Misc - SUZUKI Takeshi

An anti-peptide antibody that recognized unexpected protein - A case report

T Matsuzaki, T Suzuki, S Tanaka, K Takata

ACTA HISTOCHEMICA ET CYTOCHEMICA ( JAPAN SOC HISTOCHEM CYTOCHEM )  33 ( 5 ) 361 - 365  2000

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Rabbits were immunized with partial peptides corresponding to dog Naf-coupled myoinositol cotransporter (SMIT) and rat water channel aquaporin 3 (AQPS). Immunoblot analysis and immunofluorescence microscopy revealed that the antibodies corresponding to SMIT and to AQP3 both detected AQP3 protein in the kidney. Neither of them, on the other hand, detected SMIT protein. Immunogen peptides of both SMIT and AQP3 contain the identical sequence "KEQI" whereas other amino acids are different from each other. Immunohistochemical preabsorption studies demonstrated that the antibodies corresponding to SMIT mainly recognized the sequence "KEQI" as an epitope. These observations demonstrate that the utmost care should be taken in using anti-peptide antibodies.

DOI CiNii

An anti-peptide antibody that recognized unexpected protein - a case report.

MATSUZAKI TOSHIYUKI, SUZUKI TAKESHI, Shigeyasu Tanaka, TAKATA KUNIAKI

Acta Histochem Cytochem   33 ( 5 ) 361 - 365  2000

DOI CiNii

Water channel protein, aquaporin 3, in epithelial cells

MATSUZAKI TOSHIYUKI, SUZUKI TAKESHI, TAKATA KUNIAKI

Kluwer Academic/Plenum Publishers, New York   pp167-172  2000

Water channel protein, aquaporin 3, in epithelial cells

MATSUZAKI TOSHIYUKI, SUZUKI TAKESHI, TAKATA KUNIAKI

Kluwer Academic/Plenum Publishers, New York   pp167-172  2000

ラット上皮組織およびMDCK細胞における水チャネルタンパク質,アクアポリン3

松崎利行, 鈴木健史, 田中滋康, 高田邦昭

解剖学雑誌   75 ( 5 )  2000

J-GLOBAL

糖輸送体SGLT1の発現調節系を用いた頂部細胞膜局在分布機構の解析

鈴木健史, 小山晴子, 松崎利行, 藤倉恵子, 高田邦昭

解剖学雑誌   75 ( 1 )  2000

J-GLOBAL

ラット唾液腺の水チャネル,アクアポリン5

松崎利行, 鈴木健史, 高田邦昭

解剖学雑誌   75 ( 1 )  2000

J-GLOBAL

ラット腎臓における有機カチオントランスポーター(OCT1,OCT2)の分布局在

横尾美奈子, 松崎利行, 鈴木健史, 高田邦昭

日本組織細胞化学会総会・学術集会講演プログラム・予稿集   41st  2000

J-GLOBAL

ラット腎臓における促進拡散型糖輸送体GLUT4の局在

横尾美奈子, 鈴木健史, 松崎利行, 藤倉恵子, 成清卓二, 高田邦昭

解剖学雑誌   75 ( 1 )  2000

J-GLOBAL

糖輸送体SGLT1の頂部細胞膜移送経路の解析

鈴木健史, 小山晴子, 松崎利行, 高田邦昭

日本細胞生物学会大会講演要旨集   53rd  2000

J-GLOBAL

Laser confocal microscopic analysis of the localization of Na^+-dependent glucose transporter SGLT1

SUZUKI T., FUJIKURA K., KOYAMA H., MAGSHZAKI T., TAKATA K.

Electron-microscopy ( 日本電子顕微鏡学会 )  34   105 - 108  1999.11

CiNii

Immunolocalization of tight junction proteins, occludin and ZO-1, and glucose transporter GLUT1 in the cells of the blood-nerve barrier

TAKATA KUNIAKI, SUZUKI TAKESHI, Tserentsoodol N, Shin B-C, Koyama H

Arch Histol Cytol   62 ( 5 ) 459 - 469  1999

DOI PubMed CiNii

Aquaporin-5 (AQP5), a water channel protein, in the rat salivary and lacrimal gland : Immunolocalization and effect of secretory stimulation.

MATSUZAKI TOSHIYUKI, SUZUKI TAKESHI, Koyama H, Tanaka S, TAKATA KUNIAKI

Cell Tissue Res   295,pp513-521  1999

DOI

Immunolocalization and effect of secretory stimulation

MATSUZAKI TOSHIYUKI, SUZUKI TAKESHI, TAKATA KUNIAKI, Koyama H, Tanaka S

Cell Tissue Res   295,pp513-521  1999

DOI

Peptide transporter (PepT1) in the rat small intestine : ultrastructural localization and effect of starvation and administration of amino acids.

Ogihara T, SUZUKI TAKESHI, Nagamachi Y, Inui K, TAKATA KUNIAKI

Histochem J   31,pp169-174  1999

Peptide transporter (PepT1) in the rat small intestine : ultrastructural localization and effect of starvation and administration of amino acids.

Ogihara T, SUZUKI TAKESHI, Nagamachi Y, Inui K, TAKATA KUNIAKI

Histochem J   31,pp169-174  1999

Presence of fructose transporter GLUT5 in the S3 proximal tubules in the rat kidney

Minako Sugawara-Yokoo, Takeshi Suzuki, Toshiyuki Matsuzaki, Takuji Naruse, Kuniaki Takata

Kidney International ( Blackwell Publishing Inc. )  56 ( 3 ) 1022 - 1028  1999

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Background. Fructose is a nutrient as well as a potent agent for the formation of advanced glycation end product in diabetes. GLUT5 is a facilitated-diffusion fructose transporter expressed in the small intestine and kidney. Previous reports on the localization of GLUT5 by in situ hybridization and immunohistochemistry were controversial. Methods. The expression of GLUT5 was checked by reverse transcription-polymerase chain reaction and Southern blotting and immunoblotting analyses. Localization of GLUT5 was visualized by high-resolution immunofluorescence and immunogold electron microscopy. Results. We were able to confirm the expression of GLUT5 in the kidney. GLUT5 was predominantly present in the outer stripe of the outer medulla, where it was localized in the S3 proximal tubule cells. Double labeling with phalloidin showed that GLUT5 was localized in the brush border of the S3 proximal tubule cells. Ultrastructural examination revealed that GLUT5 was present along the plasma membrane of the apical microvilli. Conclusion. GLUT5 is present at the apical plasma membrane of S3 proximal tubule cells and may serve as the transporter of fructose.

DOI PubMed

Presence of fructose transporter GLUT5 in the S3 proximal tubules in the rat

YOKOO MINAKO, SUZUKI TAKESHI, MATSUZAKI TOSHIYUKI, TAKATA KUNIAKI, Naruse T

Kidney Int   56,pp1022-1028  1999

DOI

Presence of fructose transporter GLUT5 in the S3 proximal tubules in the rat

YOKOO MINAKO, SUZUKI TAKESHI, MATSUZAKI TOSHIYUKI, Naruse T, TAKATA KUNIAKI

Kidney Int   56,pp1022-1028  1999

DOI

Peptide transporter in the rat small intestine: Ultrastructural localization and the effect of starvation and administration of amino acids

Hiroshi Ogihara, Takeshi Suzuki, Yukio Nagamachi, Ken-Ichi Inui, Kuniaki Takata

Histochemical Journal   31 ( 3 ) 169 - 174  1999

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Peptide transporter-1 is a H+/peptide cotransporter responsible for the uptake of small peptides and peptide-like drugs, and is present in the absorptive epithelial cells of the villi in the small intestine (duodenum, jejunum, and ileum). It has been localized to the apical microvillous plasma membrane of the absorptive epithelial cells of the rat small intestine using the immunogold electron microscopic technique. Digital image analysis of the jejunum revealed that the transporter protein was abundant at the tip of the villus and that the amount decreased from the tip of the villus to its base. The effect of dietary administration of amino acids and starvation on the expression of PepT1 in the jejunum was examined by immunoblotting and image analysis of immunofluorescence. Starvation markedly increased the amount of peptide transporter present, whereas dietary administration of amino acids reduced it. The gradient of the transporter protein along the crypt-villus axis was maintained under either condition. These observations show that it is specific to the microvillous plasma membrane and that its expression is regulated by the nutritional condition.

DOI PubMed