Misc - SUZUKI Takeshi

Immunohistochemical characterization of the intracellular pool of water channel aquaporin-2 in the rat kidney.

TAJIKA YUKI, MATSUZAKI TOSHIYUKI, SUZUKI TAKESHI, AOKI TAKEO, HAGIWARA HARUO, Shigeyasu Tanaka, Eiki Kominami, TAKATA KUNIAKI

Anat Sci Int   77 ( 3 ) 189  2002

DOI

多細胞化へのみち--細胞接着の細胞生物学 (細胞のミクロコスモス--進化とゲノムからその素顔にせまる)

鈴木 健史, 高田 邦昭

生物の科学「遺伝」別冊 ( 裳華房 )  ( 14 ) 157 - 165  2002

CiNii

A close relationship between EEA1 and internalized caveolae structure :

Aoki Takeo, Hagiwara Haruo, Suzuki Takeshi, Matsuzaki Toshiyuki, Takata Kuniaiki

Acta histochemica et cytochemica ( Japan Society of Histochemistry and Cytochemistry )  35 ( 1 ) 59 - 59  2002

　View Summary

Internalization of caveolae structure on the cell surface is promoted by oxidant stress or inhibitor of phosphatase (Aoki et al., Exp Cell Res 253, 629, 1999). The internalization is accompanied by phosphorylation of caveolin-1 , a marker protein of caveolae. Little is known about the significance of this internalization. Recently we showed that phosphorylated caveolin-1 forms a complex with Rab5 protein. The existence of Rab5-caveolin-1 complex suggests that internalized caveolac could be translocated to endosomes. To see whether early endosome marker protein EEA1 is related to caveolae, we examined internalized caveolae by immunofluorescence microscopy, cryoultrathin immunogold electron microscopy, immunoprecipitation. Immunofluorescence examination revealed that internalized caveolae colocalized with EEA1 in many area. By immunoprecipitation, both caveolin-1 antibody and phosphorylated caveolin-1 antibody precipitated EEA1 protein. When caveolae were internalized, EEA1 antibody effectively precipitated many phosphorylated caveolin-1 . On ultrathin cryosections of cultured endothelial cells and caveolae-rich fractions obtained by sucrose density-centrifugatioin method, we confirmed that EEA1 colocalized with caveolae structure. These results show a close relationship between EEA1 and caveolae structure.

CiNii

免疫組織化学の基礎的手順 : 抗体入手から検境まで

高田 邦昭, 松崎 利行, 鈴木 健史, 青木 武生, 萩原 治夫, 藤倉 恵子

組織細胞化学 ( 日本組織細胞化学会 )  2001   80 - 87  2001.07

CiNii

The apical localization of SGLT1 glucose transporter is determined by the short amino acid sequence in its Nterminal domain.

SUZUKI TAKESHI, Keiko Fujikura, Haruko Koyama, MATSUZAKI TOSHIYUKI, TAJIKA TAKAHASHI YUKIKO, TAKATA KUNIAKI

Eur J Cell Biol   80,pp 765-774  2001

Expression of sugar transporters by in vitro electroporation and particle gun methods in the rat liver: localization to specific membrane domains.

Yasuo Shinoda, SUZUKI TAKESHI, YOKOO MINAKO, Shinya Nagamatsu, KUWANO HIROYUKI, TAKATA KUNIAKI

Acta Histochem Cytochem   34 ( 1 ) 15 - 24  2001

DOI CiNii

Expression of sugar transporters by in vitro electroporation and particle gun methods in the rat liver: localization to specific membrane domains.

Yasuo Shinoda, SUZUKI TAKESHI, YOKOO MINAKO, Shinya Nagamatsu, KUWANO HIROYUKI, TAKATA KUNIAKI

Acta Histochem Cytochem   34 ( 1 ) 15 - 24  2001

DOI CiNii

Glucose transporters - Localization to specific membrane domains.

TAKATA KUNIAKI, SUZUKI TAKESHI, MATSUZAKI TOSHIYUKI, Nomingerel Tserentsoodol, TAJIKA YUKI, Yasuo Shinoda, HAGIWARA HARUO, AOKI TAKEO

電子顕微鏡   36 supplement 2,pp 23-25  2001

Hypertonicity-induced expression of aquaporin 3 in MDCK cells.

MATSUZAKI TOSHIYUKI, SUZUKI TAKESHI, TAKATA KUNIAKI

Am J Physiol   281,pp C55-C63  2001

The apical localization of SGLT1 glucose transporter is determined by the short amino acid sequence in its Nterminal domain.

SUZUKI TAKESHI, Keiko Fujikura, Haruko Koyama, MATSUZAKI TOSHIYUKI, TAJIKA TAKAHASHI YUKIKO, TAKATA KUNIAKI

Eur J Cell Biol   80,pp 765-774  2001

Glucose transporters - Localization to specific membrane domains.

TAKATA KUNIAKI, SUZUKI TAKESHI, MATSUZAKI TOSHIYUKI, Nomingerel Tserentsoodol, TAJIKA YUKI, Yasuo Shinoda, HAGIWARA HARUO, AOKI TAKEO

電子顕微鏡   36 supplement 2,pp 23-25  2001

Hypertonicity-induced expression of aquaporin 3 in MDCK cells.

MATSUZAKI TOSHIYUKI, SUZUKI TAKESHI, TAKATA KUNIAKI

Am J Physiol   281,pp C55-C63  2001

MDCK細胞における水チャネルタンパク質,アクアポリン3の高浸透圧刺激による発現変化

松崎利行, 鈴木健史, 高田邦昭

解剖学雑誌   76 ( 1 )  2001

J-GLOBAL

初期エンドソーム蛋白質EEA1と内在化カベオラの関連性

青木武生, 萩原治夫, 鈴木健史, 松崎利行, 高田邦昭

日本組織細胞化学会総会・学術集会講演プログラム・予稿集   42nd  2001

J-GLOBAL

S3-4 Intracellular Localization and Dynamics of Insulin-recruitable Glucose Transporter GLUT4 :

TAKATA Kuniaki, FUJIKURA Keiko, SHINODA Yasuko, SUZUKI Takeshi

Acta histochemica et cytochemica ( Japan Society of Histochemistry and Cytochemistry )  34 ( 1 ) 47 - 47  2001

CiNii

B-1 Immunolocalization of Organic Cation Transporters, rOCT1 and rOCT2 in the Rat Kidney :

SUGAWARA-YOKOO Minako, MATSUZAKI Toshiyuki, SUZUKI Takeshi, TAKATA Kuniaki

Acta histochemica et cytochemica ( Japan Society of Histochemistry and Cytochemistry )  34 ( 1 ) 70 - 70  2001

CiNii

Immunohistochemical analysis of intracellular localization of insulin-sensitive glucose transporter GLUT4 in 3T3-L1 adipocytes

FUJIKURA K., SUZUKI M., SUZUKI T., SUGAWARA-YOKOO M., MATSUZAKI T., TAKATA K.

電子顕微鏡   35   465 - 465  2000.05

CiNii

上皮細胞におけるナトリウムイオン依存型糖輸送体SGLT1の頂部細胞膜局在機構．

鈴木 健史

北関東医学 ( The Kitakanto Medical Society )  50,pp 399-401 ( 4 ) 399 - 401  2000

DOI CiNii

An anti-peptide antibody that recognized unexpected protein - A case report

Toshiyuki Matsuzaki, Suzuki Takeshi, Shigeyasu Tanaka, Kuniaki Tanaka

Acta Histochemica et Cytochemica   33 ( 5 ) 361 - 365  2000

　View Summary

Rabbits were immunized with partial peptides corresponding to dog Na +-coupled myoinositol cotransporter (SMIT) and rat water channel aquaporin 3 (AQP3). Immunoblot analysis and immunofluorescence microscopy revealed that the antibodies corresponding to SMIT and to AQP3 both detected AQP3 protein in the kidney. Neither of them, on the other hand, detected SMIT protein. Immunogen peptides of both SMIT and AQP3 contain the identical sequence "KEQI", whereas other amino acids are different from each other. Immunohistochemical preabsorption studies demonstrated that the antibodies corresponding to SMIT mainly recognized the sequence "KEQI" as an epitope. These observations demonstrate that the utmost care should be taken in using anti-peptide antibodies.

DOI CiNii

An anti-peptide antibody that recognized unexpected protein---A case report

MATSUZAKI TOSHIYUKI, SUZUKI TAKESHI, Tanaka S, TAKATA KUNIAKI

Acta Histochem Cytochem   33 ( 5 ) 361 - 365  2000

DOI CiNii