Degree 【 display / non-display 】

Degree 【 display / non-display 】

• 順天堂大学大学院医学研究科感染制御科学   博士（医学）

Professional Memberships 【 display / non-display 】

Professional Memberships 【 display / non-display 】

• 2020.04

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  Now

  日本実験動物学会

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  JAPANESE SOCIETY FOR BACTERIOLOGY

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  THE JAPANESE SOCIETY OF VETERINARY SCIENCE

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  公私立大学実験動物施設協議会

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  日本ブドウ球菌研究会

Research Areas 【 display / non-display 】

Research Areas 【 display / non-display 】

• Life sciences   Biodiversity and systematics  

• Life sciences   Bacteriology  

• Life sciences   Laboratory animal science  

• Life sciences   Genomics  

• Life sciences   Veterinary medicine  

Affiliation 【 display / non-display 】

Affiliation 【 display / non-display 】

• Sapporo Medical University   School of MedicineAnimal Experimentation Center   講師  

Research Interests 【 display / non-display 】

Research Interests 【 display / non-display 】

• 獣医微生物学

• MRSA

• メタゲノム

• 糞便移植

• 哺乳類

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Papers 【 display / non-display 】

Papers 【 display / non-display 】

• Emergence of community-associated methicillin-resistant Staphylococcus aureus ΨUSA300 among Japanese people with HIV, resulted from stepwise mutations in 2010s.

  Koh Shinohara, Yuki Uehara, Katsuji Teruya, Takashi Sasaki, Tadashi Baba, Hidemasa Nakaminami, Pegah Kananizadeh, Yuh Morimoto, Yoshimi Kikuchi, Shinichi Oka

  Scientific reports   13 ( 1 ) 8322 - 8322  2023.05  [Refereed]  [International journal]

  　View Summary

  Although infection with the methicillin-resistant Staphylococcus aureus (MRSA) clone USA300 is extremely rare in Japan, the uniquely evolved clone ΨUSA300 has been reported in Japan. An outbreak of a distinct USA300 clone was recently reported in an HIV/AIDS referral hospital in Tokyo. The present study investigated the evolutionary origin and genetic diversity of USA300-related clones causing regional outbreaks among people living with HIV (PLWHIV) in Tokyo. MRSA isolates collected from PLWHIV in an HIV/AIDS referral center in Tokyo were subjected to whole-genome sequencing and their genetic features were compared with those of previously described USA300 MRSA genomes. Of the 28 MRSAs isolated in 2016-2019, 23 (82.1%) were identified as USA300, with 22 (95.6%) of the latter identified as ΨUSA300. Although the genomic structure of ΨUSA300 was identical to the structures of reference USA300 strains, one clade (cluster A) was found to have acquired 29 previously identified lineage-specific mutations in a stepwise manner. The estimated divergence dates of ΨUSA300 and Cluster A were 2009 and 2012, respectively. These findings suggested that the ΨUSA300 clone had spread among PLWHIVs in Tokyo in the early 2010s, with stepwise acquisition of lineage-specific nonsynonymous mutations.

  DOI PubMed

• Complete Genome Sequencing of a Community-Associated Methicillin-Resistant Staphylococcus aureus ψUSA300 Strain JICS127, a Uniquely Evolved USA300 Lineage in Japan.

  Koh Shinohara, Tadashi Baba, Takashi Sasaki, Katsuji Teruya, Yuki Uehara

  Microbiology resource announcements   11 ( 9 ) e0071722  2022.09  [Refereed]  [International journal]

  　View Summary

  A ψUSA300 clone of MRSA, a derivative of USA300, is uniquely found in Japan and has 12-bp deletion on ccrB2 in type IVa staphylococcal cassette chromosome mec element. We hereby present the complete genome of ψUSA300 strain JICS127.

  DOI PubMed

• Genetic and phenotypic diversity of methicillin-resistant Staphylococcus aureus among Japanese inpatients in the early 1980s.

  Hui Zuo, Yuki Uehara, Yujie Lu, Takashi Sasaki, Keiichi Hiramatsu

  Scientific reports   11 ( 1 ) 5447 - 5447  2021.03  [Refereed]  [International journal]

  　View Summary

  To trace the linkage between Japanese healthcare-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) strains in the early 1980s and the 2000s onward, we performed molecular characterizations using mainly whole-genome sequencing. Among the 194 S. aureus strains isolated, 20 mecA-positive MRSA (10.3%), 8 mecA-negative MRSA (4.1%) and 3 mecA-positive methicillin-susceptible S. aureus (MSSA) (1.5%) strains were identified. The most frequent sequence type (ST) was ST30 (n = 11), followed by ST5 (n = 8), ST81 (n = 4), and ST247 (n = 3). Rates of staphylococcal cassette chromosome mec (SCCmec) types I, II, and IV composed 65.2%, 13.0%, and 17.4% of isolates, respectively. Notably, 73.3% of SCCmec type I strains were susceptible to imipenem unlike SCCmec type II strains (0%). ST30-SCCmec I (n = 7) and ST5-SCCmec I (n = 5) predominated, whereas only two strains exhibited imipenem-resistance and were tst-positive ST5-SCCmec II, which is the current Japanese HA-MRSA genotype. All ST30 strains shared the common ancestor strain 55/2053, which caused the global pandemic of Panton-Valentine leukocidin-positive MSSA in Europe and the United States in the 1950s. Conspicuously more heterogeneous, the population of HA-MRSA clones observed in the 1980s, including the ST30-SCCmec I clone, has shifted to the current homogeneous population of imipenem-resistant ST5-SCCmec II clones, probably due to the introduction of new antimicrobials.

  DOI PubMed

• Nicotine Oral Administration Attenuates DSS-Induced Colitis Through Upregulation of Indole in the Distal Colon and Rectum in Mice.

  Akihito Nakajima, Tomoyoshi Shibuya, Takashi Sasaki, Yu Jie Lu, Dai Ishikawa, Keiichi Haga, Masahito Takahashi, Naoko Kaga, Taro Osada, Nobuhiro Sato, Akihito Nagahara

  Frontiers in medicine   8   789037 - 789037  2021  [Refereed]  [International journal]

  　View Summary

  Nicotine affects the gastrointestinal environment and modulates ulcerative colitis (UC). However, the associations among nicotine, gut metabolites, and UC are still largely unknown. We investigated whether orally administered nicotine affected gut metabolites and dextran sodium sulfate (DSS)-induced colitis. C57BL/6 male mice were orally administered nicotine solution in drinking water prior to inducing DSS-induced colitis. Short-chain fatty acids (SCFAs) and indole in gut contents and fecal samples were measured by GC-MS and hydroxylamine-based indole assays, respectively. Oral administration of nicotine increased indole concentration in feces, but, in contrast, SCFA values did not differ with nicotine administration. Indole levels were increased in the distal colon and rectum but not in the cecum and proximal colon. DSS-induced colitis was less severe clinically and histological changes were minimal in the rectum of orally nicotine-administered mice compared to mice drinking only water. 16S rRNA microbiome on the feces revealed an increasing in Clostridium and Porphyromonas in nicotine-administered mice. In conclusion, nicotine administration was associated with increased indole levels in the distal colon and rectum and attenuated DSS-induced colitis. Oral administration of nicotine may play a potential role in indole upregulation and prevention of UC.

  DOI PubMed

• Corynebacterium Species of the Conjunctiva and Nose: Dominant Species and Species-Related Differences of Antibiotic Susceptibility Profiles.

  Saichi Hoshi, Daisuke Todokoro, Takashi Sasaki

  Cornea   39 ( 11 ) 1401 - 1406  2020.11  [Refereed]  [International journal]

  　View Summary

  PURPOSE: Nondiphtherial Corynebacterium species are normal residents of human skin and mucosa, including the conjunctiva and nose, but can cause conjunctivitis and keratitis. Recently, resistance against various classes of antibiotics has been reported in Corynebacterium. The present study investigated the type of species and antibiotic susceptibilities of the conjunctival and nasal Corynebacterium species. METHODS: This study examined 183 strains of Corynebacterium species that were isolated from patients undergoing preoperative examinations for cataract surgery. Species were identified by RNA polymerase β-subunit-encoding gene (rpoB) sequencing. Antibiotic susceptibility tests were performed by the microdilution method according to the Clinical and Laboratory Standards Institute standard method M45. RESULTS: Corynebacterium macginleyi was the most predominant species (84%; 46 of 55) in the conjunctiva. The 2 major species in the nasal cavity were Corynebacterium accolens and Corynebacterium propinquum (44% and 31%, respectively), followed by Corynebacterium pseudodiphtheriticum (8%), Corynebacterium jeikeium (7%), and C. macginleyi (3%). In contrast to other nasal Corynebacterium species, only C. macginleyi showed a high susceptibility to macrolides. However, among nonconjunctival Corynebacterium species, C. propinquum, was unique in having a high resistance rate to levofloxacin (29%), comparable with that observed in C. macginleyi (36%). Penicillin G and tobramycin showed good susceptibility in almost all strains. CONCLUSIONS: Drug resistance against fluoroquinolones and macrolides was observed in Corynebacterium species, with the antibiotic susceptibility profiles correlating with differences of the species and niche. Nasal and conjunctival Corynebacterium profiles of drug resistance suggest habitat segregation strictly at the species level.

  DOI PubMed

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Books and Other Publications 【 display / non-display 】

Books and Other Publications 【 display / non-display 】

• イヌの膿皮症治療薬と薬剤耐性菌の歴史的変遷

  佐々木崇( Part： Contributor)

  第20回日本獣医皮膚科学会学術大会記念誌  2017.03

• 人獣共通感染症

  佐々木崇( Part： Contributor, ブドウ球菌感染症)

  医薬ジャーナル社  2016.02

• 感染症診断における細胞診と細菌検査

  佐々木崇( Part： Contributor)

  Small Animal Dermatology  2014.09

• 小動物臨床におけるブドウ球菌同定の実際

  佐々木崇( Part： Contributor)

  MVM (Journal of Modern Veterinary Medicine)  2009.11

Misc 【 display / non-display 】

Misc 【 display / non-display 】

• 犬の表在性膿皮症におけるクロルヘキシジン外用療法とセファレキシン全身療法の治療効果比較

  佐々木崇, 山崎真大, 原田和記, 伊従慶太, 山岸建太郎, 永田雅彦, 西藤公司, 加納塁

  日本獣医皮膚科学会学術大会・総会   27th  2024

  J-GLOBAL

• 図解 日本獣医皮膚科学会推進研究の紹介 イヌ表在性膿皮症の一次診療症例に対するクロルヘキシジン外用療法およびセファレキシン全身療法の治療効果比較

  佐々木 崇

  獣医臨床皮膚科 ( (一社)日本獣医皮膚科学会 )  29 ( 2 ) 87 - 95  2023.06

• 図解 日本獣医皮膚科学会推進研究の紹介 イヌ表在性膿皮症一次診療症例の薬剤耐性動向

  佐々木 崇

  獣医臨床皮膚科 ( (一社)日本獣医皮膚科学会 )  29 ( 1 ) 17 - 25  2023.03

• イヌの表在性膿皮症におけるクロルヘキシジン外用治療法およびセファレキシン全身療法の治療効果比較~2022年の研究成果と今後の目標~

  山崎真大, 佐々木崇, 原田和記, 伊從慶太, 山岸建太郎, 西藤公司, 永田雅彦

  日本獣医皮膚科学会学術大会・総会   26th  2023

  J-GLOBAL

• イヌの表在性膿皮症におけるクロルヘキシジン外用治療法およびセファレキシン全身療法の治療効果比較~2021年の研究成果と今後の目標~

  山崎真大, 佐々木崇, 原田和記, 伊從慶太, 山岸建太郎, 西藤公司, 永田雅彦

  日本獣医皮膚科学会学術大会・総会   25th  2022

  J-GLOBAL

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Awards 【 display / non-display 】

Awards 【 display / non-display 】

• 平成２３年度獣医臨床皮膚科優秀論文賞

  2012.03   日本獣医皮膚科学会   健康な犬におけるメチシリン耐性Staphylococcus pseudintermedius（MRSP）の疫学調査

  Winner： 須藤哲長, 寺井陽子, 三枝早苗, 椿下早絵, 佐々木崇, 平松啓一

• 平成22年度獣医臨床皮膚科優秀論文賞

  2011.03   日本獣医皮膚科学会   臨床検査機関でメチシリン耐性黄色ブドウ球菌（MRSA）と同定された犬由来ブドウ球菌株の分類学的再検討

  Winner： 笠井智子, 三枝早苗, 佐々木崇

Research Projects 【 display / non-display 】

Research Projects 【 display / non-display 】

• イヌ表在性膿皮症におけるセファレキシンへの原点回帰：多剤耐性菌MRSP制御戦略

  基盤研究(C)

  Project Year :

  2023.04

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  2026.03

   

  佐々木 崇, 西藤 公司, 山崎 真大, 原田 和記

• Study of inhibitory mechanism of feline infectious peritonitis virus proliferation by calcium antagonist

  Grant-in-Aid for Scientific Research (B)

  Project Year :

  2021.04

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  2024.03

   

  田中 良和, 落合 和彦, 小熊 圭祐, 佐々木 崇, 古谷 哲也, 手嶋 隆洋

• Characteristic analysis of canine mesenchymal stem cells capable of differentiating into insulin-producing cells and establishment of transplantation therapy

  Grant-in-Aid for Scientific Research (B)

  Project Year :

  2021.04

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  2024.03

   

  手嶋 隆洋, 高野 貴士, 道下 正貴, 佐々木 崇

  　View Summary

  本年度は、①優れた分化誘導効率を発揮する脂肪由来間葉系幹細胞の細胞集団の同定と②インスリン産生細胞への分化誘導効率を高める遺伝子の解明を計画した。①の解析については、脂肪由来間葉系幹細胞を表面抗原の発現様式からポピュレーション分類を行い、それぞれのグループについて、インスリン産生細胞への分化誘導を実施した。その結果、表面抗原の発現様式によるポピュレーション分類では、インスリン産生細胞への分化誘導効率に明らかな差はみられず、間葉系幹細胞をバルクで分化誘導した結果と同等の誘導効率であった。そのため、表面抗原の発現パターンから分化誘導効率に優れた細胞集団を特定することは困難であると考えられた。 ②の解析については、これまでの研究成果から数種類の候補遺伝子が特定できているため、本年度はこれらの遺伝子導入によって分化誘導効率に変化が生じるのかを検討した。初めに、候補遺伝子それぞれのプラスミドDNAを作製し、間葉系幹細胞への遺伝子導入を試みたが、導入効率が著しく低い結果であったため、プラスミドDNAによる遺伝子導入は適さないと判断し、アデノウイルスベクターを利用した遺伝子導入に変更した。数種のアデノウイルスベクターによる遺伝子導入効率を比較したうえで、候補遺伝子のアデノウイルスベクターを作製し、インスリン産生細胞へと分化誘導を試みた。その結果、膵β細胞への分化に関与する転写因子群を遺伝子導入することで、分化誘導効率の向上が確認できた。

• Analysis of diversity in tyrosine kinase inhibitor resistance in mast cell tumors toward establishment of individualized therapy

  Grant-in-Aid for Scientific Research (B)

  Project Year :

  2019.04

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  2022.03

   

  Bonkobara Makoto

  　View Summary

  This study indicates that accumulation of KIT mutations due to TK inhibitor exposure is important for TK inhibitor resistance in mast cell tumors, which results in TK inhibitor-resistant secondary mutations in KIT. In addition, it was shown that ultra-trace amounts of TK inhibitor-resistant clones may pre-exist in tumor tissues. On the other hand, although various mutations of KIT are detected in canine mast cell tumors, it was not possible to predict whether or not TK inhibitor resistance would occur based solely on the site and type of mutation. Therefore, it was considered that development of highly accurate and extensive genetic analysis system, such as next-generation sequencing analysis, for detection of KIT mutation and further functional characterization the mutant KIT proteins are important in order to establish individualized therapies to overcome TK inhibitor resistance.

• Fecal microbiota transplantation for ulcerative colitis

  Grant-in-Aid for Scientific Research (C)

  Project Year :

  2016.04

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  2019.03

   

  Ishikawa Dai

  　View Summary

  This was an open label, non-randomized, prospective control study. These patients were diagnosed with active UC, with a Lichtiger’s Clinical Activity Index (CAI) of 5 or more, or with an endoscopic Mayo clinic score of 1 or more, between July 2014 and March 2017. Results: Patients with mild-to-severe active UC (n=55 A-FMT; n=37 AFM) were included in this assessment. Seventy-nine patients completed this assessment (n=46 A-FMT; n=32 AFM). At 4 weeks after treatment, clinical responses in A-FMT were observed in 31 patients {Per Protocol Set (PPS):67.3%}, which was higher than in AFM (PPS:56.2%). Clinical remission was also observed to be higher in A-FMT than in AFM (A-FMT41.3%, AFM18.7%; p=0.06). Endoscopic sum score was associated with clinical responses (Responders7.5±3.2, Non-responders5.1±3.6; p=0.03), and clinical responses and remission were significantly higher in proctitis than in other types of colitis in A-FMT (n=38, 8; p=0.03, p=0.005).

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Presentations 【 display / non-display 】

Presentations 【 display / non-display 】

• 抗菌薬外用剤は意味があるのか？

  佐々木崇  [Invited]

  第6回日本獣医耳科研究会 特別講演 

  Presentation date： 2014.10

• 伴侶動物におけるブドウ球菌感染症の実態

  佐々木崇  [Invited]

  第59回日本ブドウ球菌研究会 教育講演 

  Presentation date： 2014.08

• 皮膚科臨床への微生物学的貢献 〜抗菌薬からマイクロバイオームまで〜

  佐々木崇  [Invited]

  第20回日本獣医皮膚科学会学術大会総会 特別講演 

  Presentation date： 2017.03

Teaching Experience 【 display / non-display 】

Teaching Experience 【 display / non-display 】

• 免疫学  

  札幌医学技術福祉歯科専門学校  

  2021.09

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  Now

   

• 動物実験の基礎知識  

  札幌医科大学  

  2017.05

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  Now

   

• 微生物学  

  埼玉大学  

  2016.12

  　

  　

   

• 遺伝子検査学実習  

  文京学院大学  

  2016

  　

  　

   

• 微生物実習、微生物学  

  順天堂大学  

  2014.04

  -

  2017.03

   

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Committee Memberships 【 display / non-display 】

Committee Memberships 【 display / non-display 】

• 2020.04

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  Now

    実験動物感染症対策委員会 委員

• 2020.04

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  Now

    バイオセーフティ委員会 委員長

• 2017.05

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  Now

    幹事

• 2017.05

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  2020.03

    バイオセーフティー委員会 委員

• 2017.04

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  Now

    犬の膿皮症治療ガイドライン策定に向けた学会推進研究班 班長

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