論文 - 齋藤　豪

Pioglitazone suppresses excessive follicular development in murine preantral follicles.

Sachiko Nagao, Tsuyoshi Baba, Yuya Fujibe, Sayaka Adachi, Keiko Ikeda, Miyuki Morishita, Yoshika Kuno, Hiroyuki Honnma, Toshiaki Endo, Tamotsu Kiya, Tsuyoshi Saito

Journal of ovarian research   12 ( 1 ) 82 - 82  2019年08月  [国際誌]

　概要を見る

Polycystic ovary syndrome (PCOS) is an endocrine disease that is common in women in their reproductive period. Patients with this disease suffer from anovulation and hyperandrogenism. Ovulation induction with exogenous gonadotropin often causes ovarian hyperstimulation syndrome because many small antral follicles pause in their growth. Treatment with insulin sensitizers is reportedly effective for both anovulation associated with PCOS, and suppression of excessive follicular growth; however, the underlying mechanism of action remains unknown. Although pioglitazone is known as an insulin sensitizer, it also has a potent modulator of cell growth and apoptosis irrespective of insulin resistance. To clarify the effect of pioglitazone on follicular growth, we performed in vitro culture of murine preantral follicles. Secondary follicles (100-160 μm in diameter) isolated from 6-week-old ICR mice were individually cultured for 13 days. Culture conditions were as follows: 1) follicle-stimulating hormone (FSH; 33 mIU/mL; control), 2) FSH plus dihydrotestosterone (DHT; 500 ng/mL), 3) FSH plus pioglitazone (5 ng/mL), and 4) FSH plus DHT/pioglitazone. Survival rate and follicle diameter were evaluated, and concentrations of estradiol (E2) and vascular endothelial growth factor (VEGF) in culture media were measured. mRNA expression of various growth-promoting factors and Vegf within follicles were also assessed. Although no significant differences were observed with regard to survival rate, follicle diameters on day 13 were significantly different.Compared with the control group, the DHT group showed enhanced growth, while groups administered pioglitazone showed stagnation of the accelerated growth induced by DHT. Although DHT treatment enhanced the expression of bone morphogenetic protein 2 (Bmp2) mRNA, pioglitazone exposure suppressed induction of Bmp2 mRNA by DHT. Vegf mRNA and protein expression were also significantly reduced when pioglitazone was added to culture media containing DHT.Administration of pioglitazone negatively affected follicular growth and VEGF levels, which may suppress excessive follicular growth and prevent ovarian hyperstimulation syndrome.

DOI PubMed

重症子宮内膜症を有する不妊治療における手術とART介入のタイミング

森下 美幸, 長尾 沙智子, 新開 翔太, 久野 芳佳, 真里谷 奨, 馬場 剛, 遠藤 俊明, 齋藤 豪

日本産科婦人科内視鏡学会雑誌 ( (一社)日本産科婦人科内視鏡学会 )  35 ( Suppl.I ) 252 - 252  2019年08月

早期子宮体癌の診断で腹腔鏡下手術を施行した症例の腹腔細胞診に関する検討

西村 庸子, 石堂 茉泉, 神 未央奈, 長尾 沙智子, 玉手 雅人, 寺田 倫子, 松浦 基樹, 郷久 晴朗, 岩崎 雅宏, 齋藤 豪

日本産科婦人科内視鏡学会雑誌 ( (一社)日本産科婦人科内視鏡学会 )  35 ( Suppl.I ) 162 - 162  2019年08月

Fundamental Use of Surgical Energyを導入した手術教育

玉手 雅人, 松浦 基樹, 齋藤 豪

産婦人科手術 ( (株)メジカルビュー社 )  ( 30 ) 157 - 157  2019年08月

Cadaver Surgery Multi Angleな手術教育と研究

玉手 雅人, 松浦 基樹, 長尾 沙智子, 西村 庸子, 杉尾 明香, 竹中 慎, 明石 祐史, 林 茂徳, 金尾 祐之, 齋藤 豪

日本産科婦人科内視鏡学会雑誌 ( (一社)日本産科婦人科内視鏡学会 )  35 ( Suppl.I ) 119 - 119  2019年08月

術前に良性嚢胞腺腫と診断し、腹腔鏡手術後に卵巣癌と診断された1例

郷久 晴朗, 岩崎 雅宏, 鹿内 智史, 常松 梨紗, 阿部 秀悦, 小川 万梨絵, 西村 庸子, 玉手 雅人, 寺田 倫子, 松浦 基樹, 齋藤 豪

日本産科婦人科内視鏡学会雑誌 ( (一社)日本産科婦人科内視鏡学会 )  35 ( Suppl.I ) 265 - 265  2019年08月

産婦人科卒後後期研修医としてCadaverで学べること

神 未央奈, 玉手 雅人, 鹿内 智史, 伊野 善彦, 西村 庸子, 秋元 太志, 松浦 基樹, 齋藤 豪

日本産科婦人科内視鏡学会雑誌 ( (一社)日本産科婦人科内視鏡学会 )  35 ( Suppl.I ) 181 - 181  2019年08月

腹腔鏡下子宮体癌手術を行った95例の術前評価と術後病理組織診の比較検討

寺田 倫子, 松浦 基樹, 黒川 晶子, 西村 庸子, 玉手 雅人, 寺本 瑞絵, 齋藤 豪

日本産科婦人科内視鏡学会雑誌 ( (一社)日本産科婦人科内視鏡学会 )  35 ( Suppl.I ) 281 - 281  2019年08月

卵巣移動術後の内膜症性嚢胞に対し腹腔鏡で治療し得た1例

鹿内 智史, 玉手 雅人, 神 未央奈, 伊野 善彦, 鈴木 将裕, 西村 庸子, 松浦 基樹, 寺本 瑞絵, 齋藤 豪

日本産科婦人科内視鏡学会雑誌 ( (一社)日本産科婦人科内視鏡学会 )  35 ( Suppl.I ) 186 - 186  2019年08月

腹腔鏡下子宮筋腫核出術後に、ポート挿入部に腫瘍転移した1例

岩崎 雅宏, 郷久 晴朗, 小川 万梨絵, 阿部 秀悦, 松浦 基樹, 玉手 雅人, 西村 庸子, 常松 梨紗, 黒川 晶子, 齋藤 豪

日本産科婦人科内視鏡学会雑誌 ( (一社)日本産科婦人科内視鏡学会 )  35 ( Suppl.I ) 246 - 246  2019年08月

疲れにくい腹腔鏡手術 男女の身体的特性から

長尾 沙智子, 西村 庸子, 玉手 雅人, 松浦 基樹, 齋藤 豪

日本産科婦人科内視鏡学会雑誌 ( (一社)日本産科婦人科内視鏡学会 )  35 ( Suppl.I ) 117 - 117  2019年08月

男性均衡型相互転座保因者の体外受精胚の第一減数分裂で最も頻度の高い不均衡分離様式の予測は可能か?

遠藤 俊明, 馬場 剛, 鷹巣 祐子, 木谷 保, 尾崎 守, 加藤 武馬, 倉橋 浩樹, 斉藤 豪

日本遺伝カウンセリング学会誌 ( (一社)日本遺伝カウンセリング学会 )  40 ( 2 ) 84 - 84  2019年07月

Obstetrical prognosis of patients who underwent vaginal radical trachelectomy during pregnancy.

Mina Umemoto, Shinichi Ishioka, Yuko Mizugaki, Yuya Fujibe, Tasuku Mariya, Akari Kawamata, Masahito Mizuuchi, Miyuki Morishita, Tsuyoshi Baba, Tsuyoshi Saito

The journal of obstetrics and gynaecology research   45 ( 6 ) 1167 - 1172  2019年06月  [国際誌]

　概要を見る

AIM: Radical trachelectomy (RT) with pelvic lymphadenectomy has become an option for young patients with early invasive uterine cervical cancer who decide to maintain their fertility. However, this operative method entails a high risk for the following pregnancy due to its radicality. Therefore, RT for pregnant patients can be a challenge both for gynecologic oncologists and obstetricians. METHODS: We have performed vaginal RT for five pregnant patients with uterine cervical cancer stage 1B1 according to the method of Dargent et al. The operations were performed between 16 and 26 weeks of pregnancy, and the patients were followed up carefully according to the follow-up methods we reported previously. RESULTS: Vaginal RT was performed for five patients without any troubles. Four of the patients continued their pregnancies until almost 34 weeks or longer under our previously published follow-up schedule. The pregnancy of one patient was terminated at 26 weeks due to recurrence of the cancer. CONCLUSION: Expansion of vaginal RT for pregnant patients with uterine cervical cancer could be a practical option for pregnant patients with early invasive uterine cervical cancer.

DOI PubMed

リステリア菌血症により流産に至った1例

藤部 佑哉, 真里谷 奨, 嶋田 浩志, 山中 郁仁, 水内 将人, 寺本 瑞絵, 石岡 伸一, 齋藤 豪

日本周産期・新生児医学会雑誌 ( (一社)日本周産期・新生児医学会 )  55 ( 2 ) 530 - 530  2019年06月

子宮体部漿液性癌18例の検討

寺田 倫子, 松浦 基樹, 西村 庸子, 玉手 雅人, 郷久 晴朗, 岩崎 雅宏, 寺本 瑞絵, 齋藤 豪

日本臨床細胞学会雑誌 ( (公社)日本臨床細胞学会 )  58 ( Suppl.1 ) 276 - 276  2019年05月

子宮頸部原発大細胞神経内分泌癌の細胞像

寺本 瑞絵, 西村 庸子, 秋元 太志, 玉手 雅人, 磯山 響子, 小川 万梨絵, 寺田 倫子, 松浦 基樹, 郷久 晴朗, 岩崎 雅宏, 齊藤 豪

日本臨床細胞学会雑誌 ( (公社)日本臨床細胞学会 )  58 ( Suppl.1 ) 270 - 270  2019年05月

子宮内膜癌細胞におけるタイト結合分子claudin-2の関与

郷久 晴朗, 嶋田 浩志, 玉手 雅人, 秋元 太志, 西村 庸子, 寺田 倫子, 松浦 基樹, 岩崎 雅宏, 小島 隆, 齋藤 豪

日本臨床細胞学会雑誌 ( (公社)日本臨床細胞学会 )  58 ( Suppl.1 ) 320 - 320  2019年05月

子宮体癌におけるKRAS変異の意義

松浦 基樹, 西村 庸子, 玉手 雅人, 寺田 倫子, 郷久 晴朗, 岩崎 雅宏, 齋藤 豪

日本臨床細胞学会雑誌 ( (公社)日本臨床細胞学会 )  58 ( Suppl.1 ) 319 - 319  2019年05月

早期子宮体癌における腹腔細胞診に関する検討

西村 庸子, 松浦 基樹, 玉手 雅人, 寺田 倫子, 郷久 晴朗, 寺本 瑞絵, 岩崎 雅宏, 齋藤 豪

日本臨床細胞学会雑誌 ( (公社)日本臨床細胞学会 )  58 ( Suppl.1 ) 225 - 225  2019年05月

Androgen potentiates the expression of FSH receptor and supports preantral follicle development in mice.

Yuya Fujibe, Tsuyoshi Baba, Sachiko Nagao, Sayaka Adachi, Keiko Ikeda, Miyuki Morishita, Yoshika Kuno, Masahiro Suzuki, Masahito Mizuuchi, Hiroyuki Honnma, Toshiaki Endo, Tsuyoshi Saito

Journal of ovarian research   12 ( 1 ) 31 - 31  2019年04月  [国際誌]

　概要を見る

Hyperandrogenism is one of the cardinal symptoms in polycystic ovary syndrome and plays a key role in the pathogenesis of polycystic ovary syndrome. However, the precise effects and mechanisms of excess androgen during follicular development are still unclear. Here we investigated the effects of androgen on mouse follicle development in vitro. Androgen did not affect the growth of follicles smaller than 160-180 μm in the presence of follicle-stimulating hormone (FSH). However, in the presence of low FSH, androgen supported the growth of follicles larger than 160-180 μm, a size at which growing follicles acquire FSH-dependency. Androgen did not change the mRNA expression of various growth-promoting factors but did increase mRNA expression of the FSH receptor. We suggest that androgen has a positive impact on follicle development by augmentation of the actions of FSH. Therefore, FSH-responsive but FSH-independent follicles grow in the presence of a certain level of FSH or androgen, and androgen compensates for FSH deficiency in FSH-dependent follicles.

DOI PubMed