論文 - 久原　真

小脳性運動失調を主徴とした抗contactin-1(CNTN1)抗体陽性nodopathyの一例

河田 由香, 池田 和奈, 種本 真将, 横川 和樹, 山田 稔, 齋藤 太郎, 岩原 直敏, 松村 晃寛, 鈴木 秀一郎, 緒方 英紀, 磯部 紀子, 久原 真

臨床神経学 ( (一社)日本神経学会 )  63 ( 11 ) 778 - 778  2023年11月

小脳性運動失調を主徴とした抗contactin-1(CNTN1)抗体陽性nodopathyの一例

河田 由香, 池田 和奈, 種本 真将, 横川 和樹, 山田 稔, 齋藤 太郎, 岩原 直敏, 松村 晃寛, 鈴木 秀一郎, 緒方 英紀, 磯部 紀子, 久原 真

臨床神経学 ( (一社)日本神経学会 )  63 ( 11 ) 778 - 778  2023年11月

神経筋疾患の問題症例 亜急性に進行した小脳性運動失調症と脱髄性ニューロパチーの一例

河田 由香, 池田 和奈, 山田 稔, 齋藤 太郎, 津田 玲子, 鈴木 秀一郎, 緒方 英紀, 磯部 紀子, 久原 真

臨床神経生理学 ( (一社)日本臨床神経生理学会 )  51 ( 5 ) 508 - 508  2023年10月

発症早期の自己免疫性てんかんの臨床的特徴の検討

種本 真将, 横川 和樹, 齋藤 太郎, 岩原 直敏, 松村 晃寛, 鈴木 秀一郎, 山本 彬広, 外山 祐一郎, 古山 裕康, 溝渕 雅広, 佐光 一也, 久原 真

臨床神経学 ( (一社)日本神経学会 )  63 ( Suppl. ) S321 - S321  2023年09月

クリーゼ中に胸腺摘除術を施行し良好な転帰が得られた胸腺腫合併抗AChR抗体陽性全身型重症筋無力症の一例

河田 由香, 種本 真将, 山田 稔, 横川 和樹, 齋藤 太郎, 岩原 直敏, 松村 晃寛, 鈴木 秀一郎, 久原 真, 高瀬 貴章, 宮島 正博, 渡辺 敦

臨床神経学 ( (一社)日本神経学会 )  63 ( 5 ) 325 - 325  2023年05月

高齢発症の抗GluR抗体陽性脳炎にリツキシマブ療法が効果を示した1例

種本 真将, 山本 彬広, 河田 由香, 横川 和樹, 山田 稔, 齋藤 太郎, 岩原 直敏, 松村 晃寛, 鈴木 秀一郎, 高橋 幸利, 久原 真

臨床神経学 ( (一社)日本神経学会 )  63 ( 5 ) 325 - 325  2023年05月

クリーゼ中に胸腺摘除術を施行し良好な転帰が得られた胸腺腫合併抗AChR抗体陽性全身型重症筋無力症の一例

河田 由香, 種本 真将, 山田 稔, 横川 和樹, 齋藤 太郎, 岩原 直敏, 松村 晃寛, 鈴木 秀一郎, 久原 真, 高瀬 貴章, 宮島 正博, 渡辺 敦

臨床神経学 ( (一社)日本神経学会 )  63 ( 5 ) 325 - 325  2023年05月

Anti-signal Recognition Particle Antibody-positive Immune-mediated Myopathy after mRNA-1273 SARS-CoV-2 Vaccination.

Masanobu Tanemoto, Ryosuke Oda, Yuichiro Toyama, Hirohiko Shizukawa, Kazuki Yokokawa, Taro Saito, Syuuichirou Suzuki, Akihiro Matsumura, Shin Hisahara

Internal medicine (Tokyo, Japan)   61 ( 23 ) 3605 - 3609  2022年12月  [国内誌]

　概要を見る

A 26-year-old Japanese woman developed a fever, myalgia and gait disturbance one day after receiving the second dose of the mRNA-1273 severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine. A neurological examination revealed symmetrical weakness and myalgia in proximal lower limbs, and a blood examination showed prominent elevation of creatinine kinase. Magnetic resonance imaging (MRI) revealed a high signal intensity in the thigh muscles on short-tau inversion recovery images, and antibody testing revealed positive findings for anti-signal recognition particle (SRP) antibody. Thus, anti-SRP antibody-positive immune-mediated myopathy was diagnosed. We initiated immunotherapy, and she was ultimately able to walk stably.

DOI PubMed

脱髄性ニューロパチーを認めたサルコイドーシスの1例

河田 由香, 種本 真将, 山田 稔, 横川 和樹, 齋藤 太郎, 岩原 直敏, 松村 晃寛, 鈴木 秀一郎, 久原 真, 松浦 洋介

臨床神経学 ( (一社)日本神経学会 )  62 ( 12 ) 975 - 975  2022年12月

てんかん発作で発症した自己免疫性神経疾患8症例の検討

種本 真将, 横川 和樹, 齋藤 太郎, 松村 晃寛, 鈴木 秀一郎, 久原 真

臨床神経学 ( (一社)日本神経学会 )  62 ( 12 ) 978 - 978  2022年12月

mRNA-1273 SARS-CoV-2ワクチン接種後に発症した抗SRP抗体陽性ミオパチーの1例

種本 真将, 鈴木 秀一郎, 松村 晃寛, 横川 和樹, 久原 真, 静川 裕彦

神経治療学 ( (一社)日本神経治療学会 )  39 ( 6 ) S263 - S263  2022年10月

脳神経内科外来のパーキンソン病患者における便秘の有訴者数と臨床像

鈴木 秀一郎, 河田 由香, 種本 真将, 池田 和奈, 横川 和樹, 松村 晃寛, 久原 真

神経治療学 ( (一社)日本神経治療学会 )  39 ( 6 ) S288 - S288  2022年10月

Activation of SIRT1 promotes membrane resealing via cortactin

Naotoshi Iwahara, Kuya Azekami, Ryusuke Hosoda, Iyori Nojima, Shin Hisahara, Atsushi Kuno

Scientific Reports ( Springer Science and Business Media LLC )  12 ( 1 )  2022年09月

　概要を見る

Abstract Muscular dystrophies are inherited myopathic disorders characterized by progressive muscle weakness. Recently, several gene therapies have been developed; however, the treatment options are still limited. Resveratrol, an activator of SIRT1, ameliorates muscular function in muscular dystrophy patients and dystrophin-deficient mdx mice, although its mechanism is still not fully elucidated. Here, we investigated the effects of resveratrol on membrane resealing. We found that resveratrol promoted membrane repair in C2C12 cells via the activation of SIRT1. To elucidate the mechanism by which resveratrol promotes membrane resealing, we focused on the reorganization of the cytoskeleton, which occurs in the early phase of membrane repair. Treatment with resveratrol promoted actin accumulation at the injured site. We also examined the role of cortactin in membrane resealing. Cortactin accumulated at the injury site, and cortactin knockdown suppressed membrane resealing and reorganization of the cytoskeleton. Additionally, SIRT1 deacetylated cortactin and promoted the interaction between cortactin and F-actin, thus possibly enhancing the accumulation of cortactin at the injury site. Finally, we performed a membrane repair assay using single fiber myotubes from control and resveratrol-fed mice, where the oral treatment with resveratrol promoted membrane repair ex vivo. These findings suggest that resveratrol promotes membrane repair via the SIRT1/cortactin axis.

DOI

Ubiquitin-dependent rapid degradation conceals a cell-protective function of cytoplasmic SIRT3 against oxidative stress

Takashi Hayashi, Takashi Matsushita, Shin Hisahara, Naotoshi Iwahara, Atsushi Kuno, Risa Kunimoto, Ryusuke Hosoda, Masaya Tanno, Shun Shimohama, Yoshiyuki Horio

The Journal of Biochemistry ( Oxford University Press (OUP) )  171 ( 2 ) 201 - 213  2022年02月

　概要を見る

Abstract SIRT3 is an NAD+-dependent protein deacetylase localized in mitochondria. Several studies reported localization of SIRT3 in the cytoplasm or nucleus, but data of these studies were not consistent. We detected expression of mitochondrial (SIRT3mt) and cytoplasmic (SIRT3ct) Sirt3 mRNAs in the mouse brain, and we also found SIRT3 immunostaining of mitochondria and cytoplasm in the brain and cultured neural cells. However, expression levels of SIRT3ct in COS cells transfected with SIRT3ct cDNA were much lower than those of SIRT3mt. We found that SIRT3ct but not SIRT3mt was promptly degraded by ubiquitin-dependent degradation, in which SIRT3ct degradation was mediated mainly by ubiquitination of NH2-terminal methionine and partly by that of lysine residues of SIRT3ct. SIRT3ct expression level was significantly enhanced by the treatment of cells with staurosporine or H2O2. H2O2 treatment promoted nuclear translocation of SIRT3ct and induced histone H3 deacetylation and superoxide dismutase 2 expression. Overexpression of SIRT3ct decreased cell death caused by H2O2 at levels similar to those achieved by overexpression of SIRT3mt. Knockdown of Sirt3 mRNA increased cell death caused by amyloid-β (Aβ), and overexpression of SIRT3ct suppressed the toxic function of Aβ in PC12 cells. These results indicate that SIRT3ct promotes cell survival under physiological and pathological conditions.

DOI

臨床病型が大きく異なりFUS遺伝子変異のmosaicismが示唆された家族性ALS母子例

久原 真, 西山 亜由美, 津田 笑子, 鈴木 秀一郎, 松村 晃寛, 石川 亜貴, 櫻井 晃洋, 元池 育子, 青木 正志, 青木 洋子, 下濱 俊

臨床神経学 ( (一社)日本神経学会 )  61 ( Suppl. ) S293 - S293  2021年09月

Sporadic Amyotrophic Lateral Sclerosis Due to a FUS P525L Mutation with Asymmetric Muscle Weakness and Anti-ganglioside Antibodies.

Masanobu Tanemoto, Shin Hisahara, Kazuna Ikeda, Kazuki Yokokawa, Tatsuo Manabe, Reiko Tsuda, Daisuke Yamamoto, Takashi Matsushita, Akihiro Matsumura, Syuuichirou Suzuki, Shun Shimohama

Internal medicine (Tokyo, Japan)   60 ( 12 ) 1949 - 1953  2021年06月  [国内誌]

　概要を見る

Amyotrophic lateral sclerosis (ALS) due to a fused in sarcoma (FUS) P525L mutation is characterized by a rapidly progressive course. Multifocal motor neuropathy (MMN) may resemble ALS in early stage and is associated with anti-ganglioside antibodies. A 38-year-old woman was admitted to our hospital because of progressive muscle weakness in the right limbs. She had mild mental retardation and minor deformities. Initially, we suspected MMN given the asymmetric muscle weakness and detection of anti-ganglioside antibodies. However, physical and electrophysiological tests did not support MMN, instead suggesting ALS. We confirmed a heterozygous P525L mutation and finally diagnosed this case as ALS due to an FUS mutation.

DOI PubMed

SIRT1 decelerates morphological processing of oligodendrocyte cell lines and regulates the expression of cytoskeleton-related oligodendrocyte proteins.

Shin Hisahara, Naotoshi Iwahara, Takashi Matsushita, Syuuichirou Suzuki, Akihiro Matsumura, Mai Fujikura, Kazuki Yokokawa, Taro Saito, Tatsuo Manabe, Jun Kawamata, Yoshiyuki Horio, Shun Shimohama

Biochemical and biophysical research communications   546   7 - 14  2021年03月  [国際誌]

　概要を見る

SIRT1 is involved in the regulation of a variety of biological processes such as metabolism, stress response, autophagy and differentiation. Although progenitor cells of oligodendrocytes (OPCs) express high level of SIRT1, its function on differentiation is unknown. Because we have shown that SIRT1 plays a pivotal role in differentiation of neural precursor cells, we hypothesized that SIRT1 may also participate in the differentiation of oligodendrocytes (OLGs). We examined whether SIRT1 was expressed in two human oligodendrocyte cell lines: KG-1-C and MO 3.13 OLG. Transfection of cell lines with SIRT1-siRNA and SIRT2-siRNA promoted the extension of cellular processes. SIRT1-siRNA and SIRT2-siRNA increased acetyl-α-tubulin level, conversely, over expression of SIRTs resulted in decreased the ratio of acetyl-α-tubulin to α-tubulin. We also found knockdown of SIRT1 and SIRT2 induced overexpression of βIV-tubulin and tubulin polymerization promoting protein (TPPP) (OLG-specific cytoskeleton-related molecules) that distributed widely in cell bodies. Taken together, SIRT1 may play a role in oligodenroglial differentiation and myelinogenesis.

DOI PubMed

Possible Somatic Mosaicism of Novel FUS Variant in Familial Amyotrophic Lateral Sclerosis.

Shin Hisahara, Ayumi Nishiyama, Emiko Tsuda, Syuuichirou Suzuki, Akihiro Matsumura, Aki Ishikawa, Akihiro Sakurai, Ikuko N Motoike, Masashi Aoki, Yoko Aoki, Shun Shimohama

Neurology. Genetics   7 ( 1 ) e552  2021年02月  [国際誌]

DOI PubMed

異なる臨床病型を呈し母にFUS遺伝子変異のmosaicismが示唆された家族性ALSの親子例

久原 真, 津田 笑子, 鈴木 秀一郎, 松村 晃寛, 石川 亜貴, 櫻井 晃洋, 西山 亜由美, 元池 育子, 青木 正志, 青木 洋子, 下濱 俊

臨床神経学 ( (一社)日本神経学会 )  61 ( 1 ) 55 - 55  2021年01月

非典型的な臨床型のFUS遺伝子変異による孤発性と考えられるALSの1例

種本 真将, 眞部 建郎, 横川 和樹, 池田 和奈, 山本 大輔, 松村 晃寛, 鈴木 秀一郎, 久原 真, 松下 隆司, 下濱 俊

臨床神経学 ( (一社)日本神経学会 )  61 ( 1 ) 55 - 55  2021年01月