Organization
School of Medicine Department of Perinatal Medicine Assistant Professor
Title
Assistant Professor
External link
SAKAI Takurou
Research Areas
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Life Science / Neurosurgery
Research History
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Sapporo Medical University Graduate School of Medicine
2014
Research Projects
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Intravenous infusion of exosome for the treatment of neonatal hypoxic ischemic encephalopathy
Grant number：23K07338 2023.4 - 2026.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Grant amount：\4680000 （ Direct Cost: \3600000 、 Indirect Cost：\1080000 ）
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Mesenchymal stem cells for brain injury of prematurity in a rat model.
Grant number：20K08163 2020.4 - 2023.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Grant amount：\4290000 （ Direct Cost: \3300000 、 Indirect Cost：\990000 ）
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The development of novel therapies for intractable epilepsy with bone marrow mesenchymal stem cells
Grant number：16K09993 2016.4 - 2019.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Fukumura Shinobu
Grant amount：\4680000 （ Direct Cost: \3600000 、 Indirect Cost：\1080000 ）
The present study tested the hypothesis that systemically infused mesenchymal stem cells (MSCs) reduce epileptogenesis by inhibiting neuronal cell death and suppressing aberrant MFS, leading to preservation of cognitive function in a rat model of epilepsy. Status epilepticus (SE) was induced using the lithium-pilocarpine injection. MSC infusion inhibited epileptogenesis and preserved cognitive function after SE. The infused MSCs preserved GAD67+ and NeuN+ hippocampal neurons. Furthermore, the MSC infusion suppressed the aberrant MFS in the hippocampus as evidenced by manganese enhanced MRI and Timm staining. This study demonstrated that the intravenous infusion of MSCs mitigated epileptogenesis, thus advancing MSCs as an effective approach for epilepsy in clinical practice.
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Therapeutic Effects of Intravenous Infusion of MSCs in Hypoxic-Ischemic Encephalopathy Rat
Grant number：26462168 2014 - 2016
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Sakai Takuro, HONMOU Osamu
Authorship：Principal investigator Grant type：Competitive
Grant amount：\4160000 （ Direct Cost: \3200000 、 Indirect Cost：\960000 ）
We aimed to investigate the effect of mesenchymal stem cells (MSCs) transplantation in a rat model of neonatal hypoxic-ischemic encephalopathy (HIE) by histological, MRI, and behavioral analysis. At postnatal day 7, rats were underwent HI by left common carotid artery occlusion followed by 120 min hypoxia (8% oxygen). At postnatal day 10, transplantation of MSCs was performed by intravenous injection. Compared with the vehicle (control) group, the MSC infused group showed a significant improvement in athletic performance by the evaluation with the beam walk test. The MRI also demonstrated that the MSC infused group showed a significant reduction of the ischemic volume compared to the vehicle group. Collectively, intravenous infusion of MSCs might provide therapeutic efficacy in a rat model of HIE.
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Functional recovery following intravenous infusion of MSCs via repair of pericytes in rat spinal cord injury
Grant number：25462227 2013.4 - 2016.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Sasaki Masanori, Wanibuchi Masahiko, Mikami Takeshi, Kobayashi Masaki, Nakazaki Masahito, Sakai Takuro, Sasaki Yuichi, Suzuki Junpei, Morita Tomonori, Namioka Ai, Namioka Takahiro
Grant amount：\5070000 （ Direct Cost: \3900000 、 Indirect Cost：\1170000 ）
Mesenchymal stem cells (MSCs) derived from bone marrow may represent a potential source for organ regeneration via multiple mechanisms based on animal experiments. In this study, we induced a contusive SCI at T9 in the rat and studied the effects of intravenous MSC infusion on blood spinal cord barrier (BSCB) permeability, microvascular architecture and locomotor recover. Spatial and temporal changes in BSCB integrity were assessed by intravenous infusions of Evans blue (EvB). SCI resulted in prolonged BSCB leakage that was most severe at the impact site but disseminated extensively rostral and caudal to the lesion. Contused spinal cords also showed an increase in microvasculature. In MSC-treated rats, BSCB leakage was reduced, and locomotor function improved after MSC infusion. These results suggest that intravenously delivered MSCs have important effects on reducing BSCB leakage which could contribute to their therapeutic efficacy.
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Profile analysis of mesenchymal stem cell for cellular therapy
Grant number：25462226 2013.4 - 2016.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Honmou Osamu, Wanibuchi Masahiko, Sasaki Masanori, Mikami Takeshi, Kobayashi Masaki, Nakazaki Masahito, Sakai Takuro, Sasaki Yuichi, Suzuki Junpei, Morita Tomonori, Namioka Ai, Namioka Takahiro
Grant amount：\4940000 （ Direct Cost: \3800000 、 Indirect Cost：\1140000 ）
Recent progress in regenerative medicine for injured neural tissue have revealed that greater therapeutic efficacy can be achieved by stem cell therapy via multiple mechanisms for various disorders. Especially, it has been suggested that mesenchymal stem cells (MSCs) derived from bone marrow may represent a potential source for organ regeneration. In this study, cell biological analyses were performed with MSCs from human and rat bone marrow using several molecular, biochemical and genetics approaches. Therapeutic efficacy by rat MSCs in vivo was also examined with rat stroke model.
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Characteristic analysis of mesenchymal stem cells for regenerative medicine
Grant number：24592138 2012.4 - 2015.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
RIE Onodera, HONMOU Osamu, WANIBUCHI Masahiko, SASAKI Masanori, MIKAMI Takeshi, KOBAYASHI Masaki, NAKAZAKI Masahito, SAKAI Takurou, SASAKI Yuichi, SUZUKI Junpei, MORITA Tomonori
Grant amount：\5200000 （ Direct Cost: \4000000 、 Indirect Cost：\1200000 ）
Recent advance in regenerative medicine have demonstrated that greater therapeutic efficacy can be obtained by stem cell therapy via multiple mechanisms compared to the standard therapies for various diseases. Especially, it has been suggested that bone marrow stem cells may represent an alternative source of neural progenitor cells for organ regeneration. Among bone marrow stem cells, much attention has been paid to mesenchymal stem cells (MSCs, also referred to as bone marrow stromal cells) because of their plasticity for differentiation into classical mesenchymal lineages, such as neuronal lineages. In this study, cell biological analyses were performed with MSCs from human and rat bone marrow using several molecular, biochemical and genetics approaches. Therapeutic efficacy with rat MSCs in vivo was also examined with rat stroke model.