Misc - 鈴木　健史

Direct gene transfer into rat liver cells by in vivo electroporation.

SUZUKI TAKESHI, Shin B-C, Fujikura K, MATSUZAKI TOSHIYUKI, TAKATA KUNIAKI

FEBS Lett   425 ( 3 ) 436  1998年

DOI PubMed CiNii

Direct gene transfer into rat liver cells by in vivo electroporation.

SUZUKI TAKESHI, Bo-Chul Shin, Keiko Fujikura, MATSUZAKI TOSHIYUKI, TAKATA KUNIAKI

FEBS Letters   425 ( 3 ) 436  1998年

DOI PubMed CiNii

ラット胎盤の糖輸送機構．

高田 邦昭, Bo-Chul Shin, 藤倉 恵子, 鈴木 雅一, 鈴木 健史

Mebio   15(No.7),pp 95-97  1998年

上皮細胞における細胞膜糖輸送体の局在機構．

高田 邦昭, 鈴木 健史, 飯塚 昌子, 藤倉 恵子, 松崎 利行, 小山 晴子, 犬飼 浩一, 浅野 知一郎

電子顕微鏡   33 supplement 2,pp 157-160  1998年

乳腺の糖輸送体．

高田 邦昭, 藤倉 恵子, 鈴木 雅一, 鈴木 健史

Mebio   15(No.5),pp 91-93  1998年

ラット胎盤の糖輸送機構

高田 邦昭, Shin B-C, 藤倉 恵子, 鈴木 健史, 松崎 利行

Mebio   15(7),pp95-97  1998年

上皮細胞における細胞膜糖輸送体の局在機構

高田 邦昭, 鈴木 健史, 飯塚 晶子, 藤倉 恵子, 松崎 利行, 小山 晴子, 犬飼 浩一, 浅野 知一郎

電子顕微鏡   33 (suppleemnt 2),pp157-160  1998年

Fluorescence counter-staining of cell nuclear DNA for multi-color laser confocal microscopy

SUZUKI TAKESHI, MATSUZAKI TOSHIYUKI, TAKATA KUNIAKI

Acta Histochem Cytochem   31 ( 4 ) 297 - 301  1998年

DOI CiNii

luorescence counter-staining of cell nuclear DNA for multi-color laser confocal microscopy.

SUZUKI TAKESHI, MATSUZAKI TOSHIYUKI, TAKATA KUNIAKI

Acta Histochem Cytochem   31 ( 4 ) 297 - 301  1998年

DOI CiNii

Fluorescence counter-staining of cell nuclear DNA for multi-color laser confocal microscopy

T Suzuki, T Matsuzaki, K Takata

ACTA HISTOCHEMICA ET CYTOCHEMICA ( JAPAN SOC HISTOCHEMISTRY & CYTOCHEMISTRY )  31 ( 4 ) 297 - 301  1998年

　概要を見る

Twenty-one nucleic acid-specific fluorochromes were examined for use in cell nuclear counter-staining of specimens observed under a laser confocal microscope equipped with a Krypton/argon laser tube. Six green, three red, and two far-red fluorochromes gave sufficient fluorescence. Among them, SYBR Green I, Pico Green, SYTOX Green, YO-PRO-3, and TO-PRO-3 specifically stained the cell nuclear DNA. Other fluorochromes except POPO-3 stained the cell nuclear DNA and cc-stained the cytoplasmic RNA. Conversely, POPO-3 specifically stained the cytoplasmic and nucleolar RNA. Anti-bleaching reagents (PPDA and DABCO) effectively prevented the bleaching of SYBR Green I, Pico Green, YO-PRO-8, and TO-PRO-3, whereas DABCO accelerated the photobleaching of SYTOX Green. Taking into account the characteristics of these fluorochromes, SYBR Green I, YO-PRO-3, and TO-PRO-3 were best suited for cell nuclear counter-staining as green, red, and far-red fluorochromes, respectively. Especially, TO-PRO-3 seems to be the most useful because its far-red fluorescence enables three-color fluorescence staining by combination with immunofluorescence labeling with fluorescein and rhodamine derivatives.

DOI CiNii

Fluorescence counter-staining of cell nuclear DNA for multi-color laser confocal microscopy

SUZUKI TAKESHI, MATSUZAKI TOSHIYUKI, TAKATA KUNIAKI

Acta Histochem Cytochem   31 ( 4 ) 297 - 301  1998年

DOI CiNii

luorescence counter-staining of cell nuclear DNA for multi-color laser confocal microscopy.

SUZUKI TAKESHI, MATSUZAKI TOSHIYUKI, TAKATA KUNIAKI

Acta Histochem Cytochem   31 ( 4 ) 297 - 301  1998年

DOI CiNii

Multiple isoforms of the regulatory subunit for phosphatidylinositol 3-kinase(PI3-kinase)are expressed in neurons in the rat brain.

Shin B-C, SUZUKI TAKESHI, Inukai K, Anai M, Asano T, TAKATA KUNIAKI

Biochem Biophys Res Commun   246 ( 2 ) 313 - 319  1998年

DOI PubMed CiNii

糖輸送体とコネキシン 二層の上皮での経上皮輸送への関与

高田邦昭, SHIN B-C, 藤倉恵子, 鈴木健史, 松崎利行, 田中滋康

日本動物学会大会予稿集   69th  1998年

J-GLOBAL

上皮細胞における細胞膜糖輸送体の局在機構 (日本電子顕微鏡学会第43回シンポジウム論文集--21世紀へ向けての新技術の展開--平成10年10月28日(水)〜30日(金),千葉大学けやき会館〔含 著者名索引〕) -- (DNAの強制発現とそのイメ-ジング)

高田 邦昭, 鈴木 健史, 飯塚 晶子

電子顕微鏡 ( 日本顕微鏡学会 )  33 ( 2 ) 157 - 160  1998年

CiNii

Subcellular distribution of GLUT4 in Chinese hamster ovary cells overexpressing mutant dynamin: Evidence that dynamin is a regulatory GTPase in GLUT4 endocytosis

Waka Omata, Hiroshi Shibata, Yoichi Suzuki, Shigeyasu Tanaka, Takeshi Suzuki, Kuniaki Takata, Itaru Kojima

Biochemical and Biophysical Research Communications ( Academic Press Inc. )  241 ( 2 ) 401 - 406  1997年12月

　概要を見る

We have investigated the subcellular distribution of GLUT4 by immunofluorescence microscopy after transfection with wild-type or mutant dynamin cDNA into Chinese hamster ovary cells expressing insulin receptor and GLUT4. In the basal state, GLUT4 was distributed exclusively within the cells in the cells overexpressing wild-type dynamin (CHO(IR-GLUT4)-WT) but was located at the cell surface in the cells overexpressing mutant dynamin (CHO(IR-GLUT4)-K44E). Insulin induced subcellular shift of GLUT4 to the cell surface in CHO(IR-GLUT4)-WT cells but had little effect in CHO(IR-GLUT4)-K44E cells. When insulin-stimulated cells were treated with wortmannin, GLUT4 was redistributed within the cells in CHO(IR-GLUT4)-WT cell, whereas it remained at the cell surface in CHO(IR-GLUT4)-K44E cell. These results suggest that dynamin is a regulatory GTPase in endocytosis of GLUT4.

DOI PubMed CiNii

Nuclear staining for laser confocal microscopy

T Matsuzaki, T Suzuki, K Fujikura, K Takata

ACTA HISTOCHEMICA ET CYTOCHEMICA ( JAPAN SOC HISTOCHEMISTRY & CYTOCHEMISTRY )  30 ( 3 ) 309 - 314  1997年

　概要を見る

Twenty nucleic acid-specific fluorescent dyes were screened for use in nuclear staining in laser confocal microscopy. Eleven of them, BO-PRO-1, YO-PRO-1, SYBR Green I, PicoGreen, SYTOX Green, TO-PRO-1, POPO-3, propidium iodide, YO-PRO-3, TOTO-3, and TO-PRO-3 gave sufficient fluorescence by in-gel assay. They were also tested for the nuclear staining in cultured cells. When glycerol was used as a mounting medium, it enhanced the fluorescence intensity and retarded the bleaching in most of nucleic acid staining dyes. Among green fluorochromes excited at 488 nm, YO-PRO-1, SYTOX Green, and SYBR Green I were suitable for nuclear DNA staining. YO-PRO-1 showed the strongest fluorescent intensity, but it weakly co-stained the cytoplasmic and nucleolar RNAs. SYTOX Green strongly and specifically stained the nuclear DNA, Among red fluorochromes excited at 568 nm, propidium iodide stained nuclear DNA with simultaneous staining of RNA. YO-PRO-3 strongly and specifically stained DNA but bleached relatively rapidly. TO-PRO-3 excited at 647 nm was specific for DNA, but its fluorescence was bleached rapidly. Selection of nuclear DNA staining dyes should be done by considering the fluorescence intensity, DNA specificity, and bleaching characteristics.

DOI CiNii

Subcellular distributiion of GLUT4 in Chinese hamster ovary cells overexpressing mutant dynamin : Evidence that dynamin in a regulatory GTPase in GLUT4 endocytosis.

Omata W, Shibata H, Suzuki Y, Tanaka S, SUZUKI TAKESHI, TAKATA KUNIAKI, KOJIMA ITARU

Biochem Biophys Res Commun   241 ( 2 ) 401 - 406  1997年

DOI PubMed CiNii

Kir6.1 : a possible subunit of ATP-sensitive K+ channels in mitochondria.

Suzuki M, Kotake K, Fujikura K, Inagaki N, SUZUKI TAKESHI, Gonoi T, Seino S, TAKATA KUNIAKI

Biochem Biophys Res Commun   241 ( 3 ) 693 - 697  1997年

DOI PubMed CiNii

Kir6.1 : a possible subunit of ATP-sensitive K+ channels in mitochondria.

Suzuki M, Kotake K, Fujikura K, Inagaki N, SUZUKI TAKESHI, Gonoi T, Seino S, TAKATA KUNIAKI

Biochem Biophys Res Commun   241 ( 3 ) 693 - 697  1997年

DOI PubMed CiNii