研究者詳細 - 齋藤　豪

論文 - 齋藤　豪

分割表示 473 件中 201 - 220 件目／全件表示 >>

骨盤解剖学をCadaver Surgery Trainingで理解する混合研究の試み

玉手雅人, 松浦基樹, 阿部秀悦, 西村庸子, 常松梨沙, 寺田倫子, 真里谷奨, 岩崎雅宏, 金尾祐之, 齋藤豪

日本婦人科腫瘍学会雑誌 ( (公社)日本婦人科腫瘍学会 ) 39 ( 1 ) 311 - 311 2021年01月

Deep Learning(YOLOv3)を用いたリアルタイム婦人科細胞診断支援システム設計の試み

真里谷奨, 新開翔太, 松浦基樹, 郷久晴朗, 岩崎雅宏, 南部優太, 藤野雄一, 淺沼広子, 廣橋良彦, 鳥越俊彦, 齋藤豪

日本婦人科腫瘍学会雑誌 ( (公社)日本婦人科腫瘍学会 ) 39 ( 1 ) 278 - 278 2021年01月

ERα発現および非発現子宮体癌細胞株に於けるBAG3発現および増殖能との関連

阿部秀悦, 岩崎雅宏, 幅田周太朗, 真里谷奨, 小川万梨絵, 郷久晴朗, 齋藤豪

日本婦人科腫瘍学会雑誌 ( (公社)日本婦人科腫瘍学会 ) 39 ( 1 ) 285 - 285 2021年01月

Deep Learning(YOLOv3)を用いたリアルタイム婦人科細胞診断支援システム設計の試み

真里谷奨, 新開翔太, 松浦基樹, 郷久晴朗, 岩崎雅宏, 南部優太, 藤野雄一, 淺沼広子, 廣橋良彦, 鳥越俊彦, 齋藤豪

日本婦人科腫瘍学会雑誌 ( (公社)日本婦人科腫瘍学会 ) 39 ( 1 ) 278 - 278 2021年01月

ERα発現および非発現子宮体癌細胞株に於けるBAG3発現および増殖能との関連

阿部秀悦, 岩崎雅宏, 幅田周太朗, 真里谷奨, 小川万梨絵, 郷久晴朗, 齋藤豪

日本婦人科腫瘍学会雑誌 ( (公社)日本婦人科腫瘍学会 ) 39 ( 1 ) 285 - 285 2021年01月

骨盤解剖学をCadaver Surgery Trainingで理解する混合研究の試み

玉手雅人, 松浦基樹, 阿部秀悦, 西村庸子, 常松梨沙, 寺田倫子, 真里谷奨, 岩崎雅宏, 金尾祐之, 齋藤豪

日本婦人科腫瘍学会雑誌 ( (公社)日本婦人科腫瘍学会 ) 39 ( 1 ) 311 - 311 2021年01月

タイト結合分子Cluadin-2をターゲットにした子宮内膜癌治療のin vitroにおける検討

嶋田浩志, 岡田匡氷, 金野匠, 幸野貴之, 松浦基樹, 郷久晴朗, 岩崎雅宏, 小島隆, 齋藤豪

日本婦人科腫瘍学会雑誌 ( (公社)日本婦人科腫瘍学会 ) 39 ( 1 ) 285 - 285 2021年01月

腹腔鏡下子宮筋腫核出術後に発生したparasitic myomaの1例

岩崎雅宏, 真里谷奨, 阿部秀悦, 松浦基樹, 玉手雅人, 齋藤豪

日本産科婦人科内視鏡学会雑誌 ( (一社)日本産科婦人科内視鏡学会 ) 36 ( Suppl.I ) [O - 196] 2020年11月

Possibility of Targeting Claudin-2 in Therapy for Human Endometrioid Endometrial Carcinoma.

Tadahi Okada, Takumi Konno, Takayuki Kohno, Hiroshi Shimada, Kimihito Saito, Seiro Satohisa, Tsuyoshi Saito, Takashi Kojima

Reproductive sciences (Thousand Oaks, Calif.) 27 ( 11 ) 2092 - 2103 2020年11月 [国際誌]

Claudin-2 (CLDN-2) is a leaky-type tight junction protein, and its overexpression increases tumorigenesis of some types of cancer cells. In the present study, to examine the possibility of targeting CLDN-2 in the therapy for endometrioid endometrial adenocarcinoma, we investigated the regulation and role of CLDN-2 in endometriosis and endometrioid endometrial adenocarcinoma. In endometrioid endometrial adenocarcinoma tissues, marked upregulation of CLDN-2 was observed together with malignancy, while in endometriosis tissues, a change in the localization of CLDN-2 was observed. In cells of the endometrial adenocarcinoma cell line Sawano, which highly express CLDN-2, downregulation of CLDN-2 induced by the siRNA upregulated the epithelial barrier and inhibited cell migration. Furthermore, the downregulation of CLDN-2 affected the cell cycle and inhibited cell proliferation. In Sawano cells cultured with high-glucose medium, CLDN-2 expression was downregulated at the mRNA and protein levels. The high-glucose medium upregulated the epithelial barrier, cell proliferation, and migration, and inhibited cell invasion. The histone deacetylase (HDAC) inhibitor tricostatin A (TSA), which has antitumor effects, downregulated CLDN-2 expression, cell proliferation, invasion, and migration, and upregulated the epithelial barrier. The mitochondrial respiration level, an indicator of cancer metabolism, was downregulated by CLDN-2 knockdown and upregulated by the high-glucose condition. Taken together, these results indicated that overexpression of CLDN-2 closely contributed to the malignancy of endometrioid endometrial adenocarcinoma. Downregulation of CLDN-2 via the changes of the glucose concentration and treatment with HDAC inhibitors may be important in the therapy for endometrial cancer.

DOI PubMed

胎児期に診断に苦慮した先天性嚢胞状腺腫様形成異常に胸腔外肺分画症を合併した一例

染谷真行, 古来愛香, 新開翔太, 真里谷奨, 石岡伸一, 齋藤豪

超音波医学 ( (公社)日本超音波医学会 ) 47 ( Suppl. ) S418 - S418 2020年11月

若手医師に対する腹腔鏡手術の教育原点回帰、Cadaver Surgery Trainingを用いた教育

玉手雅人, 松浦基樹, 篠原由梨, 黒川晶子, 西村庸子, 磯山響子, 寺田倫子, 金尾祐之, 齋藤豪

日本産科婦人科内視鏡学会雑誌 ( (一社)日本産科婦人科内視鏡学会 ) 36 ( Suppl.I ) [SY1 - 4] 2020年11月

YOLOv3を用いた子宮頸部細胞診画像クラスタリング手法の提案

南部優太, 真里谷奨, 新開翔太, 淺沼広子, 廣橋良彦, 齋藤豪, 鳥越俊彦, 佐藤生馬, 藤野雄一

医療情報学連合大会論文集 ( (一社)日本医療情報学会 ) 40回 482 - 486 2020年11月

腹腔鏡下子宮全摘出術施行時に偶発的に副脾を認めた一例

長尾沙智子, 有元千紘, 山崎智子, 玉手雅人, 恐神博行, 高田さくら, 松浦基樹, 齋藤豪

日本産科婦人科内視鏡学会雑誌 ( (一社)日本産科婦人科内視鏡学会 ) 36 ( Suppl.I ) [O - 046] 2020年11月

腹腔鏡下子宮筋腫核出術後に発生したparasitic myomaの1例

岩崎雅宏, 真里谷奨, 阿部秀悦, 松浦基樹, 玉手雅人, 齋藤豪

日本産科婦人科内視鏡学会雑誌 ( (一社)日本産科婦人科内視鏡学会 ) 36 ( Suppl.I ) [O - 196] 2020年11月

深層学習を用いた子宮頸部細胞診人工知能診断支援システムの有用性について

真里谷奨, 南部優太, 淺沼広子, 廣橋良彦, 岩崎雅宏, 藤野雄一, 鳥越俊彦, 齋藤豪

日本癌学会総会記事 ( (一社)日本癌学会 ) 79回 OJ15 - 7 2020年10月

Association between cancer immunity and treatment results in uterine cervical cancer patients treated with radiotherapy

Masanori Someya, Takaaki Tsuchiya, Yuki Fukushima, Tomokazu Hasegawa, Yu Takada, Masakazu Hori, Katsutoshi Miura, Mio Kitagawa, Toshio Gocho, Yoshihiko Hirohashi, Toshihiko Torigoe, Masahiro Iwasaki, Motoki Matsuura, Tsuyoshi Saito, Koh-ichi Sakata

Japanese Journal of Clinical Oncology ( Oxford University Press (OUP) ) 2020年08月

Abstract Objective To evaluate proteins related to tumor immune response and treatment outcome from radiotherapy for uterine cervical cancer patients. Methods We performed a retrospective immunohistochemical staining of 81 patients with uterine cervical cancer who underwent definitive radiotherapy. We examined the expression of programmed death ligand 1, human leukocyte antigen class I, tumor-infiltrating CD8+, and forkhead box P3+ (FoxP3+) T cells in tumor tissues. Results In biopsy specimen, patients with a higher number of CD8+ T cells and FoxP3+ T cells had a better disease-specific survival than patients with a lower number of CD8+ T cells and FoxP3+ cells (P = 0.018 and P = 0.009). Multivariate analysis showed that equivalent dose in 2 Gy fractions (EQD2) of the minimum dose to 90% of the high-risk clinical target volume, FoxP3+ T cells and expression of human leukocyte antigen class I were significant prognostic factors. When the EQD2 is 70 Gy or more, a higher local control rate is obtained regardless of the number of CD8- or FoxP3-positive cells. When EQD2 is <70 Gy, the number of CD8-positive cells has a significant impact on treatment outcome: the recurrence rate (local recurrence rate + distant metastasis rate) was 46.2% in the group with a CD8 value of 230 or higher, whereas the recurrence rate was 75.7% in the group with a CD8 value of less than 230. Conclusion The combination of CD8 or FoxP3 with EQD2 can be potentially useful to predict the treatment results of radiotherapy for cervical cancer, leading to individualized optimal selection of treatment for cervical cancer.

DOI

Aldolase A promotes epithelial-mesenchymal transition to increase malignant potentials of cervical adenocarcinoma.

Yuki Saito, Akira Takasawa, Kumi Takasawa, Tomoyuki Aoyama, Taishi Akimoto, Misaki Ota, Kazufumi Magara, Masaki Murata, Yoshihiko Hirohashi, Tadashi Hasegawa, Norimasa Sawada, Tsuyoshi Saito, Makoto Osanai

Cancer science 111 ( 8 ) 3071 - 3081 2020年08月 [国際誌]

Recent studies have revealed that metabolic reprogramming is closely associated with epithelial-mesenchymal transition (EMT) during cancer progression. Aldolase A (ALDOA) is a key glycolytic enzyme that is highly expressed in several types of cancer. In this study, we found that ALDOA is highly expressed in uterine cervical adenocarcinoma and that high ALDOA expression promotes EMT to increase malignant potentials, such as metastasis and invasiveness, in cervical adenocarcinoma cells. In human surgical specimens, ALDOA was highly expressed in cervical adenocarcinoma and high ALDOA expression was correlated with lymph node metastasis, lymphovascular infiltration, and short overall survival. Suppression of ALDOA expression significantly reduced cell growth, migration, and invasiveness of cervical cancer cells. Aldolase A expression was partially regulated by hypoxia-inducible factor-1α (HIF-1α). Shotgun proteome analysis revealed that cell-cell adhesion-related proteins were significantly increased in ALDOA-overexpressing cells. Interestingly, overexpression of ALDOA caused severe morphological changes, including a cuboidal-to-spindle shape shift and reduced microvilli formation, coincident with modulation of the expression of typical EMT-related proteins. Overexpression of ALDOA increased migration and invasion in vitro. Furthermore, overexpression of ALDOA induced HIF-1α, suggesting a positive feedback loop between ALDOA and HIF-1α. In conclusion, ALDOA is overexpressed in cervical adenocarcinoma and contributes to malignant potentials of tumor cells through modulation of HIF-1α signaling. The feedback loop between ALDOA and HIF-1α could become a therapeutic target to improve the prognosis of this malignancy.

DOI PubMed

Association between radiotherapy-induced alteration of programmed death ligand 1 and survival in patients with uterine cervical cancer undergoing preoperative radiotherapy.

Takaaki Tsuchiya, Masanori Someya, Yu Takada, Tomokazu Hasegawa, Mio Kitagawa, Yuki Fukushima, Toshio Gocho, Masakazu Hori, Kensei Nakata, Yoshihiko Hirohashi, Toshihiko Torigoe, Tsuyoshi Saito, Koh-Ichi Sakata

Strahlentherapie und Onkologie : Organ der Deutschen Rontgengesellschaft ... [et al] 196 ( 8 ) 725 - 735 2020年08月 [国際誌]

PURPOSE: To evaluate radiotherapy-induced changes in the expression of programmed death ligand 1 (PD-L1), programmed death 1 (PD-1), and human leukocyte antigen class I (HLA-1) in patients with uterine cervical cancer, as well as infiltration of CD8+ and Forkhead box P3+ (FoxP3+) T lymphocytes into tumor tissue and the prognostic value of these parameters. MATERIALS AND METHODS: We performed immunohistochemical analysis of pre-radiotherapy biopsies and corresponding post-radiotherapy resected tissues in 104 uterine cervical cancer patients undergoing preoperative chemoradiotherapy or radiotherapy alone. We scored the expression of various proteins to distinguish positive from negative samples. RESULTS: PD-L1-expressing tumor cells (PD-L1 TC) increased significantly after chemoradiotherapy (p = 0.043). CD8+ T cell infiltration (p = 0.002) and FoxP3+ T cell infiltration (p = 0.003) decreased significantly after chemoradiotherapy. Expression of PD‑1, PD-L1-expressing immune cells (PD-L1 IC), and HLA‑1 did not change after chemoradiotherapy. In biopsy specimens obtained before chemoradiotherapy or radiotherapy, greater infiltration of CD8+ T cells (p = 0.001) and FoxP3+ T cells (p = 0.003) were significant predictors of better overall survival (OS). In surgical specimens obtained after chemoradiotherapy or radiotherapy, greater infiltration of PD-L1 TC was the only significant predictor of better OS (p < 0.001) and was related to a significantly lower probability of out-of-field recurrence (p = 0.005). CONCLUSION: Chemoradiotherapy induced an immunologic shift that increased PD-L1 TC. Chemoradiotherapy has immunological effects that can influence the results of treatment for uterine cervical cancer.

DOI PubMed

ASPP2 suppression promotes malignancy via LSR and YAP in human endometrial cancer.

Takumi Konno, Takayuki Kohno, Tadahi Okada, Hiroshi Shimada, Seiro Satohisa, Shin Kikuchi, Tsuyoshi Saito, Takashi Kojima

Histochemistry and cell biology 154 ( 2 ) 197 - 213 2020年08月 [国際誌]

Apoptosis-stimulating p53 protein 2 (ASPP2) is an apoptosis inducer that acts via binding with p53 and epithelial polarity molecule PAR3. Lipolysis-stimulated lipoprotein receptor (LSR) is an important molecule at tricellular contacts, and loss of LSR promotes cell migration and invasion via Yes-associated protein (YAP) in human endometrial cancer cells. In the present study, to find how ASPP2 suppression promotes malignancy in human endometrial cancer, we investigated its mechanisms including the relationship with LSR. In endometriosis and endometrial cancers (G1 and G2), ASPP2 was observed as well as PAR3 and LSR in the subapical region. ASPP2 decreased in G3 endometrial cancer compared to G1. In human endometrial cancer cell line Sawano, ASPP2 was colocalized with LSR and tricellulin at tricellular contacts and binding to PAR3, LSR, and tricellulin in the confluent state. ASPP2 suppression promoted cell migration and invasion, decreased LSR expression, and induced expression of phosphorylated YAP, claudin-1, -4, and -7 as effectively as the loss of LSR. Knockdown of YAP prevented the upregulation of pYAP, cell migration and invasion induced by the ASPP2 suppression. Treatment with a specific antibody against ASPP2 downregulated ASPP2 and LSR, affected F-actin at tricellular contacts, upregulated expression of pYAP and claudin-1, and induced cell migration and invasion via YAP. In normal human endometrial epithelial cells, ASPP2 was in part colocalized with LSR at tricellular contacts and knockdown of ASPP2 or LSR induced expression of claudin-1 and claudin-4. ASPP2 suppression promoted cell invasion and migration via LSR and YAP in human endometrial cancer cells.

DOI PubMed

ロボット手術におけるPeNのコツ

松浦基樹, 西村庸子, 黒川晶子, 玉手雅人, 磯山響子, 寺田倫子, 郷久晴朗, 岩崎雅宏, 齋藤豪

産婦人科手術 ( (株)メジカルビュー社 ) ( 31 ) 155 - 155 2020年08月

　前のページ - 次のページ　

<齋藤 豪>

論文 - 齋藤 豪

<齋藤　豪>

論文 - 齋藤　豪