Details of a Researcher - ITAGAKI Shiro

ITAGAKI Shiro

写真a

Affiliation	Collaboration Center for Community and Industry
Job title	Special-Appoingment Associate Professor

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Education 【 display / non-display 】

2003

　

　

Hokkaido University 薬学研究科医療薬学専攻博士課程中退
2001

　

　

Hokkaido University 薬学研究科医療薬学専攻修士課程修了
1999

　

　

Hokkaido University Faculty of Pharmaceutical Sciences 総合薬学科卒業

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Degree 【 display / non-display 】

北海道大学博士（薬学）

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Research Experience 【 display / non-display 】

2018.04

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Now

Sapporo Medical University 特任准教授
2017.04

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Now

Sapporo Medical University 産学連携コーディネーター

産学連携コーディネーター
2015.10

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2017.03

Hirosaki University （教育研究院・医学系）准教授

准教授
2013.07

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2017.03

Hirosaki University University Hospital 副センター長(兼務)

副センター長(兼務)
2010.04

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2017.03

Hirosaki University University Hospital 副薬剤部長(併任)

副薬剤部長(併任)

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Professional Memberships 【 display / non-display 】

　

　

　

日本薬学会
　

　

　

medU－net
　

　

　

北海道医療福祉産業研究会

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Research Areas 【 display / non-display 】

Humanities & social sciences Home economics, lifestyle science
Life sciences Nutrition and health science
Humanities & social sciences Education - general
Life sciences Medical and welfare engineering

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Affiliation 【 display / non-display 】

Sapporo Medical University その他部局等特任准教授

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Research Interests 【 display / non-display 】

transporter
医療安全
予防医学
ポリフェノール
臨床研究

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Papers 【 display / non-display 】

Exploration of candidate odorants derived from patient skin lesions using gas chromatography-mass spectrometry

Ryosuke IIZAWA, Masami HORIGUCHI, Hisashi UHARA, Yuji KAN, Kunihiro TOGASHI, Makoto SUZUKI, Takafumi NOMURA, Shiro ITAGAKI, Tomoko UNO, Madoka NAKAMURA

Journal of Japan Association on Odor Environment ( Japan Association on Odor Environment ) 55 ( 5 ) 315 - 318 2024.09 [Refereed]

DOI
A simple, dual direct expression plasmid system in prokaryotic and mammalian cells.

Manabu Murakami, Agnieszka M Murakami, Manabu Yonekura, Ichiro Miyoshi, Shirou Itagaki, Yasutaka Niwa

PNAS nexus 2 ( 5 ) pgad139 2023.05 [International journal]

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We introduce a simple, dual direct cloning plasmid system (pgMAX-II) for gene expression analysis in both prokaryotic (Escherichia coli) and mammalian cells. This system, which uses a prokaryotic expression unit adapted from the pgMAX system and a mammalian promoter, is effective for subcloning using the DNA topoisomerase II toxin CcdB. Given that molecular biological cloning systems broadly rely on E. coli for rapid growth, the proposed concept may have wide applicability beyond mammalian cells.

DOI PubMed
Rapid method for plasmid DNA recombination (Murakami-system).

Agnieszka M Murakami, Manabu Yonekura, Katsuhiro Nagatomo, Yasutaka Niwa, Shirou Itagaki, Manabu Murakami

MethodsX 10 102167 - 102167 2023 [International journal]

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DNA recombination is a useful technology for cloning and subsequent functional analysis, while standard techniques for plasmid DNA recombination have remained unchanged. In the present study, we introduced rapid method for plasmid DNA recombination, which we named "Murakami-system", to complete the experiments in under 33 h. For this purpose, we selected the following: PCR amplification with 25 cycles and E. coli strain with rapid growth (incubation time of 6-8 h). In addition, we selected rapid plasmid DNA purification (mini-prep; ∼10 min) and rapid restriction enzyme incubation (20 min). This recombination system enabled rapid plasmid DNA recombination within 24-33 h, which could be useful in various fields. We also established a 1-day method for competent cell preparation. Our rapid recombination system allowed several sessions of plasmid DNA recombination to be performed every week, which improves the functional analysis of various genes.•"Rapid method for plasmid DNA recombination (Murakami-system).•E. coli strain with rapid growth (incubation time of 6-8 h).•Combination of rapid protocols (PCR, electrophoresis, DNA purification, ligation, and mini-prep) enabled plasmid DNA recombination within 24-33 h.

DOI PubMed
Enhanced β-adrenergic response in mice with dominant-negative expression of the PKD2L1 channel

Manabu Murakami, Agnieszka M. Murakami, Takayuki Nemoto, Takayoshi Ohba, Manabu Yonekura, Yuichi Toyama, Hirofumi Tomita, Yasushi Matsuzaki, Daisuke Sawamura, Kazuyoshi Hirota, Shirou Itagaki, Yujiro Asada, Ichiro Miyoshi

PLOS ONE ( Public Library of Science (PLoS) ) 17 ( 1 ) e0261668 - e0261668 2022

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Polycystic kidney disease (PKD) is the most common genetic cause of kidney failure in humans. Among the various PKD-related molecules, PKD2L1 forms cation channels, but its physiological importance is obscure. In the present study, we established a transgenic mouse line by overexpressing the dominant-negative form of the mouse PKD2L1 gene (i.e., lacking the pore-forming domain). The resulting PKD2L1del-Tg mice exhibited supraventricular premature contraction, as well as enhanced sensitivity to β-adrenergic stimulation and unstable R-R intervals in electrocardiography. During spontaneous atrial contraction, PKD2L1del-Tg atria showed enhanced sensitivity to isoproterenol, norepinephrine, and epinephrine. Action potential recording revealed a shortened action potential duration in PKD2L1del-Tg atria in response to isoproterenol. These findings indicated increased adrenergic sensitivity in PKD2L1del-Tg mice, suggesting that PKD2L1 is involved in sympathetic regulation.

DOI
Attenuated β-adrenergic response in calcium/calmodulin-dependent protein kinase IV-knockout mice.

Manabu Murakami, Agnieszka M Murakami, Yasushi Matsuzaki, Daisuke Sawamura, Takayoshi Ohba, Ichirou Miyoshi, Shirou Itagaki, Hiroyuki Sakagami

PloS one 16 ( 4 ) e0249932 2021 [Refereed] [International journal]

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In the present study, we examined the importance of Ca2+/calmodulin-dependent protein kinase IV (CaMKIV) in the regulation of cardiac function using genetically modified CaMKIV-null mice. RT-PCR analysis revealed decreased expression of voltage-dependent calcium channels in the cardiac myocytes of CaMKIV-null mice compared with wild-type mice. CaMKIV-null mice showed shortened QT time on electrocardiograms. Pharmacological analysis revealed decreased responsiveness to the β-adrenergic blocker propranolol in CaMKIV-null mice, whereas the plasma norepinephrine level was not affected. CaMKIV-null mice showed decreased baroreflex on electrocardiograms. Heart rate variability analysis showed unstable R-R intervals, a decreased low frequency power/high frequency power (LF/HF) ratio, and increased standard deviation of the normal to normal R-R intervals (SDNN) in CaMKIV-null mice, suggesting decreased responsiveness to β-adrenergic stimulation in CaMKIV-null mice. Atrial contraction analysis and cardiac action potential recording showed a decreased response to the β-adrenoceptor agonist isoproterenol in CaMKIV-null mice. Furthermore, fluorescence imaging in a CRE-hrGFP assay revealed a decreased response to isoproterenol in CaMKIV-null cardiac myocytes. Taken together, our data strongly suggest a significant effect of CaMKIV gene ablation on cardiac β-adrenergic signal transduction.

DOI PubMed

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Research Projects 【 display / non-display 】

交通事故医療の質の向上に資する、間欠動作機能を備える革新的低圧吸引器の開発

Project Year :

2024

　

　

板垣史郎

Authorship： Principal investigator
札幌医科大学医療機器等関連産業参入研修会を通じた道内地場企業との製品開発

Project Year :

2024

　

　

板垣史郎

Authorship： Principal investigator
DDSがアニサキス感染から身を守る: 萌芽から開拓への新展開

挑戦的研究(開拓)

Project Year :

2023

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2025

丁野純男
血流感染症特異的治療薬の開発に向けた大規模スクリーニングとｖｉｖｏＥＦ阻害剤ライブラリーの構築

プロジェクト支援型（START）

Project Year :

2021

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2023

佐藤豊孝
従来の抗菌薬開発法にとらわれない、新たな細菌感染症治療薬のスクリーニングに関する研究開発

Project Year :

2019

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2021

佐藤豊孝

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研究開発分担者 2019年12月6日札幌医科大学プレスリリース停滞していた抗菌薬開発に光明！これまでにない新たなスクリーニング法の構築がAMED令和元年度「創薬基盤推進研究事業」に採択されました https://web.sapmed.ac.jp/jp/news/photo/jmjbbn000000ky3l.html https://web.sapmed.ac.jp/jp/news/press/jmjbbn000000kxhc.html