Papers - UHARA Hisashi

Detection of Epstein‐Barr Virus DNA in a Japanese Case of Lymphoepithelioma‐like Thymic Carcinoma

Yoshihiro Matsuno, Kiyoshi Mukai, Hisashi Uhara, Ichiro Akao, Shuichiroh Furuya, Yuichi Sato, Setsuo Hirohashi, Yukio Shimosato

Japanese Journal of Cancer Research   83 ( 2 ) 127 - 130  1992  [Refereed]

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Epstein‐Barr virus DNA was detected in a case of lymphoepithelioma‐like thymic carcinoma. A homogeneous terminal structure of the viral DNA was demonstrated in this case, indicating the presence of the viral genome in clonally expanded tumor cells. Since all of 26 other thymic epithelial tumors (eight non‐invasive, 13 invasive thymomas and four non‐lymphoepithelioma‐like thymic carcinomas) in Japanese were negative by polymerase chain reaction, it is suggested that lymphoepithelioma‐like thymic carcinoma may represent a unique pathological entity distinct from Epstein‐Barr virus‐negative thymic epithelial tumors, which are in the majority in Japan. Copyright © 1992, Wiley Blackwell. All rights reserved

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Detection of epstein-barr virus dna in formalin-fixed paraffin-embedded tissue of nasopharyngeal carcinoma using polymerase chain reaction and in situ hybridization

Ichiro Akao, Yuichi Sato, Kiyoshi Muka, Hisashi Uhara, Shuichiro Furuya, Tomohide Hoshikawa, Yukio Shimosato, Isamu Takeyama

Laryngoscope   101 ( 3 ) 279 - 283  1991  [Refereed]

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The polymerase chain reaction method for amplification of DNAin formalin-fixed, paraffin-embedded tissue sections was used to detect Epstein-Barr virus DNA in nasopharyngeal carcinomas from Japanese patients. Thirty-one cases of nasopharyngeal carcinoma and 8 cases of lymph node metastasis of nasopharyngeal carcinoma were studied. Detection rates of Epstein-Barr virus in various types of nasopharyngeal carcinoma according to the World Health Organization classification were as follows: 10 of 10 undifferentiated carcinomas, 8 of 13 nonkeratinizing carcinomas, and 5 of 7 keratinizing carcinomas. Eight lymph node metastases, for which the primary was positive for Epstein-Barr virus, also contained Epstein-Barr virus DNA. By in situ hybridization using a biotinylated Epstein-Barr virus probe, it was clearly demonstrated that Epstein-Barr virus DNA was localized in the nuclei of the neoplastic cells. The clinical features of nasopharyngeal carcinoma with or without Epstein-Barr virus were not different. These results demonstrate that nasopharyngeal carcinoma in Japanese patients is closely associated with Epstein-Barr virus infection, similar to nasopharyngeal carcinoma of other endemic and nonendemic areas. © The American Laryngological, Rhinological & Otological Society, Inc.

PubMed

Detection of Epstein‐Barr Virus DNA in Reed‐Sternberg Cells of Hodgkin's Disease Using the Polymerase Chain Reaction and in situ Hybridization

Hisashi Uhara, Yuichi Sato, Kiyoshi Mukai, Ichiro Akao, Yoshihiro Matsuno, Shuichiroh Furuya, Tomohide Hoshikawa, Yukio Shimosato, Toshiaki Saida

Japanese Journal of Cancer Research   81 ( 3 ) 272 - 278  1990  [Refereed]

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Thirty‐one cases of Hodgkin's disease were examined for the occurrence of Epstein‐Barr virus (EBV) genome by using the polymerase chain reaction (PCR) of DNA in formalin‐fixed paraffin‐embedded tissues and the in situ hybridization technique. The cases were subdivided into 17 cases of nodular sclerosis (NS), nine cases of mixed cellularity (MC), four cases of lymphocyte predominance (LP), and one case of lymphocyte depletion (LD). EBV DNA was detected in eight cases including four cases of NS, three cases of MC and one case of LP. The sensitivity of PCR was higher than that of Southern blot hybridization of DNA from fresh frozen tissue, because Southern blot hybridization using the BamHI‐W fragment of EBV detected virus DNA only in two of three cases which were positive by PCR. The results of in situ hybridization studies confirmed that EBV genome was localized within the nuclei of Reed‐Sternberg (RS) cells and their mononuclear variants. Furthermore, double‐labeling studies combining in situ hybridization and immunocytochemistry using CD30 (BerH2) and CD15 (LeuM1) as markers of RS cells, as well as pan B‐marker (L26) and pan T‐marker, CD45RO (UCHL1), were performed to demonstrate the phenotype of EBV DNA‐positive cells, confirming that EBV DNA was present in RS cells but not in lymphocytes. The results of this study indicate a significant association between EBV and some cases of Hodgkin's disease. Copyright © 1990, Wiley Blackwell. All rights reserved

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Detection of human papillomavirus DNA in laryngeal squamous cell carcinomas by polymerase chain reaction

Tomohide Hoshikawa, Takashi Nakajima, Hisashi Uhara, Masahiro Gotoh, Yukio Shimosato, Kouichiro Tsutsumi, Isamu Ono, Satoshi Ebihara

Laryngoscope   100 ( 6 ) 647 - 650  1990  [Refereed]

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The presence of human papillomavirus genomes-16 and -6b in metastatic cervical lymph nodes was examined in 34 cases of laryngeal carcinomas by means of polymerase chain reaction, which had been fixed in formalin and embedded in paraffin. Human papillomavirus DNAs extracted from paraffin-embedded tumor tissues were used for polymerase chain reaction with amplification of the E6 region of human papillomavirus genome-16 and the El region of human papillomavirus genome-6b. Human papillomavirus genome-16 sequences were positively amplified in six (17.6%) metastatic tumors -6b sequence was positively amplified in one (2.9%) metastatic tumor. Laryngeal carcinomas of glottic origin showed high human papillomavirus genome-16 DNA-positive rates (4 of 9 cases, 44.4%) compared to those of other sites. These results suggest that human papillomavirus genome-16 infection might be closely associated with the development of some laryngeal squamous cell carcinomas of glottic origin similar to uterine cervical carcinogenesis. © The American Laryngological, Rhinological & Otological Society, Inc.

PubMed

BOWENS DISEASE WITH INVASIVE-CARCINOMA SHOWING SWEAT GLAND DIFFERENTIATION

T SAIDA, Y OKABE, H UHARA

JOURNAL OF CUTANEOUS PATHOLOGY ( MUNKSGAARD INT PUBL LTD )  16 ( 4 ) 222 - 226  1989.08  [Refereed]

Phytohemagglutinin-binding sites in the skin: A useful histochemical marker of acrosyringium and distal portions of intradcrnial sweat ducts

Toshiaki Saida, Hisashi Uhara, Hajime Mikoshiba

Dermatology   179 ( 1 ) 25 - 28  1989  [Refereed]

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Phytohemagglutinin (PHA)-binding sites were histochemically investigated in normal adult skin and various skin tumors using peroxidase-labeled lectin. In the normal skin, acrosyringium and distal portion of intradermal duct of eccrine and apocrine sweat glands were intensely stained with PHA. Other elements of the skin were virtually negative. Among various kinds of sweat gland tumors and other skin tumors, syringoma and eccrine porocar- cinoma were intensely stained with PHA. Small ductal structures of nodular hidradenoma and mixed tumor of the skin were also stained with PHA. No other skin tumors were positive with PHA staining. The present study clearly showed that PHA is a useful histochemical marker of distal portion of eccrine and apocrine sweat ducts. © 1989 Karger AG, Basel.

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Heterophile hanganutziu-deicher antigen in ganglioside fractions of human melanoma tissues

Shigeo Kawachi, Toshiaki Saida, Hisashi Uhara, Uemura Kei-Ichi, Tamotsu Taketomi, Kyoichi Kano

International Archives of Allergy and Immunology   85 ( 3 ) 381 - 383  1988  [Refereed]

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Gangliosides were purified from melanoma tissue extracts obtained from 5 patients. GM< inf> 3< /inf> and GD< inf> 3< /inf> were identified as the major components in ganglioside fractions of the melanoma tissues. Following thin-layer chromatography, enzyme immunostaining with Hanganutziu-Deicher (H-D) antigen-specific chicken antisera demonstrated the presence of NeuGc-neolactotetraosylceramide(H-D5) and NeuGc-lacto-N-norhexa-osylceramide. © 1988 S. Karger AG, Basel.

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