TUGAWA Takesi

写真a

Affiliation

School of Medicine, Department of Pediatrics

Job title

Professor

Profile

2007.3月-2010.3月　アメリカ国立衛生研究所（NIH/NIAID）

2012.4月　札幌医科大学　小児科

2016.4月　同　講師

2021.9月　同　教授

 
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Education 【 display / non-display 】

  • 　
    -
    2005

    Sapporo Medical University   Faculty of Medicine  

  • 　
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    1998

    Sapporo Medical University   医学部   医学科  

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    卒業

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Degree 【 display / non-display 】

  • 札幌医科大学   医学博士

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Professional Memberships 【 display / non-display 】

  • 2022
    -
    Now

    日本ワクチン学会

  • 2021
    -
    Now

    日本感染症学会

  • 2021
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    Now

    日本化学療法学会

  • 2011
    -
    Now

    日本小児保健協会

  • 2004
    -
    Now

    日本ウイルス学会

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Research Areas 【 display / non-display 】

  • Life sciences   Fetal medicine/Pediatrics  

  • Life sciences   Virology   ロタウイルス、ノロウイルス、サポウイルス

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Affiliation 【 display / non-display 】

  • Sapporo Medical University   Graduate School of Medicine   講師  

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Research Interests 【 display / non-display 】

  • ウイルス学

  • 小児科学

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Papers 【 display / non-display 】

  • A significant outbreak of respiratory human adenovirus infections among children aged 3-6 years in Hokkaido, Japan, in 2023.

    Yuya Fukuda, Atsuo Togashi, Satoshi Hirakawa, Masaki Yamamoto, Shinobu Fukumura, Tomohiro Nawa, Nana Kushima, Satoshi Nakamura, Jun Kunizaki, Kouhei Nishino, Ryoma Kimura, Toshitaka Kizawa, Dai Yamamoto, Ryoh Takeuchi, Yuta Sasaoka, Masayoshi Kikuchi, Takuro Ito, Kazushige Nagai, Hirofumi Asakura, Sayaka Nishimura, Masaki Yoshida, Kousuke Tsuchida, Takeshi Tsugawa

    Journal of medical virology   96 ( 7 ) e29780  2024.07  [Refereed]  [International journal]

    Authorship：   Last author

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    Human adenovirus (HAdV) infections present diverse clinical manifestations upon infecting individuals, with respiratory infections predominating in children. We surveyed pediatric hospitalizations due to respiratory HAdV infections across 18 hospitals in Hokkaido Prefecture, Japan, from July 2019 to March 2024, recording 473 admissions. While hospitalizations remained below five cases per week from July 2019 to September 2023, a notable surge occurred in late October 2023, with weekly admissions peaking at 15-20 cases from November to December. There were dramatic shifts in the age distribution of hospitalized patients: during 2019-2021, 1-year-old infants and children aged 3-6 years represented 51.4%-54.8% and 4.1%-13.3%, respectively; however, in 2023-2024, while 1-year-old infants represented 19.0%-20.1%, the proportion of children aged 3-6 years increased to 46.2%-50.0%. Understanding the emergence of significant outbreaks of respiratory HAdV infections and the substantial changes in the age distribution of hospitalized cases necessitates further investigation into the circulating types of HAdV in Hokkaido Prefecture and changes in children's neutralizing antibody titers against HAdV.

    DOI PubMed

  • Whole-genome analysis of human group A rotaviruses in 1980s Japan and evolutionary assessment of global Wa-like strains across half a century.

    Yuya Fukuda, Kenji Kondo, Shuji Nakata, Yasuyuki Morita, Noriaki Adachi, Keiko Kogawa, Susumu Ukae, Yoshimasa Kudou, Shuhei Adachi, Masaki Yamamoto, Shinobu Fukumura, Takeshi Tsugawa

    The Journal of general virology   105 ( 6 )  2024.06  [Refereed]  [International journal]

    Authorship：   Last author

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    Historically, the Wa-like strains of human group A rotavirus (RVA) have been major causes of gastroenteritis. However, since the 2010s, the circulation of non-Wa-like strains has been increasingly reported, indicating a shift in the molecular epidemiology of RVA. Although understanding RVA evolution requires the analysis of both current and historical strains, comprehensive pre-1980's sequencing data are scarce globally. We determined the whole-genome sequences of representative strains from six RVA gastroenteritis outbreaks observed at an infant home in Sapporo, Japan, between 1981 and 1989. These outbreaks were mainly caused by G1 or G3 Wa-like strains, resembling strains from the United States in the 1970s-1980s and from Malawi in the 1990s. Phylogenetic analysis of these infant home strains, together with Wa-like strains collected worldwide from the 1970s to 2020, revealed a notable trend: pre-2010 strains diverged into multiple lineages in many genomic segments, whereas post-2010 strains tended to converge into a single lineage. However, Bayesian skyline plot indicated near-constant effective population sizes from the 1970s to 2020, and selection pressure analysis identified positive selection only at amino acid 75 of NSP2. These results suggest that evidence supporting the influence of rotavirus vaccines, introduced globally since 2006, on Wa-like RVA molecular evolution is lacking at present, and phylogenetic analysis may simply reflect natural fluctuations in RVA molecular evolution. Evaluating the long-term impact of RV vaccines on the molecular evolution of RVA requires sustained surveillance.

    DOI PubMed

  • Human transmission and outbreaks of feline-like G6 rotavirus revealed with whole-genome analysis of G6P[9] feline rotavirus.

    Yuya Fukuda, Hajime Kusuhara, Reiko Takai-Todaka, Kei Haga, Kazuhiko Katayama, Takeshi Tsugawa

    Journal of medical virology   96 ( 4 ) e29565  2024.04  [Refereed]  [International journal]

    Authorship：   Last author

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    Group A rotaviruses (RVAs) are generally highly species-specific; however, some strains infect across species. Feline RVAs sporadically infect humans, causing gastroenteritis. In 2012 and 2013, rectal swab samples were collected from 61 asymptomatic shelter cats at a public health center in Mie Prefecture, Japan, to investigate the presence of RVA and any association with human infections. The analysis identified G6P[9] strains in three cats and G3P[9] strains in two cats, although no feline RVA sequence data were available for the former. A whole-genome analysis of these G6P[9] strains identified the genotype constellation G6-P[9]-I2-R2-C2-M2-A3-N2-T3-E3-H3. The nucleotide identity among these G6P[9] strains exceeded 99.5% across all 11 gene segments, indicating the circulation of this G6P[9] strain among cats. Notably, strain RVA/Human-wt/JPN/KF17/2010/G6P[9], previously detected in a 3-year-old child with gastroenteritis, shares high nucleotide identity (>98%) with Mie20120017f, the representative G6P[9] strain in this study, across all 11 gene segments, confirming feline RVA infection and symptomatic presentation in this child. The VP7 gene of strain Mie20120017f also shares high nucleotide identity with other sporadically reported G6 RVA strains in humans. This suggests that feline-origin G6 strains as the probable source of these sporadic G6 RVA strains causing gastroenteritis in humans globally. Moreover, a feline-like human G6P[8] strain circulating in Brazil in 2022 was identified, emphasizing the importance of ongoing surveillance to monitor potential global human outbreaks of RVA.

    DOI PubMed

  • Molecular evolutionary analysis of novel NSP4 mono-reassortant G1P[8]-E2 rotavirus strains that caused a discontinuous epidemic in Japan in 2015 and 2018.

    Yoshiki Fujii, Takeshi Tsugawa, Yuya Fukuda, Shuhei Adachi, Saho Honjo, Yusuke Akane, Kenji Kondo, Yoshiyuki Sakai, Toju Tanaka, Toshiya Sato, Yoshihito Higasidate, Noriaki Kubo, Toshihiko Mori, Shinsuke Kato, Ryo Hamada, Masayoshi Kikuchi, Yasuo Tahara, Kazushige Nagai, Toshio Ohara, Masaki Yoshida, Shuji Nakata, Atsuko Noguchi, Wakako Kikuchi, Hiromichi Hamada, Shoko Tokutake-Hirose, Makoto Fujimori, Masamichi Muramatsu

    Frontiers in microbiology   15   1430557 - 1430557  2024  [Refereed]  [International journal]

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    In the 2010s, several unusual rotavirus strains emerged, causing epidemics worldwide. This study reports a comprehensive molecular epidemiological study of rotaviruses in Japan based on full-genome analysis. From 2014 to 2019, a total of 489 rotavirus-positive stool specimens were identified, and the associated viral genomes were analyzed by next-generation sequencing. The genotype constellations of those strains were classified into nine patterns (G1P[8] (Wa), G1P[8]-E2, G1P[8] (DS-1), G2P[4] (DS-1), G3P[8] (Wa), G3P[8] (DS-1), G8P[8] (DS-1), G9P[8] (Wa), and G9P[8]-E2). The major prevalent genotype differed by year, comprising G8P[8] (DS-1) (37% of that year's isolates) in 2014, G1P[8] (DS-1) (65%) in 2015, G9P[8] (Wa) (72%) in 2016, G3P[8] (DS-1) (66%) in 2017, G1P[8]-E2 (53%) in 2018, and G9P[8] (Wa) (26%) in 2019. The G1P[8]-E2 strains (G1-P[8]-I1-R1-C1-M1-A1-N1-T1-E2-H1) isolated from a total of 42 specimens in discontinuous years (2015 and 2018), which were the newly-emerged NSP4 mono-reassortant strains. Based on the results of the Bayesian evolutionary analyses, G1P[8]-E2 and G9P[8]-E2 were hypothesized to have been generated from distinct independent inter-genogroup reassortment events. The G1 strains detected in this study were classified into multiple clusters, depending on the year of detection. A comparison of the predicted amino acid sequences of the VP7 epitopes revealed that the G1 strains detected in different years encoded VP7 epitopes harboring distinct mutations. These mutations may be responsible for immune escape and annual changes in the prevalent strains.

    DOI PubMed

  • Resurgence of human metapneumovirus infection and influenza after three seasons of inactivity in the post-COVID-19 era in Hokkaido, Japan, 2022-2023.

    Yuya Fukuda, Atsuo Togashi, Satoshi Hirakawa, Masaki Yamamoto, Shinobu Fukumura, Tomohiro Nawa, Saho Honjo, Jun Kunizaki, Kouhei Nishino, Toju Tanaka, Toshitaka Kizawa, Dai Yamamoto, Ryoh Takeuchi, Yuta Sasaoka, Masayoshi Kikuchi, Takuro Ito, Kazushige Nagai, Hirofumi Asakura, Katsumasa Kudou, Masaki Yoshida, Takeshi Nishida, Takeshi Tsugawa

    Journal of medical virology   95 ( 12 ) e29299  2023.12  [Refereed]  [International journal]

    Authorship：   Last author

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    Following the coronavirus disease 2019 (COVID-19) outbreak in February 2020, incidences of various infectious diseases decreased notably in Hokkaido Prefecture, Japan. However, Japan began gradually easing COVID-19 infection control measures in 2022. Here, we conducted a survey of children hospitalized with human metapneumovirus (hMPV), influenza A and B, and respiratory syncytial virus infections in 18 hospitals across Hokkaido Prefecture, Japan, spanning from July 2019 to June 2023. From March 2020 to June 2022 (28 months), only 13 patients were hospitalized with hMPV, and two patients had influenza A. However, in October to November 2022, there was a re-emergence of hMPV infections, with a maximum of 27 hospitalizations per week. From July 2022 to June 2023 (12 months), the number of hMPV-related hospitalizations dramatically increased to 317 patients, with the majority aged 3-6 years (38.2%, [121/317]). Influenza A also showed an increase from December 2022, with a peak of 13 hospitalizations per week in March 2023, considerably fewer than the pre-COVID-19 outbreak in December 2019, when rates reached 45 hospitalizations per week. These findings suggest the possibility of observing more resurgences in infectious diseases in Japan after 2023 if infection control measures continue to be relaxed. Caution is needed in managing potential outbreaks.

    DOI PubMed

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Misc 【 display / non-display 】

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Research Projects 【 display / non-display 】

  • Intravenous infusion of exosome for the treatment of neonatal hypoxic ischemic encephalopathy

    Grant-in-Aid for Scientific Research (C)

    Project Year :

    2023.04
    -
    2026.03
     

    寺田 光次郎, 津川 毅, 飯塚 裕典, 佐々木 祐典, 長濱 宏史, 坂井 拓朗, 本望 修

  • Bone marrow mesenchymal stem cell transplantation prevents concurrent progression of chronic kidney disease and associated cerebrovascular disease

    Grant-in-Aid for Scientific Research (C)

    Project Year :

    2023.04
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    2026.03
     

    長岡 由修, 飯塚 裕典, 寺田 光次郎, 津川 毅, 佐々木 祐典, 本望 修

  • 下痢症ウイルスの分子疫学を基盤にした流行制御にかかる研究「ウイルス性胃腸炎の臨床疫学と分子生物学的解析」

    Project Year :

    2023.04
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    2026.03
     

    Authorship： Coinvestigator(s)

  • Development of novel treatments and elucidation of mechanisms for intractable epilepsy using bone marrow mesenchymal stem cells

    Grant-in-Aid for Scientific Research (C)

    Project Year :

    2022.04
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    2025.03
     

    福村 忍, 津川 毅, 佐々木 祐典, 長濱 宏史, 本望 修

  • ロタワクチン定期接種化：真の安全性・有効性・選択圧の評価モデル構築と流行株の変化

    基盤研究(C)

    Project Year :

    2021.04
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    2024.03
     

    津川 毅, 本庄 紗帆, 赤根 祐介

    　View Summary

    ロタウイルスワクチンの重症予防効果は約90%で、わが国には2011年に導入され、2020年10月の定期接種化に伴い、サーベイランスの社会的な重要性は更に増大した。ロタウイルスワクチンは有効性、安全性、選択圧の評価などが必須であるが、我々の研究により、現状の国内の胃腸炎サーベイランスでは正確な評価が困難である事が判明した。 本研究では、北海道の22研究協力施設（小児・成人）と自治体における①ロタウイルス胃腸炎の入院数・臨床像、②ロタウイルス遺伝子型、③初回接種週数・接種率、④腸重積症の入院数・臨床像・原因ウイルスの解析により、１）ロタウイルスワクチンの有効性の評価（導入前後・定期接種化前後の入院数や合併症の推移）、２）安全性の担保（初回接種週数と腸重積症の推移）、３）選択圧の検討（流行株の遺伝子解析）、４）迅速診断の精度を評価し、今後のサーベイランスのあり方、特にワクチン評価法の提案とロタウイルスの生活環の解明に迫る。 本年度の解析では、①ロタウイルス胃腸炎の入院数は、2020年が11例、2021年が0例であり、新型コロナウイルス感染症による学校閉鎖（2020年3月-5月）後に著明に減少していた。②ロタウイルス遺伝子型（入院）は、2019年は49株で、G9P[8]が23株、G1P[8]が10株、G8P[8]が9株、G2P[4]が3株、ウマ様G3P[8]が2株、その他が2株であった。2020年は1株でG1P[8]であった。

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