergic axon density was the greatest in the MSC-3 group, followed by MSC-1, HD-MSC, and vehicle groups. Repeated infusion of MSCs over three weeks resulted in greater functional improvement than single administration of MSCs, even when the number of infused cells was tripled. MSC-treated rats showed axonal sprouting and remyelination in the chronic phase of SCI.

  DOI PubMed

• Therapeutic efficacy of intravenous infusion of mesenchymal stem cells in rat perinatal brain injury.

  Kojiro Terada, Masanori Sasaki, Hiroshi Nagahama, Yuko Kataoka-Sasaki, Shinichi Oka, Ryo Ukai, Takahiro Yokoyama, Yusuke Iizuka, Takuro Sakai, Shinobu Fukumura, Takeshi Tsugawa, Jeffery D Kocsis, Osamu Honmou

  Pediatric research    2023年07月  [国際誌]

  　概要を見る

  BACKGROUND: Perinatal brain injury is multifactorial and primarily associated with brain prematurity, inflammation, and hypoxia-ischemia. Although recent advances in perinatal medicine have improved the survival rates of preterm infants, neurodevelopmental disorders remain a significant complication. We tested whether the intravenous infusion of mesenchymal stem cells (MSCs) had therapeutic efficacy against perinatal brain injury in rats. METHODS: Pregnant rats at embryonic day (E) 18 received lipopolysaccharide and the pups were born at E21. On postnatal day (PND) 7, the left common carotid artery of each pup was ligated, and they were exposed to 8% oxygen for 2 h. They were randomized on PND10, and MSCs or vehicle were intravenously infused. We performed behavioral assessments, measured brain volume using MRI, and performed histological analyses on PND49. RESULTS: Infused MSCs showed functional improvements in our model. In vivo MRI revealed that MSC infusion increased non-ischemic brain volume compared to the vehicle group. Histological analyses showed that cortical thickness, the number of NeuN+ and GAD67+ cells, and synaptophysin density in the non-ischemic hemisphere in the MSC group were greater than the vehicle group, but less than the control group. CONCLUSIONS: Infused MSCs improve sensorimotor and cognitive functions in perinatal brain injury and enhance neuronal growth. IMPACT: Intravenous infusion of MSCs improved neurological function in rats with perinatal brain injury, including motor, sensorimotor, cognitive, spatial, and learning memory. Infused MSCs increased residual (non-ischemic) tissue volume, number of neuronal cells, GABAergic cells, and cortical synapses in the contralesional (right) hemisphere. Intravenous administration of MSC might be suitable for the treatment of perinatal brain injury.

  DOI PubMed

• A practical protocol for high-spatial-resolution magnetic resonance angiography for cerebral arteries in rats.

  Hiroshi Nagahama, Masanori Sasaki, Katsuya Komatsu, Kaori Sato, Yoshimi Katagiri, Masaki Kamagata, Yuko Kataoka-Sasaki, Shinichi Oka, Ryo Ukai, Takahiro Yokoyama, Kojiro Terada, Masato Kobayashi, Jeffery D Kocsis, Osamu Honmou

  Journal of neuroscience methods   386   109784 - 109784  2023年01月  [国際誌]

  　概要を見る

  BACKGROUND: Magnetic resonance angiography (MRA) is an important tool in rat models of cerebrovascular disease. Although MRA has long been used in rodents, the image quality is typically not as high as that observed in clinical practice. Moreover, studies on MRA image quality in rats are limited. This study aimed to develop a practical high-spatial-resolution MRA protocol for imaging cerebral arteries in rats. NEW METHOD: We used the "half position method" regarding coil placement and modified the imaging parameters and image reconstruction method. We applied this new imaging method to measure maturation-related signal changes on rat MRAs. RESULTS: The new practical high-spatial-resolution MRA imaging protocol obtained a signal intensity up to 3.5 times that obtained using a basic coil system, simply by modifying the coil placement method. This method allowed the detection of a gradual decrease in the signal in cerebral vessels with maturation. COMPARISON WITH EXISTING METHODS: A high-spatial-resolution MRA for rats was obtained with an imaging time of approximately 100 min. Comparable resolution and image quality were obtained using the new protocol with an imaging time of 30 min CONCLUSIONS: The new practical high-spatial-resolution MRA protocol can be implemented simply and successfully to achieve high image quality with an imaging time of approximately 30 min. This protocol will benefit researchers performing MRA imaging in cerebral artery studies in rats.

  DOI PubMed

• Intravenous infusion of bone marrow-derived mesenchymal stem cells improves tissue perfusion in a rat hindlimb ischemia model.

  Shusaku Maeda, Takuji Kawamura, Masanori Sasaki, Kazuo Shimamura, Takashi Shibuya, Akima Harada, Osamu Honmou, Yoshiki Sawa, Shigeru Miyagawa

  Scientific reports   12 ( 1 ) 16986 - 16986  2022年10月  [国際誌]

  　概要を見る

  Intravenous infusion of stem cells is a minimally invasive cellular delivery method, though a few have been reported in a critical limb-threatening ischemia (CLTI) animal model or patients. In the present study, we hypothesized that intravenous infusion of bone-marrow derived mesenchymal stem cells (MSCs) improves tissue perfusion in a rat hindlimb ischemia model. Hindlimb ischemia was generated in Sprague-Dawley rats by femoral artery removal, then seven days after ischemic induction intravenous infusion of 1 × 106 MSCs (cell group) or vehicle (control group) was performed. As compared with the control, tissue perfusion was significantly increased in the cell group. Histological findings showed that capillary density was significantly increased in the cell group, with infused green fluorescent protein (GFP)-MSCs distributed in the ischemic limb. Furthermore, gene expression of vascular endothelial growth factor (VEGF) was significantly increased in ischemic hindlimb muscle tissues of rats treated with MSC infusion. In conclusion, intravenous infusion of bone-marrow derived MSCs improved tissue perfusion in ischemic hindlimbs through angiogenesis, suggesting that intravenous infusion of MSCs was a promising cell delivery method for treatment of CLTI.

  DOI PubMed

• Enhanced Network in Corticospinal Tracts after Infused Mesenchymal Stem Cells in Spinal Cord Injury

  Ryosuke Hirota, Masanori Sasaki, Yuko Kataoka-Sasaki, Tsutomu Oshigiri, Kota Kurihara, Ryunosuke Fukushi, Shinichi Oka, Ryo Ukai, Mitsunori Yoshimoto, Jeffery D. Kocsis, Toshihiko Yamashita, Osamu Honmou

  Journal of Neurotrauma ( Mary Ann Liebert Inc )  39 ( 23-24 ) 1665 - 1677  2022年07月  [国際誌]

  　概要を見る

  Although limited spontaneous recovery occurs after spinal cord injury (SCI), current knowledge reveals that multiple forms of axon growth in spared axons can lead to circuit reorganization and a detour or relay pathways. This hypothesis has been derived mainly from studies of the corticospinal tract (CST), which is the primary descending motor pathway in mammals. The major CST is the dorsal CST (dCST), being the major projection from cortex to spinal cord. Two other components often called "minor" pathways are the ventral and the dorsal lateral CSTs, which may play an important role in spontaneous recovery. Intravenous infusion of mesenchymal stem cells (MSCs) provides functional improvement after SCI with an enhancement of axonal sprouting of CSTs. Detailed morphological changes of CST pathways, however, have not been fully elucidated. The primary objective was to evaluate detailed changes in descending CST projections in SCI after MSC infusion. The MSCs were infused intravenously one day after SCI. A combination of adeno-associated viral vector (AAV), which is an anterograde and non-transsynaptic axonal tracer, was injected 14 days after SCI induction. The AAV with advanced tissue clearing techniques were used to visualize the distribution pattern and high-resolution features of the individual axons coursing from above to below the lesion. The results demonstrated increased observable axonal connections between the dCST and axons in the lateral funiculus, both rostral and caudal to the lesion core, and an increase in observable axons in the dCST below the lesion. This increased axonal network could contribute to functional recovery by providing greater input to the spinal cord below the lesion.

  DOI PubMed

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• 虚血性脊髄障害モデルに対する間葉系幹細胞静脈投与の治療効果の検討—Intravenous delivery of mesenchymal stem cells protects both white and gray matter in spinal cord ischemia

  安田 尚美, 佐々木 祐典, 佐々木 優子, 長濱 宏, Jeffry D. Kocsis, 川原田 修義, 本望 修

  北海道外科雑誌 = The Hokkaido journal of surgery / 北海道外科雑誌編集委員会 編 ( [札幌] : 北海道外科学会 )  66 ( 1 ) 50 - 52  2021年06月

  CiNii

• 脳小血管病と急性再開通治療3ヵ月後の臨床転帰との関係

  中崎 公仁, 野中 雅, 野村 達史, 恩田 敏之, 米増 保之, 高橋 明, 橋本 祐治, 本田 修, 佐々木 祐典, 大坊 雅彦, 本望 修

  脳血管内治療 ( (NPO)日本脳神経血管内治療学会 )  2 ( Suppl. ) S170 - S170  2017年11月

• T1 Black Blood imagingによる椎骨動脈解離の経時的変化(Temporal Change of Signal Intensity of Intramural Hematoma in Patients with Acute Vertebral Artery Dissection Using T1-Weighted Black Blood Imaging)

  本田 修, 中崎 公仁, 佐々木 祐典, 野中 雅, 高橋 明, 橋本 祐治, 米増 保之, 野村 達史, 恩田 敏之, 大坊 雅彦

  CI研究 ( 日本脳神経CI学会 )  39 ( 2 ) 71 - 77  2017年11月

• 脳小血管病と緊急血栓回収療法の臨床転帰との関係

  中崎公仁, 野中雅, 野村達史, 恩田敏之, 野中雅, 野村達史, 恩田敏之, 米増保之, 高橋明, 橋本祐治, 本田修, 佐々木祐典, 大坊雅彦, 本望修

  脳循環代謝(Web) ( 日本脳循環代謝学会 )  29 ( 1 ) 159 - 159  2017年11月

  J-GLOBAL

• MRI diffusion tensor imaging(DTI)法を用いた脊髄損傷動物モデルに対する骨髄間葉系幹細胞移植における治療効果判定の検討

  押切 勉, 佐々木 祐典, 森田 智慶, 長濱 宏史, 本望 修, 山下 敏彦

  日本整形外科学会雑誌 ( (公社)日本整形外科学会 )  91 ( 8 ) S1502 - S1502  2017年08月

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共同研究・競争的資金等の研究課題 【 表示 ／ 非表示 】

共同研究・競争的資金等の研究課題 【 表示 ／ 非表示 】

• 骨髄間葉系幹細胞移植による慢性腎臓病および連関する脳血管障害の同時進行抑制

  基盤研究(C)

  研究期間:

  2023年04月

  -

  2026年03月

   

  長岡 由修, 飯塚 裕典, 寺田 光次郎, 津川 毅, 佐々木 祐典, 本望 修

• 新生児低酸素性虚血性脳症に対する次世代型エクソソームを用いた新規治療法の開発

  基盤研究(C)

  研究期間:

  2023年04月

  -

  2026年03月

   

  寺田 光次郎, 津川 毅, 飯塚 裕典, 佐々木 祐典, 長濱 宏史, 坂井 拓朗, 本望 修

• 骨髄間葉系幹細胞を用いた難治性てんかんの新規治療法開発とメカニズム解明

  基盤研究(C)

  研究期間:

  2022年04月

  -

  2025年03月

   

  福村 忍, 津川 毅, 佐々木 祐典, 長濱 宏史, 本望 修

• 脊損に対する骨髄幹細胞治療で介在ニューロンの関与で再構築された神経回路の解析

  基盤研究(C)

  研究期間:

  2022年04月

  -

  2025年03月

   

  小原 尚, 福士 龍之介, 廣田 亮介, 佐々木 祐典, 栗原 康太, 山下 敏彦, 佐々木 優子, 本望 修

• 脳梗塞と脊髄損傷に対する中枢神経系全域の可塑性賦活化による治療戦略の検討

  基盤研究(B)

  研究期間:

  2022年04月

  -

  2025年03月

   

  本望 修, 佐々木 祐典, 鵜飼 亮, 岡 真一, 佐々木 優子

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