Misc - 鈴木　健史

Molecular mechanisms and drug development in aquaporin water channel diseases: Water channel aquaporin-2 of kidney collecting duct cells

Kuniaki Takata, Yuki Tajika, Toshiyuki Matsuzaki, Takeo Aoki, Takeshi Suzuki, Ablimit Abduxukur, Haruo Hagiwara

Journal of Pharmacological Sciences   96 ( 3 ) 255 - 259  2004年11月

書評論文，書評，文献紹介等  

　概要を見る

Aquaporin-2 (AQP2) is one of the membrane water channel proteins expressed in principal cells of the kidney collecting ducts. In the basal state, AQP2 resides in the storage vesicles localized in the subapical cytoplasm. Upon stimulation with vasopressin, AQP2 is translocated to the apical plasma membrane by the exocytic fusion of the storage vesicles with the apical membrane. This translocation enables the transepithelial reabsorption of water from the lumen to the interstitium via AQP2 at the apical membrane and AQP3/AQP4 at the basolateral membrane. AQP2-storage vesicles are distinct from the endoplasmic reticulum, Golgi apparatus, trans-Golgi network, and lysosomes. The early endosomal marker EEA1 is colocalized with some of AQP2 vesicles. Further analyses in Madin-Darby canine kidney (MDCK) cells transfected with AQP2 revealed that subapical Rab11-positive/EEA1-negative smaller vesicles constitute part of the AQP2 storage vesicles for the translocation to the apical membrane. Termination of stimulation results in the retrieval of AQP2 to the larger EEA1-positive early endosomal compartment. AQP2 is then transferred to the subapical storage compartment in a PI3-kinase-dependent manner. GLUT4 is an isoform of glucose transporters whose localization is also regulated by vesicular trafficking induced by insulin stimulation. Comparison of the intracellular localization of AQP2 with GLUT4 suggests distinct regulation of AQP2 trafficking.

DOI PubMed CiNii

Aquaporin-2 is retrieved to the apical storage compartment via early endosomes and phosphatidylinositol 3-kinase-dependent pathway

Y Tajika, T Matsuzaki, T Suzuki, T Aoki, H Hagiwara, M Kuwahara, S Sasaki, K Takata

ENDOCRINOLOGY ( ENDOCRINE SOC )  145 ( 9 ) 4375 - 4383  2004年09月

　概要を見る

Aquaporin-2 (AQP2) is one of the water-channel proteins expressed in principal cells of kidney collecting ducts, where it is stored in the intracellular compartment. Previous studies have demonstrated that AQP2 vesicles constitute a distinct intracellular compartment partially overlapping with early endosomes. In this report, we performed in vitro experiments using the renal epithelia] cell line, Madin-Darby canine,kidney (MDCK) cells, stably expressing AQP2 (MDCK-hAQP2). In nonpolarized cells, AQP2 vesicles were scattered in the cytoplasm and did not colocalize with Golgi 58K or TGN38. Small portions of AQP2 vesicles were positive for the lysosome marker cathepsin D. An early endosome antigen (EEA1) localized around AQP2 vesicles in close proximity, suggesting involvement of the endosomal system in the trafficking of AQP2. AQP2 vesicles are distinct from other recycling molecules, such as glucose transporter 4 (GLUT4) and endocytosed transferrin. In polarized MDCK-hAQP2 cells, AQP2 vesicles were localized in the subapical recycling compartment and distinct from the Golgi apparatus, trans-Golgi network, lysosome, and early endosome in the nonstimulated. state. When the cells were treated with forskolin, translocation of AQP2 to the apical membrane was observed. Washout of forskolin induced retrieval of AQP2 into the cytoplasm, and AQP2 was transiently colocalized with EEA1-positive endosomes. Then,. AQP2 moved from EEA1-positive endosomes to the subapical AQP2-storage compartment, which is sensitive to wortmannin and LY294002. These results suggest that AQP2 resides in a recycling compartment at the apical side in polarized MDCK-hAQP2 cells, and its retrieval uses the apical endosomal system and the phosphatidylinositol 3-kinase-dependent pathway.

DOI

ラット乳腺における水チャネルAQP1およびAQP3の局在

松崎利行, 多鹿友喜, ABLIMIT A, 鈴木健史, 青木武生, 萩原治夫, 高田邦昭

日本組織細胞化学会総会・学術集会講演プログラム・予稿集   45th  2004年

J-GLOBAL

29-II-06 Localization and trafficking of aquaporin-2 (AQP2) in the renal epithelial cell(THE JOINT MEETING OF THE 44TH ANNUAL MEETING OF THE JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY AND THE 35TH ANNUAL MEETING OF THE CLINICAL ELECTRON MICROSCOPY SOCIETY OF JAPAN) :

Tajika Yuki, Matsuzaki Toshiyuki, Suzuki Takeshi, Aoki Takeo, Hagiwara Haruo, Takata Kuniaki

Acta histochemica et cytochemica ( Japan Society of Histochemistry and Cytochemistry )  37 ( 1 ) 48 - 48  2004年

CiNii

免疫組織化学 : どの方法を選ぶか(II.免疫組織化学 : 基礎から応用まで)

高田 邦昭, 多鹿 友喜, 松崎 利行, 鈴木 健史, 青木 武生, 萩原 治夫

組織細胞化学 ( 日本組織細胞化学会 )  2003   17 - 24  2003年07月

CiNii

Immunolocalization of water channel, aquaporin-5 (AQP5) in the rat digestive system.

MATSUZAKI TOSHIYUKI, TAJIKA YUKI, SUZUKI TAKESHI, AOKI TAKEO, HAGIWARA HARUO, TAKATA KUNIAKI

Arch Histol Cytol   66 ( 4 ) 307 - 315  2003年

DOI PubMed CiNii

Immunolocalization of water channel, aquaporin-5 (AQP5) in the rat digestive system.

MATSUZAKI TOSHIYUKI, TAJIKA YUKI, SUZUKI TAKESHI, AOKI TAKEO, HAGIWARA HARUO, TAKATA KUNIAKI

Arch Histol Cytol   66 ( 4 ) 307 - 315  2003年

DOI PubMed CiNii

A simple electroporation method for the introduction of plasmids into cells cultured on coverslips for histochemical examination

K Takata, Y Takahashi, T Matsuzaki, Y Tajika, T Suzuki, T Aoki, H Hagiwara

ACTA HISTOCHEMICA ET CYTOCHEMICA ( JAPAN SOC HISTOCHEMISTRY & CYTOCHEMISTRY )  36 ( 4 ) 317 - 323  2003年

　概要を見る

Expression of genes followed by the examination of the localization of their products by histochemical methods is one of important and powerful approaches to elucidate the role of genes of interest. We show here a simple and versatile electroporation method for the introduction of plasmids into cells cultured on coverslips for histochemical examination. Electroporation apparatus was assembled using a 35-mm plastic dish and aluminum wire. The apparatus was placed over the coverslip and plasmid DNA was applied onto it. Green fluorescent protein (GFP) was used as a reporter of transfection. Under the appropriate settings of voltage, duration, and pulse numbers, successful transfection was easily achieved in 3T3-L1 fibroblasts, HeLa cells, Cos7 cells, and MDCK cells. The electroporation method described here is simple, easy, rapid, and economical, and may be best suited for the screening of many genes for their histochemical examination.

DOI CiNii

Cryosectioning of cultured cells on permeable support

Y Tajika, T Matsuzaki, T Suzuki, T Aoki, H Hagiwara, K Takata

ACTA HISTOCHEMICA ET CYTOCHEMICA ( JAPAN SOC HISTOCHEMISTRY & CYTOCHEMISTRY )  36 ( 2 ) 119 - 122  2003年

　概要を見る

Immunolabeling of cultured cell lines of epithelial origin is often performed to study cell polarity. To explore the localization of cellular molecules in detail, we devised an efficient and convenient method to prepare semithin and ultrathin cryosections of cells cultured on permeable support. Multiple specimens were plied up to obtain many vertical images of cells in a single section. Freezing them in cooled n-hexane resulted in clear and homogeneous frozen specimens, from which semithin and ultrathin cryosections of epitherial cells on permeable support were easily obtained. With this method, it is possible to prepare many cryosections of good quality for histochemical examination of vertical images of cells.

DOI

Introduction and expression of glucose transporters in pancreatic acinar cells by in vivo electroporation

Y Shinoda, T Matsuzaki, M Yokoo-Sugawara, T Suzuki, T Aoki, H Hagiwara, H Kuwano, K Takata

ACTA HISTOCHEMICA ET CYTOCHEMICA ( JAPAN SOC HISTOCHEMISTRY & CYTOCHEMISTRY )  36 ( 1 ) 77 - 82  2003年

　概要を見る

cDNAs encoding facilitated-diffusion glucose transporters GULT1, GULT3, GULT4, and GULT5 were introduced into the rat pancreas by in-vivo electroporation method, and their expression and localization in pancreatic acinar cells were examined immunohistochemically. GLUT1 was localized at the basolateral membrane, whereas GLUT3 and GLUT5 were at the apical membrane. Restriction of GLUT3 and GLUT5 to the apical membrane makes a marked contrast to their localization to the entire plasma membrane when expressed in hepatocytes in situ. Such differential localization may be due to differential apical targeting mechanism: direct targeting in acinar cells and indirect transcytotic delivery in hepatocytes in situ. GLUT4 was present in the membrane of zymogen secretory granules in the cytoplasm in acinar cells. This observation suggests that GLUT4 is segregated to the regulated secretory pathway. Expression of glucose transporters in situ is a useful method in analyzing the targeting mechanism of membrane proteins in cells of tissues in situ.

DOI CiNii

A simple electroporation method for the introduction of plasmids into cells cultured on coverslips for histochemical examination.

TAKATA KUNIAKI, TAJIKA TAKAHASHI YUKIKO, MATSUZAKI TOSHIYUKI, TAJIKA YUKI, SUZUKI TAKESHI, AOKI TAKEO, HAGIWARA HARUO

Acta Histochem Cytochem   36 ( 4 ) 317 - 323  2003年

DOI CiNii

カベオラの内在化と膜動態

青木武生, 萩原治夫, 松崎利行, 鈴木健史, 多鹿友喜, 高田邦昭

解剖学雑誌   78 ( Supplement )  2003年

J-GLOBAL

蛍光抗体法 : 基礎から多重染色まで

青木 武生, 松崎 利行, 鈴木 健史, 萩原 治夫, 多鹿 友喜, 高田 邦昭

組織細胞化学 ( 日本組織細胞化学会 )  2002   33 - 41  2002年07月

CiNii

免疫電顕法の実際 : どんな時にどの方法を使うか

萩原 治夫, 青木 武生, 鈴木 健史, 松崎 利行, 高田 邦昭

組織細胞化学 ( 日本組織細胞化学会 )  2002   53 - 64  2002年07月

CiNii

Aquaporins: a water channel family.

MATSUZAKI TOSHIYUKI, TAJIKA YUKI, Nomingerel Tserentsoodol, SUZUKI TAKESHI, AOKI TAKEO, HAGIWARA HARUO, TAKATA KUNIAKI

Anat Sci Int   77 ( 2 ) 85 - 93  2002年

DOI

Aquaporins: a water channel family.

MATSUZAKI TOSHIYUKI, TAJIKA YUKI, Nomingerel Tserentsoodol, SUZUKI TAKESHI, AOKI TAKEO, HAGIWARA HARUO, TAKATA KUNIAKI

Anat Sci Int   77 ( 2 ) 85 - 93  2002年

DOI

多細胞化へのみち,細胞接着の細胞生物学

鈴木 健史, 高田 邦昭

黒岩常祥，中野明彦，財団法人遺伝学普及会偏，細胞のミクロコスモス－進化とゲノムからその素顔にせまる－，裳華房，東京   pp 157-165  2002年

Immunohistochemical characterization of the intracellular pool of water channel aquaporin-2 in the rat kidney.

TAJIKA YUKI, MATSUZAKI TOSHIYUKI, SUZUKI TAKESHI, AOKI TAKEO, HAGIWARA HARUO, Shigeyasu Tanaka, Eiki Kominami, TAKATA KUNIAKI

Anat Sci Int   77 ( 3 ) 189  2002年

DOI

Immunohistochemical characterization of the intracellular pool of water channel aquaporin-2 in the rat kidney.

TAJIKA YUKI, MATSUZAKI TOSHIYUKI, SUZUKI TAKESHI, AOKI TAKEO, HAGIWARA HARUO, Shigeyasu Tanaka, Eiki Kominami, TAKATA KUNIAKI

Anat Sci Int   77 ( 3 ) 189  2002年

DOI

Activation of organelle DNA synthesis during the initial phase of proliferation of BY-2 cultured tobacco cells after medium renewal.

Saori Okamura, SUZUKI TAKESHI, Yutaka Miyazawa, Tsuneyoshi Kuroiwa, Atsushi Sakai

Plant Morphology   14,pp 16-28  2002年