研究者詳細 - 齋藤　豪

論文 - 齋藤　豪

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A screening assistance system for cervical cytology of squamous cell atypia based on a two-step combined CNN algorithm with label smoothing.

Yuta Nambu, Tasuku Mariya, Shota Shinkai, Mina Umemoto, Hiroko Asanuma, Ikuma Sato, Yoshihiko Hirohashi, Toshihiko Torigoe, Yuichi Fujino, Tsuyoshi Saito

Cancer medicine 11 ( 2 ) 520 - 529 2022年01月 [国際誌]

BACKGROUND: Although many cervical cytology diagnostic support systems have been developed, it is challenging to classify overlapping cell clusters with a variety of patterns in the same way that humans do. In this study, we developed a fast and accurate system for the detection and classification of atypical cell clusters by using a two-step algorithm based on two different deep learning algorithms. METHODS: We created 919 cell images from liquid-based cervical cytological samples collected at Sapporo Medical University and annotated them based on the Bethesda system as a dataset for machine learning. Most of the images captured overlapping and crowded cells, and images were oversampled by digital processing. The detection system consists of two steps: (1) detection of atypical cells using You Only Look Once v4 (YOLOv4) and (2) classification of the detected cells using ResNeSt. A label smoothing algorithm was used for the dataset in the second classification step. This method annotates multiple correct classes from a single cell image with a smooth probability distribution. RESULTS: The first step, cell detection by YOLOv4, was able to detect all atypical cells above ASC-US without any observed false negatives. The detected cell images were then analyzed in the second step, cell classification by the ResNeSt algorithm, which exhibited average accuracy and F-measure values of 90.5% and 70.5%, respectively. The oversampling of the training image and label smoothing algorithm contributed to the improvement of the system's accuracy. CONCLUSION: This system combines two deep learning algorithms to enable accurate detection and classification of cell clusters based on the Bethesda system, which has been difficult to achieve in the past. We will conduct further research and development of this system as a platform for augmented reality microscopes for cytological diagnosis.

DOI PubMed

Twin pregnancy with untyped Ehlers-Danlos syndrome requiring prompt genetic testing: A case report.

Shiori Ogawa, Tasuku Mariya, Yuya Fujibe, Marie Ogawa, Keiko Ikeda, Miyako Mizukami, Yoshika Kuno, Aki Ishikawa, Shinichi Ishioka, Akihiro Sakurai, Tsuyoshi Saito

Case reports in women's health 33 e00384 2022年01月 [国際誌]

Ehlers-Danlos syndrome is a rare genetic disorder that presents with a variety of pathologies depending on the disease type. Among them, vascular Ehlers-Danlos syndrome requires extremely careful management as there have been many reports of fatal perinatal complications such as uterine rupture. Although hypermobile Ehlers-Danlos syndrome is less likely to cause fatal complications, symptoms such as arthralgia, hip dislocation, and depression may be seen throughout pregnancy. We report here a case of twin pregnancy in which Ehlers-Danlos syndrome was first suspected at 19 weeks of gestation. Vascular Ehlers-Danlos syndrome could not be ruled out based on family medical history, making it difficult to determine the perinatal management strategy. Prompt genetic testing did however rule out the vascular type and the patient was diagnosed with hypermobile Ehlers-Danlos syndrome from the clinical symptoms, enabling us to manage the pregnancy safely until 34 weeks of gestation.

DOI PubMed

後腟円蓋より発生する腟腫瘍に対し腹腔鏡下手術を行い、表在性筋線維芽細胞腫と判明した1例

鹿内智史, 松浦基樹, 曽山浩明, 和田渚, 梅本美菜, 三浦一郎, 齋藤豪

日本産科婦人科内視鏡学会雑誌 ( (一社)日本産科婦人科内視鏡学会 ) 38 ( 2 ) 230 - 234 2022年

Case of superficial myofibroblastoma protruding from the posterior fornix treated using laparoscopic surgery

Satoshi Shikanai, Motoki Matsuura, Hiroaki Soyama, Nagisa Wada, Mina Umemoto, Ichiro Miura, Tsuyoshi Saito

JAPANESE JOURNAL OF GYNECOLOGIC AND OBSTETRIC ENDOSCOPY ( Japan Society of Gynecologic and Obstetric Endoscopy and Minimally Invasive Therapy ) 38 ( 2 ) 230 - 234 2022年

DOI

Comparative Evaluation of Preoperative Imaging and Postoperative Histopathology in 108 Patients Who Underwent Laparoscopic- or Robot-Assisted Surgery for Endometrial Cancer.

Noriko Terada, Motoki Matsuura, Shoko Kurokawa, Mizue Teramoto, Masamitsu Hanakenaka, Tsuyoshi Saito

Gynecology and minimally invasive therapy 11 ( 4 ) 203 - 208 2022年 [国際誌]

OBJECTIVES: The aim of this study was to compare preoperative imaging evaluation results and postoperative histopathology examinations of patients who underwent laparoscopic- or robot-assisted surgery for suspected Stage IA endometrial carcinoma. MATERIALS AND METHODS: There were 298 patients diagnosed with endometrial cancer at our institution between April 2014 and March 2019. Preoperative imaging evaluation based on magnetic resonance imaging (MRI) and computed tomography (CT) revealed 180 patients suspected of having Stage IA disease. Of these, 72 underwent open surgery, 12 underwent robot-assisted surgery, and 96 underwent laparoscopic surgery. In this study, preoperative imaging evaluation results and postoperative histopathology examinations of 108 patients who underwent laparoscopic- or robot-assisted surgery were retrospectively compared. RESULTS: Of the 108 patients, who underwent laparoscopic- or robot-assisted surgery for suspected Stage IA disease, 98 had Stage IA, two had Stage IB, and four had Stages II and III disease. The sensitivity of imaging evaluations based on preoperative MRI and CT was 90.7%. CONCLUSION: Utilizing preoperative MRI and CT imaging resulted in a high rate of successful diagnosis of Stage IA endometrial carcinoma.

DOI PubMed

The Roles of Tricellular Tight Junction Protein Angulin-1/Lipolysis-Stimulated Lipoprotein Receptor (LSR) in Endometriosis and Endometrioid-Endometrial Carcinoma.

Hiroshi Shimada, Takayuki Kohno, Takumi Konno, Tadahi Okada, Kimihito Saito, Yuma Shindo, Shin Kikuchi, Mitsuhiro Tsujiwaki, Marie Ogawa, Motoki Matsuura, Tsuyoshi Saito, Takashi Kojima

Cancers 13 ( 24 ) 2021年12月 [国際誌]

Tight junction proteins play roles beyond permeability barriers functions and control cell proliferation and differentiation. The relation between tight junctions and the signal transduction pathways affects cell growth, invasion and migration. Abnormality of tight junction proteins closely contributes to epithelial mesenchymal transition (EMT) and malignancy of various cancers. Angulin-1/lipolysis-stimulated lipoprotein receptor (LSR) forms tricellular contacts that has a barrier function. Downregulation of angulin-1/LSR correlates with the malignancy in various cancers, including endometrioid-endometrial carcinoma (EEC). These alterations have been shown to link to not only multiple signaling pathways such as Hippo/YAP, HDAC, AMPK, but also cell metabolism in ECC cell line Sawano. Moreover, loss of angulin-1/LSR upregulates claudin-1, and loss of apoptosis stimulating p53 protein 2 (ASPP2) downregulates angulin-1/LSR. Angulin-1/LSR and ASPP2 concentrate at both midbody and centrosome in cytokinesis. In EEC tissues, angulin-1/LSR and ASPP2 are reduced and claudin-2 is overexpressed during malignancy, while in the tissues of endometriosis changes in localization of angulin-1/LSR and claudin-2 are seen. This review highlights how downregulation of angulin-1/LSR promotes development of endometriosis and EEC and discusses about the roles of angulin-1/LSR and its related proteins, including claudins and ASPP2.

DOI PubMed

Next-generation sequencing of 16S rRNA for identification of invasive bacterial pathogens in a formalin-fixed paraffin-embedded placental specimen: a case report of perinatal fulminant Streptococcus pyogenes infection

Tasuku Mariya, Toyotaka Sato, Yuya Fujibe, Manami Ishido, Hiroshi Shimada, Terufumi Kubo, Yoko Nagai, Wataru Arai, Suguru E. Tanaka, Kyota Ashikawa, Yoshiyuki Sakuraba, Shinichi Ishioka, Shin-ichi Yokota, Tsuyoshi Saito

Medical Molecular Morphology ( Springer Science and Business Media LLC ) 54 ( 4 ) 374 - 379 2021年12月 [査読有り] [国内誌]

Intrauterine infection is one of the most important causes of maternal death. In perinatal emergency, we often miss an opportunity to obtain culture specimens. In this study, we tried to examine whether we investigated whether bacteria causing infection can be detected from a formalin-fixed paraffin-embedded (FFPE) placental specimen. We examined the placenta from a maternal invasive infection that resulted in infectious abortion at 18 weeks of gestation. The case was diagnosed by acute fever and abdominal pain, and the patient was cured after 3 weeks of intensive antimicrobial treatment. Four Streptococcus pyogenes strains were isolated from vaginal fluid and blood cultures of the patient. All of the strain types were emm1/ST28. We amplified the V1-V2 region of 16S rRNA from an FFPE placental specimen and sequencing was performed using a next-generation sequencer (NGS). Taxonomic analysis was then performed for sequenced data. We succeeded in detecting causative pathogens from the FFPE placenta: 69.1% of the predominantly identified bacteria were S. pyogenes and other small populations of bacteria were detected. Our results revealed the utility of NGS for 16S rRNA analysis of an FFPE placenta. This method may reveal previous perinatal invasive infections of unknown origin retrospectively.

DOI PubMed

子宮頸がんに対する鏡視下広汎子宮全摘術の現状と課題-LACC studyから2年- 鏡視下広汎子宮全摘術の一隅を照らすCST教育

玉手雅人, 松浦基樹, 西村庸子, 常松梨紗, 幅田周太朗, 金尾祐之, 齋藤豪

日本内視鏡外科学会雑誌 ( (一社)日本内視鏡外科学会 ) 26 ( 7 ) WS9 - 7 2021年12月

マイクロバイオーム最前線-ヒト常在菌叢と各種疾患との関連- 腟内および子宮内細菌叢Dysbiosisへの真なるアプローチとは?

真里谷奨, 島野敏司, 遠藤俊明, 和田真一郎, 山田秀人, 藤井美穂, 水無瀬昂, 桜庭喜行, 鳥越俊彦, 齋藤豪

日本臨床微生物学会雑誌 ( (一社)日本臨床微生物学会 ) 32 ( Suppl.1 ) 164 - 164 2021年12月

Radical Vaginal Trachelectomy.

Tsuyoshi Saito, Motoki Matsuura, Masato Tamate, Masahiro Iwasaki, Tasuku Mariya

Surgery journal (New York, N.Y.) 7 ( Suppl 2 ) S103-S107 2021年12月 [国際誌]

Recently, radical vaginal hysterectomy (RVH) has developed into laparoscopically assisted radical vaginal hysterectomy (LARVH), which is associated with the laparoscopical procedure, and it is applied as radical vaginal trachelectomy and semi-radical vaginal hysterectomy. LARVH is indicated for patients with stage IB1 and IIA1 cervical carcinoma, especially those with a tumor size of less than 2 cm, because the cardinal ligaments cannot be resected widely. Although RVH that is associated with laparoscopic pelvic lymphadenectomy is the most used surgical procedure, radical trachelectomy may be performed either abdominally or vaginally (laparoscopic or robotic). One report found that the pregnancy rate was higher in patients who underwent minimally invasive or radical vaginal trachelectomy than in those who underwent radical abdominal trachelectomy.

DOI PubMed

マイクロバイオーム最前線-ヒト常在菌叢と各種疾患との関連- 腟内および子宮内細菌叢Dysbiosisへの真なるアプローチとは?

真里谷奨, 島野敏司, 遠藤俊明, 和田真一郎, 山田秀人, 藤井美穂, 水無瀬昂, 桜庭喜行, 鳥越俊彦, 齋藤豪

日本臨床微生物学会雑誌 ( (一社)日本臨床微生物学会 ) 32 ( Suppl.1 ) 164 - 164 2021年12月

低侵襲手術時代における子宮体癌に対するリンパ節摘出の意義と方法早期子宮体癌に対するリンパ節郭清の意義と術式の選択

松浦基樹, 玉手雅人, 長尾沙智子, 西村庸子, 秋元太志, 幅田周太朗, 寺田倫子, 齋藤豪

日本内視鏡外科学会雑誌 ( (一社)日本内視鏡外科学会 ) 26 ( 7 ) WS10 - 2 2021年12月

妊娠女性の頸部細胞診妊娠中施行radical trachelectomy症例における妊娠中細胞診の変化

石岡伸一, 新開翔太, 真里谷奨, 玉手雅人, 幅田周太朗, 松浦基樹, 岩崎雅宏, 齋藤豪

日本臨床細胞学会雑誌 ( (公社)日本臨床細胞学会 ) 60 ( Suppl.2 ) 458 - 458 2021年10月

骨髄間葉系間質細胞は子宮内膜症の病態に関与しているか?

幅田周太朗, 玉手雅人, 松浦基樹, 岩崎雅宏, 齋藤豪

日本臨床細胞学会雑誌 ( (公社)日本臨床細胞学会 ) 60 ( Suppl.2 ) 563 - 563 2021年10月

腹腔鏡下子宮全摘術中に腟パイプの直腸誤挿入により直腸損傷を生じた1例

伊野善彦, 玉手雅人, 篠原由梨, 西尾空, 阿部秀悦, 齋藤豪

日本産科婦人科内視鏡学会雑誌 ( (一社)日本産科婦人科内視鏡学会 ) 37 ( Suppl.I ) 274 - 274 2021年09月

後腟円蓋より突出する腟腫瘍に対し腹腔鏡下手術を行い、表在性筋線維芽細胞腫と判明した1例

鹿内智史, 松浦基樹, 梅本美菜, 杉田奈穂子, 鈴木将裕, 齋藤豪

日本産科婦人科内視鏡学会雑誌 ( (一社)日本産科婦人科内視鏡学会 ) 37 ( Suppl.I ) 376 - 376 2021年09月

Prediction of treatment response from the microenvironment of tumor immunity in cervical cancer patients treated with chemoradiotherapy.

Masanori Someya, Takaaki Tsuchiya, Yuki Fukushima, Tomokazu Hasegawa, Masakazu Hori, Mio Kitagawa, Toshio Gocho, Shoh Mafune, Yutaro Ikeuchi, Yoshihiko Hirohashi, Toshihiko Torigoe, Masahiro Iwasaki, Motoki Matsuura, Tsuyoshi Saito, Yoshihisa Matsumoto, Koh-Ichi Sakata

Medical molecular morphology 54 ( 3 ) 245 - 252 2021年09月 [国内誌]

To supplement clinical decision-making in the management of cervical cancer, various prognostic factors, including tumor immune microenvironments, were examined in patients with cervical cancer treated with definitive chemoradiotherapy. We retrospectively analyzed the expression of CD8, FoxP3, HLA-1, PD-L1, and XRCC4 in 100 cases of cervical cancer. The observed tumor immune microenvironments were also classified into three types: inflamed, excluded, and cold type. Less FoxP3+ T cells and cold-type tumor were found to be poor prognostic factors in addition to non-SCC, large pre-treatment tumor volume, and three or less cycles of concurrent chemotherapy based on multivariate analysis. Cold-type tumors had significantly worse prognoses than the other two types, whereas inflamed- and excluded-type tumors showed similar 5-year disease-specific survival (P < 0.001; 0% vs. 60.3% vs. 72.3%). Radiotherapy could overcome the inhibitory immune microenvironment that occurs in excluded type. Individualized combination therapy adapted to pre-treatment tumor immunity may be necessary to improve radiotherapy outcomes in cervical cancer.

DOI PubMed

腹腔鏡下に治療し得た非交通性副角妊娠の一例

和田渚, 春日芙貴子, 磯山響子, 常松梨紗, 玉手雅人, 幅田周太朗, 寺田倫子, 松浦基樹, 岩崎雅宏, 齋藤豪

日本産科婦人科内視鏡学会雑誌 ( (一社)日本産科婦人科内視鏡学会 ) 37 ( Suppl.I ) 225 - 225 2021年09月

異所性妊娠破裂による出血性ショックに対して、REBOAを施行し、安全に腹腔鏡下手術をなしえた1例

岩崎雅宏, 古来愛香, 幅田周太朗, 池田桂子, 野藤五沙, 野呂薫, 玉手雅人, 松浦基樹, 秋元太志, 磯山響子, 西村庸子, 和田渚, 齋藤豪

日本産科婦人科内視鏡学会雑誌 ( (一社)日本産科婦人科内視鏡学会 ) 37 ( Suppl.I ) 309 - 309 2021年09月

PGT-SRの結果とStengel-R法による主たる不均衡型転座の予測との一致率

遠藤俊明, 馬場剛, 久野芳佳, 池田詩子, 本間寛之, 逸見博文, 稲木誠, 原鐵晃, 尾崎守, 倉橋浩樹, 斎藤豪

日本IVF学会誌 ( (一社)日本IVF学会 ) 24 ( 2 ) 60 - 60 2021年09月

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