Papers - SAITOU Tsuyoshi

子宮体がんスクリーニングにおける遺伝子解析併用の有用性

松浦 基樹, 玉手 雅人, 西尾 空, 西村 庸子, 磯山 響子, 寺田 倫子, 岩崎 雅宏, 齋藤 豪

日本産科婦人科学会雑誌 ( (公社)日本産科婦人科学会 )  73 ( 臨増 ) S - 400  2021.03

病院全体の医療安全を考える サージカルスモークについて

西尾 空, 玉手 雅人, 松浦 基樹, 寺田 倫子, 磯山 響子, 春日 芙貴子, 岡田 匡氷, 齋藤 豪

日本産科婦人科学会雑誌 ( (公社)日本産科婦人科学会 )  73 ( 臨増 ) S - 297  2021.03

術前化学療法が奏功し手術療法が可能となった子宮頸癌IV期病変の摘出子宮における腫瘍浸潤リンパ球(TIL)の特徴を検証した1例

常松 梨紗, 岩崎 雅宏, 郷久 晴朗, 阿部 秀悦, 小川 万梨絵, 黒川 晶子, 西村 庸子, 玉手 雅人, 磯山 響子, 寺田 倫子, 松浦 基樹, 齋藤 豪

日本産科婦人科学会雑誌 ( (公社)日本産科婦人科学会 )  73 ( 臨増 ) S - 313  2021.03

胃原発転移性卵巣腫瘍に対し付属器摘出術を施行した症例の検討

田中 綾一, 辻 靖, 南 妃奈, 清水 亜由美, 池田 詩子, 逸見 博文, 神 崇志, 新藤 智子, 鈴木 善法, 玉手 雅人, 松浦 基樹, 齋藤 豪

北海道産科婦人科学会会誌 ( 北海道産科婦人科学会 )  65 ( 1 ) 43 - 48  2021.03

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転移性卵巣腫瘍は胃癌または大腸癌からの転移が最も多い。胃癌からの転移性卵巣腫瘍は周囲臓器へ浸潤または癒着を認める症例は比較的少なく低侵襲に摘出可能であることが多い。胃原発転移性卵巣腫瘍に対し付属器摘出術を施行した症例について検討した。対象は2017年から2年間の間に胃癌を原発とした転移性卵巣腫瘍に対し付属器摘出術を施行した6例。周術期合併症,術後化学療法開始までの期間,無増悪期間,生存期間,術前術後のQOLの変化等について検討した。胃癌診断時の年齢の中央値は47.0歳,6例中5例に腹腔鏡下手術を選択し,周術期合併症を認めた症例は無かった。術前に下腹部痛を認めた2例は共に術後の症状の消失が確認できた。化学療法再開までの中央値は18.0日,増悪または再発をみとめた3例の無増悪期間はそれぞれ8,2,8ヵ月,付属器摘出術後12,8,12ヵ月に原病死に至った。他の3例は現在いずれも無病生存している。症状緩和目的または卵巣転移以外の病勢が充分に制御されている症例において胃原発転移性卵巣腫瘍に対する付属器摘出術は検討され得ると考えられた。(著者抄録)

Cadaver Surgery Trainingの壁と意義

玉手 雅人, 松浦 基樹, 西尾 空, 太田 未咲, 佐藤 由梨, 野呂 薫, 長尾 沙智子, 阿部 秀悦, 西村 庸子, 磯山 響子, 寺田 倫子, 郷久 晴朗, 岩崎 雅宏, 齋藤 豪

北海道産科婦人科学会会誌 ( 北海道産科婦人科学会 )  65 ( 1 ) 88 - 89  2021.03

Study of laparoscopic monopolar devices and its thermal effects

Motoki Matsuura, Masato Tamate, Sachiko Nagao, Muna Nishio, Tadahi Okada, Yoko Nishimura, Kyoko Isoyama, Noriko Terada, Tsuyoshi Saito

European Journal of Gynaecological Oncology ( IMR Press Limited )  42 ( 1 ) 118 - 121  2021.02

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Objective: Energy devices are frequently used in minimally invasive surgeries (MIS). For MIS involving gynecologic malignancies, energy devices should be used cautiously to prevent thermal injuries to nearby organs. We evaluated monopolar electrosurgery devices and measured increases in temperature in the tissue and device. Methods: Briefly, the surface of a porcine tissue was incised using short and long activation times. Subsequently, the maximum temperature at the tip of the monopolar device, the cooling time required to reach a temperature of 60 ◦C, and the maximum tissue temperature were recorded. Results: Longer activation time was correlated with a higher tip temperature. With all activation times, there was an increase in the tip temperature that exceeded 100 ◦C. The cooling time to reach 60 ◦C was faster with the short activation times than with the long activation times. Even with the same output, the temperature decreased faster in the coagulation mode, suggesting that the cooling times were shorter with lower outputs. The tissue temperature dropped to 60 ◦C or less within 1 s in the cut mode but required approximately 2 s in the coagulation mode at 40 W. The temperature of the dissected tissue increased to 60 ◦C or higher the cooling time was longer with high output and in the coagulation mode. Conclusions: We revealed that the activation of the monopolar device under routine use conditions exceeded a temperature of 100 ◦C. Additionally, the temperatures of the tip and tissue were significantly higher in proportion to the output and time. For MIS involving gynecologic malignancies, careful attention is necessary to avoid thermal injury.

DOI

Aberrant expression of junctional adhesion molecule-A contributes to the malignancy of cervical adenocarcinoma by interaction with poliovirus receptor/CD155.

Taro Murakami, Akira Takasawa, Kumi Takasawa, Taishi Akimoto, Tomoyuki Aoyama, Kazufumi Magara, Yuki Saito, Misaki Ota, Daisuke Kyuno, Soh Yamamoto, Tadashi Hasegawa, Tsuyoshi Saito, Makoto Osanai

Cancer science   112 ( 2 ) 906 - 917  2021.02  [International journal]

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Recent studies have shown that aberrant expression of tight junction proteins (TJP) contributes to malignant potential of various cancers. In the present study, we investigated the expression of junctional adhesion molecule-A (JAM-A), one of the transmembrane TJP, in uterine cervical adenocarcinoma and the significance of its expression for malignancy. Immunohistochemistry on human surgical specimens showed that JAM-A was aberrantly expressed in neoplastic regions including adenocarcinoma in situ (AIS). Knockout of JAM-A significantly suppressed cell proliferation and colony-forming and migration abilities. We also showed that an antibody specific to an extracellular region of JAM-A reduced cell proliferation ability and that loss of JAM-A increased drug sensitivity of cervical adenocarcinoma cells. Based on a comprehensive proteome analysis, we found that poliovirus receptor (PVR/CD155) was regulated by JAM-A and formed a physical interaction with JAM-A. In human surgical specimens, PVR/CD155 expression was significantly correlated with some clinicopathological features and prognosis of cervical adenocarcinoma. Interestingly, most of the PVR/CD155-positive cases expressed a high level of JAM-A, and patients with the expression pattern of PVR/CD155 positive/JAM-A high had significantly shorter periods of relapse-free survival (P = .00964) and overall survival (P = .0204) than those for the other patients. Our observations suggest that aberrant expression of JAM-A promotes malignancy of uterine cervical adenocarcinoma by regulation of PVR/CD155, and JAM-A is therefore a potential therapeutic target for this malignancy.

DOI PubMed

【産婦人科感染症の最新知見】注目されている産婦人科感染症 細菌性腟症、性器クラミジア感染症

島野 敏司, 真里谷 奨, 長井 陽子, 芦川 享大, 荒井 渉, 斎藤 豪

臨床と微生物 ( (株)近代出版 )  48 ( 1 ) 041 - 045  2021.01

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Nugent score 4の亜分類の提唱、帯下Gram染色標本上の検体を16S rRNAシークエンス法の遺伝子解析により、細菌の種を同定した。性器クラミジアは、2010年代後半、日本の生殖年齢女性で増加していた。(著者抄録)

ERα increases endometrial cancer cell resistance to cisplatin via upregulation of BAG3.

Shuetsu Abe, Masahiro Iwasaki, Shutaro Habata, Tasuku Mariya, Masato Tamate, Motoki Matsuura, Seiro Satohisa, Tsuyoshi Saito

Oncology letters   21 ( 1 ) 20 - 20  2021.01  [International journal]

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Endometrial cancer is a leading cause of cancer-associated mortality in women and has a poor prognosis in advanced stages. Our previous study revealed that BCL-2-associated athanogene 3 (BAG3) may contribute to enhancing cell viability through downregulation of microRNA (miR)-29b in endometrial cancer cell lines. In addition, a relationship between estrogen receptor α (ERα) and BAG3 was recently reported in several cancer cell types. The present study investigated the relationship between ERα and BAG3 in endometrial cancer cell lines. The results demonstrated that exogenous ERα overexpression enhanced BAG3 expression in the EMTOKA endometrial cancer cell line, which does not endogenously express ERα, but had no effect on BAG3 expression levels in the Ishikawa cell line, which does endogenously express ERα. In addition, ERα overexpression suppressed miR-29b expression and enhanced the expression of Mcl-1, a mediator situated downstream of BAG3, in EMTOKA cells, but not Ishikawa cells. ERα overexpression also enhanced EMTOKA, but not Ishikawa, endometrial cancer cell viability in the presence of cisplatin. These findings suggested that ERα may contribute to enhancing endometrial cancer cell resistance to anticancer agents through BAG3 overexpression.

DOI PubMed

ERα発現および非発現子宮体癌細胞株に於けるBAG3発現および増殖能との関連

阿部 秀悦, 岩崎 雅宏, 幅田 周太朗, 真里谷 奨, 小川 万梨絵, 郷久 晴朗, 齋藤 豪

日本婦人科腫瘍学会雑誌 ( (公社)日本婦人科腫瘍学会 )  39 ( 1 ) 285 - 285  2021.01

Deep Learning(YOLOv3)を用いたリアルタイム婦人科細胞診断支援システム設計の試み

真里谷 奨, 新開 翔太, 松浦 基樹, 郷久 晴朗, 岩崎 雅宏, 南部 優太, 藤野 雄一, 淺沼 広子, 廣橋 良彦, 鳥越 俊彦, 齋藤 豪

日本婦人科腫瘍学会雑誌 ( (公社)日本婦人科腫瘍学会 )  39 ( 1 ) 278 - 278  2021.01

ERα発現および非発現子宮体癌細胞株に於けるBAG3発現および増殖能との関連

阿部 秀悦, 岩崎 雅宏, 幅田 周太朗, 真里谷 奨, 小川 万梨絵, 郷久 晴朗, 齋藤 豪

日本婦人科腫瘍学会雑誌 ( (公社)日本婦人科腫瘍学会 )  39 ( 1 ) 285 - 285  2021.01

Deep Learning(YOLOv3)を用いたリアルタイム婦人科細胞診断支援システム設計の試み

真里谷 奨, 新開 翔太, 松浦 基樹, 郷久 晴朗, 岩崎 雅宏, 南部 優太, 藤野 雄一, 淺沼 広子, 廣橋 良彦, 鳥越 俊彦, 齋藤 豪

日本婦人科腫瘍学会雑誌 ( (公社)日本婦人科腫瘍学会 )  39 ( 1 ) 278 - 278  2021.01

骨盤解剖学をCadaver Surgery Trainingで理解する混合研究の試み

玉手 雅人, 松浦 基樹, 阿部 秀悦, 西村 庸子, 常松 梨沙, 寺田 倫子, 真里谷 奨, 岩崎 雅宏, 金尾 祐之, 齋藤 豪

日本婦人科腫瘍学会雑誌 ( (公社)日本婦人科腫瘍学会 )  39 ( 1 ) 311 - 311  2021.01

骨盤解剖学をCadaver Surgery Trainingで理解する混合研究の試み

玉手 雅人, 松浦 基樹, 阿部 秀悦, 西村 庸子, 常松 梨沙, 寺田 倫子, 真里谷 奨, 岩崎 雅宏, 金尾 祐之, 齋藤 豪

日本婦人科腫瘍学会雑誌 ( (公社)日本婦人科腫瘍学会 )  39 ( 1 ) 311 - 311  2021.01

タイト結合分子Cluadin-2をターゲットにした子宮内膜癌治療のin vitroにおける検討

嶋田 浩志, 岡田 匡氷, 金野 匠, 幸野 貴之, 松浦 基樹, 郷久 晴朗, 岩崎 雅宏, 小島 隆, 齋藤 豪

日本婦人科腫瘍学会雑誌 ( (公社)日本婦人科腫瘍学会 )  39 ( 1 ) 285 - 285  2021.01

婦人科がんにおける腹腔細胞診の意義 進行卵巣癌における腹水細胞診の意義

松浦 基樹, 西村 庸子, 玉手 雅人, 磯山 響子, 寺田 倫子, 岩崎 雅宏, 磯部 友見子, 畠山 晴良, 高根 希世子, 山口 貴世志, 池上 恒雄, 古川 洋一, 齋藤 豪

日本婦人科腫瘍学会雑誌 ( (公社)日本婦人科腫瘍学会 )  39 ( 1 ) 185 - 185  2021.01

腹腔鏡下子宮筋腫核出術後に発生したparasitic myomaの1例

岩崎 雅宏, 真里谷 奨, 阿部 秀悦, 松浦 基樹, 玉手 雅人, 齋藤 豪

日本産科婦人科内視鏡学会雑誌 ( (一社)日本産科婦人科内視鏡学会 )  36 ( Suppl.I ) [O - 196]  2020.11

YOLOv3を用いた子宮頸部細胞診画像クラスタリング手法の提案

南部 優太, 真里谷 奨, 新開 翔太, 淺沼 広子, 廣橋 良彦, 齋藤 豪, 鳥越 俊彦, 佐藤 生馬, 藤野 雄一

医療情報学連合大会論文集 ( (一社)日本医療情報学会 )  40回   482 - 486  2020.11

Possibility of Targeting Claudin-2 in Therapy for Human Endometrioid Endometrial Carcinoma.

Tadahi Okada, Takumi Konno, Takayuki Kohno, Hiroshi Shimada, Kimihito Saito, Seiro Satohisa, Tsuyoshi Saito, Takashi Kojima

Reproductive sciences (Thousand Oaks, Calif.)   27 ( 11 ) 2092 - 2103  2020.11  [International journal]

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Claudin-2 (CLDN-2) is a leaky-type tight junction protein, and its overexpression increases tumorigenesis of some types of cancer cells. In the present study, to examine the possibility of targeting CLDN-2 in the therapy for endometrioid endometrial adenocarcinoma, we investigated the regulation and role of CLDN-2 in endometriosis and endometrioid endometrial adenocarcinoma. In endometrioid endometrial adenocarcinoma tissues, marked upregulation of CLDN-2 was observed together with malignancy, while in endometriosis tissues, a change in the localization of CLDN-2 was observed. In cells of the endometrial adenocarcinoma cell line Sawano, which highly express CLDN-2, downregulation of CLDN-2 induced by the siRNA upregulated the epithelial barrier and inhibited cell migration. Furthermore, the downregulation of CLDN-2 affected the cell cycle and inhibited cell proliferation. In Sawano cells cultured with high-glucose medium, CLDN-2 expression was downregulated at the mRNA and protein levels. The high-glucose medium upregulated the epithelial barrier, cell proliferation, and migration, and inhibited cell invasion. The histone deacetylase (HDAC) inhibitor tricostatin A (TSA), which has antitumor effects, downregulated CLDN-2 expression, cell proliferation, invasion, and migration, and upregulated the epithelial barrier. The mitochondrial respiration level, an indicator of cancer metabolism, was downregulated by CLDN-2 knockdown and upregulated by the high-glucose condition. Taken together, these results indicated that overexpression of CLDN-2 closely contributed to the malignancy of endometrioid endometrial adenocarcinoma. Downregulation of CLDN-2 via the changes of the glucose concentration and treatment with HDAC inhibitors may be important in the therapy for endometrial cancer.

DOI PubMed