Affiliation 【 display / non-display 】

Affiliation 【 display / non-display 】

• Osaka City University   Graduate School of Nursing   教授  

Research Interests 【 display / non-display 】

Research Interests 【 display / non-display 】

• Pediatric Nursing

• Child Health

• Viral Infection

• Childcare

• Epidemiology

Misc 【 display / non-display 】

Misc 【 display / non-display 】

• Molecular epidemiology of astroviruses in Japan from 1995 to 1998 byreverse transcription-polymerase chain reaction with serotype-specific primers (1 to 8)

  J Med Virol.   61(3), 326-31  2000.07

  DOI

• Development of a simple and rapid latex test for rotavirus in stool samples

  Hideki Kohno, Shiho Akihara, Osamu Nishio, Hiroshi Ushihima

  Pediatrics International   42 ( 4 ) 395 - 400  2000

  Book review, literature introduction, etc.  

  　View Summary

  In the fields of infectious diseases, a variety of immunoassay methods has been reported. More simple, rapid and cost-effective manual tests for infectious diseases are needed. For the purpose of simple diagnosis of rotavirus infection, a rapid test against human rotavirus was developed. As for the preparation of the reagent, clinically isolated human rotavirus was cultured using MA-104 cells. Then, the cultured virus was purified by an ultracentrifugation method. The purified vires was inactivated with formaldehyde for use as antigen for immunization of rabbits. The antibody was purified and applied to sensitize the surface of latex particles to react with rotavirus in diarrheal stools. The latex agglutination test showed that the sensitivity was 98.2% and the specificity was 94.8%, in comparison with electron microscopy as the gold standard. Furthermore, to measure the virus concentration in stool samples, an automated latex photometric immunoassay system (LPIA) was used, which has been developed for quantitative measurement of agglutination. The range of the reactive viruses detected by the LPIA system was 3 x 107-109 virions/mL. The rotavirus-positive and rotavirus-negative subjects were clearly discriminated. The results were in good accordance with electron microscope results. Those results showed that the latex agglutination test is a good tool for the simple and rapid detection of rotavirus in stool samples.

  DOI PubMed

• Genetic variation in the VP4 and NSP4 genes of human rotavirus serotype 3(G3 type) isolated in China and Japan

  Microbiol Immunol.   43(2), 171-5  1999

  DOI

• Distribution of rotavirus VP4 genotype and VP7 serotype among Chinese children

  Acta Paediatr Jpn.   40(6), 641-3  1998