Misc - SUZUKI Takeshi

Peptide transporter (PepT1) in the rat small intestine: ultrastructural localization and effect of starvation and administration of amino acids

TAKATA KUNIAKI, SUZUKI TAKESHI, Ogihara T, Nagamachi Y, Inui K

Histochem   J 31,pp169-174  1999

DOI

Water channel protein AQP3 is present in epithelia exposed to the environment of possible water loss

Toshiyuki Matsuzaki, Takeshi Suzuki, Haruko Koyama, Shigeyasu Tanaka, Kuniaki Takata

Journal of Histochemistry and Cytochemistry ( Histochemical Society Inc. )  47 ( 10 ) 1275 - 1286  1999

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Aquaporins (AQPs) are membrane water channel proteins expressed in various tissues in the body. We surveyed the immunolocalization of AQP3, an isoform of the AQP family, in rat epithelial tissues. AQP3 was localized to many epithelial cells in the urinary, digestive, and respiratory tracts and in the skin. In the urinary tract, AQP3 was present at transitional epithelia. In the digestive tract, abundant AQP3 was found in the stratified epithelia in the upper part, from the oral cavity to the forestomach, and in the simple and stratified epithelia in the lower part, from the distal colon to the anal canal. In the respiratory tract, AQP3 was present in the pseudostratified ciliated epithelia from the nasal cavity to the intrapulmonary bronchi. In the skin, AQP3 was present in the epidermis. Interestingly, AQP3 was present at the basal aspects of the epithelia: in the basolateral membranes in the simple epithelia and in the multilayered epithelia at plasma membranes of the basal to intermediate cells. During development of the skin, AQP3 expression commenced late in fetal life. Because these AQP3-positive epithelia have a common feature, i.e., they are exposed to an environment of possible water loss, we propose that AQP3 could serve as a water channel to provide these epithelial cells with water from the subepithelial side to protect them against dehydration.

DOI PubMed CiNii

Water channel protein AQP3 is present in epithelia exposed to the environment of possible water loss

松崎 利行, 鈴木 健史, 高田 邦昭, 小山晴子, 田中滋康

Journal of Histochemistry & Cytochemistry   47/1275-1286  1999

DOI

Water channel protein AQP3 is present in the epithelia exposed to the environment of possible water loss

MATSUZAKI TOSHIYUKI, SUZUKI TAKESHI, Koyama H, Tanaka S, TAKATA KUNIAKI

J Histochem Cytochem   47 ( 10 ) 1275 - 1286  1999

DOI PubMed CiNii

Immunolocalization of tight junction proteins, occludin and ZO-1, and glucose transporter GLUT1 in the cells of the blood-nerve barrier

TAKATA KUNIAKI, SUZUKI TAKESHI, Tserentsoodol N, Shin B-C, Koyama H

Arch Histol Cytol   62 ( 5 ) 459 - 469  1999

DOI PubMed CiNii

Aquaporin-5(AQP5), a water channel protein, in the rat salivary and lacrimal glands : immunolocalization and effect of secretory stimulation

松崎 利行, 鈴木 健史, 高田 邦昭, 小山晴子, 田中滋康

Cell & Tissue Research   295/,513-521  1999

DOI

Sugar transporters in polarized epithelial cells

Kuniaki Takata, Akiko Iizuka, Takeshi Suzuki, Toshiyuki Matsuzaki, Minako Sugawara, Bo-Chul Shin, Tserentsoodol Nomingerel, Yasuo Shinoda

Acta Histochemica et Cytochemica ( Japan Society of Histochemistry and Cytochemistry )  32 ( 1 ) 53 - 58  1999

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Sugar transporters are integral membrane proteins responsible for the transport of sugars across the cellular membranes. They play important roles in the transfer of sugars across different compartments separated by epithelia in the body. Preferential localization of them in specific plasma membrane domains of epithelial cells is crucial in the vectorial transfer of sugars. Modification of the transporter genes with the molecular biology techniques and visualization of the expressed gene products by immunohistochemical methods have shed light on the molecular mechanism underlying the specific localization of the transporter molecules.

DOI

Sugar transporters in polarized epithelial cells.

TAKATA KUNIAKI, Akiko Iizuka, SUZUKI TAKESHI, MATSUZAKI TOSHIYUKI, YOKOO MINAKO, Bo-Chul Shin, Tserentsoodol Nomingerel, Yasuo Shinoda

Acta Histochem Cytochem   32 ( 1 ) 53 - 58  1999

DOI

Sugar transporters in polarized epithelial cells

K Takata, A Iizuka, T Suzuki, T Matsuzaki, M Sugawara, BC Shin, T Nomingerel, Y Shinoda

ACTA HISTOCHEMICA ET CYTOCHEMICA ( JAPAN SOC HISTOCHEMISTRY & CYTOCHEMISTRY )  32 ( 1 ) 53 - 58  1999

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Sugar transporters are integral membrane proteins responsible for the transport of sugars across the cellular membranes. They play important roles in the transfer of sugars across different compartments separated by epithelia in the body. Preferential localization of them in specific plasma membrane domains of epithelial cells is crucial in the vectorial transfer of sugars. Modification of the transporter genes with the molecular biology techniques and visualization of the expressed gene products by immunohistochemical methods have shed light on the molecular mechanism underlying the specific localization of the transporter molecules.

DOI

Immunolocalization and effect of secretory stimulation

MATSUZAKI TOSHIYUKI, SUZUKI TAKESHI, TAKATA KUNIAKI, Koyama H, Tanaka S

Cell Tissue Res   295,pp513-521  1999

DOI

Aquaporin-5 (AQP5), a water channel protein, in the rat salivary and lacrimal gland : Immunolocalization and effect of secretory stimulation.

MATSUZAKI TOSHIYUKI, SUZUKI TAKESHI, Koyama H, Tanaka S, TAKATA KUNIAKI

Cell Tissue Res   295,pp513-521  1999

DOI

Immunolocalization of water channel protein, aquaporin 3, in the rat urinary system :

MATSUZAKI Toshiyuki, SUZUKI Takeshi, TAKATA Kuniaki

Acta histochemica et cytochemica ( Japan Society of Histochemistry and Cytochemistry )  32 ( 6 ) 546 - 546  1999

CiNii

ラット泌尿器系における水チャネル,アクアポリン3の局在

松崎利行, 鈴木健史, 高田邦昭

日本組織細胞化学会総会・学術集会講演プログラム・予稿集   40th  1999

J-GLOBAL

ラット上皮組織における水チャネル,アクアポリン3

松崎利行, 鈴木健史, 小山晴子, 田中滋康, 高田邦昭

日本細胞生物学会大会講演要旨集   52nd  1999

J-GLOBAL

S VIII-1 細胞膜の糖輸送体SGLT1の局在機構

鈴木 健史, 高田 邦昭

日本組織細胞化学会総会プログラムおよび抄録集 ( 日本組織細胞化学会 )  ( 40 ) 91 - 91  1999

CiNii

Plasma membrane localizization mechanism of the glucose transporter SGLT1 :

Suzuki Takeshi, Takata Kuniaki

Acta histochemica et cytochemica ( Japan Society of Histochemistry and Cytochemistry )  32 ( 6 ) 504 - 504  1999

CiNii

Multiple isoforms of the regulatory subunit for phosphatidylinositol 3-kinase (PI3-kinase) are expressed in neurons in the rat brain

Bo-Chul Shin, Masakazu Suzuki, Kouichi Inukai, Motonobu Anai, Tomoichiro Asano, Kuniaki Takata

Biochemical and Biophysical Research Communications ( Academic Press Inc. )  246 ( 2 ) 313 - 319  1998.05

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Phosphatidylinositol 3-kinase (PI3-kinase) is a heterodimeric enzyme composed of a catalytic subunit of 110 kDa and an adaptor regulatory subunit. We investigated the presence and localization of five isoforms of the regulatory subunits, p55α, p55γ, p85α, p85β, and p50α, in the rat brain. In situ hybridization histochemistry using isoform-specific cRNA probes revealed that all five isoforms were expressed in the neurons of the brain. Interestingly, most neuronal cells including Purkinje cells in the cerebellum and pyramidal cells in the cerebrum expressed all five isoforms. Immuno-histochemical staining also showed the localization of p55α, p55γ, p85α, and p50α in the neuronal cells in the brain. Expression of multiple isoforms in neurons suggests that they may play important roles in signal transduction in the brain.

DOI PubMed CiNii

Direct gene transfer into rat liver cells by in vivo electroporation

Takeshi Suzuki, Bo-Chul Shin, Keiko Fujikura, Toshiyuki Matsuzaki, Kuniaki Takata

FEBS Letters   425 ( 3 ) 436 - 440  1998.04

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In vivo electro-transfection efficiency and manner of transferred gene expression were investigated by fluorescence microscopic image analysis. Green fluorescent protein (GFP) gene was used as the genetic marker, Electroporation was carried out on the liver of live rats by use of disk electrodes mounted in the tips of tweezers, which were directly pressed onto the surface of a liver lobe in situ. Electroporation with eight electric pulses of 50 ms in duration at 50 V gave a good efficiency of transfection as judged by the induced GFP expression. Bright fluorescence of GFP appeared as dots, which were scattered around the area damaged by electroporation. The transfection efficiency increased as the amount of injected DNA was increased. The results indicate that the amount of induced gene expression can be controlled. Estimation of the efficiency of electro-gene transfer using the fluorescence of GFP and digital analysis of microscopic images was useful to determine the optimum conditions for local gene therapy in tissues and organs.

DOI PubMed CiNii

Multiple isoforms of the regulatory subunit for phosphatidylinositol 3-kinase (PI3-kinase) are expressed in neurons in the rat brain.

Shin B-C, SUZUKI TAKESHI, Inukai K, Anai M, Asano T, TAKATA KUNIAKI

Biochem Biophys Res Commun   246 ( 2 ) 313 - 319  1998

DOI PubMed CiNii

ラット胎盤の糖輸送機構．

高田 邦昭, Bo-Chul Shin, 藤倉 恵子, 鈴木 雅一, 鈴木 健史

Mebio   15(No.7),pp 95-97  1998