Misc - 鈴木　健史

Immunolocalization of tight junction proteins, occludin and ZO-1, and glucose transporter GLUT1 in the cells of the blood-nerve barrier

TAKATA KUNIAKI, SUZUKI TAKESHI, Tserentsoodol N, Shin B-C, Koyama H

Arch Histol Cytol   62 ( 5 ) 459 - 469  1999年

DOI PubMed CiNii

Peptide transporter (PepT1) in the rat small intestine : ultrastructural localization and effect of starvation and administration of amino acids.

Ogihara T, SUZUKI TAKESHI, Nagamachi Y, Inui K, TAKATA KUNIAKI

Histochem J   31,pp169-174  1999年

Sugar transporters in polarized epithelial cells

Kuniaki Takata, Akiko Iizuka, Takeshi Suzuki, Toshiyuki Matsuzaki, Minako Sugawara, Bo-Chul Shin, Tserentsoodol Nomingerel, Yasuo Shinoda

Acta Histochemica et Cytochemica ( Japan Society of Histochemistry and Cytochemistry )  32 ( 1 ) 53 - 58  1999年

　概要を見る

Sugar transporters are integral membrane proteins responsible for the transport of sugars across the cellular membranes. They play important roles in the transfer of sugars across different compartments separated by epithelia in the body. Preferential localization of them in specific plasma membrane domains of epithelial cells is crucial in the vectorial transfer of sugars. Modification of the transporter genes with the molecular biology techniques and visualization of the expressed gene products by immunohistochemical methods have shed light on the molecular mechanism underlying the specific localization of the transporter molecules.

DOI

Sugar transporters in polarized epithelial cells.

TAKATA KUNIAKI, Akiko Iizuka, SUZUKI TAKESHI, MATSUZAKI TOSHIYUKI, YOKOO MINAKO, Bo-Chul Shin, Tserentsoodol Nomingerel, Yasuo Shinoda

Acta Histochem Cytochem   32 ( 1 ) 53 - 58  1999年

DOI

Sugar transporters in polarized epithelial cells

K Takata, A Iizuka, T Suzuki, T Matsuzaki, M Sugawara, BC Shin, T Nomingerel, Y Shinoda

ACTA HISTOCHEMICA ET CYTOCHEMICA ( JAPAN SOC HISTOCHEMISTRY & CYTOCHEMISTRY )  32 ( 1 ) 53 - 58  1999年

　概要を見る

Sugar transporters are integral membrane proteins responsible for the transport of sugars across the cellular membranes. They play important roles in the transfer of sugars across different compartments separated by epithelia in the body. Preferential localization of them in specific plasma membrane domains of epithelial cells is crucial in the vectorial transfer of sugars. Modification of the transporter genes with the molecular biology techniques and visualization of the expressed gene products by immunohistochemical methods have shed light on the molecular mechanism underlying the specific localization of the transporter molecules.

DOI

Immunolocalization and effect of secretory stimulation

MATSUZAKI TOSHIYUKI, SUZUKI TAKESHI, TAKATA KUNIAKI, Koyama H, Tanaka S

Cell Tissue Res   295,pp513-521  1999年

DOI

Aquaporin-5 (AQP5), a water channel protein, in the rat salivary and lacrimal gland : Immunolocalization and effect of secretory stimulation.

MATSUZAKI TOSHIYUKI, SUZUKI TAKESHI, Koyama H, Tanaka S, TAKATA KUNIAKI

Cell Tissue Res   295,pp513-521  1999年

DOI

Aquaporin-5(AQP5), a water channel protein, in the rat salivary and lacrimal glands : immunolocalization and effect of secretory stimulation

松崎 利行, 鈴木 健史, 高田 邦昭, 小山晴子, 田中滋康

Cell & Tissue Research   295/,513-521  1999年

DOI

Aquaporin-5 (AQP5), a water channel protein, in the rat salivary and lacrimal gland : Immunolocalization and effect of secretory stimulation.

MATSUZAKI TOSHIYUKI, SUZUKI TAKESHI, Koyama H, Tanaka S, TAKATA KUNIAKI

Cell Tissue Res   295,pp513-521  1999年

DOI

Immunolocalization and effect of secretory stimulation

MATSUZAKI TOSHIYUKI, SUZUKI TAKESHI, TAKATA KUNIAKI, Koyama H, Tanaka S

Cell Tissue Res   295,pp513-521  1999年

DOI

Peptide transporter (PepT1) in the rat small intestine : ultrastructural localization and effect of starvation and administration of amino acids.

Ogihara T, SUZUKI TAKESHI, Nagamachi Y, Inui K, TAKATA KUNIAKI

Histochem J   31,pp169-174  1999年

Immunolocalization of water channel protein, aquaporin 3, in the rat urinary system :

MATSUZAKI Toshiyuki, SUZUKI Takeshi, TAKATA Kuniaki

Acta histochemica et cytochemica ( Japan Society of Histochemistry and Cytochemistry )  32 ( 6 ) 546 - 546  1999年

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ラット泌尿器系における水チャネル,アクアポリン3の局在

松崎利行, 鈴木健史, 高田邦昭

日本組織細胞化学会総会・学術集会講演プログラム・予稿集   40th  1999年

J-GLOBAL

ラット上皮組織における水チャネル,アクアポリン3

松崎利行, 鈴木健史, 小山晴子, 田中滋康, 高田邦昭

日本細胞生物学会大会講演要旨集   52nd  1999年

J-GLOBAL

S VIII-1 細胞膜の糖輸送体SGLT1の局在機構

鈴木 健史, 高田 邦昭

日本組織細胞化学会総会プログラムおよび抄録集 ( 日本組織細胞化学会 )  ( 40 ) 91 - 91  1999年

CiNii

Plasma membrane localizization mechanism of the glucose transporter SGLT1 :

Suzuki Takeshi, Takata Kuniaki

Acta histochemica et cytochemica ( Japan Society of Histochemistry and Cytochemistry )  32 ( 6 ) 504 - 504  1999年

CiNii

Multiple isoforms of the regulatory subunit for phosphatidylinositol 3-kinase (PI3-kinase) are expressed in neurons in the rat brain

Bo-Chul Shin, Masakazu Suzuki, Kouichi Inukai, Motonobu Anai, Tomoichiro Asano, Kuniaki Takata

Biochemical and Biophysical Research Communications ( Academic Press Inc. )  246 ( 2 ) 313 - 319  1998年05月

　概要を見る

Phosphatidylinositol 3-kinase (PI3-kinase) is a heterodimeric enzyme composed of a catalytic subunit of 110 kDa and an adaptor regulatory subunit. We investigated the presence and localization of five isoforms of the regulatory subunits, p55α, p55γ, p85α, p85β, and p50α, in the rat brain. In situ hybridization histochemistry using isoform-specific cRNA probes revealed that all five isoforms were expressed in the neurons of the brain. Interestingly, most neuronal cells including Purkinje cells in the cerebellum and pyramidal cells in the cerebrum expressed all five isoforms. Immuno-histochemical staining also showed the localization of p55α, p55γ, p85α, and p50α in the neuronal cells in the brain. Expression of multiple isoforms in neurons suggests that they may play important roles in signal transduction in the brain.

DOI PubMed CiNii

Direct gene transfer into rat liver cells by in vivo electroporation

Takeshi Suzuki, Bo-Chul Shin, Keiko Fujikura, Toshiyuki Matsuzaki, Kuniaki Takata

FEBS Letters   425 ( 3 ) 436 - 440  1998年04月

　概要を見る

In vivo electro-transfection efficiency and manner of transferred gene expression were investigated by fluorescence microscopic image analysis. Green fluorescent protein (GFP) gene was used as the genetic marker, Electroporation was carried out on the liver of live rats by use of disk electrodes mounted in the tips of tweezers, which were directly pressed onto the surface of a liver lobe in situ. Electroporation with eight electric pulses of 50 ms in duration at 50 V gave a good efficiency of transfection as judged by the induced GFP expression. Bright fluorescence of GFP appeared as dots, which were scattered around the area damaged by electroporation. The transfection efficiency increased as the amount of injected DNA was increased. The results indicate that the amount of induced gene expression can be controlled. Estimation of the efficiency of electro-gene transfer using the fluorescence of GFP and digital analysis of microscopic images was useful to determine the optimum conditions for local gene therapy in tissues and organs.

DOI PubMed CiNii

Multiple isoforms of the regulatory subunit for phosphatidylinositol 3-kinase (PI3-kinase) are expressed in neurons in the rat brain.

Shin B-C, SUZUKI TAKESHI, Inukai K, Anai M, Asano T, TAKATA KUNIAKI

Biochem Biophys Res Commun   246 ( 2 ) 313 - 319  1998年

DOI PubMed CiNii

Multiple isoforms of the regulatory subunit for phosphatidylinositol 3-kinase(PI3-kinase)are expressed in neurons in the rat brain.

Shin B-C, SUZUKI TAKESHI, Inukai K, Anai M, Asano T, TAKATA KUNIAKI

Biochem Biophys Res Commun   246 ( 2 ) 313 - 319  1998年

DOI PubMed CiNii