論文 - 齋藤　豪

Cell-free RNA発現プロファイルを根拠とした新規無侵襲着床前胚染色体異数性検査アルゴリズムの構築について

真里谷 奨, 齋藤 豪, 倉橋 浩樹

日本産科婦人科学会雑誌 ( (公社)日本産科婦人科学会 )  75 ( 臨増 ) S - 430  2023年02月

中心核ミオパチーの児出産を契機に発見された1型リアノジン受容体(RYR1)遺伝子のバリアントを有する妊婦の一例

古来 愛香, 石岡 伸一, 新開 翔太, 真里谷 奨, 常松 梨紗, 染谷 真行, 小川 万梨絵, 森下 美幸, 馬場 剛, 齋藤 豪

日本産科婦人科学会雑誌 ( (公社)日本産科婦人科学会 )  75 ( 臨増 ) S - 459  2023年02月

深層学習を用いたHE染色からの子宮内膜癌分子生物学的プロファイル推定の適否に関する検討

梅本 美菜, 真里谷 奨, 新開 翔太, 松浦 基樹, 岩崎 雅宏, 齋藤 豪

日本産科婦人科学会雑誌 ( (公社)日本産科婦人科学会 )  75 ( 臨増 ) S - 260  2023年02月

深層学習を用いたHE染色からの子宮内膜癌分子生物学的プロファイル推定の適否に関する検討

梅本 美菜, 真里谷 奨, 新開 翔太, 松浦 基樹, 岩崎 雅宏, 齋藤 豪

日本産科婦人科学会雑誌 ( (公社)日本産科婦人科学会 )  75 ( 臨増 ) S - 260  2023年02月

Cell-free RNA発現プロファイルを根拠とした新規無侵襲着床前胚染色体異数性検査アルゴリズムの構築について

真里谷 奨, 齋藤 豪, 倉橋 浩樹

日本産科婦人科学会雑誌 ( (公社)日本産科婦人科学会 )  75 ( 臨増 ) S - 430  2023年02月

Letrozole increases preantral follicle growth and decreases estradiol production without impairing follicle survival.

Fukiko Kasuga-Yamashita, Tsuyoshi Baba, Sachiko Nagao, Yuya Fujibe, Miyuki Morishita, Yoshika Kuno, Tasuku Mariya, Hiroyuki Honnma, Toshiaki Endo, Tamotsu Kiya, Tsuyoshi Saito

Journal of ovarian research   15 ( 1 ) 136 - 136  2022年12月  [国際誌]

　概要を見る

BACKGROUND: Letrozole has been reported to be effective in treating anovulation, preventing ovarian hyperstimulation syndrome (OHSS), and retrieving oocytes in breast cancer patients. However, the role and mechanism of letrozole in follicular development remain unclear. RESULTS: We treated mouse preantral follicles with various treatments; we found no significant difference in follicle survival rates in the letrozole (LET) group compared with the control group, but the average diameter of follicles in the LET group tended to be larger (CTRL vs. LET 30, p = 0.064; CTRL vs. LET 100, p = 0.025). The estradiol concentrations in culture media of the LET group were significantly lower than those observed in the control group (CTRL vs. LET 30, p = 0.038; CTRL vs. LET 100, p = 0.025). We further found a marked increase in follicle-stimulating hormone receptor (FSHR) gene expression in response to letrozole treatment (CTRL vs. LET 30, p = 0.075; CTRL vs. LET 100, p = 0.034). This result suggested that increased FSHR expression promotes follicle development. Letrozole inhibited aromatase activity, but the effect was limited. Letrozole did not significantly reduce vascular endothelial growth factor (VEGF) gene expression. CONCLUSIONS: Letrozole may promote follicle development by increasing the expression of FSHR. Letrozole may be useful for fertility preservation of patients with estrogen-dependent cancers such as breast cancer and various other cancers. Whether letrozole has a direct effect in reducing OHSS requires further investigation.

DOI PubMed

vNOTESによる膜解剖理論に基づいた直腸周囲展開

足立 岳貴, 松浦 基樹, 黒川 晶子, 西村 庸子, 玉手 雅人, 秋元 太志, 齋藤 豪

日本内視鏡外科学会雑誌 ( (一社)日本内視鏡外科学会 )  27 ( 7 ) 599 - 599  2022年12月

A mixed-method evaluation of cadaver surgery training for gynecologic oncology.

Masato Tamate, Motoki Matsuura, Hiroyuki Kanao, Tsuyoshi Saito

The journal of obstetrics and gynaecology research   48 ( 12 ) 3252 - 3261  2022年12月  [国際誌]

　概要を見る

AIM: Traditional surgical training techniques with using patients, and new advances with live animals, artificial models, and simulation methods have several shortcomings. There are a few reports on the usefulness of cadaver surgery training (CST) in gynecology. Herein, we used a mixed-method evaluation to qualitatively and quantitatively explore the educational efficacy of CST by conducting CST programs at the Sapporo Medical University, Japan. METHODS: In 2020, we conducted two CST programs with 45 participants-13 residents, 8 specialists recognized by Japan Society of Obstetrics and Gynecology, 23 certified endoscopists recognized by Japan Society of Gynecology and Obstetrics Endoscopy and Minimally Invasive Therapy, 15 specialists recognized by Japan Society of Gynecologic Oncology, and 14 certified endoscopists cum oncology. Three participants observed the procedure virtually and 42 were physically present. Laparoscopic radical hysterectomy and pelvic exenteration were performed on five Thiel-embalmed cadavers. Participants were asked to complete pre- and post-training surveys that included qualitative questions, concerning the purpose and cost of CST, and quantitative questions, testing anatomical knowledge. RESULTS: We observed that the rate of score improvement to the quantitative questions increased from 58.6% pre-CST to 75.6% post-CST. Furthermore, oncology specialists and those who performed more surgeries and faced more complications during surgeries recorded high percentage of correct answers. Multiple regression analysis statistically confirmed these results. CONCLUSIONS: This study confirmed the educational efficacy of CST.

DOI PubMed

Predictive value of an exosomal microRNA-based signature for tumor immunity in cervical cancer patients treated with chemoradiotherapy.

Masanori Someya, Tomokazu Hasegawa, Takaaki Tsuchiya, Mio Kitagawa, Yuki Fukushima, Toshio Gocho, Shoh Mafune, Yutaro Ikeuchi, Yoh Kozuka, Masashi Idogawa, Yoshihiko Hirohashi, Toshihiko Torigoe, Masahiro Iwasaki, Motoki Matsuura, Tsuyoshi Saito, Koh-Ichi Sakata

Medical molecular morphology   56 ( 1 ) 38 - 45  2022年11月  [国内誌]

　概要を見る

Resistance of cervical cancer to radiotherapy with concurrent chemotherapy (CCRT) results in a poor prognosis. To identify new biomarkers for predicting the treatment response and prognosis, we explored exosomal microRNA (miRNA) expression signatures associated with the outcome of cervical cancer patients treated with CCRT. Exosomes were isolated from the plasma of 45 patients prior to CCRT during 2014-2020, and miRNA analysis was performed by next-generation sequencing. At a median follow-up of 38 months, 26 patients were recurrence free, 15 patients had died of the disease, and 4 patients received salvage chemotherapy due to distant metastasis. Of the 2522 miRNAs detected, 9 (miR-148a-5p, 1915-3p, 3960, 183-5p, 196b-5p, 200c-3p, 182-5p, 374a-5p, and 431-5p) showed differential expression between the recurrence-free and recurrence groups. Patients were divided into high- and low-risk groups according to the cutoff of the miRNAs-based risk score calculated from respective expression levels. The high-risk group had significantly worse disease-specific survival than the low-risk group (p < 0.001). In addition, miR-374a-5p and miR-431-5p expression showed a weak inverse correlation with tumor-infiltrating CD8+ and FOXP3+ T cells, suggesting a potential inhibitory effect on CCRT by suppressing tumor immunity. This miRNA signature could improve non-invasive monitoring and personalized treatment for cervical cancer.

DOI PubMed

子宮移植/妊娠中免疫抑制療法/胎児免疫 Thiel法固定献体における子宮移植トレーニング

玉手 雅人, 松浦 基樹, 木須 伊織, 齋藤 豪

Organ Biology ( (一社)日本臓器保存生物医学会 )  29 ( 3 ) 86 - 86  2022年11月

Human papillomavirus testing and cytology using physician-collected uterine cervical samples vs. self-collected vaginal samples and urine samples.

Noriko Terada, Motoki Matsuura, Shoko Kurokawa, Yoko Nishimura, Masato Tamate, Kyoko Isoyama, Satoko Yamazaki, Hiroshi Shimada, Masahiro Suzuki, Yuta Tabuchi, Mizue Teramoto, Tsuyoshi Saito

International journal of clinical oncology   27 ( 11 ) 1742 - 1749  2022年11月  [国内誌]

　概要を見る

BACKGROUND: Human papillomavirus (HPV) testing using self-collected vaginal samples and urine samples is convenient and effective for improving the screening rate. But, to serve as an alternative cervical cancer screening technique, such tests must offer sensitivity equivalent to the HPV testing of physician-collected cervical samples. To examine the effectiveness of HPV testing using self-collected samples and urine samples, we compared the results of HPV testing using these samples with those of HPV testing using physician-collected samples and cytological examinations. METHODS: The study population included 300 women (age: 20-50 years) with abnormal cervical cytology. The results of HPV testing using self-collected samples and urine samples and physician-collected samples and cervical cytology were compared. RESULTS: For all HPV types, the κ-value was 0.773 for physician- and self-collected samples and 0.575 for physician-collected and urine samples. The κ-value for HPV type 16-positive samples was 0.988 for physician- and self-collected samples and 0.896 for physician-collected and urine samples. The κ-value for HPV type 18-positive samples was 0.856 for physician- and self-collected samples and 0.831 for physician-collected and urine samples. For other HPV types, the value was 0.809 for physician- and self-collected samples and 0.617 for physician-collected and urine samples. CONCLUSIONS: The obtained results were consistent between physician- and self-collected samples as well as between physician-collected and urine samples. Considering that the agreement rate was particularly high for the high-risk HPV types 16 and 18, HPV testing using physician-collected samples, self-collected samples, and urine samples was equally effective for the types with high carcinogenicity.

DOI PubMed

子宮頸がん検診未受診者対策としての自己採取HPV検査の有効性評価

松浦 基樹, 寺田 倫子, 西村 庸子, 玉手 雅人, 長尾 沙智子, 秋元 太志, 幅田 周太朗, 岩崎 雅宏, 齋藤 豪

日本臨床細胞学会雑誌 ( (公社)日本臨床細胞学会 )  61 ( Suppl.2 ) 477 - 477  2022年10月

子宮悪性軟部腫瘍に対する新規薬剤が効果を認めた症例の検討

萬 和馬, 松浦 基樹, 足立 岳貴, 有元 千紘, 黒川 晶子, 西村 庸子, 玉手 雅人, 秋元 太志, 幅田 周太朗, 岩崎 雅宏, 齋藤 豪

日本癌治療学会学術集会抄録集 ( (一社)日本癌治療学会 )  60回   O46 - 2  2022年10月

子宮動脈塞栓術併用の子宮鏡下切除術において子宮内バルーニングが有用であったRPOCの一例

南 怜毅, 長尾 沙智子, 木嶋 紗弓, 小川 栞, 山崎 智子, 玉手 雅人, 松浦 基樹, 齋藤 豪

北日本産科婦人科学会総会・学術講演会プログラム・抄録集 ( 東北連合産科婦人科学会・北日本産科婦人科学会 )  69回   64 - 64  2022年10月

細胞診におけるAIの活用 子宮頸部細胞診におけるAIの活用

新開 翔太, 真里谷 奨, 梅本 美菜, 南部 優太, 永田 舞, 淺沼 広子, 上野 太郎, 廣橋 良彦, 鳥越 俊彦, 佐藤 生馬, 藤野 雄一, 齋藤 豪

日本臨床細胞学会雑誌 ( (公社)日本臨床細胞学会 )  61 ( Suppl.2 ) 447 - 447  2022年10月

子宮動脈塞栓術併用の子宮鏡下切除術において子宮内バルーニングが有用であったRPOCの一例

南 怜毅, 長尾 沙智子, 木嶋 紗弓, 小川 栞, 山崎 智子, 玉手 雅人, 松浦 基樹, 齋藤 豪

北日本産科婦人科学会総会・学術講演会プログラム・抄録集 ( 東北連合産科婦人科学会・北日本産科婦人科学会 )  69回   64 - 64  2022年10月

巨大嚢胞化し、子宮留膿腫を繰り返した帝王切開瘢痕部症候群の1例

仁木 舜一, 嶋田 浩志, 玉手 雅人, 杉山 芽, 今 沙織, 鈴木 美紀, 松浦 基樹, 齋藤 豪

北日本産科婦人科学会総会・学術講演会プログラム・抄録集 ( 東北連合産科婦人科学会・北日本産科婦人科学会 )  69回   112 - 112  2022年10月

帝王切開創のケロイド予防とその修復の取り組み

杉山 芽, 嶋田 浩志, 玉手 雅人, 仁木 舜一, 今 沙織, 鈴木 美紀, 石岡 伸一, 斎藤 豪

北日本産科婦人科学会総会・学術講演会プログラム・抄録集 ( 東北連合産科婦人科学会・北日本産科婦人科学会 )  69回   66 - 66  2022年10月

巨大嚢胞化し、子宮留膿腫を繰り返した帝王切開瘢痕部症候群の1例

仁木 舜一, 嶋田 浩志, 玉手 雅人, 杉山 芽, 今 沙織, 鈴木 美紀, 松浦 基樹, 齋藤 豪

北日本産科婦人科学会総会・学術講演会プログラム・抄録集 ( 東北連合産科婦人科学会・北日本産科婦人科学会 )  69回   112 - 112  2022年10月

Self-administration of gender-affirming hormones and supratherapeutic dosing are relatively common in Japanese transgender women.

Tsuyoshi Baba, Toshiaki Endo, Utako Ikeda, Hiroshi Ikeda, Koji Ichihara, Naoya Masumori, Tsuyoshi Saito

The journal of obstetrics and gynaecology research   48 ( 8 ) 2208 - 2213  2022年08月  [国際誌]

　概要を見る

AIM: This study was performed to determine the proportion of transgender women with self-adjusted hormone administration and excess dosing. METHODS: The medical records of 87 transgender women who visited our gender clinic from 2010 through 2019 were reviewed. The complete blood count and serum concentrations of D-dimer, gonadotropins, and sex steroids were compared between transgender women who were self-administering gender-affirming hormones and women not using such hormones. RESULTS: Fifty-eight of 87 (66.7%) transgender women had contravened the guideline and self-adjusted their hormone administration. The hormonal data of one woman with hypopituitarism were eliminated from the analyses. The serum gonadotropin and testosterone levels were significantly lower in the self-administration group than in the hormone-naïve group. Gonadotropin levels below the lower limit of normal were found in 32/86 (37.2%) transgender women. The testosterone levels in six transgender women were not analyzed because these women had undergone sex reassignment surgery before visiting our hospital. Testosterone levels below the lower limit of normal men were found in 36/80 (45.0%) transgender women. Unexpectedly, 29/36 (80.6%) transgender women who were classified as having suppressed serum testosterone levels had testosterone levels of <0.6 ng/mL, which corresponds to the levels in cisgender women. The white blood cell count and hemoglobin concentration were significantly different between the groups. CONCLUSION: Self-initiated hormonal treatments seem to affect the serum concentrations of gonadotropin and sex steroids and the complete blood count. The prevalence of transgender women with self-adjusted use of gender-affirming hormones is high, and an excess dose of hormones occasionally occurs.

DOI PubMed