Misc - SUZUKI Takeshi

GLUT1 glucose transporter in the lactating mammary gland in the rat

K Takata, K Fujikura, M Suzuki, T Suzuki, H Hirano

ACTA HISTOCHEMICA ET CYTOCHEMICA ( JAPAN SOC HISTOCHEMISTRY & CYTOCHEMISTRY )  30 ( 5-6 ) 623 - 628  1997

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Localization of facilitated-diffusion glucose transporter GLUT1 was examined in the rat mammary gland by immunofluorescence microscopy and immunoperoxidase electron microscopy. Secretory epithelial cells of acini were enriched with GLUT1, which was localized to the basolateral plasma membrane. Apical plasma membrane and cytoplasmic organelles including the Golgi apparatus were negative for GLUT1. In addition to acini. abundant GLUT1 was found in the perineurium nerve fiber bundles. which is a site of blood-nerve barriers. GLUT1 in the basolateral membrane of secretory epithelial cells seems to serve as machinery to take up glucose into the cell for milk synthesis and secretion.

DOI CiNii

Nuclear staining for laser confocal microscopy

Toshiyuki Matsuzaki, Takeshi Suzuki, Keiko Fujikura, Kuniaki Takata

Acta Histochemica et Cytochemica ( Japan Society of Histochemistry and Cytochemistry )  30 ( 3 ) 309 - 314  1997

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Twenty nucleic acid-specific fluorescent dyes were screened for use in nuclear staining in laser confocal microscopy. Eleven of them, BO-PRO-1, YO-PRO-1, SYBR Green I, Pico Green, SYTOX Green, TO-PRO-1, POPO3, propidium iodide, YO-PRO-3, TOTO-3, and TO-PRO-3 gave sufficient fluorescence by ingel assay. They were also tested for the nuclear staining in cultured cells. When glyoerol was used as a mounting medium, it enhanced the fluorescence intensity and retarded the bleaching in most of nucleic acid staining dyes. Among green fluorochromes excited at 488nm, YO-PRO-1, SYTOX Green, and SYBR Green I were suitable for nuclear DNA staining. YO-PRO-1 showed the strongest fluorescent intensity, but it weakly costained the cytoplasmic and nucleolar RNAs. SYTOX Green strongly and specifically stained the nuclear DNA. Among red fluorochromes excited at 568nm, propidium iodide stained nuclear DNA with simultaneous staining of RNA. YO-PRO-3 strongly and specifically stained DNA but bleached relatively rapidly. TOPRO-3 excited at 647 nm was specific for DNA, but its fluorescence was bleached rapidly. Selection of nuclear DNA staining dyes should be done by considering the fluorescence intensity, DNA specificity, and bleaching characteristics.

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Glucose transporter Glut3 in the rat placental barrier : A possible machinery for the transplacental transfer of glucose.

Shin B-C, Fujikura K, SUZUKI TAKESHI, Tanaka S, TAKATA KUNIAKI

Endocrinology   138,pp3997-4004  1997

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Structural basis of sugar transport across the placental barrier

TAKATA KUNIAKI, Shin B-C, MATSUZAKI TOSHIYUKI, SUZUKI TAKESHI, Tanaka S, Fujikura K

J Reprod Develop   43 ( Supplement ) 53 - 54  1997

DOI CiNii

Structural basis of sugar transport across the placental barrier

TAKATA KUNIAKI, Shin B-C, MATSUZAKI TOSHIYUKI, SUZUKI TAKESHI, Tanaka S, Fujikura K

J Reprod Develop   43 ( Supplement ) 53 - 54  1997

DOI CiNii

DNA staining for fluorescence and laser confocal microscopy

Takeshi Suzuki, Keiko Fujikura, Tetsuya Higashiyama, Kuniaki Takata

Journal of Histochemistry and Cytochemistry ( Histochemical Society Inc. )  45 ( 1 ) 49 - 53  1997

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We examined five nucleic acid binding fluorescent dyes, propidium iodide, SYBR Green I, YO-PRO-1, TOTO-3, and TO-PRO-3, for nuclear DNA staining, visualized by fluorescence and laser confocal microscopy. The optimal concentration, co-staining of RNA, and bleaching speeds were examined. SYBR Green I and TO-PRO-3 almost preferentially stained the nuclear DNA, and the other dyes co-stained the cytoplasmic RNA. RNAse treatment completely prevented the cytoplasmic RNA staining. In conventional fluorescence microscopy, these dyes can be used in combination with fluorescence-labeled antibodies. Among the dyes tested, TOTO-3 and TO-PRO-3 stained the DNAs with far-red fluorescence under red excitation. Under Kr/Ar- laser illumination, TOTO-3 and TO-PRO-3 were best suited as the nuclear staining dyes in the specimens immunolabeled with fluorescein and rhodamine (or Texas red).

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DNA staining for fluorescence and laser confocal microscopy.

SUZUKI TAKESHI, Keiko Fujikura, Tetsuya Higashiyama, TAKATA KUNIAKI

J Histochem Cytochem   45,pp 49-53  1997

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Glucose transporter Glut3 in the rat placental barrier : A possible machinery for the transplacental transfer of glucose.

Shin B-C, Fujikura K, SUZUKI TAKESHI, Tanaka S, TAKATA KUNIAKI

Endocrinology   138,pp3997-4004  1997

DOI

Fluorescent antibody staining and nucleic acid contrast staining in laser confocal microscope method.

鈴木健史, 松崎利行, 高田邦昭

日本比較内分泌学会ニュース   ( 87 )  1997

J-GLOBAL

共焦点レーザー顕微鏡のための核染色色素の検討

松崎利行, 鈴木健史, 高田邦昭

日本組織細胞化学会総会・学術集会講演プログラム・予稿集   38th  1997

J-GLOBAL

胎盤と物質輸送

高田 邦昭, Bo-Chul Shin, 藤倉 恵子, 田中 滋康, 松崎 利行, 鈴木 健史

産科と婦人科   63,pp1347-1352  1996

Immunolocalization of GLUT1 and connexin 26 in the rat placenta.

Shin B-C, SUZUKI TAKESHI, MATSUZAKI TOSHIYUKI, Tanaka S, Kuraoka A, Shibata Y, TAKATA KUNIAKI

Cell Tissue Res   285,pp83-89  1996

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Immunolocalization of GLUT1 and connexin 26 in the rat placenta.

Shin B-C, SUZUKI TAKESHI, MATSUZAKI TOSHIYUKI, Tanaka S, Kuraoka A, Shibata Y, TAKATA KUNIAKI

Cell Tissue Res   285,pp83-89  1996

DOI

Connexin 43 and the glucose transporter,GLUT1,in the ciliary body of the rat.

Shin B-C, SUZUKI TAKESHI, Tanaka S, Kuraoka A, Shibata Y, TAKATA KUNIAKI

Histochem Cell Biol   106,pp209-214  1996

DOI

Connexin 43 and the glucose transporter,GLUT1,in the ciliary body of the rat.

Shin B-C, SUZUKI TAKESHI, Tanaka S, Kuraoka A, Shibata Y, TAKATA KUNIAKI

Histochem Cell Biol   106,pp209-214  1996

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Variability of mitochondrial subgenomic molecules in the meristematic cells of higher plants.

SUZUKI TAKESHI, Shigeyuki Kawano, Atsushi Sakai, Atsushi Hirai, Tsuneyoshi Kuroiwa

Gen Genet Sys   71 ( 5 ) 329 - 333  1996

DOI PubMed CiNii

Variability of mitochondrial subgenomic molecules in the meristematic cells of higher plants.

SUZUKI TAKESHI, Shigeyuki Kawano, Atsushi Sakai, Atsushi Hirai, Tsuneyoshi Kuroiwa

Gen Genet Sys   71 ( 5 ) 329 - 333  1996

DOI PubMed CiNii

胎盤と物質輸送．

高田 邦昭, Bo-Chul Shin, 藤倉 恵子, 田中 滋康, 松崎 利行, 鈴木 健史

産科と婦人科   63,pp 1347-1352  1996

Organelle DNA synthesis before cell nuclear replication is essential for subsequent cell propagation

Takeshi Suzuki, Atsushi Sakai, Shigeyuki Kawano, Tsuneyoshi Kuroiwa

Cytologia ( Japan Mendel Society )  61 ( 2 ) 235 - 245  1996

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Elevation of intra-cellular organelle DNA levels due to preferential synthesis of organelle DNAs before cell multiplication is generally observed in meristematic cells of higher plants, such as those in apical meristems and cultured cells. We analyzed the physiological significance of this phenomena using cultured tobacco cell BY-2 as a model system for cell multiplication in plants. Cultured tobacco cell BY-2 multiplied approximately 50-fold in a week in normal culture medium. However, when nalidixic acid, which inhibits prokaryotic DNA gyrase, was added to the culture, synthesis of organelle DNA was selectively inhibited and cell multiplication was arrested. This indicates that the synthesis of organelle DNAs is a prerequisite for cell multiplication. To quantitatively clarify the effect of the elevation of organelle DNA levels on the capacity for cell proliferation, cells with various organelle DNA levels were prepared by preculturing cells for various lengths of time in culture medium which contained aphidicolin, a specific inhibitor of eukaryotic DNA polymerase α, and then transferring them to culture medium which contained nalidixic acid. Their growth rates in the absence of further organelle DNA synthesis were monitored. A correlation was observed between intra-cellular organelle DNA levels and the capacity of the cells to proliferate, indicating that intra-cellular organelle DNA levels limited the capacity for cell proliferation.

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Na⁺-dependent glucose transporter SGLT1 is localized in the apical plasma membrane upon completion of tight junction formation in MDCK cells.

SUZUKI TAKESHI, Fujikura K, TAKATA KUNIAKI

Histochem Cell Biol   106,pp529-533  1996

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