Papers - Tomoko UNO

赤ワイン凍結乾燥品の長期投与による食餌性高コレステロール血症ラット血管機能の変化

清水良美, 五十嵐祐子, 利美賀子, 会田智美, 下田智子, 石幡明, 片野由美

未病システム学会雑誌     192  2008  [Refereed]

Progression of severe atherosclerosis and increased arterial pulse pressure in the newly developed heritable mixed hyperlipidaemic rabbits

T Shimoda, A Ishihata, T Aita, M Kaga, T Ito, K Ohwada, H Tomoike, Y Katano

CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY ( BLACKWELL PUBLISHING )  33 ( 3 ) 221 - 226  2006.03  [Refereed]

　View Summary

We have recently segregated a new line of rabbit, named TGH, with severely high levels of plasma triglyceride and cholesterol. The aim of the present study was to investigate the progression of atherosclerosis and haemodynamic parameters in TGH rabbits. Japanese white (JW) and TGH rabbits (24-27 months old) were anaesthetized with ketamine and xylazine. Plasma concentrations of triglyceride were 63.1 8.0 and 446.0 35.2 mg/dL in JW and TGH rabbits, respectively. Blood pressure was measured by a catheter implanted in the femoral artery. Histological examinations were performed using haematoxylin-eosin and elastica-Masson trichrome staining to detect atherosclerotic lesions. The JW rabbits had no atherosclerotic lesions. In TGH rabbits, severe atherosclerotic lesions were observed throughout the aorta, especially in the aortic arch. Basal femoral arterial pressure was not significantly different between JW and TGH rabbits. However, the basal pulse pressure in TGH rabbits (48.3 4.5 mmHg) was significantly greater than that of JW rabbits (28.0 5.6 mmHg). Intravenous infusion of N-G-nitro-L-arginine methyl ester (L-NAME; 26.9 mg/kg) increased the blood pressure of TGH and JW rabbits. There was no significant difference in the response to L-NAME between the two rabbit strains. The present study shows that severe atherosclerotic changes develop in TGH rabbits and suggests that the hyperlipidaemia combined with hypercholesterolaemia and hypertriglyceridaemia is an important factor for promoting atherosclerosis in TGH rabbits. The greater pulse pressure in TGH rabbits may be due to the increased vascular stiffness with atherosclerosis. This newly developed TGH rabbit line of heritable hypertriglyceridaemia with hypercholesterolaemia will become a useful animal model for studies on the role of hyperlipidaemia in the progression of atherosclerosis and in many atherosclerosis-related diseases.

DOI PubMed

Progression of Atherosclerosis and Femoral Arterial Blood Pressure in Heritable Hypertriglyceridemic Rabbits

Shimoda Tomoko, Ishihata Akira, Ito Tunekata

Bulletin of the Yamagata University. Medical science ( 山形大学 )  23 ( 1 ) 17 - 32  2005.02  [Refereed]

　View Summary

[Objective] It has been reported that hypercholesterolemia plays a role in progression of atherosclerosis. Watanabe heritable hyperlipidemic rabbits have been used widely as a model of familial hypercholesterolemia with atherosclerosis, while the emerging data raised a possibility of an important role of hypertriglyceridemia in the pathogenesis of atherosclerosis. We have recently segregated a new line with severely high (TGH) and moderately high (TGL) levels of plasma triglyceride. The hemodynamic parameters of TGH and TGL are not defined. The aim of present study was to examine the progre...

CiNii

Extracts of Red Wine with Polyphenol Compounds Induce Endothelium-Dependent Vascular Relaxation in Aged Rat Aorta

Kaga Mikako, Ishihata Akira, Aita Tomomi

Bulletin of the Yamagata University. Medical science ( Yamagata University )  23 ( 1 ) 33 - 41  2005.02  [Refereed]

　View Summary

[Objective] The vasorelaxing effects of the red wine extracts were studied in rat thoracic aorta. [Methods] Aortas were isolated from young (2-3 months) and aged (27 months) F344 rats and the ring preparations with or without endothelium were mounted in Krebs-Henseleit solution to measure the developed tension. The polyphenolic substances were added cumulatively after the contraction with phenylephrine.[Results] Extracts of red wine relaxed the vessels concentration-dependently. These vasorelaxant effects were attenuated by endothelial removal. In young rat, vasorelaxation induced by RWPC was significantly inhibited by L-NNA (100 μM), diclofenac (10 μM) and 30mM KCl. In aged rat, the inhibition by L-NNA and diclofenac was less than that of young rat, and 30mM KCl potently decreased the relaxation. However, the vasorelaxant responses were preserved even in aged aorta.[Conclusion] RWPC induces vascular relaxation endothelium-dependently not only in young rat but also in aged rat. The relaxation was mediated via NO, prostacyclin and EDHF in young aorta, while EDHF may play an important role in aged aorta.

CiNii