Papers - SAITOU Tsuyoshi

当院における再発子宮体癌に対するPembrolizumab+Lenvatinibの使用経験

長尾 沙智子, 松浦 基樹, 黒川 晶子, 玉手 雅人, 秋元 太志, 幅田 周太朗, 岩崎 雅宏, 齋藤 豪

日本婦人科腫瘍学会学術講演会プログラム・抄録集 ( (公社)日本婦人科腫瘍学会 )  66回   405 - 405  2024.07

The roles of tight junction protein cingulin in human endometrioid endometrial cancer.

Arisa Kura, Kimihito Saito, Takumi Konno, Takayuki Kohno, Hiroshi Shimada, Tadahi Okada, Soshi Nishida, Daichi Ishii, Motoki Matsuura, Tsuyoshi Saito, Takashi Kojima

Tissue barriers     2361976 - 2361976  2024.06  [International journal]

　View Summary

The bicellular tight junction molecule cingulin (CGN) binds to microtubules in centrosomes. Furthermore, CGN contributes to the tricellular tight junction (tTJ) proteins lipolysis-stimulated lipoprotein receptor (LSR) and tricellulin (TRIC). CGN as well as LSR decreased during the malignancy of endometrioid endometrial cancer (EEC). Although tTJ protein LSR is involved in the malignancy of some cancers, including EEC, the role of CGN is unknown. In this study, we investigated the roles of CGN with tTJ proteins in human EEC cells by using the CGN-overexpressing EEC cell line Sawano. In 2D cultures, CGN was colocalized with LSR and TRIC at tTJ or at γ-tubulin-positive centrosomes. In immunoprecipitation with CGN antibodies, CGN directly bound to LSR, TRIC, and β-tubulin. Knockdown of CGN by the siRNA decreased the epithelial barrier and enhanced cell proliferation, migration and invasion, as well as knockdown of LSR. In the Sawano cells cocultured with normal human endometrial stromal cells, knockdown of CGN decreased expression of LSR and TRIC via MAPK and AMPK pathways. In 2.5D cultures, knockdown of CGN induced the formation of abnormal cysts and increased the permeability of FD-4 to the lumen. In 2D and 2.5D cultures, treatment with β-estradiol with or without EGF or TGF-β decreased CGN expression and the epithelial permeability barrier and enhanced cell migration, and pretreatment with EW7197+AG1478, U0126 or an anti-IL-6 antibody prevented this. In conclusion, CGN, with tTJ proteins might suppress the malignancy of human EEC and its complex proteins are sensitive to estrogen and growth factors derived from stromal cells.

DOI PubMed

妊娠後期に急性腹症と肝酵素上昇を認め,総胆管結石として待機的管理を行った1例

宮下 夕稀子, 染谷 真行, 真里谷 奨, 馬場 剛, 石岡 伸一, 岡村 直樹, 齋藤 豪

日本周産期・新生児医学会雑誌 ( (一社)日本周産期・新生児医学会 )  60 ( Suppl.1 ) P395 - P395  2024.06

低置胎盤の分娩管理の変化と,それに伴う周産期転帰の変化について

染谷 真行, 古来 愛香, 真里谷 奨, 馬場 剛, 石岡 伸一, 齋藤 豪

日本周産期・新生児医学会雑誌 ( (一社)日本周産期・新生児医学会 )  60 ( Suppl.1 ) P382 - P382  2024.06

妊娠中期に多量出血を伴わずに原因不明の産科DICを発症した2例

齋藤 晋平, 染谷 真行, 真里谷 奨, 馬場 剛, 石岡 伸一, 齋藤 豪

日本周産期・新生児医学会雑誌 ( (一社)日本周産期・新生児医学会 )  60 ( Suppl.1 ) P336 - P336  2024.06

日本におけるCSTの現状,産婦人科CSTにおける役割

玉手 雅人, 松浦 基樹, 長尾 沙智子, 黒川 晶子, 秋元 太志, 幅田 周太朗, 齋藤 豪

産婦人科手術 ( (株)メジカルビュー社 )  ( 35 ) 121 - 121  2024.06

hinotoriを用いた婦人科ロボット支援手術

長尾 沙智子, 松浦 基樹, 黒川 晶子, 玉手 雅人, 秋元 太志, 諸井 明仁, 齋藤 豪

産婦人科手術 ( (株)メジカルビュー社 )  ( 35 ) 150 - 150  2024.06

vNOTESの可能性を広げる術式の研究

玉手 雅人, 松浦 基樹, 長尾 沙智子, 黒川 晶子, 諸井 明仁, 秋元 太志, 齋藤 豪

産婦人科手術 ( (株)メジカルビュー社 )  ( 35 ) 143 - 143  2024.06

vNOTESを導入する際の諸問題 vNOTES,船頭多くして船海に出るために

玉手 雅人, 松浦 基樹, 長尾 沙智子, 黒川 晶子, 諸井 明仁, 秋元 太志, 幅田 周太朗, 齋藤 豪

産婦人科手術 ( (株)メジカルビュー社 )  ( 35 ) 137 - 137  2024.06

日本におけるCSTの現状,産婦人科CSTにおける役割

玉手 雅人, 松浦 基樹, 長尾 沙智子, 黒川 晶子, 秋元 太志, 幅田 周太朗, 齋藤 豪

産婦人科手術 ( (株)メジカルビュー社 )  ( 35 ) 121 - 121  2024.06

vNOTESの可能性を広げる術式の研究

玉手 雅人, 松浦 基樹, 長尾 沙智子, 黒川 晶子, 諸井 明仁, 秋元 太志, 齋藤 豪

産婦人科手術 ( (株)メジカルビュー社 )  ( 35 ) 143 - 143  2024.06

vNOTESを導入する際の諸問題 vNOTES,船頭多くして船海に出るために

玉手 雅人, 松浦 基樹, 長尾 沙智子, 黒川 晶子, 諸井 明仁, 秋元 太志, 幅田 周太朗, 齋藤 豪

産婦人科手術 ( (株)メジカルビュー社 )  ( 35 ) 137 - 137  2024.06

hinotoriを用いた婦人科ロボット支援手術

長尾 沙智子, 松浦 基樹, 黒川 晶子, 玉手 雅人, 秋元 太志, 諸井 明仁, 齋藤 豪

産婦人科手術 ( (株)メジカルビュー社 )  ( 35 ) 150 - 150  2024.06

Early outcomes of three new robotic surgical systems in patients undergoing hysterectomy.

Motoki Matsuura, Sachiko Nagao, Shoko Kurokawa, Masato Tamate, Taishi Akimoto, Tsuyoshi Saito

Updates in surgery    2024.05  [International journal]

　View Summary

New robot models, such as hinotori™, da Vinci SP™, and Hugo™, have been introduced in Japan. This study examined the surgical outcomes of these models in patients from the initial stages of their introduction to the present day.We retrospectively reviewed 36 patients with endometrial cancer or benign gynecologic disease, who underwent robotic hysterectomy using da Vinci SP™, hinotori™, or Hugo™ between March 2023 and March 2024.Robotic hysterectomy was performed using hinotori™ in 10 patients, da Vinci SP™ in 16 patients, and Hugo™ in 10 patients. No significant differences were observed in the characteristics of the patients subjected to surgery using these models. The total operative time was 123.0 min (93-144 min) for hinotori™, 95.0 min (79-165 min) for da Vinci SP™, and 98.5 min (74-177 min) for Hugo™. The total operative time of hinotori™ was significantly longer than that of the other two models (p = 0.031). No differences were observed among the robot systems with respect to complications during or after surgery and the intensity of postoperative pain.Differences in the surgical time were noted depending on the model used. It has been proven that surgeons who are already proficient in performing robotic surgery with da Vinci Xi™ can safely perform surgeries with the new models.

DOI PubMed

Surgical Outcomes of da Vinci Xi™ and da Vinci SP™ for Early-Stage Endometrial Cancer in Patients Undergoing Hysterectomy.

Motoki Matsuura, Sachiko Nagao, Shoko Kurokawa, Masato Tamate, Taishi Akimoto, Tsuyoshi Saito

Journal of clinical medicine   13 ( 10 )  2024.05  [International journal]

　View Summary

Objectives: This study aimed to evaluate and compare the feasibility and outcomes of two robotic hysterectomy (da Vinci Xi™ vs. da Vinci SP™) systems without lymph node dissection in patients with early-stage endometrial cancer, and assess the postoperative recurrence rate and overall survival of patients. Methods: A retrospective review of 84 patients who underwent robotic hysterectomy for endometrial cancer (stage 1A) was conducted. Surgical procedures, patient characteristics, intraoperative measures, and postoperative outcomes were statistically analyzed. A single gynecologist performed all surgeries. Results: Patient characteristics, average age, and body mass index showed no significant differences between the two models. The total operative time was significantly shorter with da Vinci SP™. Recurrence was identified in only one patient operated on with da Vinci Xi™. All patients were alive during analysis, with a median overall survival of 38 and 9 months for da Vinci Xi™ and da Vinci SP™, respectively. Conclusions: Robotic hysterectomy without lymph node dissection appears to be a safe and effective approach for patients with early-stage endometrial cancer. The da Vinci SP offers the advantage of shorter operative times than the da Vinci Xi™. These findings support the consideration of robotic surgery as a viable option for selected patients.

DOI PubMed

Prediction of Mismatch Repair Status in Endometrial Cancer from Histological Slide Images Using Various Deep Learning-Based Algorithms.

Mina Umemoto, Tasuku Mariya, Yuta Nambu, Mai Nagata, Toshihiro Horimai, Shintaro Sugita, Takayuki Kanaseki, Yuka Takenaka, Shota Shinkai, Motoki Matsuura, Masahiro Iwasaki, Yoshihiko Hirohashi, Tadashi Hasegawa, Toshihiko Torigoe, Yuichi Fujino, Tsuyoshi Saito

Cancers   16 ( 10 )  2024.05  [International journal]

　View Summary

The application of deep learning algorithms to predict the molecular profiles of various cancers from digital images of hematoxylin and eosin (H&E)-stained slides has been reported in recent years, mainly for gastric and colon cancers. In this study, we investigated the potential use of H&E-stained endometrial cancer slide images to predict the associated mismatch repair (MMR) status. H&E-stained slide images were collected from 127 cases of the primary lesion of endometrial cancer. After digitization using a Nanozoomer virtual slide scanner (Hamamatsu Photonics), we segmented the scanned images into 5397 tiles of 512 × 512 pixels. The MMR proteins (PMS2, MSH6) were immunohistochemically stained, classified into MMR proficient/deficient, and annotated for each case and tile. We trained several neural networks, including convolutional and attention-based networks, using tiles annotated with the MMR status. Among the tested networks, ResNet50 exhibited the highest area under the receiver operating characteristic curve (AUROC) of 0.91 for predicting the MMR status. The constructed prediction algorithm may be applicable to other molecular profiles and useful for pre-screening before implementing other, more costly genetic profiling tests.

DOI PubMed

我々はこうしている～子宮頸癌手術におけるMISの工夫 我々が行う子宮頸癌に対する腹腔鏡下広汎子宮全摘術の工夫

松浦 基樹, 長尾 沙智子, 黒川 晶子, 玉手 雅人, 秋元 太志, 幅田 周太朗, 齋藤 豪

日本婦人科腫瘍学会雑誌 ( (公社)日本婦人科腫瘍学会 )  42 ( 2 ) 114 - 117  2024.04

自己採取および尿採取法による腟内HPV検査の患者の受け入れに関する検討

齋藤 豪, 松浦 基樹, 寺田 倫子, 玉手 雅人, 高田 優子, 西村 庸子, 山下 真祐子, 諸井 明仁

日本女性医学学会雑誌 ( (一社)日本女性医学学会 )  31 ( 3 ) 404 - 409  2024.04

　View Summary

子宮頸がん検診の受診率向上は喫緊の課題である.自己採取または尿採取腟内HPV検査は複数の介入研究で受診率向上に有効と報告されている.医師採取HPV検査と同等の感度があれば,受診率向上が課題であるわが国において有効な手段となりうる.われわれはこれまで自己採取・尿採取HPV検査の有効性について比較検討研究を行い報告してきたが,本研究ではよりよい自己採取法を目指し,検体採取対象者に対し検査の受け入れに関するアンケート調査を行った.年齢は20～50歳とし,CIN2以上の患者を100名以上含む計300名を対象に研究を行った.アンケート300人中281人が回答し,子宮頸がんについては「知っている」,あるいは,「なんとなく知っている」と答えた人が93.1%,頸がんが検診でがんになる前の段階で見つけることを「知っている」と答えた人は87.9%,頸がんの原因がHPVと言うウイルスの感染であることを「知っている」と答えた人の割合は93.3%といずれも高い割合であった.採取前に38.6%が不安ありと答えたが,すべての患者が採取を完遂し患者にとって受け入れられているものと考えた.頸がんに対する認知度は高いものの検診率の増加に結びついていないことが今後の課題であり,自己採取HPV検査も解決策の一つと考えられた.(著者抄録)

がん患者の妊孕性温存に関する情報提供の現状と課題

古来 愛香, 玉手 雅人, 馬場 剛, 長尾 沙智子, 小川 万梨絵, 森下 美幸, 久野 芳佳, 齋藤 豪

日本生殖医学会雑誌 ( (一社)日本生殖医学会 )  69 ( 1-2 ) 121 - 121  2024.04

子宮移植までのマイルストーン

玉手 雅人, 馬場 剛, 和田 渚, 古来 愛香, 幅田 周太朗, 小川 万梨絵, 松浦 基樹, 齋藤 豪

日本生殖医学会雑誌 ( (一社)日本生殖医学会 )  69 ( 1-2 ) 122 - 122  2024.04