Details of a Researcher - SAITOU Tsuyoshi

Papers - SAITOU Tsuyoshi

Division display 341 - 360 of about 473 ／ All the affair displays >>

明石祐史, 杉尾明香, 西澤庸子, 玉手雅人, 金美善, 足立英文, 森下美幸, 齋藤豪

北海道産科婦人科学会会誌 ( 北海道産科婦人科学会 ) 61 ( 1 ) 56 - 56 2017.03

竹田倫子[寺田], 玉手雅人, 杉田真太朗, 鈴木孝浩, 齋藤豪

日本臨床細胞学会雑誌 ( (公社)日本臨床細胞学会 ) 56 ( 2 ) 92 - 95 2017.03

背景:子宮平滑筋肉腫は子宮体部に発生することが多く、子宮頸部原発の平滑筋肉腫はまれである。われわれは、子宮頸部に発生した平滑筋肉腫の症例を経験したので報告する。症例:症例は45歳。不正出血が続くため当科を受診した。子宮頸部に1.5cm大の筋腫様腫瘤を認めた。腫瘍擦過細胞診ではNILM(negative for intraepithelial lesion or malignancy)と診断し、子宮鏡下に腫瘍摘出術を施行したところ平滑筋肉腫との診断にて、初回手術から23日後に準広汎子宮全摘出術+両付属器摘出術を施行した。術後約2年経過したが、今のところ再発の徴候はない。結論:平滑筋肉腫は細胞診で診断することが困難な場合があるため、細胞診で紡錘形細胞を認めた場合は、悪性腫瘍である可能性も考慮しながら診断を進めていく必要がある。(著者抄録)

腹腔鏡下子宮筋腫核出術において、筋腫のバリエーションから考えた術前治療と術後病理

玉手雅人, 長尾沙智子, 染谷真行, 竹田倫子, 杉尾明香, 森下美幸, 明石祐史, 岡村直樹, 齋藤豪

北海道産科婦人科学会会誌 ( 北海道産科婦人科学会 ) 61 ( 1 ) 55 - 55 2017.03

Loss of tricellular tight junction protein LSR promotes cell invasion and migration via upregulation of TEAD1/AREG in human endometrial cancer

Hiroshi Shimada, Shyuetsu Abe, Takayuki Kohno, Seiro Satohisa, Takumi Konno, Syunta Takahashi, Tsubasa Hatakeyama, Chihiro Arimoto, Takuya Kakuki, Yakuto Kaneko, Ken-ichi Takano, Tsuyoshi Saito, Takashi Kojima

SCIENTIFIC REPORTS ( NATURE PUBLISHING GROUP ) 7 2017.01 [Refereed]

Lipolysis-stimulated lipoprotein receptor (LSR) is a unique molecule of tricellular contacts of normal and cancer cells. We investigated how the loss of LSR induced cell migration, invasion and proliferation in endometrial cancer cell line Sawano. mRNAs of amphiregulin (AREG) and TEA domain family member 1 (TEAD1) were markedly upregulated by siRNA-LSR. In endometrial cancer tissues, downregulation of LSR and upregulation of AREG were observed together with malignancy, and Yes-associated protein (YAP) was present in the nuclei. siRNA-AREG prevented the cell migration and invasion induced by siRNA-LSR, whereas treatment with AREG induced cell migration and invasion. LSR was colocalized with TRIC, angiomotin (AMOT), Merlin and phosphorylated YAP (pYAP). siRNA-LSR increased expression of pYAP and decreased that of AMOT and Merlin. siRNA-YAP prevented expression of the mRNAs of AREG and TEAD1, and the cell migration and invasion induced by siRNA-LSR. Treatment with dobutamine and 2-deoxy-D-glucose and glucose starvation induced the pYAP expression and prevented the cell migration and invasion induced by siRNA-LSR. siRNA-AMOT decreased the Merlin expression and prevented the cell migration and invasion induced by siRNA-LSR. The loss of LSR promoted cell invasion and migration via upregulation of TEAD1/AREG dependent on YAP/pYAP and AMOT/Merlin in human endometrial cancer cells.

DOI PubMed

Prognostic significance of the co-expression of EGFR and HER2 in adenocarcinoma of the uterine cervix.

Ueda A, Takasawa A, Akimoto T, Takasawa K, Aoyama T, Ino Y, Nojima M, Ono Y, Murata M, Osanai M, Hasegawa T, Saito T, Sawada N

PLOS ONE 12 ( 8 ) e0184123 2017 [Refereed]

DOI PubMed

Downregulation of LSR promotes cell invasion via claudin-1-mediated MMPs in human endometrial cancer.

Shimada H, Satohisa S, Kohno T, Konno T, Takano K, Takahashi S, Hatakeyama T, Arimoto C, Saito T, Kojima T

Oncology Letters 14(6):6776-6782. 2017 [Refereed]

PubMed

Virological and cytological clearance in laser vaporization and conization for cervical intra-epithelial neoplasia grade 3.

Tasuku Mariya, Akira Nishikawa, Kanae Sogawa, Riri Suzuki, Masae Saito, Akari Kawamata, Ayumi Shimizu, Takehito Nihei, Tomoko Sonoda, Tsuyoshi Saito

The journal of obstetrics and gynaecology research 42 ( 12 ) 1808 - 1813 2016.12 [International journal]

AIM: Cervical intra-epithelial neoplasia (CIN) is the precancerous stage of cervical cancer. Standard treatment for high-grade CIN is conization of the cervix. The risk of preterm birth following conization has been discussed recently. In contrast, laser vaporization is believed not to affect perinatal outcome, but the long-term effectiveness of each surgical procedure is still unclear. The aim of this prospective unmatched-cohort study was therefore to compare virological and cytological clearance and recurrence risk between conization and vaporization for CIN3. METHODS: Subject consisted of CIN3 patients treated at the present hospital between 2007 to 2011 and followed up until December 2014. One hundred and one patients were treated with laser conization, and 137 with vaporization. The surgical procedure was selected on the basis of colposcopy, pathological grade and patient's hope for pregnancy. RESULTS: There were no significant differences in cure rate, human papilloma virus (HPV) clearance rate or recurrence rates between the conization and vaporization groups. Risk ratio of recurrence for each surgical procedure adjusted for age and HPV persistence status were analyzed on Cox proportional hazards modeling. Recurrence risk ratio for patients treated by vaporization was 6.21 (95%CI: 0.65-59.19; P = 0.111) compared with conization and there were no significant differences. No adverse pregnancy outcome was observed in the vaporization group compared with conization. CONCLUSIONS: Laser vaporization is useful for young patients with CIN3 who hope for pregnancy in the future.

DOI PubMed

Influence of Ku86 and XRCC4 expression in uterine cervical cancer on the response to preoperative radiotherapy

Yu Takada, Masanori Someya, Yoshihisa Matsumoto, Masaaki Satoh, Kensei Nakata, Masakazu Hori, Masato Saito, Naoki Hirokawa, Kunihiko Tateoka, Mizue Teramoto, Tsuyoshi Saito, Tadashi Hasegawa, Koh-ichi Sakata

MEDICAL MOLECULAR MORPHOLOGY ( SPRINGER JAPAN KK ) 49 ( 4 ) 210 - 216 2016.12 [Refereed]

DNA double-strand breaks (DSB) are severe damages induced by ionizing radiation. Non-homologous end joining (NHEJ) is a major mechanism for repairing DSB. Immunohistochemical analysis of proteins involved in NHEJ, such as Ku86 and XRCC4 (X-ray repair cross-complementing protein 4) may be useful for predicting tumor radiosensitivity. We examined the relationship between expression of DSB-related proteins in biopsy specimens of uterine cervical cancer and the pathological effect of 40 Gy of preoperative radiotherapy. 119 patients with uterine cervical cancer were treated between 2000 and 2011. Pathological effects of preoperative radiotherapy were classified by examining hysterectomy specimens. Patients with complete response (pCR) had a significantly better overall 5-year survival rate than those without pCR (96.3 vs. 76.9 %, P = 0.02). The pCR rate was significantly higher in patients with low Ku86 and XRCC4 expression than in other patients (47.4 vs. 21.3 %, P = 0.04). Logistic regression analysis also demonstrated that low Ku86 and XRCC4 expression was a significant predictor of pCR (P = 0.03). Patients with high Ku86 and XRCC4 expression had a significantly lower 5-year metastasis-free rate than others (79.3 vs. 93.5 %, P = 0.02). Proteins involved with NHEJ might have an influence on results of radiotherapy for uterine cervical cancer.

DOI

腹水セルブロックにおける検討が治療方針決定に有用であった腹膜原発粘液性腺癌の1例

玉手雅人, 長尾沙智子, 竹田倫子, 松浦基樹, 寺本瑞絵, 郷久晴朗, 田中綾一, 岩崎雅宏, 齋藤豪

日本臨床細胞学会雑誌 ( (公社)日本臨床細胞学会 ) 55 ( Suppl.2 ) 494 - 494 2016.10

Analysis of the expression and localization of tight junction transmembrane proteins, claudin-1,-4,-7, occludin and JAM-A, in human cervical adenocarcinoma

Taishi Akimoto, Akira Takasawa, Masaki Murata, Yui Kojima, Kumi Takasawa, Masanori Nojima, Tomoyuki Aoyama, Yutaro Hiratsuka, Yusuke Ono, Satoshi Tanaka, Makoto Osanai, Tadashi Hasegawa, Tsuyoshi Saito, Norimasa Sawada

HISTOLOGY AND HISTOPATHOLOGY ( F HERNANDEZ ) 31 ( 8 ) 921 - 931 2016.08 [Refereed]

Objective. Tight junction proteins have recently been reported to be useful for distinguishing between neoplastic and non-neoplastic tissues. In this study, we evaluated the expression and localization of tight junction transmembrane proteins in human cervical adenocarcinoma and adenocarcinoma in situ (AIS), and we determined whether their expression patterns could distinguish cervical adenocarcinoma from nonneoplastic cervical glands. Methods. Fifty-five patients with cervical adenocarcinoma or AIS were included in this study. Surgical specimens were immunohistochemically stained for claudin (CLDN) -1, -4, -7, occludin, and JAM-A. Results. Significantly higher expression levels of CLDNs and JAM-A were found in cervical AIS and adenocarcinoma than in non-neoplastic glands. In cervical AIS and adenocarcinoma, localization of CLDN-1 and JAM-A was extended throughout the whole cell membranes, whereas they were predominantly expressed at the most apical cell-cell junction in non-neoplastic glands. ROC curve analysis revealed that immunoreactivities of CLDN-1 or JAM-A successfully distinguished neoplasms from nonneoplastic cervical glands with high specificity (CLDN-1, 79.1%; JAM-A, 79.1%) and high sensitivity (CLDN-1, 84.1%; JAM-A, 95.5%). Conclusions. As expected, there were immunohistochemical differences between cervical adenocarcinoma and non-neoplastic cervical glands by using antibodies against tight junction transmembrane proteins. These results suggest that CLDN-1 and JAM-A are potential biomarkers for cervical adenocarcinoma.

DOI

腹腔鏡下に診断し得た女児の左卵管留血腫

鹿内智史, 嶋田浩志, 真里谷奨, 郷久晴朗, 高橋徹, 齋藤豪

日本産科婦人科内視鏡学会雑誌 ( (一社)日本産科婦人科内視鏡学会 ) 32 ( Suppl.I ) 246 - 246 2016.08

子宮全摘出術における腹腔鏡下手術と腟式手術の手術侵襲に関する検討

田中綾一, 明石祐史, 玉手雅人, 杉尾明香, 金美善, 寺田倫子, 森下美幸, 郷久晴朗, 寺本瑞絵, 岩崎雅宏, 齋藤豪

日本産科婦人科内視鏡学会雑誌 ( (一社)日本産科婦人科内視鏡学会 ) 32 ( Suppl.I ) 322 - 322 2016.08

腹腔鏡下手術においてセプラフィルム腹腔内搬入および貼付目的に用いたトロックスガーゼの使用経験

田中綾一, 森下美幸, 寺本瑞絵, 寺田倫子, 玉手雅人, 齋藤豪

日本産科婦人科内視鏡学会雑誌 ( (一社)日本産科婦人科内視鏡学会 ) 32 ( Suppl.I ) 324 - 324 2016.08

Possibility of less radical treatment for patients with early invasive uterine cervical cancer

Miseon Kim, Shinichi Ishioka, Toshiaki Endo, Tsuyoshi Baba, Masahito Mizuuchi, Sakura Takada, Tsuyoshi Saito

JOURNAL OF OBSTETRICS AND GYNAECOLOGY RESEARCH ( WILEY-BLACKWELL ) 42 ( 7 ) 876 - 882 2016.07 [Refereed]

AimRadical trachelectomy (RT) with lymphadenectomy has become a standard treatment modality for patients with early invasive uterine cervical cancer who hope to preserve fertility. However, pregnancy after RT has high risks of preterm birth. The possibility of more conservative RT and the application of RT for patients with higher clinical stages were studied. MethodsThe medical charts and specimens of 42 patients who underwent RT and 64 patients who underwent radical hysterectomy were retrospectively studied. Tumor size, distance between the margin of the cancer and the internal orifice of the uterus (os), parametrial invasion, lymph node metastasis and prognoses were investigated. ResultsThe average distances between the inner margin of the cancer and the internal os were 37 mm, 29 mm, 18.7 mm and 14 mm for patients with stage 1 A2, 1B1 ( 2 cm), 1B1 (> 2 cm) and 1B2, respectively. When amputation was performed 10 mm below the internal os, all 10 patients with 1 A2, 57 with 1B1 ( 2 cm), 19 with 1B1 (> 2 cm), and one with 1B2 had a cancer-free margin > 10 mm. Patients with stage 1 A2 had a cancer-free margin > 10 mm even if we amputated the cervix 20 mm below the internal os. Parametrial invasion was detected in two patients with stage 1B1. ConclusionsA simple trachelectomy 20 mm below the internal os with pelvic lymphadenectomy might be possible for stage 1 A2 patients. The present method is best for stage 1B1 patients ( 2 cm). RT for stage 1B1 (>2 cm) or higher stages should be contraindicated.

DOI

BAG3-mediated Mcl-1 stabilization contributes to drug resistance via interaction with USP9X in ovarian cancer

Shutaro Habata, Masahiro Iwasaki, Asuka Sugio, Miwa Suzuki, Masato Tamate, Seiro Satohisa, Ryoichi Tanaka, Tsuyoshi Saito

INTERNATIONAL JOURNAL OF ONCOLOGY ( SPANDIDOS PUBL LTD ) 49 ( 1 ) 402 - 410 2016.07 [Refereed]

Paclitaxel in combination with carboplatin improves survival among patients with susceptible ovarian cancers, but no strategy has been established against resistant ovarian cancers. BAG3 (Bcl-2-associated athanogene 3) is one of six BAG family proteins, which are involved in such cellular processes as proliferation, migration and apoptosis. In addition, expression of BAG3 with Mcl-1, a Bcl-2 family protein, reportedly associates with resistance to chemotherapy. Our aim in this study was to evaluate the functional role of BAG3 and Mcl-1 in ovarian cancer chemoresistance and explore possible new targets for treatment. We found that combined expression of BAG3 and Mcl-1 was significantly associated with a poor prognosis in ovarian cancer patients. In vitro, BAG3 knockdown in ES2 clear ovarian cancer cells significantly increased the efficacy of paclitaxel in combination with the Mcl-1 antagonist MIM1, with or without the Bcl-2 family antagonist ABT737. Moreover, BAG3 was found to positively regulate Mcl-1 levels by binding to and inhibiting USP9X. Our data show that BAG3 and Mcl-1 are key mediators of resistance to chemotherapy in ovarian cancer. In BAG3 knockdown ES2 clear ovarian cancer cells, combination with ABT737 and MIM1. enhanced the efficacy of paclitaxel. These results suggest that inhibiting BAG3 in addition to antiapoptotic Bcl-2 family proteins may be a useful therapeutic strategy for the treatment of chemoresistant ovarian cancers.

DOI

3細胞間タイト結合蛋白LSRはHippo pathwayを介して子宮内膜癌の悪性化に関与している

嶋田浩志, 郷久晴朗, 金野匠, 高橋駿太, 畠山翔翼, 幸野貴之, 田中綾一, 寺本瑞絵, 岩崎雅宏, 小島隆, 齋藤豪

日本婦人科腫瘍学会雑誌 ( (公社)日本婦人科腫瘍学会 ) 34 ( 3 ) 416 - 416 2016.06

Matrix metalloproteinase-10 regulates stemness of ovarian cancer stem-like cells by activation of canonical Wnt signaling and can be a target of chemotherapy-resistant ovarian cancer

Tasuku Mariya, Yoshihiko Hirohashi, Toshihiko Torigoe, Yuta Tabuchi, Takuya Asano, Hiroshi Saijo, Takafumi Kuroda, Kazuyo Yasuda, Masahito Mizuuchi, Tsuyoshi Saito, Noriyuki Sato

ONCOTARGET ( IMPACT JOURNALS LLC ) 7 ( 18 ) 26806 - 26822 2016.05 [Refereed]

Epithelial ovarian cancer (EOC) is one of the most lethal cancers in females. Cancer stem-like cells (CSCs)/cancer-initiating cells (CICs) have been reported to be origin of primary and recurrent cancers and to be resistant to several treatments. In this study, we identified matrix metalloproteinase-10 (MMP10) is expressed in CSCs/CICs of EOC. An immunohistochemical study revealed that a high expression level of MMP10 is a marker for poor prognosis and platinum resistance in multivariate analysis. MMP10 gene overexpression experiments and MMP10 gene knockdown experiments using siRNAs revealed that MMP10 has a role in the maintenance of CSCs/CICs in EOC and resistance to platinum reagent. Furthermore, MMP10 activate canonical Wnt signaling by inhibiting noncanonical Wnt signaling ligand Wnt5a. Therefore, MMP10 is a novel marker for CSCs/CICs in EOC and that targeting MMP10 is a novel promising approach for chemotherapy-resistant CSCs/CICs in EOC.

DOI

MAPK13 is preferentially expressed in gynecological cancer stem cells and has a role in the tumor-initiation.

Kazuyo Yasuda, Yoshihiko Hirohashi, Takafumi Kuroda, Akari Takaya, Terufumi Kubo, Takayuki Kanaseki, Tomohide Tsukahara, Tadashi Hasegawa, Tsuyoshi Saito, Noriyuki Sato, Toshihiko Torigoe

Biochemical and biophysical research communications 472 ( 4 ) 643 - 7 2016.04 [International journal]

Cancer stem-like cells (CSCs)/cancer-initiating cells (CICs) are defined as small subpopulation of cancer cells that are endowed with higher tumor-initiating ability. CSCs/CICs are resistant to standard cancer therapies including chemotherapy and radiotherapy, and they are thus thought to be responsible for cancer recurrence and metastasis. Therefore, elucidation of molecular mechanisms of CSCs/CICs is essential to cure cancer. In this study, we analyzed the gene expression profiles of gynecological CSCs/CICs isolated as aldehyde dehydrogenase high (ALDH(high)) cells, and found that MAPK13, PTTG1IP, CAPN1 and UBQLN2 were preferentially expressed in CSCs/CICs. MAPK13 is expressed in uterine, ovary, stomach, colon, liver and kidney cancer tissues at higher levels compared with adjacent normal tissues. MAPK13 gene knockdown using siRNA reduced the ALDH(high) population and abrogated the tumor-initiating ability. These results indicate that MAPK13 is expressed in gynecological CSCs/CICs and has roles in the maintenance of CSCs/CICs and tumor-initiating ability, and MAPK13 might be a novel molecular target for treatment-resistant CSCs/CICs.

DOI PubMed

子宮体癌初代培養細胞株における癌幹細胞の分離同定とその解析

田渕雄大, 廣橋良彦, 真里谷奨, 浅野拓也, 黒田敬史, 齋藤豪, 鳥越俊彦

日本病理学会会誌 ( (一社)日本病理学会 ) 105 ( 1 ) 397 - 398 2016.04

Brother of the regulator of the imprinted site (BORIS) variant subfamily 6 is involved in cervical cancer stemness and can be a target of immunotherapy

Takuya Asano, Yoshihiko Hirohashi, Toshihiko Torigoe, Tasuku Mariya, Ryota Horibe, Takafumi Kuroda, Yuta Tabuchi, Hiroshi Saijo, Kazuyo Yasuda, Masahito Mizuuchi, Akari Takahashi, Hiroko Asanuma, Tadashi Hasegawa, Tsuyoshi Saito, Noriyuki Sato

ONCOTARGET ( IMPACT JOURNALS LLC ) 7 ( 10 ) 11223 - 11237 2016.03 [Refereed]

Cervical cancer is a major cause of cancer death in females worldwide. Cervical cancer stem-like cells (CSCs)/cancer-initiating cells (CICs) are resistant to conventional radiotherapy and chemotherapy, and CSCs/CICs are thought to be responsible for recurrence. Eradication of CSCs/CICs is thus essential to cure cervical cancer. In this study, we isolated cervical CSCs/CICs by sphere culture, and we identified a cancer testis (CT) antigen, CTCFL/BORIS, that is expressed in cervical CSCs/CICs. BORIS has 23 mRNA isoform variants classified by 6 subfamilies (sfs), and they encode 17 different BORIS peptides. BORIS sf1 and sf4 are expressed in both CSCs/CICs and non-CSCs/CICs, whereas BORIS sf6 is expressed only in CSCs/CICs. Overexpression of BORIS sf6 in cervical cancer cells increased sphere formation and tumor-initiating ability compared with those in control cells, whereas overexpression of BORIS sf1 and BORIS sf4 resulted in only slight increases. Thus, BORIS sf6 is a cervical CSC/CIC-specific subfamily and has a role in the maintenance of cervical CSCs/CICs. BORIS sf6 contains a specific c-terminal domain (C34), and we identified a human leukocyte antigen (HLA)-A2-restricted antigenic peptide, BORIS C34_24(9) encoded by BORIS sf6. A BORIS C34_24(9)-specific cytotoxic T cell (CTL) clone showed cytotoxicity for BORIS sf6-overexpressing cervical cancer cells. Furthermore, the CTL clone significantly suppressed sphere formation of CaSki cells. Taken together, the results indicate that the CT antigen BORIS sf6 is specifically expressed in cervical CSCs/CICs, that BORIS sf6 has a role in the maintenance of CSCs/CICs, and that BORIS C34_24(9) peptide is a promising candidate for cervical CSC/CIC-targeting immunotherapy.

　 PREV - NEXT 　

<SAITOU Tsuyoshi>

Papers - SAITOU Tsuyoshi