Papers - SAITOU Tsuyoshi

子宮体部漿液性癌18例の検討

寺田 倫子, 松浦 基樹, 西村 庸子, 玉手 雅人, 郷久 晴朗, 岩崎 雅宏, 寺本 瑞絵, 齋藤 豪

日本臨床細胞学会雑誌 ( (公社)日本臨床細胞学会 )  58 ( Suppl.1 ) 276 - 276  2019.05

子宮内膜癌細胞におけるタイト結合分子claudin-2の関与

郷久 晴朗, 嶋田 浩志, 玉手 雅人, 秋元 太志, 西村 庸子, 寺田 倫子, 松浦 基樹, 岩崎 雅宏, 小島 隆, 齋藤 豪

日本臨床細胞学会雑誌 ( (公社)日本臨床細胞学会 )  58 ( Suppl.1 ) 320 - 320  2019.05

早期子宮体癌における腹腔細胞診に関する検討

西村 庸子, 松浦 基樹, 玉手 雅人, 寺田 倫子, 郷久 晴朗, 寺本 瑞絵, 岩崎 雅宏, 齋藤 豪

日本臨床細胞学会雑誌 ( (公社)日本臨床細胞学会 )  58 ( Suppl.1 ) 225 - 225  2019.05

子宮体癌におけるKRAS変異の意義

松浦 基樹, 西村 庸子, 玉手 雅人, 寺田 倫子, 郷久 晴朗, 岩崎 雅宏, 齋藤 豪

日本臨床細胞学会雑誌 ( (公社)日本臨床細胞学会 )  58 ( Suppl.1 ) 319 - 319  2019.05

子宮頸部原発大細胞神経内分泌癌の細胞像

寺本 瑞絵, 西村 庸子, 秋元 太志, 玉手 雅人, 磯山 響子, 小川 万梨絵, 寺田 倫子, 松浦 基樹, 郷久 晴朗, 岩崎 雅宏, 齊藤 豪

日本臨床細胞学会雑誌 ( (公社)日本臨床細胞学会 )  58 ( Suppl.1 ) 270 - 270  2019.05

Androgen potentiates the expression of FSH receptor and supports preantral follicle development in mice.

Yuya Fujibe, Tsuyoshi Baba, Sachiko Nagao, Sayaka Adachi, Keiko Ikeda, Miyuki Morishita, Yoshika Kuno, Masahiro Suzuki, Masahito Mizuuchi, Hiroyuki Honnma, Toshiaki Endo, Tsuyoshi Saito

Journal of ovarian research   12 ( 1 ) 31 - 31  2019.04  [International journal]

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Hyperandrogenism is one of the cardinal symptoms in polycystic ovary syndrome and plays a key role in the pathogenesis of polycystic ovary syndrome. However, the precise effects and mechanisms of excess androgen during follicular development are still unclear. Here we investigated the effects of androgen on mouse follicle development in vitro. Androgen did not affect the growth of follicles smaller than 160-180 μm in the presence of follicle-stimulating hormone (FSH). However, in the presence of low FSH, androgen supported the growth of follicles larger than 160-180 μm, a size at which growing follicles acquire FSH-dependency. Androgen did not change the mRNA expression of various growth-promoting factors but did increase mRNA expression of the FSH receptor. We suggest that androgen has a positive impact on follicle development by augmentation of the actions of FSH. Therefore, FSH-responsive but FSH-independent follicles grow in the presence of a certain level of FSH or androgen, and androgen compensates for FSH deficiency in FSH-dependent follicles.

DOI PubMed

自然消失した子宮動脈仮性動脈瘤の超音波パワードップラー法所見の変化

嶋田 浩志, 野藤 五沙, 鈴木 美紀, 幅田 周太朗, 藤部 佑哉, 水内 将人, 馬場 剛, 石岡 伸一, 齋藤 豪

超音波医学 ( (公社)日本超音波医学会 )  46 ( Suppl. ) S719 - S719  2019.04

出血リスクが高い症例における帝王切開術の工夫

岡田 匡永, 玉手 雅人, 長尾 沙智子, 黒川 晶子, 鈴木 利理, 田渕 雄大, 松浦 基樹, 水内 将人, 岡村 直樹, 齋藤 豪

北海道産科婦人科学会会誌 ( 北海道産科婦人科学会 )  63 ( 1 ) 71 - 71  2019.03

TLHにおいてどうすれば尿管、子宮動脈は簡単に同定できるのか

山下 真祐子, 松浦 基樹, 玉手 雅人, 野呂 薫, 寺田 倫子, 寺本 瑞絵, 齋藤 豪

北海道産科婦人科学会会誌 ( 北海道産科婦人科学会 )  63 ( 1 ) 67 - 67  2019.03

胸郭低形成を契機に診断した6番染色体短腕部分欠失かつ11番染色体部分重複の一例

寺田 倫子[竹田], 寺本 瑞絵, 長尾 沙智子, 玉手 雅人, 磯山 響子, 染谷 真行, 寺田 光次郎, 岡村 直樹, 齋藤 豪

北海道産科婦人科学会会誌 ( 北海道産科婦人科学会 )  63 ( 1 ) 33 - 39  2019.03

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胸郭低形成は、骨系統疾患や羊水過少が原因となって生じることが多い。骨系統疾患では四肢短縮を認めるが、今回胸郭低形成以外に骨所見はなく、羊水過少もない染色体不均衡型転座の症例を経験したので報告する。症例の母体は30歳、7妊2産。妊娠28週で胎児発育不全(fetal growth restriction:FGR)のため近医産婦人科より紹介となった。胎児エコーでは四肢短縮は認めないものの、ベル型の胸郭のため胸郭低形成が疑われた。羊水インデックス(amniotic fluid index:AFI)は5～7cmであった。他、エコースクリーニングでは心房中隔欠損(Atrial septal defect:ASD)疑いとなったが、致死的疾患は認めなかった。TORCH検索では異常は認められなかった。妊娠38週に帝王切開で2,011gの男児が出生し、アプガースコア4/9点、臍帯動脈血ガスpH=7.039であった。出生後、胸郭低形成、ASD、PDA(patent ductus arteriosus)、埋没陰茎、両側難聴などの診断となり、染色体検査に提出したところ46,XY,add(6)(p25)と診断された。両親の染色体を検査に提出したところ、母親が46,XX,t(6;11)(p25;q21)であり、母親由来の染色体不均衡型転座と判明した。児はPDAに対し開胸手術を必要としたが、術後経過順調であり自宅退院となり、3歳の時点で発達の遅れは伴うものの致死的疾患は生じていない。本症例は、6p25欠失と11q部分重複からなる不均衡型転座である。四肢短縮など他の骨所見のない胸郭低形成の症例は、染色体不均衡型転座も考慮する必要がある。(著者抄録)

Clonal analysis revealed functional heterogeneity in cancer stem-like cell phenotypes in uterine endometrioid adenocarcinoma.

Tabuchi Y, Hirohashi Y, Hashimoto S, Mariya T, Asano T, Ikeo K, Kuroda T, Mizuuchi M, Murai A, Uno S, Kawai N, Kubo T, Nakatsugawa M, Kanaseki T, Tsukahara T, Saito T, Torigoe T

Experimental and molecular pathology   106   78 - 88  2019.02  [Refereed]

DOI PubMed

Recurrent Massive Uterine Bleeding from a Cesarean Scar Treated Successfully by Laparoscopic Surgery.

Tasuku Mariya, Mina Umemoto, Naoko Sugita, Masahiro Suzuki, Tsuyoshi Saito

Gynecology and minimally invasive therapy   8 ( 1 ) 36 - 39  2019  [International journal]

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A cesarean scar can cause abnormal uterine bleeding including prolonged menstruation or postmenstrual spotting. Our patient showed massive uterine bleeding from a cesarean scar and needed blood transfusion for hemorrhagic shock. A cesarean section had only been performed once for delivery stop 9 years ago. Recurrent hemorrhage could not be controlled by conservative treatment, and we performed laparoscopic scar resection and repair. The abnormal uterine bleeding was successfully stopped, and the menstrual cycle was normalized after surgical treatment. We should be aware that even an uneventful cesarean section may have a risk of massive hemorrhage postoperatively as in the present case.

DOI PubMed

The roles of metformin and pravastatin on Placental endoplasmic reticulum stress and Placental growth factor in human villous-like trophoblastic BeWo cells

Masahiro Suzuki, Masahito Mizuuchi, Tsuyoshi Baba, Yuya Fujibe, Yoshihiko Ino, Shinichi Ishioka, Tsuyoshi Saito

Sapporo Medical journal ( Sapporo Medical University )  87 ( 1-6 ) 75 - 84  2018.12

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Preeclampsia (PE) is related to an imbalance of angiogenic factors. Placental growth factor (P1GF) is reduced in early onset PE. Recent evidence indicates that metformin and statins may suppress endoplasmic reticulum (ER) stress. We previously reported that the unfolded protein response activated by ER stress down-regulated P1GF protein expression and predicted these drugs could prevent placental ER stress and up-regulate P1GF protein expression in trophoblast-like cells. In this study, we aimed to establish the effects of these drugs on P1GF and activating transcription factor 4 (ATF4) protein expression. We confirmed that P1GF messenger RNA (mRNA) levels were decreased under ER stress induced by thapsigargin and that ATF4 mRNA was increased under the same conditions and then administered metformin to it. Transcript analysis showed increased P1GF mRNA compared with thapsigargin only treatment and that this was dependent on metformin levels. Under ER stress, western blot showed high levels of ATF4 and phosphorylated-eukaryotic initiation factor 2 subunit a (p-eIF2a) but low levels of P1GF protein. By contrast, compared with thapsigargin alone, ATF4 and p-eIF2a levels were low and P1GF levels were high when metformin and thapsigargin were given, but these were again dependent on metformin concentrations. Western blot also confirmed that pravastatin attenuated ER stress and increased P1GF protein expression. In conclusion, metformin and pravastatin suppressed ER stress and restored P1GF levels in trophoblastlike cells. However, although these results indicate that these drugs have a potential for preventing or treating PE, the lack of clarity on the mechanism requires further study.

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Efficacy of liquid-based genetic diagnosis of endometrial cancer.

Matsuura M, Yamaguchi K, Tamate M, Satohisa S, Teramoto M, Iwasaki M, Sugita S, Hasegawa T, Koubo R, Takane K, Ikenoue T, Furukawa Y, Saito T

Cancer science   109 ( 12 ) 4025 - 4032  2018.12  [Refereed]

DOI PubMed

TLHにおけるデバイスの選択について

西村 庸子, 松浦 基樹, 石堂 茉泉, 長尾 沙智子, 玉手 雅人, 齋藤 豪

日本内視鏡外科学会雑誌 ( (一社)日本内視鏡外科学会 )  23 ( 7 ) OS139 - 3  2018.12

子宮体癌に対するロボット支援下子宮全摘術を円滑に行うために

松浦 基樹, 石堂 茉泉, 長尾 沙智子, 西村 庸子, 玉手 雅人, 齋藤 豪

日本内視鏡外科学会雑誌 ( (一社)日本内視鏡外科学会 )  23 ( 7 ) OS142 - 5  2018.12

Cytotoxicity of Clostridium perfringens enterotoxin depends on the conditions of claudin-4 in ovarian carcinoma cells.

Satoshi Tanaka, Tomoyuki Aoyama, Marie Ogawa, Akira Takasawa, Masaki Murata, Makoto Osanai, Tsuyoshi Saito, Norimasa Sawada

Experimental cell research   371 ( 1 ) 278 - 286  2018.10  [International journal]

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Currently, Clostridium perfringens enterotoxin (CPE) is being investigated as an anti-cancer drug for tumors expressing the tight junction (TJ) transmembrane proteins claudin-3 and/or claudin-4. However, the optimal conditions for CPE cytotoxicity are still unclear. Our objectives were to determine the optimal conditions for CPE as an anti-cancer drug for treating ovarian cancer in vitro and in vivo. In our experiments, cells at low culture density showed higher sensitivity to CPE, suggesting that claudins at TJs were poorly accessible to CPE compared with those at the edge of cell colonies. Ovarian cancer cells cultured under calcium-depleted pretreatment conditions to disrupt TJs and to knock-down TJ proteins and E-cadherin production altered CPE cytotoxicity, which was mainly dependent on claudin-4 expression. These results suggest that the condition of claudin-4 at the cell surface is important for CPE cytotoxicity. Our in vivo experiments showed that a high dose of CPE is required for the effective treatment of peritoneal dissemination of ovarian cancer cells. Here, we suggest that the accessibility of CPE to claudins is important for its cytotoxicity and depends on the conditions of claudin-4 in vitro. In addition, E-cadherin expression in ovarian cancer cells affects the efficiency of CPE in vivo.

DOI PubMed

Treatment and prognosis of recurrent uterine leiomyosarcomas in last 5 years

Saito Tsuyoshi, Tanaka Rhoichi, Satohisa Seiro, Abe Shuetsu, Iwasaki Masahiro, Teramoto Mizue, Matsuura Motoki, Tamate Masato

INTERNATIONAL JOURNAL OF GYNECOLOGICAL CANCER   28   50  2018.10  [Refereed]

Estrogen/GPR30 Signaling Contributes to the Malignant Potentials of ER-Negative Cervical Adenocarcinoma via Regulation of Claudin-1 Expression.

Taishi Akimoto, Akira Takasawa, Kumi Takasawa, Tomoyuki Aoyama, Masaki Murata, Makoto Osanai, Tsuyoshi Saito, Norimasa Sawada

Neoplasia (New York, N.Y.)   20 ( 10 ) 1083 - 1093  2018.10  [International journal]

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Cervical adenocarcinomas are believed to lose estrogen response on the basis of no expression of a nuclear estrogen receptor such as ERα in clinical pathology. Here, we demonstrated that cervical adenocarcinoma cells respond to a physiological concentration of estrogen to upregulate claudin-1, a cell surface molecule highly expressed in cervical adenocarcinomas. Knockout of claudin-1 induced apoptosis and significantly inhibited proliferation, migration, and invasion of cervical adenocarcinoma cells and tumorigenicity in vivo. Importantly, all of the cervical adenocarcinoma cell lines examined expressed a membrane-bound type estrogen receptor, G protein-coupled receptor 30 (GPR30/GPER1), but not ERα. Estrogen-dependent induction of claudin-1 expression was mediated by GPR30 via ERK and/or Akt signaling. In surgical specimens, there was a positive correlation between claudin-1 expression and GPR30 expression. Double high expression of claudin-1 and GPR30 predicts poor prognosis in patients with cervical adenocarcinomas. Mechanism-based targeting of estrogen/GPR30 signaling and claudin-1 may be effective for cervical adenocarcinoma therapy.

DOI PubMed

Multiple part umbilical cord entanglement and neonatal outcomes

Tasuku Mariya, Yuya Fujibe, Shota Shinkai, Naoko Sugita, Masahiro Suzuki, Toshiaki Endo, Tsuyoshi Saito

Taiwanese Journal of Obstetrics and Gynecology ( Elsevier BV )  57 ( 5 ) 672 - 676  2018.10

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