Misc - SUZUKI Takeshi

Na+-dependent glucose transporter SGLT1 is localized in the apical plasma membrane upon completion of tight junction formation in MDCK cells

Takeshi Suzuki, Keiko Fujikura, Kuniaki Takata

Histochemistry and Cell Biology ( Springer Verlag )  106 ( 6 ) 529 - 533  1996

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SGLT1, an isoform of Na+-dependent glucose transporters, is localized at the apical plasma membrane in the epithelial cells of the small intestine and the kidney. In the present study we examined its location in SGLT1 cDNA-transfected MDCK cells, which form an epithelial sheet connected by tight junctions in culture. Formation of tight junctions was monitored by staining for occludin, an integral tight junction protein. In the cells demarcated by an uninterrupted occluding meshwork, SGLT1 was specifically localized at the apical plasma membrane, showing that SGLT1 has a signal to accomplish this restricted localization. In the cells with little or no occludin accumulation in the tight junction, however, SGLT1 was present along the entire aspect of the plasma membrane. Similar distribution of SGLT1 was observed in the cells as long as the occludin meshwork remained incomplete. These observations suggest that apical localization of SGLT1 occurs upon the completion of the uninterrupted meshwork of tight junctions.

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Na⁺-dependent glucose transporter SGLT1 is localized in the apical plasma membrane upon completion of tight junction formation in MDCK cells.

SUZUKI TAKESHI, Fujikura K, TAKATA KUNIAKI

Histochem Cell Biol   106,pp529-533  1996

DOI

Na+-dependent glucose transporter SGLT1 is localized in the apical plasma membrane upon completion of tight junction formation in MDCK cells.

SUZUKI TAKESHI, Keiko Fujikura, TAKATA KUNIAKI

Histochem Cell Biol   106,pp 529-533  1996

DOI

蛍光抗体法

高田邦昭, Shin Bo-Chul, 鈴木 健史, 藤倉 恵子

組織細胞化学1996 ( 日本組織細胞化学会 )    107 - 113  1996

CiNii

ギャップ結合と糖輸送．

鈴木 健史, 高田 邦昭, 松崎 利行, Bo-Chul Shin

Mebio   12(No.11),pp 149-151  1995

Localization of organelle DNA synthesis within the root apical meristem of rice.

SUZUKI TAKESHI, Narie Sasaki, Atsushi Sakai, Shigeyuki Kawano, Tsuneyoshi Kuroiwa

J Exp Botany   46,pp 19-25  1995

Localization of organelle DNA synthesis within the root apical meristem of rice.

SUZUKI TAKESHI, Narie Sasaki, Atsushi Sakai, Shigeyuki Kawano, Tsuneyoshi Kuroiwa

J Exp Botany   46,pp 19-25  1995

組織細胞内におけるオルガネラDNA合成部位の検出法 -テクノビット-BrdU抗体蛍光抗体染色法-．

鈴木 健史

植物細胞工学   4,pp 416-417  1992

Preferential mitochondrial and plasmid DNA synthesis before multiple cell division in Nicotiana tabacum.

SUZUKI TAKESHI, Shigeyuki Kawano, Atsushi Sakai, Makoto Fujie, Haruko Kuroiwa, Hiromu Nakamura, Tsuneyoshi Kuroiwa

J Cell Sci   103,pp.831-837  1992

オルガネラゲノムからみた細胞の増殖 -細胞周期はあるか-．

鈴木 健史, 黒岩 常祥

植物細胞工学   4,pp 405-415  1992

Preferential mitochondrial and plasmid DNA synthesis before multiple cell division in Nicotiana tabacum.

SUZUKI TAKESHI, Shigeyuki Kawano, Atsushi Sakai, Makoto Fujie, Haruko Kuroiwa, Hiromu Nakamura, Tsuneyoshi Kuroiwa

J Cell Sci   103,pp.831-837  1992

The rice mitochondrial nad3 gene has an extended reading frame at its 5' end: Nucleotide sequence analysis of rice trnS, nad3, and rps12 genes.

SUZUKI TAKESHI, Shigeru Kazama, Atsushi Hirai, Tomoya Akihama, Koh-ichi Kadowaki

Curr Genet   20 ( 4 ) 331 - 337  1991

DOI PubMed CiNii

The rice mitochondrial nad3 gene has an extended reading frame at its 5' end: Nucleotide sequence analysis of rice trnS, nad3, and rps12 genes.

SUZUKI TAKESHI, Shigeru Kazama, Atsushi Hirai, Tomoya Akihama, Koh-ichi Kadowaki

Curr Genet   20 ( 4 ) 331 - 337  1991

DOI PubMed CiNii