Misc - 馬場　剛

Cloning and characterization of a tumor-associated antigen, beta-casein-like protein

T Baba, M Koizumi, T Suzuki, Yamanaka, I, S Yamashita, R Kudo

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS ( ACADEMIC PRESS INC )  284 ( 2 ) 340 - 345  2001年06月

　概要を見る

Monoclonal antibody (MAb) 1C5 reacts with 87% of uterine cervical adenocarcinoma and bovine beta -casein, but not with squamous cell carcinoma. To clarify the characteristics of the antigen (beta -casein-like protein; BCLP) recognized by MAb 1C5, molecular cloning was performed using 5 ' rapid amplification of the cDNA ends (5 ' RACE) and the oligo-capping method. The protein predicted from the cDNA consisting of 937 nucleotides comprises 222 amino acids. The BCLP gene and deduced amino acid sequences were novel and showed no similarity to known cancer-associated genes in the database. Northern blot analysis showed that a 1.1 kb transcript was ubiquitously expressed in cancer cell lines and was predominantly expressed in uterine cervical adenocarcinoma. To clarify the function of BCLP, BCLP cDNA was transfected into L929 cells, resulting in a significant increase in cell area, a downregulation of cell growth rate and a decrease in cell attachment. We conclude that BCLP might be associated with cell morphology and a regulation of growth pattern of tumor. (C) 2001 Academic Press.

DOI PubMed CiNii

Surgical management of genital prolapse: Is chain cystourethrography useful for evaluating anatomical corrections and urinary symptoms after surgery?

T. Baba, M. Koizumi, R. Tanaka, S. Yamashita, M. Noda, I. Yamanaka, T. Suzuki, E. Ito, R. Kudo

Journal of Obstetrics and Gynaecology Research ( University of Tokyo Press )  26 ( 4 ) 289 - 294  2000年

　概要を見る

Objective: To evaluate the relationship between chain cystourethrography and surgery for genital prolapse and urinary symptoms. Methods: A retrospective study of women with genital prolapse was conducted from January 1991 through December 1997. To assess the preoperative and postoperative anatomical situations objectively, chain cystourethrography was performed. Results: Fifty-seven patients underwent surgical repairs for genital prolapse. All of them recovered from the genital prolapse both subjectively and objectively after surgery. Chain cystourethrography is useful for evaluating postoperative anatomical corrections. However, 6 patients (12%) suffered from a recurrent feeling of genital prolapse, and 14 patients (28%) had urinary symptoms postoperatively. Conclusions: Our treatment showed good anatomical corrections based on chain cystourethrography, and there were no prognostic differences among the surgical procedures. However, anatomical correction does not always mean improvement of urinary symptoms. Further studies are needed to clarify what factors contribute to the prognosis in such cases.

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A case of Stage-IVb cervical adenocarcinoma successfully treated by combination chemotherapy: Case report

Masaki Takehara, Yoshihiro Nishioka, Tsuyoshi Saito, Tsuyoshi Baba, Takahiro Suzuki, Eiki Ito, Ryuichi Kudo

Journal of Obstetrics and Gynaecology Research ( University of Tokyo Press )  26 ( 2 ) 133 - 136  2000年

　概要を見る

A 54-year-old patient with a Stage IVb adenocarcinoma of the cervix was treated with combination chemotherapy. This regimen consisted of intravenous cisplatin (70 mg/m2) and aclacinomycin A (30 mg/m2) on Day 1, followed by mitomycin C (5 mg/m2) on Day 2 and 3. A pathologically complete response was achieved by this regimen. The patient is well and has been free of symptoms for 66 months.

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